AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0596840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0596840 MtrunA17_Chr4g0019341 66.000 100 19 3 1 90 1 95 3.91e-35 115
Msa0596840 MtrunA17_Chr4g0019351 87.879 66 8 0 1 66 1 66 6.19e-35 114
Msa0596840 MtrunA17_Chr4g0019361 81.481 54 10 0 1 54 1 54 7.43e-23 83.6
Msa0596840 MtrunA17_Chr4g0018981 53.846 78 22 2 1 65 1 77 7.82e-19 73.6
Msa0596840 MtrunA17_Chr4g0018971 53.333 75 25 1 1 65 1 75 5.40e-18 71.2
Msa0596840 MtrunA17_Chr4g0018961 48.000 75 32 2 1 75 1 68 1.01e-14 63.2
Msa0596840 MtrunA17_Chr8g0368881 40.964 83 45 2 1 82 1 80 1.22e-13 60.5
Msa0596840 MtrunA17_Chr4g0018931 45.070 71 37 2 1 70 1 70 2.64e-11 54.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 486 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCAACAACCACCGATTT+TGG 0.161774 4_2:-28432755 Msa0596840:five_prime_UTR
AAAGAAGTGCTCTCTTATTT+TGG 0.217478 4_2:-28432679 Msa0596840:five_prime_UTR
CATTCAATTAATAATGTATC+TGG 0.253566 4_2:-28425624 Msa0596840:three_prime_UTR
CTTATAATTAATATAGTATA+GGG 0.264038 4_2:+28425667 None:intergenic
TGTATAGTTTCAGATGAAAT+AGG 0.265626 4_2:-28426090 Msa0596840:intron
TGTATAGTTTCAGATGAAAT+AGG 0.330215 4_2:-28432465 Msa0596840:intron
AGAAGAAATAAATCTGAATA+AGG 0.350050 4_2:-28425892 Msa0596840:CDS
AGAAGAAATAAATCTGAATA+AGG 0.350050 4_2:-28432262 Msa0596840:five_prime_UTR
TGTTAGGTTGGATCAATTTA+CGG 0.354656 4_2:-28425807 Msa0596840:three_prime_UTR
AAAGAAGTGCTCTCTTGTTT+TGG 0.355506 4_2:-28426302 Msa0596840:CDS
ACTTATAATTAATATAGTAT+AGG 0.356165 4_2:+28425666 None:intergenic
ACAATTATCCATGAATGTTA+TGG 0.358827 4_2:-28425833 Msa0596840:exon
TTGTGGCCACGTGCATTATC+AGG 0.363902 4_2:+28426027 None:intergenic
TTGTGGCCACGTGCATTATC+AGG 0.363902 4_2:+28432402 None:intergenic
CAATTATCCATGAATGTTAT+GGG 0.399405 4_2:-28425832 Msa0596840:exon
ATGAATGTTATGGGCGTGTT+AGG 0.399649 4_2:-28425823 Msa0596840:exon
TAACACGCCCATAACATTCA+TGG 0.407703 4_2:+28425825 None:intergenic
TAATTTATATGTTCCAAAAT+CGG 0.422260 4_2:+28432742 None:intergenic
TTATATATAAGCACATAGAT+TGG 0.428764 4_2:+28425469 None:intergenic
AATTCAAACAATTATGTATG+TGG 0.434652 4_2:+28425588 None:intergenic
ACACTCGAGCTTTCCACTTA+GGG 0.436414 4_2:+28425712 None:intergenic
ATATGATTTATTTACTTGAA+AGG 0.450066 4_2:-28432778 None:intergenic
TACAATGGACTCAATACATA+TGG 0.465677 4_2:+28432719 None:intergenic
AACACTCGAGCTTTCCACTT+AGG 0.491057 4_2:+28425711 None:intergenic
CCACGTGCATTATCAGGCGC+AGG 0.523034 4_2:+28426033 None:intergenic
CCACGTGCATTATCAGGCGC+AGG 0.523034 4_2:+28432408 None:intergenic
AGTTTGTTTATAATTAGAAA+CGG 0.526482 4_2:-28425551 Msa0596840:three_prime_UTR
GCACGTGGCCACAATTATGC+AGG 0.531418 4_2:-28426018 Msa0596840:intron
GATCAATTTACGGATGAAGT+TGG 0.531820 4_2:-28425797 Msa0596840:three_prime_UTR
ATGTTATGGGCGTGTTAGGT+TGG 0.561326 4_2:-28425819 Msa0596840:three_prime_UTR
TTTATATGTTCCAAAATCGG+TGG 0.576643 4_2:+28432745 None:intergenic
TTTCATCAAAGGCATTAGCA+AGG 0.590558 4_2:-28426252 Msa0596840:CDS
TTTCATCAAAGGCATTAGCA+AGG 0.590558 4_2:-28432629 Msa0596840:five_prime_UTR
TTGTTTATAATTAGAAACGG+AGG 0.590744 4_2:-28425548 Msa0596840:three_prime_UTR
ATAAGGAGTCTGATACAGTA+AGG 0.599361 4_2:-28425875 Msa0596840:CDS
ATAAGGAGTCTGATACAGTA+AGG 0.599361 4_2:-28432245 Msa0596840:five_prime_UTR
TGTTTATAATTAGAAACGGA+GGG 0.600307 4_2:-28425547 Msa0596840:three_prime_UTR
GCGCAGGCTTCTCACTAGCA+AGG 0.617322 4_2:+28426049 None:intergenic
GCGCAGGCTTCTCACTAGCA+AGG 0.617322 4_2:+28432424 None:intergenic
ATAACATTCATGGATAATTG+TGG 0.619531 4_2:+28425835 None:intergenic
CCTGCGCCTGATAATGCACG+TGG 0.653177 4_2:-28426033 Msa0596840:CDS
CCTGCGCCTGATAATGCACG+TGG 0.653177 4_2:-28432408 Msa0596840:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTATTATGGATATTAT+GGG + chr4_2:28425870-28425889 None:intergenic 10.0%
!! AAGAAAAAACTTAATTATTA+TGG + chr4_2:28425880-28425899 None:intergenic 10.0%
!!! TTTTTTCTTGTATTAATATT+TGG - chr4_2:28425891-28425910 Msa0596840:CDS 10.0%
!! CTTATAATTAATATAGTATA+GGG + chr4_2:28426159-28426178 None:intergenic 10.0%
!! ACTTATAATTAATATAGTAT+AGG + chr4_2:28426160-28426179 None:intergenic 10.0%
!!! ATAAGTAAATTTGAATTTTT+AGG - chr4_2:28426174-28426193 Msa0596840:intron 10.0%
!! AAAGAAATTATTATCATAAA+AGG - chr4_2:28426507-28426526 Msa0596840:intron 10.0%
!!! ATGGTTTATAAATTTTAAAA+AGG - chr4_2:28427098-28427117 Msa0596840:intron 10.0%
!!! TGGTTTATAAATTTTAAAAA+GGG - chr4_2:28427099-28427118 Msa0596840:intron 10.0%
!!! CTCTTTTTAATAATTTTTTT+AGG + chr4_2:28428389-28428408 None:intergenic 10.0%
!!! TATCGAAATAAATTTTATTT+AGG + chr4_2:28431412-28431431 None:intergenic 10.0%
!! GTAAATAAATCATATTTAAT+TGG + chr4_2:28431787-28431806 None:intergenic 10.0%
!! TTAATTATTATGGATATTAT+GGG + chr4_2:28425870-28425889 None:intergenic 10.0%
!! AAGAAAAAACTTAATTATTA+TGG + chr4_2:28425880-28425899 None:intergenic 10.0%
!!! TTTTTTCTTGTATTAATATT+TGG - chr4_2:28425891-28425910 Msa0596840:CDS 10.0%
!! CTTATAATTAATATAGTATA+GGG + chr4_2:28426159-28426178 None:intergenic 10.0%
!! ACTTATAATTAATATAGTAT+AGG + chr4_2:28426160-28426179 None:intergenic 10.0%
!!! ATAAGTAAATTTGAATTTTT+AGG - chr4_2:28426174-28426193 Msa0596840:intron 10.0%
!! TAATTTATATGTTCCAAAAT+CGG + chr4_2:28425454-28425473 None:intergenic 15.0%
!! AATAACAAAATGGATATTAT+GGG + chr4_2:28425634-28425653 None:intergenic 15.0%
!!! GTTTTTCTTGTATTAATATA+TGG - chr4_2:28425699-28425718 Msa0596840:three_prime_UTR 15.0%
!!! GCAAGTTTTTTTTTTTTTTT+TGG - chr4_2:28425818-28425837 Msa0596840:three_prime_UTR 15.0%
!! TAATTATTATGGATATTATG+GGG + chr4_2:28425869-28425888 None:intergenic 15.0%
!! CTTAATTATTATGGATATTA+TGG + chr4_2:28425871-28425890 None:intergenic 15.0%
!! TTGGTAGAAAAAATAAATAA+TGG - chr4_2:28426045-28426064 Msa0596840:CDS 15.0%
!! TGGTAGAAAAAATAAATAAT+GGG - chr4_2:28426046-28426065 Msa0596840:CDS 15.0%
!! AATTCAATTAATTATGTATG+TGG + chr4_2:28426238-28426257 None:intergenic 15.0%
!!! AATATTTTAGTTAATACACA+AGG - chr4_2:28426438-28426457 Msa0596840:intron 15.0%
!!! ATATTTTAGTTAATACACAA+GGG - chr4_2:28426439-28426458 Msa0596840:intron 15.0%
!!! TTTAATAATTTTTTTAGGGT+GGG + chr4_2:28428384-28428403 None:intergenic 15.0%
!!! TTTTAATAATTTTTTTAGGG+TGG + chr4_2:28428385-28428404 None:intergenic 15.0%
!!! AACTTGTTATTAAAAGATAT+CGG + chr4_2:28429379-28429398 None:intergenic 15.0%
!!! TTATTTTTAGTATTAACAGT+AGG - chr4_2:28430005-28430024 Msa0596840:intron 15.0%
!!! TATTTTTAGTATTAACAGTA+GGG - chr4_2:28430006-28430025 Msa0596840:intron 15.0%
!!! ACCAAAATTTTTTATGTTTT+TGG + chr4_2:28430125-28430144 None:intergenic 15.0%
!!! TTTATCATTTTTAGAGTTTA+TGG - chr4_2:28430761-28430780 Msa0596840:intron 15.0%
!! TAGAGTATAAAACTATTTAT+TGG - chr4_2:28430896-28430915 Msa0596840:intron 15.0%
!! CTATATAAAATAACAATGTT+TGG + chr4_2:28430970-28430989 None:intergenic 15.0%
!! ACTTTAAATCAAAAATAGTT+TGG + chr4_2:28431531-28431550 None:intergenic 15.0%
!! GTTAATAGAAATATTTACTA+AGG + chr4_2:28431558-28431577 None:intergenic 15.0%
!!! TGATTGATTTACTTTTTTTT+AGG + chr4_2:28431662-28431681 None:intergenic 15.0%
!! ATATGATTTATTTACTTGAA+AGG - chr4_2:28431792-28431811 Msa0596840:intron 15.0%
!! TAATTTATATGTTCCAAAAT+CGG + chr4_2:28425454-28425473 None:intergenic 15.0%
!! AATAACAAAATGGATATTAT+GGG + chr4_2:28425634-28425653 None:intergenic 15.0%
!!! GTTTTTCTTGTATTAATATA+TGG - chr4_2:28425699-28425718 Msa0596840:three_prime_UTR 15.0%
!! TAATTATTATGGATATTATG+GGG + chr4_2:28425869-28425888 None:intergenic 15.0%
!! CTTAATTATTATGGATATTA+TGG + chr4_2:28425871-28425890 None:intergenic 15.0%
!! TTGGTAGAAAAAATAAATAA+TGG - chr4_2:28426045-28426064 Msa0596840:CDS 15.0%
!! TGGTAGAAAAAATAAATAAT+GGG - chr4_2:28426046-28426065 Msa0596840:CDS 15.0%
!!! AGTTTGTTTATAATTAGAAA+CGG - chr4_2:28432642-28432661 Msa0596840:five_prime_UTR 15.0%
!! ATAACAAAATGGATATTATG+GGG + chr4_2:28425633-28425652 None:intergenic 20.0%
!! CAATAACAAAATGGATATTA+TGG + chr4_2:28425635-28425654 None:intergenic 20.0%
!! AAAAAAAACTTGCATAATTG+TGG + chr4_2:28425811-28425830 None:intergenic 20.0%
!! AGAAGAAATAAATCTGAATA+AGG - chr4_2:28425931-28425950 Msa0596840:intron 20.0%
!!! AGTTTGCTTATAATTAGAAA+CGG - chr4_2:28426272-28426291 Msa0596840:CDS 20.0%
!! TTATATATAAGCACATAGAT+TGG + chr4_2:28426357-28426376 None:intergenic 20.0%
!! ACTGATTAAAGATACTAAAA+AGG + chr4_2:28426598-28426617 None:intergenic 20.0%
!! GTTTATTAGAAAATGTTAAC+CGG + chr4_2:28426632-28426651 None:intergenic 20.0%
!! GGACAAAAATAAAATCAAAT+GGG + chr4_2:28426695-28426714 None:intergenic 20.0%
!! ATGTATCTAGTAATTTGAAA+AGG + chr4_2:28426731-28426750 None:intergenic 20.0%
!! AAGATAGTAGTATTAATTAG+GGG + chr4_2:28426777-28426796 None:intergenic 20.0%
!! CAAGATAGTAGTATTAATTA+GGG + chr4_2:28426778-28426797 None:intergenic 20.0%
!! ACAAGATAGTAGTATTAATT+AGG + chr4_2:28426779-28426798 None:intergenic 20.0%
!! TTTAATACACATACAAAGAA+AGG - chr4_2:28426820-28426839 Msa0596840:intron 20.0%
!! AAAATTGACAGCAAATTATA+TGG - chr4_2:28427043-28427062 Msa0596840:intron 20.0%
!!! TTGTATCTTCTTATTGTTTT+AGG + chr4_2:28428072-28428091 None:intergenic 20.0%
!! ATTTGTAAGAATAAGGAATA+TGG + chr4_2:28428319-28428338 None:intergenic 20.0%
!! AGAGTATATTTGTAAGAATA+AGG + chr4_2:28428326-28428345 None:intergenic 20.0%
!! ATTAAAAAGAGATTCAATAG+AGG - chr4_2:28428398-28428417 Msa0596840:intron 20.0%
!! TTAAATCTAGATTATCTGAA+TGG + chr4_2:28428533-28428552 None:intergenic 20.0%
!! TAATGATTCTTAGTTAAATG+AGG + chr4_2:28428664-28428683 None:intergenic 20.0%
!! CAAGAAAAATATTAAATTGC+AGG - chr4_2:28429498-28429517 Msa0596840:intron 20.0%
!!! TATTTTTTTTTGTAGACCTA+TGG + chr4_2:28430075-28430094 None:intergenic 20.0%
!!! GCCAAAAACATAAAAAATTT+TGG - chr4_2:28430121-28430140 Msa0596840:intron 20.0%
!! TTCATTGAATTAAACAAGAT+GGG + chr4_2:28430203-28430222 None:intergenic 20.0%
!! TTTCATTGAATTAAACAAGA+TGG + chr4_2:28430204-28430223 None:intergenic 20.0%
!!! ATCTTGTTTAATTCAATGAA+AGG - chr4_2:28430203-28430222 Msa0596840:intron 20.0%
!! GCAGTTCATTAATATTTAAT+AGG - chr4_2:28430233-28430252 Msa0596840:intron 20.0%
!! AATATTTAATAGGAATCCTT+AGG - chr4_2:28430243-28430262 Msa0596840:intron 20.0%
!!! ATATGTTTTTGTTCTGAATT+GGG + chr4_2:28430863-28430882 None:intergenic 20.0%
!!! ATTTATTGGTTAACTTTCAA+AGG - chr4_2:28430910-28430929 Msa0596840:intron 20.0%
!!! AAAAAAGTGTGATTTTTTGT+AGG - chr4_2:28430935-28430954 Msa0596840:intron 20.0%
!!! AAAAAGTGTGATTTTTTGTA+GGG - chr4_2:28430936-28430955 Msa0596840:intron 20.0%
!!! CTTTTCTTTTTTAGTTCAAA+CGG - chr4_2:28431577-28431596 Msa0596840:intron 20.0%
!!! TTTTAGGAATGATTATATAG+AGG + chr4_2:28431646-28431665 None:intergenic 20.0%
!!! AAGTTTTTACTTTTTTTACG+CGG + chr4_2:28431753-28431772 None:intergenic 20.0%
!! ATAACAAAATGGATATTATG+GGG + chr4_2:28425633-28425652 None:intergenic 20.0%
!! CAATAACAAAATGGATATTA+TGG + chr4_2:28425635-28425654 None:intergenic 20.0%
!!! ATTATGCAGGTTTTTTTTTT+TGG - chr4_2:28432188-28432207 Msa0596840:intron 20.0%
!! AGAAGAAATAAATCTGAATA+AGG - chr4_2:28425931-28425950 Msa0596840:intron 20.0%
!! CATTCAATTAATAATGTATC+TGG - chr4_2:28432569-28432588 Msa0596840:intron 20.0%
!! AATTCAAACAATTATGTATG+TGG + chr4_2:28432608-28432627 None:intergenic 20.0%
!! TTATATATAAGCACATAGAT+TGG + chr4_2:28426357-28426376 None:intergenic 20.0%
! TGTATAGTTTCAGATGAAAT+AGG - chr4_2:28425728-28425747 Msa0596840:three_prime_UTR 25.0%
! ATAACATTCATGGATAATTG+TGG + chr4_2:28425991-28426010 None:intergenic 25.0%
! ACAATTATCCATGAATGTTA+TGG - chr4_2:28425990-28426009 Msa0596840:intron 25.0%
! CAATTATCCATGAATGTTAT+GGG - chr4_2:28425991-28426010 Msa0596840:intron 25.0%
!!! TTTGATTTATTTTCCCTAAG+TGG - chr4_2:28426098-28426117 Msa0596840:intron 25.0%
! CATTCAATTAATGATGTATG+TGG - chr4_2:28426199-28426218 Msa0596840:intron 25.0%
! GGGACAAAAATAAAATCAAA+TGG + chr4_2:28426696-28426715 None:intergenic 25.0%
!! TATTAATTAGGGGTATGTTT+AGG + chr4_2:28426767-28426786 None:intergenic 25.0%
! AATTAACACAATTCACATGA+TGG - chr4_2:28427079-28427098 Msa0596840:intron 25.0%
! CATAGATACTTGAAGAATTA+GGG + chr4_2:28427173-28427192 None:intergenic 25.0%
! ACATAGATACTTGAAGAATT+AGG + chr4_2:28427174-28427193 None:intergenic 25.0%
! TTCATATAATTTCCTTCATC+TGG + chr4_2:28427706-28427725 None:intergenic 25.0%
!! TTTGATGATATGACTTATGA+AGG - chr4_2:28427829-28427848 Msa0596840:intron 25.0%
!! ATATTGCTTTGTTAGGAAAA+TGG + chr4_2:28428264-28428283 None:intergenic 25.0%
!! GATTTCAATATTGCTTTGTT+AGG + chr4_2:28428271-28428290 None:intergenic 25.0%
! GATTTAATTCTAGCCAAAAT+TGG - chr4_2:28428546-28428565 Msa0596840:intron 25.0%
!! AAGATAAGTTTTGCACAAAT+TGG + chr4_2:28429108-28429127 None:intergenic 25.0%
! ACTCTCAAAACATTACAAAA+AGG - chr4_2:28429170-28429189 Msa0596840:intron 25.0%
! TAAACTTCATTATTCGTCAA+TGG - chr4_2:28429237-28429256 Msa0596840:intron 25.0%
!! CACTTTAAAAAGACATTATC+CGG + chr4_2:28429270-28429289 None:intergenic 25.0%
! AAAGATGAAAAGAAATGCAA+AGG - chr4_2:28429464-28429483 Msa0596840:intron 25.0%
! AAGATGAAAAGAAATGCAAA+GGG - chr4_2:28429465-28429484 Msa0596840:intron 25.0%
! TTAAATTGCAGGAATGTAAA+TGG - chr4_2:28429509-28429528 Msa0596840:intron 25.0%
!!! CAACTTTTAATTTAGACTGT+AGG + chr4_2:28429711-28429730 None:intergenic 25.0%
!! AAATAATCTGAAGTTACAGT+TGG + chr4_2:28429820-28429839 None:intergenic 25.0%
!!! AGATTATTTCTACTTGTCTT+TGG - chr4_2:28429831-28429850 Msa0596840:intron 25.0%
! TGTTTAATTCAATGAAAGGA+AGG - chr4_2:28430207-28430226 Msa0596840:intron 25.0%
! TAAACTATATACACACTTTG+AGG + chr4_2:28430356-28430375 None:intergenic 25.0%
! ATTGTCTCAAATTTACAGTA+AGG - chr4_2:28430396-28430415 Msa0596840:intron 25.0%
! GTATGTTGAGATGATTTATT+TGG - chr4_2:28430619-28430638 Msa0596840:intron 25.0%
! GAAAAATATTTATGCATGCT+TGG - chr4_2:28430682-28430701 Msa0596840:intron 25.0%
!!! CATATGTTTTTGTTCTGAAT+TGG + chr4_2:28430864-28430883 None:intergenic 25.0%
!! ACTTGTTAATCACAATAGAA+TGG - chr4_2:28431041-28431060 Msa0596840:intron 25.0%
! CTTGTTAATCACAATAGAAT+GGG - chr4_2:28431042-28431061 Msa0596840:intron 25.0%
! TTGTTAATCACAATAGAATG+GGG - chr4_2:28431043-28431062 Msa0596840:intron 25.0%
! TACACATCATTTATCAAAGA+AGG + chr4_2:28431147-28431166 None:intergenic 25.0%
!! TTTTAGATGACAACAAAATG+TGG - chr4_2:28431246-28431265 Msa0596840:intron 25.0%
! TGTATAGTTTCAGATGAAAT+AGG - chr4_2:28425728-28425747 Msa0596840:three_prime_UTR 25.0%
! AAAAAAAACCTGCATAATTG+TGG + chr4_2:28432186-28432205 None:intergenic 25.0%
! ATAACATTCATGGATAATTG+TGG + chr4_2:28425991-28426010 None:intergenic 25.0%
! ACAATTATCCATGAATGTTA+TGG - chr4_2:28425990-28426009 Msa0596840:intron 25.0%
! CAATTATCCATGAATGTTAT+GGG - chr4_2:28425991-28426010 Msa0596840:intron 25.0%
!!! TTTGATTTATTTTCCCTAAG+TGG - chr4_2:28426098-28426117 Msa0596840:intron 25.0%
! TTGTTTATAATTAGAAACGG+AGG - chr4_2:28432645-28432664 Msa0596840:five_prime_UTR 25.0%
! TGTTTATAATTAGAAACGGA+GGG - chr4_2:28432646-28432665 Msa0596840:five_prime_UTR 25.0%
TTTATATGTTCCAAAATCGG+TGG + chr4_2:28425451-28425470 None:intergenic 30.0%
! TACAATGGACTCAATACATA+TGG + chr4_2:28425477-28425496 None:intergenic 30.0%
!!! AAAGAAGTGCTCTCTTATTT+TGG - chr4_2:28425514-28425533 Msa0596840:three_prime_UTR 30.0%
! TCTGTTCAGTTTTTCATCAA+AGG - chr4_2:28425553-28425572 Msa0596840:three_prime_UTR 30.0%
! TTTTGGTACAGAAATTATGC+AGG - chr4_2:28425835-28425854 Msa0596840:exon 30.0%
!! TGTTAGGTTGGATCAATTTA+CGG - chr4_2:28426016-28426035 Msa0596840:intron 30.0%
TTGCTTATAATTAGAAACGG+AGG - chr4_2:28426275-28426294 Msa0596840:CDS 30.0%
TGCTTATAATTAGAAACGGA+GGG - chr4_2:28426276-28426295 Msa0596840:CDS 30.0%
TGAAAAGGAGCTTACATTTA+GGG + chr4_2:28426716-28426735 None:intergenic 30.0%
TTGAAAAGGAGCTTACATTT+AGG + chr4_2:28426717-28426736 None:intergenic 30.0%
TCAATGAGTGTCTACATTAA+GGG - chr4_2:28426918-28426937 Msa0596840:intron 30.0%
!! TGTTCATCTACTGATTTACA+TGG - chr4_2:28426995-28427014 Msa0596840:intron 30.0%
AGATTGATTAGGCTTAATCA+AGG + chr4_2:28427403-28427422 None:intergenic 30.0%
TTCTCTCAAGAAGATTGATT+AGG + chr4_2:28427414-28427433 None:intergenic 30.0%
! TCAATACTTTGAAAGTGAGA+AGG - chr4_2:28427570-28427589 Msa0596840:intron 30.0%
!!! TCGATTCAATCTGTTGTTTT+TGG + chr4_2:28427639-28427658 None:intergenic 30.0%
! ACTGTCTGAATTGATTCAAT+AGG + chr4_2:28427670-28427689 None:intergenic 30.0%
TAAAGACTAAATCCAGATGA+AGG - chr4_2:28427691-28427710 Msa0596840:intron 30.0%
ATCTTGTAACGGTTTAATTC+TGG + chr4_2:28427988-28428007 None:intergenic 30.0%
ATCATCAAAACTATAGCTAG+AGG - chr4_2:28428101-28428120 Msa0596840:intron 30.0%
AATAAGGAATATGGTGGTTT+CGG + chr4_2:28428310-28428329 None:intergenic 30.0%
TGTAAGAATAAGGAATATGG+TGG + chr4_2:28428316-28428335 None:intergenic 30.0%
! AAGAAGGAAAAAGCATAAAC+AGG - chr4_2:28428444-28428463 Msa0596840:intron 30.0%
AAGAATCACACTTTAACGTT+AGG + chr4_2:28428510-28428529 None:intergenic 30.0%
TGGTTCTTCATTGTAAGATT+GGG + chr4_2:28428635-28428654 None:intergenic 30.0%
ATGGTTCTTCATTGTAAGAT+TGG + chr4_2:28428636-28428655 None:intergenic 30.0%
TGATTCTTAGTTAAATGAGG+CGG + chr4_2:28428661-28428680 None:intergenic 30.0%
! TAATTTCCACAAGAGTATGT+TGG + chr4_2:28428700-28428719 None:intergenic 30.0%
! GAGTCAAATGCTTTTATACA+TGG + chr4_2:28429154-28429173 None:intergenic 30.0%
!! CGGATAATGTCTTTTTAAAG+TGG - chr4_2:28429268-28429287 Msa0596840:intron 30.0%
AGATGAAAAGAAATGCAAAG+GGG - chr4_2:28429466-28429485 Msa0596840:intron 30.0%
!!! TAGTGGTAGTTTTTTCTACA+AGG + chr4_2:28429685-28429704 None:intergenic 30.0%
ATTTAGACTGTAGGATTTAG+TGG + chr4_2:28429702-28429721 None:intergenic 30.0%
AAATAACTTCCTAAGACACT+AGG - chr4_2:28429731-28429750 Msa0596840:intron 30.0%
! AAGTTATTTCCTAGTGTCTT+AGG + chr4_2:28429743-28429762 None:intergenic 30.0%
TTTGTCGAAATAACCCATAT+GGG - chr4_2:28429944-28429963 Msa0596840:intron 30.0%
AAGAAAATGTAGATTGCCAT+AGG - chr4_2:28430056-28430075 Msa0596840:intron 30.0%
TAATTCAATGAAAGGAAGGT+TGG - chr4_2:28430211-28430230 Msa0596840:intron 30.0%
AGAAGGCTAACAAAAGAAAT+TGG + chr4_2:28430283-28430302 None:intergenic 30.0%
GGTTAAAGGTGGATATATTT+GGG + chr4_2:28430308-28430327 None:intergenic 30.0%
AGGTTAAAGGTGGATATATT+TGG + chr4_2:28430309-28430328 None:intergenic 30.0%
AATAATGTGGCTTAGGTTAA+AGG + chr4_2:28430322-28430341 None:intergenic 30.0%
! TAAAGTCAGTAGATTGGTTA+CGG + chr4_2:28430454-28430473 None:intergenic 30.0%
ATCATCTCAACATACATTTC+CGG + chr4_2:28430614-28430633 None:intergenic 30.0%
TATCAAAGAAGGTCATCATA+TGG + chr4_2:28431136-28431155 None:intergenic 30.0%
TGTTGTCATCTAAAAAGTTC+AGG + chr4_2:28431241-28431260 None:intergenic 30.0%
AACAAAATGTGGAAAACACT+TGG - chr4_2:28431257-28431276 Msa0596840:intron 30.0%
!!! ATTTACCCCACTTTTTTTAG+TGG - chr4_2:28431315-28431334 Msa0596840:intron 30.0%
!!! TTTACCCCACTTTTTTTAGT+GGG - chr4_2:28431316-28431335 Msa0596840:intron 30.0%
AACAACCCACTAAAAAAAGT+GGG + chr4_2:28431324-28431343 None:intergenic 30.0%
TAACAACCCACTAAAAAAAG+TGG + chr4_2:28431325-28431344 None:intergenic 30.0%
!!! TCTTTTTTAGTTCAAACGGT+TGG - chr4_2:28431581-28431600 Msa0596840:intron 30.0%
!! GATCTAGACTTTGATTTTTG+AGG + chr4_2:28431611-28431630 None:intergenic 30.0%
TTTATATGTTCCAAAATCGG+TGG + chr4_2:28425451-28425470 None:intergenic 30.0%
! TACAATGGACTCAATACATA+TGG + chr4_2:28425477-28425496 None:intergenic 30.0%
! TCTGTTCAGTTTTTCATCAA+AGG - chr4_2:28425553-28425572 Msa0596840:three_prime_UTR 30.0%
! TTTTGGTACAGAAATTATGC+AGG - chr4_2:28425835-28425854 Msa0596840:exon 30.0%
!! TGTTAGGTTGGATCAATTTA+CGG - chr4_2:28426016-28426035 Msa0596840:intron 30.0%
!!! AAATCAACAACCACCGATTT+TGG - chr4_2:28425438-28425457 Msa0596840:three_prime_UTR 35.0%
GTCCATTGTAGCAAGAAAAA+TGG - chr4_2:28425487-28425506 Msa0596840:three_prime_UTR 35.0%
!!! AGCCATTTTTCTTGCTACAA+TGG + chr4_2:28425492-28425511 None:intergenic 35.0%
!! TTTCATCAAAGGCATTAGCA+AGG - chr4_2:28425564-28425583 Msa0596840:three_prime_UTR 35.0%
GCGCAAATGCAATAACAAAA+TGG + chr4_2:28425644-28425663 None:intergenic 35.0%
ATAAGGAGTCTGATACAGTA+AGG - chr4_2:28425948-28425967 Msa0596840:intron 35.0%
!! TGATACAGTAAGGTGTTTTG+AGG - chr4_2:28425958-28425977 Msa0596840:intron 35.0%
GATCAATTTACGGATGAAGT+TGG - chr4_2:28426026-28426045 Msa0596840:CDS 35.0%
!! CTTTTTGTTTGCATCCTTGA+CGG + chr4_2:28426491-28426510 None:intergenic 35.0%
AGTATCTTTAATCAGTGCTC+CGG - chr4_2:28426602-28426621 Msa0596840:intron 35.0%
CAAATGGGTGCTTAGATATA+GGG + chr4_2:28426680-28426699 None:intergenic 35.0%
TCAAATGGGTGCTTAGATAT+AGG + chr4_2:28426681-28426700 None:intergenic 35.0%
GTCAATGAGTGTCTACATTA+AGG - chr4_2:28426917-28426936 Msa0596840:intron 35.0%
TCAATGAGCACCAAATTCAA+GGG + chr4_2:28427223-28427242 None:intergenic 35.0%
TTCAATGAGCACCAAATTCA+AGG + chr4_2:28427224-28427243 None:intergenic 35.0%
!! TTTGGTGCTCATTGAATCTT+CGG - chr4_2:28427228-28427247 Msa0596840:intron 35.0%
TGCTAATAGGGATTGTAAGT+TGG + chr4_2:28427437-28427456 None:intergenic 35.0%
TTGAGTGTGTAGATTCTCTA+GGG + chr4_2:28427460-28427479 None:intergenic 35.0%
TTTGAGTGTGTAGATTCTCT+AGG + chr4_2:28427461-28427480 None:intergenic 35.0%
!! GGAGAGGTGTTTATATAGTA+AGG - chr4_2:28427591-28427610 Msa0596840:intron 35.0%
! ATCGATTCAAACATCCATCT+GGG + chr4_2:28427770-28427789 None:intergenic 35.0%
! AATCGATTCAAACATCCATC+TGG + chr4_2:28427771-28427790 None:intergenic 35.0%
! TGGATGTTTGAATCGATTCA+AGG - chr4_2:28427773-28427792 Msa0596840:intron 35.0%
ATTCTGGTATCCTTTCGTTT+GGG + chr4_2:28427972-28427991 None:intergenic 35.0%
AATTCTGGTATCCTTTCGTT+TGG + chr4_2:28427973-28427992 None:intergenic 35.0%
ATAAGGAATATGGTGGTTTC+GGG + chr4_2:28428309-28428328 None:intergenic 35.0%
GGATCATAGGAAAATTGCAT+GGG + chr4_2:28428356-28428375 None:intergenic 35.0%
AGGATCATAGGAAAATTGCA+TGG + chr4_2:28428357-28428376 None:intergenic 35.0%
!! ACTTGAAAGAGCTTTGAAGA+AGG - chr4_2:28428428-28428447 Msa0596840:intron 35.0%
! AAAGCATAAACAGGTTGAGA+AGG - chr4_2:28428453-28428472 Msa0596840:intron 35.0%
! CATACTTTAGGAGGATTAAG+CGG - chr4_2:28428481-28428500 Msa0596840:intron 35.0%
TCTTCATTGTAAGATTGGGA+GGG + chr4_2:28428631-28428650 None:intergenic 35.0%
TTCTTCATTGTAAGATTGGG+AGG + chr4_2:28428632-28428651 None:intergenic 35.0%
! TTCTTTTTGACGCTATGTTG+GGG + chr4_2:28428731-28428750 None:intergenic 35.0%
! GTTCTTTTTGACGCTATGTT+GGG + chr4_2:28428732-28428751 None:intergenic 35.0%
! TGTTCTTTTTGACGCTATGT+TGG + chr4_2:28428733-28428752 None:intergenic 35.0%
AGCGTCAAAAAGAACAAGAA+CGG - chr4_2:28428737-28428756 Msa0596840:intron 35.0%
TATACCCTGTGAACATGTTA+GGG - chr4_2:28428862-28428881 Msa0596840:intron 35.0%
ATTTGCTCATTAATCCTACG+TGG - chr4_2:28428934-28428953 Msa0596840:intron 35.0%
TGGAGAAGAATGCAAGAAAT+TGG + chr4_2:28429088-28429107 None:intergenic 35.0%
ATTCGTCAATGGTTTATGAC+CGG - chr4_2:28429248-28429267 Msa0596840:intron 35.0%
AATTGCAGGAATGTAAATGG+TGG - chr4_2:28429512-28429531 Msa0596840:intron 35.0%
AACCATATGCATGCAACATT+TGG - chr4_2:28429860-28429879 Msa0596840:intron 35.0%
AACCAAATGTTGCATGCATA+TGG + chr4_2:28429865-28429884 None:intergenic 35.0%
GCAACATTTGGTTGAAATTC+TGG - chr4_2:28429872-28429891 Msa0596840:intron 35.0%
GTTTGTCGAAATAACCCATA+TGG - chr4_2:28429943-28429962 Msa0596840:intron 35.0%
ATTTCGACAACAACCCATAT+GGG + chr4_2:28429960-28429979 None:intergenic 35.0%
GGAGTTAGATTATCAACCTA+AGG + chr4_2:28430262-28430281 None:intergenic 35.0%
GTGGATATATTTGGGAAAGA+AGG + chr4_2:28430300-28430319 None:intergenic 35.0%
TCAGGAAAATAATGTGGCTT+AGG + chr4_2:28430329-28430348 None:intergenic 35.0%
GGTCTTTCAGGAAAATAATG+TGG + chr4_2:28430335-28430354 None:intergenic 35.0%
!! GTAACCAATCTACTGACTTT+AGG - chr4_2:28430453-28430472 Msa0596840:intron 35.0%
ATCTCCTAAAGTCAGTAGAT+TGG + chr4_2:28430460-28430479 None:intergenic 35.0%
CAACATTGATGAACTTACCT+CGG + chr4_2:28430558-28430577 None:intergenic 35.0%
TCATCTCAACATACATTTCC+GGG + chr4_2:28430613-28430632 None:intergenic 35.0%
! ATTTTTCTCAAACATCGCAG+CGG + chr4_2:28430669-28430688 None:intergenic 35.0%
CAAATACCTCTGTAAAGTGT+TGG - chr4_2:28431073-28431092 Msa0596840:intron 35.0%
CTAAAAAGTTCAGGAGAGTT+CGG + chr4_2:28431232-28431251 None:intergenic 35.0%
!! TATGCTTTTGACTACTCGTA+TGG - chr4_2:28431289-28431308 Msa0596840:intron 35.0%
!! ATGCTTTTGACTACTCGTAT+GGG - chr4_2:28431290-28431309 Msa0596840:intron 35.0%
ACAACCCACTAAAAAAAGTG+GGG + chr4_2:28431323-28431342 None:intergenic 35.0%
AATGATTATATAGAGGCCCT+TGG + chr4_2:28431639-28431658 None:intergenic 35.0%
!!! AAATCAACAACCACCGATTT+TGG - chr4_2:28425438-28425457 Msa0596840:three_prime_UTR 35.0%
GTCCATTGTAGCAAGAAAAA+TGG - chr4_2:28425487-28425506 Msa0596840:three_prime_UTR 35.0%
!!! AGCCATTTTTCTTGCTACAA+TGG + chr4_2:28425492-28425511 None:intergenic 35.0%
!!! AAAGAAGTGCTCTCTTGTTT+TGG - chr4_2:28431891-28431910 Msa0596840:intron 35.0%
!! TTTCATCAAAGGCATTAGCA+AGG - chr4_2:28425564-28425583 Msa0596840:three_prime_UTR 35.0%
GCGCAAATGCAATAACAAAA+TGG + chr4_2:28425644-28425663 None:intergenic 35.0%
ATAAGGAGTCTGATACAGTA+AGG - chr4_2:28425948-28425967 Msa0596840:intron 35.0%
!! TGATACAGTAAGGTGTTTTG+AGG - chr4_2:28425958-28425977 Msa0596840:intron 35.0%
GATCAATTTACGGATGAAGT+TGG - chr4_2:28426026-28426045 Msa0596840:CDS 35.0%
TATGGGGAAGTTCACTTACA+TGG + chr4_2:28425617-28425636 None:intergenic 40.0%
TAACACGCCCATAACATTCA+TGG + chr4_2:28426001-28426020 None:intergenic 40.0%
ATGAATGTTATGGGCGTGTT+AGG - chr4_2:28426000-28426019 Msa0596840:intron 40.0%
TACACAAGGGTCATGTAAAC+CGG - chr4_2:28426452-28426471 Msa0596840:intron 40.0%
GTATCTTTAATCAGTGCTCC+GGG - chr4_2:28426603-28426622 Msa0596840:intron 40.0%
TATCTTTAATCAGTGCTCCG+GGG - chr4_2:28426604-28426623 Msa0596840:intron 40.0%
TGAGTGTCTACATTAAGGGA+CGG - chr4_2:28426922-28426941 Msa0596840:intron 40.0%
!! TCAAGGGGTACTTGAAGTTT+GGG + chr4_2:28427207-28427226 None:intergenic 40.0%
!! TTCAAGGGGTACTTGAAGTT+TGG + chr4_2:28427208-28427227 None:intergenic 40.0%
CTTCAAGTACCCCTTGAATT+TGG - chr4_2:28427210-28427229 Msa0596840:intron 40.0%
CAATGAGCACCAAATTCAAG+GGG + chr4_2:28427222-28427241 None:intergenic 40.0%
TAACCGTTCTTGAAGTCGAA+GGG + chr4_2:28427326-28427345 None:intergenic 40.0%
TAATCTCATTCCACCTCCAA+AGG + chr4_2:28427364-28427383 None:intergenic 40.0%
AGGCTTAATCAAGGCTTCAA+AGG + chr4_2:28427394-28427413 None:intergenic 40.0%
!! GATTCTCTAGGGTGCTAATA+GGG + chr4_2:28427449-28427468 None:intergenic 40.0%
!! AGATTCTCTAGGGTGCTAAT+AGG + chr4_2:28427450-28427469 None:intergenic 40.0%
! ACTTTGAAAGTGAGAAGGAG+AGG - chr4_2:28427575-28427594 Msa0596840:intron 40.0%
TCCTAGTGAGCATCTTGTAA+CGG + chr4_2:28427999-28428018 None:intergenic 40.0%
TGTATGTTCTTCCTTGTGGA+GGG + chr4_2:28428145-28428164 None:intergenic 40.0%
!! GATCTAGTTTAAGGTGTTGG+CGG + chr4_2:28428212-28428231 None:intergenic 40.0%
!! AGGTTGTTAGTTGATAGTGG+GGG + chr4_2:28428235-28428254 None:intergenic 40.0%
!! GAGGTTGTTAGTTGATAGTG+GGG + chr4_2:28428236-28428255 None:intergenic 40.0%
!! CGAGGTTGTTAGTTGATAGT+GGG + chr4_2:28428237-28428256 None:intergenic 40.0%
!! TCGAGGTTGTTAGTTGATAG+TGG + chr4_2:28428238-28428257 None:intergenic 40.0%
TGTTAGGAAAATGGTGTTCG+AGG + chr4_2:28428255-28428274 None:intergenic 40.0%
!!! ATTTTTTTAGGGTGGGTGTG+AGG + chr4_2:28428377-28428396 None:intergenic 40.0%
! CATAAACAGGTTGAGAAGGT+AGG - chr4_2:28428457-28428476 Msa0596840:intron 40.0%
GAGAAGGTAGGACATACTTT+AGG - chr4_2:28428469-28428488 Msa0596840:intron 40.0%
AAGGTAGGACATACTTTAGG+AGG - chr4_2:28428472-28428491 Msa0596840:intron 40.0%
! GTTGTCTTGGGATCCAATTT+TGG + chr4_2:28428562-28428581 None:intergenic 40.0%
!! AGGGTTCCACTTTTGTACTT+GGG + chr4_2:28428612-28428631 None:intergenic 40.0%
TTAGTTAAATGAGGCGGCAA+TGG + chr4_2:28428655-28428674 None:intergenic 40.0%
! TTCCACAAGAGTATGTTGGT+GGG + chr4_2:28428696-28428715 None:intergenic 40.0%
! TTTCCACAAGAGTATGTTGG+TGG + chr4_2:28428697-28428716 None:intergenic 40.0%
TGATGATACCTTGTTCATGG+GGG + chr4_2:28428796-28428815 None:intergenic 40.0%
CTGATGATACCTTGTTCATG+GGG + chr4_2:28428797-28428816 None:intergenic 40.0%
GCTGATGATACCTTGTTCAT+GGG + chr4_2:28428798-28428817 None:intergenic 40.0%
TGCTGATGATACCTTGTTCA+TGG + chr4_2:28428799-28428818 None:intergenic 40.0%
CTATACCCTGTGAACATGTT+AGG - chr4_2:28428861-28428880 Msa0596840:intron 40.0%
GAAACCCTAACATGTTCACA+GGG + chr4_2:28428869-28428888 None:intergenic 40.0%
TGAAACCCTAACATGTTCAC+AGG + chr4_2:28428870-28428889 None:intergenic 40.0%
GCAAATTTACTCAACCATCG+TGG - chr4_2:28428959-28428978 Msa0596840:intron 40.0%
TGAAGATGCTGGAAAAATCG+TGG + chr4_2:28429005-28429024 None:intergenic 40.0%
! ACGATTTTTCCAGCATCTTC+AGG - chr4_2:28429004-28429023 Msa0596840:intron 40.0%
TGGAATTTACCTGAAGATGC+TGG + chr4_2:28429016-28429035 None:intergenic 40.0%
TTCAAAACGTGCCACGATTT+AGG - chr4_2:28429039-28429058 Msa0596840:intron 40.0%
GTTACAGTTGGAGTGCAATA+AGG + chr4_2:28429808-28429827 None:intergenic 40.0%
GACAAACTGCAAAGTGCAAA+GGG + chr4_2:28429930-28429949 None:intergenic 40.0%
GATTTCGACAACAACCCATA+TGG + chr4_2:28429961-28429980 None:intergenic 40.0%
AATGTGGCTTAGGTTAAAGG+TGG + chr4_2:28430319-28430338 None:intergenic 40.0%
!! TACACACTTTGAGGTCTTTC+AGG + chr4_2:28430347-28430366 None:intergenic 40.0%
ATTCATGTGACAACTGGAGT+GGG - chr4_2:28430422-28430441 Msa0596840:intron 40.0%
! TTCAGGAGAGTTCGGTTTTA+GGG + chr4_2:28431224-28431243 None:intergenic 40.0%
GTGATTGTACAATTCTGCGT+TGG - chr4_2:28431363-28431382 Msa0596840:intron 40.0%
AAAGTCTAGATCCCAATCCA+AGG - chr4_2:28431619-28431638 Msa0596840:intron 40.0%
AAGTCTAGATCCCAATCCAA+GGG - chr4_2:28431620-28431639 Msa0596840:intron 40.0%
TATATAGAGGCCCTTGGATT+GGG + chr4_2:28431633-28431652 None:intergenic 40.0%
TTATATAGAGGCCCTTGGAT+TGG + chr4_2:28431634-28431653 None:intergenic 40.0%
TATGGGGAAGTTCACTTACA+TGG + chr4_2:28425617-28425636 None:intergenic 40.0%
TAACACGCCCATAACATTCA+TGG + chr4_2:28426001-28426020 None:intergenic 40.0%
ATGAATGTTATGGGCGTGTT+AGG - chr4_2:28426000-28426019 Msa0596840:intron 40.0%
!!! GTAAGGTGTTTTGAGGCATG+TGG - chr4_2:28425965-28425984 Msa0596840:intron 45.0%
ATGTTATGGGCGTGTTAGGT+TGG - chr4_2:28426004-28426023 Msa0596840:intron 45.0%
ACACTCGAGCTTTCCACTTA+GGG + chr4_2:28426114-28426133 None:intergenic 45.0%
AACACTCGAGCTTTCCACTT+AGG + chr4_2:28426115-28426134 None:intergenic 45.0%
TGCTTAGATATAGGGACGGA+TGG + chr4_2:28426672-28426691 None:intergenic 45.0%
TGGGTGCTTAGATATAGGGA+CGG + chr4_2:28426676-28426695 None:intergenic 45.0%
TGTCTACATTAAGGGACGGA+GGG - chr4_2:28426926-28426945 Msa0596840:intron 45.0%
!! AAGGGGTACTTGAAGTTTGG+GGG + chr4_2:28427205-28427224 None:intergenic 45.0%
!! CAAGGGGTACTTGAAGTTTG+GGG + chr4_2:28427206-28427225 None:intergenic 45.0%
AACCGTTCTTGAAGTCGAAG+GGG + chr4_2:28427325-28427344 None:intergenic 45.0%
CTAACCGTTCTTGAAGTCGA+AGG + chr4_2:28427327-28427346 None:intergenic 45.0%
ATTCCACCTCCAAAGGACTT+GGG + chr4_2:28427357-28427376 None:intergenic 45.0%
AGACACAAGACCCAAACGAA+AGG - chr4_2:28427959-28427978 Msa0596840:intron 45.0%
ACCGTTACAAGATGCTCACT+AGG - chr4_2:28427995-28428014 Msa0596840:intron 45.0%
GTGTATGTTCTTCCTTGTGG+AGG + chr4_2:28428146-28428165 None:intergenic 45.0%
! GGGGATCTAGTTTAAGGTGT+TGG + chr4_2:28428215-28428234 None:intergenic 45.0%
! TAGTGGGGGGATCTAGTTTA+AGG + chr4_2:28428221-28428240 None:intergenic 45.0%
!! GGTTGTTAGTTGATAGTGGG+GGG + chr4_2:28428234-28428253 None:intergenic 45.0%
AATATGGTGGTTTCGGGCTT+CGG + chr4_2:28428303-28428322 None:intergenic 45.0%
TTGTACTTGGGTTTGCCGAT+TGG + chr4_2:28428600-28428619 None:intergenic 45.0%
GGCAAACCCAAGTACAAAAG+TGG - chr4_2:28428603-28428622 Msa0596840:intron 45.0%
!! GAGGGTTCCACTTTTGTACT+TGG + chr4_2:28428613-28428632 None:intergenic 45.0%
! TCCACAAGAGTATGTTGGTG+GGG + chr4_2:28428695-28428714 None:intergenic 45.0%
AAGAACAAGAACGGCTCGTA+TGG - chr4_2:28428746-28428765 Msa0596840:intron 45.0%
!! GAGTAAATTTGCTGCCACGT+AGG + chr4_2:28428951-28428970 None:intergenic 45.0%
GAGAAGAAAACATGCCACGA+TGG + chr4_2:28429066-28429085 None:intergenic 45.0%
GCAGGAATGTAAATGGTGGT+TGG - chr4_2:28429516-28429535 Msa0596840:intron 45.0%
AGGCAAAGTAGAACGTGTAG+CGG + chr4_2:28429788-28429807 None:intergenic 45.0%
CGACAAACTGCAAAGTGCAA+AGG + chr4_2:28429931-28429950 None:intergenic 45.0%
CATTCATGTGACAACTGGAG+TGG - chr4_2:28430421-28430440 Msa0596840:intron 45.0%
GGAGATACATACGTCTCTCA+TGG - chr4_2:28430474-28430493 Msa0596840:intron 45.0%
! GTCTCTCATGGAAAAGTGTG+TGG - chr4_2:28430486-28430505 Msa0596840:intron 45.0%
ACACTCAAACGGGATAGACT+TGG + chr4_2:28430534-28430553 None:intergenic 45.0%
TTACCTCGGTACACTCAAAC+GGG + chr4_2:28430544-28430563 None:intergenic 45.0%
CTTACCTCGGTACACTCAAA+CGG + chr4_2:28430545-28430564 None:intergenic 45.0%
! TCTTGGGGCTTTATGCTTGT+TGG - chr4_2:28430817-28430836 Msa0596840:intron 45.0%
GGTGAACCAACACTTTACAG+AGG + chr4_2:28431082-28431101 None:intergenic 45.0%
TTGAACCCCAACAATCTTGC+GGG + chr4_2:28431103-28431122 None:intergenic 45.0%
GTTGAACCCCAACAATCTTG+CGG + chr4_2:28431104-28431123 None:intergenic 45.0%
!! TCAGGAGAGTTCGGTTTTAG+GGG + chr4_2:28431223-28431242 None:intergenic 45.0%
! GTTCAGGAGAGTTCGGTTTT+AGG + chr4_2:28431225-28431244 None:intergenic 45.0%
!!! GTAAGGTGTTTTGAGGCATG+TGG - chr4_2:28425965-28425984 Msa0596840:intron 45.0%
ATGTTATGGGCGTGTTAGGT+TGG - chr4_2:28426004-28426023 Msa0596840:intron 45.0%
ACACTCGAGCTTTCCACTTA+GGG + chr4_2:28426114-28426133 None:intergenic 45.0%
AACACTCGAGCTTTCCACTT+AGG + chr4_2:28426115-28426134 None:intergenic 45.0%
!!! TCTTTTTAATAATTTTTTTA+GGG + chr4_2:28428388-28428407 None:intergenic 5.0%
!! AATACTAAAAATAAAATATT+TGG + chr4_2:28429999-28430018 None:intergenic 5.0%
!!! ATATTAGTAATTTATTTTAT+GGG + chr4_2:28431009-28431028 None:intergenic 5.0%
!!! AATATTAGTAATTTATTTTA+TGG + chr4_2:28431010-28431029 None:intergenic 5.0%
TTGTGGCCACGTGCATTATC+AGG + chr4_2:28425794-28425813 None:intergenic 50.0%
! TGGAAAGCTCGAGTGTTGCA+CGG - chr4_2:28426118-28426137 Msa0596840:intron 50.0%
GAAAATGTTAACCGGTGCCC+CGG + chr4_2:28426624-28426643 None:intergenic 50.0%
GTGTCTACATTAAGGGACGG+AGG - chr4_2:28426925-28426944 Msa0596840:intron 50.0%
! AGGAGTGCACGGGTAATTCA+AGG + chr4_2:28427256-28427275 None:intergenic 50.0%
! AAGAGTTCGAAGGAGTGCAC+GGG + chr4_2:28427266-28427285 None:intergenic 50.0%
! GAAGAGTTCGAAGGAGTGCA+CGG + chr4_2:28427267-28427286 None:intergenic 50.0%
GACTCGAGTGAAGAGTTCGA+AGG + chr4_2:28427276-28427295 None:intergenic 50.0%
CGAGTCTTCAATGCTACGTC+CGG - chr4_2:28427290-28427309 Msa0596840:intron 50.0%
ACCCCCTTCGACTTCAAGAA+CGG - chr4_2:28427320-28427339 Msa0596840:intron 50.0%
ACCGTTCTTGAAGTCGAAGG+GGG + chr4_2:28427324-28427343 None:intergenic 50.0%
AGAAAGACCCCCAAGTCCTT+TGG - chr4_2:28427345-28427364 Msa0596840:intron 50.0%
TTCCACCTCCAAAGGACTTG+GGG + chr4_2:28427356-28427375 None:intergenic 50.0%
CATTCCACCTCCAAAGGACT+TGG + chr4_2:28427358-28427377 None:intergenic 50.0%
AACGATTCAAGCTGCCCAGA+TGG - chr4_2:28427753-28427772 Msa0596840:intron 50.0%
ATGTTCTTCCTTGTGGAGGG+AGG + chr4_2:28428142-28428161 None:intergenic 50.0%
GGCGTGTATGTTCTTCCTTG+TGG + chr4_2:28428149-28428168 None:intergenic 50.0%
! TGATGCTCGTCGGTTGTCTT+GGG + chr4_2:28428574-28428593 None:intergenic 50.0%
! TACTTGGGTTTGCCGATTGG+TGG + chr4_2:28428597-28428616 None:intergenic 50.0%
TCCCCACCAACATACTCTTG+TGG - chr4_2:28428691-28428710 Msa0596840:intron 50.0%
TGACGCTATGTTGGGGATGA+AGG + chr4_2:28428724-28428743 None:intergenic 50.0%
GTTCATGGGGGTCAAGAGTT+GGG + chr4_2:28428784-28428803 None:intergenic 50.0%
TGTTCATGGGGGTCAAGAGT+TGG + chr4_2:28428785-28428804 None:intergenic 50.0%
ACTCTTGACCCCCATGAACA+AGG - chr4_2:28428785-28428804 Msa0596840:intron 50.0%
TCAAAACGTGGCACGATGGT+GGG + chr4_2:28428899-28428918 None:intergenic 50.0%
TTCAAAACGTGGCACGATGG+TGG + chr4_2:28428900-28428919 None:intergenic 50.0%
GGTTTCAAAACGTGGCACGA+TGG + chr4_2:28428903-28428922 None:intergenic 50.0%
!!! GCCACGTTTTGAAACCATCG+TGG - chr4_2:28428907-28428926 Msa0596840:intron 50.0%
GCCACGATGGTTTCAAAACG+TGG + chr4_2:28428911-28428930 None:intergenic 50.0%
ATGAGCAAATCGTGCCACGA+TGG + chr4_2:28428924-28428943 None:intergenic 50.0%
CTGGAAAAATCGTGGCACGA+TGG + chr4_2:28428997-28429016 None:intergenic 50.0%
! CAAACGGGTTCAAGAGTGGA+TGG + chr4_2:28429553-28429572 None:intergenic 50.0%
AGAACGTGTAGCGGCAAACA+AGG + chr4_2:28429779-28429798 None:intergenic 50.0%
CGTCGAAGTCTGTATGCTCT+TGG - chr4_2:28429897-28429916 Msa0596840:intron 50.0%
CTCAAACGGGATAGACTTGG+AGG + chr4_2:28430531-28430550 None:intergenic 50.0%
TATCCCGTTTGAGTGTACCG+AGG - chr4_2:28430538-28430557 Msa0596840:intron 50.0%
! GATGTTCTGAGCACAACTCC+CGG - chr4_2:28430592-28430611 Msa0596840:intron 50.0%
AGATCCGAGAGACGTGTGAA+GGG + chr4_2:28430800-28430819 None:intergenic 50.0%
AAGATCCGAGAGACGTGTGA+AGG + chr4_2:28430801-28430820 None:intergenic 50.0%
TTCACACGTCTCTCGGATCT+TGG - chr4_2:28430800-28430819 Msa0596840:intron 50.0%
TCACACGTCTCTCGGATCTT+GGG - chr4_2:28430801-28430820 Msa0596840:intron 50.0%
! GCTTGTTGGTCTGTCTTTGC+AGG - chr4_2:28430831-28430850 Msa0596840:intron 50.0%
! TGTTGGTCTGTCTTTGCAGG+AGG - chr4_2:28430834-28430853 Msa0596840:intron 50.0%
TGGTTCACCCGCAAGATTGT+TGG - chr4_2:28431093-28431112 Msa0596840:intron 50.0%
GGTTCACCCGCAAGATTGTT+GGG - chr4_2:28431094-28431113 Msa0596840:intron 50.0%
GTTCACCCGCAAGATTGTTG+GGG - chr4_2:28431095-28431114 Msa0596840:intron 50.0%
TTGTGGCCACGTGCATTATC+AGG + chr4_2:28425794-28425813 None:intergenic 50.0%
GTCATGTAAACCGGTGCCCT+CGG - chr4_2:28426461-28426480 Msa0596840:intron 55.0%
TCATGTAAACCGGTGCCCTC+GGG - chr4_2:28426462-28426481 Msa0596840:intron 55.0%
AAGACCCCCAAGTCCTTTGG+AGG - chr4_2:28427348-28427367 Msa0596840:intron 55.0%
TCCACCTCCAAAGGACTTGG+GGG + chr4_2:28427355-28427374 None:intergenic 55.0%
CTTCCTTGTGGAGGGAGGTA+GGG + chr4_2:28428137-28428156 None:intergenic 55.0%
TCTTCCTTGTGGAGGGAGGT+AGG + chr4_2:28428138-28428157 None:intergenic 55.0%
!! AGTTTAAGGTGTTGGCGGCC+CGG + chr4_2:28428207-28428226 None:intergenic 55.0%
TTTCGGGCTTCGGCGAATGA+AGG + chr4_2:28428293-28428312 None:intergenic 55.0%
AGGGTGGGTGTGAGGATCAT+AGG + chr4_2:28428369-28428388 None:intergenic 55.0%
! GTGATGCTCGTCGGTTGTCT+TGG + chr4_2:28428575-28428594 None:intergenic 55.0%
!! CGATTGGTGGTGATGCTCGT+CGG + chr4_2:28428584-28428603 None:intergenic 55.0%
CGACGAGCATCACCACCAAT+CGG - chr4_2:28428582-28428601 Msa0596840:intron 55.0%
GGATGTGTAACGTGCCACGA+TGG + chr4_2:28428976-28428995 None:intergenic 55.0%
!!! TCGTGGCACGTTTTGAAGCG+TGG + chr4_2:28429036-28429055 None:intergenic 55.0%
GCCACGATTTAGGTCCATCG+TGG - chr4_2:28429049-28429068 Msa0596840:intron 55.0%
GCCACGATGGACCTAAATCG+TGG + chr4_2:28429053-28429072 None:intergenic 55.0%
! GGAGCAAACGGGTTCAAGAG+TGG + chr4_2:28429557-28429576 None:intergenic 55.0%
GCAAACAAGGCTGGAGTGCA+TGG + chr4_2:28429766-28429785 None:intergenic 55.0%
CGGGATAGACTTGGAGGAAG+TGG + chr4_2:28430525-28430544 None:intergenic 55.0%
ACTCCCCTTCACACGTCTCT+CGG - chr4_2:28430793-28430812 Msa0596840:intron 55.0%
GATCCGAGAGACGTGTGAAG+GGG + chr4_2:28430799-28430818 None:intergenic 55.0%
CACACGTCTCTCGGATCTTG+GGG - chr4_2:28430802-28430821 Msa0596840:intron 55.0%
TGGTCTGTCTTTGCAGGAGG+AGG - chr4_2:28430837-28430856 Msa0596840:intron 55.0%
GCACGTGGCCACAATTATGC+AGG - chr4_2:28425800-28425819 Msa0596840:three_prime_UTR 55.0%
GCGCAGGCTTCTCACTAGCA+AGG + chr4_2:28425772-28425791 None:intergenic 60.0%
CCACGTGCATTATCAGGCGC+AGG + chr4_2:28425788-28425807 None:intergenic 60.0%
! CCTGCGCCTGATAATGCACG+TGG - chr4_2:28425785-28425804 Msa0596840:three_prime_UTR 60.0%
CATGTAAACCGGTGCCCTCG+GGG - chr4_2:28426463-28426482 Msa0596840:intron 60.0%
CATCCTTGACGGTACCCCGA+GGG + chr4_2:28426480-28426499 None:intergenic 60.0%
TAATCAGTGCTCCGGGGCAC+CGG - chr4_2:28426610-28426629 Msa0596840:intron 60.0%
GTCGAAGGGGGTTCGTTTGC+CGG + chr4_2:28427312-28427331 None:intergenic 60.0%
! ACCCCCAAGTCCTTTGGAGG+TGG - chr4_2:28427351-28427370 Msa0596840:intron 60.0%
TATGGTGTCTCAGGGGGTGG+GGG + chr4_2:28428184-28428203 None:intergenic 60.0%
GTATGGTGTCTCAGGGGGTG+GGG + chr4_2:28428185-28428204 None:intergenic 60.0%
GGTATGGTGTCTCAGGGGGT+GGG + chr4_2:28428186-28428205 None:intergenic 60.0%
CACCCCCTGAGACACCATAC+CGG - chr4_2:28428185-28428204 Msa0596840:intron 60.0%
ACCCCCTGAGACACCATACC+GGG - chr4_2:28428186-28428205 Msa0596840:intron 60.0%
CCACACGTCGTGGAGCAAAC+GGG + chr4_2:28429568-28429587 None:intergenic 60.0%
CCCACACGTCGTGGAGCAAA+CGG + chr4_2:28429569-28429588 None:intergenic 60.0%
CCGTTTGCTCCACGACGTGT+GGG - chr4_2:28429566-28429585 Msa0596840:intron 60.0%
ACGCTAACACCCACACGTCG+TGG + chr4_2:28429578-28429597 None:intergenic 60.0%
CGTGTAGCGGCAAACAAGGC+TGG + chr4_2:28429775-28429794 None:intergenic 60.0%
GCGCAGGCTTCTCACTAGCA+AGG + chr4_2:28425772-28425791 None:intergenic 60.0%
CCACGTGCATTATCAGGCGC+AGG + chr4_2:28425788-28425807 None:intergenic 60.0%
! CCTGCGCCTGATAATGCACG+TGG - chr4_2:28425785-28425804 Msa0596840:three_prime_UTR 60.0%
GCATCCTTGACGGTACCCCG+AGG + chr4_2:28426481-28426500 None:intergenic 65.0%
CGGTATGGTGTCTCAGGGGG+TGG + chr4_2:28428187-28428206 None:intergenic 65.0%
GCCCGGTATGGTGTCTCAGG+GGG + chr4_2:28428190-28428209 None:intergenic 65.0%
GGCCCGGTATGGTGTCTCAG+GGG + chr4_2:28428191-28428210 None:intergenic 65.0%
CGGCCCGGTATGGTGTCTCA+GGG + chr4_2:28428192-28428211 None:intergenic 65.0%
!! AAGGTGTTGGCGGCCCGGTA+TGG + chr4_2:28428202-28428221 None:intergenic 65.0%
CCCGTTTGCTCCACGACGTG+TGG - chr4_2:28429565-28429584 Msa0596840:intron 65.0%
! TGACGGTACCCCGAGGGCAC+CGG + chr4_2:28426474-28426493 None:intergenic 70.0%
!! GTGCCCTCGGGGTACCGTCA+AGG - chr4_2:28426474-28426493 Msa0596840:intron 70.0%
GCGCCCTACCTCCCTCCACA+AGG - chr4_2:28428131-28428150 Msa0596840:intron 70.0%
! AGGGGGTGGGGGTGCATCAG+GGG + chr4_2:28428173-28428192 None:intergenic 70.0%
! CAGGGGGTGGGGGTGCATCA+GGG + chr4_2:28428174-28428193 None:intergenic 70.0%
! TCAGGGGGTGGGGGTGCATC+AGG + chr4_2:28428175-28428194 None:intergenic 70.0%
GCGGCCCGGTATGGTGTCTC+AGG + chr4_2:28428193-28428212 None:intergenic 70.0%
! GGGTGGGGGTGCATCAGGGG+GGG + chr4_2:28428170-28428189 None:intergenic 75.0%
! GGGGTGGGGGTGCATCAGGG+GGG + chr4_2:28428171-28428190 None:intergenic 75.0%
! GGGGGTGGGGGTGCATCAGG+GGG + chr4_2:28428172-28428191 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 28425420 28432795 28425420 ID=Msa0596840;Name=Msa0596840
chr4_2 mRNA 28425420 28432795 28425420 ID=Msa0596840-mRNA-1;Parent=Msa0596840;Name=Msa0596840-mRNA-1;_AED=0.23;_eAED=0.23;_QI=348|1|1|1|0.8|0.66|6|422|91
chr4_2 exon 28425420 28425918 28425420 ID=Msa0596840-mRNA-1:exon:7330;Parent=Msa0596840-mRNA-1
chr4_2 exon 28426019 28426105 28426019 ID=Msa0596840-mRNA-1:exon:7329;Parent=Msa0596840-mRNA-1
chr4_2 exon 28426221 28426340 28426221 ID=Msa0596840-mRNA-1:exon:7328;Parent=Msa0596840-mRNA-1
chr4_2 exon 28432234 28432288 28432234 ID=Msa0596840-mRNA-1:exon:7327;Parent=Msa0596840-mRNA-1
chr4_2 exon 28432394 28432480 28432394 ID=Msa0596840-mRNA-1:exon:7326;Parent=Msa0596840-mRNA-1
chr4_2 exon 28432598 28432795 28432598 ID=Msa0596840-mRNA-1:exon:7325;Parent=Msa0596840-mRNA-1
chr4_2 five_prime_UTR 28432598 28432795 28432598 ID=Msa0596840-mRNA-1:five_prime_utr;Parent=Msa0596840-mRNA-1
chr4_2 five_prime_UTR 28432394 28432480 28432394 ID=Msa0596840-mRNA-1:five_prime_utr;Parent=Msa0596840-mRNA-1
chr4_2 five_prime_UTR 28432234 28432288 28432234 ID=Msa0596840-mRNA-1:five_prime_utr;Parent=Msa0596840-mRNA-1
chr4_2 five_prime_UTR 28426333 28426340 28426333 ID=Msa0596840-mRNA-1:five_prime_utr;Parent=Msa0596840-mRNA-1
chr4_2 CDS 28426221 28426332 28426221 ID=Msa0596840-mRNA-1:cds;Parent=Msa0596840-mRNA-1
chr4_2 CDS 28426019 28426105 28426019 ID=Msa0596840-mRNA-1:cds;Parent=Msa0596840-mRNA-1
chr4_2 CDS 28425842 28425918 28425842 ID=Msa0596840-mRNA-1:cds;Parent=Msa0596840-mRNA-1
chr4_2 three_prime_UTR 28425420 28425841 28425420 ID=Msa0596840-mRNA-1:three_prime_utr;Parent=Msa0596840-mRNA-1
Gene Sequence

>Msa0596840

ATGGCTTCAAAGAAGTGCTCTCTTGTTTTGGTAGTTCTCTTTATTTATCTGTTCAGTTTTTCATCAAAGGCATTAGCAAGGAACATTGCGAATGCTTCGAAACTTTATCCATTTTCAGATGAAATAGGTATGAATAAGAAGAGACTCCTTGCTAGTGAGAAGCCTGCGCCTGATAATGCACGTGGCCACAATTATGCAGTTGCAGAAGAAATAAATCTGAATAAGGAGTCTGATACAGTAAGGTGTTTTGAGGCATGTGGCCACAATTATCCATGA
Protein sequence

>Msa0596840

MASKKCSLVLVVLFIYLFSFSSKALARNIANASKLYPFSDEIGMNKKRLLASEKPAPDNARGHNYAVAEEINLNKESDTVRCFEACGHNYP*