AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1218550


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1218550 MtrunA17_Chr4g0068141 95.455 176 8 0 1 176 201 376 5.57e-126 358
Msa1218550 MtrunA17_Chr4g0068121 77.714 175 39 0 1 175 201 375 7.76e-103 300
Msa1218550 MtrunA17_Chr4g0036961 78.409 176 38 0 1 176 201 376 2.46e-102 299
Msa1218550 MtrunA17_Chr3g0082001 40.805 174 87 3 7 172 225 390 3.39e-42 145
Msa1218550 MtrunA17_Chr3g0134551 40.805 174 87 3 7 172 225 390 3.69e-42 145
Msa1218550 MtrunA17_Chr1g0152791 39.766 171 102 1 2 172 38 207 9.88e-42 139
Msa1218550 MtrunA17_Chr3g0134561 40.116 172 101 2 2 172 220 390 7.38e-41 141
Msa1218550 MtrunA17_Chr3g0134571 39.759 166 99 1 7 172 214 378 9.10e-40 138
Msa1218550 MtrunA17_Chr1g0152811 39.181 171 103 1 2 172 256 425 2.22e-39 138
Msa1218550 MtrunA17_Chr1g0152831 41.212 165 96 1 2 166 254 417 7.31e-39 138
Msa1218550 MtrunA17_Chr3g0104611 35.429 175 107 3 3 172 204 377 5.89e-30 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1218550 AT4G24220.1 35.673 171 109 1 2 172 219 388 3.16e-36 129
Msa1218550 AT4G24220.2 35.673 171 109 1 2 172 218 387 3.96e-36 129
Msa1218550 AT5G58750.2 32.571 175 114 3 3 173 204 378 1.58e-23 95.9
Msa1218550 AT5G58750.1 32.571 175 114 3 3 173 212 386 1.69e-23 95.9

Find 34 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTAATAGTGTGAAATTC+TGG 0.190417 8_1:+81194217 Msa1218550:CDS
CGTTTCGTTATCCAGGAAAT+AGG 0.260788 8_1:+81193803 Msa1218550:CDS
TGCGTGATAAGGGTGAGATT+TGG 0.302571 8_1:+81194043 Msa1218550:CDS
GCAATTAAACGCTTGGTTCT+TGG 0.326986 8_1:-81193908 None:intergenic
GCGTGATAAGGGTGAGATTT+GGG 0.351508 8_1:+81194044 Msa1218550:CDS
TGTAGTTCAATGTTCGTAGA+TGG 0.355494 8_1:-81194271 None:intergenic
TGCTGTTAAGTTTGTGGAGT+TGG 0.382859 8_1:+81193987 Msa1218550:CDS
TGGACTTCATAAGACCAAAT+TGG 0.385145 8_1:+81194086 Msa1218550:CDS
GATCAGATTGTGGAGGAGTA+TGG 0.407176 8_1:+81194066 Msa1218550:CDS
CAAGCGTTTAATTGCACAAA+TGG 0.411281 8_1:+81193916 Msa1218550:CDS
TGAAAAGGAAGAGTTTGATT+TGG 0.422569 8_1:+81194011 Msa1218550:CDS
TCCAAAGTGTACCTATTTCC+TGG 0.427390 8_1:-81193814 None:intergenic
GGATTACCGTTTCGTTATCC+AGG 0.443194 8_1:+81193796 Msa1218550:CDS
GTTTGTGGAGTTGGATGAAA+AGG 0.451890 8_1:+81193996 Msa1218550:CDS
GGTGCAGTTTATGCGTGATA+AGG 0.456957 8_1:+81194032 Msa1218550:CDS
GTGCAGTTTATGCGTGATAA+GGG 0.465205 8_1:+81194033 Msa1218550:CDS
TAATCCTACCTGGCGACATA+TGG 0.474335 8_1:-81193779 None:intergenic
CATTTGTGCAATTAAACGCT+TGG 0.482702 8_1:-81193915 None:intergenic
TCCAGGAAATAGGTACACTT+TGG 0.499854 8_1:+81193813 Msa1218550:CDS
GTTTAATAGTGTGAAATTCT+GGG 0.501763 8_1:+81194218 Msa1218550:CDS
TGAGATTTGGGATCAGATTG+TGG 0.512590 8_1:+81194056 Msa1218550:CDS
TGCTCGCAAAGCAGCATGTG+TGG 0.516452 8_1:+81193866 Msa1218550:CDS
CTATTTCCTGGATAACGAAA+CGG 0.534217 8_1:-81193802 None:intergenic
ATGCGCTGCCATATGTCGCC+AGG 0.550376 8_1:+81193771 Msa1218550:CDS
GGAGCATTTCTGCGATATGA+CGG 0.555683 8_1:+81193834 Msa1218550:CDS
GTAGTTCAATGTTCGTAGAT+GGG 0.556971 8_1:-81194270 None:intergenic
GTGGGCTGCAGTTACAAAGA+AGG 0.580004 8_1:+81193885 Msa1218550:CDS
CACATGGAAGAGTATGTGGA+AGG 0.602569 8_1:+81193948 Msa1218550:CDS
GCTCGCAAAGCAGCATGTGT+GGG 0.604913 8_1:+81193867 Msa1218550:CDS
AACGAAACGGTAATCCTACC+TGG 0.609340 8_1:-81193789 None:intergenic
AATTCTGGGTGATGAAGCTG+AGG 0.665041 8_1:+81194232 Msa1218550:CDS
GCTGCCATATGTCGCCAGGT+AGG 0.675590 8_1:+81193775 Msa1218550:CDS
GATTTGGGATCAGATTGTGG+AGG 0.682099 8_1:+81194059 Msa1218550:CDS
ATTCTGGGTGATGAAGCTGA+GGG 0.787809 8_1:+81194233 Msa1218550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTTTAATAGTGTGAAATTCT+GGG + chr8_1:81194218-81194237 Msa1218550:CDS 25.0%
TGAAAAGGAAGAGTTTGATT+TGG + chr8_1:81194011-81194030 Msa1218550:CDS 30.0%
! ACAAGCAATTTTCTCCAATT+TGG - chr8_1:81194103-81194122 None:intergenic 30.0%
TGAAACTCAAATCGAAAAAC+TGG - chr8_1:81194141-81194160 None:intergenic 30.0%
CGTTTAATAGTGTGAAATTC+TGG + chr8_1:81194217-81194236 Msa1218550:CDS 30.0%
CTATTTCCTGGATAACGAAA+CGG - chr8_1:81193805-81193824 None:intergenic 35.0%
CATTTGTGCAATTAAACGCT+TGG - chr8_1:81193918-81193937 None:intergenic 35.0%
CAAGCGTTTAATTGCACAAA+TGG + chr8_1:81193916-81193935 Msa1218550:CDS 35.0%
!! TTTTCACATGGAAGAGTATG+TGG + chr8_1:81193944-81193963 Msa1218550:CDS 35.0%
!!! GACTTTTGCTGTTAAGTTTG+TGG + chr8_1:81193981-81194000 Msa1218550:CDS 35.0%
TGGACTTCATAAGACCAAAT+TGG + chr8_1:81194086-81194105 Msa1218550:CDS 35.0%
!!! AATTGCTTGTTTTGAAGCCT+TGG + chr8_1:81194113-81194132 Msa1218550:CDS 35.0%
CGTTTCGTTATCCAGGAAAT+AGG + chr8_1:81193803-81193822 Msa1218550:CDS 40.0%
TCCAGGAAATAGGTACACTT+TGG + chr8_1:81193813-81193832 Msa1218550:CDS 40.0%
TCCAAAGTGTACCTATTTCC+TGG - chr8_1:81193817-81193836 None:intergenic 40.0%
! GCAATTAAACGCTTGGTTCT+TGG - chr8_1:81193911-81193930 None:intergenic 40.0%
!! CAAATGGTGACGTTTTCACA+TGG + chr8_1:81193932-81193951 Msa1218550:CDS 40.0%
TGCTGTTAAGTTTGTGGAGT+TGG + chr8_1:81193987-81194006 Msa1218550:CDS 40.0%
GTTTGTGGAGTTGGATGAAA+AGG + chr8_1:81193996-81194015 Msa1218550:CDS 40.0%
GTGCAGTTTATGCGTGATAA+GGG + chr8_1:81194033-81194052 Msa1218550:CDS 40.0%
! TGAGATTTGGGATCAGATTG+TGG + chr8_1:81194056-81194075 Msa1218550:CDS 40.0%
AAATCGAAAAACTGGCACCA+AGG - chr8_1:81194133-81194152 None:intergenic 40.0%
TAATCCTACCTGGCGACATA+TGG - chr8_1:81193782-81193801 None:intergenic 45.0%
AACGAAACGGTAATCCTACC+TGG - chr8_1:81193792-81193811 None:intergenic 45.0%
GGATTACCGTTTCGTTATCC+AGG + chr8_1:81193796-81193815 Msa1218550:CDS 45.0%
! GGAGCATTTCTGCGATATGA+CGG + chr8_1:81193834-81193853 Msa1218550:CDS 45.0%
! CACATGGAAGAGTATGTGGA+AGG + chr8_1:81193948-81193967 Msa1218550:CDS 45.0%
GGTGCAGTTTATGCGTGATA+AGG + chr8_1:81194032-81194051 Msa1218550:CDS 45.0%
TGCGTGATAAGGGTGAGATT+TGG + chr8_1:81194043-81194062 Msa1218550:CDS 45.0%
GCGTGATAAGGGTGAGATTT+GGG + chr8_1:81194044-81194063 Msa1218550:CDS 45.0%
! GATTTGGGATCAGATTGTGG+AGG + chr8_1:81194059-81194078 Msa1218550:CDS 45.0%
GATCAGATTGTGGAGGAGTA+TGG + chr8_1:81194066-81194085 Msa1218550:CDS 45.0%
AATTCTGGGTGATGAAGCTG+AGG + chr8_1:81194232-81194251 Msa1218550:CDS 45.0%
ATTCTGGGTGATGAAGCTGA+GGG + chr8_1:81194233-81194252 Msa1218550:CDS 45.0%
! GTGGGCTGCAGTTACAAAGA+AGG + chr8_1:81193885-81193904 Msa1218550:CDS 50.0%
TGCTCGCAAAGCAGCATGTG+TGG + chr8_1:81193866-81193885 Msa1218550:CDS 55.0%
GCTCGCAAAGCAGCATGTGT+GGG + chr8_1:81193867-81193886 Msa1218550:CDS 55.0%
ATGCGCTGCCATATGTCGCC+AGG + chr8_1:81193771-81193790 Msa1218550:CDS 60.0%
GCTGCCATATGTCGCCAGGT+AGG + chr8_1:81193775-81193794 Msa1218550:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 81193757 81194287 81193757 ID=Msa1218550;Name=Msa1218550
chr8_1 mRNA 81193757 81194287 81193757 ID=Msa1218550-mRNA-1;Parent=Msa1218550;Name=Msa1218550-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|176
chr8_1 exon 81193757 81194287 81193757 ID=Msa1218550-mRNA-1:exon:28714;Parent=Msa1218550-mRNA-1
chr8_1 CDS 81193757 81194287 81193757 ID=Msa1218550-mRNA-1:cds;Parent=Msa1218550-mRNA-1
Gene Sequence

>Msa1218550

ATGGTACTAGCGACATGCGCTGCCATATGTCGCCAGGTAGGATTACCGTTTCGTTATCCAGGAAATAGGTACACTTTGGAGCATTTCTGCGATATGACGGATGCTCGTGTGCTCGCAAAGCAGCATGTGTGGGCTGCAGTTACAAAGAAGGCCAAGAACCAAGCGTTTAATTGCACAAATGGTGACGTTTTCACATGGAAGAGTATGTGGAAGGTGCTTTGTAAGACTTTTGCTGTTAAGTTTGTGGAGTTGGATGAAAAGGAAGAGTTTGATTTGGTGCAGTTTATGCGTGATAAGGGTGAGATTTGGGATCAGATTGTGGAGGAGTATGGACTTCATAAGACCAAATTGGAGAAAATTGCTTGTTTTGAAGCCTTGGTGCCAGTTTTTCGATTTGAGTTTCAACTTGTATCTAGTATGAATAAGAGTAAAAATTATGAGTTTTTTGAGTATGCTGAGACGTTTAATAGTGTGAAATTCTGGGTGATGAAGCTGAGGGAGATGAATCTCATACCCATCTACGAACATTGA

Protein sequence

>Msa1218550

MVLATCAAICRQVGLPFRYPGNRYTLEHFCDMTDARVLAKQHVWAAVTKKAKNQAFNCTNGDVFTWKSMWKVLCKTFAVKFVELDEKEEFDLVQFMRDKGEIWDQIVEEYGLHKTKLEKIACFEALVPVFRFEFQLVSSMNKSKNYEFFEYAETFNSVKFWVMKLREMNLIPIYEH*