AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0964430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0964430 MtrunA17_Chr6g0484671 72.477 109 17 3 17 113 144 251 4.22e-25 96.3
Msa0964430 MtrunA17_Chr6g0484671 59.375 128 21 6 18 115 186 312 1.77e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 56 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATGCTGCTAGTACTTA+TGG 0.182311 6_3:-90963826 Msa0964430:CDS
GCAGGCATCCATATATATTA+TGG 0.251658 6_3:+90963462 None:intergenic
AAAATATGTATGTTGTATTA+AGG 0.273872 6_3:-90963286 Msa0964430:three_prime_UTR
GGAGCTACCGGTGGCGGAAC+TGG 0.275507 6_3:-90963904 Msa0964430:CDS
AGGGATTGTGGACAAGATTA+AGG 0.287150 6_3:-90963518 Msa0964430:CDS
AAGATTAAGGAGAAGCTTCC+TGG 0.308792 6_3:-90963505 Msa0964430:CDS
ACACTAAACCATAATATATA+TGG 0.322019 6_3:-90963470 Msa0964430:exon
CCTGTTCCAGTTCCAGTTCC+AGG 0.353061 6_3:+90963715 None:intergenic
AAGATTCCTGGAACTGGAAC+TGG 0.359093 6_3:-90963721 Msa0964430:CDS
TCTCAATATCAACAAGGTTA+TGG 0.374924 6_3:-90963925 Msa0964430:CDS
AGGGGTAATGGACAAGATTA+AGG 0.379804 6_3:-90963869 Msa0964430:CDS
AAGATCAAGGAGAAGATTCC+TGG 0.387597 6_3:-90963733 Msa0964430:CDS
CCTGGAACTGGAACTGGAAC+AGG 0.387624 6_3:-90963715 Msa0964430:intron
AAGATTAAGGCGAAAATTCC+CGG 0.410456 6_3:-90963856 Msa0964430:CDS
AAAATGCTGCTAGTACTTAT+GGG 0.425979 6_3:-90963825 Msa0964430:CDS
AAGGAGAAGATTCCTGGAAC+TGG 0.429498 6_3:-90963727 Msa0964430:CDS
TGGAGATAATAGAGGAGTTA+TGG 0.444393 6_3:-90963758 Msa0964430:CDS
ATTAAGGAATTGATATATGT+TGG 0.448768 6_3:-90963270 Msa0964430:three_prime_UTR
CATCAACAACATGGAAAGAA+AGG 0.456958 6_3:-90963538 Msa0964430:CDS
GGAACAGGTCATGGAACAAC+TGG 0.460620 6_3:-90963583 Msa0964430:intron
GGGACAGGACATGGAACAAC+TGG 0.471797 6_3:-90963799 Msa0964430:CDS
GGTCATGGAACAACTGGTTA+TGG 0.473670 6_3:-90963577 Msa0964430:CDS
TAGTGTCCTTGTCCATGTCC+AGG 0.475946 6_3:+90963487 None:intergenic
GAGCTACCGGTGGCGGAACT+GGG 0.485254 6_3:-90963903 Msa0964430:CDS
GCATGATAATAGAGGGGTAA+TGG 0.494411 6_3:-90963881 Msa0964430:CDS
ATATATGAGGTGTATAATGA+TGG 0.499150 6_3:-90963324 Msa0964430:three_prime_UTR
AGGAGTTATGGACAAGATCA+AGG 0.502550 6_3:-90963746 Msa0964430:CDS
AGTACTTATGGGACAGGGAC+AGG 0.509114 6_3:-90963814 Msa0964430:CDS
GCTGCTAGTACTTATGGGAC+AGG 0.515021 6_3:-90963820 Msa0964430:CDS
TAGAATAAATATGTGTGTAT+TGG 0.515610 6_3:-90963420 Msa0964430:three_prime_UTR
GGTTATGGAGCTACCGGTGG+CGG 0.520450 6_3:-90963910 Msa0964430:CDS
GGAACTGGGCATGATAATAG+AGG 0.529404 6_3:-90963889 Msa0964430:CDS
CAACAAGGTTATGGAGCTAC+CGG 0.539969 6_3:-90963916 Msa0964430:CDS
GGTTATGGTGGTGTTGGTCA+TGG 0.545868 6_3:-90963562 Msa0964430:CDS
ATCAACAACATGGAAAGAAA+GGG 0.547780 6_3:-90963537 Msa0964430:CDS
CAACAACATGGAGATAATAG+AGG 0.548264 6_3:-90963766 Msa0964430:CDS
ATCATGCCCAGTTCCGCCAC+CGG 0.554198 6_3:+90963897 None:intergenic
TGATTGATCATATCAAATGC+AGG 0.563232 6_3:+90963444 None:intergenic
AAGGAGAAGCTTCCTGGACA+TGG 0.566266 6_3:-90963499 Msa0964430:CDS
TATATGAGGTGTATAATGAT+GGG 0.568794 6_3:-90963323 Msa0964430:three_prime_UTR
TATGGGACAGGGACAGGACA+TGG 0.569049 6_3:-90963808 Msa0964430:CDS
ACATGGAAAGAAAGGGATTG+TGG 0.578076 6_3:-90963530 Msa0964430:CDS
TATATATCTCTTACATGAGG+TGG 0.578862 6_3:+90963342 None:intergenic
GGACATGGAACAACTGGTGT+TGG 0.586251 6_3:-90963793 Msa0964430:CDS
TCATGTAAGAGATATATATG+AGG 0.593392 6_3:-90963337 Msa0964430:three_prime_UTR
ACAACTGGTTATGGTGGTGT+TGG 0.599005 6_3:-90963568 Msa0964430:CDS
AAGCTTCCTGGACATGGACA+AGG 0.605612 6_3:-90963493 Msa0964430:CDS
GAACTGGGCATGATAATAGA+GGG 0.607970 6_3:-90963888 Msa0964430:CDS
CTGCTAGTACTTATGGGACA+GGG 0.654209 6_3:-90963819 Msa0964430:CDS
TCATATATATCTCTTACATG+AGG 0.665718 6_3:+90963339 None:intergenic
CAAGGTTATGGAGCTACCGG+TGG 0.670970 6_3:-90963913 Msa0964430:CDS
GGTGTTGGTCATCAACAACA+TGG 0.679464 6_3:-90963778 Msa0964430:CDS
GGTCATGGTCATCAACAACA+TGG 0.689366 6_3:-90963547 Msa0964430:CDS
AACTGGGCATGATAATAGAG+GGG 0.696526 6_3:-90963887 Msa0964430:CDS
CATGGAACAACTGGTTATGG+TGG 0.707484 6_3:-90963574 Msa0964430:CDS
ACAATGTCTCAATATCAACA+AGG 0.721195 6_3:-90963931 Msa0964430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATGTATGTTGTATTA+AGG - chr6_3:90964012-90964031 Msa0964430:five_prime_UTR 15.0%
!! AAAATATGTATGTTGTATTA+AGG - chr6_3:90964012-90964031 Msa0964430:five_prime_UTR 15.0%
!! ACACTAAACCATAATATATA+TGG - chr6_3:90963828-90963847 Msa0964430:CDS 20.0%
!! TAGAATAAATATGTGTGTAT+TGG - chr6_3:90963878-90963897 Msa0964430:CDS 20.0%
!! ATTAAGGAATTGATATATGT+TGG - chr6_3:90964028-90964047 Msa0964430:five_prime_UTR 20.0%
!! ACACTAAACCATAATATATA+TGG - chr6_3:90963828-90963847 Msa0964430:CDS 20.0%
!! TAGAATAAATATGTGTGTAT+TGG - chr6_3:90963878-90963897 Msa0964430:CDS 20.0%
!! ATTAAGGAATTGATATATGT+TGG - chr6_3:90964028-90964047 Msa0964430:five_prime_UTR 20.0%
!!! TTTTGTTTTCTTGGTTTCTA+AGG + chr6_3:90963308-90963327 None:intergenic 25.0%
! TCATATATATCTCTTACATG+AGG + chr6_3:90963962-90963981 None:intergenic 25.0%
! TCATGTAAGAGATATATATG+AGG - chr6_3:90963961-90963980 Msa0964430:five_prime_UTR 25.0%
! ATATATGAGGTGTATAATGA+TGG - chr6_3:90963974-90963993 Msa0964430:five_prime_UTR 25.0%
! TATATGAGGTGTATAATGAT+GGG - chr6_3:90963975-90963994 Msa0964430:five_prime_UTR 25.0%
!!! TTTTGTTTTCTTGGTTTCTA+AGG + chr6_3:90963308-90963327 None:intergenic 25.0%
! TCATATATATCTCTTACATG+AGG + chr6_3:90963962-90963981 None:intergenic 25.0%
! TCATGTAAGAGATATATATG+AGG - chr6_3:90963961-90963980 Msa0964430:five_prime_UTR 25.0%
! ATATATGAGGTGTATAATGA+TGG - chr6_3:90963974-90963993 Msa0964430:five_prime_UTR 25.0%
! TATATGAGGTGTATAATGAT+GGG - chr6_3:90963975-90963994 Msa0964430:five_prime_UTR 25.0%
!!! AACGTGAAGTTTTGTTTTCT+TGG + chr6_3:90963317-90963336 None:intergenic 30.0%
ACAATGTCTCAATATCAACA+AGG - chr6_3:90963367-90963386 Msa0964430:three_prime_UTR 30.0%
TCTCAATATCAACAAGGTTA+TGG - chr6_3:90963373-90963392 Msa0964430:three_prime_UTR 30.0%
AAAATGCTGCTAGTACTTAT+GGG - chr6_3:90963473-90963492 Msa0964430:exon 30.0%
ATCAACAACATGGAAAGAAA+GGG - chr6_3:90963644-90963663 Msa0964430:intron 30.0%
ATCAACAACATGGAAAGAAA+GGG - chr6_3:90963644-90963663 Msa0964430:intron 30.0%
TGATTGATCATATCAAATGC+AGG + chr6_3:90963857-90963876 None:intergenic 30.0%
TATATATCTCTTACATGAGG+TGG + chr6_3:90963959-90963978 None:intergenic 30.0%
!!! AACGTGAAGTTTTGTTTTCT+TGG + chr6_3:90963317-90963336 None:intergenic 30.0%
ACAATGTCTCAATATCAACA+AGG - chr6_3:90963367-90963386 Msa0964430:three_prime_UTR 30.0%
TCTCAATATCAACAAGGTTA+TGG - chr6_3:90963373-90963392 Msa0964430:three_prime_UTR 30.0%
AAAATGCTGCTAGTACTTAT+GGG - chr6_3:90963473-90963492 Msa0964430:exon 30.0%
ATCAACAACATGGAAAGAAA+GGG - chr6_3:90963644-90963663 Msa0964430:intron 30.0%
ATCAACAACATGGAAAGAAA+GGG - chr6_3:90963644-90963663 Msa0964430:intron 30.0%
TGATTGATCATATCAAATGC+AGG + chr6_3:90963857-90963876 None:intergenic 30.0%
TATATATCTCTTACATGAGG+TGG + chr6_3:90963959-90963978 None:intergenic 30.0%
AAGATTAAGGCGAAAATTCC+CGG - chr6_3:90963442-90963461 Msa0964430:three_prime_UTR 35.0%
CAAAATGCTGCTAGTACTTA+TGG - chr6_3:90963472-90963491 Msa0964430:exon 35.0%
CAACAACATGGAGATAATAG+AGG - chr6_3:90963532-90963551 Msa0964430:CDS 35.0%
TGGAGATAATAGAGGAGTTA+TGG - chr6_3:90963540-90963559 Msa0964430:CDS 35.0%
CATCAACAACATGGAAAGAA+AGG - chr6_3:90963643-90963662 Msa0964430:intron 35.0%
TCAACAACATGGAAAGAAAG+GGG - chr6_3:90963645-90963664 Msa0964430:intron 35.0%
AAGATTAAGGAGAAGATTCC+TGG - chr6_3:90963676-90963695 Msa0964430:intron 35.0%
CATCAACAACATGGAAAGAA+AGG - chr6_3:90963643-90963662 Msa0964430:intron 35.0%
GCAGGCATCCATATATATTA+TGG + chr6_3:90963839-90963858 None:intergenic 35.0%
AAGATTAAGGCGAAAATTCC+CGG - chr6_3:90963442-90963461 Msa0964430:three_prime_UTR 35.0%
CAAAATGCTGCTAGTACTTA+TGG - chr6_3:90963472-90963491 Msa0964430:exon 35.0%
CAACAACATGGAGATAATAG+AGG - chr6_3:90963532-90963551 Msa0964430:CDS 35.0%
TGGAGATAATAGAGGAGTTA+TGG - chr6_3:90963540-90963559 Msa0964430:CDS 35.0%
CATCAACAACATGGAAAGAA+AGG - chr6_3:90963643-90963662 Msa0964430:intron 35.0%
TCAACAACATGGAAAGAAAG+GGG - chr6_3:90963645-90963664 Msa0964430:intron 35.0%
AAGATTAAGGAGAAGATTCC+TGG - chr6_3:90963676-90963695 Msa0964430:intron 35.0%
CATCAACAACATGGAAAGAA+AGG - chr6_3:90963643-90963662 Msa0964430:intron 35.0%
GCAGGCATCCATATATATTA+TGG + chr6_3:90963839-90963858 None:intergenic 35.0%
GAACTGGGCATGATAATAGA+GGG - chr6_3:90963410-90963429 Msa0964430:three_prime_UTR 40.0%
AACTGGGCATGATAATAGAG+GGG - chr6_3:90963411-90963430 Msa0964430:three_prime_UTR 40.0%
GCATGATAATAGAGGGGTAA+TGG - chr6_3:90963417-90963436 Msa0964430:three_prime_UTR 40.0%
!! AGGGGTAATGGACAAGATTA+AGG - chr6_3:90963429-90963448 Msa0964430:three_prime_UTR 40.0%
!! AGCAGCATTTTGATGAGTAC+CGG + chr6_3:90963464-90963483 None:intergenic 40.0%
AGGAGTTATGGACAAGATCA+AGG - chr6_3:90963552-90963571 Msa0964430:CDS 40.0%
AAGATCAAGGAGAAGATTCC+TGG - chr6_3:90963565-90963584 Msa0964430:CDS 40.0%
ACATGGAAAGAAAGGGATTG+TGG - chr6_3:90963768-90963787 Msa0964430:CDS 40.0%
AGGGATTGTGGACAAGATTA+AGG - chr6_3:90963780-90963799 Msa0964430:CDS 40.0%
AAGATTAAGGAGAAGCTTCC+TGG - chr6_3:90963793-90963812 Msa0964430:CDS 40.0%
GAACTGGGCATGATAATAGA+GGG - chr6_3:90963410-90963429 Msa0964430:three_prime_UTR 40.0%
AACTGGGCATGATAATAGAG+GGG - chr6_3:90963411-90963430 Msa0964430:three_prime_UTR 40.0%
GCATGATAATAGAGGGGTAA+TGG - chr6_3:90963417-90963436 Msa0964430:three_prime_UTR 40.0%
!! AGGGGTAATGGACAAGATTA+AGG - chr6_3:90963429-90963448 Msa0964430:three_prime_UTR 40.0%
!! AGCAGCATTTTGATGAGTAC+CGG + chr6_3:90963464-90963483 None:intergenic 40.0%
AGGAGTTATGGACAAGATCA+AGG - chr6_3:90963552-90963571 Msa0964430:CDS 40.0%
AAGATCAAGGAGAAGATTCC+TGG - chr6_3:90963565-90963584 Msa0964430:CDS 40.0%
ACATGGAAAGAAAGGGATTG+TGG - chr6_3:90963768-90963787 Msa0964430:CDS 40.0%
AGGGATTGTGGACAAGATTA+AGG - chr6_3:90963780-90963799 Msa0964430:CDS 40.0%
AAGATTAAGGAGAAGCTTCC+TGG - chr6_3:90963793-90963812 Msa0964430:CDS 40.0%
CAACAAGGTTATGGAGCTAC+CGG - chr6_3:90963382-90963401 Msa0964430:three_prime_UTR 45.0%
GGAACTGGGCATGATAATAG+AGG - chr6_3:90963409-90963428 Msa0964430:three_prime_UTR 45.0%
!! GCAGCATTTTGATGAGTACC+GGG + chr6_3:90963463-90963482 None:intergenic 45.0%
CTGCTAGTACTTATGGGACA+GGG - chr6_3:90963479-90963498 Msa0964430:exon 45.0%
!! GGTGTTGGTCATCAACAACA+TGG - chr6_3:90963520-90963539 Msa0964430:CDS 45.0%
AAGGAGAAGATTCCTGGAAC+TGG - chr6_3:90963571-90963590 Msa0964430:CDS 45.0%
AAGATTCCTGGAACTGGAAC+TGG - chr6_3:90963577-90963596 Msa0964430:CDS 45.0%
GGTCATGGAACAACTGGTTA+TGG - chr6_3:90963604-90963623 Msa0964430:intron 45.0%
GGTCATGGTCATCAACAACA+TGG - chr6_3:90963634-90963653 Msa0964430:intron 45.0%
ACATGGAAAGAAAGGGGTTG+TGG - chr6_3:90963651-90963670 Msa0964430:intron 45.0%
AGGGGTTGTGGACAAGATTA+AGG - chr6_3:90963663-90963682 Msa0964430:intron 45.0%
GGTCATGGAACAACTGGTTA+TGG - chr6_3:90963604-90963623 Msa0964430:intron 45.0%
CATGGAACAACTGGTTATGG+TGG - chr6_3:90963607-90963626 Msa0964430:intron 45.0%
! ACAACTGGTTATGGTGGTGT+TGG - chr6_3:90963730-90963749 Msa0964430:CDS 45.0%
GGTCATGGTCATCAACAACA+TGG - chr6_3:90963634-90963653 Msa0964430:intron 45.0%
CAACAAGGTTATGGAGCTAC+CGG - chr6_3:90963382-90963401 Msa0964430:three_prime_UTR 45.0%
GGAACTGGGCATGATAATAG+AGG - chr6_3:90963409-90963428 Msa0964430:three_prime_UTR 45.0%
!! GCAGCATTTTGATGAGTACC+GGG + chr6_3:90963463-90963482 None:intergenic 45.0%
CTGCTAGTACTTATGGGACA+GGG - chr6_3:90963479-90963498 Msa0964430:exon 45.0%
!! GGTGTTGGTCATCAACAACA+TGG - chr6_3:90963520-90963539 Msa0964430:CDS 45.0%
AAGGAGAAGATTCCTGGAAC+TGG - chr6_3:90963571-90963590 Msa0964430:CDS 45.0%
AAGATTCCTGGAACTGGAAC+TGG - chr6_3:90963577-90963596 Msa0964430:CDS 45.0%
GGTCATGGAACAACTGGTTA+TGG - chr6_3:90963604-90963623 Msa0964430:intron 45.0%
GGTCATGGTCATCAACAACA+TGG - chr6_3:90963634-90963653 Msa0964430:intron 45.0%
ACATGGAAAGAAAGGGGTTG+TGG - chr6_3:90963651-90963670 Msa0964430:intron 45.0%
AGGGGTTGTGGACAAGATTA+AGG - chr6_3:90963663-90963682 Msa0964430:intron 45.0%
GGTCATGGAACAACTGGTTA+TGG - chr6_3:90963604-90963623 Msa0964430:intron 45.0%
CATGGAACAACTGGTTATGG+TGG - chr6_3:90963607-90963626 Msa0964430:intron 45.0%
! ACAACTGGTTATGGTGGTGT+TGG - chr6_3:90963730-90963749 Msa0964430:CDS 45.0%
GGTCATGGTCATCAACAACA+TGG - chr6_3:90963634-90963653 Msa0964430:intron 45.0%
GCTGCTAGTACTTATGGGAC+AGG - chr6_3:90963478-90963497 Msa0964430:exon 50.0%
AGTACTTATGGGACAGGGAC+AGG - chr6_3:90963484-90963503 Msa0964430:exon 50.0%
GGACATGGAACAACTGGTGT+TGG - chr6_3:90963505-90963524 Msa0964430:CDS 50.0%
ACTGGAACTGGAACAGGTCA+TGG - chr6_3:90963589-90963608 Msa0964430:intron 50.0%
GGAACAGGTCATGGAACAAC+TGG - chr6_3:90963598-90963617 Msa0964430:intron 50.0%
GGAACAACTGGTTATGGTGC+TGG - chr6_3:90963610-90963629 Msa0964430:intron 50.0%
! ACAACTGGTTATGGTGCTGG+TGG - chr6_3:90963613-90963632 Msa0964430:intron 50.0%
AAGGAGAAGATTCCTGGCAC+TGG - chr6_3:90963682-90963701 Msa0964430:intron 50.0%
! AAGATTCCTGGCACTGGAAC+TGG - chr6_3:90963688-90963707 Msa0964430:intron 50.0%
ACTGGAACTGGAACTGGAAC+AGG - chr6_3:90963700-90963719 Msa0964430:intron 50.0%
ACTGGAACTGGAACAGGTCA+TGG - chr6_3:90963589-90963608 Msa0964430:intron 50.0%
GGAACAGGTCATGGAACAAC+TGG - chr6_3:90963598-90963617 Msa0964430:intron 50.0%
!! GGTTATGGTGGTGTTGGTCA+TGG - chr6_3:90963736-90963755 Msa0964430:CDS 50.0%
AAGGAGAAGCTTCCTGGACA+TGG - chr6_3:90963799-90963818 Msa0964430:CDS 50.0%
AAGCTTCCTGGACATGGACA+AGG - chr6_3:90963805-90963824 Msa0964430:CDS 50.0%
TAGTGTCCTTGTCCATGTCC+AGG + chr6_3:90963814-90963833 None:intergenic 50.0%
GCTGCTAGTACTTATGGGAC+AGG - chr6_3:90963478-90963497 Msa0964430:exon 50.0%
AGTACTTATGGGACAGGGAC+AGG - chr6_3:90963484-90963503 Msa0964430:exon 50.0%
GGACATGGAACAACTGGTGT+TGG - chr6_3:90963505-90963524 Msa0964430:CDS 50.0%
ACTGGAACTGGAACAGGTCA+TGG - chr6_3:90963589-90963608 Msa0964430:intron 50.0%
GGAACAGGTCATGGAACAAC+TGG - chr6_3:90963598-90963617 Msa0964430:intron 50.0%
GGAACAACTGGTTATGGTGC+TGG - chr6_3:90963610-90963629 Msa0964430:intron 50.0%
! ACAACTGGTTATGGTGCTGG+TGG - chr6_3:90963613-90963632 Msa0964430:intron 50.0%
AAGGAGAAGATTCCTGGCAC+TGG - chr6_3:90963682-90963701 Msa0964430:intron 50.0%
! AAGATTCCTGGCACTGGAAC+TGG - chr6_3:90963688-90963707 Msa0964430:intron 50.0%
ACTGGAACTGGAACTGGAAC+AGG - chr6_3:90963700-90963719 Msa0964430:intron 50.0%
ACTGGAACTGGAACAGGTCA+TGG - chr6_3:90963589-90963608 Msa0964430:intron 50.0%
GGAACAGGTCATGGAACAAC+TGG - chr6_3:90963598-90963617 Msa0964430:intron 50.0%
!! GGTTATGGTGGTGTTGGTCA+TGG - chr6_3:90963736-90963755 Msa0964430:CDS 50.0%
AAGGAGAAGCTTCCTGGACA+TGG - chr6_3:90963799-90963818 Msa0964430:CDS 50.0%
AAGCTTCCTGGACATGGACA+AGG - chr6_3:90963805-90963824 Msa0964430:CDS 50.0%
TAGTGTCCTTGTCCATGTCC+AGG + chr6_3:90963814-90963833 None:intergenic 50.0%
CAAGGTTATGGAGCTACCGG+TGG - chr6_3:90963385-90963404 Msa0964430:three_prime_UTR 55.0%
TATGGGACAGGGACAGGACA+TGG - chr6_3:90963490-90963509 Msa0964430:CDS 55.0%
GGGACAGGACATGGAACAAC+TGG - chr6_3:90963499-90963518 Msa0964430:CDS 55.0%
CCTGTTCCAGTTCCAGTTCC+AGG + chr6_3:90963586-90963605 None:intergenic 55.0%
CCTGGAACTGGAACTGGAAC+AGG - chr6_3:90963583-90963602 Msa0964430:intron 55.0%
!! GGTTATGGTGCTGGTGGTCA+TGG - chr6_3:90963619-90963638 Msa0964430:intron 55.0%
CAAGGTTATGGAGCTACCGG+TGG - chr6_3:90963385-90963404 Msa0964430:three_prime_UTR 55.0%
TATGGGACAGGGACAGGACA+TGG - chr6_3:90963490-90963509 Msa0964430:CDS 55.0%
GGGACAGGACATGGAACAAC+TGG - chr6_3:90963499-90963518 Msa0964430:CDS 55.0%
CCTGTTCCAGTTCCAGTTCC+AGG + chr6_3:90963586-90963605 None:intergenic 55.0%
CCTGGAACTGGAACTGGAAC+AGG - chr6_3:90963583-90963602 Msa0964430:intron 55.0%
!! GGTTATGGTGCTGGTGGTCA+TGG - chr6_3:90963619-90963638 Msa0964430:intron 55.0%
GGTTATGGAGCTACCGGTGG+CGG - chr6_3:90963388-90963407 Msa0964430:three_prime_UTR 60.0%
ATCATGCCCAGTTCCGCCAC+CGG + chr6_3:90963404-90963423 None:intergenic 60.0%
CCAGTTCCAGTTCCAGTGCC+AGG + chr6_3:90963697-90963716 None:intergenic 60.0%
! CCTGGCACTGGAACTGGAAC+TGG - chr6_3:90963694-90963713 Msa0964430:intron 60.0%
GGTTATGGAGCTACCGGTGG+CGG - chr6_3:90963388-90963407 Msa0964430:three_prime_UTR 60.0%
ATCATGCCCAGTTCCGCCAC+CGG + chr6_3:90963404-90963423 None:intergenic 60.0%
CCAGTTCCAGTTCCAGTGCC+AGG + chr6_3:90963697-90963716 None:intergenic 60.0%
! CCTGGCACTGGAACTGGAAC+TGG - chr6_3:90963694-90963713 Msa0964430:intron 60.0%
!! GAGCTACCGGTGGCGGAACT+GGG - chr6_3:90963395-90963414 Msa0964430:three_prime_UTR 65.0%
!! GAGCTACCGGTGGCGGAACT+GGG - chr6_3:90963395-90963414 Msa0964430:three_prime_UTR 65.0%
!! GGAGCTACCGGTGGCGGAAC+TGG - chr6_3:90963394-90963413 Msa0964430:three_prime_UTR 70.0%
!! GGAGCTACCGGTGGCGGAAC+TGG - chr6_3:90963394-90963413 Msa0964430:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 90963259 90964061 90963259 ID=Msa0964430;Name=Msa0964430
chr6_3 mRNA 90963259 90964061 90963259 ID=Msa0964430-mRNA-1;Parent=Msa0964430;Name=Msa0964430-mRNA-1;_AED=0.07;_eAED=0.07;_QI=111|1|1|1|0|0|2|227|115
chr6_3 exon 90963259 90963598 90963259 ID=Msa0964430-mRNA-1:exon:16406;Parent=Msa0964430-mRNA-1
chr6_3 exon 90963716 90964061 90963716 ID=Msa0964430-mRNA-1:exon:16405;Parent=Msa0964430-mRNA-1
chr6_3 five_prime_UTR 90963951 90964061 90963951 ID=Msa0964430-mRNA-1:five_prime_utr;Parent=Msa0964430-mRNA-1
chr6_3 CDS 90963716 90963950 90963716 ID=Msa0964430-mRNA-1:cds;Parent=Msa0964430-mRNA-1
chr6_3 CDS 90963486 90963598 90963486 ID=Msa0964430-mRNA-1:cds;Parent=Msa0964430-mRNA-1
chr6_3 three_prime_UTR 90963259 90963485 90963259 ID=Msa0964430-mRNA-1:three_prime_utr;Parent=Msa0964430-mRNA-1
Gene Sequence

>Msa0964430

ATGTCTCAATATCAACAAGGTTATGGAGCTACCGGTGGCGGAACTGGGCATGATAATAGAGGGGTAATGGACAAGATTAAGGCGAAAATTCCCGGTACTCATCAAAATGCTGCTAGTACTTATGGGACAGGGACAGGACATGGAACAACTGGTGTTGGTCATCAACAACATGGAGATAATAGAGGAGTTATGGACAAGATCAAGGAGAAGATTCCTGGAACTGGAACTGGAACAGGTCATGGAACAACTGGTTATGGTGGTGTTGGTCATGGTCATCAACAACATGGAAAGAAAGGGATTGTGGACAAGATTAAGGAGAAGCTTCCTGGACATGGACAAGGACACTAA
Protein sequence

>Msa0964430

MSQYQQGYGATGGGTGHDNRGVMDKIKAKIPGTHQNAASTYGTGTGHGTTGVGHQQHGDNRGVMDKIKEKIPGTGTGTGHGTTGYGGVGHGHQQHGKKGIVDKIKEKLPGHGQGH*