AlfalfaGEDB | Msa0000820

M. sativa cultivar zhongmu-4 / Msa0000820

KEGG: -
GO: GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010033 GO:0010035 GO:0010200 GO:0010243 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051865 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901698 GO:1901700
PFAM: PF04564.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0000820	sp\|Q9SVC6\|PUB22_ARATH	59.132	438	154	7	1	417	1	434	4.45e-179	509

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000820	Msa0004480	0.802496	5.457103e-49	-8.615850e-47
Msa0000820	Msa0011050	0.813179	2.918453e-51	-8.615850e-47
Msa0000820	Msa0037760	0.816207	6.227825e-52	-8.615850e-47
Msa0000820	Msa0090380	0.801896	7.253810e-49	-8.615850e-47
Msa0000820	Msa0104760	0.816095	6.599266e-52	-8.615850e-47
Msa0000820	Msa0104810	0.829535	4.874428e-55	-8.615850e-47
Msa0000820	Msa0119430	0.815376	9.543113e-52	-8.615850e-47
Msa0000820	Msa0137860	0.802991	4.313858e-49	-8.615850e-47
Msa0000820	Msa0151220	0.831600	1.522367e-55	-8.615850e-47
Msa0000820	Msa0223140	0.809364	1.962724e-50	-8.615850e-47
Msa0000820	Msa0245400	0.812994	3.203485e-51	-8.615850e-47
Msa0000820	Msa0259440	0.830040	3.673524e-55	-8.615850e-47
Msa0000820	Msa0259580	0.826241	3.023023e-54	-8.615850e-47
Msa0000820	Msa0260530	0.808096	3.662619e-50	-8.615850e-47
Msa0000820	Msa0305620	0.819639	1.044748e-52	-8.615850e-47
Msa0000820	Msa0339720	0.812250	4.659907e-51	-8.615850e-47
Msa0000820	Msa0345980	0.819155	1.347445e-52	-8.615850e-47
Msa0000820	Msa0393100	0.836938	6.976935e-57	-8.615850e-47
Msa0000820	Msa0437070	0.831651	1.479035e-55	-8.615850e-47
Msa0000820	Msa0443450	0.821506	3.893246e-53	-8.615850e-47
Msa0000820	Msa0463490	0.830998	2.141189e-55	-8.615850e-47
Msa0000820	Msa0484800	0.814371	1.593743e-51	-8.615850e-47
Msa0000820	Msa0488580	0.829076	6.301772e-55	-8.615850e-47
Msa0000820	Msa0497860	0.802651	5.071564e-49	-8.615850e-47
Msa0000820	Msa0521380	0.800326	1.518619e-48	-8.615850e-47
Msa0000820	Msa0590390	0.806600	7.604396e-50	-8.615850e-47
Msa0000820	Msa0604570	0.801049	1.081419e-48	-8.615850e-47
Msa0000820	Msa0615200	0.815909	7.258930e-52	-8.615850e-47
Msa0000820	Msa0615210	0.837132	6.223638e-57	-8.615850e-47
Msa0000820	Msa0652600	0.800709	1.268896e-48	-8.615850e-47
Msa0000820	Msa0698280	0.823377	1.431584e-53	-8.615850e-47
Msa0000820	Msa0710810	0.805511	1.288783e-49	-8.615850e-47
Msa0000820	Msa0718800	0.802180	6.341435e-49	-8.615850e-47
Msa0000820	Msa0725580	0.805332	1.404977e-49	-8.615850e-47
Msa0000820	Msa0827820	0.807366	5.234839e-50	-8.615850e-47
Msa0000820	Msa0860250	0.837680	4.505823e-57	-8.615850e-47
Msa0000820	Msa0881750	0.803512	3.365442e-49	-8.615850e-47
Msa0000820	Msa0968050	0.822018	2.964318e-53	-8.615850e-47
Msa0000820	Msa1025750	0.822962	1.788487e-53	-8.615850e-47
Msa0000820	Msa1041250	0.800374	1.485047e-48	-8.615850e-47
Msa0000820	Msa1061970	0.815938	7.153586e-52	-8.615850e-47
Msa0000820	Msa1064510	0.835191	1.936805e-56	-8.615850e-47
Msa0000820	Msa1078330	0.833821	4.277849e-56	-8.615850e-47
Msa0000820	Msa1109670	0.824753	6.804189e-54	-8.615850e-47
Msa0000820	Msa1114670	0.803666	3.127058e-49	-8.615850e-47
Msa0000820	Msa1123720	0.830604	2.675045e-55	-8.615850e-47
Msa0000820	Msa1153230	0.813252	2.812088e-51	-8.615850e-47
Msa0000820	Msa1169860	0.835789	1.367435e-56	-8.615850e-47
Msa0000820	Msa1221220	0.801686	8.009973e-49	-8.615850e-47
Msa0000820	Msa1369320	0.819286	1.257877e-52	-8.615850e-47
Msa0000820	Msa1413800	0.845456	4.016205e-59	-8.615850e-47
Msa0000820	Msa1436090	0.846999	1.525680e-59	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0000820	MtrunA17_Chr7g0267501	98.337	421	6	1	1	420	1	421	0.0	850
Msa0000820	MtrunA17_Chr1g0177281	64.760	437	131	5	1	417	1	434	0.0	549
Msa0000820	MtrunA17_Chr1g0177261	64.989	437	130	5	1	417	1	434	0.0	549
Msa0000820	MtrunA17_Chr1g0177271	64.531	437	132	5	1	417	1	434	0.0	521
Msa0000820	MtrunA17_Chr2g0322021	58.413	416	157	5	4	417	2	403	5.23e-159	454
Msa0000820	MtrunA17_Chr1g0177301	43.510	416	219	6	4	415	2	405	1.65e-111	333
Msa0000820	MtrunA17_Chr4g0025501	40.000	425	226	10	5	416	3	411	1.75e-94	290
Msa0000820	MtrunA17_Chr3g0110121	34.123	422	252	11	4	416	32	436	8.35e-60	201
Msa0000820	MtrunA17_Chr5g0434171	32.706	425	247	13	5	413	32	433	4.43e-56	191
Msa0000820	MtrunA17_Chr4g0044631	33.086	405	240	14	6	402	12	393	1.13e-49	173
Msa0000820	MtrunA17_Chr6g0479261	29.157	415	251	12	5	397	22	415	2.49e-49	173
Msa0000820	MtrunA17_Chr5g0420471	30.275	436	261	14	5	416	22	438	5.15e-49	172
Msa0000820	MtrunA17_Chr8g0384041	31.343	402	253	11	6	397	8	396	1.50e-40	149
Msa0000820	MtrunA17_Chr3g0129811	31.738	397	256	11	6	397	81	467	7.40e-39	145
Msa0000820	MtrunA17_Chr4g0031431	29.440	411	247	12	2	400	249	628	1.82e-35	138
Msa0000820	MtrunA17_Chr5g0400971	27.068	399	265	8	7	405	66	438	4.16e-32	126
Msa0000820	MtrunA17_Chr1g0199321	30.479	292	174	8	8	291	247	517	1.36e-28	118
Msa0000820	MtrunA17_Chr7g0237931	26.260	377	238	9	8	370	237	587	8.69e-27	112
Msa0000820	MtrunA17_Chr4g0044411	29.642	307	177	11	4	296	269	550	3.82e-26	110
Msa0000820	MtrunA17_Chr1g0152291	27.962	422	257	12	7	415	44	431	4.76e-26	109
Msa0000820	MtrunA17_Chr7g0248251	30.612	294	166	10	8	291	274	539	1.23e-25	109
Msa0000820	MtrunA17_Chr2g0284461	27.036	307	178	9	6	291	290	571	2.08e-24	105
Msa0000820	MtrunA17_Chr5g0437411	25.266	376	230	13	6	355	291	641	7.57e-24	104
Msa0000820	MtrunA17_Chr4g0059201	28.750	400	256	15	9	397	320	701	9.64e-24	103
Msa0000820	MtrunA17_Chr3g0117851	29.363	361	223	14	9	353	276	620	6.29e-23	101
Msa0000820	MtrunA17_Chr5g0413681	28.226	372	231	15	12	368	284	634	2.82e-22	99.4
Msa0000820	MtrunA17_Chr4g0013091	28.358	268	152	10	5	251	310	558	2.11e-20	94.0
Msa0000820	MtrunA17_Chr4g0048811	27.879	330	164	11	8	337	5	260	2.58e-19	88.2
Msa0000820	MtrunA17_Chr2g0280671	27.526	287	181	8	14	295	21	285	1.52e-18	86.7
Msa0000820	MtrunA17_Chr7g0275081	26.821	302	183	12	4	292	74	350	7.64e-17	82.4
Msa0000820	MtrunA17_Chr3g0122721	44.186	86	40	3	9	94	281	358	1.53e-16	82.0
Msa0000820	MtrunA17_Chr7g0234671	48.148	81	37	3	6	86	76	151	1.66e-16	81.3
Msa0000820	MtrunA17_Chr1g0183781	47.222	72	36	2	6	77	239	308	4.96e-16	80.5
Msa0000820	MtrunA17_Chr7g0261371	46.667	75	38	2	6	80	239	311	1.05e-15	79.3
Msa0000820	MtrunA17_Chr8g0390311	36.441	118	66	2	9	118	396	512	1.18e-15	79.7
Msa0000820	MtrunA17_Chr8g0377151	23.831	449	272	15	9	404	283	714	1.69e-14	75.5
Msa0000820	MtrunA17_Chr3g0144071	25.168	298	177	9	12	299	34	295	5.58e-14	73.9
Msa0000820	MtrunA17_Chr8g0373031	26.720	378	216	19	9	375	276	603	1.08e-13	73.2
Msa0000820	MtrunA17_Chr1g0196911	50.000	68	33	1	9	76	507	573	1.82e-13	72.8
Msa0000820	MtrunA17_Chr3g0106931	46.053	76	37	3	6	80	231	303	2.27e-13	72.0
Msa0000820	MtrunA17_Chr1g0189811	38.235	102	57	4	1	102	56	151	3.37e-13	70.9
Msa0000820	MtrunA17_Chr5g0412171	45.714	70	36	2	7	76	731	798	5.05e-13	71.2
Msa0000820	MtrunA17_Chr2g0328101	46.575	73	37	2	4	76	736	806	2.48e-12	68.9
Msa0000820	MtrunA17_Chr5g0404311	40.909	88	46	4	4	91	269	350	3.05e-12	68.6
Msa0000820	MtrunA17_Chr1g0202971	46.479	71	36	2	9	79	649	717	6.08e-12	67.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0000820	AT3G52450.1	59.132	438	154	7	1	417	1	434	4.52e-180	509
Msa0000820	AT2G35930.1	60.000	420	150	7	1	417	6	410	8.56e-177	499
Msa0000820	AT3G11840.1	37.472	451	234	7	2	414	4	444	6.90e-89	277
Msa0000820	AT3G19380.1	35.385	390	226	8	6	386	12	384	1.02e-61	206
Msa0000820	AT1G49780.1	33.735	415	251	10	6	411	12	411	2.80e-56	191
Msa0000820	AT1G66160.1	28.537	417	231	10	5	397	30	403	1.80e-46	166
Msa0000820	AT5G37490.1	30.424	401	257	12	3	397	26	410	3.78e-46	165
Msa0000820	AT3G18710.1	30.392	408	259	12	1	397	1	394	6.30e-34	131
Msa0000820	AT1G66160.2	25.899	417	226	11	5	397	30	387	1.01e-33	130
Msa0000820	AT5G09800.1	27.848	395	262	9	8	397	11	387	4.06e-33	129
Msa0000820	AT1G23030.1	29.808	312	184	10	4	307	237	521	1.33e-31	127
Msa0000820	AT1G71020.2	28.750	320	186	9	4	307	91	384	2.48e-30	122
Msa0000820	AT1G71020.1	28.750	320	186	9	4	307	239	532	6.20e-30	122
Msa0000820	AT3G46510.1	28.467	411	239	13	8	400	256	629	2.03e-29	121
Msa0000820	AT5G65920.1	27.543	403	258	10	7	405	59	431	3.23e-29	119
Msa0000820	AT5G65920.2	27.543	403	258	10	7	405	59	431	3.23e-29	119
Msa0000820	AT5G64660.1	27.494	411	261	13	6	398	8	399	8.07e-29	117
Msa0000820	AT3G49810.1	26.650	394	257	9	7	397	63	427	1.57e-28	117
Msa0000820	AT5G42340.1	30.605	281	167	9	8	284	290	546	9.13e-27	113
Msa0000820	AT2G28830.1	30.744	309	172	11	2	293	178	461	3.41e-26	111
Msa0000820	AT3G54850.1	28.328	293	180	8	8	291	248	519	5.39e-26	110
Msa0000820	AT1G29340.1	26.570	414	256	14	6	400	303	687	1.59e-24	106
Msa0000820	AT4G21350.1	28.739	341	207	10	6	341	3	312	8.47e-23	99.8
Msa0000820	AT1G10560.1	26.979	341	213	11	2	326	280	600	4.89e-22	99.4
Msa0000820	AT5G01830.1	28.276	290	175	9	5	284	271	537	1.14e-19	92.0
Msa0000820	AT1G60190.1	26.524	328	205	12	14	326	284	590	1.62e-18	88.6
Msa0000820	AT3G02840.1	26.377	345	223	13	85	412	40	370	2.85e-17	83.6
Msa0000820	AT3G07360.1	27.027	296	180	10	6	287	72	345	7.67e-17	82.8
Msa0000820	AT1G67530.3	47.059	85	39	3	9	93	273	351	5.36e-16	80.5
Msa0000820	AT5G18320.1	29.617	287	182	8	2	284	66	336	6.26e-16	79.7
Msa0000820	AT1G67530.2	47.059	85	39	3	9	93	273	351	6.78e-16	80.5
Msa0000820	AT1G67530.1	47.059	85	39	3	9	93	273	351	6.78e-16	80.5
Msa0000820	AT1G67530.4	47.059	85	39	3	9	93	273	351	6.78e-16	80.5
Msa0000820	AT1G27910.1	47.059	85	39	3	9	93	280	358	3.23e-15	78.2
Msa0000820	AT5G65500.2	46.914	81	41	2	4	84	719	797	4.16e-15	78.2
Msa0000820	AT5G65500.1	46.914	81	41	2	4	84	711	789	4.18e-15	78.2
Msa0000820	AT1G24330.1	47.059	85	39	4	9	93	276	354	8.79e-15	77.0
Msa0000820	AT1G24330.2	47.059	85	39	4	9	93	276	354	8.79e-15	77.0
Msa0000820	AT5G18340.1	28.296	311	187	13	2	299	68	355	1.23e-14	75.9
Msa0000820	AT3G54790.2	44.000	75	40	2	6	80	200	272	9.81e-14	73.6
Msa0000820	AT1G01660.2	42.466	73	40	2	9	81	499	569	1.06e-13	73.2
Msa0000820	AT1G01660.1	42.466	73	40	2	9	81	498	568	1.08e-13	73.2
Msa0000820	AT5G67340.1	46.667	75	38	2	6	80	238	310	1.11e-13	73.6
Msa0000820	AT5G67340.2	46.667	75	38	2	6	80	238	310	1.11e-13	73.6
Msa0000820	AT3G54790.3	44.000	75	40	2	6	80	236	308	1.26e-13	73.6
Msa0000820	AT3G54790.1	44.000	75	40	2	6	80	236	308	1.26e-13	73.6
Msa0000820	AT2G45920.1	44.737	76	40	2	7	82	324	397	2.13e-13	72.0
Msa0000820	AT5G57035.1	43.478	69	37	2	8	76	708	774	4.39e-13	71.6
Msa0000820	AT2G23140.1	37.634	93	52	3	12	104	237	323	8.34e-13	70.9
Msa0000820	AT2G23140.2	37.634	93	52	3	12	104	234	320	8.55e-13	70.9
Msa0000820	AT2G23140.4	37.634	93	52	3	12	104	234	320	8.55e-13	70.9
Msa0000820	AT1G68940.2	27.564	156	82	4	12	158	303	436	9.20e-13	70.9
Msa0000820	AT2G23140.3	37.634	93	52	3	12	104	45	131	9.46e-13	70.5
Msa0000820	AT2G23140.5	37.634	93	52	3	12	104	45	131	9.46e-13	70.5
Msa0000820	AT1G68940.3	27.564	156	82	4	12	158	250	383	9.54e-13	70.9
Msa0000820	AT1G68940.1	27.564	156	82	4	12	158	303	436	1.04e-12	70.9
Msa0000820	AT4G36550.1	43.243	74	40	2	8	81	219	290	1.37e-12	70.1
Msa0000820	AT2G19410.3	45.070	71	36	2	6	76	709	776	3.12e-12	68.9
Msa0000820	AT2G19410.2	45.070	71	36	2	6	76	744	811	3.48e-12	68.9
Msa0000820	AT2G19410.1	45.070	71	36	2	6	76	723	790	3.53e-12	68.9
Msa0000820	AT3G61390.4	41.096	73	41	2	7	79	277	347	6.55e-12	67.0
Msa0000820	AT2G45910.2	44.928	69	36	2	8	76	763	829	6.96e-12	68.2
Msa0000820	AT2G45910.1	44.928	69	36	2	8	76	763	829	6.96e-12	68.2
Msa0000820	AT3G61390.2	41.096	73	41	2	7	79	352	422	7.36e-12	67.4
Msa0000820	AT5G18330.1	27.513	378	242	16	2	372	59	411	7.36e-12	67.4
Msa0000820	AT1G76390.1	42.029	69	35	2	12	76	29	96	1.16e-11	67.4
Msa0000820	AT1G76390.2	42.029	69	35	2	12	76	29	96	1.16e-11	67.4
Msa0000820	AT1G01680.1	46.377	69	35	2	12	80	237	303	1.41e-11	65.5
Msa0000820	AT1G20780.1	41.096	73	38	2	12	80	27	98	2.29e-11	66.2
Msa0000820	AT1G20780.2	41.096	73	38	2	12	80	27	98	2.29e-11	66.2
Msa0000820	AT1G20780.3	41.096	73	38	2	12	80	27	98	2.29e-11	66.2

Find 51 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAATTAATTTCACTGGTAAT+TGG	0.118200	1_1:+2130783	None:intergenic
CTTCATGATTTCTAATGATA+TGG	0.167930	1_1:+2131989	None:intergenic
TTGTTTCTAAGAAGATTCTA+AGG	0.280230	1_1:-2131044	Msa0000820:CDS
AATTAATTTCACTGGTAATT+GGG	0.298853	1_1:+2130784	None:intergenic
AGACTTAGATCTATGGCTTC+TGG	0.311800	1_1:-2131672	Msa0000820:CDS
ATACCTGTTGAGACTGTTAC+TGG	0.321164	1_1:+2131963	None:intergenic
CCCATGGACAGAGTGCAATT+AGG	0.328586	1_1:+2131241	None:intergenic
CTTGAAAAGACTTAGATCTA+TGG	0.330588	1_1:-2131679	Msa0000820:CDS
AGCATTTCTTGAACAACATT+AGG	0.349387	1_1:+2130955	None:intergenic
AAAACCAACATCATTTCATT+TGG	0.354293	1_1:+2131135	None:intergenic
TTCATGATTTCTAATGATAT+GGG	0.374449	1_1:+2131990	None:intergenic
CCATGGACAGAGTGCAATTA+GGG	0.383112	1_1:+2131242	None:intergenic
AACAAAAGTTCTATTAAAAC+AGG	0.395977	1_1:+2131180	None:intergenic
CCCTAATTGCACTCTGTCCA+TGG	0.442705	1_1:-2131242	Msa0000820:CDS
ATTGACTTTGTTAACAACTC+TGG	0.442706	1_1:-2131567	Msa0000820:CDS
TTGATTCTGTTTCTTCCCCA+TGG	0.450469	1_1:+2131225	None:intergenic
AAACTACTTGCTTTCAGAAA+CGG	0.455035	1_1:-2131465	Msa0000820:CDS
AATCTTCATGTGTCTGAAGC+TGG	0.462024	1_1:-2131495	Msa0000820:CDS
TGTTGTGGCTAAGCTTTGCT+TGG	0.464959	1_1:-2130923	Msa0000820:CDS
AGAATCAAAGGTGTTGAAGC+TGG	0.489133	1_1:-2131210	Msa0000820:CDS
TTAACAAAAGTTATGCAGAA+AGG	0.492717	1_1:-2131426	Msa0000820:CDS
GATCCAGTAACAGTCTCAAC+AGG	0.492806	1_1:-2131966	Msa0000820:CDS
AGATACTTAAGATGCATGCT+AGG	0.498217	1_1:-2130855	Msa0000820:CDS
ATTAATTTCACTGGTAATTG+GGG	0.513713	1_1:+2130785	None:intergenic
AATCTTTGTCAGTGTGCTGA+AGG	0.523947	1_1:-2131105	Msa0000820:CDS
ACGCTTCACAAGGCATTGAA+AGG	0.524077	1_1:-2131794	Msa0000820:CDS
CCTAATTGCACTCTGTCCAT+GGG	0.527316	1_1:-2131241	Msa0000820:CDS
TTAGAAGACTCATTCAAGCT+TGG	0.528792	1_1:-2131827	Msa0000820:CDS
AAAACCAAATGAAATGATGT+TGG	0.529378	1_1:-2131139	Msa0000820:CDS
TATGAAGAAAGCAAATTACT+AGG	0.534963	1_1:+2130808	None:intergenic
AGCAAATTACTAGGTATGCA+TGG	0.539218	1_1:+2130817	None:intergenic
GTTCAAGAAATGCTTAAACT+TGG	0.545979	1_1:-2130946	Msa0000820:CDS
GAATGAGTCTTCTAAGAGTG+TGG	0.559382	1_1:+2131835	None:intergenic
TGGGGAAGAAACAGAATCAA+AGG	0.566142	1_1:-2131222	Msa0000820:CDS
AGTCTTCTAAGAGTGTGGTT+TGG	0.569998	1_1:+2131840	None:intergenic
TGATTGTGGAAGTAAGACTA+AGG	0.572296	1_1:-2130890	Msa0000820:CDS
TTAAGATGCATGCTAGGGCA+TGG	0.573333	1_1:-2130849	Msa0000820:CDS
TGTTTCTAAGAAGATTCTAA+GGG	0.577022	1_1:-2131043	Msa0000820:CDS
TTCAATACTTTCTCTGTCGT+AGG	0.588046	1_1:+2131938	None:intergenic
AAACGTTGCATAGAAGATGC+AGG	0.588793	1_1:-2131636	Msa0000820:CDS
ATGGGGCAGACAAAGAATGA+AGG	0.611200	1_1:+2132008	None:intergenic
CATATCATTAGAAATCATGA+AGG	0.612177	1_1:-2131988	Msa0000820:CDS
TCATGATTTCTAATGATATG+GGG	0.613195	1_1:+2131991	None:intergenic
AATGCTTAAACTTGGTGTTG+TGG	0.614712	1_1:-2130938	Msa0000820:CDS
GATACTTAAGATGCATGCTA+GGG	0.623745	1_1:-2130854	Msa0000820:CDS
TGTACATTAAACGCTTCACA+AGG	0.640683	1_1:-2131804	Msa0000820:CDS
TTGGTTCTTCAAGTTGATTG+TGG	0.650079	1_1:-2130904	Msa0000820:CDS
TATGGATCATGATGAGATCG+AGG	0.658015	1_1:-2132033	Msa0000820:CDS
CTAATTGCACTCTGTCCATG+GGG	0.676561	1_1:-2131240	Msa0000820:CDS
AGATGCATGCTAGGGCATGG+AGG	0.701659	1_1:-2130846	Msa0000820:CDS
TTGCGAATGATAGAGCTGTG+AGG	0.775637	1_1:-2131011	Msa0000820:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTCATGATTTCTAATGATAT+GGG	+	chr1_1:2130837-2130856	None:intergenic	20.0%
!!	AACAAAAGTTCTATTAAAAC+AGG	+	chr1_1:2131647-2131666	None:intergenic	20.0%
!!	TTCATGATTTCTAATGATAT+GGG	+	chr1_1:2130837-2130856	None:intergenic	20.0%
!!	AACAAAAGTTCTATTAAAAC+AGG	+	chr1_1:2131647-2131666	None:intergenic	20.0%
!	TCATGATTTCTAATGATATG+GGG	+	chr1_1:2130836-2130855	None:intergenic	25.0%
!	CTTCATGATTTCTAATGATA+TGG	+	chr1_1:2130838-2130857	None:intergenic	25.0%
!	CATATCATTAGAAATCATGA+AGG	-	chr1_1:2130836-2130855	Msa0000820:CDS	25.0%
!!!	ACAGAGAAAGTATTGAAAAA+TGG	-	chr1_1:2130892-2130911	Msa0000820:CDS	25.0%
!!!	TTTGATGAGTTTTGTGATTT+GGG	+	chr1_1:2131087-2131106	None:intergenic	25.0%
!!	TTTCTGCATAACTTTTGTTA+AGG	+	chr1_1:2131399-2131418	None:intergenic	25.0%
!	TTAACAAAAGTTATGCAGAA+AGG	-	chr1_1:2131398-2131417	Msa0000820:CDS	25.0%
!!!	TGAATTAGTACAAGTTTTGA+AGG	-	chr1_1:2131520-2131539	Msa0000820:CDS	25.0%
!	AAAACCAAATGAAATGATGT+TGG	-	chr1_1:2131685-2131704	Msa0000820:CDS	25.0%
!	AAAACCAACATCATTTCATT+TGG	+	chr1_1:2131692-2131711	None:intergenic	25.0%
!!!	TTGTTTCTAAGAAGATTCTA+AGG	-	chr1_1:2131780-2131799	Msa0000820:CDS	25.0%
!!	TGTTTCTAAGAAGATTCTAA+GGG	-	chr1_1:2131781-2131800	Msa0000820:CDS	25.0%
!	TATGAAGAAAGCAAATTACT+AGG	+	chr1_1:2132019-2132038	None:intergenic	25.0%
!	TCATGATTTCTAATGATATG+GGG	+	chr1_1:2130836-2130855	None:intergenic	25.0%
!	CTTCATGATTTCTAATGATA+TGG	+	chr1_1:2130838-2130857	None:intergenic	25.0%
!	CATATCATTAGAAATCATGA+AGG	-	chr1_1:2130836-2130855	Msa0000820:CDS	25.0%
!!!	ACAGAGAAAGTATTGAAAAA+TGG	-	chr1_1:2130892-2130911	Msa0000820:CDS	25.0%
!!!	TTTGATGAGTTTTGTGATTT+GGG	+	chr1_1:2131087-2131106	None:intergenic	25.0%
!!	TTTCTGCATAACTTTTGTTA+AGG	+	chr1_1:2131399-2131418	None:intergenic	25.0%
!	TTAACAAAAGTTATGCAGAA+AGG	-	chr1_1:2131398-2131417	Msa0000820:CDS	25.0%
!!!	TGAATTAGTACAAGTTTTGA+AGG	-	chr1_1:2131520-2131539	Msa0000820:CDS	25.0%
!	AAAACCAAATGAAATGATGT+TGG	-	chr1_1:2131685-2131704	Msa0000820:CDS	25.0%
!	AAAACCAACATCATTTCATT+TGG	+	chr1_1:2131692-2131711	None:intergenic	25.0%
!!!	TTGTTTCTAAGAAGATTCTA+AGG	-	chr1_1:2131780-2131799	Msa0000820:CDS	25.0%
!!	TGTTTCTAAGAAGATTCTAA+GGG	-	chr1_1:2131781-2131800	Msa0000820:CDS	25.0%
!	TATGAAGAAAGCAAATTACT+AGG	+	chr1_1:2132019-2132038	None:intergenic	25.0%
!!!	GATAGCTGTTGTTTTGTAAT+AGG	+	chr1_1:2130945-2130964	None:intergenic	30.0%
!!	CTTTGATGAGTTTTGTGATT+TGG	+	chr1_1:2131088-2131107	None:intergenic	30.0%
	CTTGAAAAGACTTAGATCTA+TGG	-	chr1_1:2131145-2131164	Msa0000820:CDS	30.0%
!	ATTGACTTTGTTAACAACTC+TGG	-	chr1_1:2131257-2131276	Msa0000820:CDS	30.0%
	AAACTACTTGCTTTCAGAAA+CGG	-	chr1_1:2131359-2131378	Msa0000820:CDS	30.0%
!!!	TGAAATGATGTTGGTTTTGT+TGG	-	chr1_1:2131694-2131713	Msa0000820:CDS	30.0%
	AGCATTTCTTGAACAACATT+AGG	+	chr1_1:2131872-2131891	None:intergenic	30.0%
	GTTCAAGAAATGCTTAAACT+TGG	-	chr1_1:2131878-2131897	Msa0000820:CDS	30.0%
	TAAGACTAAGGAAAAAGCAA+GGG	-	chr1_1:2131946-2131965	Msa0000820:CDS	30.0%
!!!	GATAGCTGTTGTTTTGTAAT+AGG	+	chr1_1:2130945-2130964	None:intergenic	30.0%
!!	CTTTGATGAGTTTTGTGATT+TGG	+	chr1_1:2131088-2131107	None:intergenic	30.0%
	CTTGAAAAGACTTAGATCTA+TGG	-	chr1_1:2131145-2131164	Msa0000820:CDS	30.0%
!	ATTGACTTTGTTAACAACTC+TGG	-	chr1_1:2131257-2131276	Msa0000820:CDS	30.0%
	AAACTACTTGCTTTCAGAAA+CGG	-	chr1_1:2131359-2131378	Msa0000820:CDS	30.0%
!!!	TGAAATGATGTTGGTTTTGT+TGG	-	chr1_1:2131694-2131713	Msa0000820:CDS	30.0%
	AGCATTTCTTGAACAACATT+AGG	+	chr1_1:2131872-2131891	None:intergenic	30.0%
	GTTCAAGAAATGCTTAAACT+TGG	-	chr1_1:2131878-2131897	Msa0000820:CDS	30.0%
	TAAGACTAAGGAAAAAGCAA+GGG	-	chr1_1:2131946-2131965	Msa0000820:CDS	30.0%
	TTCAATACTTTCTCTGTCGT+AGG	+	chr1_1:2130889-2130908	None:intergenic	35.0%
	TTAGAAGACTCATTCAAGCT+TGG	-	chr1_1:2130997-2131016	Msa0000820:CDS	35.0%
	TGTACATTAAACGCTTCACA+AGG	-	chr1_1:2131020-2131039	Msa0000820:CDS	35.0%
!!	GTTTTGTTGATTGGTGGTTT+TGG	+	chr1_1:2131065-2131084	None:intergenic	35.0%
!!!	ATTTGGGTTTTGTTGATTGG+TGG	+	chr1_1:2131071-2131090	None:intergenic	35.0%
!!!	GTGATTTGGGTTTTGTTGAT+TGG	+	chr1_1:2131074-2131093	None:intergenic	35.0%
!	TTTTGATGTAGTAACTGCAC+TGG	+	chr1_1:2131485-2131504	None:intergenic	35.0%
!!	AATGCTTAAACTTGGTGTTG+TGG	-	chr1_1:2131886-2131905	Msa0000820:CDS	35.0%
!!	TTGGTTCTTCAAGTTGATTG+TGG	-	chr1_1:2131920-2131939	Msa0000820:CDS	35.0%
	TGATTGTGGAAGTAAGACTA+AGG	-	chr1_1:2131934-2131953	Msa0000820:CDS	35.0%
	GTAAGACTAAGGAAAAAGCA+AGG	-	chr1_1:2131945-2131964	Msa0000820:CDS	35.0%
	AGATACTTAAGATGCATGCT+AGG	-	chr1_1:2131969-2131988	Msa0000820:CDS	35.0%
	GATACTTAAGATGCATGCTA+GGG	-	chr1_1:2131970-2131989	Msa0000820:CDS	35.0%
	AGCAAATTACTAGGTATGCA+TGG	+	chr1_1:2132010-2132029	None:intergenic	35.0%
	TTCAATACTTTCTCTGTCGT+AGG	+	chr1_1:2130889-2130908	None:intergenic	35.0%
	TTAGAAGACTCATTCAAGCT+TGG	-	chr1_1:2130997-2131016	Msa0000820:CDS	35.0%
	TGTACATTAAACGCTTCACA+AGG	-	chr1_1:2131020-2131039	Msa0000820:CDS	35.0%
!!	GTTTTGTTGATTGGTGGTTT+TGG	+	chr1_1:2131065-2131084	None:intergenic	35.0%
!!!	ATTTGGGTTTTGTTGATTGG+TGG	+	chr1_1:2131071-2131090	None:intergenic	35.0%
!!!	GTGATTTGGGTTTTGTTGAT+TGG	+	chr1_1:2131074-2131093	None:intergenic	35.0%
!	TTTTGATGTAGTAACTGCAC+TGG	+	chr1_1:2131485-2131504	None:intergenic	35.0%
!!	AATGCTTAAACTTGGTGTTG+TGG	-	chr1_1:2131886-2131905	Msa0000820:CDS	35.0%
!!	TTGGTTCTTCAAGTTGATTG+TGG	-	chr1_1:2131920-2131939	Msa0000820:CDS	35.0%
	TGATTGTGGAAGTAAGACTA+AGG	-	chr1_1:2131934-2131953	Msa0000820:CDS	35.0%
	GTAAGACTAAGGAAAAAGCA+AGG	-	chr1_1:2131945-2131964	Msa0000820:CDS	35.0%
	AGATACTTAAGATGCATGCT+AGG	-	chr1_1:2131969-2131988	Msa0000820:CDS	35.0%
	GATACTTAAGATGCATGCTA+GGG	-	chr1_1:2131970-2131989	Msa0000820:CDS	35.0%
	AGCAAATTACTAGGTATGCA+TGG	+	chr1_1:2132010-2132029	None:intergenic	35.0%
	ATACCTGTTGAGACTGTTAC+TGG	+	chr1_1:2130864-2130883	None:intergenic	40.0%
!	AGTCTTCTAAGAGTGTGGTT+TGG	+	chr1_1:2130987-2131006	None:intergenic	40.0%
!	GAATGAGTCTTCTAAGAGTG+TGG	+	chr1_1:2130992-2131011	None:intergenic	40.0%
!!!	TTGATTGGTGGTTTTGGAGT+TGG	+	chr1_1:2131059-2131078	None:intergenic	40.0%
	AGACTTAGATCTATGGCTTC+TGG	-	chr1_1:2131152-2131171	Msa0000820:CDS	40.0%
	AAACGTTGCATAGAAGATGC+AGG	-	chr1_1:2131188-2131207	Msa0000820:CDS	40.0%
!	AATCTTCATGTGTCTGAAGC+TGG	-	chr1_1:2131329-2131348	Msa0000820:CDS	40.0%
!	TTGATTCTGTTTCTTCCCCA+TGG	+	chr1_1:2131602-2131621	None:intergenic	40.0%
	TGGGGAAGAAACAGAATCAA+AGG	-	chr1_1:2131602-2131621	Msa0000820:CDS	40.0%
!!	AGAATCAAAGGTGTTGAAGC+TGG	-	chr1_1:2131614-2131633	Msa0000820:CDS	40.0%
	AATCTTTGTCAGTGTGCTGA+AGG	-	chr1_1:2131719-2131738	Msa0000820:CDS	40.0%
	ATACCTGTTGAGACTGTTAC+TGG	+	chr1_1:2130864-2130883	None:intergenic	40.0%
!	AGTCTTCTAAGAGTGTGGTT+TGG	+	chr1_1:2130987-2131006	None:intergenic	40.0%
!	GAATGAGTCTTCTAAGAGTG+TGG	+	chr1_1:2130992-2131011	None:intergenic	40.0%
!!!	TTGATTGGTGGTTTTGGAGT+TGG	+	chr1_1:2131059-2131078	None:intergenic	40.0%
	AGACTTAGATCTATGGCTTC+TGG	-	chr1_1:2131152-2131171	Msa0000820:CDS	40.0%
	AAACGTTGCATAGAAGATGC+AGG	-	chr1_1:2131188-2131207	Msa0000820:CDS	40.0%
!	AATCTTCATGTGTCTGAAGC+TGG	-	chr1_1:2131329-2131348	Msa0000820:CDS	40.0%
!	TTGATTCTGTTTCTTCCCCA+TGG	+	chr1_1:2131602-2131621	None:intergenic	40.0%
	TGGGGAAGAAACAGAATCAA+AGG	-	chr1_1:2131602-2131621	Msa0000820:CDS	40.0%
!!	AGAATCAAAGGTGTTGAAGC+TGG	-	chr1_1:2131614-2131633	Msa0000820:CDS	40.0%
	AATCTTTGTCAGTGTGCTGA+AGG	-	chr1_1:2131719-2131738	Msa0000820:CDS	40.0%
	ATGGGGCAGACAAAGAATGA+AGG	+	chr1_1:2130819-2130838	None:intergenic	45.0%
	GATCCAGTAACAGTCTCAAC+AGG	-	chr1_1:2130858-2130877	Msa0000820:CDS	45.0%
!!	ACGCTTCACAAGGCATTGAA+AGG	-	chr1_1:2131030-2131049	Msa0000820:CDS	45.0%
	CCATGGACAGAGTGCAATTA+GGG	+	chr1_1:2131585-2131604	None:intergenic	45.0%
	CCTAATTGCACTCTGTCCAT+GGG	-	chr1_1:2131583-2131602	Msa0000820:CDS	45.0%
	CTAATTGCACTCTGTCCATG+GGG	-	chr1_1:2131584-2131603	Msa0000820:CDS	45.0%
!!!	GAACTTTTGAGACATGCAGC+AGG	-	chr1_1:2131749-2131768	Msa0000820:CDS	45.0%
	TTGCGAATGATAGAGCTGTG+AGG	-	chr1_1:2131813-2131832	Msa0000820:CDS	45.0%
	TGTTGTGGCTAAGCTTTGCT+TGG	-	chr1_1:2131901-2131920	Msa0000820:CDS	45.0%
	TTAAGATGCATGCTAGGGCA+TGG	-	chr1_1:2131975-2131994	Msa0000820:CDS	45.0%
	ATGGGGCAGACAAAGAATGA+AGG	+	chr1_1:2130819-2130838	None:intergenic	45.0%
	GATCCAGTAACAGTCTCAAC+AGG	-	chr1_1:2130858-2130877	Msa0000820:CDS	45.0%
!!	ACGCTTCACAAGGCATTGAA+AGG	-	chr1_1:2131030-2131049	Msa0000820:CDS	45.0%
	CCATGGACAGAGTGCAATTA+GGG	+	chr1_1:2131585-2131604	None:intergenic	45.0%
	CCTAATTGCACTCTGTCCAT+GGG	-	chr1_1:2131583-2131602	Msa0000820:CDS	45.0%
	CTAATTGCACTCTGTCCATG+GGG	-	chr1_1:2131584-2131603	Msa0000820:CDS	45.0%
!!!	GAACTTTTGAGACATGCAGC+AGG	-	chr1_1:2131749-2131768	Msa0000820:CDS	45.0%
	TTGCGAATGATAGAGCTGTG+AGG	-	chr1_1:2131813-2131832	Msa0000820:CDS	45.0%
	TGTTGTGGCTAAGCTTTGCT+TGG	-	chr1_1:2131901-2131920	Msa0000820:CDS	45.0%
	TTAAGATGCATGCTAGGGCA+TGG	-	chr1_1:2131975-2131994	Msa0000820:CDS	45.0%
	CCCTAATTGCACTCTGTCCA+TGG	-	chr1_1:2131582-2131601	Msa0000820:CDS	50.0%
	CCCATGGACAGAGTGCAATT+AGG	+	chr1_1:2131586-2131605	None:intergenic	50.0%
	CCCTAATTGCACTCTGTCCA+TGG	-	chr1_1:2131582-2131601	Msa0000820:CDS	50.0%
	CCCATGGACAGAGTGCAATT+AGG	+	chr1_1:2131586-2131605	None:intergenic	50.0%
	AGATGCATGCTAGGGCATGG+AGG	-	chr1_1:2131978-2131997	Msa0000820:CDS	55.0%
	AGATGCATGCTAGGGCATGG+AGG	-	chr1_1:2131978-2131997	Msa0000820:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	2130792	2132054	2130792	ID=Msa0000820;Name=Msa0000820
chr1_1	mRNA	2130792	2132054	2130792	ID=Msa0000820-mRNA-1;Parent=Msa0000820;Name=Msa0000820-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|420
chr1_1	exon	2130792	2132054	2130792	ID=Msa0000820-mRNA-1:exon:407;Parent=Msa0000820-mRNA-1
chr1_1	CDS	2130792	2132054	2130792	ID=Msa0000820-mRNA-1:cds;Parent=Msa0000820-mRNA-1

Gene Sequence

>Msa0000820

ATGGATCATGATGAGATCGAGGTTCCTTCATTCTTTGTCTGCCCCATATCATTAGAAATCATGAAGGATCCAGTAACAGTCTCAACAGGTATAACCTACGACAGAGAAAGTATTGAAAAATGGCTATTTTCAAAGAAAAACATGACATGTCCTATTACAAAACAACAGCTATCAGATTACACTGATCTAACACCAAACCACACTCTTAGAAGACTCATTCAAGCTTGGTGTACATTAAACGCTTCACAAGGCATTGAAAGGATTCCAACTCCAAAACCACCAATCAACAAAACCCAAATCACAAAACTCATCAAAGAAGCGTCTCATTCATCTCTCACTGTGCAGCTTAAATGCTTGAAAAGACTTAGATCTATGGCTTCTGGAAGTGAAACAAACAAACGTTGCATAGAAGATGCAGGTGCTGTTGAGTTTATAGCTTCTATTGTAATAAACAATAGTTGCAACATTGACTTTGTTAACAACTCTGGATCTTTATCAGAGACTAACACTGTTGATGAAGCATTAAGCATACTTCATAATCTTCATGTGTCTGAAGCTGGATTAAAGAAACTACTTGCTTTCAGAAACGGCGAATTTATTGAATCCTTAACAAAAGTTATGCAGAAAGGTTTCTTTGAGTCAAGAGCTTATGCAGTTTTCTTATTAAAATCTATGTCAGAAGTAGCTGAACCAGTGCAGTTACTACATCAAAAAATTGAGATGTTCATTGAATTAGTACAAGTTTTGAAGGATCAAATATCACAGAAAGTATCAAAAGCAGCACTTCAGACCCTAATTGCACTCTGTCCATGGGGAAGAAACAGAATCAAAGGTGTTGAAGCTGGAACAGTTCCTGTTTTAATAGAACTTTTGTTAGAAAATTGCAAAGACAGAAAACCAAATGAAATGATGTTGGTTTTGTTGGAGAATCTTTGTCAGTGTGCTGAAGGAAGAGCTGAACTTTTGAGACATGCAGCAGGTCTTGCTGTTGTTTCTAAGAAGATTCTAAGGGTTTCGACGCTTGCGAATGATAGAGCTGTGAGGATTCTGTTGTCTGTTTCAAGATTCTCTGCAACTCCTAATGTTGTTCAAGAAATGCTTAAACTTGGTGTTGTGGCTAAGCTTTGCTTGGTTCTTCAAGTTGATTGTGGAAGTAAGACTAAGGAAAAAGCAAGGGAGATACTTAAGATGCATGCTAGGGCATGGAGGAATTCTCCATGCATACCTAGTAATTTGCTTTCTTCATACCCCAATTACCAGTGA

Protein sequence

>Msa0000820

MDHDEIEVPSFFVCPISLEIMKDPVTVSTGITYDRESIEKWLFSKKNMTCPITKQQLSDYTDLTPNHTLRRLIQAWCTLNASQGIERIPTPKPPINKTQITKLIKEASHSSLTVQLKCLKRLRSMASGSETNKRCIEDAGAVEFIASIVINNSCNIDFVNNSGSLSETNTVDEALSILHNLHVSEAGLKKLLAFRNGEFIESLTKVMQKGFFESRAYAVFLLKSMSEVAEPVQLLHQKIEMFIELVQVLKDQISQKVSKAALQTLIALCPWGRNRIKGVEAGTVPVLIELLLENCKDRKPNEMMLVLLENLCQCAEGRAELLRHAAGLAVVSKKILRVSTLANDRAVRILLSVSRFSATPNVVQEMLKLGVVAKLCLVLQVDCGSKTKEKAREILKMHARAWRNSPCIPSNLLSSYPNYQ*