AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0897020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAACAGATTGAGATTT+TGG 0.155235 6_1:-88296560 Msa0897020:CDS
AACCCTTCCTTCATCTTCTT+TGG 0.218501 6_1:+88296152 None:intergenic
CCTCTATAGTCTAAGTGTTT+TGG 0.250918 6_1:+88297843 None:intergenic
CCTCAAAGGAATGACTAATT+AGG 0.261604 6_1:+88296892 None:intergenic
TGTTAAGAATGCTTTGGATT+TGG 0.295341 6_1:-88296207 Msa0897020:CDS
CCTAATTAGTCATTCCTTTG+AGG 0.320162 6_1:-88296892 Msa0897020:CDS
GGATAACAATCTTTCATCAT+TGG 0.397150 6_1:-88296835 Msa0897020:intron
TTGCAGTGTTAAGAATGCTT+TGG 0.416508 6_1:-88296213 Msa0897020:intron
CAACGTAGATCAAACTGGTT+TGG 0.417119 6_1:-88296865 Msa0897020:CDS
AAATCCTGTCCAATAATAGA+AGG 0.445537 6_1:+88295975 None:intergenic
TCTGTACTGCAAATGATTGT+TGG 0.454375 6_1:-88296103 Msa0897020:three_prime_UTR
TCAAACTGGTTTGGATGAAT+CGG 0.469296 6_1:-88296856 Msa0897020:CDS
CCAAAACACTTAGACTATAG+AGG 0.473947 6_1:-88297843 Msa0897020:five_prime_UTR
ACCACCTTTACAAGCTAGTC+TGG 0.480028 6_1:+88296526 None:intergenic
ATCTACGTTGGTTTCCTCAA+AGG 0.502573 6_1:+88296878 None:intergenic
GAAACCAACGTAGATCAAAC+TGG 0.504773 6_1:-88296870 Msa0897020:CDS
CAGAGGTATAGAAATTGAAA+AGG 0.521398 6_1:-88297695 Msa0897020:five_prime_UTR
CAAATGATTGTTGGTAGCAA+GGG 0.542096 6_1:-88296094 Msa0897020:three_prime_UTR
GCAAATGATTGTTGGTAGCA+AGG 0.555490 6_1:-88296095 Msa0897020:three_prime_UTR
TGAATGTATAAAGTCCGAAT+CGG 0.569659 6_1:+88296011 None:intergenic
TGACCAAAGAAGATGAAGGA+AGG 0.569743 6_1:-88296155 Msa0897020:three_prime_UTR
CATTTGCGCTAGATGAGCAT+AGG 0.571587 6_1:+88297732 None:intergenic
CAAACCAGTTTGATCTACGT+TGG 0.575664 6_1:+88296866 None:intergenic
AGGTATAGAAATTGAAAAGG+TGG 0.588046 6_1:-88297692 Msa0897020:five_prime_UTR
TTCTACAACTTCTAATACCG+AGG 0.590215 6_1:+88297645 None:intergenic
GACCAAAGAAGATGAAGGAA+GGG 0.592744 6_1:-88296154 Msa0897020:three_prime_UTR
AATATGACCAAAGAAGATGA+AGG 0.618106 6_1:-88296159 Msa0897020:three_prime_UTR
AAATTAACATATCATCAATG+AGG 0.643337 6_1:+88297595 None:intergenic
TGTTGATAAGAAGATTACCT+CGG 0.656249 6_1:-88297662 Msa0897020:five_prime_UTR
TTTCTTTAATATGCACCCTG+AGG 0.665853 6_1:-88297564 Msa0897020:intron
ATGAAGTATATACTTCCCAG+AGG 0.760682 6_1:-88297712 Msa0897020:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATCTATTACTGTTATTTTT+TGG - chr6_1:88296484-88296503 Msa0897020:intron 15.0%
!!! ATCTATTACTGTTATTTTTT+GGG - chr6_1:88296485-88296504 Msa0897020:intron 15.0%
!!! TATCTATTACTGTTATTTTT+TGG - chr6_1:88296484-88296503 Msa0897020:intron 15.0%
!!! ATCTATTACTGTTATTTTTT+GGG - chr6_1:88296485-88296504 Msa0897020:intron 15.0%
!! AAATTAACATATCATCAATG+AGG + chr6_1:88296206-88296225 None:intergenic 20.0%
!!! ATTGTATTTTCTGTATTTGA+CGG - chr6_1:88296293-88296312 Msa0897020:intron 20.0%
!!! TAGTATTACATTGATTATGT+TGG + chr6_1:88296466-88296485 None:intergenic 20.0%
!!! TGAATTTATGTTTGCTATAA+TGG - chr6_1:88297403-88297422 Msa0897020:intron 20.0%
!!! TTAAGTTTTCCTTCTATTAT+TGG - chr6_1:88297814-88297833 Msa0897020:five_prime_UTR 20.0%
!! AAATTAACATATCATCAATG+AGG + chr6_1:88296206-88296225 None:intergenic 20.0%
!!! ATTGTATTTTCTGTATTTGA+CGG - chr6_1:88296293-88296312 Msa0897020:intron 20.0%
!!! TAGTATTACATTGATTATGT+TGG + chr6_1:88296466-88296485 None:intergenic 20.0%
!!! TGAATTTATGTTTGCTATAA+TGG - chr6_1:88297403-88297422 Msa0897020:intron 20.0%
!!! TTAAGTTTTCCTTCTATTAT+TGG - chr6_1:88297814-88297833 Msa0897020:five_prime_UTR 20.0%
!!! AGTGTTTGTTTTTGGAATAA+AGG - chr6_1:88296016-88296035 Msa0897020:three_prime_UTR 25.0%
!! TTTTCAATTTCTATACCTCT+GGG + chr6_1:88296104-88296123 None:intergenic 25.0%
! CTTAAAAAAATGTTACCTCA+GGG + chr6_1:88296252-88296271 None:intergenic 25.0%
!!! CTGAGGTAACATTTTTTTAA+GGG - chr6_1:88296251-88296270 Msa0897020:intron 25.0%
! AATTAATGCTATCTTAACAG+TGG - chr6_1:88296319-88296338 Msa0897020:intron 25.0%
!! TATTTTATATGCTAAGAGTC+CGG - chr6_1:88296384-88296403 Msa0897020:intron 25.0%
! GAATTAAAACCAAAACAAGA+TGG + chr6_1:88296737-88296756 None:intergenic 25.0%
!!! TCTTGTTTTGGTTTTAATTC+TGG - chr6_1:88296737-88296756 Msa0897020:intron 25.0%
! GTAATTGCTTTCTTGTTAAT+TGG - chr6_1:88296859-88296878 Msa0897020:CDS 25.0%
!!! TACTTTGAAATAAATGACAG+CGG + chr6_1:88297504-88297523 None:intergenic 25.0%
!!! AGTGTTTGTTTTTGGAATAA+AGG - chr6_1:88296016-88296035 Msa0897020:three_prime_UTR 25.0%
!! TTTTCAATTTCTATACCTCT+GGG + chr6_1:88296104-88296123 None:intergenic 25.0%
! CTTAAAAAAATGTTACCTCA+GGG + chr6_1:88296252-88296271 None:intergenic 25.0%
!!! CTGAGGTAACATTTTTTTAA+GGG - chr6_1:88296251-88296270 Msa0897020:intron 25.0%
! AATTAATGCTATCTTAACAG+TGG - chr6_1:88296319-88296338 Msa0897020:intron 25.0%
!! TATTTTATATGCTAAGAGTC+CGG - chr6_1:88296384-88296403 Msa0897020:intron 25.0%
! GAATTAAAACCAAAACAAGA+TGG + chr6_1:88296737-88296756 None:intergenic 25.0%
!!! TCTTGTTTTGGTTTTAATTC+TGG - chr6_1:88296737-88296756 Msa0897020:intron 25.0%
! GTAATTGCTTTCTTGTTAAT+TGG - chr6_1:88296859-88296878 Msa0897020:CDS 25.0%
!!! TACTTTGAAATAAATGACAG+CGG + chr6_1:88297504-88297523 None:intergenic 25.0%
! CTTTTCAATTTCTATACCTC+TGG + chr6_1:88296105-88296124 None:intergenic 30.0%
! CAGAGGTATAGAAATTGAAA+AGG - chr6_1:88296103-88296122 Msa0897020:three_prime_UTR 30.0%
AGGTATAGAAATTGAAAAGG+TGG - chr6_1:88296106-88296125 Msa0897020:three_prime_UTR 30.0%
! TGTTGATAAGAAGATTACCT+CGG - chr6_1:88296136-88296155 Msa0897020:three_prime_UTR 30.0%
CCTTAAAAAAATGTTACCTC+AGG + chr6_1:88296253-88296272 None:intergenic 30.0%
!!! CCTGAGGTAACATTTTTTTA+AGG - chr6_1:88296250-88296269 Msa0897020:intron 30.0%
ATTCAAGAATGCTACTGTAA+TGG - chr6_1:88296530-88296549 Msa0897020:intron 30.0%
TTGTGATTAGAGATTCCATA+TGG - chr6_1:88296588-88296607 Msa0897020:intron 30.0%
AGAAAGTAGTATACGAGATT+TGG - chr6_1:88296662-88296681 Msa0897020:intron 30.0%
GGATAACAATCTTTCATCAT+TGG - chr6_1:88296963-88296982 Msa0897020:intron 30.0%
GGTGTGATTAACTCTATATT+TGG - chr6_1:88296984-88297003 Msa0897020:intron 30.0%
! TGAAGAACAGATTGAGATTT+TGG - chr6_1:88297095-88297114 Msa0897020:intron 30.0%
!!! CCTTTGTTCTTTTAGAGTAT+CGG - chr6_1:88297201-88297220 Msa0897020:intron 30.0%
! TGAAGAACAGATTGAGATTT+TGG - chr6_1:88297095-88297114 Msa0897020:intron 30.0%
!!! CCTTTGTTCTTTTAGAGTAT+CGG - chr6_1:88297201-88297220 Msa0897020:intron 30.0%
!! AAATAAATGACAGCGGATTT+TGG + chr6_1:88297497-88297516 None:intergenic 30.0%
! TGTTAAGAATGCTTTGGATT+TGG - chr6_1:88297591-88297610 Msa0897020:five_prime_UTR 30.0%
AATATGACCAAAGAAGATGA+AGG - chr6_1:88297639-88297658 Msa0897020:five_prime_UTR 30.0%
!! AATTTTGAGTAACACCGATT+CGG - chr6_1:88297773-88297792 Msa0897020:five_prime_UTR 30.0%
TGAATGTATAAAGTCCGAAT+CGG + chr6_1:88297790-88297809 None:intergenic 30.0%
! TTTTCCTTCTATTATTGGAC+AGG - chr6_1:88297819-88297838 Msa0897020:five_prime_UTR 30.0%
AAATCCTGTCCAATAATAGA+AGG + chr6_1:88297826-88297845 None:intergenic 30.0%
! CTTTTCAATTTCTATACCTC+TGG + chr6_1:88296105-88296124 None:intergenic 30.0%
! CAGAGGTATAGAAATTGAAA+AGG - chr6_1:88296103-88296122 Msa0897020:three_prime_UTR 30.0%
AGGTATAGAAATTGAAAAGG+TGG - chr6_1:88296106-88296125 Msa0897020:three_prime_UTR 30.0%
! TGTTGATAAGAAGATTACCT+CGG - chr6_1:88296136-88296155 Msa0897020:three_prime_UTR 30.0%
CCTTAAAAAAATGTTACCTC+AGG + chr6_1:88296253-88296272 None:intergenic 30.0%
!!! CCTGAGGTAACATTTTTTTA+AGG - chr6_1:88296250-88296269 Msa0897020:intron 30.0%
ATTCAAGAATGCTACTGTAA+TGG - chr6_1:88296530-88296549 Msa0897020:intron 30.0%
TTGTGATTAGAGATTCCATA+TGG - chr6_1:88296588-88296607 Msa0897020:intron 30.0%
AGAAAGTAGTATACGAGATT+TGG - chr6_1:88296662-88296681 Msa0897020:intron 30.0%
GGATAACAATCTTTCATCAT+TGG - chr6_1:88296963-88296982 Msa0897020:intron 30.0%
GGTGTGATTAACTCTATATT+TGG - chr6_1:88296984-88297003 Msa0897020:intron 30.0%
! TGAAGAACAGATTGAGATTT+TGG - chr6_1:88297095-88297114 Msa0897020:intron 30.0%
!!! CCTTTGTTCTTTTAGAGTAT+CGG - chr6_1:88297201-88297220 Msa0897020:intron 30.0%
! TGAAGAACAGATTGAGATTT+TGG - chr6_1:88297095-88297114 Msa0897020:intron 30.0%
!!! CCTTTGTTCTTTTAGAGTAT+CGG - chr6_1:88297201-88297220 Msa0897020:intron 30.0%
!! AAATAAATGACAGCGGATTT+TGG + chr6_1:88297497-88297516 None:intergenic 30.0%
! TGTTAAGAATGCTTTGGATT+TGG - chr6_1:88297591-88297610 Msa0897020:five_prime_UTR 30.0%
AATATGACCAAAGAAGATGA+AGG - chr6_1:88297639-88297658 Msa0897020:five_prime_UTR 30.0%
!! AATTTTGAGTAACACCGATT+CGG - chr6_1:88297773-88297792 Msa0897020:five_prime_UTR 30.0%
TGAATGTATAAAGTCCGAAT+CGG + chr6_1:88297790-88297809 None:intergenic 30.0%
! TTTTCCTTCTATTATTGGAC+AGG - chr6_1:88297819-88297838 Msa0897020:five_prime_UTR 30.0%
AAATCCTGTCCAATAATAGA+AGG + chr6_1:88297826-88297845 None:intergenic 30.0%
! CCTCTATAGTCTAAGTGTTT+TGG + chr6_1:88295958-88295977 None:intergenic 35.0%
CCAAAACACTTAGACTATAG+AGG - chr6_1:88295955-88295974 Msa0897020:three_prime_UTR 35.0%
!! AGGACTGTAGTGTTTGTTTT+TGG - chr6_1:88296008-88296027 Msa0897020:three_prime_UTR 35.0%
ATGAAGTATATACTTCCCAG+AGG - chr6_1:88296086-88296105 Msa0897020:three_prime_UTR 35.0%
TTCTACAACTTCTAATACCG+AGG + chr6_1:88296156-88296175 None:intergenic 35.0%
TTTCTTTAATATGCACCCTG+AGG - chr6_1:88296234-88296253 Msa0897020:intron 35.0%
CTATCTTAACAGTGGTGTAT+GGG - chr6_1:88296327-88296346 Msa0897020:intron 35.0%
TCAGACTAGATTTCGAATTC+CGG + chr6_1:88296406-88296425 None:intergenic 35.0%
GAATTCGAAATCTAGTCTGA+GGG - chr6_1:88296406-88296425 Msa0897020:intron 35.0%
!! AGGGATTTCTGAAACTTCAT+TGG - chr6_1:88296425-88296444 Msa0897020:intron 35.0%
!!! ATTTTTTGGGAACATAGTCG+AGG - chr6_1:88296498-88296517 Msa0897020:intron 35.0%
!!! TTTTTTGGGAACATAGTCGA+GGG - chr6_1:88296499-88296518 Msa0897020:intron 35.0%
GCTTACATCTACTTACCATA+TGG + chr6_1:88296606-88296625 None:intergenic 35.0%
!! ATTTAGCTGCCATCTTGTTT+TGG - chr6_1:88296725-88296744 Msa0897020:intron 35.0%
CCTCAAAGGAATGACTAATT+AGG + chr6_1:88296909-88296928 None:intergenic 35.0%
CCTAATTAGTCATTCCTTTG+AGG - chr6_1:88296906-88296925 Msa0897020:CDS 35.0%
!! TCAAACTGGTTTGGATGAAT+CGG - chr6_1:88296942-88296961 Msa0897020:intron 35.0%
CTAATCTTATTCCTCCCAAT+AGG - chr6_1:88297066-88297085 Msa0897020:intron 35.0%
! CCGATACTCTAAAAGAACAA+AGG + chr6_1:88297204-88297223 None:intergenic 35.0%
! CCGATACTCTAAAAGAACAA+AGG + chr6_1:88297204-88297223 None:intergenic 35.0%
!!! TTAGAGTATCGGTCTCTTTT+AGG - chr6_1:88297212-88297231 Msa0897020:intron 35.0%
!!! TAGAGTATCGGTCTCTTTTA+GGG - chr6_1:88297213-88297232 Msa0897020:intron 35.0%
TTGCAGTGTTAAGAATGCTT+TGG - chr6_1:88297585-88297604 Msa0897020:five_prime_UTR 35.0%
TCTGTACTGCAAATGATTGT+TGG - chr6_1:88297695-88297714 Msa0897020:five_prime_UTR 35.0%
!! CAAATGATTGTTGGTAGCAA+GGG - chr6_1:88297704-88297723 Msa0897020:five_prime_UTR 35.0%
! CCTCTATAGTCTAAGTGTTT+TGG + chr6_1:88295958-88295977 None:intergenic 35.0%
CCAAAACACTTAGACTATAG+AGG - chr6_1:88295955-88295974 Msa0897020:three_prime_UTR 35.0%
!! AGGACTGTAGTGTTTGTTTT+TGG - chr6_1:88296008-88296027 Msa0897020:three_prime_UTR 35.0%
ATGAAGTATATACTTCCCAG+AGG - chr6_1:88296086-88296105 Msa0897020:three_prime_UTR 35.0%
TTCTACAACTTCTAATACCG+AGG + chr6_1:88296156-88296175 None:intergenic 35.0%
TTTCTTTAATATGCACCCTG+AGG - chr6_1:88296234-88296253 Msa0897020:intron 35.0%
CTATCTTAACAGTGGTGTAT+GGG - chr6_1:88296327-88296346 Msa0897020:intron 35.0%
TCAGACTAGATTTCGAATTC+CGG + chr6_1:88296406-88296425 None:intergenic 35.0%
GAATTCGAAATCTAGTCTGA+GGG - chr6_1:88296406-88296425 Msa0897020:intron 35.0%
!! AGGGATTTCTGAAACTTCAT+TGG - chr6_1:88296425-88296444 Msa0897020:intron 35.0%
!!! ATTTTTTGGGAACATAGTCG+AGG - chr6_1:88296498-88296517 Msa0897020:intron 35.0%
!!! TTTTTTGGGAACATAGTCGA+GGG - chr6_1:88296499-88296518 Msa0897020:intron 35.0%
GCTTACATCTACTTACCATA+TGG + chr6_1:88296606-88296625 None:intergenic 35.0%
!! ATTTAGCTGCCATCTTGTTT+TGG - chr6_1:88296725-88296744 Msa0897020:intron 35.0%
CCTCAAAGGAATGACTAATT+AGG + chr6_1:88296909-88296928 None:intergenic 35.0%
CCTAATTAGTCATTCCTTTG+AGG - chr6_1:88296906-88296925 Msa0897020:CDS 35.0%
!! TCAAACTGGTTTGGATGAAT+CGG - chr6_1:88296942-88296961 Msa0897020:intron 35.0%
CTAATCTTATTCCTCCCAAT+AGG - chr6_1:88297066-88297085 Msa0897020:intron 35.0%
! CCGATACTCTAAAAGAACAA+AGG + chr6_1:88297204-88297223 None:intergenic 35.0%
! CCGATACTCTAAAAGAACAA+AGG + chr6_1:88297204-88297223 None:intergenic 35.0%
!!! TTAGAGTATCGGTCTCTTTT+AGG - chr6_1:88297212-88297231 Msa0897020:intron 35.0%
!!! TAGAGTATCGGTCTCTTTTA+GGG - chr6_1:88297213-88297232 Msa0897020:intron 35.0%
TTGCAGTGTTAAGAATGCTT+TGG - chr6_1:88297585-88297604 Msa0897020:five_prime_UTR 35.0%
TCTGTACTGCAAATGATTGT+TGG - chr6_1:88297695-88297714 Msa0897020:five_prime_UTR 35.0%
!! CAAATGATTGTTGGTAGCAA+GGG - chr6_1:88297704-88297723 Msa0897020:five_prime_UTR 35.0%
GCTATCTTAACAGTGGTGTA+TGG - chr6_1:88296326-88296345 Msa0897020:intron 40.0%
GTGGTGTATGGGATCTTATT+CGG - chr6_1:88296338-88296357 Msa0897020:intron 40.0%
GGAATTCGAAATCTAGTCTG+AGG - chr6_1:88296405-88296424 Msa0897020:intron 40.0%
!! ATCTACGTTGGTTTCCTCAA+AGG + chr6_1:88296923-88296942 None:intergenic 40.0%
GAAACCAACGTAGATCAAAC+TGG - chr6_1:88296928-88296947 Msa0897020:intron 40.0%
CAAACCAGTTTGATCTACGT+TGG + chr6_1:88296935-88296954 None:intergenic 40.0%
CAACGTAGATCAAACTGGTT+TGG - chr6_1:88296933-88296952 Msa0897020:intron 40.0%
GTTCTTCAGCTTCTCCTATT+GGG + chr6_1:88297083-88297102 None:intergenic 40.0%
TGTTCTTCAGCTTCTCCTAT+TGG + chr6_1:88297084-88297103 None:intergenic 40.0%
CTTGTAAAGGTGGTTTCTTC+TGG - chr6_1:88297138-88297157 Msa0897020:intron 40.0%
CTTGTAAAGGTGGTTTCTTC+TGG - chr6_1:88297138-88297157 Msa0897020:intron 40.0%
TGACCAAAGAAGATGAAGGA+AGG - chr6_1:88297643-88297662 Msa0897020:five_prime_UTR 40.0%
GACCAAAGAAGATGAAGGAA+GGG - chr6_1:88297644-88297663 Msa0897020:five_prime_UTR 40.0%
AACCCTTCCTTCATCTTCTT+TGG + chr6_1:88297649-88297668 None:intergenic 40.0%
!! GCAAATGATTGTTGGTAGCA+AGG - chr6_1:88297703-88297722 Msa0897020:five_prime_UTR 40.0%
GCTATCTTAACAGTGGTGTA+TGG - chr6_1:88296326-88296345 Msa0897020:intron 40.0%
GTGGTGTATGGGATCTTATT+CGG - chr6_1:88296338-88296357 Msa0897020:intron 40.0%
GGAATTCGAAATCTAGTCTG+AGG - chr6_1:88296405-88296424 Msa0897020:intron 40.0%
!! ATCTACGTTGGTTTCCTCAA+AGG + chr6_1:88296923-88296942 None:intergenic 40.0%
GAAACCAACGTAGATCAAAC+TGG - chr6_1:88296928-88296947 Msa0897020:intron 40.0%
CAAACCAGTTTGATCTACGT+TGG + chr6_1:88296935-88296954 None:intergenic 40.0%
CAACGTAGATCAAACTGGTT+TGG - chr6_1:88296933-88296952 Msa0897020:intron 40.0%
GTTCTTCAGCTTCTCCTATT+GGG + chr6_1:88297083-88297102 None:intergenic 40.0%
TGTTCTTCAGCTTCTCCTAT+TGG + chr6_1:88297084-88297103 None:intergenic 40.0%
CTTGTAAAGGTGGTTTCTTC+TGG - chr6_1:88297138-88297157 Msa0897020:intron 40.0%
CTTGTAAAGGTGGTTTCTTC+TGG - chr6_1:88297138-88297157 Msa0897020:intron 40.0%
TGACCAAAGAAGATGAAGGA+AGG - chr6_1:88297643-88297662 Msa0897020:five_prime_UTR 40.0%
GACCAAAGAAGATGAAGGAA+GGG - chr6_1:88297644-88297663 Msa0897020:five_prime_UTR 40.0%
AACCCTTCCTTCATCTTCTT+TGG + chr6_1:88297649-88297668 None:intergenic 40.0%
!! GCAAATGATTGTTGGTAGCA+AGG - chr6_1:88297703-88297722 Msa0897020:five_prime_UTR 40.0%
CATTTGCGCTAGATGAGCAT+AGG + chr6_1:88296069-88296088 None:intergenic 45.0%
GGAACATAGTCGAGGGATTA+TGG - chr6_1:88296506-88296525 Msa0897020:intron 45.0%
!! TGTGCCAGACTAGCTTGTAA+AGG - chr6_1:88297125-88297144 Msa0897020:intron 45.0%
ACCACCTTTACAAGCTAGTC+TGG + chr6_1:88297132-88297151 None:intergenic 45.0%
! CTTCTGGAGACACTGTGTAA+TGG - chr6_1:88297154-88297173 Msa0897020:intron 45.0%
TGGAGACACTGTGTAATGGT+GGG - chr6_1:88297158-88297177 Msa0897020:intron 45.0%
!! TGTGCCAGACTAGCTTGTAA+AGG - chr6_1:88297125-88297144 Msa0897020:intron 45.0%
ACCACCTTTACAAGCTAGTC+TGG + chr6_1:88297132-88297151 None:intergenic 45.0%
! CTTCTGGAGACACTGTGTAA+TGG - chr6_1:88297154-88297173 Msa0897020:intron 45.0%
TGGAGACACTGTGTAATGGT+GGG - chr6_1:88297158-88297177 Msa0897020:intron 45.0%
CATTTGCGCTAGATGAGCAT+AGG + chr6_1:88296069-88296088 None:intergenic 45.0%
GGAACATAGTCGAGGGATTA+TGG - chr6_1:88296506-88296525 Msa0897020:intron 45.0%
!! TGTGCCAGACTAGCTTGTAA+AGG - chr6_1:88297125-88297144 Msa0897020:intron 45.0%
ACCACCTTTACAAGCTAGTC+TGG + chr6_1:88297132-88297151 None:intergenic 45.0%
! CTTCTGGAGACACTGTGTAA+TGG - chr6_1:88297154-88297173 Msa0897020:intron 45.0%
TGGAGACACTGTGTAATGGT+GGG - chr6_1:88297158-88297177 Msa0897020:intron 45.0%
!! TGTGCCAGACTAGCTTGTAA+AGG - chr6_1:88297125-88297144 Msa0897020:intron 45.0%
ACCACCTTTACAAGCTAGTC+TGG + chr6_1:88297132-88297151 None:intergenic 45.0%
! CTTCTGGAGACACTGTGTAA+TGG - chr6_1:88297154-88297173 Msa0897020:intron 45.0%
TGGAGACACTGTGTAATGGT+GGG - chr6_1:88297158-88297177 Msa0897020:intron 45.0%
!! AATTTATTATTAAAAAGAAA+TGG + chr6_1:88295983-88296002 None:intergenic 5.0%
!!! TTTTAATAATAAATTAAGAA+AGG - chr6_1:88295988-88296007 Msa0897020:three_prime_UTR 5.0%
!! AATTTATTATTAAAAAGAAA+TGG + chr6_1:88295983-88296002 None:intergenic 5.0%
!!! TTTTAATAATAAATTAAGAA+AGG - chr6_1:88295988-88296007 Msa0897020:three_prime_UTR 5.0%
CTTCAGCTTCTCCTATTGGG+AGG + chr6_1:88297080-88297099 None:intergenic 50.0%
!! GCCAGACTAGCTTGTAAAGG+TGG - chr6_1:88297128-88297147 Msa0897020:intron 50.0%
CTGGAGACACTGTGTAATGG+TGG - chr6_1:88297157-88297176 Msa0897020:intron 50.0%
!! GCCAGACTAGCTTGTAAAGG+TGG - chr6_1:88297128-88297147 Msa0897020:intron 50.0%
CTGGAGACACTGTGTAATGG+TGG - chr6_1:88297157-88297176 Msa0897020:intron 50.0%
CTTCAGCTTCTCCTATTGGG+AGG + chr6_1:88297080-88297099 None:intergenic 50.0%
!! GCCAGACTAGCTTGTAAAGG+TGG - chr6_1:88297128-88297147 Msa0897020:intron 50.0%
CTGGAGACACTGTGTAATGG+TGG - chr6_1:88297157-88297176 Msa0897020:intron 50.0%
!! GCCAGACTAGCTTGTAAAGG+TGG - chr6_1:88297128-88297147 Msa0897020:intron 50.0%
CTGGAGACACTGTGTAATGG+TGG - chr6_1:88297157-88297176 Msa0897020:intron 50.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 88295949 88297871 88295949 ID=Msa0897020;Name=Msa0897020
chr6_1 mRNA 88295949 88297871 88295949 ID=Msa0897020-mRNA-1;Parent=Msa0897020;Name=Msa0897020-mRNA-1;_AED=0.16;_eAED=0.22;_QI=295|0.33|0.5|1|0|0|4|244|62
chr6_1 exon 88295949 88296229 88295949 ID=Msa0897020-mRNA-1:exon:14981;Parent=Msa0897020-mRNA-1
chr6_1 exon 88296537 88296586 88296537 ID=Msa0897020-mRNA-1:exon:14980;Parent=Msa0897020-mRNA-1
chr6_1 exon 88296836 88296925 88296836 ID=Msa0897020-mRNA-1:exon:14979;Parent=Msa0897020-mRNA-1
chr6_1 exon 88297565 88297871 88297565 ID=Msa0897020-mRNA-1:exon:14978;Parent=Msa0897020-mRNA-1
chr6_1 five_prime_UTR 88297577 88297871 88297577 ID=Msa0897020-mRNA-1:five_prime_utr;Parent=Msa0897020-mRNA-1
chr6_1 CDS 88297565 88297576 88297565 ID=Msa0897020-mRNA-1:cds;Parent=Msa0897020-mRNA-1
chr6_1 CDS 88296836 88296925 88296836 ID=Msa0897020-mRNA-1:cds;Parent=Msa0897020-mRNA-1
chr6_1 CDS 88296537 88296586 88296537 ID=Msa0897020-mRNA-1:cds;Parent=Msa0897020-mRNA-1
chr6_1 CDS 88296193 88296229 88296193 ID=Msa0897020-mRNA-1:cds;Parent=Msa0897020-mRNA-1
chr6_1 three_prime_UTR 88295949 88296192 88295949 ID=Msa0897020-mRNA-1:three_prime_utr;Parent=Msa0897020-mRNA-1
Gene Sequence

>Msa0897020

ATGCACCCTGAGTTAGAAGAAAGCCTAATTAGTCATTCCTTTGAGGAAACCAACGTAGATCAAACTGGTTTGGATGAATCGGATAACAATCTTTCATCATTGAAGCTGAAGAACAGATTGAGATTTTGGAAAAGATTGTGCCAGACTAGCTTTGTTAAGAATGCTTTGGATTTGGACTCAGTCGACTAA
Protein sequence

>Msa0897020

MHPELEESLISHSFEETNVDQTGLDESDNNLSSLKLKNRLRFWKRLCQTSFVKNALDLDSVD*