AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0896040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAACAGATTGAGATTT+TGG 0.155235 6_1:-85503503 Msa0896040:CDS
AACCCTTCCTTCATCTTCTT+TGG 0.218501 6_1:+85503095 None:intergenic
CCTCTATAGTCTAAGTGTTT+TGG 0.250918 6_1:+85504786 None:intergenic
CCTCAAAGGAATGACTAATT+AGG 0.261604 6_1:+85503835 None:intergenic
TGTTAAGAATGCTTTGGATT+TGG 0.295341 6_1:-85503150 Msa0896040:CDS
CCTAATTAGTCATTCCTTTG+AGG 0.320162 6_1:-85503835 Msa0896040:CDS
GGATAACAATCTTTCATCAT+TGG 0.397150 6_1:-85503778 Msa0896040:intron
TTGCAGTGTTAAGAATGCTT+TGG 0.416508 6_1:-85503156 Msa0896040:intron
CAACGTAGATCAAACTGGTT+TGG 0.417119 6_1:-85503808 Msa0896040:CDS
AAATCCTGTCCAATAATAGA+AGG 0.445537 6_1:+85502918 None:intergenic
TCTGTACTGCAAATGATTGT+TGG 0.454375 6_1:-85503046 Msa0896040:three_prime_UTR
TCAAACTGGTTTGGATGAAT+CGG 0.469296 6_1:-85503799 Msa0896040:CDS
CCAAAACACTTAGACTATAG+AGG 0.473947 6_1:-85504786 Msa0896040:five_prime_UTR
ACCACCTTTACAAGCTAGTC+TGG 0.480028 6_1:+85503469 None:intergenic
ATCTACGTTGGTTTCCTCAA+AGG 0.502573 6_1:+85503821 None:intergenic
GAAACCAACGTAGATCAAAC+TGG 0.504773 6_1:-85503813 Msa0896040:CDS
CAGAGGTATAGAAATTGAAA+AGG 0.521398 6_1:-85504638 Msa0896040:five_prime_UTR
CAAATGATTGTTGGTAGCAA+GGG 0.542096 6_1:-85503037 Msa0896040:three_prime_UTR
GCAAATGATTGTTGGTAGCA+AGG 0.555490 6_1:-85503038 Msa0896040:three_prime_UTR
TGAATGTATAAAGTCCGAAT+CGG 0.569659 6_1:+85502954 None:intergenic
TGACCAAAGAAGATGAAGGA+AGG 0.569743 6_1:-85503098 Msa0896040:three_prime_UTR
CATTTGCGCTAGATGAGCAT+AGG 0.571587 6_1:+85504675 None:intergenic
CAAACCAGTTTGATCTACGT+TGG 0.575664 6_1:+85503809 None:intergenic
AGGTATAGAAATTGAAAAGG+TGG 0.588046 6_1:-85504635 Msa0896040:five_prime_UTR
TTCTACAACTTCTAATACCG+AGG 0.590215 6_1:+85504588 None:intergenic
GACCAAAGAAGATGAAGGAA+GGG 0.592744 6_1:-85503097 Msa0896040:three_prime_UTR
AATATGACCAAAGAAGATGA+AGG 0.618106 6_1:-85503102 Msa0896040:three_prime_UTR
AAATTAACATATCATCAATG+AGG 0.643337 6_1:+85504538 None:intergenic
TGTTGATAAGAAGATTACCT+CGG 0.656249 6_1:-85504605 Msa0896040:five_prime_UTR
TTTCTTTAATATGCACCCTG+AGG 0.665853 6_1:-85504507 Msa0896040:intron
ATGAAGTATATACTTCCCAG+AGG 0.760682 6_1:-85504655 Msa0896040:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATCTATTACTGTTATTTTT+TGG - chr6_1:85503427-85503446 Msa0896040:intron 15.0%
!!! ATCTATTACTGTTATTTTTT+GGG - chr6_1:85503428-85503447 Msa0896040:intron 15.0%
!!! TATCTATTACTGTTATTTTT+TGG - chr6_1:85503427-85503446 Msa0896040:intron 15.0%
!!! ATCTATTACTGTTATTTTTT+GGG - chr6_1:85503428-85503447 Msa0896040:intron 15.0%
!! AAATTAACATATCATCAATG+AGG + chr6_1:85503149-85503168 None:intergenic 20.0%
!!! ATTGTATTTTCTGTATTTGA+CGG - chr6_1:85503236-85503255 Msa0896040:intron 20.0%
!!! TAGTATTACATTGATTATGT+TGG + chr6_1:85503409-85503428 None:intergenic 20.0%
!!! TGAATTTATGTTTGCTATAA+TGG - chr6_1:85504346-85504365 Msa0896040:intron 20.0%
!!! TTAAGTTTTCCTTCTATTAT+TGG - chr6_1:85504757-85504776 Msa0896040:five_prime_UTR 20.0%
!! AAATTAACATATCATCAATG+AGG + chr6_1:85503149-85503168 None:intergenic 20.0%
!!! ATTGTATTTTCTGTATTTGA+CGG - chr6_1:85503236-85503255 Msa0896040:intron 20.0%
!!! TAGTATTACATTGATTATGT+TGG + chr6_1:85503409-85503428 None:intergenic 20.0%
!!! TGAATTTATGTTTGCTATAA+TGG - chr6_1:85504346-85504365 Msa0896040:intron 20.0%
!!! TTAAGTTTTCCTTCTATTAT+TGG - chr6_1:85504757-85504776 Msa0896040:five_prime_UTR 20.0%
!!! AGTGTTTGTTTTTGGAATAA+AGG - chr6_1:85502959-85502978 Msa0896040:three_prime_UTR 25.0%
!! TTTTCAATTTCTATACCTCT+GGG + chr6_1:85503047-85503066 None:intergenic 25.0%
! CTTAAAAAAATGTTACCTCA+GGG + chr6_1:85503195-85503214 None:intergenic 25.0%
!!! CTGAGGTAACATTTTTTTAA+GGG - chr6_1:85503194-85503213 Msa0896040:intron 25.0%
! AATTAATGCTATCTTAACAG+TGG - chr6_1:85503262-85503281 Msa0896040:intron 25.0%
!! TATTTTATATGCTAAGAGTC+CGG - chr6_1:85503327-85503346 Msa0896040:intron 25.0%
! GAATTAAAACCAAAACAAGA+TGG + chr6_1:85503680-85503699 None:intergenic 25.0%
!!! TCTTGTTTTGGTTTTAATTC+TGG - chr6_1:85503680-85503699 Msa0896040:intron 25.0%
! GTAATTGCTTTCTTGTTAAT+TGG - chr6_1:85503802-85503821 Msa0896040:CDS 25.0%
!!! TACTTTGAAATAAATGACAG+CGG + chr6_1:85504447-85504466 None:intergenic 25.0%
!!! AGTGTTTGTTTTTGGAATAA+AGG - chr6_1:85502959-85502978 Msa0896040:three_prime_UTR 25.0%
!! TTTTCAATTTCTATACCTCT+GGG + chr6_1:85503047-85503066 None:intergenic 25.0%
! CTTAAAAAAATGTTACCTCA+GGG + chr6_1:85503195-85503214 None:intergenic 25.0%
!!! CTGAGGTAACATTTTTTTAA+GGG - chr6_1:85503194-85503213 Msa0896040:intron 25.0%
! AATTAATGCTATCTTAACAG+TGG - chr6_1:85503262-85503281 Msa0896040:intron 25.0%
!! TATTTTATATGCTAAGAGTC+CGG - chr6_1:85503327-85503346 Msa0896040:intron 25.0%
! GAATTAAAACCAAAACAAGA+TGG + chr6_1:85503680-85503699 None:intergenic 25.0%
!!! TCTTGTTTTGGTTTTAATTC+TGG - chr6_1:85503680-85503699 Msa0896040:intron 25.0%
! GTAATTGCTTTCTTGTTAAT+TGG - chr6_1:85503802-85503821 Msa0896040:CDS 25.0%
!!! TACTTTGAAATAAATGACAG+CGG + chr6_1:85504447-85504466 None:intergenic 25.0%
! CTTTTCAATTTCTATACCTC+TGG + chr6_1:85503048-85503067 None:intergenic 30.0%
! CAGAGGTATAGAAATTGAAA+AGG - chr6_1:85503046-85503065 Msa0896040:three_prime_UTR 30.0%
AGGTATAGAAATTGAAAAGG+TGG - chr6_1:85503049-85503068 Msa0896040:three_prime_UTR 30.0%
! TGTTGATAAGAAGATTACCT+CGG - chr6_1:85503079-85503098 Msa0896040:three_prime_UTR 30.0%
CCTTAAAAAAATGTTACCTC+AGG + chr6_1:85503196-85503215 None:intergenic 30.0%
!!! CCTGAGGTAACATTTTTTTA+AGG - chr6_1:85503193-85503212 Msa0896040:intron 30.0%
ATTCAAGAATGCTACTGTAA+TGG - chr6_1:85503473-85503492 Msa0896040:intron 30.0%
TTGTGATTAGAGATTCCATA+TGG - chr6_1:85503531-85503550 Msa0896040:intron 30.0%
AGAAAGTAGTATACGAGATT+TGG - chr6_1:85503605-85503624 Msa0896040:intron 30.0%
GGATAACAATCTTTCATCAT+TGG - chr6_1:85503906-85503925 Msa0896040:intron 30.0%
GGTGTGATTAACTCTATATT+TGG - chr6_1:85503927-85503946 Msa0896040:intron 30.0%
! TGAAGAACAGATTGAGATTT+TGG - chr6_1:85504038-85504057 Msa0896040:intron 30.0%
!!! CCTTTGTTCTTTTAGAGTAT+CGG - chr6_1:85504144-85504163 Msa0896040:intron 30.0%
! TGAAGAACAGATTGAGATTT+TGG - chr6_1:85504038-85504057 Msa0896040:intron 30.0%
!!! CCTTTGTTCTTTTAGAGTAT+CGG - chr6_1:85504144-85504163 Msa0896040:intron 30.0%
!! AAATAAATGACAGCGGATTT+TGG + chr6_1:85504440-85504459 None:intergenic 30.0%
! TGTTAAGAATGCTTTGGATT+TGG - chr6_1:85504534-85504553 Msa0896040:five_prime_UTR 30.0%
AATATGACCAAAGAAGATGA+AGG - chr6_1:85504582-85504601 Msa0896040:five_prime_UTR 30.0%
!! AATTTTGAGTAACACCGATT+CGG - chr6_1:85504716-85504735 Msa0896040:five_prime_UTR 30.0%
TGAATGTATAAAGTCCGAAT+CGG + chr6_1:85504733-85504752 None:intergenic 30.0%
! TTTTCCTTCTATTATTGGAC+AGG - chr6_1:85504762-85504781 Msa0896040:five_prime_UTR 30.0%
AAATCCTGTCCAATAATAGA+AGG + chr6_1:85504769-85504788 None:intergenic 30.0%
! CTTTTCAATTTCTATACCTC+TGG + chr6_1:85503048-85503067 None:intergenic 30.0%
! CAGAGGTATAGAAATTGAAA+AGG - chr6_1:85503046-85503065 Msa0896040:three_prime_UTR 30.0%
AGGTATAGAAATTGAAAAGG+TGG - chr6_1:85503049-85503068 Msa0896040:three_prime_UTR 30.0%
! TGTTGATAAGAAGATTACCT+CGG - chr6_1:85503079-85503098 Msa0896040:three_prime_UTR 30.0%
CCTTAAAAAAATGTTACCTC+AGG + chr6_1:85503196-85503215 None:intergenic 30.0%
!!! CCTGAGGTAACATTTTTTTA+AGG - chr6_1:85503193-85503212 Msa0896040:intron 30.0%
ATTCAAGAATGCTACTGTAA+TGG - chr6_1:85503473-85503492 Msa0896040:intron 30.0%
TTGTGATTAGAGATTCCATA+TGG - chr6_1:85503531-85503550 Msa0896040:intron 30.0%
AGAAAGTAGTATACGAGATT+TGG - chr6_1:85503605-85503624 Msa0896040:intron 30.0%
GGATAACAATCTTTCATCAT+TGG - chr6_1:85503906-85503925 Msa0896040:intron 30.0%
GGTGTGATTAACTCTATATT+TGG - chr6_1:85503927-85503946 Msa0896040:intron 30.0%
! TGAAGAACAGATTGAGATTT+TGG - chr6_1:85504038-85504057 Msa0896040:intron 30.0%
!!! CCTTTGTTCTTTTAGAGTAT+CGG - chr6_1:85504144-85504163 Msa0896040:intron 30.0%
! TGAAGAACAGATTGAGATTT+TGG - chr6_1:85504038-85504057 Msa0896040:intron 30.0%
!!! CCTTTGTTCTTTTAGAGTAT+CGG - chr6_1:85504144-85504163 Msa0896040:intron 30.0%
!! AAATAAATGACAGCGGATTT+TGG + chr6_1:85504440-85504459 None:intergenic 30.0%
! TGTTAAGAATGCTTTGGATT+TGG - chr6_1:85504534-85504553 Msa0896040:five_prime_UTR 30.0%
AATATGACCAAAGAAGATGA+AGG - chr6_1:85504582-85504601 Msa0896040:five_prime_UTR 30.0%
!! AATTTTGAGTAACACCGATT+CGG - chr6_1:85504716-85504735 Msa0896040:five_prime_UTR 30.0%
TGAATGTATAAAGTCCGAAT+CGG + chr6_1:85504733-85504752 None:intergenic 30.0%
! TTTTCCTTCTATTATTGGAC+AGG - chr6_1:85504762-85504781 Msa0896040:five_prime_UTR 30.0%
AAATCCTGTCCAATAATAGA+AGG + chr6_1:85504769-85504788 None:intergenic 30.0%
! CCTCTATAGTCTAAGTGTTT+TGG + chr6_1:85502901-85502920 None:intergenic 35.0%
CCAAAACACTTAGACTATAG+AGG - chr6_1:85502898-85502917 Msa0896040:three_prime_UTR 35.0%
!! AGGACTGTAGTGTTTGTTTT+TGG - chr6_1:85502951-85502970 Msa0896040:three_prime_UTR 35.0%
ATGAAGTATATACTTCCCAG+AGG - chr6_1:85503029-85503048 Msa0896040:three_prime_UTR 35.0%
TTCTACAACTTCTAATACCG+AGG + chr6_1:85503099-85503118 None:intergenic 35.0%
TTTCTTTAATATGCACCCTG+AGG - chr6_1:85503177-85503196 Msa0896040:intron 35.0%
CTATCTTAACAGTGGTGTAT+GGG - chr6_1:85503270-85503289 Msa0896040:intron 35.0%
TCAGACTAGATTTCGAATTC+CGG + chr6_1:85503349-85503368 None:intergenic 35.0%
GAATTCGAAATCTAGTCTGA+GGG - chr6_1:85503349-85503368 Msa0896040:intron 35.0%
!! AGGGATTTCTGAAACTTCAT+TGG - chr6_1:85503368-85503387 Msa0896040:intron 35.0%
!!! ATTTTTTGGGAACATAGTCG+AGG - chr6_1:85503441-85503460 Msa0896040:intron 35.0%
!!! TTTTTTGGGAACATAGTCGA+GGG - chr6_1:85503442-85503461 Msa0896040:intron 35.0%
GCTTACATCTACTTACCATA+TGG + chr6_1:85503549-85503568 None:intergenic 35.0%
!! ATTTAGCTGCCATCTTGTTT+TGG - chr6_1:85503668-85503687 Msa0896040:intron 35.0%
CCTCAAAGGAATGACTAATT+AGG + chr6_1:85503852-85503871 None:intergenic 35.0%
CCTAATTAGTCATTCCTTTG+AGG - chr6_1:85503849-85503868 Msa0896040:CDS 35.0%
!! TCAAACTGGTTTGGATGAAT+CGG - chr6_1:85503885-85503904 Msa0896040:intron 35.0%
CTAATCTTATTCCTCCCAAT+AGG - chr6_1:85504009-85504028 Msa0896040:intron 35.0%
! CCGATACTCTAAAAGAACAA+AGG + chr6_1:85504147-85504166 None:intergenic 35.0%
! CCGATACTCTAAAAGAACAA+AGG + chr6_1:85504147-85504166 None:intergenic 35.0%
!!! TTAGAGTATCGGTCTCTTTT+AGG - chr6_1:85504155-85504174 Msa0896040:intron 35.0%
!!! TAGAGTATCGGTCTCTTTTA+GGG - chr6_1:85504156-85504175 Msa0896040:intron 35.0%
TTGCAGTGTTAAGAATGCTT+TGG - chr6_1:85504528-85504547 Msa0896040:five_prime_UTR 35.0%
TCTGTACTGCAAATGATTGT+TGG - chr6_1:85504638-85504657 Msa0896040:five_prime_UTR 35.0%
!! CAAATGATTGTTGGTAGCAA+GGG - chr6_1:85504647-85504666 Msa0896040:five_prime_UTR 35.0%
! CCTCTATAGTCTAAGTGTTT+TGG + chr6_1:85502901-85502920 None:intergenic 35.0%
CCAAAACACTTAGACTATAG+AGG - chr6_1:85502898-85502917 Msa0896040:three_prime_UTR 35.0%
!! AGGACTGTAGTGTTTGTTTT+TGG - chr6_1:85502951-85502970 Msa0896040:three_prime_UTR 35.0%
ATGAAGTATATACTTCCCAG+AGG - chr6_1:85503029-85503048 Msa0896040:three_prime_UTR 35.0%
TTCTACAACTTCTAATACCG+AGG + chr6_1:85503099-85503118 None:intergenic 35.0%
TTTCTTTAATATGCACCCTG+AGG - chr6_1:85503177-85503196 Msa0896040:intron 35.0%
CTATCTTAACAGTGGTGTAT+GGG - chr6_1:85503270-85503289 Msa0896040:intron 35.0%
TCAGACTAGATTTCGAATTC+CGG + chr6_1:85503349-85503368 None:intergenic 35.0%
GAATTCGAAATCTAGTCTGA+GGG - chr6_1:85503349-85503368 Msa0896040:intron 35.0%
!! AGGGATTTCTGAAACTTCAT+TGG - chr6_1:85503368-85503387 Msa0896040:intron 35.0%
!!! ATTTTTTGGGAACATAGTCG+AGG - chr6_1:85503441-85503460 Msa0896040:intron 35.0%
!!! TTTTTTGGGAACATAGTCGA+GGG - chr6_1:85503442-85503461 Msa0896040:intron 35.0%
GCTTACATCTACTTACCATA+TGG + chr6_1:85503549-85503568 None:intergenic 35.0%
!! ATTTAGCTGCCATCTTGTTT+TGG - chr6_1:85503668-85503687 Msa0896040:intron 35.0%
CCTCAAAGGAATGACTAATT+AGG + chr6_1:85503852-85503871 None:intergenic 35.0%
CCTAATTAGTCATTCCTTTG+AGG - chr6_1:85503849-85503868 Msa0896040:CDS 35.0%
!! TCAAACTGGTTTGGATGAAT+CGG - chr6_1:85503885-85503904 Msa0896040:intron 35.0%
CTAATCTTATTCCTCCCAAT+AGG - chr6_1:85504009-85504028 Msa0896040:intron 35.0%
! CCGATACTCTAAAAGAACAA+AGG + chr6_1:85504147-85504166 None:intergenic 35.0%
! CCGATACTCTAAAAGAACAA+AGG + chr6_1:85504147-85504166 None:intergenic 35.0%
!!! TTAGAGTATCGGTCTCTTTT+AGG - chr6_1:85504155-85504174 Msa0896040:intron 35.0%
!!! TAGAGTATCGGTCTCTTTTA+GGG - chr6_1:85504156-85504175 Msa0896040:intron 35.0%
TTGCAGTGTTAAGAATGCTT+TGG - chr6_1:85504528-85504547 Msa0896040:five_prime_UTR 35.0%
TCTGTACTGCAAATGATTGT+TGG - chr6_1:85504638-85504657 Msa0896040:five_prime_UTR 35.0%
!! CAAATGATTGTTGGTAGCAA+GGG - chr6_1:85504647-85504666 Msa0896040:five_prime_UTR 35.0%
GCTATCTTAACAGTGGTGTA+TGG - chr6_1:85503269-85503288 Msa0896040:intron 40.0%
GTGGTGTATGGGATCTTATT+CGG - chr6_1:85503281-85503300 Msa0896040:intron 40.0%
GGAATTCGAAATCTAGTCTG+AGG - chr6_1:85503348-85503367 Msa0896040:intron 40.0%
!! ATCTACGTTGGTTTCCTCAA+AGG + chr6_1:85503866-85503885 None:intergenic 40.0%
GAAACCAACGTAGATCAAAC+TGG - chr6_1:85503871-85503890 Msa0896040:intron 40.0%
CAAACCAGTTTGATCTACGT+TGG + chr6_1:85503878-85503897 None:intergenic 40.0%
CAACGTAGATCAAACTGGTT+TGG - chr6_1:85503876-85503895 Msa0896040:intron 40.0%
GTTCTTCAGCTTCTCCTATT+GGG + chr6_1:85504026-85504045 None:intergenic 40.0%
TGTTCTTCAGCTTCTCCTAT+TGG + chr6_1:85504027-85504046 None:intergenic 40.0%
CTTGTAAAGGTGGTTTCTTC+TGG - chr6_1:85504081-85504100 Msa0896040:intron 40.0%
CTTGTAAAGGTGGTTTCTTC+TGG - chr6_1:85504081-85504100 Msa0896040:intron 40.0%
TGACCAAAGAAGATGAAGGA+AGG - chr6_1:85504586-85504605 Msa0896040:five_prime_UTR 40.0%
GACCAAAGAAGATGAAGGAA+GGG - chr6_1:85504587-85504606 Msa0896040:five_prime_UTR 40.0%
AACCCTTCCTTCATCTTCTT+TGG + chr6_1:85504592-85504611 None:intergenic 40.0%
!! GCAAATGATTGTTGGTAGCA+AGG - chr6_1:85504646-85504665 Msa0896040:five_prime_UTR 40.0%
GCTATCTTAACAGTGGTGTA+TGG - chr6_1:85503269-85503288 Msa0896040:intron 40.0%
GTGGTGTATGGGATCTTATT+CGG - chr6_1:85503281-85503300 Msa0896040:intron 40.0%
GGAATTCGAAATCTAGTCTG+AGG - chr6_1:85503348-85503367 Msa0896040:intron 40.0%
!! ATCTACGTTGGTTTCCTCAA+AGG + chr6_1:85503866-85503885 None:intergenic 40.0%
GAAACCAACGTAGATCAAAC+TGG - chr6_1:85503871-85503890 Msa0896040:intron 40.0%
CAAACCAGTTTGATCTACGT+TGG + chr6_1:85503878-85503897 None:intergenic 40.0%
CAACGTAGATCAAACTGGTT+TGG - chr6_1:85503876-85503895 Msa0896040:intron 40.0%
GTTCTTCAGCTTCTCCTATT+GGG + chr6_1:85504026-85504045 None:intergenic 40.0%
TGTTCTTCAGCTTCTCCTAT+TGG + chr6_1:85504027-85504046 None:intergenic 40.0%
CTTGTAAAGGTGGTTTCTTC+TGG - chr6_1:85504081-85504100 Msa0896040:intron 40.0%
CTTGTAAAGGTGGTTTCTTC+TGG - chr6_1:85504081-85504100 Msa0896040:intron 40.0%
TGACCAAAGAAGATGAAGGA+AGG - chr6_1:85504586-85504605 Msa0896040:five_prime_UTR 40.0%
GACCAAAGAAGATGAAGGAA+GGG - chr6_1:85504587-85504606 Msa0896040:five_prime_UTR 40.0%
AACCCTTCCTTCATCTTCTT+TGG + chr6_1:85504592-85504611 None:intergenic 40.0%
!! GCAAATGATTGTTGGTAGCA+AGG - chr6_1:85504646-85504665 Msa0896040:five_prime_UTR 40.0%
CATTTGCGCTAGATGAGCAT+AGG + chr6_1:85503012-85503031 None:intergenic 45.0%
GGAACATAGTCGAGGGATTA+TGG - chr6_1:85503449-85503468 Msa0896040:intron 45.0%
!! TGTGCCAGACTAGCTTGTAA+AGG - chr6_1:85504068-85504087 Msa0896040:intron 45.0%
ACCACCTTTACAAGCTAGTC+TGG + chr6_1:85504075-85504094 None:intergenic 45.0%
! CTTCTGGAGACACTGTGTAA+TGG - chr6_1:85504097-85504116 Msa0896040:intron 45.0%
TGGAGACACTGTGTAATGGT+GGG - chr6_1:85504101-85504120 Msa0896040:intron 45.0%
!! TGTGCCAGACTAGCTTGTAA+AGG - chr6_1:85504068-85504087 Msa0896040:intron 45.0%
ACCACCTTTACAAGCTAGTC+TGG + chr6_1:85504075-85504094 None:intergenic 45.0%
! CTTCTGGAGACACTGTGTAA+TGG - chr6_1:85504097-85504116 Msa0896040:intron 45.0%
TGGAGACACTGTGTAATGGT+GGG - chr6_1:85504101-85504120 Msa0896040:intron 45.0%
CATTTGCGCTAGATGAGCAT+AGG + chr6_1:85503012-85503031 None:intergenic 45.0%
GGAACATAGTCGAGGGATTA+TGG - chr6_1:85503449-85503468 Msa0896040:intron 45.0%
!! TGTGCCAGACTAGCTTGTAA+AGG - chr6_1:85504068-85504087 Msa0896040:intron 45.0%
ACCACCTTTACAAGCTAGTC+TGG + chr6_1:85504075-85504094 None:intergenic 45.0%
! CTTCTGGAGACACTGTGTAA+TGG - chr6_1:85504097-85504116 Msa0896040:intron 45.0%
TGGAGACACTGTGTAATGGT+GGG - chr6_1:85504101-85504120 Msa0896040:intron 45.0%
!! TGTGCCAGACTAGCTTGTAA+AGG - chr6_1:85504068-85504087 Msa0896040:intron 45.0%
ACCACCTTTACAAGCTAGTC+TGG + chr6_1:85504075-85504094 None:intergenic 45.0%
! CTTCTGGAGACACTGTGTAA+TGG - chr6_1:85504097-85504116 Msa0896040:intron 45.0%
TGGAGACACTGTGTAATGGT+GGG - chr6_1:85504101-85504120 Msa0896040:intron 45.0%
!! AATTTATTATTAAAAAGAAA+TGG + chr6_1:85502926-85502945 None:intergenic 5.0%
!!! TTTTAATAATAAATTAAGAA+AGG - chr6_1:85502931-85502950 Msa0896040:three_prime_UTR 5.0%
!! AATTTATTATTAAAAAGAAA+TGG + chr6_1:85502926-85502945 None:intergenic 5.0%
!!! TTTTAATAATAAATTAAGAA+AGG - chr6_1:85502931-85502950 Msa0896040:three_prime_UTR 5.0%
CTTCAGCTTCTCCTATTGGG+AGG + chr6_1:85504023-85504042 None:intergenic 50.0%
!! GCCAGACTAGCTTGTAAAGG+TGG - chr6_1:85504071-85504090 Msa0896040:intron 50.0%
CTGGAGACACTGTGTAATGG+TGG - chr6_1:85504100-85504119 Msa0896040:intron 50.0%
!! GCCAGACTAGCTTGTAAAGG+TGG - chr6_1:85504071-85504090 Msa0896040:intron 50.0%
CTGGAGACACTGTGTAATGG+TGG - chr6_1:85504100-85504119 Msa0896040:intron 50.0%
CTTCAGCTTCTCCTATTGGG+AGG + chr6_1:85504023-85504042 None:intergenic 50.0%
!! GCCAGACTAGCTTGTAAAGG+TGG - chr6_1:85504071-85504090 Msa0896040:intron 50.0%
CTGGAGACACTGTGTAATGG+TGG - chr6_1:85504100-85504119 Msa0896040:intron 50.0%
!! GCCAGACTAGCTTGTAAAGG+TGG - chr6_1:85504071-85504090 Msa0896040:intron 50.0%
CTGGAGACACTGTGTAATGG+TGG - chr6_1:85504100-85504119 Msa0896040:intron 50.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 85502892 85504814 85502892 ID=Msa0896040;Name=Msa0896040
chr6_1 mRNA 85502892 85504814 85502892 ID=Msa0896040-mRNA-1;Parent=Msa0896040;Name=Msa0896040-mRNA-1;_AED=0.16;_eAED=0.22;_QI=295|0.33|0.5|1|0|0|4|244|62
chr6_1 exon 85502892 85503172 85502892 ID=Msa0896040-mRNA-1:exon:14414;Parent=Msa0896040-mRNA-1
chr6_1 exon 85503480 85503529 85503480 ID=Msa0896040-mRNA-1:exon:14413;Parent=Msa0896040-mRNA-1
chr6_1 exon 85503779 85503868 85503779 ID=Msa0896040-mRNA-1:exon:14412;Parent=Msa0896040-mRNA-1
chr6_1 exon 85504508 85504814 85504508 ID=Msa0896040-mRNA-1:exon:14411;Parent=Msa0896040-mRNA-1
chr6_1 five_prime_UTR 85504520 85504814 85504520 ID=Msa0896040-mRNA-1:five_prime_utr;Parent=Msa0896040-mRNA-1
chr6_1 CDS 85504508 85504519 85504508 ID=Msa0896040-mRNA-1:cds;Parent=Msa0896040-mRNA-1
chr6_1 CDS 85503779 85503868 85503779 ID=Msa0896040-mRNA-1:cds;Parent=Msa0896040-mRNA-1
chr6_1 CDS 85503480 85503529 85503480 ID=Msa0896040-mRNA-1:cds;Parent=Msa0896040-mRNA-1
chr6_1 CDS 85503136 85503172 85503136 ID=Msa0896040-mRNA-1:cds;Parent=Msa0896040-mRNA-1
chr6_1 three_prime_UTR 85502892 85503135 85502892 ID=Msa0896040-mRNA-1:three_prime_utr;Parent=Msa0896040-mRNA-1
Gene Sequence

>Msa0896040

ATGCACCCTGAGTTAGAAGAAAGCCTAATTAGTCATTCCTTTGAGGAAACCAACGTAGATCAAACTGGTTTGGATGAATCGGATAACAATCTTTCATCATTGAAGCTGAAGAACAGATTGAGATTTTGGAAAAGATTGTGCCAGACTAGCTTTGTTAAGAATGCTTTGGATTTGGACTCAGTCGACTAA
Protein sequence

>Msa0896040

MHPELEESLISHSFEETNVDQTGLDESDNNLSSLKLKNRLRFWKRLCQTSFVKNALDLDSVD*