AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0983500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0983500 MtrunA17_MTg0491251 98.496 133 2 0 1 133 81 213 1.16e-92 267
Msa0983500 MtrunA17_Chr7g0231341 97.744 133 3 0 1 133 49 181 7.61e-92 265
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCCGACGCCGACCCATTT+TGG 0.210676 6_4:-26619960 None:intergenic
CTCTTTGGATGTATCCTTCT+GGG 0.227156 6_4:+26619860 None:intergenic
CCTCTTTGGATGTATCCTTC+TGG 0.235353 6_4:+26619859 None:intergenic
AGACTTCGTTGCAAATAAAT+TGG 0.238736 6_4:+26620080 Msa0983500:CDS
CCCGCCGCTCCTATCCAAAA+TGG 0.239934 6_4:+26619946 Msa0983500:CDS
CGTAGCGCTTGCTCATAAAT+TGG 0.318064 6_4:-26620126 None:intergenic
ATTGGACCCTGCGGCTGCCC+CGG 0.373068 6_4:-26620108 None:intergenic
GATAGGAGCGGCGGGTCCGC+CGG 0.375170 6_4:-26619938 None:intergenic
TATCCTTCTGGGGCTGATGC+TGG 0.380202 6_4:+26619871 Msa0983500:CDS
AGCGGCGGGTCCGCCGGGGC+GGG 0.390016 6_4:-26619932 None:intergenic
TATGGTTTCGCGGCCGGAGC+TGG 0.394590 6_4:-26620003 None:intergenic
GGGAAGGCTGATGCTGCTCT+TGG 0.402499 6_4:-26619912 None:intergenic
GCGGGTCCGCCGGGGCGGGA+AGG 0.403262 6_4:-26619928 None:intergenic
TTGGACCCTGCGGCTGCCCC+GGG 0.438206 6_4:-26620107 None:intergenic
GGAAGACCCGGGGCAGCCGC+AGG 0.439885 6_4:+26620101 Msa0983500:CDS
TAGGAGCGGCGGGTCCGCCG+GGG 0.472146 6_4:-26619936 None:intergenic
TTCCGTGGAGCAGCCAGCTC+CGG 0.472407 6_4:+26619990 Msa0983500:CDS
GGCCGGAGCTGGCTGCTCCA+CGG 0.474217 6_4:-26619992 None:intergenic
TGCAAATAAATTGGAAGACC+CGG 0.488016 6_4:+26620089 Msa0983500:CDS
CAGCATCAGCCTTCCCGCCC+CGG 0.489074 6_4:+26619919 Msa0983500:CDS
CCGCCGCTCCTATCCAAAAT+GGG 0.493631 6_4:+26619947 Msa0983500:CDS
GGACGAACTTCAGCGCTGGC+TGG 0.506906 6_4:+26620191 Msa0983500:CDS
AGCGCTGGCTGGTTTCACTA+AGG 0.509513 6_4:+26620202 Msa0983500:CDS
AGCTGAAGGAGGGCTATGTA+TGG 0.518841 6_4:-26620021 None:intergenic
GCGCTGGCTGGTTTCACTAA+GGG 0.521276 6_4:+26620203 Msa0983500:CDS
GCTATGTATGGTTTCGCGGC+CGG 0.522221 6_4:-26620009 None:intergenic
GAGGGCTATGTATGGTTTCG+CGG 0.534222 6_4:-26620013 None:intergenic
AGCAAGCGCTACGCCTGGTA+TGG 0.535586 6_4:+26620136 Msa0983500:CDS
TTATGAGCAAGCGCTACGCC+TGG 0.557111 6_4:+26620131 Msa0983500:CDS
CGCTCCTATCCAAAATGGGT+CGG 0.568858 6_4:+26619951 Msa0983500:CDS
GATCGATGGCAGTACTAACC+AGG 0.569954 6_4:+26620170 Msa0983500:CDS
GCAAATAAATTGGAAGACCC+GGG 0.573068 6_4:+26620090 Msa0983500:CDS
ATAGCCCTCCTTCAGCTAGA+GGG 0.580354 6_4:+26620027 Msa0983500:CDS
ACCAGGACGAACTTCAGCGC+TGG 0.584629 6_4:+26620187 Msa0983500:CDS
GAAGACCCGGGGCAGCCGCA+GGG 0.585751 6_4:+26620102 Msa0983500:CDS
GCCAGCGCTGAAGTTCGTCC+TGG 0.586980 6_4:-26620188 None:intergenic
TCTTTGGATGTATCCTTCTG+GGG 0.587601 6_4:+26619861 None:intergenic
TGGCTGCTCCACGGAACTAG+TGG 0.588921 6_4:-26619983 None:intergenic
AAAATGGATTTGTATTGTCG+GGG 0.604607 6_4:-26620231 None:intergenic
TCTCGAGTTCATACCATACC+AGG 0.605945 6_4:-26620149 None:intergenic
TGGTATGAACTCGAGATCGA+TGG 0.622194 6_4:+26620156 Msa0983500:CDS
TAGCCCTCCTTCAGCTAGAG+GGG 0.628752 6_4:+26620028 Msa0983500:CDS
TATCCAAAATGGGTCGGCGT+CGG 0.631571 6_4:+26619957 Msa0983500:CDS
GAGCCAGCATCAGCCCCAGA+AGG 0.632838 6_4:-26619874 None:intergenic
TGCTCATAAATTGGACCCTG+CGG 0.637696 6_4:-26620117 None:intergenic
CATAGCCCTCCTTCAGCTAG+AGG 0.642446 6_4:+26620026 Msa0983500:CDS
AGCCCTCCTTCAGCTAGAGG+GGG 0.644919 6_4:+26620029 Msa0983500:CDS
AAATGGATTTGTATTGTCGG+GGG 0.651927 6_4:-26620230 None:intergenic
CAAATAAATTGGAAGACCCG+GGG 0.684347 6_4:+26620091 Msa0983500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATGGATTTGTATTGTC+GGG - chr6_4:26620235-26620254 None:intergenic 25.0%
!! CAAAAATGGATTTGTATTGT+CGG - chr6_4:26620236-26620255 None:intergenic 25.0%
AGACTTCGTTGCAAATAAAT+TGG + chr6_4:26620080-26620099 Msa0983500:CDS 30.0%
! AAAATGGATTTGTATTGTCG+GGG - chr6_4:26620234-26620253 None:intergenic 30.0%
! CGACAATACAAATCCATTTT+TGG + chr6_4:26620234-26620253 Msa0983500:CDS 30.0%
TGCAAATAAATTGGAAGACC+CGG + chr6_4:26620089-26620108 Msa0983500:CDS 35.0%
! AAATGGATTTGTATTGTCGG+GGG - chr6_4:26620233-26620252 None:intergenic 35.0%
GCAAATAAATTGGAAGACCC+GGG + chr6_4:26620090-26620109 Msa0983500:CDS 40.0%
CAAATAAATTGGAAGACCCG+GGG + chr6_4:26620091-26620110 Msa0983500:CDS 40.0%
!!! AATCCATTTTTGGCTTCTCC+CGG + chr6_4:26620244-26620263 Msa0983500:CDS 40.0%
TGCTCATAAATTGGACCCTG+CGG - chr6_4:26620120-26620139 None:intergenic 45.0%
CGTAGCGCTTGCTCATAAAT+TGG - chr6_4:26620129-26620148 None:intergenic 45.0%
TCTCGAGTTCATACCATACC+AGG - chr6_4:26620152-26620171 None:intergenic 45.0%
TGGTATGAACTCGAGATCGA+TGG + chr6_4:26620156-26620175 Msa0983500:CDS 45.0%
!!! ATCCATTTTTGGCTTCTCCC+GGG + chr6_4:26620245-26620264 Msa0983500:CDS 45.0%
! CGACCCATTTTGGATAGGAG+CGG - chr6_4:26619953-26619972 None:intergenic 50.0%
CGCTCCTATCCAAAATGGGT+CGG + chr6_4:26619951-26619970 Msa0983500:CDS 50.0%
TATCCAAAATGGGTCGGCGT+CGG + chr6_4:26619957-26619976 Msa0983500:CDS 50.0%
GAGGGCTATGTATGGTTTCG+CGG - chr6_4:26620016-26620035 None:intergenic 50.0%
AGCTGAAGGAGGGCTATGTA+TGG - chr6_4:26620024-26620043 None:intergenic 50.0%
ATAGCCCTCCTTCAGCTAGA+GGG + chr6_4:26620027-26620046 Msa0983500:CDS 50.0%
GATCGATGGCAGTACTAACC+AGG + chr6_4:26620170-26620189 Msa0983500:CDS 50.0%
!!! TCCATTTTTGGCTTCTCCCG+GGG + chr6_4:26620246-26620265 Msa0983500:CDS 50.0%
!! TATCCTTCTGGGGCTGATGC+TGG + chr6_4:26619871-26619890 Msa0983500:CDS 55.0%
! CCATTTTGGATAGGAGCGGC+GGG - chr6_4:26619949-26619968 None:intergenic 55.0%
! CCCATTTTGGATAGGAGCGG+CGG - chr6_4:26619950-26619969 None:intergenic 55.0%
CCGCCGCTCCTATCCAAAAT+GGG + chr6_4:26619947-26619966 Msa0983500:CDS 55.0%
! GACGCCGACCCATTTTGGAT+AGG - chr6_4:26619958-26619977 None:intergenic 55.0%
GCTATGTATGGTTTCGCGGC+CGG - chr6_4:26620012-26620031 None:intergenic 55.0%
CATAGCCCTCCTTCAGCTAG+AGG + chr6_4:26620026-26620045 Msa0983500:CDS 55.0%
TAGCCCTCCTTCAGCTAGAG+GGG + chr6_4:26620028-26620047 Msa0983500:CDS 55.0%
TTATGAGCAAGCGCTACGCC+TGG + chr6_4:26620131-26620150 Msa0983500:CDS 55.0%
!! AGCGCTGGCTGGTTTCACTA+AGG + chr6_4:26620202-26620221 Msa0983500:CDS 55.0%
!! GCGCTGGCTGGTTTCACTAA+GGG + chr6_4:26620203-26620222 Msa0983500:CDS 55.0%
!!! CCATTTTTGGCTTCTCCCGG+GGG + chr6_4:26620247-26620266 Msa0983500:CDS 55.0%
!!! CATTTTTGGCTTCTCCCGGG+GGG + chr6_4:26620248-26620267 Msa0983500:CDS 55.0%
GGGAAGGCTGATGCTGCTCT+TGG - chr6_4:26619915-26619934 None:intergenic 60.0%
CCCGCCGCTCCTATCCAAAA+TGG + chr6_4:26619946-26619965 Msa0983500:CDS 60.0%
! AAGCCGACGCCGACCCATTT+TGG - chr6_4:26619963-26619982 None:intergenic 60.0%
! GTCGGCTTCCACTAGTTCCG+TGG + chr6_4:26619975-26619994 Msa0983500:CDS 60.0%
TGGCTGCTCCACGGAACTAG+TGG - chr6_4:26619986-26620005 None:intergenic 60.0%
AGCCCTCCTTCAGCTAGAGG+GGG + chr6_4:26620029-26620048 Msa0983500:CDS 60.0%
CTCCCCCTCTAGCTGAAGGA+GGG - chr6_4:26620034-26620053 None:intergenic 60.0%
TCCTTCAGCTAGAGGGGGAG+CGG + chr6_4:26620034-26620053 Msa0983500:CDS 60.0%
AGCAAGCGCTACGCCTGGTA+TGG + chr6_4:26620136-26620155 Msa0983500:CDS 60.0%
ACCAGGACGAACTTCAGCGC+TGG + chr6_4:26620187-26620206 Msa0983500:CDS 60.0%
CCCCCGGGAGAAGCCAAAAA+TGG - chr6_4:26620250-26620269 None:intergenic 60.0%
GAGCCAGCATCAGCCCCAGA+AGG - chr6_4:26619877-26619896 None:intergenic 65.0%
TTCCGTGGAGCAGCCAGCTC+CGG + chr6_4:26619990-26620009 Msa0983500:CDS 65.0%
TATGGTTTCGCGGCCGGAGC+TGG - chr6_4:26620006-26620025 None:intergenic 65.0%
GCTCCCCCTCTAGCTGAAGG+AGG - chr6_4:26620035-26620054 None:intergenic 65.0%
TCCGCTCCCCCTCTAGCTGA+AGG - chr6_4:26620038-26620057 None:intergenic 65.0%
! GCCAGCGCTGAAGTTCGTCC+TGG - chr6_4:26620191-26620210 None:intergenic 65.0%
!! GGACGAACTTCAGCGCTGGC+TGG + chr6_4:26620191-26620210 Msa0983500:CDS 65.0%
CAGCATCAGCCTTCCCGCCC+CGG + chr6_4:26619919-26619938 Msa0983500:CDS 70.0%
ATTGGACCCTGCGGCTGCCC+CGG - chr6_4:26620111-26620130 None:intergenic 70.0%
CATCAGCCTTCCCGCCCCGG+CGG + chr6_4:26619922-26619941 Msa0983500:CDS 75.0%
ATAGGAGCGGCGGGTCCGCC+GGG - chr6_4:26619940-26619959 None:intergenic 75.0%
GATAGGAGCGGCGGGTCCGC+CGG - chr6_4:26619941-26619960 None:intergenic 75.0%
GGCCGGAGCTGGCTGCTCCA+CGG - chr6_4:26619995-26620014 None:intergenic 75.0%
GAAGACCCGGGGCAGCCGCA+GGG + chr6_4:26620102-26620121 Msa0983500:CDS 75.0%
TTGGACCCTGCGGCTGCCCC+GGG - chr6_4:26620110-26620129 None:intergenic 75.0%
! TAGGAGCGGCGGGTCCGCCG+GGG - chr6_4:26619939-26619958 None:intergenic 80.0%
! GGAAGACCCGGGGCAGCCGC+AGG + chr6_4:26620101-26620120 Msa0983500:CDS 80.0%
! GCGGGTCCGCCGGGGCGGGA+AGG - chr6_4:26619931-26619950 None:intergenic 90.0%
! AGCGGCGGGTCCGCCGGGGC+GGG - chr6_4:26619935-26619954 None:intergenic 90.0%
! GAGCGGCGGGTCCGCCGGGG+CGG - chr6_4:26619936-26619955 None:intergenic 90.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 26619868 26620281 26619868 ID=Msa0983500;Name=Msa0983500
chr6_4 mRNA 26619868 26620281 26619868 ID=Msa0983500-mRNA-1;Parent=Msa0983500;Name=Msa0983500-mRNA-1;_AED=0.26;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|137
chr6_4 exon 26619868 26620281 26619868 ID=Msa0983500-mRNA-1:exon:7515;Parent=Msa0983500-mRNA-1
chr6_4 CDS 26619868 26620281 26619868 ID=Msa0983500-mRNA-1:cds;Parent=Msa0983500-mRNA-1
Gene Sequence

>Msa0983500

ATGTATCCTTCTGGGGCTGATGCTGGCTCAGAAGCAAGCGTAAACCAAGAGCAGCATCAGCCTTCCCGCCCCGGCGGACCCGCCGCTCCTATCCAAAATGGGTCGGCGTCGGCTTCCACTAGTTCCGTGGAGCAGCCAGCTCCGGCCGCGAAACCATACATAGCCCTCCTTCAGCTAGAGGGGGAGCGGAAACGTCTTATCGACGATATTGTAGACTTCGTTGCAAATAAATTGGAAGACCCGGGGCAGCCGCAGGGTCCAATTTATGAGCAAGCGCTACGCCTGGTATGGTATGAACTCGAGATCGATGGCAGTACTAACCAGGACGAACTTCAGCGCTGGCTGGTTTCACTAAGGGAAAACCCCCGACAATACAAATCCATTTTTGGCTTCTCCCGGGGGGATTTTTTATGA

Protein sequence

>Msa0983500

MYPSGADAGSEASVNQEQHQPSRPGGPAAPIQNGSASASTSSVEQPAPAAKPYIALLQLEGERKRLIDDIVDFVANKLEDPGQPQGPIYEQALRLVWYELEIDGSTNQDELQRWLVSLRENPRQYKSIFGFSRGDFL*