Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0775060 | GAU36262.1 | 82.667 | 75 | 10 | 2 | 1 | 74 | 1 | 73 | 8.96e-32 | 115 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0775060 | A0A2Z6NI28 | 82.667 | 75 | 10 | 2 | 1 | 74 | 1 | 73 | 4.28e-32 | 115 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0025160 | Msa0775060 | 0.825250 | 5.192487e-54 | -8.615850e-47 |
| Msa0025580 | Msa0775060 | 0.806963 | 6.371368e-50 | -8.615850e-47 |
| Msa0027280 | Msa0775060 | 0.801630 | 8.222426e-49 | -8.615850e-47 |
| Msa0054690 | Msa0775060 | 0.826945 | 2.052872e-54 | -8.615850e-47 |
| Msa0661150 | Msa0775060 | 0.831005 | 2.132845e-55 | -8.615850e-47 |
| Msa0735780 | Msa0775060 | 0.805140 | 1.541506e-49 | -8.615850e-47 |
| Msa0756490 | Msa0775060 | 0.835278 | 1.841715e-56 | -8.615850e-47 |
| Msa0761670 | Msa0775060 | 0.826136 | 3.200661e-54 | -8.615850e-47 |
| Msa0775060 | Msa1042160 | 0.819250 | 1.281485e-52 | -8.615850e-47 |
| Msa0775060 | Msa1042190 | 0.822376 | 2.448825e-53 | -8.615850e-47 |
| Msa0775060 | Msa1072940 | 0.802175 | 6.356406e-49 | -8.615850e-47 |
| Msa0775060 | Msa1078150 | 0.800786 | 1.224195e-48 | -8.615850e-47 |
| Msa0775060 | Msa1116220 | 0.805342 | 1.398012e-49 | -8.615850e-47 |
| Msa0775060 | Msa1125820 | 0.801983 | 6.959063e-49 | -8.615850e-47 |
| Msa0775060 | Msa1125990 | 0.802770 | 4.790853e-49 | -8.615850e-47 |
| Msa0775060 | Msa1160920 | 0.800548 | 1.368752e-48 | -8.615850e-47 |
| Msa0775060 | Msa1167470 | 0.807493 | 4.921706e-50 | -8.615850e-47 |
| Msa0775060 | Msa1343170 | 0.803204 | 3.897684e-49 | -8.615850e-47 |
| Msa0775060 | Msa1343180 | 0.801790 | 7.624742e-49 | -8.615850e-47 |
| Msa0775060 | Msa1359690 | 0.818718 | 1.693122e-52 | -8.615850e-47 |
| Msa0775060 | Msa1374790 | 0.801578 | 8.429382e-49 | -8.615850e-47 |
| Msa0775060 | Msa1421570 | 0.814509 | 1.485787e-51 | -8.615850e-47 |
| Msa0775060 | Msa1451820 | 0.803360 | 3.618179e-49 | -8.615850e-47 |
| Msa0419540 | Msa0775060 | 0.814618 | 1.405931e-51 | -8.615850e-47 |
| Msa0424140 | Msa0775060 | 0.812482 | 4.147465e-51 | -8.615850e-47 |
| Msa0465530 | Msa0775060 | 0.800649 | 1.305072e-48 | -8.615850e-47 |
| Msa0472950 | Msa0775060 | 0.832679 | 8.239235e-56 | -8.615850e-47 |
| Msa0275820 | Msa0775060 | 0.805819 | 1.110453e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0775060 | MtrunA17_Chr5g0448181 | 90.667 | 75 | 6 | 1 | 1 | 74 | 1 | 75 | 1.55e-32 | 107 |
| Msa0775060 | MtrunA17_Chr3g0078121 | 89.333 | 75 | 7 | 1 | 1 | 74 | 1 | 75 | 9.81e-32 | 105 |
| Msa0775060 | MtrunA17_Chr3g0078141 | 74.545 | 55 | 13 | 1 | 4 | 57 | 1 | 55 | 4.12e-24 | 85.5 |
| Msa0775060 | MtrunA17_Chr5g0448161 | 74.545 | 55 | 13 | 1 | 4 | 57 | 1 | 55 | 4.12e-24 | 85.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0775060 | AT1G31335.1 | 80.488 | 41 | 8 | 0 | 1 | 41 | 1 | 41 | 2.16e-20 | 77.0 |
Find 26 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGGTTTGGTTTGGAAAAT+TGG | 0.118432 | 5_2:-51731732 | None:intergenic |
| TACACAAACCCTCAAGTTTA+TGG | 0.161657 | 5_2:+51731646 | Msa0775060:CDS |
| ATCTTCTTCTGCTTAATTCT+TGG | 0.163666 | 5_2:+51731577 | Msa0775060:CDS |
| TTCTGCTTAATTCTTGGTTT+TGG | 0.239621 | 5_2:+51731583 | Msa0775060:CDS |
| ATTAAGCAGAAGAAGATTAA+AGG | 0.258762 | 5_2:-51731571 | None:intergenic |
| AAGGTTTGGTTTGGAAAATT+GGG | 0.306514 | 5_2:-51731731 | None:intergenic |
| GCAGCACCTGGTGGTGGAAT+TGG | 0.383062 | 5_2:-51731673 | None:intergenic |
| GTTGGAAGCAAGGTTTGGTT+TGG | 0.389775 | 5_2:-51731740 | None:intergenic |
| CAAACGTTGGAAGCAAGGTT+TGG | 0.414160 | 5_2:-51731745 | None:intergenic |
| TGGGGTGGAGGAGATGATGA+AGG | 0.483357 | 5_2:-51731712 | None:intergenic |
| GGTTTGGAAAATTGGGGTGG+AGG | 0.491141 | 5_2:-51731724 | None:intergenic |
| TTGGCATACCATAAACTTGA+GGG | 0.504350 | 5_2:-51731654 | None:intergenic |
| GAACTCAAACGTTGGAAGCA+AGG | 0.528191 | 5_2:-51731750 | None:intergenic |
| TATGATTTGAACTCAAACGT+TGG | 0.529432 | 5_2:-51731758 | None:intergenic |
| AATTAAGATGGGAATGGTAG+TGG | 0.531670 | 5_2:+51731537 | None:intergenic |
| TTTGCAGCAGCACCTGGTGG+TGG | 0.537498 | 5_2:-51731679 | None:intergenic |
| TGGAGGAGATGATGAAGGGA+AGG | 0.543125 | 5_2:-51731707 | None:intergenic |
| TACTTCTTTGGAAGAGCTAG+AGG | 0.553790 | 5_2:+51731610 | Msa0775060:CDS |
| GTAGTATTTGCAGCAGCACC+TGG | 0.554278 | 5_2:-51731685 | None:intergenic |
| GGGGTGGAGGAGATGATGAA+GGG | 0.562325 | 5_2:-51731711 | None:intergenic |
| AGAGAAAATTAAGATGGGAA+TGG | 0.575884 | 5_2:+51731531 | None:intergenic |
| AGGTTTGGTTTGGAAAATTG+GGG | 0.584840 | 5_2:-51731730 | None:intergenic |
| TTTGGTTTGGAAAATTGGGG+TGG | 0.604758 | 5_2:-51731727 | None:intergenic |
| GTATTTGCAGCAGCACCTGG+TGG | 0.631151 | 5_2:-51731682 | None:intergenic |
| GGTATGCCAATTCCACCACC+AGG | 0.644332 | 5_2:+51731667 | Msa0775060:CDS |
| ATTGGCATACCATAAACTTG+AGG | 0.668164 | 5_2:-51731655 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTAAGCAGAAGAAGATTAA+AGG | - | chr5_2:51731574-51731593 | None:intergenic | 25.0% |
| ATCTTCTTCTGCTTAATTCT+TGG | + | chr5_2:51731577-51731596 | Msa0775060:CDS | 30.0% | |
| ! | TTCTGCTTAATTCTTGGTTT+TGG | + | chr5_2:51731583-51731602 | Msa0775060:CDS | 30.0% |
| !! | AAGGTTTGGTTTGGAAAATT+GGG | - | chr5_2:51731734-51731753 | None:intergenic | 30.0% |
| !!! | GGTTTTGGTTGTTACTTCTT+TGG | + | chr5_2:51731598-51731617 | Msa0775060:CDS | 35.0% |
| TACACAAACCCTCAAGTTTA+TGG | + | chr5_2:51731646-51731665 | Msa0775060:CDS | 35.0% | |
| TTGGCATACCATAAACTTGA+GGG | - | chr5_2:51731657-51731676 | None:intergenic | 35.0% | |
| ATTGGCATACCATAAACTTG+AGG | - | chr5_2:51731658-51731677 | None:intergenic | 35.0% | |
| !! | AGGTTTGGTTTGGAAAATTG+GGG | - | chr5_2:51731733-51731752 | None:intergenic | 35.0% |
| !! | CAAGGTTTGGTTTGGAAAAT+TGG | - | chr5_2:51731735-51731754 | None:intergenic | 35.0% |
| ! | TACTTCTTTGGAAGAGCTAG+AGG | + | chr5_2:51731610-51731629 | Msa0775060:CDS | 40.0% |
| !! | TTTGGTTTGGAAAATTGGGG+TGG | - | chr5_2:51731730-51731749 | None:intergenic | 40.0% |
| ! | GTTGGAAGCAAGGTTTGGTT+TGG | - | chr5_2:51731743-51731762 | None:intergenic | 45.0% |
| CAAACGTTGGAAGCAAGGTT+TGG | - | chr5_2:51731748-51731767 | None:intergenic | 45.0% | |
| GTAGTATTTGCAGCAGCACC+TGG | - | chr5_2:51731688-51731707 | None:intergenic | 50.0% | |
| TGGAGGAGATGATGAAGGGA+AGG | - | chr5_2:51731710-51731729 | None:intergenic | 50.0% | |
| GGTTTGGAAAATTGGGGTGG+AGG | - | chr5_2:51731727-51731746 | None:intergenic | 50.0% | |
| GGTATGCCAATTCCACCACC+AGG | + | chr5_2:51731667-51731686 | Msa0775060:CDS | 55.0% | |
| GTATTTGCAGCAGCACCTGG+TGG | - | chr5_2:51731685-51731704 | None:intergenic | 55.0% | |
| GGGGTGGAGGAGATGATGAA+GGG | - | chr5_2:51731714-51731733 | None:intergenic | 55.0% | |
| TGGGGTGGAGGAGATGATGA+AGG | - | chr5_2:51731715-51731734 | None:intergenic | 55.0% | |
| !! | GCAGCACCTGGTGGTGGAAT+TGG | - | chr5_2:51731676-51731695 | None:intergenic | 60.0% |
| TTTGCAGCAGCACCTGGTGG+TGG | - | chr5_2:51731682-51731701 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 51731544 | 51731768 | 51731544 | ID=Msa0775060;Name=Msa0775060 |
| chr5_2 | mRNA | 51731544 | 51731768 | 51731544 | ID=Msa0775060-mRNA-1;Parent=Msa0775060;Name=Msa0775060-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|74 |
| chr5_2 | exon | 51731544 | 51731768 | 51731544 | ID=Msa0775060-mRNA-1:exon:15399;Parent=Msa0775060-mRNA-1 |
| chr5_2 | CDS | 51731544 | 51731768 | 51731544 | ID=Msa0775060-mRNA-1:cds;Parent=Msa0775060-mRNA-1 |
| Gene Sequence |
| Protein sequence |
