AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0112570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0112570 MtrunA17_Chr1g0170651 80.141 569 46 7 2 517 8 562 0.0 863
Msa0112570 MtrunA17_Chr5g0399801 36.330 545 234 15 38 508 578 1083 7.02e-89 295
Msa0112570 MtrunA17_Chr4g0003501 35.584 548 239 17 38 511 447 954 2.93e-84 280
Msa0112570 MtrunA17_Chr4g0003511 35.219 548 243 15 38 511 542 1051 1.53e-82 277
Msa0112570 MtrunA17_Chr8g0354821 32.220 509 240 17 42 488 191 656 9.80e-57 201
Msa0112570 MtrunA17_Chr7g0255181 31.772 491 246 19 65 505 245 696 1.26e-56 201
Msa0112570 MtrunA17_Chr3g0080761 29.348 552 267 19 38 510 153 660 1.13e-53 192
Msa0112570 MtrunA17_Chr8g0368911 30.705 482 248 15 63 488 297 748 1.16e-53 193
Msa0112570 MtrunA17_Chr8g0368901 31.743 482 241 16 64 488 305 755 4.86e-53 191
Msa0112570 MtrunA17_Chr6g0476121 30.392 510 237 16 75 505 164 634 8.37e-53 189
Msa0112570 MtrunA17_Chr6g0476111 30.196 510 238 16 75 505 215 685 1.88e-52 189
Msa0112570 MtrunA17_Chr7g0232981 29.749 558 244 18 4 504 40 506 3.80e-52 185
Msa0112570 MtrunA17_Chr7g0252211 29.608 510 240 16 76 505 278 748 2.38e-51 187
Msa0112570 MtrunA17_Chr8g0369301 30.916 524 262 21 25 488 252 735 3.15e-51 186
Msa0112570 MtrunA17_Chr7g0232811 29.872 549 237 18 25 505 62 530 4.46e-50 180
Msa0112570 MtrunA17_Chr1g0163051 31.315 479 242 16 65 488 198 644 2.03e-49 180
Msa0112570 MtrunA17_Chr6g0467531 31.263 467 233 17 76 488 130 562 1.23e-44 165
Msa0112570 MtrunA17_Chr1g0163041 29.735 491 236 17 65 488 115 563 4.84e-44 164
Msa0112570 MtrunA17_Chr6g0467421 31.049 467 234 17 76 488 130 562 5.00e-44 164
Msa0112570 MtrunA17_Chr6g0467381 31.263 467 233 17 76 488 130 562 7.22e-44 163
Msa0112570 MtrunA17_Chr6g0467401 31.049 467 234 17 76 488 130 562 2.58e-42 159
Msa0112570 MtrunA17_Chr7g0232841 29.009 555 220 22 25 505 65 519 1.58e-41 156
Msa0112570 MtrunA17_Chr6g0467411 27.992 518 254 19 25 488 28 480 9.03e-39 147
Msa0112570 MtrunA17_Chr3g0094881 28.051 467 246 14 76 488 191 621 3.87e-35 139
Msa0112570 MtrunA17_Chr5g0403241 30.592 304 147 8 271 511 1204 1506 7.22e-28 118
Msa0112570 MtrunA17_Chr8g0373821 29.927 274 135 9 271 489 245 516 3.65e-26 112
Msa0112570 MtrunA17_Chr4g0075321 36.782 174 88 5 337 489 869 1041 4.34e-25 109
Msa0112570 MtrunA17_Chr2g0306961 31.863 204 100 6 322 488 260 461 7.16e-23 101
Msa0112570 MtrunA17_Chr2g0306961 44.037 109 44 3 76 175 79 179 4.42e-12 68.2
Msa0112570 MtrunA17_Chr1g0183321 27.740 292 131 13 276 488 428 718 8.85e-21 96.3
Msa0112570 MtrunA17_Chr1g0190311 27.723 303 134 12 268 488 317 616 3.65e-20 94.0
Msa0112570 MtrunA17_Chr7g0262001 27.852 298 139 12 267 488 377 674 2.60e-19 91.7
Msa0112570 MtrunA17_Chr1g0201311 40.268 149 66 7 356 489 61 201 8.63e-19 89.4
Msa0112570 MtrunA17_Chr1g0201621 39.597 149 67 7 356 489 61 201 4.93e-18 87.0
Msa0112570 MtrunA17_Chr7g0232751 43.373 83 42 1 428 505 28 110 4.14e-17 77.4
Msa0112570 MtrunA17_Chr2g0305371 32.057 209 103 9 292 488 268 449 2.20e-15 78.6
Msa0112570 MtrunA17_Chr3g0125251 32.911 158 87 6 360 505 1306 1456 9.68e-14 74.7
Msa0112570 MtrunA17_Chr7g0232861 34.857 175 89 7 25 182 61 227 1.06e-13 70.9
Msa0112570 MtrunA17_Chr1g0149611 36.719 128 62 4 373 489 365 484 1.16e-13 73.2
Msa0112570 MtrunA17_Chr7g0232761 32.632 190 101 8 33 207 88 265 8.38e-13 68.9
Msa0112570 MtrunA17_Chr7g0232871 47.368 57 28 1 370 426 22 76 2.76e-12 63.5
Msa0112570 MtrunA17_Chr1g0149581 32.667 150 79 7 373 510 1523 1662 1.38e-11 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0112570 AT2G35160.5 38.320 488 233 18 57 511 305 757 8.89e-87 283
Msa0112570 AT2G35160.2 38.934 488 230 18 57 511 341 793 1.65e-86 283
Msa0112570 AT2G35160.3 38.934 488 230 18 57 511 341 793 1.65e-86 283
Msa0112570 AT2G35160.1 38.934 488 230 18 57 511 341 793 1.65e-86 283
Msa0112570 AT2G35160.4 38.934 488 230 18 57 511 341 793 1.65e-86 283
Msa0112570 AT2G22740.2 34.011 541 224 17 41 505 298 781 1.16e-73 249
Msa0112570 AT2G22740.1 34.011 541 224 17 41 505 298 781 1.16e-73 249
Msa0112570 AT1G73100.1 34.110 472 219 17 73 488 202 637 4.68e-60 210
Msa0112570 AT5G04940.2 33.198 494 232 19 73 505 205 661 1.38e-56 200
Msa0112570 AT5G04940.1 33.198 494 232 19 73 505 205 661 1.38e-56 200
Msa0112570 AT2G33290.1 29.939 491 263 17 64 504 191 650 4.59e-47 174
Msa0112570 AT2G33290.3 29.939 491 263 17 64 504 191 650 4.59e-47 174
Msa0112570 AT2G33290.4 29.939 491 263 17 64 504 191 650 4.59e-47 174
Msa0112570 AT2G33290.2 29.939 491 263 17 64 504 191 650 4.59e-47 174
Msa0112570 AT4G13460.1 30.208 480 234 18 71 488 196 636 1.00e-46 173
Msa0112570 AT4G13460.2 30.208 480 234 18 71 488 196 636 1.00e-46 173
Msa0112570 AT5G13960.2 30.315 508 248 18 76 510 12 486 1.03e-45 167
Msa0112570 AT5G13960.1 30.315 508 248 18 76 510 146 620 4.11e-45 168
Msa0112570 AT1G17770.1 28.824 510 250 20 73 510 221 689 9.14e-45 168
Msa0112570 AT2G24740.1 30.290 482 248 17 73 505 304 746 3.56e-42 161
Msa0112570 AT2G23740.3 32.646 291 134 11 252 488 1073 1355 4.34e-29 122
Msa0112570 AT2G23740.6 32.646 291 134 11 252 488 1073 1355 4.34e-29 122
Msa0112570 AT2G23740.2 32.646 291 134 11 252 488 1073 1355 4.34e-29 122
Msa0112570 AT2G23740.1 32.646 291 134 11 252 488 1066 1348 4.78e-29 122
Msa0112570 AT5G47150.1 45.033 151 64 5 73 213 170 311 1.11e-23 102
Msa0112570 AT5G47160.1 44.737 152 69 3 73 217 256 399 1.67e-23 103
Msa0112570 AT5G47160.3 44.737 152 69 3 73 217 256 399 1.67e-23 103
Msa0112570 AT5G47160.2 44.737 152 69 3 73 217 256 399 1.67e-23 103
Msa0112570 AT3G04380.3 24.688 401 189 18 183 488 36 418 6.20e-19 90.1
Msa0112570 AT3G04380.1 24.688 401 189 18 183 488 52 434 6.79e-19 90.1
Msa0112570 AT3G04380.4 24.688 401 189 18 183 488 52 434 7.60e-19 89.7
Msa0112570 AT3G04380.2 25.806 310 148 12 259 488 100 407 8.14e-18 86.7
Msa0112570 AT3G04380.5 25.806 310 148 12 259 488 84 391 9.49e-18 86.3
Msa0112570 AT3G04380.6 25.806 310 148 12 259 488 100 407 1.15e-17 85.9
Msa0112570 AT2G23740.4 30.400 250 112 11 252 447 1073 1314 2.58e-17 86.3
Msa0112570 AT2G23740.5 30.400 250 112 11 252 447 1073 1314 2.58e-17 86.3
Msa0112570 AT3G03750.2 35.484 155 77 4 356 488 169 322 1.17e-16 82.0
Msa0112570 AT5G43990.16 33.962 159 85 6 349 488 501 658 2.72e-16 82.4
Msa0112570 AT5G43990.5 33.962 159 85 6 349 488 501 658 2.72e-16 82.4
Msa0112570 AT5G43990.17 33.962 159 85 6 349 488 501 658 2.72e-16 82.4
Msa0112570 AT5G43990.14 33.962 159 85 6 349 488 501 658 2.72e-16 82.4
Msa0112570 AT5G43990.3 33.962 159 85 6 349 488 521 678 2.96e-16 82.4
Msa0112570 AT5G43990.11 33.962 159 85 6 349 488 521 678 2.96e-16 82.4
Msa0112570 AT5G43990.1 33.962 159 85 6 349 488 521 678 2.96e-16 82.4
Msa0112570 AT5G43990.4 33.962 159 85 6 349 488 521 678 2.96e-16 82.4
Msa0112570 AT5G43990.15 33.962 159 85 6 349 488 524 681 3.05e-16 82.4
Msa0112570 AT5G43990.10 33.962 159 85 6 349 488 524 681 3.05e-16 82.4
Msa0112570 AT5G43990.7 33.962 159 85 6 349 488 432 589 3.24e-16 82.0
Msa0112570 AT5G43990.9 33.962 159 85 6 349 488 544 701 3.28e-16 82.4
Msa0112570 AT5G43990.2 33.962 159 85 6 349 488 544 701 3.28e-16 82.4
Msa0112570 AT5G43990.8 33.962 159 85 6 349 488 452 609 3.84e-16 82.0
Msa0112570 AT5G43990.12 33.962 159 85 6 349 488 452 609 3.84e-16 82.0
Msa0112570 AT5G43990.6 33.962 159 85 6 349 488 452 609 3.84e-16 82.0
Msa0112570 AT5G43990.13 33.962 159 85 6 349 488 452 609 3.84e-16 82.0
Msa0112570 AT1G04050.2 33.333 159 87 5 349 488 537 695 7.06e-16 81.3
Msa0112570 AT1G04050.1 33.333 159 87 5 349 488 537 695 7.06e-16 81.3
Msa0112570 AT1G04050.3 33.333 159 87 5 349 488 433 591 8.04e-16 80.9
Msa0112570 AT4G30860.1 36.429 140 77 4 357 488 307 442 4.28e-14 75.1
Msa0112570 AT1G77300.2 30.323 155 97 4 357 505 1009 1158 1.86e-13 73.9
Msa0112570 AT1G77300.1 30.323 155 97 4 357 505 1009 1158 1.86e-13 73.9
Msa0112570 AT3G59960.3 32.867 143 74 4 372 505 112 241 5.17e-12 67.0
Msa0112570 AT3G59960.2 32.867 143 74 4 372 505 112 241 5.17e-12 67.0
Msa0112570 AT3G59960.4 32.867 143 74 4 372 505 112 241 5.17e-12 67.0
Msa0112570 AT3G59960.1 32.867 143 74 4 372 505 112 241 1.08e-11 66.6
Msa0112570 AT3G03750.1 34.228 149 71 5 356 488 169 306 1.61e-11 66.2

Find 129 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGATTTAAATGTCTAATT+AGG 0.061614 1_3:-27549805 Msa0112570:three_prime_UTR
CTCATGTGGTATCGATTATT+TGG 0.102559 1_3:-27552645 Msa0112570:CDS
AATTATGTAATAGTATAATT+AGG 0.173935 1_3:-27549674 Msa0112570:three_prime_UTR
TACATGCCCTATTCTCTTTC+CGG 0.210201 1_3:+27552739 None:intergenic
TGATGACTATATAATTAATA+TGG 0.212544 1_3:-27551613 Msa0112570:CDS
ATGCAAAGTTCAAAATCTTT+TGG 0.219974 1_3:-27552995 Msa0112570:CDS
TGGGCGCTTACTGAAGAATT+TGG 0.221187 1_3:-27549862 Msa0112570:CDS
GCGACACCGTCCCTTATATT+AGG 0.242119 1_3:+27552518 None:intergenic
CTTCAGAATTGTGTCTCTTC+TGG 0.280628 1_3:+27552216 None:intergenic
TCCTGTTAGGGTTATATTGC+AGG 0.288637 1_3:-27552432 Msa0112570:CDS
CTGATTTAAATGTCTAATTA+GGG 0.295750 1_3:-27549804 Msa0112570:three_prime_UTR
TATATAAAGGATGATTTCAT+AGG 0.299705 1_3:+27551846 None:intergenic
GTTCGGCCCTATATTGAAAT+TGG 0.307938 1_3:+27552696 None:intergenic
TACCTTTAGTATAATCCTAT+TGG 0.309534 1_3:-27549635 Msa0112570:three_prime_UTR
TAATTAGTACCCCTCGAAAA+AGG 0.317377 1_3:-27549702 Msa0112570:three_prime_UTR
GTATCGATTATTTGGGTAAA+GGG 0.327727 1_3:-27552637 Msa0112570:CDS
TGTTCGAATCAATGATATTT+CGG 0.328396 1_3:-27552093 Msa0112570:CDS
ACTAGGTAACCGAAGGGTTT+AGG 0.329178 1_3:+27552014 None:intergenic
TGTTTCCCCTCGAGATGTTA+TGG 0.333335 1_3:-27552246 Msa0112570:CDS
CGCGCCTCATGATCAGAAAT+TGG 0.347176 1_3:-27552498 Msa0112570:CDS
ACTCGAGTAAGAGGACAATT+TGG 0.352436 1_3:-27552320 Msa0112570:CDS
TGGTGACCAATTTCAATATA+GGG 0.354159 1_3:-27552702 Msa0112570:CDS
GATCGAATATGTTTACCAAT+AGG 0.355001 1_3:+27549620 None:intergenic
CGCGCGGAAATCTTGCTTTG+TGG 0.366478 1_3:-27552475 Msa0112570:CDS
ATGGTGGTAACCCTAATATA+AGG 0.370183 1_3:-27552529 Msa0112570:CDS
ACCTGCAATATAACCCTAAC+AGG 0.380684 1_3:+27552431 None:intergenic
GGGTAAAGGGGAGGGTTCTT+TGG 0.380696 1_3:-27552624 Msa0112570:CDS
ATTATGTAATAGTATAATTA+GGG 0.380888 1_3:-27549673 Msa0112570:three_prime_UTR
TGAATGCGGGGAGTCCTGTT+AGG 0.386175 1_3:-27552445 Msa0112570:CDS
CGACACCGTCCCTTATATTA+GGG 0.395566 1_3:+27552519 None:intergenic
TCTTATTCATACATATATGA+TGG 0.403540 1_3:-27552365 Msa0112570:CDS
TTGGTGACCAATTTCAATAT+AGG 0.405625 1_3:-27552703 Msa0112570:CDS
GTTTCCCCTCGAGATGTTAT+GGG 0.409974 1_3:-27552245 Msa0112570:CDS
GCGAATGTTATGAAGTCTTC+TGG 0.411419 1_3:-27552572 Msa0112570:intron
AGGGCCACACTCATATATAA+AGG 0.421860 1_3:+27551833 None:intergenic
TACCAATAGGATTATACTAA+AGG 0.429034 1_3:+27549633 None:intergenic
TCATGTGGTATCGATTATTT+GGG 0.431263 1_3:-27552644 Msa0112570:CDS
ACATCTTTCACAACTAGGTT+AGG 0.431941 1_3:+27551421 None:intergenic
ATAGGTGTCACCACACGAAT+AGG 0.433162 1_3:+27552053 None:intergenic
CAAAATGTTGTTTGCTACAA+AGG 0.440548 1_3:-27551365 Msa0112570:CDS
GAGCTCACACACAAAGCTTC+CGG 0.447492 1_3:+27551689 None:intergenic
GAAAACACCGGAAAGAGAAT+AGG 0.449177 1_3:-27552746 Msa0112570:CDS
TTTGTTGATTAACACGGGCT+GGG 0.451402 1_3:-27549769 Msa0112570:three_prime_UTR
AGGGCCGAACTTATTGTAAT+CGG 0.452930 1_3:-27552683 Msa0112570:CDS
ATTATGTACACCCTAATTGC+TGG 0.458743 1_3:+27549828 None:intergenic
AAGATGAATCGTTCGATGCT+AGG 0.460514 1_3:-27551975 Msa0112570:CDS
AAAACACCGGAAAGAGAATA+GGG 0.465297 1_3:-27552745 Msa0112570:CDS
GATTATTTGGGTAAAGGGGA+GGG 0.467741 1_3:-27552632 Msa0112570:CDS
ATTTGTTGATTAACACGGGC+TGG 0.471045 1_3:-27549770 Msa0112570:three_prime_UTR
AAGAGAATAGGGCATGTAGA+TGG 0.472138 1_3:-27552734 Msa0112570:CDS
AAACATATTCGATCAATGCC+TGG 0.474494 1_3:-27549611 Msa0112570:three_prime_UTR
GGTATCGATTATTTGGGTAA+AGG 0.478346 1_3:-27552638 Msa0112570:CDS
GACAATGTGACTCGAGTAAG+AGG 0.478502 1_3:-27552329 Msa0112570:CDS
TTATGAAGGCGAAACGTCAA+TGG 0.484134 1_3:-27552769 Msa0112570:CDS
GATGACTATATAATTAATAT+GGG 0.486512 1_3:-27551612 Msa0112570:intron
TACCACATGAGTGTTGTTGA+TGG 0.494297 1_3:+27552657 None:intergenic
TAAGAAAATTCAGGACCAAC+CGG 0.497017 1_3:-27552273 Msa0112570:intron
CTTATTCATACATATATGAT+GGG 0.498680 1_3:-27552364 Msa0112570:CDS
ATTGATTCGAACAACATCCT+TGG 0.502629 1_3:+27552103 None:intergenic
CGATTATTTGGGTAAAGGGG+AGG 0.503266 1_3:-27552633 Msa0112570:CDS
AGCTTTGTGTGTGAGCTCAT+TGG 0.503294 1_3:-27551684 Msa0112570:CDS
TTGTTGATTAACACGGGCTG+GGG 0.505839 1_3:-27549768 Msa0112570:three_prime_UTR
ACATAACATCTTTCACAACT+AGG 0.508844 1_3:+27551416 None:intergenic
ATGAAGAACACAAGCAAGAA+GGG 0.514647 1_3:+27551814 None:intergenic
ATCTTCAAAACAAAATTGAA+AGG 0.515011 1_3:-27551744 Msa0112570:CDS
ATATAAAGGATGATTTCATA+GGG 0.519667 1_3:+27551847 None:intergenic
CAAAACAAAATTGAAAGGGT+GGG 0.521314 1_3:-27551739 Msa0112570:CDS
AACATATTCGATCAATGCCT+GGG 0.522306 1_3:-27549610 Msa0112570:three_prime_UTR
TTGGGAGAAAGCGTGAAGAA+TGG 0.523029 1_3:-27552976 Msa0112570:CDS
GCAACTAGACATGGAAACAT+TGG 0.525331 1_3:-27551469 Msa0112570:CDS
ACACGCTCTTTCATTCCATC+CGG 0.526296 1_3:-27551708 Msa0112570:CDS
TGGTGGTAACCCTAATATAA+GGG 0.526528 1_3:-27552528 Msa0112570:CDS
TCAAAACAAAATTGAAAGGG+TGG 0.528168 1_3:-27551740 Msa0112570:CDS
AAACATTCTTTGGGAGAACA+TGG 0.531170 1_3:+27552192 None:intergenic
CATGTAGATGGAATTGAAGT+TGG 0.532327 1_3:-27552722 Msa0112570:CDS
TTGAGTAAAATTTGCTACTG+TGG 0.533623 1_3:-27551280 Msa0112570:intron
ATTTATTTGTTGATTAACAC+GGG 0.540345 1_3:-27549774 Msa0112570:three_prime_UTR
GATGAAGAACACAAGCAAGA+AGG 0.541031 1_3:+27551813 None:intergenic
GAATGCGGGGAGTCCTGTTA+GGG 0.541817 1_3:-27552444 Msa0112570:CDS
TCTTCAAAACAAAATTGAAA+GGG 0.541871 1_3:-27551743 Msa0112570:CDS
GAGACCGATTACAATAAGTT+CGG 0.543201 1_3:+27552679 None:intergenic
GTAGTGACACTGCTTTGAAA+CGG 0.543384 1_3:-27552916 Msa0112570:CDS
CGAAGCTACAAGGTTTATGA+AGG 0.543860 1_3:-27552783 Msa0112570:intron
TTGCTGCAAGGTGAATATCA+AGG 0.545402 1_3:-27551305 Msa0112570:CDS
AAAACAAAATTGAAAGGGTG+GGG 0.546151 1_3:-27551738 Msa0112570:CDS
CACTGCTTTGAAACGGAGAA+AGG 0.546980 1_3:-27552909 Msa0112570:CDS
CGCGCCAATTTCTGATCATG+AGG 0.548143 1_3:+27552494 None:intergenic
TATGAAGGCGAAACGTCAAT+GGG 0.552062 1_3:-27552768 Msa0112570:CDS
TTCATTGATGCAACTAGACA+TGG 0.553236 1_3:-27551478 Msa0112570:CDS
TCTATGAGTTACAACAATGC+TGG 0.561265 1_3:+27552598 None:intergenic
GGTGGGACATTAGCCTATGA+TGG 0.561445 1_3:-27551888 Msa0112570:CDS
GTGGAGATACCTAAACCCTT+CGG 0.568688 1_3:-27552023 Msa0112570:intron
TATTTATTTGTTGATTAACA+CGG 0.568775 1_3:-27549775 Msa0112570:three_prime_UTR
TTATCTTGTCAGAAGATGAA+AGG 0.571853 1_3:+27552939 None:intergenic
TTGGTTTCCATAACATCTCG+AGG 0.577098 1_3:+27552167 None:intergenic
CGAATCAATGATATTTCGGA+GGG 0.577253 1_3:-27552089 Msa0112570:CDS
CAGAAGAGACACAATTCTGA+AGG 0.581158 1_3:-27552215 Msa0112570:CDS
TAACTCAGCTCTCTACCTGC+GGG 0.583390 1_3:+27551337 None:intergenic
TTGTGGAATAGTATGAATGC+GGG 0.595742 1_3:-27552458 Msa0112570:CDS
CATATTGACTAGGTAACCGA+AGG 0.601280 1_3:+27552007 None:intergenic
CTAGACATGGAAACATTGGA+AGG 0.602878 1_3:-27551465 Msa0112570:CDS
TCTCGAGGGGAAACACTAGC+CGG 0.615076 1_3:+27552254 None:intergenic
TGTGTTTGCATAGCTAAGAA+TGG 0.620233 1_3:-27551909 Msa0112570:CDS
AAAGTTCCCATAACATCTCG+AGG 0.623289 1_3:+27552239 None:intergenic
TATCGATTATTTGGGTAAAG+GGG 0.626149 1_3:-27552636 Msa0112570:CDS
TCGAATCAATGATATTTCGG+AGG 0.627900 1_3:-27552090 Msa0112570:CDS
AGAAAAGTTTCCTATTCGTG+TGG 0.630138 1_3:-27552063 Msa0112570:intron
GAGGGGAAACACTAGCCGGT+TGG 0.631704 1_3:+27552258 None:intergenic
AATCGTTCGATGCTAGGTGG+TGG 0.633767 1_3:-27551969 Msa0112570:CDS
ATAACTCAGCTCTCTACCTG+CGG 0.634593 1_3:+27551336 None:intergenic
AAGTTCCCATAACATCTCGA+GGG 0.638178 1_3:+27552240 None:intergenic
TCATCCTTTATATATGAGTG+TGG 0.641509 1_3:-27551837 Msa0112570:CDS
TTCGATAAGTCATATTGACT+AGG 0.652192 1_3:+27551997 None:intergenic
GCTACAAAGGACATACCCGC+AGG 0.652394 1_3:-27551352 Msa0112570:CDS
TTTGCATAGCTAAGAATGGT+GGG 0.653656 1_3:-27551905 Msa0112570:CDS
GGTAACCCTAATATAAGGGA+CGG 0.664705 1_3:-27552524 Msa0112570:CDS
CTCCATCAACAACACTCATG+TGG 0.665071 1_3:-27552659 Msa0112570:CDS
AGTTCCCATAACATCTCGAG+GGG 0.674068 1_3:+27552241 None:intergenic
ATATTGACTAGGTAACCGAA+GGG 0.676746 1_3:+27552008 None:intergenic
TCACACACAAAGCTTCCGGA+TGG 0.681981 1_3:+27551693 None:intergenic
AAACGTCAATGGGAAAACAC+CGG 0.685237 1_3:-27552758 Msa0112570:CDS
TATAAAGGATGATTTCATAG+GGG 0.691473 1_3:+27551848 None:intergenic
ATGAATCGTTCGATGCTAGG+TGG 0.692925 1_3:-27551972 Msa0112570:CDS
TGTGGAATAGTATGAATGCG+GGG 0.700340 1_3:-27552457 Msa0112570:CDS
CATGATCAGAAATTGGCGCG+CGG 0.700635 1_3:-27552491 Msa0112570:CDS
TTTGTGGAATAGTATGAATG+CGG 0.704358 1_3:-27552459 Msa0112570:CDS
TTGCTTTATGAAGGTCATGG+TGG 0.708605 1_3:-27552545 Msa0112570:intron
GTTTGCATAGCTAAGAATGG+TGG 0.725954 1_3:-27551906 Msa0112570:CDS
TTATGATTACAATTGCTGCA+AGG 0.726923 1_3:-27551317 Msa0112570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATACATTAGTTAAAAAATA+TGG + chr1_3:27552378-27552397 None:intergenic 10.0%
!! TAGATAATTAAAGATATTAA+CGG - chr1_3:27552481-27552500 Msa0112570:CDS 10.0%
!! AATTATGTAATAGTATAATT+AGG - chr1_3:27552952-27552971 Msa0112570:CDS 10.0%
!! ATTATGTAATAGTATAATTA+GGG - chr1_3:27552953-27552972 Msa0112570:CDS 10.0%
!!! ATAAAGCATTAACAATTTTT+AGG - chr1_3:27549743-27549762 Msa0112570:three_prime_UTR 15.0%
!!! TAAAGCATTAACAATTTTTA+GGG - chr1_3:27549744-27549763 Msa0112570:three_prime_UTR 15.0%
!! TGATGACTATATAATTAATA+TGG - chr1_3:27551013-27551032 Msa0112570:intron 15.0%
!! GATGACTATATAATTAATAT+GGG - chr1_3:27551014-27551033 Msa0112570:intron 15.0%
!! TAAATGTCAATATATAATCA+AGG + chr1_3:27552710-27552729 None:intergenic 15.0%
!!! TATTTATTTGTTGATTAACA+CGG - chr1_3:27552851-27552870 Msa0112570:CDS 15.0%
!! TAAAATTGAAAGCAAAACTA+AGG - chr1_3:27549812-27549831 Msa0112570:three_prime_UTR 20.0%
!! AAAATTGAAAGCAAAACTAA+GGG - chr1_3:27549813-27549832 Msa0112570:three_prime_UTR 20.0%
!! TCTTATTCATACATATATGA+TGG - chr1_3:27550261-27550280 Msa0112570:intron 20.0%
!! CTTATTCATACATATATGAT+GGG - chr1_3:27550262-27550281 Msa0112570:intron 20.0%
!! ATATAAAGGATGATTTCATA+GGG + chr1_3:27550782-27550801 None:intergenic 20.0%
!! TATATAAAGGATGATTTCAT+AGG + chr1_3:27550783-27550802 None:intergenic 20.0%
!! ATCTTCAAAACAAAATTGAA+AGG - chr1_3:27550882-27550901 Msa0112570:intron 20.0%
!! TCTTCAAAACAAAATTGAAA+GGG - chr1_3:27550883-27550902 Msa0112570:intron 20.0%
!!! ATTAATATGGGTAATGTTTT+TGG - chr1_3:27551026-27551045 Msa0112570:intron 20.0%
!!! TTTTTGACTTATTTTAGCTT+AGG - chr1_3:27551445-27551464 Msa0112570:CDS 20.0%
!! CTGATTTAAATATGTTCATA+TGG + chr1_3:27551490-27551509 None:intergenic 20.0%
!! TCATGTTAAGTATAAAAACA+GGG + chr1_3:27551559-27551578 None:intergenic 20.0%
!! AAAAAAAAACATGACATCTT+AGG - chr1_3:27551597-27551616 Msa0112570:intron 20.0%
!!! TATTTTATTCTTTTTGAACC+AGG - chr1_3:27551651-27551670 Msa0112570:CDS 20.0%
!!! AATCAAACTTTTGCTTATAT+TGG - chr1_3:27552191-27552210 Msa0112570:CDS 20.0%
!! TGAATGCTATTAAAGAAAAT+AGG + chr1_3:27552635-27552654 None:intergenic 20.0%
!!! TTTCATCATTGTTATTTTTC+AGG - chr1_3:27552732-27552751 Msa0112570:CDS 20.0%
!!! TTCATCATTGTTATTTTTCA+GGG - chr1_3:27552733-27552752 Msa0112570:CDS 20.0%
!! CTGATTTAAATGTCTAATTA+GGG - chr1_3:27552822-27552841 Msa0112570:intron 20.0%
!!! ATTTATTTGTTGATTAACAC+GGG - chr1_3:27552852-27552871 Msa0112570:CDS 20.0%
!!! TAATTATAATCCTTTTTCGA+GGG + chr1_3:27552937-27552956 None:intergenic 20.0%
!!! ATAATTATAATCCTTTTTCG+AGG + chr1_3:27552938-27552957 None:intergenic 20.0%
!! ATGCAAAGTTCAAAATCTTT+TGG - chr1_3:27549631-27549650 Msa0112570:three_prime_UTR 25.0%
!! TGCAAAGTTCAAAATCTTTT+GGG - chr1_3:27549632-27549651 Msa0112570:three_prime_UTR 25.0%
!! AGTTTTGCTTTCAATTTTAC+AGG + chr1_3:27549811-27549830 None:intergenic 25.0%
! TGTTCGAATCAATGATATTT+CGG - chr1_3:27550533-27550552 Msa0112570:intron 25.0%
! TATAAAGGATGATTTCATAG+GGG + chr1_3:27550781-27550800 None:intergenic 25.0%
!!! TTGTTTTGAAGATTTCAAGT+TGG + chr1_3:27550875-27550894 None:intergenic 25.0%
! GTCATGTTAAGTATAAAAAC+AGG + chr1_3:27551560-27551579 None:intergenic 25.0%
! TATACTTAACATGACATCTT+AGG - chr1_3:27551565-27551584 Msa0112570:intron 25.0%
! CCATCTATTATACAACTATA+GGG + chr1_3:27551885-27551904 None:intergenic 25.0%
! TCCATCTATTATACAACTAT+AGG + chr1_3:27551886-27551905 None:intergenic 25.0%
! CTATAATATCAAGAATGTCA+TGG + chr1_3:27552113-27552132 None:intergenic 25.0%
!!! ATTGGACATGTATTTTGAAA+CGG + chr1_3:27552319-27552338 None:intergenic 25.0%
!!! CTGAAGAATTTGGAATTTTA+AGG - chr1_3:27552774-27552793 Msa0112570:CDS 25.0%
!! GCTGATTTAAATGTCTAATT+AGG - chr1_3:27552821-27552840 Msa0112570:intron 25.0%
!!! AATTATAATCCTTTTTCGAG+GGG + chr1_3:27552936-27552955 None:intergenic 25.0%
! TACCTTTAGTATAATCCTAT+TGG - chr1_3:27552991-27553010 Msa0112570:CDS 25.0%
! TACCAATAGGATTATACTAA+AGG + chr1_3:27552996-27553015 None:intergenic 25.0%
TTATCTTGTCAGAAGATGAA+AGG + chr1_3:27549690-27549709 None:intergenic 30.0%
! TTGCTTTCAATTTTACAGGT+TGG + chr1_3:27549807-27549826 None:intergenic 30.0%
! TTGGTGACCAATTTCAATAT+AGG - chr1_3:27549923-27549942 Msa0112570:intron 30.0%
TGGTGACCAATTTCAATATA+GGG - chr1_3:27549924-27549943 Msa0112570:intron 30.0%
!! TCATGTGGTATCGATTATTT+GGG - chr1_3:27549982-27550001 Msa0112570:intron 30.0%
!! GTATCGATTATTTGGGTAAA+GGG - chr1_3:27549989-27550008 Msa0112570:intron 30.0%
! TATCGATTATTTGGGTAAAG+GGG - chr1_3:27549990-27550009 Msa0112570:intron 30.0%
TTTGTGGAATAGTATGAATG+CGG - chr1_3:27550167-27550186 Msa0112570:intron 30.0%
! TTCTGAAAAATCTGAAGTGA+TGG - chr1_3:27550218-27550237 Msa0112570:intron 30.0%
!!! ATATGATGGGTTGTATTTTG+TGG - chr1_3:27550275-27550294 Msa0112570:intron 30.0%
GTTTGTGCTTAAGAAAATTC+AGG - chr1_3:27550344-27550363 Msa0112570:intron 30.0%
!!! TTGTCTTTTTCGGAAAGTTT+TGG + chr1_3:27550481-27550500 None:intergenic 30.0%
! CTCTAGACATTTGTCTTTTT+CGG + chr1_3:27550491-27550510 None:intergenic 30.0%
! TTCGATAAGTCATATTGACT+AGG + chr1_3:27550632-27550651 None:intergenic 30.0%
! AAGTTTTCTGTTACCATCAT+AGG + chr1_3:27550754-27550773 None:intergenic 30.0%
TCATCCTTTATATATGAGTG+TGG - chr1_3:27550789-27550808 Msa0112570:intron 30.0%
TCAAAACAAAATTGAAAGGG+TGG - chr1_3:27550886-27550905 Msa0112570:intron 30.0%
CAAAACAAAATTGAAAGGGT+GGG - chr1_3:27550887-27550906 Msa0112570:intron 30.0%
AAAACAAAATTGAAAGGGTG+GGG - chr1_3:27550888-27550907 Msa0112570:intron 30.0%
!!! GTTGATTTGATCCAACTTTT+TGG + chr1_3:27550989-27551008 None:intergenic 30.0%
ACATAACATCTTTCACAACT+AGG + chr1_3:27551213-27551232 None:intergenic 30.0%
! TGTAGCAAACAACATTTTGT+GGG + chr1_3:27551260-27551279 None:intergenic 30.0%
! TTGTAGCAAACAACATTTTG+TGG + chr1_3:27551261-27551280 None:intergenic 30.0%
! CAAAATGTTGTTTGCTACAA+AGG - chr1_3:27551261-27551280 Msa0112570:intron 30.0%
TTATGATTACAATTGCTGCA+AGG - chr1_3:27551309-27551328 Msa0112570:CDS 30.0%
!! TTGAGTAAAATTTGCTACTG+TGG - chr1_3:27551346-27551365 Msa0112570:CDS 30.0%
ATGGACAAATCTACATTTGA+AGG - chr1_3:27551383-27551402 Msa0112570:CDS 30.0%
TGACATCTTAGGATAAATGT+TGG - chr1_3:27551608-27551627 Msa0112570:intron 30.0%
! AGAGTATTATAGTGGGATAA+TGG - chr1_3:27551811-27551830 Msa0112570:CDS 30.0%
! GAGTATTATAGTGGGATAAT+GGG - chr1_3:27551812-27551831 Msa0112570:CDS 30.0%
CCCTATAGTTGTATAATAGA+TGG - chr1_3:27551882-27551901 Msa0112570:CDS 30.0%
GTAGTCTTTATTATAGTGAC+CGG + chr1_3:27552144-27552163 None:intergenic 30.0%
CTATAAATATATACACCCTC+CGG + chr1_3:27552220-27552239 None:intergenic 30.0%
ATAGTGCACATTGCAAAAAT+TGG + chr1_3:27552337-27552356 None:intergenic 30.0%
TAACGGTCAAAATTGTGTAT+TGG - chr1_3:27552498-27552517 Msa0112570:CDS 30.0%
!! CTTGGACATGTATTTTGAAA+CGG - chr1_3:27552539-27552558 Msa0112570:intron 30.0%
!! AATTTTAAGGACCAGCAATT+AGG - chr1_3:27552787-27552806 Msa0112570:intron 30.0%
!! ATTTTAAGGACCAGCAATTA+GGG - chr1_3:27552788-27552807 Msa0112570:intron 30.0%
GATCGAATATGTTTACCAAT+AGG + chr1_3:27553009-27553028 None:intergenic 30.0%
CTTCATAAACCTTGTAGCTT+CGG + chr1_3:27549845-27549864 None:intergenic 35.0%
AAAACACCGGAAAGAGAATA+GGG - chr1_3:27549881-27549900 Msa0112570:intron 35.0%
CATGTAGATGGAATTGAAGT+TGG - chr1_3:27549904-27549923 Msa0112570:intron 35.0%
GAGACCGATTACAATAAGTT+CGG + chr1_3:27549950-27549969 None:intergenic 35.0%
!! CTCATGTGGTATCGATTATT+TGG - chr1_3:27549981-27550000 Msa0112570:intron 35.0%
!! GGTATCGATTATTTGGGTAA+AGG - chr1_3:27549988-27550007 Msa0112570:intron 35.0%
TCTATGAGTTACAACAATGC+TGG + chr1_3:27550031-27550050 None:intergenic 35.0%
TCTGGTTGTTTGCTTTATGA+AGG - chr1_3:27550072-27550091 Msa0112570:intron 35.0%
TGTTTGCTTTATGAAGGTCA+TGG - chr1_3:27550078-27550097 Msa0112570:intron 35.0%
ATGGTGGTAACCCTAATATA+AGG - chr1_3:27550097-27550116 Msa0112570:intron 35.0%
TGGTGGTAACCCTAATATAA+GGG - chr1_3:27550098-27550117 Msa0112570:intron 35.0%
TTGTGGAATAGTATGAATGC+GGG - chr1_3:27550168-27550187 Msa0112570:intron 35.0%
TAAGAAAATTCAGGACCAAC+CGG - chr1_3:27550353-27550372 Msa0112570:intron 35.0%
! AAACATTCTTTGGGAGAACA+TGG + chr1_3:27550437-27550456 None:intergenic 35.0%
CTCGAGGAAAAACATTCTTT+GGG + chr1_3:27550446-27550465 None:intergenic 35.0%
TCTCGAGGAAAAACATTCTT+TGG + chr1_3:27550447-27550466 None:intergenic 35.0%
! TGTTTTTCCTCGAGATGTTA+TGG - chr1_3:27550452-27550471 Msa0112570:intron 35.0%
CGAAAAAGACAAATGTCTAG+AGG - chr1_3:27550489-27550508 Msa0112570:intron 35.0%
!! ATTGATTCGAACAACATCCT+TGG + chr1_3:27550526-27550545 None:intergenic 35.0%
TCGAATCAATGATATTTCGG+AGG - chr1_3:27550536-27550555 Msa0112570:intron 35.0%
CGAATCAATGATATTTCGGA+GGG - chr1_3:27550537-27550556 Msa0112570:intron 35.0%
AGAAAAGTTTCCTATTCGTG+TGG - chr1_3:27550563-27550582 Msa0112570:intron 35.0%
! ATATTGACTAGGTAACCGAA+GGG + chr1_3:27550621-27550640 None:intergenic 35.0%
TGTGTTTGCATAGCTAAGAA+TGG - chr1_3:27550717-27550736 Msa0112570:intron 35.0%
TTTGCATAGCTAAGAATGGT+GGG - chr1_3:27550721-27550740 Msa0112570:intron 35.0%
ATGAAGAACACAAGCAAGAA+GGG + chr1_3:27550815-27550834 None:intergenic 35.0%
!! TTCAAGTTGGAACTGAATAC+CGG + chr1_3:27550862-27550881 None:intergenic 35.0%
CATCACAACAACCAAAAAGT+TGG - chr1_3:27550975-27550994 Msa0112570:intron 35.0%
! TGATGTTGTATGCATTTTGC+AGG - chr1_3:27551109-27551128 Msa0112570:intron 35.0%
TTCATTGATGCAACTAGACA+TGG - chr1_3:27551148-27551167 Msa0112570:intron 35.0%
ACATCTTTCACAACTAGGTT+AGG + chr1_3:27551208-27551227 None:intergenic 35.0%
! GTAGCAAACAACATTTTGTG+GGG + chr1_3:27551259-27551278 None:intergenic 35.0%
!! AATTTGCTACTGTGGTTCAT+TGG - chr1_3:27551354-27551373 Msa0112570:CDS 35.0%
! CTTCTAAAAACCCAAGAAGA+GGG + chr1_3:27551696-27551715 None:intergenic 35.0%
GATGAAAATGGGTACCTATT+GGG - chr1_3:27551787-27551806 Msa0112570:intron 35.0%
ATGAAAATGGGTACCTATTG+GGG - chr1_3:27551788-27551807 Msa0112570:intron 35.0%
ACTATAATACTCTCCCCAAT+AGG + chr1_3:27551804-27551823 None:intergenic 35.0%
!! TATTGGGGAGAGTATTATAG+TGG - chr1_3:27551803-27551822 Msa0112570:CDS 35.0%
!! ATTGGGGAGAGTATTATAGT+GGG - chr1_3:27551804-27551823 Msa0112570:CDS 35.0%
CTAATAATACTCTCCCCAAT+GGG + chr1_3:27551840-27551859 None:intergenic 35.0%
ACTAATAATACTCTCCCCAA+TGG + chr1_3:27551841-27551860 None:intergenic 35.0%
!! ATTGGGGAGAGTATTATTAG+TGG - chr1_3:27551840-27551859 Msa0112570:CDS 35.0%
!! TTGGGGAGAGTATTATTAGT+GGG - chr1_3:27551841-27551860 Msa0112570:CDS 35.0%
ATTAGTGGGATGACAATTAG+TGG - chr1_3:27551855-27551874 Msa0112570:CDS 35.0%
GAGAAGAGTTTATAAAGAGG+AGG + chr1_3:27551973-27551992 None:intergenic 35.0%
CCTGAGAAGAGTTTATAAAG+AGG + chr1_3:27551976-27551995 None:intergenic 35.0%
CCTCTTTATAAACTCTTCTC+AGG - chr1_3:27551973-27551992 Msa0112570:CDS 35.0%
TGATATTATAGTACACCCTC+CGG - chr1_3:27552122-27552141 Msa0112570:CDS 35.0%
TCTTTATTATAGTGACCGGA+GGG + chr1_3:27552140-27552159 None:intergenic 35.0%
!! CTTTTGCTTATATTGGTGAC+CGG - chr1_3:27552198-27552217 Msa0112570:CDS 35.0%
!!! GACATGTATTTTGAAACGGA+GGG + chr1_3:27552543-27552562 None:intergenic 35.0%
!!! GACATGTATTTTGAAACGGA+GGG - chr1_3:27552543-27552562 Msa0112570:intron 35.0%
!!! TATTTTTCAGGGCAGTATGA+TGG - chr1_3:27552744-27552763 Msa0112570:CDS 35.0%
!!! ATTTTTCAGGGCAGTATGAT+GGG - chr1_3:27552745-27552764 Msa0112570:CDS 35.0%
ATTATGTACACCCTAATTGC+TGG + chr1_3:27552801-27552820 None:intergenic 35.0%
TAATTAGTACCCCTCGAAAA+AGG - chr1_3:27552924-27552943 Msa0112570:CDS 35.0%
AAACATATTCGATCAATGCC+TGG - chr1_3:27553015-27553034 Msa0112570:CDS 35.0%
!! GTAGTGACACTGCTTTGAAA+CGG - chr1_3:27549710-27549729 Msa0112570:three_prime_UTR 40.0%
CGAAGCTACAAGGTTTATGA+AGG - chr1_3:27549843-27549862 Msa0112570:exon 40.0%
TTATGAAGGCGAAACGTCAA+TGG - chr1_3:27549857-27549876 Msa0112570:CDS 40.0%
TATGAAGGCGAAACGTCAAT+GGG - chr1_3:27549858-27549877 Msa0112570:CDS 40.0%
AAACGTCAATGGGAAAACAC+CGG - chr1_3:27549868-27549887 Msa0112570:CDS 40.0%
GAAAACACCGGAAAGAGAAT+AGG - chr1_3:27549880-27549899 Msa0112570:intron 40.0%
TACATGCCCTATTCTCTTTC+CGG + chr1_3:27549890-27549909 None:intergenic 40.0%
AAGAGAATAGGGCATGTAGA+TGG - chr1_3:27549892-27549911 Msa0112570:intron 40.0%
GTTCGGCCCTATATTGAAAT+TGG + chr1_3:27549933-27549952 None:intergenic 40.0%
AGGGCCGAACTTATTGTAAT+CGG - chr1_3:27549943-27549962 Msa0112570:intron 40.0%
! TACCACATGAGTGTTGTTGA+TGG + chr1_3:27549972-27549991 None:intergenic 40.0%
GATTATTTGGGTAAAGGGGA+GGG - chr1_3:27549994-27550013 Msa0112570:intron 40.0%
GCGAATGTTATGAAGTCTTC+TGG - chr1_3:27550054-27550073 Msa0112570:intron 40.0%
TTGCTTTATGAAGGTCATGG+TGG - chr1_3:27550081-27550100 Msa0112570:intron 40.0%
GGTAACCCTAATATAAGGGA+CGG - chr1_3:27550102-27550121 Msa0112570:intron 40.0%
TGTGGAATAGTATGAATGCG+GGG - chr1_3:27550169-27550188 Msa0112570:intron 40.0%
TCCTGTTAGGGTTATATTGC+AGG - chr1_3:27550194-27550213 Msa0112570:intron 40.0%
ACCTGCAATATAACCCTAAC+AGG + chr1_3:27550198-27550217 None:intergenic 40.0%
ACTCGAGTAAGAGGACAATT+TGG - chr1_3:27550306-27550325 Msa0112570:intron 40.0%
AAGTTCCCATAACATCTCGA+GGG + chr1_3:27550389-27550408 None:intergenic 40.0%
AAAGTTCCCATAACATCTCG+AGG + chr1_3:27550390-27550409 None:intergenic 40.0%
CTTCAGAATTGTGTCTCTTC+TGG + chr1_3:27550413-27550432 None:intergenic 40.0%
CAGAAGAGACACAATTCTGA+AGG - chr1_3:27550411-27550430 Msa0112570:intron 40.0%
TTGGTTTCCATAACATCTCG+AGG + chr1_3:27550462-27550481 None:intergenic 40.0%
!! TAGAGGTTTTGATCTCTCCA+AGG - chr1_3:27550506-27550525 Msa0112570:intron 40.0%
GGTATCTCCACATAGCTTAT+AGG + chr1_3:27550594-27550613 None:intergenic 40.0%
! CATATTGACTAGGTAACCGA+AGG + chr1_3:27550622-27550641 None:intergenic 40.0%
AAGATGAATCGTTCGATGCT+AGG - chr1_3:27550651-27550670 Msa0112570:intron 40.0%
GTTTGCATAGCTAAGAATGG+TGG - chr1_3:27550720-27550739 Msa0112570:intron 40.0%
AGGGCCACACTCATATATAA+AGG + chr1_3:27550796-27550815 None:intergenic 40.0%
GATGAAGAACACAAGCAAGA+AGG + chr1_3:27550816-27550835 None:intergenic 40.0%
GCAACTAGACATGGAAACAT+TGG - chr1_3:27551157-27551176 Msa0112570:intron 40.0%
CTAGACATGGAAACATTGGA+AGG - chr1_3:27551161-27551180 Msa0112570:intron 40.0%
TTGCTGCAAGGTGAATATCA+AGG - chr1_3:27551321-27551340 Msa0112570:CDS 40.0%
TGTGGTTCATTGGAATGCAA+TGG - chr1_3:27551364-27551383 Msa0112570:CDS 40.0%
AAAACAGGGTTGAGTCTCAT+TGG + chr1_3:27551545-27551564 None:intergenic 40.0%
AAAAACCCAAGAAGAGGGTT+GGG + chr1_3:27551691-27551710 None:intergenic 40.0%
TAAAAACCCAAGAAGAGGGT+TGG + chr1_3:27551692-27551711 None:intergenic 40.0%
! GCTTCTAAAAACCCAAGAAG+AGG + chr1_3:27551697-27551716 None:intergenic 40.0%
! TGTTGTGATTTCGAGTCAAG+CGG - chr1_3:27551735-27551754 Msa0112570:CDS 40.0%
GGATGAAAATGGGTACCTAT+TGG - chr1_3:27551786-27551805 Msa0112570:intron 40.0%
AGTGGGATTTAGTCTCACAT+CGG + chr1_3:27552011-27552030 None:intergenic 40.0%
GTCTTTATTATAGTGACCGG+AGG + chr1_3:27552141-27552160 None:intergenic 40.0%
!!! GGACATGTATTTTGAAACGG+AGG + chr1_3:27552542-27552561 None:intergenic 40.0%
! GTGTATTGGCATATGTGAAG+TGG - chr1_3:27552512-27552531 Msa0112570:CDS 40.0%
CATATGTGAAGTGGTTGACT+TGG - chr1_3:27552521-27552540 Msa0112570:CDS 40.0%
!!! GGACATGTATTTTGAAACGG+AGG - chr1_3:27552542-27552561 Msa0112570:intron 40.0%
! ATTTGTTGATTAACACGGGC+TGG - chr1_3:27552856-27552875 Msa0112570:CDS 40.0%
! TTTGTTGATTAACACGGGCT+GGG - chr1_3:27552857-27552876 Msa0112570:CDS 40.0%
! TTGGGAGAAAGCGTGAAGAA+TGG - chr1_3:27549650-27549669 Msa0112570:three_prime_UTR 45.0%
!! CACTGCTTTGAAACGGAGAA+AGG - chr1_3:27549717-27549736 Msa0112570:three_prime_UTR 45.0%
CTCCATCAACAACACTCATG+TGG - chr1_3:27549967-27549986 Msa0112570:intron 45.0%
CGATTATTTGGGTAAAGGGG+AGG - chr1_3:27549993-27550012 Msa0112570:intron 45.0%
CGACACCGTCCCTTATATTA+GGG + chr1_3:27550110-27550129 None:intergenic 45.0%
GACAATGTGACTCGAGTAAG+AGG - chr1_3:27550297-27550316 Msa0112570:intron 45.0%
TGTTTCCCCTCGAGATGTTA+TGG - chr1_3:27550380-27550399 Msa0112570:intron 45.0%
GTTTCCCCTCGAGATGTTAT+GGG - chr1_3:27550381-27550400 Msa0112570:intron 45.0%
AGTTCCCATAACATCTCGAG+GGG + chr1_3:27550388-27550407 None:intergenic 45.0%
! ATAGGTGTCACCACACGAAT+AGG + chr1_3:27550576-27550595 None:intergenic 45.0%
GGTGACACCTATAAGCTATG+TGG - chr1_3:27550584-27550603 Msa0112570:intron 45.0%
GTGGAGATACCTAAACCCTT+CGG - chr1_3:27550603-27550622 Msa0112570:intron 45.0%
ACTAGGTAACCGAAGGGTTT+AGG + chr1_3:27550615-27550634 None:intergenic 45.0%
ATGAATCGTTCGATGCTAGG+TGG - chr1_3:27550654-27550673 Msa0112570:intron 45.0%
ATCAACCGAGTGAGTCAATC+CGG - chr1_3:27550840-27550859 Msa0112570:intron 45.0%
! GAATACCGGATTGACTCACT+CGG + chr1_3:27550848-27550867 None:intergenic 45.0%
ACACGCTCTTTCATTCCATC+CGG - chr1_3:27550918-27550937 Msa0112570:intron 45.0%
! AGCTTTGTGTGTGAGCTCAT+TGG - chr1_3:27550942-27550961 Msa0112570:intron 45.0%
ATAACTCAGCTCTCTACCTG+CGG + chr1_3:27551293-27551312 None:intergenic 45.0%
AAAACCCAAGAAGAGGGTTG+GGG + chr1_3:27551690-27551709 None:intergenic 45.0%
! CCATTTTCATCCCACTCTTG+CGG + chr1_3:27551778-27551797 None:intergenic 45.0%
CGCAAGAGTGGGATGAAAAT+GGG - chr1_3:27551776-27551795 Msa0112570:intron 45.0%
!! TGGGATAATGGGTACCCATT+GGG - chr1_3:27551823-27551842 Msa0112570:CDS 45.0%
GACTAAATCCCACTGAGTGT+TGG - chr1_3:27552017-27552036 Msa0112570:CDS 45.0%
TCATGGTATCAGAGCCTATC+GGG + chr1_3:27552096-27552115 None:intergenic 45.0%
GTCATGGTATCAGAGCCTAT+CGG + chr1_3:27552097-27552116 None:intergenic 45.0%
! TTGCTTATATTGGTGACCGG+AGG - chr1_3:27552201-27552220 Msa0112570:CDS 45.0%
! TGCTTATATTGGTGACCGGA+GGG - chr1_3:27552202-27552221 Msa0112570:CDS 45.0%
TGGGCGCTTACTGAAGAATT+TGG - chr1_3:27552764-27552783 Msa0112570:CDS 45.0%
! TTGTTGATTAACACGGGCTG+GGG - chr1_3:27552858-27552877 Msa0112570:CDS 45.0%
!! CTGGGGTTTTAATGTTGGCA+GGG - chr1_3:27552875-27552894 Msa0112570:CDS 45.0%
GCGACACCGTCCCTTATATT+AGG + chr1_3:27550111-27550130 None:intergenic 50.0%
CGCGCCTCATGATCAGAAAT+TGG - chr1_3:27550128-27550147 Msa0112570:intron 50.0%
CGCGCCAATTTCTGATCATG+AGG + chr1_3:27550135-27550154 None:intergenic 50.0%
CATGATCAGAAATTGGCGCG+CGG - chr1_3:27550135-27550154 Msa0112570:intron 50.0%
AATCGTTCGATGCTAGGTGG+TGG - chr1_3:27550657-27550676 Msa0112570:intron 50.0%
! GGTGGGACATTAGCCTATGA+TGG - chr1_3:27550738-27550757 Msa0112570:intron 50.0%
TCACACACAAAGCTTCCGGA+TGG + chr1_3:27550936-27550955 None:intergenic 50.0%
GAGCTCACACACAAAGCTTC+CGG + chr1_3:27550940-27550959 None:intergenic 50.0%
TAACTCAGCTCTCTACCTGC+GGG + chr1_3:27551292-27551311 None:intergenic 50.0%
TGGGGTTTGAGTAGAAGTCC+TGG + chr1_3:27551672-27551691 None:intergenic 50.0%
TCAAACCCCAACCCTCTTCT+TGG - chr1_3:27551682-27551701 Msa0112570:CDS 50.0%
CAAACCCCAACCCTCTTCTT+GGG - chr1_3:27551683-27551702 Msa0112570:CDS 50.0%
CGGAAACATACCGCAAGAGT+GGG - chr1_3:27551765-27551784 Msa0112570:intron 50.0%
CCGCAAGAGTGGGATGAAAA+TGG - chr1_3:27551775-27551794 Msa0112570:intron 50.0%
!! GTGGGATAATGGGTACCCAT+TGG - chr1_3:27551822-27551841 Msa0112570:CDS 50.0%
!! GGGATAATGGGTACCCATTG+GGG - chr1_3:27551824-27551843 Msa0112570:CDS 50.0%
GTTAGTGGCCAACACTCAGT+GGG + chr1_3:27552028-27552047 None:intergenic 50.0%
TGTTAGTGGCCAACACTCAG+TGG + chr1_3:27552029-27552048 None:intergenic 50.0%
!!! ACGGGCTGGGGTTTTAATGT+TGG - chr1_3:27552870-27552889 Msa0112570:CDS 50.0%
!! GCTGGGGTTTTAATGTTGGC+AGG - chr1_3:27552874-27552893 Msa0112570:CDS 50.0%
! GGGCTTTAGCCGAAGCTACA+AGG - chr1_3:27549833-27549852 Msa0112570:three_prime_UTR 55.0%
GGGTAAAGGGGAGGGTTCTT+TGG - chr1_3:27550002-27550021 Msa0112570:intron 55.0%
CGCGCGGAAATCTTGCTTTG+TGG - chr1_3:27550151-27550170 Msa0112570:intron 55.0%
TGAATGCGGGGAGTCCTGTT+AGG - chr1_3:27550181-27550200 Msa0112570:intron 55.0%
GAATGCGGGGAGTCCTGTTA+GGG - chr1_3:27550182-27550201 Msa0112570:intron 55.0%
TCTCGAGGGGAAACACTAGC+CGG + chr1_3:27550375-27550394 None:intergenic 55.0%
GCTACAAAGGACATACCCGC+AGG - chr1_3:27551274-27551293 Msa0112570:intron 55.0%
TCGAGTCAAGCGGAGTCAAG+CGG - chr1_3:27551745-27551764 Msa0112570:CDS 55.0%
GCGGAAACATACCGCAAGAG+TGG - chr1_3:27551764-27551783 Msa0112570:intron 55.0%
ATCGTTGGGCTTGCATCGTC+GGG + chr1_3:27552070-27552089 None:intergenic 55.0%
CATCGTTGGGCTTGCATCGT+CGG + chr1_3:27552071-27552090 None:intergenic 55.0%
AGCCTATCGGGTCCATCGTT+GGG + chr1_3:27552084-27552103 None:intergenic 55.0%
GAGGGGAAACACTAGCCGGT+TGG + chr1_3:27550371-27550390 None:intergenic 60.0%
! AGCGTGAGGGGGTGTGTTAG+TGG + chr1_3:27552043-27552062 None:intergenic 60.0%
ATCGTCGGGCTGAAGCGTGA+GGG + chr1_3:27552056-27552075 None:intergenic 60.0%
CGACGATGCAAGCCCAACGA+TGG - chr1_3:27552069-27552088 Msa0112570:CDS 60.0%
AGCCCAACGATGGACCCGAT+AGG - chr1_3:27552079-27552098 Msa0112570:CDS 60.0%
GAGCCTATCGGGTCCATCGT+TGG + chr1_3:27552085-27552104 None:intergenic 60.0%
TCGTCGGGCTGAAGCGTGAG+GGG + chr1_3:27552055-27552074 None:intergenic 65.0%
CATCGTCGGGCTGAAGCGTG+AGG + chr1_3:27552057-27552076 None:intergenic 65.0%
CGTCGGGCTGAAGCGTGAGG+GGG + chr1_3:27552054-27552073 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 27549610 27553038 27549610 ID=Msa0112570;Name=Msa0112570
chr1_3 mRNA 27549610 27553038 27549610 ID=Msa0112570-mRNA-1;Parent=Msa0112570;Name=Msa0112570-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0.12|1|0.14|0|8|244|517
chr1_3 exon 27549610 27549894 27549610 ID=Msa0112570-mRNA-1:exon:9194;Parent=Msa0112570-mRNA-1
chr1_3 exon 27551281 27551517 27551281 ID=Msa0112570-mRNA-1:exon:9193;Parent=Msa0112570-mRNA-1
chr1_3 exon 27551613 27551778 27551613 ID=Msa0112570-mRNA-1:exon:9192;Parent=Msa0112570-mRNA-1
chr1_3 exon 27551800 27552039 27551800 ID=Msa0112570-mRNA-1:exon:9191;Parent=Msa0112570-mRNA-1
chr1_3 exon 27552064 27552282 27552064 ID=Msa0112570-mRNA-1:exon:9190;Parent=Msa0112570-mRNA-1
chr1_3 exon 27552313 27552554 27552313 ID=Msa0112570-mRNA-1:exon:9189;Parent=Msa0112570-mRNA-1
chr1_3 exon 27552573 27552793 27552573 ID=Msa0112570-mRNA-1:exon:9188;Parent=Msa0112570-mRNA-1
chr1_3 exon 27552851 27553038 27552851 ID=Msa0112570-mRNA-1:exon:9187;Parent=Msa0112570-mRNA-1
chr1_3 CDS 27552851 27553038 27552851 ID=Msa0112570-mRNA-1:cds;Parent=Msa0112570-mRNA-1
chr1_3 CDS 27552573 27552793 27552573 ID=Msa0112570-mRNA-1:cds;Parent=Msa0112570-mRNA-1
chr1_3 CDS 27552313 27552554 27552313 ID=Msa0112570-mRNA-1:cds;Parent=Msa0112570-mRNA-1
chr1_3 CDS 27552064 27552282 27552064 ID=Msa0112570-mRNA-1:cds;Parent=Msa0112570-mRNA-1
chr1_3 CDS 27551800 27552039 27551800 ID=Msa0112570-mRNA-1:cds;Parent=Msa0112570-mRNA-1
chr1_3 CDS 27551613 27551778 27551613 ID=Msa0112570-mRNA-1:cds;Parent=Msa0112570-mRNA-1
chr1_3 CDS 27551281 27551517 27551281 ID=Msa0112570-mRNA-1:cds;Parent=Msa0112570-mRNA-1
chr1_3 CDS 27549854 27549894 27549854 ID=Msa0112570-mRNA-1:cds;Parent=Msa0112570-mRNA-1
chr1_3 three_prime_UTR 27549610 27549853 27549610 ID=Msa0112570-mRNA-1:three_prime_utr;Parent=Msa0112570-mRNA-1
Gene Sequence

>Msa0112570

GTGTTTTATTTGCTGATGAAGATGCAAAGTTCAAAATCTTTTGGGAGAAAGCGTGAAGAATGGTCATATACTGAGAACCTTTCATCTTCTGACAAGATAAGTAGTGACACTGCTTTGAAACGGAGAAAGGTGAATAAAGCATTAACAATTTTTAGGGACTTATCACACAAACTCTATTGTGAATACAAGTTTATGAAGGCGAAACGTCAATGGGAAAACACCGGAAAGAGAATAGGGCATGTAGATGGAATTGAAGTTGGTGACCAATTTCAATATAGGGCCGAACTTATTGTAATCGGTCTCCATCAACAACACTCATGTGGTATCGATTATTTGGGTAAAGGGGAGGGTTCTTTGGCAACCAGCATTGTTGTAACTCATAGATATGCGAATGTTATGAAGTCTTCTGGTCATGGTGGTAACCCTAATATAAGGGACGGTGTCGCGCCTCATGATCAGAAATTGGCGCGCGGAAATCTTGCTTTGTGGAATAGTATGAATGCGGGGAGTCCTGTTAGGGTTATATTGCAGGTTTCTGAAAAATCTGAAGTGATGGAAGAGTTGAGCAACAATGTTTCTTATTCATACATATATGATGGGTTGTATTTTGTGGACAATGTGACTCGAGTAAGAGGACAATTTGGAAAACTTGACCAACCGGCTAGTGTTTCCCCTCGAGATGTTATGGGAACTTTCCAGAAGAGACACAATTCTGAAGGCCATGTTCTCCCAAAGAATGTTTTTCCTCGAGATGTTATGGAAACCAAAACTTTCCGAAAAAGACAAATGTCTAGAGGTTTTGATCTCTCCAAGGATGTTGTTCGAATCAATGATATTTCGGAGGGCAAAGAAAAGTTTCCTATTCGTGTGATACCTAAACCCTTCGGTTACCTAGTCAATATGACTTATCGAAAGATGAATCGTTCGATGCTAGGTGGTGGATGTAATTGTGCGAATGCATGTATTGATTGTCTTGAATGTGTTTGCATAGCTAAGAATGGTGGGACATTAGCCTATGATGGTAACAGAAAACTTGTTTCCCCTATGAAATCATCCTTTATATATGAGTGTGGCCCTTCTTGCTTGTGTTCTTCATCTTGCATCAACCGATTCCAACTTGAAATCTTCAAAACAAAATTGAAAGGGTGGGGTGTTCGAACACGCTCTTTCATTCCATCCGGAAGCTTTGTGTGTGAGCTCATTGGAGAAGTTATTCATCACAACAACCAAAAAGTTGGATCAAATCAACATGTTGATGACTATATAATTAATATGGGTGCTTCTAGCAAGATCTTCATTGATGCAACTAGACATGGAAACATTGGAAGGTTTATCAATCATAGTTGTTCTCCTAACCTAGTTGTGAAAGATGTTATGTATGATCACAGTGATAAGAGTTTACCCCACAAAATGTTGTTTGCTACAAAGGACATACCCGCAGGTAGAGAGCTGAGTTATGATTACAATTGCTGCAAGGTGAATATCAAGGTTTTGAGTAAAATTTGCTACTGTGGGCAGTATGATGGGCGCTTACTGAAGAATTTGGAATTTTAA
Protein sequence

>Msa0112570

VFYLLMKMQSSKSFGRKREEWSYTENLSSSDKISSDTALKRRKVNKALTIFRDLSHKLYCEYKFMKAKRQWENTGKRIGHVDGIEVGDQFQYRAELIVIGLHQQHSCGIDYLGKGEGSLATSIVVTHRYANVMKSSGHGGNPNIRDGVAPHDQKLARGNLALWNSMNAGSPVRVILQVSEKSEVMEELSNNVSYSYIYDGLYFVDNVTRVRGQFGKLDQPASVSPRDVMGTFQKRHNSEGHVLPKNVFPRDVMETKTFRKRQMSRGFDLSKDVVRINDISEGKEKFPIRVIPKPFGYLVNMTYRKMNRSMLGGGCNCANACIDCLECVCIAKNGGTLAYDGNRKLVSPMKSSFIYECGPSCLCSSSCINRFQLEIFKTKLKGWGVRTRSFIPSGSFVCELIGEVIHHNNQKVGSNQHVDDYIINMGASSKIFIDATRHGNIGRFINHSCSPNLVVKDVMYDHSDKSLPHKMLFATKDIPAGRELSYDYNCCKVNIKVLSKICYCGQYDGRLLKNLEF*