AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1104220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1104220 MtrunA17_Chr7g0234821 96.328 463 17 0 1 463 1 463 0.0 925
Msa1104220 MtrunA17_Chr8g0339191 71.922 463 125 2 1 463 6 463 0.0 712
Msa1104220 MtrunA17_Chr4g0046311 54.286 455 198 3 1 452 4 451 0.0 546
Msa1104220 MtrunA17_Chr1g0177171 41.485 458 240 7 27 460 39 492 4.87e-124 370
Msa1104220 MtrunA17_Chr5g0439251 40.319 439 245 5 27 452 26 460 1.15e-115 348
Msa1104220 MtrunA17_Chr6g0487261 39.130 460 263 9 4 458 7 454 1.45e-113 343
Msa1104220 MtrunA17_Chr8g0389741 39.723 433 251 6 27 458 29 452 6.18e-111 337
Msa1104220 MtrunA17_Chr4g0034901 37.529 437 265 6 27 460 30 461 3.33e-104 318
Msa1104220 MtrunA17_Chr3g0144531 37.310 461 269 9 4 458 8 454 4.90e-100 307
Msa1104220 MtrunA17_Chr4g0003281 42.297 357 194 6 3 355 18 366 1.19e-96 296
Msa1104220 MtrunA17_Chr3g0110371 35.129 464 276 8 1 459 3 446 4.31e-95 295
Msa1104220 MtrunA17_Chr4g0075031 38.073 436 253 7 27 459 46 467 1.05e-94 295
Msa1104220 MtrunA17_Chr8g0378621 34.862 436 268 6 27 459 27 449 9.16e-93 290
Msa1104220 MtrunA17_Chr1g0161011 33.623 461 287 8 1 458 1 445 1.55e-90 284
Msa1104220 MtrunA17_Chr5g0434591 34.194 465 280 10 1 459 1 445 1.22e-89 281
Msa1104220 MtrunA17_Chr3g0119541 42.508 327 183 4 21 346 21 343 1.14e-88 275
Msa1104220 MtrunA17_Chr3g0121271 33.477 463 277 10 2 458 9 446 4.38e-88 277
Msa1104220 MtrunA17_Chr4g0043851 42.769 325 180 5 27 350 33 352 5.07e-88 274
Msa1104220 MtrunA17_Chr4g0026321 40.719 334 192 5 27 359 30 358 7.55e-88 273
Msa1104220 MtrunA17_Chr4g0043511 36.009 461 269 11 18 459 51 504 1.79e-86 275
Msa1104220 MtrunA17_Chr2g0285901 40.517 348 200 5 1 348 1 341 1.82e-85 268
Msa1104220 MtrunA17_Chr2g0321511 40.762 341 183 8 27 360 30 358 3.83e-85 266
Msa1104220 MtrunA17_Chr7g0223961 37.130 439 255 11 27 458 24 448 5.57e-84 265
Msa1104220 MtrunA17_Chr1g0146831 45.171 321 166 6 27 345 32 344 1.87e-83 261
Msa1104220 MtrunA17_Chr1g0189111 41.049 324 184 5 27 349 32 349 1.27e-80 256
Msa1104220 MtrunA17_Chr4g0064021 37.015 335 206 4 17 350 17 347 8.18e-80 253
Msa1104220 MtrunA17_Chr1g0155001 38.462 325 194 5 27 349 25 345 1.49e-71 232
Msa1104220 MtrunA17_Chr2g0294591 35.650 331 199 8 23 351 27 345 1.45e-66 217
Msa1104220 MtrunA17_Chr2g0294601 36.080 352 204 11 3 351 41 374 1.22e-65 216
Msa1104220 MtrunA17_Chr4g0038981 36.646 322 181 10 27 345 24 325 6.05e-65 212
Msa1104220 MtrunA17_Chr4g0038941 36.728 324 186 8 27 349 53 358 1.56e-63 209
Msa1104220 MtrunA17_Chr7g0250301 35.535 318 193 6 27 344 9 314 7.20e-63 206
Msa1104220 MtrunA17_Chr4g0039011 34.412 340 201 9 14 350 30 350 1.75e-62 207
Msa1104220 MtrunA17_Chr4g0038961 37.461 323 179 10 27 346 24 326 2.29e-62 205
Msa1104220 MtrunA17_Chr8g0383311 32.653 441 272 16 27 459 44 467 1.30e-61 208
Msa1104220 MtrunA17_Chr2g0306311 29.980 497 284 18 1 459 12 482 3.40e-61 207
Msa1104220 MtrunA17_Chr4g0039071 34.579 321 198 6 27 346 26 335 5.83e-61 203
Msa1104220 MtrunA17_Chr4g0038971 36.533 323 182 10 27 346 24 326 1.40e-60 201
Msa1104220 MtrunA17_Chr1g0174261 37.755 294 169 7 55 346 1 282 3.38e-59 197
Msa1104220 MtrunA17_Chr4g0062771 30.064 469 294 19 4 459 10 457 2.66e-57 197
Msa1104220 MtrunA17_Chr1g0148391 30.717 446 271 17 27 458 27 448 6.02e-57 195
Msa1104220 MtrunA17_Chr3g0138741 30.687 466 287 17 3 458 15 454 1.23e-56 195
Msa1104220 MtrunA17_Chr4g0069801 31.106 434 265 18 38 458 39 451 1.84e-55 192
Msa1104220 MtrunA17_Chr2g0290821 31.839 446 278 16 21 458 23 450 1.86e-55 192
Msa1104220 MtrunA17_Chr4g0039021 36.301 292 168 8 53 341 8 284 2.73e-55 186
Msa1104220 MtrunA17_Chr3g0129341 30.682 440 280 16 27 459 35 456 1.09e-54 189
Msa1104220 MtrunA17_Chr7g0250321 35.955 267 162 4 27 293 29 286 6.42e-52 177
Msa1104220 MtrunA17_Chr4g0076581 31.011 445 278 16 24 458 31 456 1.43e-50 179
Msa1104220 MtrunA17_Chr5g0436361 35.948 306 184 8 47 349 58 354 1.52e-50 176
Msa1104220 MtrunA17_Chr3g0105981 28.009 432 281 16 20 443 21 430 6.00e-47 167
Msa1104220 MtrunA17_Chr6g0461601 26.966 445 294 17 27 458 27 453 2.25e-43 159
Msa1104220 MtrunA17_Chr7g0253091 26.180 466 310 19 6 458 6 450 3.48e-41 153
Msa1104220 MtrunA17_Chr5g0401301 22.970 431 269 10 51 458 24 414 3.37e-27 113
Msa1104220 MtrunA17_Chr2g0294581 34.302 172 102 4 23 190 73 237 5.38e-25 104
Msa1104220 MtrunA17_Chr5g0447651 41.414 99 55 1 360 458 19 114 1.52e-20 87.0
Msa1104220 MtrunA17_Chr1g0213651 47.191 89 46 1 370 458 272 359 3.20e-20 92.0
Msa1104220 MtrunA17_Chr8g0378381 44.444 90 49 1 370 459 28 116 1.36e-19 84.3
Msa1104220 MtrunA17_Chr4g0076131 41.176 85 50 0 374 458 28 112 6.37e-19 82.4
Msa1104220 MtrunA17_Chr8g0364811 47.561 82 43 0 129 210 2 83 1.61e-18 80.5
Msa1104220 MtrunA17_Chr8g0378361 46.067 89 47 1 374 462 30 117 2.02e-18 83.2
Msa1104220 MtrunA17_Chr8g0381081 39.216 102 61 1 360 460 42 143 1.32e-17 80.5
Msa1104220 MtrunA17_Chr3g0120401 44.330 97 53 1 365 461 148 243 1.93e-17 81.6
Msa1104220 MtrunA17_Chr3g0096451 38.532 109 65 2 352 459 89 196 2.53e-17 81.6
Msa1104220 MtrunA17_Chr8g0378371 43.820 89 46 2 372 460 31 115 9.69e-17 76.3
Msa1104220 MtrunA17_Chr4g0040911 43.956 91 51 0 370 460 33 123 1.67e-16 77.4
Msa1104220 MtrunA17_Chr8g0378401 43.373 83 46 1 370 452 28 109 1.83e-15 72.8
Msa1104220 MtrunA17_Chr4g0018771 40.206 97 51 2 250 346 86 175 2.51e-15 75.1
Msa1104220 MtrunA17_Chr4g0018861 40.196 102 54 2 245 346 79 173 2.59e-15 75.1
Msa1104220 MtrunA17_Chr4g0018861 35.227 88 57 0 5 92 1 88 4.33e-11 62.8
Msa1104220 MtrunA17_Chr5g0443331 38.710 93 56 1 370 462 104 195 2.61e-15 75.9
Msa1104220 MtrunA17_Chr4g0076141 40.506 79 47 0 374 452 13 91 3.34e-15 71.2
Msa1104220 MtrunA17_Chr2g0304061 43.529 85 46 2 374 458 158 240 1.11e-13 72.0
Msa1104220 MtrunA17_Chr1g0154991 38.235 102 59 3 358 458 5 103 1.88e-13 66.6
Msa1104220 MtrunA17_Chr7g0271411 40.698 86 50 1 374 459 160 244 5.14e-13 69.7
Msa1104220 MtrunA17_Chr1g0193131 40.000 85 49 2 370 454 416 498 5.31e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1104220 AT3G07320.1 65.919 446 150 1 18 463 17 460 0.0 635
Msa1104220 AT3G23770.1 56.054 446 192 2 21 463 32 476 0.0 541
Msa1104220 AT4G14080.1 52.192 479 212 2 1 463 1 478 0.0 536
Msa1104220 AT4G26830.1 41.791 469 248 8 1 460 1 453 3.69e-129 382
Msa1104220 AT4G26830.2 42.222 450 236 7 20 460 23 457 4.31e-127 377
Msa1104220 AT5G55180.1 42.188 448 240 7 21 460 22 458 6.21e-126 374
Msa1104220 AT5G55180.2 42.045 440 236 7 21 452 22 450 6.81e-121 361
Msa1104220 AT2G05790.1 39.644 449 252 7 27 460 26 470 1.92e-116 350
Msa1104220 AT5G56590.1 39.318 440 258 6 21 458 21 453 6.55e-110 334
Msa1104220 AT4G29360.2 38.831 479 261 7 4 458 15 485 4.02e-106 326
Msa1104220 AT4G29360.1 39.177 462 249 7 21 458 22 475 4.13e-106 325
Msa1104220 AT5G24318.1 37.391 460 277 7 1 458 5 455 2.87e-102 313
Msa1104220 AT5G24318.2 37.225 454 274 7 1 452 5 449 2.07e-98 303
Msa1104220 AT4G34480.1 37.259 467 266 10 1 459 1 448 8.82e-96 298
Msa1104220 AT2G01630.1 36.468 436 260 7 27 459 24 445 3.31e-95 296
Msa1104220 AT2G16230.1 37.443 438 256 8 27 459 26 450 8.34e-95 295
Msa1104220 AT2G01630.3 36.131 429 257 7 27 452 24 438 3.31e-92 286
Msa1104220 AT3G55430.1 36.569 443 259 12 21 458 24 449 7.18e-91 283
Msa1104220 AT1G66250.1 34.253 435 268 8 27 458 33 452 5.48e-88 278
Msa1104220 AT3G13560.4 34.023 435 269 7 27 458 27 446 1.82e-87 276
Msa1104220 AT3G13560.1 34.023 435 269 7 27 458 27 446 1.82e-87 276
Msa1104220 AT3G13560.2 34.023 435 269 7 27 458 27 446 1.82e-87 276
Msa1104220 AT3G13560.5 34.023 435 269 7 27 458 27 446 1.82e-87 276
Msa1104220 AT3G13560.3 34.023 435 269 7 27 458 27 446 1.82e-87 276
Msa1104220 AT1G11820.2 33.945 436 271 7 27 459 45 466 2.07e-87 276
Msa1104220 AT3G15800.1 40.299 335 194 5 18 350 35 365 1.62e-84 265
Msa1104220 AT4G18340.2 40.741 324 186 5 27 349 35 353 2.13e-83 262
Msa1104220 AT4G18340.1 40.741 324 186 5 27 349 35 353 5.22e-83 261
Msa1104220 AT1G11820.3 33.333 429 269 7 27 452 45 459 6.24e-83 264
Msa1104220 AT2G27500.2 38.298 329 198 4 27 354 30 354 7.32e-82 258
Msa1104220 AT2G27500.1 38.298 329 198 4 27 354 30 354 2.55e-81 257
Msa1104220 AT1G30080.2 39.535 344 202 5 4 346 14 352 1.17e-80 255
Msa1104220 AT1G30080.1 39.535 344 202 5 4 346 14 352 1.72e-80 255
Msa1104220 AT1G32860.1 40.187 321 188 4 27 346 30 347 3.37e-80 255
Msa1104220 AT5G24318.4 39.429 350 202 6 1 348 5 346 5.72e-80 253
Msa1104220 AT5G42720.1 40.793 353 198 7 2 352 7 350 1.53e-79 254
Msa1104220 AT2G39640.1 35.217 460 270 14 4 459 8 443 1.04e-75 247
Msa1104220 AT3G57270.1 39.877 326 182 7 25 348 26 339 2.34e-75 240
Msa1104220 AT2G26600.1 35.398 339 211 5 21 355 29 363 3.47e-75 241
Msa1104220 AT5G42100.2 39.329 328 192 6 24 348 25 348 8.57e-75 241
Msa1104220 AT5G42100.1 39.329 328 192 6 24 348 25 348 1.37e-74 241
Msa1104220 AT4G34480.2 38.333 360 211 7 1 356 1 353 1.60e-74 238
Msa1104220 AT2G01630.2 35.526 380 228 7 27 403 24 389 7.64e-73 235
Msa1104220 AT3G61810.1 37.463 339 194 6 18 349 45 372 9.53e-73 234
Msa1104220 AT5G24318.3 39.355 310 180 4 149 458 15 316 1.76e-71 229
Msa1104220 AT4G34480.3 38.177 351 196 7 114 459 3 337 5.06e-70 228
Msa1104220 AT4G16260.1 37.243 341 191 11 3 339 4 325 4.34e-67 218
Msa1104220 AT3G46570.1 37.309 327 194 7 25 348 26 344 2.86e-64 211
Msa1104220 AT2G27500.3 37.634 279 169 4 77 354 2 276 1.47e-63 208
Msa1104220 AT5G20390.1 37.202 336 197 9 4 338 12 334 2.10e-63 209
Msa1104220 AT1G33220.1 36.747 332 194 9 3 330 8 327 6.35e-63 207
Msa1104220 AT5G20340.1 37.539 317 185 8 27 342 44 348 1.68e-62 207
Msa1104220 AT5G20330.1 36.103 349 206 10 3 346 8 344 2.14e-62 206
Msa1104220 AT3G57260.1 34.104 346 210 8 4 349 12 339 4.86e-62 205
Msa1104220 AT4G16260.2 37.217 309 178 9 33 339 2 296 8.91e-62 204
Msa1104220 AT3G57260.2 34.393 346 209 8 4 349 28 355 9.65e-62 205
Msa1104220 AT3G24330.1 30.603 464 297 16 5 459 18 465 8.28e-61 206
Msa1104220 AT2G26600.2 36.630 273 165 5 87 355 1 269 6.46e-60 198
Msa1104220 AT1G11820.1 33.333 327 211 5 27 350 45 367 1.80e-59 200
Msa1104220 AT1G77780.1 33.140 344 218 7 3 346 1 332 1.25e-57 194
Msa1104220 AT3G57240.1 34.494 316 188 8 25 339 34 331 5.30e-56 189
Msa1104220 AT4G31140.1 28.959 442 283 17 27 458 28 448 9.81e-55 190
Msa1104220 AT5G20870.1 30.380 474 289 21 4 459 10 460 1.93e-51 182
Msa1104220 AT4G17180.1 30.435 460 285 17 6 458 9 440 1.41e-50 179
Msa1104220 AT3G55780.1 33.016 315 197 8 40 349 49 354 3.12e-50 175
Msa1104220 AT5G18220.1 29.039 458 290 18 16 459 20 456 2.79e-49 176
Msa1104220 AT5G20560.1 35.673 342 201 11 3 338 8 336 6.89e-49 171
Msa1104220 AT1G77790.1 31.250 320 211 5 27 346 31 341 9.67e-49 171
Msa1104220 AT5G58090.1 29.306 447 287 17 23 459 19 446 2.02e-47 170
Msa1104220 AT3G04010.1 28.294 463 296 19 11 459 18 458 1.50e-46 168
Msa1104220 AT5G64790.1 27.252 444 292 15 27 459 29 452 1.72e-46 168
Msa1104220 AT2G19440.2 28.442 443 288 19 27 459 22 445 7.43e-43 158
Msa1104220 AT2G19440.1 28.442 443 288 19 27 459 22 445 7.43e-43 158
Msa1104220 AT5G58480.1 27.191 445 284 16 27 458 27 444 1.06e-42 157
Msa1104220 AT1G64760.1 27.928 444 289 20 27 459 26 449 1.41e-41 154
Msa1104220 AT1G64760.2 27.928 444 289 20 27 459 26 449 1.41e-41 154
Msa1104220 AT5G67460.1 42.991 107 55 2 358 458 271 377 7.19e-22 97.4
Msa1104220 AT5G67460.2 42.574 101 52 2 358 452 271 371 6.96e-19 88.6
Msa1104220 AT5G35740.1 38.614 101 60 2 359 458 16 115 3.77e-18 80.5
Msa1104220 AT4G13600.1 47.126 87 45 1 373 459 60 145 4.80e-17 80.5
Msa1104220 AT4G05430.1 36.449 107 63 2 353 458 5 107 5.73e-17 78.2
Msa1104220 AT2G04910.2 39.773 88 53 0 374 461 15 102 8.47e-17 76.3
Msa1104220 AT2G30933.1 43.820 89 49 1 374 462 82 169 2.86e-16 78.2
Msa1104220 AT1G79480.2 41.176 85 49 1 374 458 310 393 6.69e-16 79.7
Msa1104220 AT1G79480.1 41.176 85 49 1 374 458 311 394 6.79e-16 79.7
Msa1104220 AT5G61130.1 41.860 86 49 1 374 459 21 105 9.96e-16 76.3
Msa1104220 AT1G66870.2 35.227 88 57 0 371 458 50 137 1.53e-15 73.9
Msa1104220 AT4G05430.3 35.780 109 65 2 353 460 30 134 1.67e-15 74.7
Msa1104220 AT4G09462.1 36.842 95 59 1 366 460 21 114 2.80e-15 72.4
Msa1104220 AT1G66870.1 35.227 88 57 0 371 458 23 110 2.91e-15 72.4
Msa1104220 AT1G29380.1 41.176 85 49 1 374 458 148 231 1.70e-14 74.7
Msa1104220 AT1G29380.3 41.379 87 50 1 374 460 148 233 2.18e-14 73.6
Msa1104220 AT4G09465.1 37.079 89 55 1 372 460 29 116 2.42e-14 69.7
Msa1104220 AT4G09464.1 37.079 89 55 1 372 460 29 116 2.54e-14 69.7
Msa1104220 AT4G09467.1 37.079 89 55 1 372 460 29 116 2.54e-14 69.7
Msa1104220 AT1G18650.1 39.130 92 55 1 369 460 16 106 2.93e-14 71.6
Msa1104220 AT5G63250.1 33.333 108 66 2 355 460 27 130 3.34e-14 69.7
Msa1104220 AT3G58100.1 37.079 89 56 0 372 460 39 127 4.00e-14 70.9
Msa1104220 AT2G29735.1 37.363 91 54 2 370 458 27 116 4.85e-14 68.9
Msa1104220 AT3G58100.2 37.079 89 56 0 372 460 115 203 6.28e-14 72.0
Msa1104220 AT1G18650.2 39.130 92 55 1 369 460 16 106 1.29e-13 68.2
Msa1104220 AT4G05430.2 35.644 101 60 2 353 452 30 126 1.68e-13 67.8
Msa1104220 AT2G04910.1 39.241 79 48 0 374 452 15 93 4.76e-13 66.2
Msa1104220 AT5G63225.1 35.294 85 54 1 374 458 25 108 7.02e-13 65.5
Msa1104220 AT1G66852.1 35.227 88 56 1 371 458 23 109 8.28e-13 65.1
Msa1104220 AT4G09466.1 34.831 89 57 1 372 460 29 116 9.45e-13 65.1
Msa1104220 AT1G09460.1 38.947 95 55 2 364 458 127 218 4.19e-12 67.8
Msa1104220 AT2G30933.2 41.772 79 45 1 374 452 82 159 5.35e-12 64.3
Msa1104220 AT2G30933.3 41.772 79 45 1 374 452 82 159 5.35e-12 64.3
Msa1104220 AT1G66855.1 30.682 88 61 0 371 458 23 110 6.30e-12 62.8
Msa1104220 AT5G08000.2 39.326 89 53 1 371 459 37 124 6.39e-12 65.5
Msa1104220 AT4G16165.1 34.831 89 58 0 370 458 22 110 1.20e-11 62.0
Msa1104220 AT1G29380.4 39.241 79 47 1 374 452 148 225 1.30e-11 64.7
Msa1104220 AT1G29380.2 39.241 79 47 1 374 452 148 225 1.30e-11 64.7
Msa1104220 AT5G63230.1 32.941 85 56 1 374 458 26 109 2.34e-11 61.2
Msa1104220 AT4G09090.1 33.333 87 57 1 374 460 31 116 2.57e-11 61.2
Msa1104220 AT5G53600.1 31.034 87 58 1 372 458 27 111 4.15e-11 60.5
Msa1104220 AT5G63240.1 33.333 90 58 1 373 460 39 128 4.78e-11 60.8
Msa1104220 AT5G08000.1 39.535 86 51 1 374 459 21 105 4.93e-11 62.4

Find 155 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGATGCGCTTTGCGTTTA+AGG 0.094987 7_3:-41234750 None:intergenic
CACCAAATCTTTCCTTTATA+TGG 0.159160 7_3:-41235706 None:intergenic
CGACCCGGTTAATGTTAATT+TGG 0.198547 7_3:+41235228 Msa1104220:CDS
GGAGGAACTTGTTACTTTAA+TGG 0.203799 7_3:+41235907 Msa1104220:CDS
TATAAGAGGAAATTTAAATT+TGG 0.215220 7_3:+41236349 Msa1104220:three_prime_UTR
GATGGAGAAACTTGGGTTTC+CGG 0.239471 7_3:+41235354 Msa1104220:CDS
TACGAGGAGGTAACGGATTA+AGG 0.240467 7_3:-41234930 None:intergenic
ATTAAGGTTTGTGGGTAATA+AGG 0.281507 7_3:-41234914 None:intergenic
TACGGTTAAGGATCCGGGTT+CGG 0.285504 7_3:+41235279 Msa1104220:CDS
GTTTATGAGATTGATCTTTC+GGG 0.289787 7_3:+41235631 Msa1104220:CDS
GGTTTATGAGATTGATCTTT+CGG 0.293706 7_3:+41235630 Msa1104220:CDS
TAAGGATCCGGGTTCGGGTT+TGG 0.304227 7_3:+41235285 Msa1104220:CDS
TAATCCAAATTAACATTAAC+CGG 0.304337 7_3:-41235232 None:intergenic
TTTAAGGTTGTTAGGATTTG+TGG 0.304363 7_3:-41234788 None:intergenic
ACGGTTAAGGATCCGGGTTC+GGG 0.304864 7_3:+41235280 Msa1104220:CDS
AGCATCATGATTTCAAATAA+TGG 0.307100 7_3:-41236549 None:intergenic
TGTTGTATCCGAACGGGTCT+AGG 0.311397 7_3:+41235608 Msa1104220:CDS
GAGGAACTTGTTACTTTAAT+GGG 0.332834 7_3:+41235908 Msa1104220:CDS
GGGAGCAATGTGACAGCGTT+AGG 0.342581 7_3:+41235742 Msa1104220:CDS
TTAATTGACATTTCTATAAG+AGG 0.349394 7_3:+41236335 Msa1104220:three_prime_UTR
CGGATATTTATCGCGGAAAC+GGG 0.349889 7_3:+41235382 Msa1104220:CDS
GCTATGCGTTTAGTTCCTAT+TGG 0.358375 7_3:+41235866 Msa1104220:CDS
TTGCAACACCTGGATTCATT+TGG 0.358963 7_3:+41236631 Msa1104220:three_prime_UTR
GAGAATAATGAGCCATATAA+AGG 0.363137 7_3:+41235694 Msa1104220:CDS
TCCATTGGTCGGAGAGGGTT+TGG 0.366090 7_3:-41234874 None:intergenic
AATGTAAGTTCCTCCTTTGA+AGG 0.366921 7_3:-41236442 None:intergenic
CTTCTTAAACTGTGCCCAAT+AGG 0.366956 7_3:-41235881 None:intergenic
CTATGCGTTTAGTTCCTATT+GGG 0.381942 7_3:+41235867 Msa1104220:CDS
GCGGAAACGGGTTGGCCTAA+TGG 0.386035 7_3:+41235394 Msa1104220:CDS
TTGGGAGCATGATTGAGATT+TGG 0.386715 7_3:-41234823 None:intergenic
AGTTGACCGTAGCAGATTCC+TGG 0.387463 7_3:-41234680 None:intergenic
TGCGTTTAAGGATTTGATTA+AGG 0.388788 7_3:-41234738 None:intergenic
TTAAGGTTGTTAGGATTTGT+GGG 0.390167 7_3:-41234787 None:intergenic
AGATGAAGCCACGTGGAGTT+TGG 0.393557 7_3:-41234977 None:intergenic
ACACAACACAACCCATTTCC+AGG 0.394582 7_3:+41234267 Msa1104220:five_prime_UTR
AACTTGGGTTTCCGGATGTT+CGG 0.396533 7_3:+41235362 Msa1104220:CDS
AGAGAGTAATGTTACGGTTA+AGG 0.398626 7_3:+41235267 Msa1104220:CDS
ATGCTTGCTCGCAGGGGAAC+CGG 0.399988 7_3:+41235779 Msa1104220:CDS
GTTTAATGAGAATATTAAAC+CGG 0.401130 7_3:+41235558 Msa1104220:CDS
CCAAAATGCCGCTCCGTACC+TGG 0.402042 7_3:-41235583 None:intergenic
TAATGTTACGGTTAAGGATC+CGG 0.405432 7_3:+41235273 Msa1104220:CDS
GGTGGTGATGTTGATCAAAT+TGG 0.406359 7_3:+41235415 Msa1104220:CDS
AACAAAATTTCTATTATAAG+TGG 0.407690 7_3:-41235458 None:intergenic
AGATGGATGGACCCTGGAAA+TGG 0.412293 7_3:-41234279 None:intergenic
TCGGATATTTATCGCGGAAA+CGG 0.419442 7_3:+41235381 Msa1104220:CDS
AGCCATATAAAGGAAAGATT+TGG 0.420676 7_3:+41235704 Msa1104220:CDS
ATGTTTGTGATGAGTTCGTT+TGG 0.429565 7_3:-41234842 None:intergenic
TGTTGGGACACCGGCTAGAC+CGG 0.437582 7_3:+41235504 Msa1104220:CDS
TTGGGTTGTTGTATCCGAAC+GGG 0.438048 7_3:+41235602 Msa1104220:CDS
TGAAATGTTGTTGAGTAGAA+TGG 0.438583 7_3:-41234500 None:intergenic
TACGAATTTATTTGATCAGA+TGG 0.440268 7_3:+41235312 Msa1104220:CDS
AGTTGGTACTTCTTCTGCTA+TGG 0.450725 7_3:+41235060 Msa1104220:CDS
AGTGCTGGAAACTCCGACTC+CGG 0.454147 7_3:-41235661 None:intergenic
TTACGAAACGCAGCGTTTGA+AGG 0.455167 7_3:-41235106 None:intergenic
TTCTCCGGTGGAGGAAGTGC+TGG 0.459187 7_3:-41235676 None:intergenic
TGTTTGTGATGAGTTCGTTT+GGG 0.471998 7_3:-41234841 None:intergenic
ACACCTGGATTCATTTGGAT+TGG 0.472515 7_3:+41236636 Msa1104220:three_prime_UTR
AGAAGATTGAGATGAGACTA+TGG 0.473878 7_3:-41234239 None:intergenic
TGGGCACAGTTTAAGAAGAC+TGG 0.479411 7_3:+41235886 Msa1104220:CDS
TTGACGTATGCTTGCTCGCA+GGG 0.481295 7_3:+41235772 Msa1104220:CDS
TTATATGGCTCATTATTCTC+CGG 0.483598 7_3:-41235691 None:intergenic
CAATTAATGAATGATTCAAA+TGG 0.485467 7_3:-41236319 None:intergenic
GGAGCAATGTGACAGCGTTA+GGG 0.486437 7_3:+41235743 Msa1104220:CDS
TTCCAAGTGTGACACTTTGA+AGG 0.487564 7_3:+41236278 Msa1104220:CDS
ACTATGGTGGTTTGATAAAG+TGG 0.487735 7_3:-41234223 None:intergenic
CGTTTCCTACGAGGAGGTAA+CGG 0.487887 7_3:-41234937 None:intergenic
AGGTAACGGATTAAGGTTTG+TGG 0.488254 7_3:-41234923 None:intergenic
CTCCTTCAAAGTGTCACACT+TGG 0.488468 7_3:-41236280 None:intergenic
GTTTAGAGAGAGTAATGTTA+CGG 0.488551 7_3:+41235261 Msa1104220:CDS
CTAGCCGGTGTCCCAACAAC+CGG 0.490810 7_3:-41235499 None:intergenic
ATAAAAGATAAAGTAGGAGA+TGG 0.491479 7_3:-41234296 None:intergenic
TGATCAACATCACCACCATT+AGG 0.494772 7_3:-41235409 None:intergenic
TATTCATTTATTGCAACACC+TGG 0.497950 7_3:+41236621 Msa1104220:three_prime_UTR
AAGTTATTAAGCCCATGCTT+AGG 0.501182 7_3:+41235143 Msa1104220:CDS
GTAGAATGGAGAAGAGAAAG+AGG 0.501465 7_3:-41234486 None:intergenic
GGGGAACCGGACTTGTGATC+CGG 0.503868 7_3:+41235792 Msa1104220:CDS
GAACGTTGGATTTGATCCAT+TGG 0.505085 7_3:-41234889 None:intergenic
CGAAACGCAGCGTTTGAAGG+TGG 0.507919 7_3:-41235103 None:intergenic
TTTGATCCATTGGTCGGAGA+GGG 0.508250 7_3:-41234879 None:intergenic
TTTGGGTTGTTGTATCCGAA+CGG 0.509076 7_3:+41235601 Msa1104220:CDS
GATGGATGGACCCTGGAAAT+GGG 0.510246 7_3:-41234278 None:intergenic
GAGAATATTAAACCGGGTCC+AGG 0.511434 7_3:+41235565 Msa1104220:CDS
CTTTCAGAAGTTGTGCTATG+TGG 0.515432 7_3:-41234421 None:intergenic
GCGATAAATATCCGAACATC+CGG 0.517870 7_3:-41235373 None:intergenic
GGGAAGATAAAAGATAAAGT+AGG 0.522781 7_3:-41234302 None:intergenic
ATTTGGTGTGTGGCGGTGCG+TGG 0.522983 7_3:+41235721 Msa1104220:CDS
AATGTTACGGTTAAGGATCC+GGG 0.524550 7_3:+41235274 Msa1104220:CDS
ATTTGATCAGATGGTGGATG+CGG 0.528077 7_3:+41235321 Msa1104220:CDS
GTTGTATCCGAACGGGTCTA+GGG 0.530355 7_3:+41235609 Msa1104220:CDS
GTTTCCAGCACTTCCTCCAC+CGG 0.531728 7_3:+41235672 Msa1104220:CDS
AGAATTGAACCCGGTCTAGC+CGG 0.532603 7_3:-41235514 None:intergenic
GGCTCATTATTCTCCGGTGG+AGG 0.534146 7_3:-41235685 None:intergenic
GGAATCTGCTACGGTCAACT+AGG 0.534218 7_3:+41234683 Msa1104220:CDS
GGTAACGGATTAAGGTTTGT+GGG 0.534729 7_3:-41234922 None:intergenic
AGAATCAAAAGAATCAAAGT+TGG 0.535473 7_3:+41235043 Msa1104220:CDS
GAAGGTTGAATGTTACTAGA+GGG 0.544294 7_3:-41236424 None:intergenic
TAGTAACATTCAACCTTCAA+AGG 0.544730 7_3:+41236429 Msa1104220:three_prime_UTR
TGTTTGATTCTACGCATCGC+AGG 0.546557 7_3:-41235004 None:intergenic
TGCCGCTCCGTACCTGGACC+CGG 0.546698 7_3:-41235577 None:intergenic
TGGGTAATAAGGTAGAACGT+TGG 0.547510 7_3:-41234903 None:intergenic
TATTTATCGCGGAAACGGGT+TGG 0.547640 7_3:+41235386 Msa1104220:CDS
CTATGGTGGTTTGATAAAGT+GGG 0.549418 7_3:-41234222 None:intergenic
GTTGGGACACCGGCTAGACC+GGG 0.550859 7_3:+41235505 Msa1104220:CDS
TATTAAACCGGGTCCAGGTA+CGG 0.551795 7_3:+41235570 Msa1104220:CDS
TCATAAACCCTAGACCCGTT+CGG 0.553729 7_3:-41235616 None:intergenic
GCTTTCATTTCATCAATGTG+AGG 0.554721 7_3:+41236397 Msa1104220:three_prime_UTR
ACCAAACCCTCTCCGACCAA+TGG 0.556784 7_3:+41234873 Msa1104220:CDS
ACTTGTGATCCGGTTCAACC+CGG 0.561213 7_3:+41235802 Msa1104220:CDS
AAGATAAAGTAGGAGATGGA+TGG 0.562826 7_3:-41234292 None:intergenic
TTTGGTGTGTGGCGGTGCGT+GGG 0.564836 7_3:+41235722 Msa1104220:CDS
GTTGGATTTGATCCATTGGT+CGG 0.568173 7_3:-41234885 None:intergenic
GTTTGATTCTACGCATCGCA+GGG 0.568728 7_3:-41235003 None:intergenic
AAAACCGGTTGTTGGGACAC+CGG 0.571712 7_3:+41235495 Msa1104220:CDS
CAAGTGTGACACTTTGAAGG+AGG 0.572437 7_3:+41236281 Msa1104220:exon
GTTGAACCGGATCACAAGTC+CGG 0.573766 7_3:-41235798 None:intergenic
GTATAAACCAAACCCGAACC+CGG 0.574382 7_3:-41235292 None:intergenic
AAGGTTGAATGTTACTAGAG+GGG 0.577100 7_3:-41236423 None:intergenic
CACAACACAACCCATTTCCA+GGG 0.581424 7_3:+41234268 Msa1104220:five_prime_UTR
GAATTTATTTGATCAGATGG+TGG 0.583307 7_3:+41235315 Msa1104220:CDS
TTCGGGGAAGACACCGGAGT+CGG 0.590570 7_3:+41235648 Msa1104220:CDS
TAGCCAATCCAAATGAATCC+AGG 0.590664 7_3:-41236639 None:intergenic
TTAATGAATGATTCAAATGG+AGG 0.591003 7_3:-41236316 None:intergenic
AGTAGGAGATGGATGGACCC+TGG 0.592390 7_3:-41234285 None:intergenic
TGATCTTTCGGGGAAGACAC+CGG 0.599336 7_3:+41235642 Msa1104220:CDS
AGGTTGAATGTTACTAGAGG+GGG 0.602309 7_3:-41236422 None:intergenic
CTTGTGATCCGGTTCAACCC+GGG 0.606763 7_3:+41235803 Msa1104220:CDS
ATTTGATCCATTGGTCGGAG+AGG 0.616370 7_3:-41234880 None:intergenic
TATGGCTCATTATTCTCCGG+TGG 0.617117 7_3:-41235688 None:intergenic
TGATTTCGTTTCCTACGAGG+AGG 0.619686 7_3:-41234943 None:intergenic
GAGCAATGTGACAGCGTTAG+GGG 0.621930 7_3:+41235744 Msa1104220:CDS
CAAATTAACATTAACCGGGT+CGG 0.622515 7_3:-41235227 None:intergenic
TTTAATGAGAATATTAAACC+GGG 0.624692 7_3:+41235559 Msa1104220:CDS
TGAAGGTTGAATGTTACTAG+AGG 0.624775 7_3:-41236425 None:intergenic
TCACAACCAGGAATCTGCTA+CGG 0.630316 7_3:+41234674 Msa1104220:CDS
TAAAGGAAAGATTTGGTGTG+TGG 0.635044 7_3:+41235711 Msa1104220:CDS
GAAACGGGTTGGCCTAATGG+TGG 0.635140 7_3:+41235397 Msa1104220:CDS
GCTTGCTACTCAAACTGCAA+AGG 0.636317 7_3:+41235930 Msa1104220:CDS
CAATGTGACAGCGTTAGGGG+AGG 0.639465 7_3:+41235747 Msa1104220:CDS
AGATTGAGATGAGACTATGG+TGG 0.639702 7_3:-41234236 None:intergenic
TAACATTCAACCTTCAAAGG+AGG 0.643417 7_3:+41236432 Msa1104220:three_prime_UTR
AAGCCACGTGGAGTTTGGTG+TGG 0.644687 7_3:-41234972 None:intergenic
TTAATCCGTTACCTCCTCGT+AGG 0.646830 7_3:+41234932 Msa1104220:CDS
AGATGATTTCGTTTCCTACG+AGG 0.653727 7_3:-41234946 None:intergenic
TGACGTATGCTTGCTCGCAG+GGG 0.654371 7_3:+41235773 Msa1104220:CDS
TTAACCGGGTCGGAGCTCCA+AGG 0.655245 7_3:-41235217 None:intergenic
TGCGTTTCGTAAAGATATAG+CGG 0.657744 7_3:+41235117 Msa1104220:CDS
AATCCAAATTAACATTAACC+GGG 0.666875 7_3:-41235231 None:intergenic
GGATGTTCGGATATTTATCG+CGG 0.670356 7_3:+41235375 Msa1104220:CDS
TTTATGAGATTGATCTTTCG+GGG 0.672089 7_3:+41235632 Msa1104220:CDS
AACCGGGTCCAGGTACGGAG+CGG 0.677668 7_3:+41235575 Msa1104220:CDS
CATCCACACCAAACTCCACG+TGG 0.678852 7_3:+41234969 Msa1104220:CDS
GCACAGTTTAAGAAGACTGG+AGG 0.681953 7_3:+41235889 Msa1104220:CDS
AGGAAAGATTTGGTGTGTGG+CGG 0.713466 7_3:+41235714 Msa1104220:CDS
AAACTCGGAAGAATTGAACC+CGG 0.715518 7_3:-41235523 None:intergenic
AGGGACGAGATGAAGCCACG+TGG 0.766058 7_3:-41234984 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAGTTGTTTATTTTTTT+GGG - chr7_3:41234326-41234345 None:intergenic 10.0%
!!! TAATAGAAATTTTGTTAAAA+AGG + chr7_3:41235465-41235484 Msa1104220:CDS 10.0%
!! GAAAAAATTTGTAAATAAAG+AGG - chr7_3:41234114-41234133 None:intergenic 15.0%
!!! TAATTGACTCTTTTTTTAAT+TGG - chr7_3:41234144-41234163 None:intergenic 15.0%
!!! AAAAGTTGTTTATTTTTTTG+GGG - chr7_3:41234325-41234344 None:intergenic 15.0%
!!! GAAAAAGTTGTTTATTTTTT+TGG - chr7_3:41234327-41234346 None:intergenic 15.0%
!! AACAAAATTTCTATTATAAG+TGG - chr7_3:41235461-41235480 None:intergenic 15.0%
!! AATAAAAAAATGTACTTACT+TGG - chr7_3:41235958-41235977 None:intergenic 15.0%
!! AAAATAAAAACGTTAAGTAA+TGG - chr7_3:41236218-41236237 None:intergenic 15.0%
!! TATAAGAGGAAATTTAAATT+TGG + chr7_3:41236349-41236368 Msa1104220:three_prime_UTR 15.0%
!! AAAAACAGCAAAAACAAAAA+TGG + chr7_3:41234453-41234472 Msa1104220:exon 20.0%
!! TTCTTATTAATATACTTCAC+AGG + chr7_3:41234635-41234654 Msa1104220:intron 20.0%
!! AAAAGAAAAAAGATTTAGTC+CGG - chr7_3:41235177-41235196 None:intergenic 20.0%
!!! GACTAAATCTTTTTTCTTTT+TGG + chr7_3:41235177-41235196 Msa1104220:CDS 20.0%
!! TAATCCAAATTAACATTAAC+CGG - chr7_3:41235235-41235254 None:intergenic 20.0%
!! TAAAAAGGTTATGAAAAAAC+CGG + chr7_3:41235480-41235499 Msa1104220:CDS 20.0%
!! AACAAAGCAAATAAAAAACT+CGG - chr7_3:41235541-41235560 None:intergenic 20.0%
!! GTTTAATGAGAATATTAAAC+CGG + chr7_3:41235558-41235577 Msa1104220:CDS 20.0%
!! TTTAATGAGAATATTAAACC+GGG + chr7_3:41235559-41235578 Msa1104220:CDS 20.0%
!! TAATACTAAACAATTCACAA+TGG + chr7_3:41235979-41235998 Msa1104220:intron 20.0%
!! CAATTTAATGGTTATACATT+TGG - chr7_3:41236063-41236082 None:intergenic 20.0%
!! AATCAAGTACTTCAATTTAA+TGG - chr7_3:41236075-41236094 None:intergenic 20.0%
!! GTAGTTAAGTTTAAATTAAC+TGG - chr7_3:41236116-41236135 None:intergenic 20.0%
!!! AGTAATGGAAAAGTATAATA+AGG - chr7_3:41236203-41236222 None:intergenic 20.0%
!! CAATTAATGAATGATTCAAA+TGG - chr7_3:41236322-41236341 None:intergenic 20.0%
!! TTAATTGACATTTCTATAAG+AGG + chr7_3:41236335-41236354 Msa1104220:three_prime_UTR 20.0%
! ATAAAAGATAAAGTAGGAGA+TGG - chr7_3:41234299-41234318 None:intergenic 25.0%
!! CTACTTTCTATTTTCAATGT+TGG - chr7_3:41234377-41234396 None:intergenic 25.0%
!!! TTGTGTTTTTTAAGGTTGTT+AGG - chr7_3:41234799-41234818 None:intergenic 25.0%
!!! TTGGATGTTTGTGTTTTTTA+AGG - chr7_3:41234807-41234826 None:intergenic 25.0%
! AGAATCAAAAGAATCAAAGT+TGG + chr7_3:41235043-41235062 Msa1104220:CDS 25.0%
! AATCCAAATTAACATTAACC+GGG - chr7_3:41235234-41235253 None:intergenic 25.0%
! TACGAATTTATTTGATCAGA+TGG + chr7_3:41235312-41235331 Msa1104220:CDS 25.0%
!!! AAGTACTTGATTTGTTAACA+AGG + chr7_3:41236083-41236102 Msa1104220:intron 25.0%
!!! ATCTTTTTTTGAACATGTGT+AGG + chr7_3:41236238-41236257 Msa1104220:intron 25.0%
! TTAATGAATGATTCAAATGG+AGG - chr7_3:41236319-41236338 None:intergenic 25.0%
!! ATGAATGATTTTCATAGCAA+TGG + chr7_3:41236466-41236485 Msa1104220:three_prime_UTR 25.0%
! AGCATCATGATTTCAAATAA+TGG - chr7_3:41236552-41236571 None:intergenic 25.0%
GGGAAGATAAAAGATAAAGT+AGG - chr7_3:41234305-41234324 None:intergenic 30.0%
!!! CTATTTTCAATGTTGGTGAT+GGG - chr7_3:41234370-41234389 None:intergenic 30.0%
!! TCTATTTTCAATGTTGGTGA+TGG - chr7_3:41234371-41234390 None:intergenic 30.0%
TGAAATGTTGTTGAGTAGAA+TGG - chr7_3:41234503-41234522 None:intergenic 30.0%
! ATTAAGGAGAGTGATTTTGA+GGG - chr7_3:41234725-41234744 None:intergenic 30.0%
TGCGTTTAAGGATTTGATTA+AGG - chr7_3:41234741-41234760 None:intergenic 30.0%
TTAAGGTTGTTAGGATTTGT+GGG - chr7_3:41234790-41234809 None:intergenic 30.0%
! TTTAAGGTTGTTAGGATTTG+TGG - chr7_3:41234791-41234810 None:intergenic 30.0%
ATTAAGGTTTGTGGGTAATA+AGG - chr7_3:41234917-41234936 None:intergenic 30.0%
!!! ATGTTTATCCGTTTTTTCCT+TGG + chr7_3:41235200-41235219 Msa1104220:CDS 30.0%
GTTTAGAGAGAGTAATGTTA+CGG + chr7_3:41235261-41235280 Msa1104220:CDS 30.0%
GAATTTATTTGATCAGATGG+TGG + chr7_3:41235315-41235334 Msa1104220:CDS 30.0%
TTATGAAAAAACCGGTTGTT+GGG + chr7_3:41235488-41235507 Msa1104220:CDS 30.0%
GGTTTATGAGATTGATCTTT+CGG + chr7_3:41235630-41235649 Msa1104220:CDS 30.0%
GTTTATGAGATTGATCTTTC+GGG + chr7_3:41235631-41235650 Msa1104220:CDS 30.0%
TTTATGAGATTGATCTTTCG+GGG + chr7_3:41235632-41235651 Msa1104220:CDS 30.0%
TTATATGGCTCATTATTCTC+CGG - chr7_3:41235694-41235713 None:intergenic 30.0%
GAGAATAATGAGCCATATAA+AGG + chr7_3:41235694-41235713 Msa1104220:CDS 30.0%
AGCCATATAAAGGAAAGATT+TGG + chr7_3:41235704-41235723 Msa1104220:CDS 30.0%
CACCAAATCTTTCCTTTATA+TGG - chr7_3:41235709-41235728 None:intergenic 30.0%
!! GAGGAACTTGTTACTTTAAT+GGG + chr7_3:41235908-41235927 Msa1104220:CDS 30.0%
! TTTTGAACATGTGTAGGTTA+TGG + chr7_3:41236244-41236263 Msa1104220:intron 30.0%
TAGTAACATTCAACCTTCAA+AGG + chr7_3:41236429-41236448 Msa1104220:three_prime_UTR 30.0%
! ATGATTTTCATAGCAATGGT+AGG + chr7_3:41236470-41236489 Msa1104220:three_prime_UTR 30.0%
! TGATTTTCATAGCAATGGTA+GGG + chr7_3:41236471-41236490 Msa1104220:three_prime_UTR 30.0%
TATTCATTTATTGCAACACC+TGG + chr7_3:41236621-41236640 Msa1104220:three_prime_UTR 30.0%
CTATGGTGGTTTGATAAAGT+GGG - chr7_3:41234225-41234244 None:intergenic 35.0%
ACTATGGTGGTTTGATAAAG+TGG - chr7_3:41234226-41234245 None:intergenic 35.0%
AGAAGATTGAGATGAGACTA+TGG - chr7_3:41234242-41234261 None:intergenic 35.0%
AAGATAAAGTAGGAGATGGA+TGG - chr7_3:41234295-41234314 None:intergenic 35.0%
TTTCTTCAATCTCATCTCTC+TGG + chr7_3:41234547-41234566 Msa1104220:intron 35.0%
TTCTTCAATCTCATCTCTCT+GGG + chr7_3:41234548-41234567 Msa1104220:intron 35.0%
! GATTAAGGAGAGTGATTTTG+AGG - chr7_3:41234726-41234745 None:intergenic 35.0%
TGTTTGTGATGAGTTCGTTT+GGG - chr7_3:41234844-41234863 None:intergenic 35.0%
ATGTTTGTGATGAGTTCGTT+TGG - chr7_3:41234845-41234864 None:intergenic 35.0%
TGCGTTTCGTAAAGATATAG+CGG + chr7_3:41235117-41235136 Msa1104220:CDS 35.0%
AAGTTATTAAGCCCATGCTT+AGG + chr7_3:41235143-41235162 Msa1104220:CDS 35.0%
CGGTTTAAAAACCTAAGCAT+GGG - chr7_3:41235157-41235176 None:intergenic 35.0%
! CCATGCTTAGGTTTTTAAAC+CGG + chr7_3:41235155-41235174 Msa1104220:CDS 35.0%
CAAATTAACATTAACCGGGT+CGG - chr7_3:41235230-41235249 None:intergenic 35.0%
AGAGAGTAATGTTACGGTTA+AGG + chr7_3:41235267-41235286 Msa1104220:CDS 35.0%
TAATGTTACGGTTAAGGATC+CGG + chr7_3:41235273-41235292 Msa1104220:CDS 35.0%
! TATTTTGCGATGGAGAAACT+TGG + chr7_3:41235346-41235365 Msa1104220:CDS 35.0%
! ATTTTGCGATGGAGAAACTT+GGG + chr7_3:41235347-41235366 Msa1104220:CDS 35.0%
GTTATGAAAAAACCGGTTGT+TGG + chr7_3:41235487-41235506 Msa1104220:CDS 35.0%
!! TAAAGGAAAGATTTGGTGTG+TGG + chr7_3:41235711-41235730 Msa1104220:CDS 35.0%
!! AAAGTGTTTTAAGCCCGAAT+CGG + chr7_3:41235828-41235847 Msa1104220:CDS 35.0%
! CTATGCGTTTAGTTCCTATT+GGG + chr7_3:41235867-41235886 Msa1104220:CDS 35.0%
!! GGAGGAACTTGTTACTTTAA+TGG + chr7_3:41235907-41235926 Msa1104220:CDS 35.0%
GCTTTCATTTCATCAATGTG+AGG + chr7_3:41236397-41236416 Msa1104220:three_prime_UTR 35.0%
AAGGTTGAATGTTACTAGAG+GGG - chr7_3:41236426-41236445 None:intergenic 35.0%
GAAGGTTGAATGTTACTAGA+GGG - chr7_3:41236427-41236446 None:intergenic 35.0%
TGAAGGTTGAATGTTACTAG+AGG - chr7_3:41236428-41236447 None:intergenic 35.0%
TAACATTCAACCTTCAAAGG+AGG + chr7_3:41236432-41236451 Msa1104220:three_prime_UTR 35.0%
AATGTAAGTTCCTCCTTTGA+AGG - chr7_3:41236445-41236464 None:intergenic 35.0%
AGATTGAGATGAGACTATGG+TGG - chr7_3:41234239-41234258 None:intergenic 40.0%
CTTTCAGAAGTTGTGCTATG+TGG - chr7_3:41234424-41234443 None:intergenic 40.0%
GTAGAATGGAGAAGAGAAAG+AGG - chr7_3:41234489-41234508 None:intergenic 40.0%
!!! TCAGCTTTTTCATCACAACC+AGG + chr7_3:41234662-41234681 Msa1104220:CDS 40.0%
!! GTGATTTTGAGGGTTTTGGT+AGG - chr7_3:41234715-41234734 None:intergenic 40.0%
!! GAGAGTGATTTTGAGGGTTT+TGG - chr7_3:41234719-41234738 None:intergenic 40.0%
TTTGATGCGCTTTGCGTTTA+AGG - chr7_3:41234753-41234772 None:intergenic 40.0%
! TTGGGAGCATGATTGAGATT+TGG - chr7_3:41234826-41234845 None:intergenic 40.0%
GTTGGATTTGATCCATTGGT+CGG - chr7_3:41234888-41234907 None:intergenic 40.0%
GAACGTTGGATTTGATCCAT+TGG - chr7_3:41234892-41234911 None:intergenic 40.0%
TGGGTAATAAGGTAGAACGT+TGG - chr7_3:41234906-41234925 None:intergenic 40.0%
!! GGTAACGGATTAAGGTTTGT+GGG - chr7_3:41234925-41234944 None:intergenic 40.0%
!! AGGTAACGGATTAAGGTTTG+TGG - chr7_3:41234926-41234945 None:intergenic 40.0%
AGATGATTTCGTTTCCTACG+AGG - chr7_3:41234949-41234968 None:intergenic 40.0%
! AGTTGGTACTTCTTCTGCTA+TGG + chr7_3:41235060-41235079 Msa1104220:CDS 40.0%
CCGGTTTAAAAACCTAAGCA+TGG - chr7_3:41235158-41235177 None:intergenic 40.0%
CGACCCGGTTAATGTTAATT+TGG + chr7_3:41235228-41235247 Msa1104220:CDS 40.0%
AATGTTACGGTTAAGGATCC+GGG + chr7_3:41235274-41235293 Msa1104220:CDS 40.0%
!! ATTTGATCAGATGGTGGATG+CGG + chr7_3:41235321-41235340 Msa1104220:CDS 40.0%
GCGATAAATATCCGAACATC+CGG - chr7_3:41235376-41235395 None:intergenic 40.0%
GGATGTTCGGATATTTATCG+CGG + chr7_3:41235375-41235394 Msa1104220:CDS 40.0%
TCGGATATTTATCGCGGAAA+CGG + chr7_3:41235381-41235400 Msa1104220:CDS 40.0%
TGATCAACATCACCACCATT+AGG - chr7_3:41235412-41235431 None:intergenic 40.0%
! GGTGGTGATGTTGATCAAAT+TGG + chr7_3:41235415-41235434 Msa1104220:CDS 40.0%
AAACTCGGAAGAATTGAACC+CGG - chr7_3:41235526-41235545 None:intergenic 40.0%
!! TTTGGGTTGTTGTATCCGAA+CGG + chr7_3:41235601-41235620 Msa1104220:CDS 40.0%
!!! GGCTTAAAACACTTTTTCCC+GGG - chr7_3:41235823-41235842 None:intergenic 40.0%
!!! GGGCTTAAAACACTTTTTCC+CGG - chr7_3:41235824-41235843 None:intergenic 40.0%
!!! TTAAGCCCGAATCGGTTTTT+TGG + chr7_3:41235836-41235855 Msa1104220:CDS 40.0%
AGCATGCCAAAAAACCGATT+CGG - chr7_3:41235845-41235864 None:intergenic 40.0%
! GCTATGCGTTTAGTTCCTAT+TGG + chr7_3:41235866-41235885 Msa1104220:CDS 40.0%
CTTCTTAAACTGTGCCCAAT+AGG - chr7_3:41235884-41235903 None:intergenic 40.0%
TTCCAAGTGTGACACTTTGA+AGG + chr7_3:41236278-41236297 Msa1104220:CDS 40.0%
AGGTTGAATGTTACTAGAGG+GGG - chr7_3:41236425-41236444 None:intergenic 40.0%
TTGCAACACCTGGATTCATT+TGG + chr7_3:41236631-41236650 Msa1104220:three_prime_UTR 40.0%
ACACAACACAACCCATTTCC+AGG + chr7_3:41234267-41234286 Msa1104220:five_prime_UTR 45.0%
CACAACACAACCCATTTCCA+GGG + chr7_3:41234268-41234287 Msa1104220:five_prime_UTR 45.0%
TCACAACCAGGAATCTGCTA+CGG + chr7_3:41234674-41234693 Msa1104220:CDS 45.0%
TTTGATCCATTGGTCGGAGA+GGG - chr7_3:41234882-41234901 None:intergenic 45.0%
ATTTGATCCATTGGTCGGAG+AGG - chr7_3:41234883-41234902 None:intergenic 45.0%
!! TACGAGGAGGTAACGGATTA+AGG - chr7_3:41234933-41234952 None:intergenic 45.0%
TTAATCCGTTACCTCCTCGT+AGG + chr7_3:41234932-41234951 Msa1104220:CDS 45.0%
TGATTTCGTTTCCTACGAGG+AGG - chr7_3:41234946-41234965 None:intergenic 45.0%
GTTTGATTCTACGCATCGCA+GGG - chr7_3:41235006-41235025 None:intergenic 45.0%
TGTTTGATTCTACGCATCGC+AGG - chr7_3:41235007-41235026 None:intergenic 45.0%
TTACGAAACGCAGCGTTTGA+AGG - chr7_3:41235109-41235128 None:intergenic 45.0%
TCGGAGCTCCAAGGAAAAAA+CGG - chr7_3:41235211-41235230 None:intergenic 45.0%
GTATAAACCAAACCCGAACC+CGG - chr7_3:41235295-41235314 None:intergenic 45.0%
!!! GGATGCGGTTTATTTTGCGA+TGG + chr7_3:41235336-41235355 Msa1104220:CDS 45.0%
GATGGAGAAACTTGGGTTTC+CGG + chr7_3:41235354-41235373 Msa1104220:CDS 45.0%
AACTTGGGTTTCCGGATGTT+CGG + chr7_3:41235362-41235381 Msa1104220:CDS 45.0%
CGGATATTTATCGCGGAAAC+GGG + chr7_3:41235382-41235401 Msa1104220:CDS 45.0%
TATTTATCGCGGAAACGGGT+TGG + chr7_3:41235386-41235405 Msa1104220:CDS 45.0%
GAGAATATTAAACCGGGTCC+AGG + chr7_3:41235565-41235584 Msa1104220:CDS 45.0%
TATTAAACCGGGTCCAGGTA+CGG + chr7_3:41235570-41235589 Msa1104220:CDS 45.0%
! TTGGGTTGTTGTATCCGAAC+GGG + chr7_3:41235602-41235621 Msa1104220:CDS 45.0%
TCATAAACCCTAGACCCGTT+CGG - chr7_3:41235619-41235638 None:intergenic 45.0%
TATGGCTCATTATTCTCCGG+TGG - chr7_3:41235691-41235710 None:intergenic 45.0%
!! AGGAAAGATTTGGTGTGTGG+CGG + chr7_3:41235714-41235733 Msa1104220:CDS 45.0%
! GCATGCCAAAAAACCGATTC+GGG - chr7_3:41235844-41235863 None:intergenic 45.0%
TGGGCACAGTTTAAGAAGAC+TGG + chr7_3:41235886-41235905 Msa1104220:CDS 45.0%
GCACAGTTTAAGAAGACTGG+AGG + chr7_3:41235889-41235908 Msa1104220:CDS 45.0%
! GCTTGCTACTCAAACTGCAA+AGG + chr7_3:41235930-41235949 Msa1104220:CDS 45.0%
! CTCCTTCAAAGTGTCACACT+TGG - chr7_3:41236283-41236302 None:intergenic 45.0%
! CAAGTGTGACACTTTGAAGG+AGG + chr7_3:41236281-41236300 Msa1104220:exon 45.0%
GATGGATGGACCCTGGAAAT+GGG - chr7_3:41234281-41234300 None:intergenic 50.0%
AGATGGATGGACCCTGGAAA+TGG - chr7_3:41234282-41234301 None:intergenic 50.0%
AGTTGACCGTAGCAGATTCC+TGG - chr7_3:41234683-41234702 None:intergenic 50.0%
GGAATCTGCTACGGTCAACT+AGG + chr7_3:41234683-41234702 Msa1104220:CDS 50.0%
CGTTTCCTACGAGGAGGTAA+CGG - chr7_3:41234940-41234959 None:intergenic 50.0%
AGATGAAGCCACGTGGAGTT+TGG - chr7_3:41234980-41234999 None:intergenic 50.0%
TACGGTTAAGGATCCGGGTT+CGG + chr7_3:41235279-41235298 Msa1104220:CDS 50.0%
AAAACCGGTTGTTGGGACAC+CGG + chr7_3:41235495-41235514 Msa1104220:CDS 50.0%
AGAATTGAACCCGGTCTAGC+CGG - chr7_3:41235517-41235536 None:intergenic 50.0%
TGTTGTATCCGAACGGGTCT+AGG + chr7_3:41235608-41235627 Msa1104220:CDS 50.0%
GTTGTATCCGAACGGGTCTA+GGG + chr7_3:41235609-41235628 Msa1104220:CDS 50.0%
TGATCTTTCGGGGAAGACAC+CGG + chr7_3:41235642-41235661 Msa1104220:CDS 50.0%
GGAGCAATGTGACAGCGTTA+GGG + chr7_3:41235743-41235762 Msa1104220:CDS 50.0%
GAGCAATGTGACAGCGTTAG+GGG + chr7_3:41235744-41235763 Msa1104220:CDS 50.0%
TTGACGTATGCTTGCTCGCA+GGG + chr7_3:41235772-41235791 Msa1104220:CDS 50.0%
GTTGAACCGGATCACAAGTC+CGG - chr7_3:41235801-41235820 None:intergenic 50.0%
ACTTGTGATCCGGTTCAACC+CGG + chr7_3:41235802-41235821 Msa1104220:CDS 50.0%
!!! CACTTTTTCCCGGGTTGAAC+CGG - chr7_3:41235814-41235833 None:intergenic 50.0%
AGTAGGAGATGGATGGACCC+TGG - chr7_3:41234288-41234307 None:intergenic 55.0%
ACCAAACCCTCTCCGACCAA+TGG + chr7_3:41234873-41234892 Msa1104220:CDS 55.0%
TCCATTGGTCGGAGAGGGTT+TGG - chr7_3:41234877-41234896 None:intergenic 55.0%
CATCCACACCAAACTCCACG+TGG + chr7_3:41234969-41234988 Msa1104220:CDS 55.0%
! AAGCCACGTGGAGTTTGGTG+TGG - chr7_3:41234975-41234994 None:intergenic 55.0%
CGAAACGCAGCGTTTGAAGG+TGG - chr7_3:41235106-41235125 None:intergenic 55.0%
! TTTTCCTTGGAGCTCCGACC+CGG + chr7_3:41235213-41235232 Msa1104220:CDS 55.0%
ACGGTTAAGGATCCGGGTTC+GGG + chr7_3:41235280-41235299 Msa1104220:CDS 55.0%
! TAAGGATCCGGGTTCGGGTT+TGG + chr7_3:41235285-41235304 Msa1104220:CDS 55.0%
!! GAAACGGGTTGGCCTAATGG+TGG + chr7_3:41235397-41235416 Msa1104220:CDS 55.0%
! CAGGTACGGAGCGGCATTTT+GGG + chr7_3:41235584-41235603 Msa1104220:CDS 55.0%
! AGTGCTGGAAACTCCGACTC+CGG - chr7_3:41235664-41235683 None:intergenic 55.0%
GTTTCCAGCACTTCCTCCAC+CGG + chr7_3:41235672-41235691 Msa1104220:CDS 55.0%
GGCTCATTATTCTCCGGTGG+AGG - chr7_3:41235688-41235707 None:intergenic 55.0%
GGGAGCAATGTGACAGCGTT+AGG + chr7_3:41235742-41235761 Msa1104220:CDS 55.0%
CAATGTGACAGCGTTAGGGG+AGG + chr7_3:41235747-41235766 Msa1104220:CDS 55.0%
GTTGACGTATGCTTGCTCGC+AGG + chr7_3:41235771-41235790 Msa1104220:CDS 55.0%
TGACGTATGCTTGCTCGCAG+GGG + chr7_3:41235773-41235792 Msa1104220:CDS 55.0%
CTTGTGATCCGGTTCAACCC+GGG + chr7_3:41235803-41235822 Msa1104220:CDS 55.0%
AGGGACGAGATGAAGCCACG+TGG - chr7_3:41234987-41235006 None:intergenic 60.0%
TTAACCGGGTCGGAGCTCCA+AGG - chr7_3:41235220-41235239 None:intergenic 60.0%
!! GCGGAAACGGGTTGGCCTAA+TGG + chr7_3:41235394-41235413 Msa1104220:CDS 60.0%
CTAGCCGGTGTCCCAACAAC+CGG - chr7_3:41235502-41235521 None:intergenic 60.0%
! TGTTGGGACACCGGCTAGAC+CGG + chr7_3:41235504-41235523 Msa1104220:CDS 60.0%
CCAAAATGCCGCTCCGTACC+TGG - chr7_3:41235586-41235605 None:intergenic 60.0%
! CCAGGTACGGAGCGGCATTT+TGG + chr7_3:41235583-41235602 Msa1104220:CDS 60.0%
TTCGGGGAAGACACCGGAGT+CGG + chr7_3:41235648-41235667 Msa1104220:CDS 60.0%
! TTCTCCGGTGGAGGAAGTGC+TGG - chr7_3:41235679-41235698 None:intergenic 60.0%
!! ATTTGGTGTGTGGCGGTGCG+TGG + chr7_3:41235721-41235740 Msa1104220:CDS 60.0%
! TTTGGTGTGTGGCGGTGCGT+GGG + chr7_3:41235722-41235741 Msa1104220:CDS 60.0%
ATGCTTGCTCGCAGGGGAAC+CGG + chr7_3:41235779-41235798 Msa1104220:CDS 60.0%
GGGGAACCGGACTTGTGATC+CGG + chr7_3:41235792-41235811 Msa1104220:CDS 60.0%
! GTTGGGACACCGGCTAGACC+GGG + chr7_3:41235505-41235524 Msa1104220:CDS 65.0%
AACCGGGTCCAGGTACGGAG+CGG + chr7_3:41235575-41235594 Msa1104220:CDS 65.0%
TGCCGCTCCGTACCTGGACC+CGG - chr7_3:41235580-41235599 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 41234106 41236658 41234106 ID=Msa1104220;Name=Msa1104220
chr7_3 mRNA 41234106 41236658 41234106 ID=Msa1104220-mRNA-1;Parent=Msa1104220;Name=Msa1104220-mRNA-1;_AED=0.01;_eAED=0.01;_QI=366|1|1|1|1|0.66|3|361|463
chr7_3 exon 41234106 41234523 41234106 ID=Msa1104220-mRNA-1:exon:8369;Parent=Msa1104220-mRNA-1
chr7_3 exon 41234657 41235958 41234657 ID=Msa1104220-mRNA-1:exon:8370;Parent=Msa1104220-mRNA-1
chr7_3 exon 41236260 41236658 41236260 ID=Msa1104220-mRNA-1:exon:8371;Parent=Msa1104220-mRNA-1
chr7_3 five_prime_UTR 41234106 41234471 41234106 ID=Msa1104220-mRNA-1:five_prime_utr;Parent=Msa1104220-mRNA-1
chr7_3 CDS 41234472 41234523 41234472 ID=Msa1104220-mRNA-1:cds;Parent=Msa1104220-mRNA-1
chr7_3 CDS 41234657 41235958 41234657 ID=Msa1104220-mRNA-1:cds;Parent=Msa1104220-mRNA-1
chr7_3 CDS 41236260 41236297 41236260 ID=Msa1104220-mRNA-1:cds;Parent=Msa1104220-mRNA-1
chr7_3 three_prime_UTR 41236298 41236658 41236298 ID=Msa1104220-mRNA-1:three_prime_utr;Parent=Msa1104220-mRNA-1
Gene Sequence

>Msa1104220

ATGGCTTTGTTTCTCCTCTTTCTCTTCTCCATTCTACTCAACAACATTTCAAGTAGCTCAGCTTTTTCATCACAACCAGGAATCTGCTACGGTCAACTAGGAAACAACCTACCAAAACCCTCAAAATCACTCTCCTTAATCAAATCCTTAAACGCAAAGCGCATCAAAATCTATGACACAAACCCACAAATCCTAACAACCTTAAAAAACACAAACATCCAAATCTCAATCATGCTCCCAAACGAACTCATCACAAACATCTCATCAAACCAAACCCTCTCCGACCAATGGATCAAATCCAACGTTCTACCTTATTACCCACAAACCTTAATCCGTTACCTCCTCGTAGGAAACGAAATCATCTCATCCACACCAAACTCCACGTGGCTTCATCTCGTCCCTGCGATGCGTAGAATCAAACACTCTTTGAAAAAATTCAGAATCAAAAGAATCAAAGTTGGTACTTCTTCTGCTATGGATGTTCTAGAAACTTCTTTTCCACCTTCAAACGCTGCGTTTCGTAAAGATATAGCGGTTCAAGTTATTAAGCCCATGCTTAGGTTTTTAAACCGGACTAAATCTTTTTTCTTTTTGGATGTTTATCCGTTTTTTCCTTGGAGCTCCGACCCGGTTAATGTTAATTTGGATTATGCTTTGTTTAGAGAGAGTAATGTTACGGTTAAGGATCCGGGTTCGGGTTTGGTTTATACGAATTTATTTGATCAGATGGTGGATGCGGTTTATTTTGCGATGGAGAAACTTGGGTTTCCGGATGTTCGGATATTTATCGCGGAAACGGGTTGGCCTAATGGTGGTGATGTTGATCAAATTGGTGCTAATGTTTTTAATGCTGCCACTTATAATAGAAATTTTGTTAAAAAGGTTATGAAAAAACCGGTTGTTGGGACACCGGCTAGACCGGGTTCAATTCTTCCGAGTTTTTTATTTGCTTTGTTTAATGAGAATATTAAACCGGGTCCAGGTACGGAGCGGCATTTTGGGTTGTTGTATCCGAACGGGTCTAGGGTTTATGAGATTGATCTTTCGGGGAAGACACCGGAGTCGGAGTTTCCAGCACTTCCTCCACCGGAGAATAATGAGCCATATAAAGGAAAGATTTGGTGTGTGGCGGTGCGTGGGAGCAATGTGACAGCGTTAGGGGAGGCGTTGACGTATGCTTGCTCGCAGGGGAACCGGACTTGTGATCCGGTTCAACCCGGGAAAAAGTGTTTTAAGCCCGAATCGGTTTTTTGGCATGCTAGCTATGCGTTTAGTTCCTATTGGGCACAGTTTAAGAAGACTGGAGGAACTTGTTACTTTAATGGGCTTGCTACTCAAACTGCAAAGGATCCAAGTTATGGATCTTGCAAGTTTCCAAGTGTGACACTTTGA
Protein sequence

>Msa1104220

MALFLLFLFSILLNNISSSSAFSSQPGICYGQLGNNLPKPSKSLSLIKSLNAKRIKIYDTNPQILTTLKNTNIQISIMLPNELITNISSNQTLSDQWIKSNVLPYYPQTLIRYLLVGNEIISSTPNSTWLHLVPAMRRIKHSLKKFRIKRIKVGTSSAMDVLETSFPPSNAAFRKDIAVQVIKPMLRFLNRTKSFFFLDVYPFFPWSSDPVNVNLDYALFRESNVTVKDPGSGLVYTNLFDQMVDAVYFAMEKLGFPDVRIFIAETGWPNGGDVDQIGANVFNAATYNRNFVKKVMKKPVVGTPARPGSILPSFLFALFNENIKPGPGTERHFGLLYPNGSRVYEIDLSGKTPESEFPALPPPENNEPYKGKIWCVAVRGSNVTALGEALTYACSQGNRTCDPVQPGKKCFKPESVFWHASYAFSSYWAQFKKTGGTCYFNGLATQTAKDPSYGSCKFPSVTL*