AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0957100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0957100 MtrunA17_Chr3g0115561 88.393 112 13 0 19 130 51 162 1.86e-70 208
Msa0957100 MtrunA17_Chr3g0124301 34.211 114 73 2 14 125 43 156 8.15e-20 79.7
Msa0957100 MtrunA17_Chr2g0283801 36.111 108 65 2 19 123 51 157 1.38e-16 71.6
Msa0957100 MtrunA17_Chr2g0316111 34.188 117 71 3 7 123 57 167 1.58e-13 63.9
Msa0957100 MtrunA17_Chr2g0316151 34.188 117 71 3 7 123 57 167 1.96e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0957100 AT4G08685.1 44.660 103 57 0 19 121 50 152 3.06e-29 104
Msa0957100 AT1G78040.1 34.400 125 81 1 7 130 42 166 4.20e-20 81.3
Msa0957100 AT1G78040.3 34.400 125 81 1 7 130 42 166 4.20e-20 81.3
Msa0957100 AT1G78040.2 34.400 125 81 1 7 130 42 166 4.20e-20 81.3
Msa0957100 AT5G10130.1 38.462 104 64 0 21 124 53 156 1.32e-16 72.0
Msa0957100 AT4G18596.1 33.929 112 70 2 16 123 53 164 1.32e-15 69.7
Msa0957100 AT5G45880.1 33.036 112 71 2 16 123 55 166 2.32e-15 68.9
Msa0957100 AT4G18596.2 33.929 112 70 2 16 123 99 210 2.56e-15 69.7
Msa0957100 AT1G29140.1 34.821 112 69 2 16 123 52 163 3.74e-14 65.9

Find 41 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATGCTTATATATGTTAT+TGG 0.218578 6_3:+66900473 Msa0957100:three_prime_UTR
TAGTTTATTTACTTTGGATT+TGG 0.238080 6_3:+66900387 Msa0957100:three_prime_UTR
AGTTTATTTACTTTGGATTT+GGG 0.259659 6_3:+66900388 Msa0957100:three_prime_UTR
AATTCCTAGTTTATTTACTT+TGG 0.368713 6_3:+66900381 Msa0957100:three_prime_UTR
CCAGTTGCATCAGTCTTCCC+TGG 0.381486 6_3:-66900102 None:intergenic
CCTCAAGCAAATGCTGGAAT+TGG 0.390695 6_3:+66900338 Msa0957100:CDS
CTTAATTATATTGTTTATTG+AGG 0.395561 6_3:+66900504 Msa0957100:three_prime_UTR
ATTTGATTCAATCTTGATGA+TGG 0.396339 6_3:+66900424 Msa0957100:three_prime_UTR
ATGGCAGAGGAGGTTGCTTC+TGG 0.397308 6_3:+66900303 Msa0957100:CDS
TGCTGACCAATTTGAAGAAA+AGG 0.419907 6_3:+66898518 Msa0957100:CDS
AAACAATATAATTAAGCATA+TGG 0.421222 6_3:-66900497 None:intergenic
GATTGAATCAAATAGACAAT+AGG 0.435433 6_3:-66900414 None:intergenic
GGAGGCAATGCCGTTGTAAC+GGG 0.439821 6_3:-66900250 None:intergenic
AGTTGTAGCGAAAGAGCACC+TGG 0.442879 6_3:+66900201 Msa0957100:CDS
CCAGGGAAGACTGATGCAAC+TGG 0.458783 6_3:+66900102 Msa0957100:CDS
TTTAGGTGCCGAGGTTATGC+TGG 0.462938 6_3:+66900038 Msa0957100:intron
AGCATTGGCAAACCTGTCAT+TGG 0.466304 6_3:-66900271 None:intergenic
TGTTTGCATACAATGGTTGA+TGG 0.506858 6_3:+66898495 None:intergenic
TGGAGGCAATGCCGTTGTAA+CGG 0.522858 6_3:-66900251 None:intergenic
TTATTGAGGATCATGTGTCT+TGG 0.527504 6_3:+66900518 Msa0957100:three_prime_UTR
GGGTGAGAATAACGCGTGCT+TGG 0.527810 6_3:-66900230 None:intergenic
CCAATTCCAGCATTTGCTTG+AGG 0.528978 6_3:-66900338 None:intergenic
ACGGCATTGCCTCCAATGAC+AGG 0.539090 6_3:+66900259 Msa0957100:CDS
TAGTCTCAGTTCTGTTGCTG+TGG 0.539457 6_3:+66900551 Msa0957100:three_prime_UTR
TGCTATGTTGTACATGGCAG+AGG 0.540527 6_3:+66900290 Msa0957100:CDS
TGCCAATGCTATGTTGTACA+TGG 0.550672 6_3:+66900284 Msa0957100:CDS
ACGCGTGCTTGGTCACGCCC+AGG 0.556503 6_3:-66900219 None:intergenic
TGAGGTCCTCAAGCAAATGC+TGG 0.562685 6_3:+66900332 Msa0957100:CDS
ATTGGCAAACCTGTCATTGG+AGG 0.565263 6_3:-66900268 None:intergenic
AAATCCAAAGTAAATAAACT+AGG 0.569739 6_3:-66900385 None:intergenic
CTTGCATTCCAGCATAACCT+CGG 0.573523 6_3:-66900046 None:intergenic
TGAAGAAAAGGATCCAAATG+AGG 0.582440 6_3:+66898530 Msa0957100:CDS
TGCCATGTACAACATAGCAT+TGG 0.582518 6_3:-66900286 None:intergenic
GTTATTCTCACCCGTTACAA+CGG 0.584831 6_3:+66900240 Msa0957100:CDS
GGTTGCTTCTGGCTGTGCTG+AGG 0.590498 6_3:+66900314 Msa0957100:CDS
TGGAGAATATACAATCTATG+TGG 0.605358 6_3:+66900122 Msa0957100:CDS
ATGAATCTGTTTAGGTGCCG+AGG 0.607790 6_3:+66900029 Msa0957100:intron
CTTTCGCTACAACTACGCTC+AGG 0.622028 6_3:-66900192 None:intergenic
TTATGCTGGAATGCAAGCAA+AGG 0.653268 6_3:+66900052 Msa0957100:CDS
TATGTTGTACATGGCAGAGG+AGG 0.691786 6_3:+66900293 Msa0957100:CDS
GTTGTAGCGAAAGAGCACCT+GGG 0.714138 6_3:+66900202 Msa0957100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTATAAATTACAAAAATTT+AGG - chr6_3:66899107-66899126 None:intergenic 10.0%
!! TGTTAAAAAAATAACATTTA+TGG + chr6_3:66899199-66899218 Msa0957100:intron 10.0%
!!! TTTTGTATTATCAATTAAAT+TGG + chr6_3:66899575-66899594 Msa0957100:intron 10.0%
!! CTTATAAATTACAAAAATTT+AGG - chr6_3:66899107-66899126 None:intergenic 10.0%
!! TGTTAAAAAAATAACATTTA+TGG + chr6_3:66899199-66899218 Msa0957100:intron 10.0%
!!! TTTTGTATTATCAATTAAAT+TGG + chr6_3:66899575-66899594 Msa0957100:intron 10.0%
!! AGTTGAAATTAATTAATGTA+TGG - chr6_3:66898912-66898931 None:intergenic 15.0%
!! TTAGTAAATGAATGTTAATA+AGG + chr6_3:66898966-66898985 Msa0957100:intron 15.0%
!!! ATTGATAATACAAAATGTAT+AGG - chr6_3:66899570-66899589 None:intergenic 15.0%
!! TATATGCTTATATATGTTAT+TGG + chr6_3:66900473-66900492 Msa0957100:three_prime_UTR 15.0%
!! AAACAATATAATTAAGCATA+TGG - chr6_3:66900500-66900519 None:intergenic 15.0%
!! CTTAATTATATTGTTTATTG+AGG + chr6_3:66900504-66900523 Msa0957100:three_prime_UTR 15.0%
!! AGTTGAAATTAATTAATGTA+TGG - chr6_3:66898912-66898931 None:intergenic 15.0%
!! TTAGTAAATGAATGTTAATA+AGG + chr6_3:66898966-66898985 Msa0957100:intron 15.0%
!!! ATTGATAATACAAAATGTAT+AGG - chr6_3:66899570-66899589 None:intergenic 15.0%
!! TATATGCTTATATATGTTAT+TGG + chr6_3:66900473-66900492 Msa0957100:three_prime_UTR 15.0%
!! AAACAATATAATTAAGCATA+TGG - chr6_3:66900500-66900519 None:intergenic 15.0%
!! CTTAATTATATTGTTTATTG+AGG + chr6_3:66900504-66900523 Msa0957100:three_prime_UTR 15.0%
!! AAAGACTAAACAATATTACT+TGG - chr6_3:66898848-66898867 None:intergenic 20.0%
!! AAGAAATTAAACTGAGAAAT+TGG - chr6_3:66899371-66899390 None:intergenic 20.0%
!! TGTATATTATATCCCAATAT+TGG - chr6_3:66899495-66899514 None:intergenic 20.0%
!!! CTAAATTTTTTGTTTTGTCA+TGG + chr6_3:66899732-66899751 Msa0957100:intron 20.0%
!! TTTGATTAATGAATCTGTTT+AGG + chr6_3:66900021-66900040 Msa0957100:intron 20.0%
!! AATTCCTAGTTTATTTACTT+TGG + chr6_3:66900381-66900400 Msa0957100:three_prime_UTR 20.0%
!! AAATCCAAAGTAAATAAACT+AGG - chr6_3:66900388-66900407 None:intergenic 20.0%
!! TAGTTTATTTACTTTGGATT+TGG + chr6_3:66900387-66900406 Msa0957100:three_prime_UTR 20.0%
!! AGTTTATTTACTTTGGATTT+GGG + chr6_3:66900388-66900407 Msa0957100:three_prime_UTR 20.0%
!! AAAGACTAAACAATATTACT+TGG - chr6_3:66898848-66898867 None:intergenic 20.0%
!! AAGAAATTAAACTGAGAAAT+TGG - chr6_3:66899371-66899390 None:intergenic 20.0%
!! TGTATATTATATCCCAATAT+TGG - chr6_3:66899495-66899514 None:intergenic 20.0%
!!! CTAAATTTTTTGTTTTGTCA+TGG + chr6_3:66899732-66899751 Msa0957100:intron 20.0%
!! TTTGATTAATGAATCTGTTT+AGG + chr6_3:66900021-66900040 Msa0957100:intron 20.0%
!! AATTCCTAGTTTATTTACTT+TGG + chr6_3:66900381-66900400 Msa0957100:three_prime_UTR 20.0%
!! AAATCCAAAGTAAATAAACT+AGG - chr6_3:66900388-66900407 None:intergenic 20.0%
!! TAGTTTATTTACTTTGGATT+TGG + chr6_3:66900387-66900406 Msa0957100:three_prime_UTR 20.0%
!! AGTTTATTTACTTTGGATTT+GGG + chr6_3:66900388-66900407 Msa0957100:three_prime_UTR 20.0%
! ATAACTATAGCTTCTTCTAT+TGG + chr6_3:66898675-66898694 Msa0957100:intron 25.0%
!! TGTATGGTACTTTTACAATA+GGG - chr6_3:66898896-66898915 None:intergenic 25.0%
!! ATGTATGGTACTTTTACAAT+AGG - chr6_3:66898897-66898916 None:intergenic 25.0%
! TATATCACACACTTACATTA+GGG + chr6_3:66899017-66899036 Msa0957100:intron 25.0%
! AAAATAACTTAATTTCCACC+CGG - chr6_3:66899291-66899310 None:intergenic 25.0%
!!! TACTATTGACTTTCCAATAT+TGG + chr6_3:66899479-66899498 Msa0957100:intron 25.0%
!!! ACTATTGACTTTCCAATATT+GGG + chr6_3:66899480-66899499 Msa0957100:intron 25.0%
!! TAACAAACAGCAATTTTCTT+TGG - chr6_3:66899988-66900007 None:intergenic 25.0%
! GATTGAATCAAATAGACAAT+AGG - chr6_3:66900417-66900436 None:intergenic 25.0%
! ATTTGATTCAATCTTGATGA+TGG + chr6_3:66900424-66900443 Msa0957100:three_prime_UTR 25.0%
! ATAACTATAGCTTCTTCTAT+TGG + chr6_3:66898675-66898694 Msa0957100:intron 25.0%
!! TGTATGGTACTTTTACAATA+GGG - chr6_3:66898896-66898915 None:intergenic 25.0%
!! ATGTATGGTACTTTTACAAT+AGG - chr6_3:66898897-66898916 None:intergenic 25.0%
! TATATCACACACTTACATTA+GGG + chr6_3:66899017-66899036 Msa0957100:intron 25.0%
! AAAATAACTTAATTTCCACC+CGG - chr6_3:66899291-66899310 None:intergenic 25.0%
!!! TACTATTGACTTTCCAATAT+TGG + chr6_3:66899479-66899498 Msa0957100:intron 25.0%
!!! ACTATTGACTTTCCAATATT+GGG + chr6_3:66899480-66899499 Msa0957100:intron 25.0%
!! TAACAAACAGCAATTTTCTT+TGG - chr6_3:66899988-66900007 None:intergenic 25.0%
! GATTGAATCAAATAGACAAT+AGG - chr6_3:66900417-66900436 None:intergenic 25.0%
! ATTTGATTCAATCTTGATGA+TGG + chr6_3:66900424-66900443 Msa0957100:three_prime_UTR 25.0%
! TTGGATCCTTTTCTTCAAAT+TGG - chr6_3:66898527-66898546 None:intergenic 30.0%
!!! CACTTGCAAGTTTTTTCAAT+TGG + chr6_3:66898559-66898578 Msa0957100:intron 30.0%
!!! GGAGTTTTGATTCTATAGAA+AGG - chr6_3:66898726-66898745 None:intergenic 30.0%
AGTCTTTGTACATACTTTAG+TGG + chr6_3:66898861-66898880 Msa0957100:intron 30.0%
TAATAAGGTCAATATCTGCT+AGG + chr6_3:66898981-66899000 Msa0957100:intron 30.0%
GTATATCACACACTTACATT+AGG + chr6_3:66899016-66899035 Msa0957100:intron 30.0%
!!! TGCATGCTTTTTTAATCTCT+AGG + chr6_3:66899077-66899096 Msa0957100:intron 30.0%
TGATCAATATTTATACACGC+AGG + chr6_3:66899542-66899561 Msa0957100:intron 30.0%
GATGTGTGTTTACATGAAAA+TGG + chr6_3:66899703-66899722 Msa0957100:intron 30.0%
ATCCAAACAAGACTTAAGTT+TGG + chr6_3:66899828-66899847 Msa0957100:intron 30.0%
!! AACCAAACTTAAGTCTTGTT+TGG - chr6_3:66899833-66899852 None:intergenic 30.0%
!! AACATCATCTCTAGAGTATA+TGG + chr6_3:66899889-66899908 Msa0957100:intron 30.0%
GTTAAAAATCCAGCCATAAA+AGG - chr6_3:66899954-66899973 None:intergenic 30.0%
! TTTATGGCTGGATTTTTAAC+AGG + chr6_3:66899954-66899973 Msa0957100:intron 30.0%
!! AAGTTGTTTTCTCAAAACCA+GGG + chr6_3:66900085-66900104 Msa0957100:CDS 30.0%
TGGAGAATATACAATCTATG+TGG + chr6_3:66900122-66900141 Msa0957100:CDS 30.0%
! TTGGATCCTTTTCTTCAAAT+TGG - chr6_3:66898527-66898546 None:intergenic 30.0%
!!! CACTTGCAAGTTTTTTCAAT+TGG + chr6_3:66898559-66898578 Msa0957100:intron 30.0%
!!! GGAGTTTTGATTCTATAGAA+AGG - chr6_3:66898726-66898745 None:intergenic 30.0%
AGTCTTTGTACATACTTTAG+TGG + chr6_3:66898861-66898880 Msa0957100:intron 30.0%
TAATAAGGTCAATATCTGCT+AGG + chr6_3:66898981-66899000 Msa0957100:intron 30.0%
GTATATCACACACTTACATT+AGG + chr6_3:66899016-66899035 Msa0957100:intron 30.0%
!!! TGCATGCTTTTTTAATCTCT+AGG + chr6_3:66899077-66899096 Msa0957100:intron 30.0%
TGATCAATATTTATACACGC+AGG + chr6_3:66899542-66899561 Msa0957100:intron 30.0%
GATGTGTGTTTACATGAAAA+TGG + chr6_3:66899703-66899722 Msa0957100:intron 30.0%
ATCCAAACAAGACTTAAGTT+TGG + chr6_3:66899828-66899847 Msa0957100:intron 30.0%
!! AACCAAACTTAAGTCTTGTT+TGG - chr6_3:66899833-66899852 None:intergenic 30.0%
!! AACATCATCTCTAGAGTATA+TGG + chr6_3:66899889-66899908 Msa0957100:intron 30.0%
GTTAAAAATCCAGCCATAAA+AGG - chr6_3:66899954-66899973 None:intergenic 30.0%
! TTTATGGCTGGATTTTTAAC+AGG + chr6_3:66899954-66899973 Msa0957100:intron 30.0%
!! AAGTTGTTTTCTCAAAACCA+GGG + chr6_3:66900085-66900104 Msa0957100:CDS 30.0%
TGGAGAATATACAATCTATG+TGG + chr6_3:66900122-66900141 Msa0957100:CDS 30.0%
TGCTGACCAATTTGAAGAAA+AGG + chr6_3:66898518-66898537 Msa0957100:CDS 35.0%
TGAAGAAAAGGATCCAAATG+AGG + chr6_3:66898530-66898549 Msa0957100:CDS 35.0%
! CTTTTGCTTAGTTCAACACT+TGG + chr6_3:66898582-66898601 Msa0957100:intron 35.0%
!! TGTACATACTTTAGTGGACT+TGG + chr6_3:66898867-66898886 Msa0957100:intron 35.0%
AAGCATGCAGTAACATAGTT+TGG - chr6_3:66899066-66899085 None:intergenic 35.0%
! AGATTCATGTACCACTCTTT+TGG + chr6_3:66899226-66899245 Msa0957100:intron 35.0%
AGACGATTTCGAGAAATAGA+TGG + chr6_3:66899611-66899630 Msa0957100:intron 35.0%
!! TGGACTTATGTCTGAGATTT+TGG + chr6_3:66899631-66899650 Msa0957100:intron 35.0%
! GCAGTACTGAAGTTAAGAAT+GGG + chr6_3:66899653-66899672 Msa0957100:intron 35.0%
GATGATGTTGTTGTAACTTG+TGG - chr6_3:66899878-66899897 None:intergenic 35.0%
!! TCATCTCTAGAGTATATGGT+TGG + chr6_3:66899893-66899912 Msa0957100:intron 35.0%
! GGTGTTGTGATTCATCTATT+TGG + chr6_3:66899914-66899933 Msa0957100:intron 35.0%
!! GAAGTTGTTTTCTCAAAACC+AGG + chr6_3:66900084-66900103 Msa0957100:CDS 35.0%
TTATTGAGGATCATGTGTCT+TGG + chr6_3:66900518-66900537 Msa0957100:three_prime_UTR 35.0%
TGCTGACCAATTTGAAGAAA+AGG + chr6_3:66898518-66898537 Msa0957100:CDS 35.0%
TGAAGAAAAGGATCCAAATG+AGG + chr6_3:66898530-66898549 Msa0957100:CDS 35.0%
! CTTTTGCTTAGTTCAACACT+TGG + chr6_3:66898582-66898601 Msa0957100:intron 35.0%
!! TGTACATACTTTAGTGGACT+TGG + chr6_3:66898867-66898886 Msa0957100:intron 35.0%
AAGCATGCAGTAACATAGTT+TGG - chr6_3:66899066-66899085 None:intergenic 35.0%
! AGATTCATGTACCACTCTTT+TGG + chr6_3:66899226-66899245 Msa0957100:intron 35.0%
AGACGATTTCGAGAAATAGA+TGG + chr6_3:66899611-66899630 Msa0957100:intron 35.0%
!! TGGACTTATGTCTGAGATTT+TGG + chr6_3:66899631-66899650 Msa0957100:intron 35.0%
! GCAGTACTGAAGTTAAGAAT+GGG + chr6_3:66899653-66899672 Msa0957100:intron 35.0%
GATGATGTTGTTGTAACTTG+TGG - chr6_3:66899878-66899897 None:intergenic 35.0%
!! TCATCTCTAGAGTATATGGT+TGG + chr6_3:66899893-66899912 Msa0957100:intron 35.0%
! GGTGTTGTGATTCATCTATT+TGG + chr6_3:66899914-66899933 Msa0957100:intron 35.0%
!! GAAGTTGTTTTCTCAAAACC+AGG + chr6_3:66900084-66900103 Msa0957100:CDS 35.0%
TTATTGAGGATCATGTGTCT+TGG + chr6_3:66900518-66900537 Msa0957100:three_prime_UTR 35.0%
TCAACACTTGGAGCAATTAC+AGG + chr6_3:66898594-66898613 Msa0957100:intron 40.0%
!! AGGTGTTACTGGTGTTGTTT+GGG + chr6_3:66898614-66898633 Msa0957100:intron 40.0%
ACCTGAGCAATTCTGATTGT+GGG - chr6_3:66898759-66898778 None:intergenic 40.0%
! AAAAGGGTGTACCAAAAGAG+TGG - chr6_3:66899240-66899259 None:intergenic 40.0%
! ACTCTTTTGGTACACCCTTT+TGG + chr6_3:66899239-66899258 Msa0957100:intron 40.0%
ATGAAAGTGAGAGAGAGAGA+GGG - chr6_3:66899353-66899372 None:intergenic 40.0%
AATGAAAGTGAGAGAGAGAG+AGG - chr6_3:66899354-66899373 None:intergenic 40.0%
! GGCAGTACTGAAGTTAAGAA+TGG + chr6_3:66899652-66899671 Msa0957100:intron 40.0%
! TTGTTGTAACTTGTGGAGTG+TGG - chr6_3:66899871-66899890 None:intergenic 40.0%
TTATGCTGGAATGCAAGCAA+AGG + chr6_3:66900052-66900071 Msa0957100:CDS 40.0%
GTTATTCTCACCCGTTACAA+CGG + chr6_3:66900240-66900259 Msa0957100:CDS 40.0%
TGCCAATGCTATGTTGTACA+TGG + chr6_3:66900284-66900303 Msa0957100:CDS 40.0%
TGCCATGTACAACATAGCAT+TGG - chr6_3:66900289-66900308 None:intergenic 40.0%
TCAACACTTGGAGCAATTAC+AGG + chr6_3:66898594-66898613 Msa0957100:intron 40.0%
!! AGGTGTTACTGGTGTTGTTT+GGG + chr6_3:66898614-66898633 Msa0957100:intron 40.0%
ACCTGAGCAATTCTGATTGT+GGG - chr6_3:66898759-66898778 None:intergenic 40.0%
! AAAAGGGTGTACCAAAAGAG+TGG - chr6_3:66899240-66899259 None:intergenic 40.0%
! ACTCTTTTGGTACACCCTTT+TGG + chr6_3:66899239-66899258 Msa0957100:intron 40.0%
ATGAAAGTGAGAGAGAGAGA+GGG - chr6_3:66899353-66899372 None:intergenic 40.0%
AATGAAAGTGAGAGAGAGAG+AGG - chr6_3:66899354-66899373 None:intergenic 40.0%
! GGCAGTACTGAAGTTAAGAA+TGG + chr6_3:66899652-66899671 Msa0957100:intron 40.0%
! TTGTTGTAACTTGTGGAGTG+TGG - chr6_3:66899871-66899890 None:intergenic 40.0%
TTATGCTGGAATGCAAGCAA+AGG + chr6_3:66900052-66900071 Msa0957100:CDS 40.0%
GTTATTCTCACCCGTTACAA+CGG + chr6_3:66900240-66900259 Msa0957100:CDS 40.0%
TGCCAATGCTATGTTGTACA+TGG + chr6_3:66900284-66900303 Msa0957100:CDS 40.0%
TGCCATGTACAACATAGCAT+TGG - chr6_3:66900289-66900308 None:intergenic 40.0%
! GCAAGTGCTTTTGCCTCATT+TGG - chr6_3:66898546-66898565 None:intergenic 45.0%
! GGAGCAATTACAGGTGTTAC+TGG + chr6_3:66898603-66898622 Msa0957100:intron 45.0%
!! CAGGTGTTACTGGTGTTGTT+TGG + chr6_3:66898613-66898632 Msa0957100:intron 45.0%
TACGCAAAGAGCTTGGCATT+GGG + chr6_3:66898641-66898660 Msa0957100:intron 45.0%
CCTGAGCAATTCTGATTGTG+GGG - chr6_3:66898758-66898777 None:intergenic 45.0%
GACCTGAGCAATTCTGATTG+TGG - chr6_3:66898760-66898779 None:intergenic 45.0%
ACTTAATTTCCACCCGGCAA+AGG - chr6_3:66899285-66899304 None:intergenic 45.0%
ATTGGAACTGAGACACAGAG+AGG - chr6_3:66899396-66899415 None:intergenic 45.0%
TTGTGGAGTGTGGACAATAG+TGG - chr6_3:66899861-66899880 None:intergenic 45.0%
TCTATTTGGCCAGAGTACGA+TGG + chr6_3:66899928-66899947 Msa0957100:intron 45.0%
CATAAAAGGCCATCGTACTC+TGG - chr6_3:66899940-66899959 None:intergenic 45.0%
! CAGAGTACGATGGCCTTTTA+TGG + chr6_3:66899938-66899957 Msa0957100:intron 45.0%
! ATGAATCTGTTTAGGTGCCG+AGG + chr6_3:66900029-66900048 Msa0957100:intron 45.0%
CTTGCATTCCAGCATAACCT+CGG - chr6_3:66900049-66900068 None:intergenic 45.0%
ATTGGCAAACCTGTCATTGG+AGG - chr6_3:66900271-66900290 None:intergenic 45.0%
!! AGCATTGGCAAACCTGTCAT+TGG - chr6_3:66900274-66900293 None:intergenic 45.0%
TGCTATGTTGTACATGGCAG+AGG + chr6_3:66900290-66900309 Msa0957100:CDS 45.0%
TATGTTGTACATGGCAGAGG+AGG + chr6_3:66900293-66900312 Msa0957100:CDS 45.0%
CCAATTCCAGCATTTGCTTG+AGG - chr6_3:66900341-66900360 None:intergenic 45.0%
CCTCAAGCAAATGCTGGAAT+TGG + chr6_3:66900338-66900357 Msa0957100:CDS 45.0%
TAGTCTCAGTTCTGTTGCTG+TGG + chr6_3:66900551-66900570 Msa0957100:three_prime_UTR 45.0%
! GCAAGTGCTTTTGCCTCATT+TGG - chr6_3:66898546-66898565 None:intergenic 45.0%
! GGAGCAATTACAGGTGTTAC+TGG + chr6_3:66898603-66898622 Msa0957100:intron 45.0%
!! CAGGTGTTACTGGTGTTGTT+TGG + chr6_3:66898613-66898632 Msa0957100:intron 45.0%
TACGCAAAGAGCTTGGCATT+GGG + chr6_3:66898641-66898660 Msa0957100:intron 45.0%
CCTGAGCAATTCTGATTGTG+GGG - chr6_3:66898758-66898777 None:intergenic 45.0%
GACCTGAGCAATTCTGATTG+TGG - chr6_3:66898760-66898779 None:intergenic 45.0%
ACTTAATTTCCACCCGGCAA+AGG - chr6_3:66899285-66899304 None:intergenic 45.0%
ATTGGAACTGAGACACAGAG+AGG - chr6_3:66899396-66899415 None:intergenic 45.0%
TTGTGGAGTGTGGACAATAG+TGG - chr6_3:66899861-66899880 None:intergenic 45.0%
TCTATTTGGCCAGAGTACGA+TGG + chr6_3:66899928-66899947 Msa0957100:intron 45.0%
CATAAAAGGCCATCGTACTC+TGG - chr6_3:66899940-66899959 None:intergenic 45.0%
! CAGAGTACGATGGCCTTTTA+TGG + chr6_3:66899938-66899957 Msa0957100:intron 45.0%
! ATGAATCTGTTTAGGTGCCG+AGG + chr6_3:66900029-66900048 Msa0957100:intron 45.0%
CTTGCATTCCAGCATAACCT+CGG - chr6_3:66900049-66900068 None:intergenic 45.0%
ATTGGCAAACCTGTCATTGG+AGG - chr6_3:66900271-66900290 None:intergenic 45.0%
!! AGCATTGGCAAACCTGTCAT+TGG - chr6_3:66900274-66900293 None:intergenic 45.0%
TGCTATGTTGTACATGGCAG+AGG + chr6_3:66900290-66900309 Msa0957100:CDS 45.0%
TATGTTGTACATGGCAGAGG+AGG + chr6_3:66900293-66900312 Msa0957100:CDS 45.0%
CCAATTCCAGCATTTGCTTG+AGG - chr6_3:66900341-66900360 None:intergenic 45.0%
CCTCAAGCAAATGCTGGAAT+TGG + chr6_3:66900338-66900357 Msa0957100:CDS 45.0%
TAGTCTCAGTTCTGTTGCTG+TGG + chr6_3:66900551-66900570 Msa0957100:three_prime_UTR 45.0%
! GGGTTAGTACGCAAAGAGCT+TGG + chr6_3:66898634-66898653 Msa0957100:intron 50.0%
GTACGCAAAGAGCTTGGCAT+TGG + chr6_3:66898640-66898659 Msa0957100:intron 50.0%
ACGCAAAGAGCTTGGCATTG+GGG + chr6_3:66898642-66898661 Msa0957100:intron 50.0%
! GGCTTTGTCACACTTGCTCT+TGG + chr6_3:66898696-66898715 Msa0957100:intron 50.0%
CCCCACAATCAGAATTGCTC+AGG + chr6_3:66898755-66898774 Msa0957100:intron 50.0%
CATCAGCGGGTGTACCAAAA+GGG - chr6_3:66899256-66899275 None:intergenic 50.0%
ACATCAGCGGGTGTACCAAA+AGG - chr6_3:66899257-66899276 None:intergenic 50.0%
! TTTTGGTACACCCGCTGATG+TGG + chr6_3:66899256-66899275 Msa0957100:intron 50.0%
! AGAGAGAGAGAGGGTGTGTA+AGG - chr6_3:66899344-66899363 None:intergenic 50.0%
! GTACGATGGCCTTTTATGGC+TGG + chr6_3:66899942-66899961 Msa0957100:intron 50.0%
!! TTTAGGTGCCGAGGTTATGC+TGG + chr6_3:66900038-66900057 Msa0957100:intron 50.0%
CTTTCGCTACAACTACGCTC+AGG - chr6_3:66900195-66900214 None:intergenic 50.0%
!! AGTTGTAGCGAAAGAGCACC+TGG + chr6_3:66900201-66900220 Msa0957100:CDS 50.0%
!! GTTGTAGCGAAAGAGCACCT+GGG + chr6_3:66900202-66900221 Msa0957100:CDS 50.0%
TGGAGGCAATGCCGTTGTAA+CGG - chr6_3:66900254-66900273 None:intergenic 50.0%
TGAGGTCCTCAAGCAAATGC+TGG + chr6_3:66900332-66900351 Msa0957100:CDS 50.0%
! GGGTTAGTACGCAAAGAGCT+TGG + chr6_3:66898634-66898653 Msa0957100:intron 50.0%
GTACGCAAAGAGCTTGGCAT+TGG + chr6_3:66898640-66898659 Msa0957100:intron 50.0%
ACGCAAAGAGCTTGGCATTG+GGG + chr6_3:66898642-66898661 Msa0957100:intron 50.0%
! GGCTTTGTCACACTTGCTCT+TGG + chr6_3:66898696-66898715 Msa0957100:intron 50.0%
CCCCACAATCAGAATTGCTC+AGG + chr6_3:66898755-66898774 Msa0957100:intron 50.0%
CATCAGCGGGTGTACCAAAA+GGG - chr6_3:66899256-66899275 None:intergenic 50.0%
ACATCAGCGGGTGTACCAAA+AGG - chr6_3:66899257-66899276 None:intergenic 50.0%
! TTTTGGTACACCCGCTGATG+TGG + chr6_3:66899256-66899275 Msa0957100:intron 50.0%
! AGAGAGAGAGAGGGTGTGTA+AGG - chr6_3:66899344-66899363 None:intergenic 50.0%
! GTACGATGGCCTTTTATGGC+TGG + chr6_3:66899942-66899961 Msa0957100:intron 50.0%
!! TTTAGGTGCCGAGGTTATGC+TGG + chr6_3:66900038-66900057 Msa0957100:intron 50.0%
CTTTCGCTACAACTACGCTC+AGG - chr6_3:66900195-66900214 None:intergenic 50.0%
!! AGTTGTAGCGAAAGAGCACC+TGG + chr6_3:66900201-66900220 Msa0957100:CDS 50.0%
!! GTTGTAGCGAAAGAGCACCT+GGG + chr6_3:66900202-66900221 Msa0957100:CDS 50.0%
TGGAGGCAATGCCGTTGTAA+CGG - chr6_3:66900254-66900273 None:intergenic 50.0%
TGAGGTCCTCAAGCAAATGC+TGG + chr6_3:66900332-66900351 Msa0957100:CDS 50.0%
CTGATTGTGGGGCTATCTGC+TGG - chr6_3:66898747-66898766 None:intergenic 55.0%
GCAAAGGATGCCACATCAGC+GGG - chr6_3:66899269-66899288 None:intergenic 55.0%
GGCAAAGGATGCCACATCAG+CGG - chr6_3:66899270-66899289 None:intergenic 55.0%
GCTGATGTGGCATCCTTTGC+CGG + chr6_3:66899269-66899288 Msa0957100:intron 55.0%
CTGATGTGGCATCCTTTGCC+GGG + chr6_3:66899270-66899289 Msa0957100:intron 55.0%
CCAGTTGCATCAGTCTTCCC+TGG - chr6_3:66900105-66900124 None:intergenic 55.0%
CCAGGGAAGACTGATGCAAC+TGG + chr6_3:66900102-66900121 Msa0957100:CDS 55.0%
GGGTGAGAATAACGCGTGCT+TGG - chr6_3:66900233-66900252 None:intergenic 55.0%
GGAGGCAATGCCGTTGTAAC+GGG - chr6_3:66900253-66900272 None:intergenic 55.0%
ACGGCATTGCCTCCAATGAC+AGG + chr6_3:66900259-66900278 Msa0957100:CDS 55.0%
ATGGCAGAGGAGGTTGCTTC+TGG + chr6_3:66900303-66900322 Msa0957100:CDS 55.0%
CTGATTGTGGGGCTATCTGC+TGG - chr6_3:66898747-66898766 None:intergenic 55.0%
GCAAAGGATGCCACATCAGC+GGG - chr6_3:66899269-66899288 None:intergenic 55.0%
GGCAAAGGATGCCACATCAG+CGG - chr6_3:66899270-66899289 None:intergenic 55.0%
GCTGATGTGGCATCCTTTGC+CGG + chr6_3:66899269-66899288 Msa0957100:intron 55.0%
CTGATGTGGCATCCTTTGCC+GGG + chr6_3:66899270-66899289 Msa0957100:intron 55.0%
CCAGTTGCATCAGTCTTCCC+TGG - chr6_3:66900105-66900124 None:intergenic 55.0%
CCAGGGAAGACTGATGCAAC+TGG + chr6_3:66900102-66900121 Msa0957100:CDS 55.0%
GGGTGAGAATAACGCGTGCT+TGG - chr6_3:66900233-66900252 None:intergenic 55.0%
GGAGGCAATGCCGTTGTAAC+GGG - chr6_3:66900253-66900272 None:intergenic 55.0%
ACGGCATTGCCTCCAATGAC+AGG + chr6_3:66900259-66900278 Msa0957100:CDS 55.0%
ATGGCAGAGGAGGTTGCTTC+TGG + chr6_3:66900303-66900322 Msa0957100:CDS 55.0%
ATGTGGCATCCTTTGCCGGG+TGG + chr6_3:66899273-66899292 Msa0957100:intron 60.0%
! GGTTGCTTCTGGCTGTGCTG+AGG + chr6_3:66900314-66900333 Msa0957100:CDS 60.0%
ATGTGGCATCCTTTGCCGGG+TGG + chr6_3:66899273-66899292 Msa0957100:intron 60.0%
! GGTTGCTTCTGGCTGTGCTG+AGG + chr6_3:66900314-66900333 Msa0957100:CDS 60.0%
ACGCGTGCTTGGTCACGCCC+AGG - chr6_3:66900222-66900241 None:intergenic 70.0%
ACGCGTGCTTGGTCACGCCC+AGG - chr6_3:66900222-66900241 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 66898507 66900617 66898507 ID=Msa0957100;Name=Msa0957100
chr6_3 mRNA 66898507 66900617 66898507 ID=Msa0957100-mRNA-1;Parent=Msa0957100;Name=Msa0957100-mRNA-1;_AED=0.14;_eAED=0.22;_QI=0|0|0.5|1|1|1|2|243|130
chr6_3 exon 66898507 66898567 66898507 ID=Msa0957100-mRNA-1:exon:12445;Parent=Msa0957100-mRNA-1
chr6_3 exon 66900043 66900617 66900043 ID=Msa0957100-mRNA-1:exon:12446;Parent=Msa0957100-mRNA-1
chr6_3 CDS 66898507 66898567 66898507 ID=Msa0957100-mRNA-1:cds;Parent=Msa0957100-mRNA-1
chr6_3 CDS 66900043 66900374 66900043 ID=Msa0957100-mRNA-1:cds;Parent=Msa0957100-mRNA-1
chr6_3 three_prime_UTR 66900375 66900617 66900375 ID=Msa0957100-mRNA-1:three_prime_utr;Parent=Msa0957100-mRNA-1
Gene Sequence

>Msa0957100

ATGGTTGATGGTGCTGACCAATTTGAAGAAAAGGATCCAAATGAGGCAAAAGCACTTGCAAGTGCCGAGGTTATGCTGGAATGCAAGCAAAGGAACAACAACGAAGTTGTTTTCTCAAAACCAGGGAAGACTGATGCAACTGGAGAATATACAATCTATGTGGATCAAGATCGCGCAGATGAAGTGTGTGATGTGAAGCTAATAAACACTCCTGAGCGTAGTTGTAGCGAAAGAGCACCTGGGCGTGACCAAGCACGCGTTATTCTCACCCGTTACAACGGCATTGCCTCCAATGACAGGTTTGCCAATGCTATGTTGTACATGGCAGAGGAGGTTGCTTCTGGCTGTGCTGAGGTCCTCAAGCAAATGCTGGAATTGGATGAAGAGAATTAA
Protein sequence

>Msa0957100

MVDGADQFEEKDPNEAKALASAEVMLECKQRNNNEVVFSKPGKTDATGEYTIYVDQDRADEVCDVKLINTPERSCSERAPGRDQARVILTRYNGIASNDRFANAMLYMAEEVASGCAEVLKQMLELDEEN*