AlfalfaGEDB | Msa0827820

M. sativa cultivar zhongmu-4 / Msa0827820

KEGG: K00924|K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009909 GO:0009910 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048519 GO:0048580 GO:0048581 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051716 GO:0060089 GO:0060860 GO:0060862 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:2000026 GO:2000241 GO:2000242
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827820	RHN54439.1	96.010	401	13	2	1	398	56	456	0.0	787

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827820	sp\|P27450\|CST_ARATH	60.563	355	133	3	26	378	22	371	3.03e-159	457

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827820	A0A396HMA1	96.010	401	13	2	1	398	56	456	0.0	787

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000820	Msa0827820	0.807366	5.234839e-50	-8.615850e-47
Msa0101240	Msa0827820	0.801800	7.590166e-49	-8.615850e-47
Msa0151220	Msa0827820	0.800689	1.281157e-48	-8.615850e-47
Msa0184410	Msa0827820	0.927526	1.039957e-91	-8.615850e-47
Msa0244890	Msa0827820	0.812231	4.703685e-51	-8.615850e-47
Msa0259440	Msa0827820	0.818533	1.865049e-52	-8.615850e-47
Msa0259580	Msa0827820	0.818090	2.349748e-52	-8.615850e-47
Msa0305620	Msa0827820	0.804287	2.322727e-49	-8.615850e-47
Msa0463490	Msa0827820	0.800598	1.336759e-48	-8.615850e-47
Msa0543130	Msa0827820	0.802302	5.983972e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827820	MtrunA17_Chr5g0406841	96.010	401	13	2	1	398	56	456	0.0	787
Msa0827820	MtrunA17_Chr8g0370461	66.416	399	128	3	1	395	1	397	0.0	526
Msa0827820	MtrunA17_Chr8g0370571	68.103	348	106	4	49	395	18	361	5.76e-169	477
Msa0827820	MtrunA17_Chr3g0143951	60.833	360	132	5	28	387	26	376	6.74e-147	422
Msa0827820	MtrunA17_Chr2g0328171	52.369	401	167	7	1	394	1	384	4.24e-145	418
Msa0827820	MtrunA17_Chr5g0448661	62.069	319	114	2	63	381	80	391	2.17e-143	413
Msa0827820	MtrunA17_Chr5g0435211	50.000	378	172	4	1	377	1	362	7.49e-141	407
Msa0827820	MtrunA17_Chr5g0416311	57.975	326	131	3	65	388	53	374	4.99e-136	395
Msa0827820	MtrunA17_Chr3g0111631	49.869	381	175	4	1	381	3	367	3.45e-134	390
Msa0827820	MtrunA17_Chr7g0238671	54.381	331	149	1	65	395	50	378	1.87e-133	388

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827820	AT4G35600.1	60.563	355	133	3	26	378	22	371	3.08e-160	457
Msa0827820	AT4G35600.2	60.563	355	133	3	26	378	23	372	3.71e-160	457
Msa0827820	AT2G17220.1	65.723	318	105	2	64	381	63	376	4.24e-157	449
Msa0827820	AT2G17220.2	65.723	318	105	2	64	381	62	375	5.04e-157	449
Msa0827820	AT1G76360.1	54.167	384	163	5	6	381	74	452	2.91e-145	421
Msa0827820	AT2G28930.2	59.236	314	126	1	65	378	46	357	3.02e-139	404
Msa0827820	AT2G28930.3	59.236	314	126	1	65	378	49	360	5.05e-139	403
Msa0827820	AT2G28930.1	59.236	314	126	1	65	378	57	368	8.95e-139	403
Msa0827820	AT1G07570.1	55.143	350	151	2	33	378	9	356	6.50e-138	400
Msa0827820	AT1G07570.5	55.143	350	151	2	33	378	9	356	6.50e-138	400

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Find 89 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTGGTTCTGGAATGA+TGG	0.549508	5_3:+69338081	Msa0827820:CDS
AACCCCATCAAGAAGTATAT+TGG	0.344165	5_3:-69338989	None:intergenic
AAGAGTAAAGTTTGGGAGAT+TGG	0.283361	5_3:+69337863	Msa0827820:CDS
AAGAGTGATGTTTATGGATT+TGG	0.319515	5_3:+69339681	Msa0827820:CDS
AAGGAAGCATAGAGATAAAT+GGG	0.382120	5_3:+69337909	Msa0827820:CDS
AAGGATTTCTCACCCCAACT+TGG	0.530218	5_3:+69338655	Msa0827820:CDS
AATGCAAAGATTTCAGATTT+TGG	0.128514	5_3:+69339419	Msa0827820:CDS
AATTTGAAGGTGTTTAGTTA+TGG	0.389583	5_3:+69337950	Msa0827820:CDS
ACACTGGCACCTGCAAAAGC+TGG	0.579978	5_3:+69338067	Msa0827820:CDS
ACCTAGTAGCTTCACCAAGT+TGG	0.468552	5_3:-69338669	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTATGAATATTGAGGGAAGA+GGG	-	chr5_3:69337543-69337562	None:intergenic	30.0%
CTCAATATTCATAACACTTC+AGG	+	chr5_3:69337549-69337568	Msa0827820:CDS	30.0%
AAAATTGTTACTGGGCAAAT+TGG	+	chr5_3:69337705-69337724	Msa0827820:intron	30.0%
AAATTGTTACTGGGCAAATT+GGG	+	chr5_3:69337706-69337725	Msa0827820:intron	30.0%
TCTAGAAATGCCAAATTTGA+AGG	+	chr5_3:69337937-69337956	Msa0827820:CDS	30.0%
GTTTAGTTATGGAGATTTGA+AGG	+	chr5_3:69337961-69337980	Msa0827820:CDS	30.0%
TTGGTAAAGTGTATAAAGGT+TGG	+	chr5_3:69338032-69338051	Msa0827820:CDS	30.0%
TGTGAACCTCAATATAATAG+AGG	+	chr5_3:69338475-69338494	Msa0827820:intron	30.0%
TAGAGGATTACAATCAAATG+CGG	+	chr5_3:69338492-69338511	Msa0827820:intron	30.0%
CTTCCAATATACTTCTTGAT+GGG	+	chr5_3:69338986-69339005	Msa0827820:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	69337489	69340260	69337489	ID=Msa0827820;Name=Msa0827820
chr5_3	mRNA	69337489	69340260	69337489	ID=Msa0827820-mRNA-1;Parent=Msa0827820;Name=Msa0827820-mRNA-1;_AED=0.08;_eAED=0.08;_QI=12\|1\|1\|1\|1\|1\|6\|220\|398
chr5_3	exon	69337489	69337570	69337489	ID=Msa0827820-mRNA-1:exon:21811;Parent=Msa0827820-mRNA-1
chr5_3	exon	69337807	69338156	69337807	ID=Msa0827820-mRNA-1:exon:21812;Parent=Msa0827820-mRNA-1
chr5_3	exon	69338635	69338770	69338635	ID=Msa0827820-mRNA-1:exon:21813;Parent=Msa0827820-mRNA-1
chr5_3	exon	69338866	69339008	69338866	ID=Msa0827820-mRNA-1:exon:21814;Parent=Msa0827820-mRNA-1
chr5_3	exon	69339413	69339536	69339413	ID=Msa0827820-mRNA-1:exon:21815;Parent=Msa0827820-mRNA-1
chr5_3	exon	69339667	69340260	69339667	ID=Msa0827820-mRNA-1:exon:21816;Parent=Msa0827820-mRNA-1
chr5_3	five_prime_UTR	69337489	69337500	69337489	ID=Msa0827820-mRNA-1:five_prime_utr;Parent=Msa0827820-mRNA-1
chr5_3	CDS	69337501	69337570	69337501	ID=Msa0827820-mRNA-1:cds;Parent=Msa0827820-mRNA-1
chr5_3	CDS	69337807	69338156	69337807	ID=Msa0827820-mRNA-1:cds;Parent=Msa0827820-mRNA-1
chr5_3	CDS	69338635	69338770	69338635	ID=Msa0827820-mRNA-1:cds;Parent=Msa0827820-mRNA-1
chr5_3	CDS	69338866	69339008	69338866	ID=Msa0827820-mRNA-1:cds;Parent=Msa0827820-mRNA-1
chr5_3	CDS	69339413	69339536	69339413	ID=Msa0827820-mRNA-1:cds;Parent=Msa0827820-mRNA-1
chr5_3	CDS	69339667	69340040	69339667	ID=Msa0827820-mRNA-1:cds;Parent=Msa0827820-mRNA-1
chr5_3	three_prime_UTR	69340041	69340260	69340041	ID=Msa0827820-mRNA-1:three_prime_utr;Parent=Msa0827820-mRNA-1

Gene Sequence

>Msa0827820

ATGGGTCTTTGCTTCACCTCACTCTCTCACCAATCACCACCCTCTTCCCTCAATATTCATAACACTTCAGGGTTTGGGAGTAATAATGATGATAGTACAAAAGAAGAGTCATCATCATCATGTAATAAGAGTAAAGTTTGGGAGATTGGAAATAGTGAAAGTGATAGTAGAGAAGGAAGCATAGAGATAAATGGGAAGTTTCTAGAAATGCCAAATTTGAAGGTGTTTAGTTATGGAGATTTGAAGGCTGCAACAAAGAGTTTCAAGTCAGATGCATTACTTGGTGAAGGAGGTTTTGGTAAAGTGTATAAAGGTTGGTTAAATGCTGTGACACTGGCACCTGCAAAAGCTGGTTCTGGAATGATGGTTGCTATCAAGAAATTGAAACGTGATAGTGTTCAAGGTCTTCAAGAATGGCAGTCAGAAATAAACTTTTTAGGAAGGATTTCTCACCCCAACTTGGTGAAGCTACTAGGTTACTGTAGGGACAACGATGAATTTCTCCTTGTGTACGAGTTCATGCCAAGGGGAAGCTTGGAGAATCATCTCTTTAGAAGAAATACTAACATAGAGCCACTTTCTTGGAACACTCGACTCAAAATAGCTACCGATGCAGCTCGAGGCTTAGCTTTTTTGCACTCCTCAGATAAGCAAGTCATATATAGAGATTTCAAGGCTTCCAATATACTTCTTGATGGGAATTACAATGCAAAGATTTCAGATTTTGGGTTGGCTAAATTTGGGCCGTCTGGGGGAGATTCGCACGTGACTACTAGGATCATGGGCACATATGGTTATGCTGCCCCTGAATACATGGCAACAGGTCATTTGTATGTGAAGAGTGATGTTTATGGATTTGGTGTAGTGCTGCTTGAAATGTTGACAGGATTGCAAGCATTTGATTCAAAGAGGCCTGAAGGGCAGCAAAACCTAATTGAATGGATTAAGCCATCTCTCTCTGATAAAAGAAAGTTGAAAAGTAACAACATTGTGGATTACAGGTTAGAGGGTCAATATACATCAAAGGCAGCATTTGAAACAGCACACCTTATTCTTCAATGCCTTCAACCTGACCCTAAAAAACGTCCTTCAATGAAAGATGTTTTAGGGATATTGGAAGGCATAAAAGCTATCAAGAATAAAAGGAAGATATCCAAGAATCATTGTACTAAGTCTGCAACTATGGATAATTTATAA

Protein sequence

>Msa0827820

MGLCFTSLSHQSPPSSLNIHNTSGFGSNNDDSTKEESSSSCNKSKVWEIGNSESDSREGSIEINGKFLEMPNLKVFSYGDLKAATKSFKSDALLGEGGFGKVYKGWLNAVTLAPAKAGSGMMVAIKKLKRDSVQGLQEWQSEINFLGRISHPNLVKLLGYCRDNDEFLLVYEFMPRGSLENHLFRRNTNIEPLSWNTRLKIATDAARGLAFLHSSDKQVIYRDFKASNILLDGNYNAKISDFGLAKFGPSGGDSHVTTRIMGTYGYAAPEYMATGHLYVKSDVYGFGVVLLEMLTGLQAFDSKRPEGQQNLIEWIKPSLSDKRKLKSNNIVDYRLEGQYTSKAAFETAHLILQCLQPDPKKRPSMKDVLGILEGIKAIKNKRKISKNHCTKSATMDNL*