AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1019270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1019270 MtrunA17_Chr7g0234821 96.963 428 13 0 25 452 25 452 0.0 866
Msa1019270 MtrunA17_Chr8g0339191 70.796 452 127 2 1 452 6 452 0.0 684
Msa1019270 MtrunA17_Chr4g0046311 54.267 457 199 3 1 454 4 453 0.0 546
Msa1019270 MtrunA17_Chr1g0177171 41.215 461 237 8 27 463 39 489 6.61e-119 357
Msa1019270 MtrunA17_Chr5g0439251 40.757 449 249 5 27 462 26 470 4.29e-118 355
Msa1019270 MtrunA17_Chr6g0487261 39.868 454 256 9 4 452 7 448 6.39e-111 337
Msa1019270 MtrunA17_Chr8g0389741 39.813 427 247 6 27 452 29 446 3.71e-108 330
Msa1019270 MtrunA17_Chr4g0034901 36.830 429 263 6 27 452 30 453 4.70e-98 303
Msa1019270 MtrunA17_Chr4g0003281 42.297 357 194 6 3 355 18 366 6.36e-98 300
Msa1019270 MtrunA17_Chr3g0144531 36.923 455 267 9 4 452 8 448 1.71e-95 296
Msa1019270 MtrunA17_Chr3g0110371 34.279 458 276 8 1 453 3 440 5.63e-90 283
Msa1019270 MtrunA17_Chr4g0026321 41.018 334 191 5 27 359 30 358 3.36e-89 277
Msa1019270 MtrunA17_Chr4g0043851 43.385 325 178 5 27 350 33 352 6.34e-89 277
Msa1019270 MtrunA17_Chr4g0075031 36.977 430 254 7 27 453 46 461 6.20e-88 278
Msa1019270 MtrunA17_Chr3g0119541 41.896 327 185 4 21 346 21 343 5.55e-87 271
Msa1019270 MtrunA17_Chr8g0378621 34.033 429 267 6 27 452 27 442 8.03e-87 275
Msa1019270 MtrunA17_Chr2g0285901 41.092 348 198 5 1 348 1 341 1.50e-86 271
Msa1019270 MtrunA17_Chr2g0321511 40.762 341 183 8 27 360 30 358 5.49e-86 269
Msa1019270 MtrunA17_Chr1g0161011 32.895 456 287 8 1 453 1 440 1.41e-85 271
Msa1019270 MtrunA17_Chr5g0434591 33.551 459 279 10 1 453 1 439 8.41e-85 269
Msa1019270 MtrunA17_Chr3g0121271 32.969 458 276 10 2 453 9 441 1.84e-84 268
Msa1019270 MtrunA17_Chr1g0146831 45.171 321 166 6 27 345 32 344 1.80e-83 262
Msa1019270 MtrunA17_Chr4g0043511 36.564 454 262 11 18 452 51 497 2.48e-83 267
Msa1019270 MtrunA17_Chr7g0223961 37.788 434 249 11 27 453 24 443 1.09e-81 259
Msa1019270 MtrunA17_Chr1g0189111 41.667 324 182 5 27 349 32 349 1.50e-81 258
Msa1019270 MtrunA17_Chr4g0064021 36.716 335 207 4 17 350 17 347 3.14e-79 251
Msa1019270 MtrunA17_Chr1g0155001 37.846 325 196 5 27 349 25 345 1.24e-70 230
Msa1019270 MtrunA17_Chr2g0294591 35.650 331 199 8 23 351 27 345 9.21e-67 218
Msa1019270 MtrunA17_Chr2g0294601 36.080 352 204 11 3 351 41 374 7.91e-66 217
Msa1019270 MtrunA17_Chr4g0038981 36.646 322 181 10 27 345 24 325 3.07e-64 211
Msa1019270 MtrunA17_Chr4g0038941 36.420 324 187 8 27 349 53 358 6.10e-63 208
Msa1019270 MtrunA17_Chr4g0039011 34.412 340 201 9 14 350 30 350 1.91e-62 207
Msa1019270 MtrunA17_Chr7g0250301 34.906 318 195 6 27 344 9 314 3.26e-62 205
Msa1019270 MtrunA17_Chr4g0038961 36.810 326 183 10 27 349 24 329 5.36e-62 205
Msa1019270 MtrunA17_Chr4g0039071 34.891 321 197 6 27 346 26 335 9.47e-61 203
Msa1019270 MtrunA17_Chr4g0038971 35.890 326 186 10 27 349 24 329 4.27e-60 200
Msa1019270 MtrunA17_Chr1g0174261 38.095 294 168 7 55 346 1 282 5.57e-59 197
Msa1019270 MtrunA17_Chr2g0306311 29.184 490 287 17 2 453 9 476 6.34e-58 199
Msa1019270 MtrunA17_Chr8g0383311 32.028 434 270 16 27 452 44 460 1.00e-57 198
Msa1019270 MtrunA17_Chr1g0148391 30.909 440 266 17 27 452 27 442 2.51e-55 191
Msa1019270 MtrunA17_Chr4g0069801 31.382 427 259 18 38 452 39 443 3.16e-55 191
Msa1019270 MtrunA17_Chr4g0039021 36.644 292 167 8 53 341 8 284 3.17e-55 186
Msa1019270 MtrunA17_Chr4g0062771 29.374 463 293 19 4 453 10 451 1.60e-53 187
Msa1019270 MtrunA17_Chr2g0290821 31.364 440 276 16 21 452 23 444 4.12e-53 186
Msa1019270 MtrunA17_Chr3g0138741 30.217 460 285 17 3 452 15 448 4.71e-53 186
Msa1019270 MtrunA17_Chr7g0250321 35.206 267 164 4 27 293 29 286 1.44e-51 176
Msa1019270 MtrunA17_Chr3g0129341 30.415 434 277 16 27 453 35 450 5.61e-51 180
Msa1019270 MtrunA17_Chr5g0436361 36.275 306 183 8 47 349 58 354 1.49e-50 176
Msa1019270 MtrunA17_Chr4g0076581 30.979 439 274 16 24 452 31 450 1.08e-48 174
Msa1019270 MtrunA17_Chr3g0105981 28.176 433 279 18 20 443 21 430 8.26e-46 165
Msa1019270 MtrunA17_Chr6g0461601 26.667 450 296 18 27 460 27 458 1.03e-43 160
Msa1019270 MtrunA17_Chr7g0253091 25.957 470 311 20 6 459 6 454 2.14e-41 154
Msa1019270 MtrunA17_Chr2g0294581 33.721 172 103 4 23 190 73 237 8.43e-25 103
Msa1019270 MtrunA17_Chr5g0401301 23.419 427 264 10 51 454 24 410 9.98e-25 105
Msa1019270 MtrunA17_Chr8g0364811 47.561 82 43 0 129 210 2 83 1.08e-18 80.9
Msa1019270 MtrunA17_Chr5g0447651 42.553 94 51 1 360 453 19 109 1.04e-17 79.0
Msa1019270 MtrunA17_Chr1g0213651 48.193 83 42 1 370 452 272 353 1.80e-17 84.0
Msa1019270 MtrunA17_Chr4g0076141 41.975 81 47 0 374 454 13 93 6.88e-16 73.2
Msa1019270 MtrunA17_Chr8g0378401 44.578 83 45 1 370 452 28 109 1.81e-15 72.8
Msa1019270 MtrunA17_Chr8g0378381 44.578 83 45 1 370 452 28 109 4.53e-15 71.6
Msa1019270 MtrunA17_Chr4g0018771 40.206 97 51 2 250 346 86 175 4.59e-15 74.3
Msa1019270 MtrunA17_Chr4g0018861 40.196 102 54 2 245 346 79 173 5.78e-15 73.9
Msa1019270 MtrunA17_Chr4g0018861 35.227 88 57 0 5 92 1 88 5.20e-11 62.4
Msa1019270 MtrunA17_Chr4g0076131 39.241 79 48 0 374 452 28 106 9.22e-15 70.5
Msa1019270 MtrunA17_Chr8g0381081 40.426 94 55 1 360 452 42 135 1.76e-14 71.6
Msa1019270 MtrunA17_Chr3g0120401 42.045 88 50 1 365 452 148 234 5.65e-14 71.6
Msa1019270 MtrunA17_Chr3g0096451 38.235 102 61 2 352 452 89 189 2.79e-13 69.7
Msa1019270 MtrunA17_Chr8g0378371 44.444 81 41 2 372 452 31 107 7.18e-13 65.1
Msa1019270 MtrunA17_Chr8g0378361 43.038 79 44 1 374 452 30 107 1.08e-12 66.6
Msa1019270 MtrunA17_Chr8g0380231 41.667 72 41 1 389 460 32 102 2.42e-11 60.5
Msa1019270 MtrunA17_Chr5g0443331 39.759 83 49 1 370 452 104 185 4.19e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1019270 AT3G07320.1 66.667 435 143 1 18 452 17 449 0.0 617
Msa1019270 AT3G23770.1 55.300 434 190 2 21 451 32 464 0.0 522
Msa1019270 AT4G14080.1 52.034 467 207 2 1 451 1 466 0.0 521
Msa1019270 AT5G55180.2 42.188 448 240 7 21 460 22 458 1.21e-122 366
Msa1019270 AT4G26830.1 41.365 469 250 9 1 463 1 450 5.95e-122 364
Msa1019270 AT4G26830.2 42.529 435 232 7 23 451 26 448 7.51e-120 358
Msa1019270 AT5G55180.1 41.913 439 236 7 21 451 22 449 2.37e-118 355
Msa1019270 AT2G05790.1 39.318 440 248 7 27 451 26 461 1.00e-109 333
Msa1019270 AT5G56590.1 39.631 434 253 6 21 452 21 447 1.73e-107 328
Msa1019270 AT4G29360.2 38.689 473 258 7 4 452 15 479 1.62e-101 314
Msa1019270 AT4G29360.1 39.035 456 246 7 21 452 22 469 2.26e-101 314
Msa1019270 AT5G24318.2 37.665 454 272 7 1 452 5 449 4.08e-98 303
Msa1019270 AT5G24318.1 37.665 454 272 7 1 452 5 449 6.52e-98 302
Msa1019270 AT2G01630.3 35.897 429 258 7 27 452 24 438 2.76e-91 285
Msa1019270 AT4G34480.1 36.739 460 264 10 1 452 1 441 6.05e-91 286
Msa1019270 AT2G01630.1 35.814 430 259 7 27 453 24 439 1.44e-90 285
Msa1019270 AT2G16230.1 36.659 431 255 8 27 452 26 443 2.74e-89 281
Msa1019270 AT3G55430.1 36.842 437 254 12 21 452 24 443 3.92e-87 274
Msa1019270 AT3G15800.1 40.299 335 194 5 18 350 35 365 1.10e-85 269
Msa1019270 AT3G13560.4 33.256 433 263 7 27 452 27 440 1.05e-84 270
Msa1019270 AT3G13560.1 33.256 433 263 7 27 452 27 440 1.05e-84 270
Msa1019270 AT3G13560.2 33.256 433 263 7 27 452 27 440 1.05e-84 270
Msa1019270 AT3G13560.5 33.256 433 263 7 27 452 27 440 1.05e-84 270
Msa1019270 AT3G13560.3 33.256 433 263 7 27 452 27 440 1.05e-84 270
Msa1019270 AT1G66250.1 33.953 430 266 8 27 453 33 447 2.91e-84 268
Msa1019270 AT1G11820.3 33.179 431 271 7 27 454 45 461 8.06e-84 266
Msa1019270 AT4G18340.2 41.358 324 184 5 27 349 35 353 1.46e-83 263
Msa1019270 AT2G27500.2 38.906 329 196 4 27 354 30 354 2.80e-83 262
Msa1019270 AT4G18340.1 41.358 324 184 5 27 349 35 353 2.86e-83 263
Msa1019270 AT2G27500.1 38.906 329 196 4 27 354 30 354 8.86e-83 261
Msa1019270 AT1G30080.2 40.233 343 199 5 5 346 15 352 5.30e-82 259
Msa1019270 AT1G11820.2 32.791 430 272 7 27 453 45 460 7.09e-82 263
Msa1019270 AT1G30080.1 40.233 343 199 5 5 346 15 352 1.68e-81 258
Msa1019270 AT5G24318.4 40.000 350 200 6 1 348 5 346 1.79e-80 254
Msa1019270 AT1G32860.1 39.875 321 189 4 27 346 30 347 1.45e-79 254
Msa1019270 AT5G42720.1 40.227 353 200 7 2 352 7 350 7.38e-79 252
Msa1019270 AT2G26600.1 35.398 339 211 5 21 355 29 363 7.64e-75 240
Msa1019270 AT5G42100.2 39.024 328 193 6 24 348 25 348 1.12e-74 241
Msa1019270 AT3G57270.1 39.264 326 184 7 25 348 26 339 1.21e-74 238
Msa1019270 AT5G42100.1 39.024 328 193 6 24 348 25 348 1.61e-74 241
Msa1019270 AT4G34480.2 37.778 360 213 7 1 356 1 353 6.95e-74 237
Msa1019270 AT2G39640.1 35.320 453 265 14 4 452 8 436 2.49e-73 241
Msa1019270 AT3G61810.1 38.110 328 187 5 27 349 56 372 2.87e-72 233
Msa1019270 AT2G01630.2 35.561 374 224 7 27 397 24 383 4.46e-72 233
Msa1019270 AT4G16260.1 36.752 351 199 11 3 349 4 335 1.80e-67 220
Msa1019270 AT5G24318.3 39.145 304 177 4 149 452 15 310 3.62e-66 216
Msa1019270 AT4G34480.3 38.081 344 192 7 114 452 3 330 1.29e-65 216
Msa1019270 AT2G27500.3 38.351 279 167 4 77 354 2 276 5.80e-65 212
Msa1019270 AT3G46570.1 36.919 344 205 8 8 348 10 344 3.48e-64 212
Msa1019270 AT5G20390.1 37.202 336 197 9 4 338 12 334 8.73e-63 207
Msa1019270 AT1G33220.1 37.048 332 193 9 3 330 8 327 1.15e-62 207
Msa1019270 AT4G16260.2 36.562 320 187 9 33 350 2 307 2.10e-62 206
Msa1019270 AT5G20330.1 37.059 340 198 9 3 338 8 335 2.82e-62 206
Msa1019270 AT5G20340.1 37.855 317 184 8 27 342 44 348 4.33e-62 206
Msa1019270 AT3G57260.1 33.526 346 212 8 4 349 12 339 3.21e-61 203
Msa1019270 AT3G57260.2 33.815 346 211 8 4 349 28 355 5.72e-61 203
Msa1019270 AT2G26600.2 36.630 273 165 5 87 355 1 269 9.01e-60 198
Msa1019270 AT1G11820.1 32.722 327 213 5 27 350 45 367 2.48e-59 200
Msa1019270 AT1G77780.1 33.430 344 217 7 3 346 1 332 1.82e-58 197
Msa1019270 AT3G24330.1 30.197 457 294 16 5 452 18 458 2.99e-58 200
Msa1019270 AT3G57240.1 34.177 316 189 8 25 339 34 331 6.11e-56 189
Msa1019270 AT4G31140.1 28.211 436 282 17 27 452 28 442 5.49e-50 177
Msa1019270 AT3G55780.1 32.698 315 198 8 40 349 49 354 8.94e-50 174
Msa1019270 AT1G77790.1 31.562 320 210 5 27 346 31 341 1.37e-48 170
Msa1019270 AT5G20560.1 35.673 342 201 11 3 338 8 336 4.29e-48 169
Msa1019270 AT5G18220.1 28.761 452 287 18 16 453 20 450 4.07e-47 170
Msa1019270 AT4G17180.1 30.110 455 281 18 6 452 9 434 4.58e-47 169
Msa1019270 AT5G20870.1 29.231 455 288 19 32 471 37 472 6.55e-47 170
Msa1019270 AT3G04010.1 27.790 457 294 19 11 453 18 452 2.84e-44 162
Msa1019270 AT5G58090.1 28.864 440 284 17 23 452 19 439 2.17e-43 159
Msa1019270 AT5G64790.1 26.087 437 292 15 27 452 29 445 1.26e-41 155
Msa1019270 AT2G19440.2 27.918 437 286 19 27 453 22 439 4.73e-40 150
Msa1019270 AT2G19440.1 27.918 437 286 19 27 453 22 439 4.73e-40 150
Msa1019270 AT5G58480.1 27.045 440 281 16 27 453 27 439 2.40e-39 148
Msa1019270 AT1G64760.1 27.397 438 287 20 27 453 26 443 4.91e-39 147
Msa1019270 AT1G64760.2 27.397 438 287 20 27 453 26 443 4.91e-39 147
Msa1019270 AT5G67460.2 43.689 103 52 2 358 454 271 373 1.03e-19 91.3
Msa1019270 AT5G67460.1 43.564 101 51 2 358 452 271 371 4.82e-19 89.4
Msa1019270 AT5G35740.1 37.895 95 57 2 359 452 16 109 1.53e-14 70.5
Msa1019270 AT4G05430.2 38.235 102 58 2 353 453 30 127 2.21e-14 70.1
Msa1019270 AT4G05430.1 35.772 123 66 4 353 472 5 117 2.77e-14 70.5
Msa1019270 AT2G04910.2 39.241 79 48 0 374 452 15 93 5.27e-14 68.6
Msa1019270 AT1G79480.1 43.038 79 44 1 374 452 311 388 9.50e-14 73.2
Msa1019270 AT1G79480.2 43.038 79 44 1 374 452 310 387 9.98e-14 73.2
Msa1019270 AT2G04910.1 40.000 80 48 0 374 453 15 94 2.74e-13 67.0
Msa1019270 AT4G05430.3 35.772 123 66 4 353 472 30 142 6.58e-13 67.4
Msa1019270 AT2G29735.1 38.824 85 49 2 370 452 27 110 1.06e-12 65.1
Msa1019270 AT4G13600.1 45.000 80 43 1 373 452 60 138 1.79e-12 67.4
Msa1019270 AT4G09462.1 37.931 87 53 1 366 452 21 106 3.27e-12 63.5
Msa1019270 AT1G66870.2 35.366 82 53 0 371 452 50 131 4.20e-12 63.9
Msa1019270 AT1G66870.1 35.366 82 53 0 371 452 23 104 5.71e-12 62.8
Msa1019270 AT5G61130.1 40.506 79 46 1 374 452 21 98 6.53e-12 65.1
Msa1019270 AT1G29380.4 41.250 80 46 1 374 453 148 226 7.73e-12 65.5
Msa1019270 AT1G29380.2 41.250 80 46 1 374 453 148 226 7.73e-12 65.5
Msa1019270 AT1G29380.1 41.250 80 46 1 374 453 148 226 8.22e-12 66.6
Msa1019270 AT2G30933.2 43.038 79 44 1 374 452 82 159 9.45e-12 63.5
Msa1019270 AT2G30933.3 43.038 79 44 1 374 452 82 159 9.45e-12 63.5
Msa1019270 AT1G29380.3 41.250 80 46 1 374 453 148 226 1.30e-11 65.5
Msa1019270 AT2G30933.1 39.362 94 55 2 360 452 67 159 1.31e-11 64.7
Msa1019270 AT4G09465.1 38.272 81 49 1 372 452 29 108 2.69e-11 61.2
Msa1019270 AT4G09464.1 38.272 81 49 1 372 452 29 108 2.75e-11 61.2
Msa1019270 AT4G09467.1 38.272 81 49 1 372 452 29 108 2.75e-11 61.2
Msa1019270 AT5G63250.1 33.000 100 61 2 355 452 27 122 2.78e-11 61.6

Find 158 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGATGCGCTTTGCGTTTA+AGG 0.094987 7_1:-40708795 None:intergenic
CGACCCGGTTAATGTTAATT+TGG 0.198547 7_1:+40709273 Msa1019270:CDS
GGAGGAACTTGTTACTTTAA+TGG 0.203799 7_1:+40709952 Msa1019270:CDS
TATAAGAGGAAATTTAAATT+TGG 0.215220 7_1:+40710548 Msa1019270:three_prime_UTR
CACCAAATCTTCCCTTTATA+TGG 0.228008 7_1:-40709751 None:intergenic
GATGGAGAAACTTGGGTTTC+CGG 0.239471 7_1:+40709399 Msa1019270:CDS
GCTATGCGTTTAGTTCTTAT+TGG 0.266539 7_1:+40709911 Msa1019270:CDS
CTACGAGGAGGTAACGGATT+AGG 0.270443 7_1:-40708976 None:intergenic
GTTTATGAGATTGATCTTTC+GGG 0.289787 7_1:+40709676 Msa1019270:CDS
TAATCCAAATTAACATTAAC+CGG 0.304337 7_1:-40709277 None:intergenic
TTTAAGGTTGTTAGGATTTG+TGG 0.304363 7_1:-40708833 None:intergenic
AGCATCATGATTTCAAATAA+TGG 0.307100 7_1:-40710748 None:intergenic
GGTTTATGAGATTGATCTTT+CGG 0.309669 7_1:+40709675 Msa1019270:CDS
GAGAATAATGAGCCATATAA+AGG 0.315438 7_1:+40709739 Msa1019270:CDS
ACGGTTAAAGATCCGGGTTC+GGG 0.326064 7_1:+40709325 Msa1019270:CDS
GAGGAACTTGTTACTTTAAT+GGG 0.332834 7_1:+40709953 Msa1019270:CDS
TAAAGATCCGGGTTCGGGTT+TGG 0.346500 7_1:+40709330 Msa1019270:CDS
ATTAGGGTCTGTGGGTAATA+AGG 0.348258 7_1:-40708959 None:intergenic
TTAATTGACATTTCTATAAG+AGG 0.349394 7_1:+40710534 Msa1019270:three_prime_UTR
CGGATATTTATCGCGGAAAC+GGG 0.349889 7_1:+40709427 Msa1019270:CDS
TTTGGGTTGTTGTATCCAAA+TGG 0.358606 7_1:+40709646 Msa1019270:CDS
TTGCAACACCTGGATTCATT+TGG 0.358963 7_1:+40710830 Msa1019270:three_prime_UTR
CTATGCGTTTAGTTCTTATT+GGG 0.362480 7_1:+40709912 Msa1019270:CDS
TCCATTGGTCGGAGAGGGTT+TGG 0.366090 7_1:-40708919 None:intergenic
AATGTAAGTTCCTCCTTTGA+AGG 0.366921 7_1:-40710641 None:intergenic
AGCCATATAAAGGGAAGATT+TGG 0.372684 7_1:+40709749 Msa1019270:CDS
TGTTGGGACACCGGCTCGAC+CGG 0.385430 7_1:+40709549 Msa1019270:CDS
GCGGAAACGGGTTGGCCTAA+TGG 0.386035 7_1:+40709439 Msa1019270:CDS
TTGGGAGCATGATTGAGATT+TGG 0.386715 7_1:-40708868 None:intergenic
AGTTGACCGTAGCAGATTCC+TGG 0.387463 7_1:-40708725 None:intergenic
TGCGTTTAAGGATTTGATTA+AGG 0.388788 7_1:-40708783 None:intergenic
TTAAGGTTGTTAGGATTTGT+GGG 0.390167 7_1:-40708832 None:intergenic
AGATGAAGCCACGTGGAGTT+TGG 0.393557 7_1:-40709022 None:intergenic
AACTTGGGTTTCCGGATGTT+CGG 0.396533 7_1:+40709407 Msa1019270:CDS
TACGAGGAGGTAACGGATTA+GGG 0.400881 7_1:-40708975 None:intergenic
GTTTAATGAGAATATTAAAC+CGG 0.401130 7_1:+40709603 Msa1019270:CDS
TCATAAACCCTCGACCCATT+TGG 0.405372 7_1:-40709661 None:intergenic
AGATGGATGGATCCTGGAAA+TGG 0.405663 7_1:-40708325 None:intergenic
GGTGGTGATGTTGATCAAAT+TGG 0.406359 7_1:+40709460 Msa1019270:CDS
TACGGTTAAAGATCCGGGTT+CGG 0.410510 7_1:+40709324 Msa1019270:CDS
ACACAACCCAACCCATTTCC+AGG 0.413447 7_1:+40708313 Msa1019270:five_prime_UTR
AGTGCTGGAAACTCCGACTC+TGG 0.417051 7_1:-40709706 None:intergenic
TGAGAATATTAAACCGGGTC+CGG 0.418152 7_1:+40709609 Msa1019270:CDS
TCGGATATTTATCGCGGAAA+CGG 0.419442 7_1:+40709426 Msa1019270:CDS
TAATGTTACGGTTAAAGATC+CGG 0.423841 7_1:+40709318 Msa1019270:CDS
ATGCTTGCTCGCAAGGGAAC+GGG 0.427594 7_1:+40709824 Msa1019270:CDS
ATGTTTGTGATGAGTTCGTT+TGG 0.429565 7_1:-40708887 None:intergenic
GGGAGTAATGTGACGGCACT+AGG 0.433307 7_1:+40709787 Msa1019270:CDS
TGAAATGTTGTTGAGTAGAA+TGG 0.438583 7_1:-40708545 None:intergenic
TACGAATTTATTTGATCAGA+TGG 0.440268 7_1:+40709357 Msa1019270:CDS
AGTTGGTACTTCTTCTGCTA+TGG 0.450725 7_1:+40709105 Msa1019270:CDS
TGGGCACAGTTTAAGAAGAC+TGG 0.452877 7_1:+40709931 Msa1019270:CDS
TTACGAAACGCAGCGTTTGA+AGG 0.455167 7_1:-40709151 None:intergenic
AAGTTATTAAACCGATGCTT+AGG 0.456379 7_1:+40709188 Msa1019270:CDS
TTCTCCGGTGGAGGAAGTGC+TGG 0.459187 7_1:-40709721 None:intergenic
GGGAAGATAAAAGATAAAGT+AGG 0.468702 7_1:-40708348 None:intergenic
AGAATAATGAGCCATATAAA+GGG 0.469941 7_1:+40709740 Msa1019270:CDS
TGTTTGTGATGAGTTCGTTT+GGG 0.471998 7_1:-40708886 None:intergenic
ACACCTGGATTCATTTGGAT+TGG 0.472515 7_1:+40710835 Msa1019270:three_prime_UTR
AGAAGATTGAGATGAGACTA+TGG 0.473878 7_1:-40708285 None:intergenic
TTGGGTTGTTGTATCCAAAT+GGG 0.473881 7_1:+40709647 Msa1019270:CDS
CGAGCCGGTGTCCCAACAAC+CGG 0.475288 7_1:-40709544 None:intergenic
TATTAAACCGGGTCCGGGTA+CGG 0.476797 7_1:+40709615 Msa1019270:CDS
GAAAGTATTTGTAAATAAAG+AGG 0.477496 7_1:-40708157 None:intergenic
AGTAGGAGATGGATGGATCC+TGG 0.478674 7_1:-40708331 None:intergenic
TTGTCGTATGCTTGCTCGCA+AGG 0.480812 7_1:+40709817 Msa1019270:CDS
TTTCCCTTGGAGCTCCGACC+CGG 0.482089 7_1:+40709258 Msa1019270:CDS
GAATGTTGGATTTGATCCAT+TGG 0.484368 7_1:-40708934 None:intergenic
CAATTAATGAATGATTCAAA+TGG 0.485467 7_1:-40710518 None:intergenic
CCAAAATGCCGCTCCGTACC+CGG 0.485733 7_1:-40709628 None:intergenic
TTCCAAGTGTGACACTTTGA+AGG 0.487564 7_1:+40710477 Msa1019270:CDS
ACTATGGTGGTTTGATAAAG+TGG 0.487735 7_1:-40708269 None:intergenic
CGTTTCCTACGAGGAGGTAA+CGG 0.487887 7_1:-40708982 None:intergenic
CTCCTTCAAAGTGTCACACT+TGG 0.488468 7_1:-40710479 None:intergenic
GTTTAGAGAGAGTAATGTTA+CGG 0.488551 7_1:+40709306 Msa1019270:CDS
ATAAAAGATAAAGTAGGAGA+TGG 0.491479 7_1:-40708342 None:intergenic
TTATATGGCTCATTATTCTC+CGG 0.491934 7_1:-40709736 None:intergenic
TGTTGTATCCAAATGGGTCG+AGG 0.492149 7_1:+40709653 Msa1019270:CDS
TGATCAACATCACCACCATT+AGG 0.494772 7_1:-40709454 None:intergenic
TATTCATTTATTGCAACACC+TGG 0.497950 7_1:+40710820 Msa1019270:three_prime_UTR
GTAGAATGGAGAAGAGAAAG+AGG 0.501465 7_1:-40708531 None:intergenic
CGAAACGCAGCGTTTGAAGG+TGG 0.507919 7_1:-40709148 None:intergenic
TTTGATCCATTGGTCGGAGA+GGG 0.508250 7_1:-40708924 None:intergenic
ATTTGGTGTGTGGCGGTGCG+TGG 0.510143 7_1:+40709766 Msa1019270:CDS
ATGGATCCTGGAAATGGGTT+GGG 0.510825 7_1:-40708319 None:intergenic
GAGAATATTAAACCGGGTCC+GGG 0.512487 7_1:+40709610 Msa1019270:CDS
TGTTTAATTCTACGCATCGC+AGG 0.512620 7_1:-40709049 None:intergenic
TGGGTAATAAGGTAGAATGT+TGG 0.512790 7_1:-40708948 None:intergenic
CTTTCAGAAGTTGTGCTATG+TGG 0.515432 7_1:-40708466 None:intergenic
TGCCGCTCCGTACCCGGACC+CGG 0.517826 7_1:-40709622 None:intergenic
GCGATAAATATCCGAACATC+CGG 0.517870 7_1:-40709418 None:intergenic
GATGGATGGATCCTGGAAAT+GGG 0.518644 7_1:-40708324 None:intergenic
AATGTTACGGTTAAAGATCC+GGG 0.527447 7_1:+40709319 Msa1019270:CDS
ATTTGATCAGATGGTGGATG+CGG 0.528077 7_1:+40709366 Msa1019270:CDS
GTTGGGACACCGGCTCGACC+GGG 0.530784 7_1:+40709550 Msa1019270:CDS
GTTTCCAGCACTTCCTCCAC+CGG 0.531728 7_1:+40709717 Msa1019270:CDS
GTTTAATTCTACGCATCGCA+GGG 0.533547 7_1:-40709048 None:intergenic
GGCTCATTATTCTCCGGTGG+AGG 0.534146 7_1:-40709730 None:intergenic
GGAATCTGCTACGGTCAACT+AGG 0.534218 7_1:+40708728 Msa1019270:CDS
AGAATCAAACCCGGTCGAGC+CGG 0.535734 7_1:-40709559 None:intergenic
AGAATCAAAAGAATCAAAGT+TGG 0.542291 7_1:+40709088 Msa1019270:CDS
GATGGATCCTGGAAATGGGT+TGG 0.544148 7_1:-40708320 None:intergenic
GAAGGTTGAATGTTACTAGA+GGG 0.544294 7_1:-40710623 None:intergenic
TAGTAACATTCAACCTTCAA+AGG 0.544730 7_1:+40710628 Msa1019270:three_prime_UTR
TATTTATCGCGGAAACGGGT+TGG 0.547640 7_1:+40709431 Msa1019270:CDS
CTATGGTGGTTTGATAAAGT+GGG 0.549418 7_1:-40708268 None:intergenic
TATGCTTGCTCGCAAGGGAA+CGG 0.549988 7_1:+40709823 Msa1019270:CDS
AAGATAAAGTAGGAGATGGA+TGG 0.552956 7_1:-40708338 None:intergenic
GCTTTCATTTCATCAATGTG+AGG 0.554721 7_1:+40710596 Msa1019270:three_prime_UTR
GGAGTAATGTGACGGCACTA+GGG 0.555095 7_1:+40709788 Msa1019270:CDS
ACCAAACCCTCTCCGACCAA+TGG 0.556784 7_1:+40708918 Msa1019270:CDS
GTTGGATTTGATCCATTGGT+CGG 0.560158 7_1:-40708930 None:intergenic
ACTTGTGACCCGATTCAACC+CGG 0.562733 7_1:+40709847 Msa1019270:CDS
TTTGGTGTGTGGCGGTGCGT+GGG 0.564836 7_1:+40709767 Msa1019270:CDS
AAAACCGGTTGTTGGGACAC+CGG 0.566975 7_1:+40709540 Msa1019270:CDS
CAAGTGTGACACTTTGAAGG+AGG 0.572437 7_1:+40710480 Msa1019270:CDS
GTATAAACCAAACCCGAACC+CGG 0.574382 7_1:-40709337 None:intergenic
AAGGTTGAATGTTACTAGAG+GGG 0.577100 7_1:-40710622 None:intergenic
GTTGTATCCAAATGGGTCGA+GGG 0.579411 7_1:+40709654 Msa1019270:CDS
GAATTTATTTGATCAGATGG+TGG 0.583307 7_1:+40709360 Msa1019270:CDS
TAGCCAATCCAAATGAATCC+AGG 0.590664 7_1:-40710838 None:intergenic
TTAATGAATGATTCAAATGG+AGG 0.591003 7_1:-40710515 None:intergenic
TAACCGGGTCGGAGCTCCAA+GGG 0.592039 7_1:-40709261 None:intergenic
GAGTAATGTGACGGCACTAG+GGG 0.602111 7_1:+40709789 Msa1019270:CDS
AGGTTGAATGTTACTAGAGG+GGG 0.602309 7_1:-40710621 None:intergenic
AGGTAACGGATTAGGGTCTG+TGG 0.612266 7_1:-40708968 None:intergenic
TGTCGTATGCTTGCTCGCAA+GGG 0.616038 7_1:+40709818 Msa1019270:CDS
ATTTGATCCATTGGTCGGAG+AGG 0.616370 7_1:-40708925 None:intergenic
TATGGCTCATTATTCTCCGG+TGG 0.617117 7_1:-40709733 None:intergenic
TGATTTCGTTTCCTACGAGG+AGG 0.619686 7_1:-40708988 None:intergenic
TTCGGGGAAGACACCAGAGT+CGG 0.620147 7_1:+40709693 Msa1019270:CDS
TTAACCGGGTCGGAGCTCCA+AGG 0.620353 7_1:-40709262 None:intergenic
CTTGTGACCCGATTCAACCC+GGG 0.621770 7_1:+40709848 Msa1019270:CDS
TAATGTGACGGCACTAGGGG+AGG 0.621819 7_1:+40709792 Msa1019270:CDS
AACCGGGTCCGGGTACGGAG+CGG 0.622333 7_1:+40709620 Msa1019270:CDS
CAAATTAACATTAACCGGGT+CGG 0.622515 7_1:-40709272 None:intergenic
TTTAATGAGAATATTAAACC+GGG 0.624692 7_1:+40709604 Msa1019270:CDS
TGAAGGTTGAATGTTACTAG+AGG 0.624775 7_1:-40710624 None:intergenic
TCACAACCAGGAATCTGCTA+CGG 0.630316 7_1:+40708719 Msa1019270:CDS
TAAAGGGAAGATTTGGTGTG+TGG 0.635044 7_1:+40709756 Msa1019270:CDS
GAAACGGGTTGGCCTAATGG+TGG 0.635140 7_1:+40709442 Msa1019270:CDS
GCTTGCTACTCAAACTGCAA+AGG 0.636317 7_1:+40709975 Msa1019270:CDS
AGATTGAGATGAGACTATGG+TGG 0.639702 7_1:-40708282 None:intergenic
CTAATCCGTTACCTCCTCGT+AGG 0.641103 7_1:+40708977 Msa1019270:CDS
GGTAACGGATTAGGGTCTGT+GGG 0.641342 7_1:-40708967 None:intergenic
TAACATTCAACCTTCAAAGG+AGG 0.643417 7_1:+40710631 Msa1019270:three_prime_UTR
AAGCCACGTGGAGTTTGGTG+TGG 0.644687 7_1:-40709017 None:intergenic
AGATGATTTCGTTTCCTACG+AGG 0.653727 7_1:-40708991 None:intergenic
TGCGTTTCGTAAAGATATAG+CGG 0.657744 7_1:+40709162 Msa1019270:CDS
AATCCAAATTAACATTAACC+GGG 0.666875 7_1:-40709276 None:intergenic
GGATGTTCGGATATTTATCG+CGG 0.670356 7_1:+40709420 Msa1019270:CDS
TTTATGAGATTGATCTTTCG+GGG 0.672089 7_1:+40709677 Msa1019270:CDS
CATCCACACCAAACTCCACG+TGG 0.678852 7_1:+40709014 Msa1019270:CDS
GCACAGTTTAAGAAGACTGG+AGG 0.681953 7_1:+40709934 Msa1019270:CDS
GGTGCGTGGGAGTAATGTGA+CGG 0.690661 7_1:+40709780 Msa1019270:CDS
AGGGAAGATTTGGTGTGTGG+CGG 0.713466 7_1:+40709759 Msa1019270:CDS
AAACTCGGAAGAATCAAACC+CGG 0.738088 7_1:-40709568 None:intergenic
AGGGACGAGATGAAGCCACG+TGG 0.766058 7_1:-40709029 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAGTTGTTTATTTTTTT+GGG - chr7_1:40708371-40708390 None:intergenic 10.0%
!!! TAATTGACTCTTTTTTTAAT+TGG - chr7_1:40708190-40708209 None:intergenic 15.0%
!!! GAAAAAGTTGTTTATTTTTT+TGG - chr7_1:40708372-40708391 None:intergenic 15.0%
!! TAAAAAAGTTATGAAAAAAC+CGG + chr7_1:40709525-40709544 Msa1019270:CDS 15.0%
!! AATAAAAAAATGTACTTACT+TGG - chr7_1:40710003-40710022 None:intergenic 15.0%
!!! TTTTCATTTTGATATAAGAA+AGG + chr7_1:40710122-40710141 Msa1019270:intron 15.0%
!! AAAATAAAAACGTTAAGTAA+TGG - chr7_1:40710417-40710436 None:intergenic 15.0%
!! TATAAGAGGAAATTTAAATT+TGG + chr7_1:40710548-40710567 Msa1019270:three_prime_UTR 15.0%
!!! GAAAGTATTTGTAAATAAAG+AGG - chr7_1:40708160-40708179 None:intergenic 20.0%
!! AAAAACAGCAAAAACAAAAA+TGG + chr7_1:40708498-40708517 Msa1019270:exon 20.0%
!! TTCTTATTAATATACTTCAC+AGG + chr7_1:40708680-40708699 Msa1019270:intron 20.0%
!! AAAAGAAAAAAGATTTAGTC+CGG - chr7_1:40709222-40709241 None:intergenic 20.0%
!!! GACTAAATCTTTTTTCTTTT+TGG + chr7_1:40709222-40709241 Msa1019270:CDS 20.0%
!! TAATCCAAATTAACATTAAC+CGG - chr7_1:40709280-40709299 None:intergenic 20.0%
!! AACAAAGCAAATAAAAAACT+CGG - chr7_1:40709586-40709605 None:intergenic 20.0%
!! GTTTAATGAGAATATTAAAC+CGG + chr7_1:40709603-40709622 Msa1019270:CDS 20.0%
!! TTTAATGAGAATATTAAACC+GGG + chr7_1:40709604-40709623 Msa1019270:CDS 20.0%
!!! GTTTTACAACAAAAAATTGT+TGG + chr7_1:40710148-40710167 Msa1019270:intron 20.0%
!! CAATTTAATGGTTATACATT+TGG - chr7_1:40710262-40710281 None:intergenic 20.0%
!! AATCAAGTACTTCAATTTAA+TGG - chr7_1:40710274-40710293 None:intergenic 20.0%
!! GTAGTTAAGTTTAAATTAAC+TGG - chr7_1:40710315-40710334 None:intergenic 20.0%
!!! AGTAATGGAAAAGTATAATA+AGG - chr7_1:40710402-40710421 None:intergenic 20.0%
!! CAATTAATGAATGATTCAAA+TGG - chr7_1:40710521-40710540 None:intergenic 20.0%
!! TTAATTGACATTTCTATAAG+AGG + chr7_1:40710534-40710553 Msa1019270:three_prime_UTR 20.0%
! ATAAAAGATAAAGTAGGAGA+TGG - chr7_1:40708345-40708364 None:intergenic 25.0%
!! CTACTTTCTATTTTCAATGT+TGG - chr7_1:40708422-40708441 None:intergenic 25.0%
!!! TTGTGTTTTTTAAGGTTGTT+AGG - chr7_1:40708844-40708863 None:intergenic 25.0%
!!! TTGGATGTTTGTGTTTTTTA+AGG - chr7_1:40708852-40708871 None:intergenic 25.0%
! AGAATCAAAAGAATCAAAGT+TGG + chr7_1:40709088-40709107 Msa1019270:CDS 25.0%
! AATCCAAATTAACATTAACC+GGG - chr7_1:40709279-40709298 None:intergenic 25.0%
! TACGAATTTATTTGATCAGA+TGG + chr7_1:40709357-40709376 Msa1019270:CDS 25.0%
! AGAATAATGAGCCATATAAA+GGG + chr7_1:40709740-40709759 Msa1019270:CDS 25.0%
! AATCATAACTCACAATTAGA+GGG - chr7_1:40710054-40710073 None:intergenic 25.0%
! TAATCATAACTCACAATTAG+AGG - chr7_1:40710055-40710074 None:intergenic 25.0%
!!! AAGTACTTGATTTGTTAACA+AGG + chr7_1:40710282-40710301 Msa1019270:intron 25.0%
! TTAATGAATGATTCAAATGG+AGG - chr7_1:40710518-40710537 None:intergenic 25.0%
!! ATGAATGATTTTCATAGCAA+TGG + chr7_1:40710665-40710684 Msa1019270:three_prime_UTR 25.0%
! AGCATCATGATTTCAAATAA+TGG - chr7_1:40710751-40710770 None:intergenic 25.0%
!! ATGCAACGACTATATTTTGA+AGG - chr7_1:40708215-40708234 None:intergenic 30.0%
GGGAAGATAAAAGATAAAGT+AGG - chr7_1:40708351-40708370 None:intergenic 30.0%
!!! CTATTTTCAATGTTGGTGAT+GGG - chr7_1:40708415-40708434 None:intergenic 30.0%
!! TCTATTTTCAATGTTGGTGA+TGG - chr7_1:40708416-40708435 None:intergenic 30.0%
TGAAATGTTGTTGAGTAGAA+TGG - chr7_1:40708548-40708567 None:intergenic 30.0%
! ATTAAGGAGAGTGATTTTGA+GGG - chr7_1:40708770-40708789 None:intergenic 30.0%
TGCGTTTAAGGATTTGATTA+AGG - chr7_1:40708786-40708805 None:intergenic 30.0%
TTAAGGTTGTTAGGATTTGT+GGG - chr7_1:40708835-40708854 None:intergenic 30.0%
! TTTAAGGTTGTTAGGATTTG+TGG - chr7_1:40708836-40708855 None:intergenic 30.0%
AAGTTATTAAACCGATGCTT+AGG + chr7_1:40709188-40709207 Msa1019270:CDS 30.0%
GTTTAGAGAGAGTAATGTTA+CGG + chr7_1:40709306-40709325 Msa1019270:CDS 30.0%
TAATGTTACGGTTAAAGATC+CGG + chr7_1:40709318-40709337 Msa1019270:CDS 30.0%
GAATTTATTTGATCAGATGG+TGG + chr7_1:40709360-40709379 Msa1019270:CDS 30.0%
TTATGAAAAAACCGGTTGTT+GGG + chr7_1:40709533-40709552 Msa1019270:CDS 30.0%
GGTTTATGAGATTGATCTTT+CGG + chr7_1:40709675-40709694 Msa1019270:CDS 30.0%
GTTTATGAGATTGATCTTTC+GGG + chr7_1:40709676-40709695 Msa1019270:CDS 30.0%
TTTATGAGATTGATCTTTCG+GGG + chr7_1:40709677-40709696 Msa1019270:CDS 30.0%
TTATATGGCTCATTATTCTC+CGG - chr7_1:40709739-40709758 None:intergenic 30.0%
GAGAATAATGAGCCATATAA+AGG + chr7_1:40709739-40709758 Msa1019270:CDS 30.0%
!! CTATGCGTTTAGTTCTTATT+GGG + chr7_1:40709912-40709931 Msa1019270:CDS 30.0%
!! GAGGAACTTGTTACTTTAAT+GGG + chr7_1:40709953-40709972 Msa1019270:CDS 30.0%
TGACATTTAACATCCATTGT+GGG - chr7_1:40710194-40710213 None:intergenic 30.0%
ATGACATTTAACATCCATTG+TGG - chr7_1:40710195-40710214 None:intergenic 30.0%
!!! ATCTTTTTTCGAACATGTGT+AGG + chr7_1:40710437-40710456 Msa1019270:intron 30.0%
TAGTAACATTCAACCTTCAA+AGG + chr7_1:40710628-40710647 Msa1019270:three_prime_UTR 30.0%
! ATGATTTTCATAGCAATGGT+AGG + chr7_1:40710669-40710688 Msa1019270:three_prime_UTR 30.0%
! TGATTTTCATAGCAATGGTA+GGG + chr7_1:40710670-40710689 Msa1019270:three_prime_UTR 30.0%
TATTCATTTATTGCAACACC+TGG + chr7_1:40710820-40710839 Msa1019270:three_prime_UTR 30.0%
CTATGGTGGTTTGATAAAGT+GGG - chr7_1:40708271-40708290 None:intergenic 35.0%
ACTATGGTGGTTTGATAAAG+TGG - chr7_1:40708272-40708291 None:intergenic 35.0%
AGAAGATTGAGATGAGACTA+TGG - chr7_1:40708288-40708307 None:intergenic 35.0%
AAGATAAAGTAGGAGATGGA+TGG - chr7_1:40708341-40708360 None:intergenic 35.0%
TTTCTTCAATCTCATCTCTC+TGG + chr7_1:40708592-40708611 Msa1019270:intron 35.0%
TTCTTCAATCTCATCTCTCT+GGG + chr7_1:40708593-40708612 Msa1019270:intron 35.0%
! GATTAAGGAGAGTGATTTTG+AGG - chr7_1:40708771-40708790 None:intergenic 35.0%
TGTTTGTGATGAGTTCGTTT+GGG - chr7_1:40708889-40708908 None:intergenic 35.0%
ATGTTTGTGATGAGTTCGTT+TGG - chr7_1:40708890-40708909 None:intergenic 35.0%
GAATGTTGGATTTGATCCAT+TGG - chr7_1:40708937-40708956 None:intergenic 35.0%
TGGGTAATAAGGTAGAATGT+TGG - chr7_1:40708951-40708970 None:intergenic 35.0%
TGCGTTTCGTAAAGATATAG+CGG + chr7_1:40709162-40709181 Msa1019270:CDS 35.0%
CGGTTTAAAAACCTAAGCAT+CGG - chr7_1:40709202-40709221 None:intergenic 35.0%
! CGATGCTTAGGTTTTTAAAC+CGG + chr7_1:40709200-40709219 Msa1019270:CDS 35.0%
!! ATGTTTATCCGTTTTTCCCT+TGG + chr7_1:40709245-40709264 Msa1019270:CDS 35.0%
CAAATTAACATTAACCGGGT+CGG - chr7_1:40709275-40709294 None:intergenic 35.0%
AATGTTACGGTTAAAGATCC+GGG + chr7_1:40709319-40709338 Msa1019270:CDS 35.0%
! TATTTTGCGATGGAGAAACT+TGG + chr7_1:40709391-40709410 Msa1019270:CDS 35.0%
! ATTTTGCGATGGAGAAACTT+GGG + chr7_1:40709392-40709411 Msa1019270:CDS 35.0%
GTTATGAAAAAACCGGTTGT+TGG + chr7_1:40709532-40709551 Msa1019270:CDS 35.0%
!! TTTGGGTTGTTGTATCCAAA+TGG + chr7_1:40709646-40709665 Msa1019270:CDS 35.0%
! TTGGGTTGTTGTATCCAAAT+GGG + chr7_1:40709647-40709666 Msa1019270:CDS 35.0%
AGCCATATAAAGGGAAGATT+TGG + chr7_1:40709749-40709768 Msa1019270:CDS 35.0%
CACCAAATCTTCCCTTTATA+TGG - chr7_1:40709754-40709773 None:intergenic 35.0%
!! AAAGTGTTTTAAGCCCGATT+CGG + chr7_1:40709873-40709892 Msa1019270:CDS 35.0%
!! GCTATGCGTTTAGTTCTTAT+TGG + chr7_1:40709911-40709930 Msa1019270:CDS 35.0%
!! GGAGGAACTTGTTACTTTAA+TGG + chr7_1:40709952-40709971 Msa1019270:CDS 35.0%
TCTTAGTATTGTTCCCACAA+TGG + chr7_1:40710178-40710197 Msa1019270:intron 35.0%
TTTCGAACATGTGTAGGTTA+TGG + chr7_1:40710443-40710462 Msa1019270:intron 35.0%
GCTTTCATTTCATCAATGTG+AGG + chr7_1:40710596-40710615 Msa1019270:three_prime_UTR 35.0%
AAGGTTGAATGTTACTAGAG+GGG - chr7_1:40710625-40710644 None:intergenic 35.0%
GAAGGTTGAATGTTACTAGA+GGG - chr7_1:40710626-40710645 None:intergenic 35.0%
TGAAGGTTGAATGTTACTAG+AGG - chr7_1:40710627-40710646 None:intergenic 35.0%
TAACATTCAACCTTCAAAGG+AGG + chr7_1:40710631-40710650 Msa1019270:three_prime_UTR 35.0%
AATGTAAGTTCCTCCTTTGA+AGG - chr7_1:40710644-40710663 None:intergenic 35.0%
AGATTGAGATGAGACTATGG+TGG - chr7_1:40708285-40708304 None:intergenic 40.0%
CTTTCAGAAGTTGTGCTATG+TGG - chr7_1:40708469-40708488 None:intergenic 40.0%
GTAGAATGGAGAAGAGAAAG+AGG - chr7_1:40708534-40708553 None:intergenic 40.0%
!!! TCAGCTTTTTCATCACAACC+AGG + chr7_1:40708707-40708726 Msa1019270:CDS 40.0%
!! GTGATTTTGAGGGTTTTGGT+AGG - chr7_1:40708760-40708779 None:intergenic 40.0%
!! GAGAGTGATTTTGAGGGTTT+TGG - chr7_1:40708764-40708783 None:intergenic 40.0%
TTTGATGCGCTTTGCGTTTA+AGG - chr7_1:40708798-40708817 None:intergenic 40.0%
! TTGGGAGCATGATTGAGATT+TGG - chr7_1:40708871-40708890 None:intergenic 40.0%
GTTGGATTTGATCCATTGGT+CGG - chr7_1:40708933-40708952 None:intergenic 40.0%
ATTAGGGTCTGTGGGTAATA+AGG - chr7_1:40708962-40708981 None:intergenic 40.0%
AGATGATTTCGTTTCCTACG+AGG - chr7_1:40708994-40709013 None:intergenic 40.0%
GTTTAATTCTACGCATCGCA+GGG - chr7_1:40709051-40709070 None:intergenic 40.0%
TGTTTAATTCTACGCATCGC+AGG - chr7_1:40709052-40709071 None:intergenic 40.0%
! AGTTGGTACTTCTTCTGCTA+TGG + chr7_1:40709105-40709124 Msa1019270:CDS 40.0%
CGACCCGGTTAATGTTAATT+TGG + chr7_1:40709273-40709292 Msa1019270:CDS 40.0%
!! ATTTGATCAGATGGTGGATG+CGG + chr7_1:40709366-40709385 Msa1019270:CDS 40.0%
GCGATAAATATCCGAACATC+CGG - chr7_1:40709421-40709440 None:intergenic 40.0%
GGATGTTCGGATATTTATCG+CGG + chr7_1:40709420-40709439 Msa1019270:CDS 40.0%
TCGGATATTTATCGCGGAAA+CGG + chr7_1:40709426-40709445 Msa1019270:CDS 40.0%
TGATCAACATCACCACCATT+AGG - chr7_1:40709457-40709476 None:intergenic 40.0%
! GGTGGTGATGTTGATCAAAT+TGG + chr7_1:40709460-40709479 Msa1019270:CDS 40.0%
AAACTCGGAAGAATCAAACC+CGG - chr7_1:40709571-40709590 None:intergenic 40.0%
TGAGAATATTAAACCGGGTC+CGG + chr7_1:40709609-40709628 Msa1019270:CDS 40.0%
!! TAAAGGGAAGATTTGGTGTG+TGG + chr7_1:40709756-40709775 Msa1019270:CDS 40.0%
!!! GGCTTAAAACACTTTTTCCC+GGG - chr7_1:40709868-40709887 None:intergenic 40.0%
!!! GGGCTTAAAACACTTTTTCC+CGG - chr7_1:40709869-40709888 None:intergenic 40.0%
!!! TTAAGCCCGATTCGGATTTT+TGG + chr7_1:40709881-40709900 Msa1019270:CDS 40.0%
AGCATGCCAAAAATCCGAAT+CGG - chr7_1:40709890-40709909 None:intergenic 40.0%
TTCCAAGTGTGACACTTTGA+AGG + chr7_1:40710477-40710496 Msa1019270:CDS 40.0%
AGGTTGAATGTTACTAGAGG+GGG - chr7_1:40710624-40710643 None:intergenic 40.0%
TTGCAACACCTGGATTCATT+TGG + chr7_1:40710830-40710849 Msa1019270:three_prime_UTR 40.0%
! ATGGATCCTGGAAATGGGTT+GGG - chr7_1:40708322-40708341 None:intergenic 45.0%
GATGGATGGATCCTGGAAAT+GGG - chr7_1:40708327-40708346 None:intergenic 45.0%
AGATGGATGGATCCTGGAAA+TGG - chr7_1:40708328-40708347 None:intergenic 45.0%
TCACAACCAGGAATCTGCTA+CGG + chr7_1:40708719-40708738 Msa1019270:CDS 45.0%
TTTGATCCATTGGTCGGAGA+GGG - chr7_1:40708927-40708946 None:intergenic 45.0%
ATTTGATCCATTGGTCGGAG+AGG - chr7_1:40708928-40708947 None:intergenic 45.0%
!! TACGAGGAGGTAACGGATTA+GGG - chr7_1:40708978-40708997 None:intergenic 45.0%
TGATTTCGTTTCCTACGAGG+AGG - chr7_1:40708991-40709010 None:intergenic 45.0%
TTACGAAACGCAGCGTTTGA+AGG - chr7_1:40709154-40709173 None:intergenic 45.0%
TACGGTTAAAGATCCGGGTT+CGG + chr7_1:40709324-40709343 Msa1019270:CDS 45.0%
GTATAAACCAAACCCGAACC+CGG - chr7_1:40709340-40709359 None:intergenic 45.0%
!!! GGATGCGGTTTATTTTGCGA+TGG + chr7_1:40709381-40709400 Msa1019270:CDS 45.0%
GATGGAGAAACTTGGGTTTC+CGG + chr7_1:40709399-40709418 Msa1019270:CDS 45.0%
AACTTGGGTTTCCGGATGTT+CGG + chr7_1:40709407-40709426 Msa1019270:CDS 45.0%
CGGATATTTATCGCGGAAAC+GGG + chr7_1:40709427-40709446 Msa1019270:CDS 45.0%
TATTTATCGCGGAAACGGGT+TGG + chr7_1:40709431-40709450 Msa1019270:CDS 45.0%
GAGAATATTAAACCGGGTCC+GGG + chr7_1:40709610-40709629 Msa1019270:CDS 45.0%
TGTTGTATCCAAATGGGTCG+AGG + chr7_1:40709653-40709672 Msa1019270:CDS 45.0%
GTTGTATCCAAATGGGTCGA+GGG + chr7_1:40709654-40709673 Msa1019270:CDS 45.0%
TCATAAACCCTCGACCCATT+TGG - chr7_1:40709664-40709683 None:intergenic 45.0%
TATGGCTCATTATTCTCCGG+TGG - chr7_1:40709736-40709755 None:intergenic 45.0%
!! ACTTTTTCCCGGGTTGAATC+GGG - chr7_1:40709858-40709877 None:intergenic 45.0%
!!! CACTTTTTCCCGGGTTGAAT+CGG - chr7_1:40709859-40709878 None:intergenic 45.0%
GCATGCCAAAAATCCGAATC+GGG - chr7_1:40709889-40709908 None:intergenic 45.0%
TGGGCACAGTTTAAGAAGAC+TGG + chr7_1:40709931-40709950 Msa1019270:CDS 45.0%
GCACAGTTTAAGAAGACTGG+AGG + chr7_1:40709934-40709953 Msa1019270:CDS 45.0%
! GCTTGCTACTCAAACTGCAA+AGG + chr7_1:40709975-40709994 Msa1019270:CDS 45.0%
! CTCCTTCAAAGTGTCACACT+TGG - chr7_1:40710482-40710501 None:intergenic 45.0%
! CAAGTGTGACACTTTGAAGG+AGG + chr7_1:40710480-40710499 Msa1019270:CDS 45.0%
ACACAACCCAACCCATTTCC+AGG + chr7_1:40708313-40708332 Msa1019270:five_prime_UTR 50.0%
GATGGATCCTGGAAATGGGT+TGG - chr7_1:40708323-40708342 None:intergenic 50.0%
AGTAGGAGATGGATGGATCC+TGG - chr7_1:40708334-40708353 None:intergenic 50.0%
AGTTGACCGTAGCAGATTCC+TGG - chr7_1:40708728-40708747 None:intergenic 50.0%
GGAATCTGCTACGGTCAACT+AGG + chr7_1:40708728-40708747 Msa1019270:CDS 50.0%
!! GGTAACGGATTAGGGTCTGT+GGG - chr7_1:40708970-40708989 None:intergenic 50.0%
!! AGGTAACGGATTAGGGTCTG+TGG - chr7_1:40708971-40708990 None:intergenic 50.0%
!! CTACGAGGAGGTAACGGATT+AGG - chr7_1:40708979-40708998 None:intergenic 50.0%
CTAATCCGTTACCTCCTCGT+AGG + chr7_1:40708977-40708996 Msa1019270:CDS 50.0%
CGTTTCCTACGAGGAGGTAA+CGG - chr7_1:40708985-40709004 None:intergenic 50.0%
AGATGAAGCCACGTGGAGTT+TGG - chr7_1:40709025-40709044 None:intergenic 50.0%
TCGGAGCTCCAAGGGAAAAA+CGG - chr7_1:40709256-40709275 None:intergenic 50.0%
ACGGTTAAAGATCCGGGTTC+GGG + chr7_1:40709325-40709344 Msa1019270:CDS 50.0%
! TAAAGATCCGGGTTCGGGTT+TGG + chr7_1:40709330-40709349 Msa1019270:CDS 50.0%
AAAACCGGTTGTTGGGACAC+CGG + chr7_1:40709540-40709559 Msa1019270:CDS 50.0%
TATTAAACCGGGTCCGGGTA+CGG + chr7_1:40709615-40709634 Msa1019270:CDS 50.0%
!! AGGGAAGATTTGGTGTGTGG+CGG + chr7_1:40709759-40709778 Msa1019270:CDS 50.0%
GGAGTAATGTGACGGCACTA+GGG + chr7_1:40709788-40709807 Msa1019270:CDS 50.0%
GAGTAATGTGACGGCACTAG+GGG + chr7_1:40709789-40709808 Msa1019270:CDS 50.0%
TTGTCGTATGCTTGCTCGCA+AGG + chr7_1:40709817-40709836 Msa1019270:CDS 50.0%
TGTCGTATGCTTGCTCGCAA+GGG + chr7_1:40709818-40709837 Msa1019270:CDS 50.0%
TATGCTTGCTCGCAAGGGAA+CGG + chr7_1:40709823-40709842 Msa1019270:CDS 50.0%
ACTTGTGACCCGATTCAACC+CGG + chr7_1:40709847-40709866 Msa1019270:CDS 50.0%
ACCAAACCCTCTCCGACCAA+TGG + chr7_1:40708918-40708937 Msa1019270:CDS 55.0%
TCCATTGGTCGGAGAGGGTT+TGG - chr7_1:40708922-40708941 None:intergenic 55.0%
CATCCACACCAAACTCCACG+TGG + chr7_1:40709014-40709033 Msa1019270:CDS 55.0%
! AAGCCACGTGGAGTTTGGTG+TGG - chr7_1:40709020-40709039 None:intergenic 55.0%
CGAAACGCAGCGTTTGAAGG+TGG - chr7_1:40709151-40709170 None:intergenic 55.0%
!! GAAACGGGTTGGCCTAATGG+TGG + chr7_1:40709442-40709461 Msa1019270:CDS 55.0%
AGAATCAAACCCGGTCGAGC+CGG - chr7_1:40709562-40709581 None:intergenic 55.0%
TTCGGGGAAGACACCAGAGT+CGG + chr7_1:40709693-40709712 Msa1019270:CDS 55.0%
! AGTGCTGGAAACTCCGACTC+TGG - chr7_1:40709709-40709728 None:intergenic 55.0%
GTTTCCAGCACTTCCTCCAC+CGG + chr7_1:40709717-40709736 Msa1019270:CDS 55.0%
GGCTCATTATTCTCCGGTGG+AGG - chr7_1:40709733-40709752 None:intergenic 55.0%
GGTGCGTGGGAGTAATGTGA+CGG + chr7_1:40709780-40709799 Msa1019270:CDS 55.0%
GGGAGTAATGTGACGGCACT+AGG + chr7_1:40709787-40709806 Msa1019270:CDS 55.0%
TAATGTGACGGCACTAGGGG+AGG + chr7_1:40709792-40709811 Msa1019270:CDS 55.0%
ATGCTTGCTCGCAAGGGAAC+GGG + chr7_1:40709824-40709843 Msa1019270:CDS 55.0%
CTTGTGACCCGATTCAACCC+GGG + chr7_1:40709848-40709867 Msa1019270:CDS 55.0%
AGGGACGAGATGAAGCCACG+TGG - chr7_1:40709032-40709051 None:intergenic 60.0%
TTTCCCTTGGAGCTCCGACC+CGG + chr7_1:40709258-40709277 Msa1019270:CDS 60.0%
TAACCGGGTCGGAGCTCCAA+GGG - chr7_1:40709264-40709283 None:intergenic 60.0%
TTAACCGGGTCGGAGCTCCA+AGG - chr7_1:40709265-40709284 None:intergenic 60.0%
!! GCGGAAACGGGTTGGCCTAA+TGG + chr7_1:40709439-40709458 Msa1019270:CDS 60.0%
CCAAAATGCCGCTCCGTACC+CGG - chr7_1:40709631-40709650 None:intergenic 60.0%
! CGGGTACGGAGCGGCATTTT+GGG + chr7_1:40709629-40709648 Msa1019270:CDS 60.0%
! TTCTCCGGTGGAGGAAGTGC+TGG - chr7_1:40709724-40709743 None:intergenic 60.0%
!! ATTTGGTGTGTGGCGGTGCG+TGG + chr7_1:40709766-40709785 Msa1019270:CDS 60.0%
! TTTGGTGTGTGGCGGTGCGT+GGG + chr7_1:40709767-40709786 Msa1019270:CDS 60.0%
CGAGCCGGTGTCCCAACAAC+CGG - chr7_1:40709547-40709566 None:intergenic 65.0%
!! TGTTGGGACACCGGCTCGAC+CGG + chr7_1:40709549-40709568 Msa1019270:CDS 65.0%
! CCGGGTACGGAGCGGCATTT+TGG + chr7_1:40709628-40709647 Msa1019270:CDS 65.0%
!! GTTGGGACACCGGCTCGACC+GGG + chr7_1:40709550-40709569 Msa1019270:CDS 70.0%
AACCGGGTCCGGGTACGGAG+CGG + chr7_1:40709620-40709639 Msa1019270:CDS 70.0%
TGCCGCTCCGTACCCGGACC+CGG - chr7_1:40709625-40709644 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 40708152 40710857 40708152 ID=Msa1019270;Name=Msa1019270
chr7_1 mRNA 40708152 40710857 40708152 ID=Msa1019270-mRNA-1;Parent=Msa1019270;Name=Msa1019270-mRNA-1;_AED=0.01;_eAED=0.01;_QI=365|0.5|0.66|1|1|1|3|332|474
chr7_1 exon 40708152 40708568 40708152 ID=Msa1019270-mRNA-1:exon:7920;Parent=Msa1019270-mRNA-1
chr7_1 exon 40708702 40710007 40708702 ID=Msa1019270-mRNA-1:exon:7921;Parent=Msa1019270-mRNA-1
chr7_1 exon 40710459 40710857 40710459 ID=Msa1019270-mRNA-1:exon:7922;Parent=Msa1019270-mRNA-1
chr7_1 five_prime_UTR 40708152 40708516 40708152 ID=Msa1019270-mRNA-1:five_prime_utr;Parent=Msa1019270-mRNA-1
chr7_1 CDS 40708517 40708568 40708517 ID=Msa1019270-mRNA-1:cds;Parent=Msa1019270-mRNA-1
chr7_1 CDS 40708702 40710007 40708702 ID=Msa1019270-mRNA-1:cds;Parent=Msa1019270-mRNA-1
chr7_1 CDS 40710459 40710525 40710459 ID=Msa1019270-mRNA-1:cds;Parent=Msa1019270-mRNA-1
chr7_1 three_prime_UTR 40710526 40710857 40710526 ID=Msa1019270-mRNA-1:three_prime_utr;Parent=Msa1019270-mRNA-1
Gene Sequence

>Msa1019270

ATGGCTTTGTTTCTCCTCTTTCTCTTCTCCATTCTACTCAACAACATTTCAAGTAGCTCAGCTTTTTCATCACAACCAGGAATCTGCTACGGTCAACTAGGAAACAACCTACCAAAACCCTCAAAATCACTCTCCTTAATCAAATCCTTAAACGCAAAGCGCATCAAAATCTATGACACAAACCCACAAATCCTAACAACCTTAAAAAACACAAACATCCAAATCTCAATCATGCTCCCAAACGAACTCATCACAAACATCTCAACAAACCAAACCCTCTCCGACCAATGGATCAAATCCAACATTCTACCTTATTACCCACAGACCCTAATCCGTTACCTCCTCGTAGGAAACGAAATCATCTCATCCACACCAAACTCCACGTGGCTTCATCTCGTCCCTGCGATGCGTAGAATTAAACACTCTTTGAAAAAACTCAGAATCAAAAGAATCAAAGTTGGTACTTCTTCTGCTATGGATGTTCTAGAAACTTCTTTTCCACCTTCAAACGCTGCGTTTCGTAAAGATATAGCGGTTCAAGTTATTAAACCGATGCTTAGGTTTTTAAACCGGACTAAATCTTTTTTCTTTTTGGATGTTTATCCGTTTTTCCCTTGGAGCTCCGACCCGGTTAATGTTAATTTGGATTATGCTTTGTTTAGAGAGAGTAATGTTACGGTTAAAGATCCGGGTTCGGGTTTGGTTTATACGAATTTATTTGATCAGATGGTGGATGCGGTTTATTTTGCGATGGAGAAACTTGGGTTTCCGGATGTTCGGATATTTATCGCGGAAACGGGTTGGCCTAATGGTGGTGATGTTGATCAAATTGGTGCTAATGTTTTTAATGCTGCTACTTATAATCGTAATTTTGTTAAAAAAGTTATGAAAAAACCGGTTGTTGGGACACCGGCTCGACCGGGTTTGATTCTTCCGAGTTTTTTATTTGCTTTGTTTAATGAGAATATTAAACCGGGTCCGGGTACGGAGCGGCATTTTGGGTTGTTGTATCCAAATGGGTCGAGGGTTTATGAGATTGATCTTTCGGGGAAGACACCAGAGTCGGAGTTTCCAGCACTTCCTCCACCGGAGAATAATGAGCCATATAAAGGGAAGATTTGGTGTGTGGCGGTGCGTGGGAGTAATGTGACGGCACTAGGGGAGGCATTGTCGTATGCTTGCTCGCAAGGGAACGGGACTTGTGACCCGATTCAACCCGGGAAAAAGTGTTTTAAGCCCGATTCGGATTTTTGGCATGCTAGCTATGCGTTTAGTTCTTATTGGGCACAGTTTAAGAAGACTGGAGGAACTTGTTACTTTAATGGGCTTGCTACTCAAACTGCAAAGGATCCAAGTAAGTTATGGATCTTGCAAGTTTCCAAGTGTGACACTTTGAAGGAGGAGCTCTCTTCATCCTCCATTTGA
Protein sequence

>Msa1019270

MALFLLFLFSILLNNISSSSAFSSQPGICYGQLGNNLPKPSKSLSLIKSLNAKRIKIYDTNPQILTTLKNTNIQISIMLPNELITNISTNQTLSDQWIKSNILPYYPQTLIRYLLVGNEIISSTPNSTWLHLVPAMRRIKHSLKKLRIKRIKVGTSSAMDVLETSFPPSNAAFRKDIAVQVIKPMLRFLNRTKSFFFLDVYPFFPWSSDPVNVNLDYALFRESNVTVKDPGSGLVYTNLFDQMVDAVYFAMEKLGFPDVRIFIAETGWPNGGDVDQIGANVFNAATYNRNFVKKVMKKPVVGTPARPGLILPSFLFALFNENIKPGPGTERHFGLLYPNGSRVYEIDLSGKTPESEFPALPPPENNEPYKGKIWCVAVRGSNVTALGEALSYACSQGNGTCDPIQPGKKCFKPDSDFWHASYAFSSYWAQFKKTGGTCYFNGLATQTAKDPSKLWILQVSKCDTLKEELSSSSI*