Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0972680 | XP_039682720.1 | 95.270 | 148 | 7 | 0 | 1 | 148 | 1 | 148 | 4.44e-99 | 291 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0972680 | sp|P64956|Y2253_MYCBO | 45.600 | 125 | 67 | 1 | 11 | 134 | 3 | 127 | 2.11e-26 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0972680 | A0A396JEU9 | 93.919 | 148 | 9 | 0 | 1 | 148 | 1 | 148 | 1.60e-97 | 286 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017670 | Msa0972680 | 0.803697 | 3.079901e-49 | -8.615850e-47 |
| Msa0081020 | Msa0972680 | 0.803321 | 3.686627e-49 | -8.615850e-47 |
| Msa0610690 | Msa0972680 | 0.804185 | 2.439222e-49 | -8.615850e-47 |
| Msa0619780 | Msa0972680 | 0.848730 | 5.086289e-60 | -8.615850e-47 |
| Msa0657710 | Msa0972680 | 0.827365 | 1.628470e-54 | -8.615850e-47 |
| Msa0657720 | Msa0972680 | 0.837069 | 6.460881e-57 | -8.615850e-47 |
| Msa0674480 | Msa0972680 | 0.811196 | 7.900633e-51 | -8.615850e-47 |
| Msa0702790 | Msa0972680 | 0.835537 | 1.584021e-56 | -8.615850e-47 |
| Msa0702800 | Msa0972680 | 0.829286 | 5.604114e-55 | -8.615850e-47 |
| Msa0706230 | Msa0972680 | 0.838232 | 3.250539e-57 | -8.615850e-47 |
| Msa0736920 | Msa0972680 | 0.815049 | 1.127987e-51 | -8.615850e-47 |
| Msa0778570 | Msa0972680 | 0.809337 | 1.988755e-50 | -8.615850e-47 |
| Msa0792370 | Msa0972680 | 0.800317 | 1.525134e-48 | -8.615850e-47 |
| Msa0795750 | Msa0972680 | 0.810114 | 1.353756e-50 | -8.615850e-47 |
| Msa0805020 | Msa0972680 | 0.825995 | 3.457765e-54 | -8.615850e-47 |
| Msa0805050 | Msa0972680 | 0.834446 | 2.983460e-56 | -8.615850e-47 |
| Msa0828950 | Msa0972680 | 0.835544 | 1.577942e-56 | -8.615850e-47 |
| Msa0828960 | Msa0972680 | 0.831789 | 1.367757e-55 | -8.615850e-47 |
| Msa0842330 | Msa0972680 | 0.841290 | 5.196917e-58 | -8.615850e-47 |
| Msa0152850 | Msa0972680 | 0.809419 | 1.909798e-50 | -8.615850e-47 |
| Msa0217060 | Msa0972680 | 0.806569 | 7.717190e-50 | -8.615850e-47 |
| Msa0365210 | Msa0972680 | 0.808010 | 3.820132e-50 | -8.615850e-47 |
| Msa0372300 | Msa0972680 | 0.804604 | 1.994349e-49 | -8.615850e-47 |
| Msa0418450 | Msa0972680 | 0.805885 | 1.075414e-49 | -8.615850e-47 |
| Msa0453040 | Msa0972680 | 0.816313 | 5.897949e-52 | -8.615850e-47 |
| Msa0465500 | Msa0972680 | 0.802953 | 4.392503e-49 | -8.615850e-47 |
| Msa0256920 | Msa0972680 | 0.825418 | 4.738837e-54 | -8.615850e-47 |
| Msa0269970 | Msa0972680 | 0.844335 | 8.058176e-59 | -8.615850e-47 |
| Msa0307670 | Msa0972680 | 0.800756 | 1.241488e-48 | -8.615850e-47 |
| Msa0330520 | Msa0972680 | 0.812530 | 4.048047e-51 | -8.615850e-47 |
| Msa0358300 | Msa0972680 | 0.825993 | 3.461384e-54 | -8.615850e-47 |
| Msa0512630 | Msa0972680 | 0.802672 | 5.020051e-49 | -8.615850e-47 |
| Msa0570120 | Msa0972680 | 0.833373 | 5.537099e-56 | -8.615850e-47 |
| Msa0576790 | Msa0972680 | 0.850873 | 1.281441e-60 | -8.615850e-47 |
| Msa0579220 | Msa0972680 | 0.801066 | 1.072788e-48 | -8.615850e-47 |
| Msa0596840 | Msa0972680 | 0.806838 | 6.772714e-50 | -8.615850e-47 |
| Msa0869990 | Msa0972680 | 0.876083 | 1.850598e-68 | -8.615850e-47 |
| Msa0891590 | Msa0972680 | 0.805576 | 1.248592e-49 | -8.615850e-47 |
| Msa0903310 | Msa0972680 | 0.894236 | 2.981731e-75 | -8.615850e-47 |
| Msa0909260 | Msa0972680 | 0.802352 | 5.844001e-49 | -8.615850e-47 |
| Msa0935720 | Msa0972680 | 0.935031 | 1.605586e-96 | -8.615850e-47 |
| Msa0972680 | Msa0992510 | 0.809253 | 2.073488e-50 | -8.615850e-47 |
| Msa0972680 | Msa1021590 | 0.861974 | 7.034721e-64 | -8.615850e-47 |
| Msa0972680 | Msa1044680 | 0.805035 | 1.621020e-49 | -8.615850e-47 |
| Msa0972680 | Msa1105640 | 0.816451 | 5.493348e-52 | -8.615850e-47 |
| Msa0972680 | Msa1133750 | 0.805568 | 1.253922e-49 | -8.615850e-47 |
| Msa0972680 | Msa1148960 | 0.838177 | 3.357382e-57 | -8.615850e-47 |
| Msa0972680 | Msa1154320 | 0.832243 | 1.056540e-55 | -8.615850e-47 |
| Msa0972680 | Msa1242130 | 0.806438 | 8.226176e-50 | -8.615850e-47 |
| Msa0972680 | Msa1250610 | 0.826377 | 2.805157e-54 | -8.615850e-47 |
| Msa0972680 | Msa1283930 | 0.826163 | 3.153592e-54 | -8.615850e-47 |
| Msa0972680 | Msa1283960 | 0.826009 | 3.432175e-54 | -8.615850e-47 |
| Msa0972680 | Msa1284000 | 0.826630 | 2.441012e-54 | -8.615850e-47 |
| Msa0972680 | Msa1322960 | 0.811856 | 5.677647e-51 | -8.615850e-47 |
| Msa0972680 | Msa1323070 | 0.801047 | 1.082580e-48 | -8.615850e-47 |
| Msa0972680 | Msa1326640 | 0.806335 | 8.646012e-50 | -8.615850e-47 |
| Msa0972680 | Msa1326680 | 0.828323 | 9.581452e-55 | -8.615850e-47 |
| Msa0972680 | Msa1326710 | 0.823408 | 1.407968e-53 | -8.615850e-47 |
| Msa0972680 | Msa1343600 | 0.814026 | 1.899560e-51 | -8.615850e-47 |
| Msa0972680 | Msa1408390 | 0.801225 | 9.952901e-49 | -8.615850e-47 |
| Msa0972680 | Msa1410160 | 0.802393 | 5.732140e-49 | -8.615850e-47 |
| Msa0972680 | Msa1422860 | 0.806987 | 6.298919e-50 | -8.615850e-47 |
| Msa0972680 | Msa1427100 | 0.800628 | 1.318563e-48 | -8.615850e-47 |
| Msa0972680 | Msa1439250 | 0.814257 | 1.689024e-51 | -8.615850e-47 |
| Msa0972680 | Msa1439270 | 0.823887 | 1.087048e-53 | -8.615850e-47 |
| Msa0972680 | Msa1445530 | 0.817929 | 2.555270e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0972680 | MtrunA17_Chr2g0325301 | 93.919 | 148 | 9 | 0 | 1 | 148 | 1 | 148 | 3.08e-101 | 286 |
| Msa0972680 | MtrunA17_Chr6g0451421 | 56.552 | 145 | 61 | 1 | 1 | 145 | 1 | 143 | 4.40e-54 | 167 |
| Msa0972680 | MtrunA17_Chr6g0451411 | 59.559 | 136 | 55 | 0 | 10 | 145 | 176 | 311 | 4.00e-53 | 170 |
| Msa0972680 | MtrunA17_Chr2g0329701 | 50.714 | 140 | 69 | 0 | 9 | 148 | 233 | 372 | 2.86e-46 | 154 |
| Msa0972680 | MtrunA17_Chr7g0264421 | 51.095 | 137 | 67 | 0 | 9 | 145 | 203 | 339 | 7.25e-46 | 152 |
| Msa0972680 | MtrunA17_Chr6g0451441 | 45.695 | 151 | 74 | 1 | 3 | 145 | 313 | 463 | 3.20e-38 | 135 |
| Msa0972680 | MtrunA17_Chr6g0451451 | 52.439 | 82 | 39 | 0 | 64 | 145 | 1 | 82 | 3.03e-26 | 94.7 |
| Msa0972680 | MtrunA17_Chr1g0158081 | 36.975 | 119 | 73 | 1 | 15 | 133 | 1211 | 1327 | 8.94e-17 | 76.3 |
| Msa0972680 | MtrunA17_Chr3g0110591 | 36.134 | 119 | 74 | 1 | 15 | 133 | 27 | 143 | 1.00e-15 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0972680 | AT5G51080.3 | 51.408 | 142 | 69 | 0 | 4 | 145 | 116 | 257 | 9.04e-46 | 150 |
| Msa0972680 | AT3G01410.2 | 49.265 | 136 | 69 | 0 | 10 | 145 | 154 | 289 | 1.44e-45 | 150 |
| Msa0972680 | AT3G01410.1 | 49.265 | 136 | 69 | 0 | 10 | 145 | 154 | 289 | 1.44e-45 | 150 |
| Msa0972680 | AT5G51080.1 | 51.408 | 142 | 69 | 0 | 4 | 145 | 179 | 320 | 4.82e-45 | 150 |
| Msa0972680 | AT5G51080.2 | 51.408 | 142 | 69 | 0 | 4 | 145 | 179 | 320 | 4.82e-45 | 150 |
| Msa0972680 | AT1G24090.1 | 52.174 | 138 | 66 | 0 | 8 | 145 | 214 | 351 | 3.83e-42 | 143 |
Find 62 sgRNAs with CRISPR-Local
Find 2 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCATAGGTTCCTTAAATT+TGG | 0.076392 | 6_4:-4982792 | Msa0972690:intron |
| AGCCTACTAGTTTAGCATTT+AGG | 0.177082 | 6_4:+4989268 | Msa0972680:three_prime_UTR |
| GCCTACTAGTTTAGCATTTA+GGG | 0.220101 | 6_4:+4989269 | Msa0972680:three_prime_UTR |
| TGAGTATAATGGTCTTCTTT+TGG | 0.243282 | 6_4:+4982408 | Msa0972680:CDS |
| CAAAAGTTTAACAAATTTAT+CGG | 0.281331 | 6_4:-4989582 | None:intergenic |
| TCTTGTATTGTTGAGTTTGA+TGG | 0.294112 | 6_4:+4982183 | Msa0972680:CDS |
| CTCTCAGCTTGTTTGCAATC+AGG | 0.296249 | 6_4:+4982483 | Msa0972680:CDS |
| CTATGTTACTTGTCTTGTTT+AGG | 0.312433 | 6_4:+4989242 | Msa0972680:three_prime_UTR |
| TGCAACAGTTTGAGGGTTCC+TGG | 0.323750 | 6_4:+4982757 | Msa0972680:intron |
| TGCATAGGTTCCTTAAATTT+GGG | 0.326607 | 6_4:-4982791 | Msa0972690:intron |
| AAGTCAACAACCCAAATTTA+AGG | 0.331211 | 6_4:+4982781 | Msa0972680:CDS |
| ATTTGGGTTGTTGACTTTCC+AGG | 0.336100 | 6_4:-4982775 | Msa0972690:intron |
| ATAGCTTAAGAGGCTTAAAA+TGG | 0.352852 | 6_4:+4989381 | Msa0972680:three_prime_UTR |
| AATAATTCTGCTGAGTATAA+TGG | 0.382560 | 6_4:+4982397 | Msa0972680:CDS |
| TATGACTTGATGGTATGTTT+TGG | 0.398853 | 6_4:+4989535 | Msa0972680:three_prime_UTR |
| GCTCCTGCACCAGACCTTCC+AGG | 0.402463 | 6_4:-4982222 | None:intergenic |
| ATATTCCACCATAGCTTAAG+AGG | 0.411192 | 6_4:+4989371 | Msa0972680:three_prime_UTR |
| GACTTGATGGTATGACTTGA+TGG | 0.443699 | 6_4:+4989525 | Msa0972680:three_prime_UTR |
| AGAGTAACTTCAAGTCAGTC+AGG | 0.457011 | 6_4:+4982832 | Msa0972680:CDS |
| GGAGCATCCAGTGGAAATCC+TGG | 0.459158 | 6_4:+4982204 | Msa0972680:CDS |
| TCCGTTCTGCAGATGGGAGT+CGG | 0.461814 | 6_4:+4982250 | Msa0972680:CDS |
| CATGGCGTCAGCCAAGGACT+TGG | 0.463996 | 6_4:+4982364 | Msa0972680:CDS |
| CATGAATATGCTCATATCCT+TGG | 0.464886 | 6_4:-4982450 | None:intergenic |
| CCCCTAAATGCTAAACTAGT+AGG | 0.469952 | 6_4:-4989270 | None:intergenic |
| AGTGGAAATCCTGGAAGGTC+TGG | 0.475520 | 6_4:+4982213 | Msa0972680:CDS |
| TATGTCTCTTTATAGGTCCA+TGG | 0.481724 | 6_4:+4982346 | Msa0972680:intron |
| TCAGGGTCGAACATGTTCCT+AGG | 0.482331 | 6_4:+4982850 | Msa0972680:CDS |
| CCATGGCGAACATGACTTGA+TGG | 0.488890 | 6_4:+4989512 | Msa0972680:three_prime_UTR |
| TAATGTTTGAATTTCATGCT+TGG | 0.494753 | 6_4:+4989412 | Msa0972680:three_prime_UTR |
| CCGTTCTGCAGATGGGAGTC+GGG | 0.496764 | 6_4:+4982251 | Msa0972680:CDS |
| TTGTTTGAGTGCCAAGTCCT+TGG | 0.498512 | 6_4:-4982375 | None:intergenic |
| TTAAGCCTCTTAAGCTATGG+TGG | 0.531898 | 6_4:-4989376 | None:intergenic |
| GAACCTATGCAATGAGGCTG+TGG | 0.532829 | 6_4:+4982803 | Msa0972680:CDS |
| GCTCCACAGCCTCATTGCAT+AGG | 0.541509 | 6_4:-4982806 | Msa0972690:intron |
| ATGTGACACTGCATGTCAAA+GGG | 0.541910 | 6_4:+4989302 | Msa0972680:three_prime_UTR |
| CAAGTCCTTGGCTGACGCCA+TGG | 0.543645 | 6_4:-4982363 | None:intergenic |
| CTGTTATCCGTTCTGCAGAT+GGG | 0.545170 | 6_4:+4982244 | Msa0972680:CDS |
| GCTGTTATCCGTTCTGCAGA+TGG | 0.548893 | 6_4:+4982243 | Msa0972680:CDS |
| CATGTGACACTGCATGTCAA+AGG | 0.549075 | 6_4:+4989301 | Msa0972680:three_prime_UTR |
| AGACCTTCCAGGATTTCCAC+TGG | 0.556723 | 6_4:-4982211 | None:intergenic |
| TAGGTCCATGGCGTCAGCCA+AGG | 0.557191 | 6_4:+4982358 | Msa0972680:intron |
| AATCCTGGAAGGTCTGGTGC+AGG | 0.565510 | 6_4:+4982219 | Msa0972680:CDS |
| GTAGTAATATTCAACTTCGC+CGG | 0.574495 | 6_4:-4989179 | Msa0972690:five_prime_UTR |
| CCTACTAGTTTAGCATTTAG+GGG | 0.580750 | 6_4:+4989270 | Msa0972680:three_prime_UTR |
| TAATATCTTGTGCTATGCCA+TGG | 0.581364 | 6_4:+4989495 | Msa0972680:three_prime_UTR |
| CTACTAGTTTAGCATTTAGG+GGG | 0.582134 | 6_4:+4989271 | Msa0972680:three_prime_UTR |
| ATGGTGGAATATACACATCA+TGG | 0.582584 | 6_4:-4989360 | None:intergenic |
| CTGAAAGAAGCTAGCAACCA+AGG | 0.584237 | 6_4:+4982433 | Msa0972680:CDS |
| CAGGGTCGAACATGTTCCTA+GGG | 0.587867 | 6_4:+4982851 | Msa0972680:CDS |
| CCATCAAGTCATGTTCGCCA+TGG | 0.612238 | 6_4:-4989512 | None:intergenic |
| GAGCAAACTGAGAGAACCGT+AGG | 0.613306 | 6_4:+4989604 | Msa0972680:three_prime_UTR |
| TATGGGTGGTGATTGACCTA+CGG | 0.619063 | 6_4:-4989620 | None:intergenic |
| CCCGACTCCCATCTGCAGAA+CGG | 0.635424 | 6_4:-4982251 | None:intergenic |
| TTTAAGGAACCTATGCAATG+AGG | 0.642695 | 6_4:+4982797 | Msa0972680:CDS |
| GAGTTTGATGGAGCATCCAG+TGG | 0.644852 | 6_4:+4982195 | Msa0972680:CDS |
| GAGTAACTTCAAGTCAGTCA+GGG | 0.650851 | 6_4:+4982833 | Msa0972680:CDS |
| CATCCAGTGGAAATCCTGGA+AGG | 0.657104 | 6_4:+4982208 | Msa0972680:CDS |
| TATGAGCATATTCATGTCCG+AGG | 0.670788 | 6_4:+4982457 | Msa0972680:CDS |
| GCTGATTCTCAAGCAAATCG+CGG | 0.685342 | 6_4:+4988907 | Msa0972680:CDS |
| GAAATTCATAAACATTCACA+TGG | 0.691845 | 6_4:-4989438 | None:intergenic |
| AAACAAGCTGAGAGTCACCT+CGG | 0.700596 | 6_4:-4982474 | None:intergenic |
| GAGTCGTGGAAACAACAGAG+AGG | 0.767113 | 6_4:+4980102 | Msa0972680:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCTACTCTTTTTCTGTTTA+TGG | - | chr6_4:4980075-4980094 | None:intergenic | 25.0% |
| AGTAGAAAAATCATGAGTCG+TGG | + | chr6_4:4980088-4980107 | Msa0972680:exon | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 4980051 | 4989636 | 4980051 | ID=Msa0972680;Name=Msa0972680 |
| chr6_4 | mRNA | 4980051 | 4989636 | 4980051 | ID=Msa0972680-mRNA-1;Parent=Msa0972680;Name=Msa0972680-mRNA-1;_AED=0.36;_eAED=0.36;_QI=49|1|1|1|0.8|1|6|432|148 |
| chr6_4 | exon | 4980051 | 4980123 | 4980051 | ID=Msa0972680-mRNA-1:exon:2184;Parent=Msa0972680-mRNA-1 |
| chr6_4 | exon | 4982180 | 4982272 | 4982180 | ID=Msa0972680-mRNA-1:exon:2185;Parent=Msa0972680-mRNA-1 |
| chr6_4 | exon | 4982361 | 4982504 | 4982361 | ID=Msa0972680-mRNA-1:exon:2186;Parent=Msa0972680-mRNA-1 |
| chr6_4 | exon | 4982765 | 4982872 | 4982765 | ID=Msa0972680-mRNA-1:exon:2187;Parent=Msa0972680-mRNA-1 |
| chr6_4 | exon | 4988892 | 4988943 | 4988892 | ID=Msa0972680-mRNA-1:exon:2188;Parent=Msa0972680-mRNA-1 |
| chr6_4 | exon | 4989179 | 4989636 | 4989179 | ID=Msa0972680-mRNA-1:exon:2189;Parent=Msa0972680-mRNA-1 |
| chr6_4 | five_prime_UTR | 4980051 | 4980099 | 4980051 | ID=Msa0972680-mRNA-1:five_prime_utr;Parent=Msa0972680-mRNA-1 |
| chr6_4 | CDS | 4980100 | 4980123 | 4980100 | ID=Msa0972680-mRNA-1:cds;Parent=Msa0972680-mRNA-1 |
| chr6_4 | CDS | 4982180 | 4982272 | 4982180 | ID=Msa0972680-mRNA-1:cds;Parent=Msa0972680-mRNA-1 |
| chr6_4 | CDS | 4982361 | 4982504 | 4982361 | ID=Msa0972680-mRNA-1:cds;Parent=Msa0972680-mRNA-1 |
| chr6_4 | CDS | 4982765 | 4982872 | 4982765 | ID=Msa0972680-mRNA-1:cds;Parent=Msa0972680-mRNA-1 |
| chr6_4 | CDS | 4988892 | 4988943 | 4988892 | ID=Msa0972680-mRNA-1:cds;Parent=Msa0972680-mRNA-1 |
| chr6_4 | CDS | 4989179 | 4989204 | 4989179 | ID=Msa0972680-mRNA-1:cds;Parent=Msa0972680-mRNA-1 |
| chr6_4 | three_prime_UTR | 4989205 | 4989636 | 4989205 | ID=Msa0972680-mRNA-1:three_prime_utr;Parent=Msa0972680-mRNA-1 |
| Gene Sequence |
| Protein sequence |
