Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1078150 | KAG2660576.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 28 | 130 | 9.14e-66 | 204 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1078150 | sp|P0CG89|H4_SOYBN | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.06e-67 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1078150 | A0A453MZH1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 31 | 133 | 3.54e-66 | 204 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0606580 | Msa1078150 | 0.811818 | 5.786574e-51 | -8.615850e-47 |
| Msa0647280 | Msa1078150 | 0.820230 | 7.656020e-53 | -8.615850e-47 |
| Msa0686800 | Msa1078150 | 0.812215 | 4.743460e-51 | -8.615850e-47 |
| Msa0775060 | Msa1078150 | 0.800786 | 1.224195e-48 | -8.615850e-47 |
| Msa0223080 | Msa1078150 | 0.811404 | 7.120483e-51 | -8.615850e-47 |
| Msa0460780 | Msa1078150 | 0.824453 | 8.007100e-54 | -8.615850e-47 |
| Msa1078150 | Msa1078170 | 0.838051 | 3.618642e-57 | -8.615850e-47 |
| Msa1078150 | Msa1085260 | 0.814517 | 1.480204e-51 | -8.615850e-47 |
| Msa1078150 | Msa1251160 | 0.827097 | 1.888575e-54 | -8.615850e-47 |
| Msa1078150 | Msa1306910 | 0.806101 | 9.685571e-50 | -8.615850e-47 |
| Msa1078150 | Msa1421570 | 0.984897 | 6.489861e-162 | -8.615850e-47 |
| Msa1078150 | Msa1440880 | 0.818879 | 1.557049e-52 | -8.615850e-47 |
| Msa1078150 | Msa1446810 | 0.805329 | 1.407296e-49 | -8.615850e-47 |
| Msa1078150 | Msa1446820 | 0.807346 | 5.288044e-50 | -8.615850e-47 |
| Msa1078150 | Msa1451160 | 0.811522 | 6.712774e-51 | -8.615850e-47 |
| Msa0302160 | Msa1078150 | 0.812944 | 3.285719e-51 | -8.615850e-47 |
| Msa0339490 | Msa1078150 | 0.809351 | 1.975672e-50 | -8.615850e-47 |
| Msa0577580 | Msa1078150 | 0.800073 | 1.709932e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1078150 | MtrunA17_Chr4g0072411 | 100.000 | 103 | 0 | 0 | 1 | 103 | 59 | 161 | 5.39e-69 | 203 |
| Msa1078150 | MtrunA17_Chr5g0425951 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1078150 | MtrunA17_Chr4g0030431 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1078150 | MtrunA17_Chr3g0100121 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1078150 | MtrunA17_Chr3g0113681 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1078150 | MtrunA17_Chr2g0327641 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1078150 | MtrunA17_Chr3g0103351 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1078150 | MtrunA17_Chr8g0352791 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1078150 | MtrunA17_Chr3g0103821 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1078150 | MtrunA17_Chr3g0104031 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1078150 | MtrunA17_Chr7g0225731 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1078150 | MtrunA17_Chr3g0103801 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1078150 | MtrunA17_Chr3g0103881 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1078150 | MtrunA17_Chr7g0262791 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1078150 | AT3G53730.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1078150 | AT1G07820.2 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1078150 | AT1G07820.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1078150 | AT5G59690.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1078150 | AT3G46320.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1078150 | AT2G28740.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1078150 | AT1G07660.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1078150 | AT3G45930.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1078150 | AT5G59970.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1078150 | AT5G59970.2 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1078150 | AT1G07660.2 | 83.495 | 103 | 0 | 1 | 1 | 103 | 1 | 86 | 4.52e-51 | 155 |
Find 31 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGTGTTCTCAAGATCTTCT+TGG | 0.324000 | 7_2:+77049436 | Msa1078150:CDS |
| GGAAAGGGATTGGGAAAGGG+AGG | 0.412951 | 7_2:+77049290 | Msa1078150:CDS |
| TCGCAGGCTTTGTTATGCCT+TGG | 0.436279 | 7_2:-77049349 | None:intergenic |
| AAAATGTCTGGCCGCGGAAA+AGG | 0.446222 | 7_2:+77049266 | None:intergenic |
| TGTTTATGCTTTGAAGAGAC+AGG | 0.447929 | 7_2:+77049529 | Msa1078150:CDS |
| GGAGGAAAGGGATTGGGAAA+GGG | 0.462159 | 7_2:+77049287 | Msa1078150:CDS |
| GGAAGGACTCTCTACGGTTT+CGG | 0.462635 | 7_2:+77049551 | Msa1078150:CDS |
| AGGAGGAAAGGGATTGGGAA+AGG | 0.466050 | 7_2:+77049286 | Msa1078150:CDS |
| TGGGAAAGGGAGGAGCAAAA+AGG | 0.473619 | 7_2:+77049300 | Msa1078150:CDS |
| GGAAAAGGAGGAAAGGGATT+GGG | 0.479340 | 7_2:+77049281 | Msa1078150:CDS |
| CGGAAAAGGAGGAAAGGGAT+TGG | 0.482086 | 7_2:+77049280 | Msa1078150:CDS |
| CTGCGATTCGTCGTCTTGCA+AGG | 0.502831 | 7_2:+77049366 | Msa1078150:CDS |
| GGCCGCGGAAAAGGAGGAAA+GGG | 0.550993 | 7_2:+77049275 | Msa1078150:CDS |
| CTTGCAAGACGACGAATCGC+AGG | 0.552192 | 7_2:-77049365 | None:intergenic |
| TTACTTACACTGAGCACGCT+AGG | 0.572434 | 7_2:+77049480 | Msa1078150:CDS |
| ATGTCTGGCCGCGGAAAAGG+AGG | 0.578066 | 7_2:+77049269 | Msa1078150:CDS |
| GAGGAAGACTGTTACCGCCA+TGG | 0.588179 | 7_2:+77049502 | Msa1078150:CDS |
| TGGCCGCGGAAAAGGAGGAA+AGG | 0.594714 | 7_2:+77049274 | Msa1078150:CDS |
| AGACAGGGAAGGACTCTCTA+CGG | 0.604561 | 7_2:+77049545 | Msa1078150:CDS |
| AGGACTCTCTACGGTTTCGG+TGG | 0.607233 | 7_2:+77049554 | Msa1078150:CDS |
| CAAAGCATAAACAACATCCA+TGG | 0.612707 | 7_2:-77049519 | None:intergenic |
| CGTCGTCTTGCAAGGCGTGG+TGG | 0.615268 | 7_2:+77049374 | Msa1078150:CDS |
| GTTTATGCTTTGAAGAGACA+GGG | 0.618996 | 7_2:+77049530 | Msa1078150:CDS |
| ATGCTTTGAAGAGACAGGGA+AGG | 0.619457 | 7_2:+77049534 | Msa1078150:CDS |
| ATTCGTCGTCTTGCAAGGCG+TGG | 0.619919 | 7_2:+77049371 | Msa1078150:CDS |
| GTTCTTCGCGATAACATCCA+AGG | 0.633409 | 7_2:+77049332 | Msa1078150:CDS |
| AGCGTGCTCAGTGTAAGTAA+CGG | 0.650649 | 7_2:-77049477 | None:intergenic |
| CTTACACTGAGCACGCTAGG+AGG | 0.678435 | 7_2:+77049483 | Msa1078150:CDS |
| GGTGGTGTGAAGAGAATCAG+TGG | 0.694412 | 7_2:+77049392 | Msa1078150:CDS |
| CTCATCTATGAAGAGACTCG+CGG | 0.697662 | 7_2:+77049416 | Msa1078150:CDS |
| AGCATAAACAACATCCATGG+CGG | 0.725411 | 7_2:-77049516 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAAAGCATAAACAACATCCA+TGG | - | chr7_2:77049522-77049541 | None:intergenic | 35.0% | |
| !! | TGTTTATGCTTTGAAGAGAC+AGG | + | chr7_2:77049529-77049548 | Msa1078150:CDS | 35.0% |
| !! | GTTTATGCTTTGAAGAGACA+GGG | + | chr7_2:77049530-77049549 | Msa1078150:CDS | 35.0% |
| AGCATAAACAACATCCATGG+CGG | - | chr7_2:77049519-77049538 | None:intergenic | 40.0% | |
| GGAAAAGGAGGAAAGGGATT+GGG | + | chr7_2:77049281-77049300 | Msa1078150:CDS | 45.0% | |
| ! | AGGAGCAAAAAGGCACAGAA+AGG | + | chr7_2:77049310-77049329 | Msa1078150:CDS | 45.0% |
| GTTCTTCGCGATAACATCCA+AGG | + | chr7_2:77049332-77049351 | Msa1078150:CDS | 45.0% | |
| CTCATCTATGAAGAGACTCG+CGG | + | chr7_2:77049416-77049435 | Msa1078150:CDS | 45.0% | |
| CGGTGTTCTCAAGATCTTCT+TGG | + | chr7_2:77049436-77049455 | Msa1078150:CDS | 45.0% | |
| AGCGTGCTCAGTGTAAGTAA+CGG | - | chr7_2:77049480-77049499 | None:intergenic | 45.0% | |
| TTACTTACACTGAGCACGCT+AGG | + | chr7_2:77049480-77049499 | Msa1078150:CDS | 45.0% | |
| !! | ATGCTTTGAAGAGACAGGGA+AGG | + | chr7_2:77049534-77049553 | Msa1078150:CDS | 45.0% |
| ! | ATCCCTTTCCTCCTTTTCCG+CGG | - | chr7_2:77049280-77049299 | None:intergenic | 50.0% |
| CGGAAAAGGAGGAAAGGGAT+TGG | + | chr7_2:77049280-77049299 | Msa1078150:CDS | 50.0% | |
| AGGAGGAAAGGGATTGGGAA+AGG | + | chr7_2:77049286-77049305 | Msa1078150:CDS | 50.0% | |
| GGAGGAAAGGGATTGGGAAA+GGG | + | chr7_2:77049287-77049306 | Msa1078150:CDS | 50.0% | |
| TGGGAAAGGGAGGAGCAAAA+AGG | + | chr7_2:77049300-77049319 | Msa1078150:CDS | 50.0% | |
| !! | TCGCAGGCTTTGTTATGCCT+TGG | - | chr7_2:77049352-77049371 | None:intergenic | 50.0% |
| GGTGGTGTGAAGAGAATCAG+TGG | + | chr7_2:77049392-77049411 | Msa1078150:CDS | 50.0% | |
| AGACAGGGAAGGACTCTCTA+CGG | + | chr7_2:77049545-77049564 | Msa1078150:CDS | 50.0% | |
| GGAAGGACTCTCTACGGTTT+CGG | + | chr7_2:77049551-77049570 | Msa1078150:CDS | 50.0% | |
| GGAAAGGGATTGGGAAAGGG+AGG | + | chr7_2:77049290-77049309 | Msa1078150:CDS | 55.0% | |
| CTTGCAAGACGACGAATCGC+AGG | - | chr7_2:77049368-77049387 | None:intergenic | 55.0% | |
| CTGCGATTCGTCGTCTTGCA+AGG | + | chr7_2:77049366-77049385 | Msa1078150:CDS | 55.0% | |
| ATTCGTCGTCTTGCAAGGCG+TGG | + | chr7_2:77049371-77049390 | Msa1078150:CDS | 55.0% | |
| CTTACACTGAGCACGCTAGG+AGG | + | chr7_2:77049483-77049502 | Msa1078150:CDS | 55.0% | |
| GAGGAAGACTGTTACCGCCA+TGG | + | chr7_2:77049502-77049521 | Msa1078150:CDS | 55.0% | |
| AGGACTCTCTACGGTTTCGG+TGG | + | chr7_2:77049554-77049573 | Msa1078150:CDS | 55.0% | |
| ATGTCTGGCCGCGGAAAAGG+AGG | + | chr7_2:77049269-77049288 | Msa1078150:CDS | 60.0% | |
| TGGCCGCGGAAAAGGAGGAA+AGG | + | chr7_2:77049274-77049293 | Msa1078150:CDS | 60.0% | |
| GGCCGCGGAAAAGGAGGAAA+GGG | + | chr7_2:77049275-77049294 | Msa1078150:CDS | 60.0% | |
| CGTCGTCTTGCAAGGCGTGG+TGG | + | chr7_2:77049374-77049393 | Msa1078150:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 77049269 | 77049580 | 77049269 | ID=Msa1078150;Name=Msa1078150 |
| chr7_2 | mRNA | 77049269 | 77049580 | 77049269 | ID=Msa1078150-mRNA-1;Parent=Msa1078150;Name=Msa1078150-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|103 |
| chr7_2 | exon | 77049269 | 77049580 | 77049269 | ID=Msa1078150-mRNA-1:exon:18824;Parent=Msa1078150-mRNA-1 |
| chr7_2 | CDS | 77049269 | 77049580 | 77049269 | ID=Msa1078150-mRNA-1:cds;Parent=Msa1078150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
