Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1421570 | sp|P0CG89|H4_SOYBN | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.06e-67 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1421570 | A0A453MZH1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 31 | 133 | 3.54e-66 | 204 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0035140 | Msa1421570 | 0.812343 | 4.446892e-51 | -8.615850e-47 |
| Msa1421570 | Msa1440880 | 0.843559 | 1.300655e-58 | -8.615850e-47 |
| Msa1421570 | Msa1446810 | 0.836904 | 7.119164e-57 | -8.615850e-47 |
| Msa1421570 | Msa1446820 | 0.822982 | 1.770122e-53 | -8.615850e-47 |
| Msa1421570 | Msa1451160 | 0.825362 | 4.886478e-54 | -8.615850e-47 |
| Msa0606580 | Msa1421570 | 0.832166 | 1.103640e-55 | -8.615850e-47 |
| Msa0647280 | Msa1421570 | 0.840429 | 8.742672e-58 | -8.615850e-47 |
| Msa0686800 | Msa1421570 | 0.833192 | 6.141133e-56 | -8.615850e-47 |
| Msa0704410 | Msa1421570 | 0.800268 | 1.560923e-48 | -8.615850e-47 |
| Msa0775060 | Msa1421570 | 0.814509 | 1.485787e-51 | -8.615850e-47 |
| Msa0831120 | Msa1421570 | 0.807667 | 4.519260e-50 | -8.615850e-47 |
| Msa0223080 | Msa1421570 | 0.824739 | 6.855036e-54 | -8.615850e-47 |
| Msa1251160 | Msa1421570 | 0.843353 | 1.476467e-58 | -8.615850e-47 |
| Msa1306910 | Msa1421570 | 0.825894 | 3.654631e-54 | -8.615850e-47 |
| Msa0419540 | Msa1421570 | 0.805069 | 1.595204e-49 | -8.615850e-47 |
| Msa0460780 | Msa1421570 | 0.832539 | 8.925187e-56 | -8.615850e-47 |
| Msa1078150 | Msa1421570 | 0.984897 | 6.489861e-162 | -8.615850e-47 |
| Msa1078170 | Msa1421570 | 0.841667 | 4.136485e-58 | -8.615850e-47 |
| Msa1085260 | Msa1421570 | 0.821254 | 4.453196e-53 | -8.615850e-47 |
| Msa1094010 | Msa1421570 | 0.806284 | 8.864338e-50 | -8.615850e-47 |
| Msa1103990 | Msa1421570 | 0.807680 | 4.491431e-50 | -8.615850e-47 |
| Msa0298470 | Msa1421570 | 0.810833 | 9.467158e-51 | -8.615850e-47 |
| Msa0302160 | Msa1421570 | 0.826124 | 3.222183e-54 | -8.615850e-47 |
| Msa0339490 | Msa1421570 | 0.823298 | 1.493612e-53 | -8.615850e-47 |
| Msa0339500 | Msa1421570 | 0.817395 | 3.371091e-52 | -8.615850e-47 |
| Msa0363990 | Msa1421570 | 0.801825 | 7.498355e-49 | -8.615850e-47 |
| Msa0577580 | Msa1421570 | 0.818254 | 2.157057e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1421570 | MtrunA17_Chr4g0072411 | 100.000 | 103 | 0 | 0 | 1 | 103 | 59 | 161 | 5.39e-69 | 203 |
| Msa1421570 | MtrunA17_Chr5g0425951 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1421570 | MtrunA17_Chr4g0030431 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1421570 | MtrunA17_Chr3g0100121 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1421570 | MtrunA17_Chr3g0113681 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1421570 | MtrunA17_Chr2g0327641 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1421570 | MtrunA17_Chr3g0103351 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1421570 | MtrunA17_Chr8g0352791 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1421570 | MtrunA17_Chr3g0103821 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1421570 | MtrunA17_Chr3g0104031 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1421570 | MtrunA17_Chr7g0225731 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1421570 | MtrunA17_Chr3g0103801 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1421570 | MtrunA17_Chr3g0103881 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1421570 | MtrunA17_Chr7g0262791 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1421570 | AT3G53730.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1421570 | AT1G07820.2 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1421570 | AT1G07820.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1421570 | AT5G59690.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1421570 | AT3G46320.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1421570 | AT2G28740.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1421570 | AT1G07660.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1421570 | AT3G45930.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1421570 | AT5G59970.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1421570 | AT5G59970.2 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1421570 | AT1G07660.2 | 83.495 | 103 | 0 | 1 | 1 | 103 | 1 | 86 | 4.52e-51 | 155 |
Find 31 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGTGTTCTCAAGATCTTCT+TGG | 0.324000 | tig0025135:+72123 | Msa1421570:CDS |
| GGAAAGGGATTGGGAAAGGG+AGG | 0.412951 | tig0025135:+71977 | Msa1421570:CDS |
| TCGCAGGCTTTGTTATGCCT+TGG | 0.436279 | tig0025135:-72036 | None:intergenic |
| AAAATGTCTGGCCGCGGAAA+AGG | 0.446222 | tig0025135:+71953 | None:intergenic |
| TGTTTATGCTTTGAAGAGAC+AGG | 0.447929 | tig0025135:+72216 | Msa1421570:CDS |
| GGAGGAAAGGGATTGGGAAA+GGG | 0.462159 | tig0025135:+71974 | Msa1421570:CDS |
| GGAAGGACTCTCTACGGTTT+CGG | 0.462635 | tig0025135:+72238 | Msa1421570:CDS |
| AGGAGGAAAGGGATTGGGAA+AGG | 0.466050 | tig0025135:+71973 | Msa1421570:CDS |
| TGGGAAAGGGAGGAGCAAAA+AGG | 0.473619 | tig0025135:+71987 | Msa1421570:CDS |
| GGAAAAGGAGGAAAGGGATT+GGG | 0.479340 | tig0025135:+71968 | Msa1421570:CDS |
| CGGAAAAGGAGGAAAGGGAT+TGG | 0.482086 | tig0025135:+71967 | Msa1421570:CDS |
| CTGCGATTCGTCGTCTTGCA+AGG | 0.502831 | tig0025135:+72053 | Msa1421570:CDS |
| GGCCGCGGAAAAGGAGGAAA+GGG | 0.550993 | tig0025135:+71962 | Msa1421570:CDS |
| CTTGCAAGACGACGAATCGC+AGG | 0.552192 | tig0025135:-72052 | None:intergenic |
| TTACTTACACTGAGCACGCT+AGG | 0.572434 | tig0025135:+72167 | Msa1421570:CDS |
| ATGTCTGGCCGCGGAAAAGG+AGG | 0.578066 | tig0025135:+71956 | Msa1421570:CDS |
| GAGGAAGACTGTTACCGCCA+TGG | 0.588179 | tig0025135:+72189 | Msa1421570:CDS |
| TGGCCGCGGAAAAGGAGGAA+AGG | 0.594714 | tig0025135:+71961 | Msa1421570:CDS |
| AGACAGGGAAGGACTCTCTA+CGG | 0.604561 | tig0025135:+72232 | Msa1421570:CDS |
| AGGACTCTCTACGGTTTCGG+TGG | 0.607233 | tig0025135:+72241 | Msa1421570:CDS |
| CAAAGCATAAACAACATCCA+TGG | 0.612707 | tig0025135:-72206 | None:intergenic |
| CGTCGTCTTGCAAGGCGTGG+TGG | 0.615268 | tig0025135:+72061 | Msa1421570:CDS |
| GTTTATGCTTTGAAGAGACA+GGG | 0.618996 | tig0025135:+72217 | Msa1421570:CDS |
| ATGCTTTGAAGAGACAGGGA+AGG | 0.619457 | tig0025135:+72221 | Msa1421570:CDS |
| ATTCGTCGTCTTGCAAGGCG+TGG | 0.619919 | tig0025135:+72058 | Msa1421570:CDS |
| GTTCTTCGCGATAACATCCA+AGG | 0.633409 | tig0025135:+72019 | Msa1421570:CDS |
| AGCGTGCTCAGTGTAAGTAA+CGG | 0.650649 | tig0025135:-72164 | None:intergenic |
| CTTACACTGAGCACGCTAGG+AGG | 0.678435 | tig0025135:+72170 | Msa1421570:CDS |
| GGTGGTGTGAAGAGAATCAG+TGG | 0.694412 | tig0025135:+72079 | Msa1421570:CDS |
| CTCATCTATGAAGAGACTCG+CGG | 0.697662 | tig0025135:+72103 | Msa1421570:CDS |
| AGCATAAACAACATCCATGG+CGG | 0.725411 | tig0025135:-72203 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAAAGCATAAACAACATCCA+TGG | - | tig0025135:72209-72228 | None:intergenic | 35.0% | |
| !! | TGTTTATGCTTTGAAGAGAC+AGG | + | tig0025135:72216-72235 | Msa1421570:CDS | 35.0% |
| !! | GTTTATGCTTTGAAGAGACA+GGG | + | tig0025135:72217-72236 | Msa1421570:CDS | 35.0% |
| AGCATAAACAACATCCATGG+CGG | - | tig0025135:72206-72225 | None:intergenic | 40.0% | |
| GGAAAAGGAGGAAAGGGATT+GGG | + | tig0025135:71968-71987 | Msa1421570:CDS | 45.0% | |
| ! | AGGAGCAAAAAGGCACAGAA+AGG | + | tig0025135:71997-72016 | Msa1421570:CDS | 45.0% |
| GTTCTTCGCGATAACATCCA+AGG | + | tig0025135:72019-72038 | Msa1421570:CDS | 45.0% | |
| CTCATCTATGAAGAGACTCG+CGG | + | tig0025135:72103-72122 | Msa1421570:CDS | 45.0% | |
| CGGTGTTCTCAAGATCTTCT+TGG | + | tig0025135:72123-72142 | Msa1421570:CDS | 45.0% | |
| AGCGTGCTCAGTGTAAGTAA+CGG | - | tig0025135:72167-72186 | None:intergenic | 45.0% | |
| TTACTTACACTGAGCACGCT+AGG | + | tig0025135:72167-72186 | Msa1421570:CDS | 45.0% | |
| !! | ATGCTTTGAAGAGACAGGGA+AGG | + | tig0025135:72221-72240 | Msa1421570:CDS | 45.0% |
| ! | ATCCCTTTCCTCCTTTTCCG+CGG | - | tig0025135:71967-71986 | None:intergenic | 50.0% |
| CGGAAAAGGAGGAAAGGGAT+TGG | + | tig0025135:71967-71986 | Msa1421570:CDS | 50.0% | |
| AGGAGGAAAGGGATTGGGAA+AGG | + | tig0025135:71973-71992 | Msa1421570:CDS | 50.0% | |
| GGAGGAAAGGGATTGGGAAA+GGG | + | tig0025135:71974-71993 | Msa1421570:CDS | 50.0% | |
| TGGGAAAGGGAGGAGCAAAA+AGG | + | tig0025135:71987-72006 | Msa1421570:CDS | 50.0% | |
| !! | TCGCAGGCTTTGTTATGCCT+TGG | - | tig0025135:72039-72058 | None:intergenic | 50.0% |
| GGTGGTGTGAAGAGAATCAG+TGG | + | tig0025135:72079-72098 | Msa1421570:CDS | 50.0% | |
| AGACAGGGAAGGACTCTCTA+CGG | + | tig0025135:72232-72251 | Msa1421570:CDS | 50.0% | |
| GGAAGGACTCTCTACGGTTT+CGG | + | tig0025135:72238-72257 | Msa1421570:CDS | 50.0% | |
| GGAAAGGGATTGGGAAAGGG+AGG | + | tig0025135:71977-71996 | Msa1421570:CDS | 55.0% | |
| CTTGCAAGACGACGAATCGC+AGG | - | tig0025135:72055-72074 | None:intergenic | 55.0% | |
| CTGCGATTCGTCGTCTTGCA+AGG | + | tig0025135:72053-72072 | Msa1421570:CDS | 55.0% | |
| ATTCGTCGTCTTGCAAGGCG+TGG | + | tig0025135:72058-72077 | Msa1421570:CDS | 55.0% | |
| CTTACACTGAGCACGCTAGG+AGG | + | tig0025135:72170-72189 | Msa1421570:CDS | 55.0% | |
| GAGGAAGACTGTTACCGCCA+TGG | + | tig0025135:72189-72208 | Msa1421570:CDS | 55.0% | |
| AGGACTCTCTACGGTTTCGG+TGG | + | tig0025135:72241-72260 | Msa1421570:CDS | 55.0% | |
| ATGTCTGGCCGCGGAAAAGG+AGG | + | tig0025135:71956-71975 | Msa1421570:CDS | 60.0% | |
| TGGCCGCGGAAAAGGAGGAA+AGG | + | tig0025135:71961-71980 | Msa1421570:CDS | 60.0% | |
| GGCCGCGGAAAAGGAGGAAA+GGG | + | tig0025135:71962-71981 | Msa1421570:CDS | 60.0% | |
| CGTCGTCTTGCAAGGCGTGG+TGG | + | tig0025135:72061-72080 | Msa1421570:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0025135 | gene | 71956 | 72267 | 71956 | ID=Msa1421570;Name=Msa1421570 |
| tig0025135 | mRNA | 71956 | 72267 | 71956 | ID=Msa1421570-mRNA-1;Parent=Msa1421570;Name=Msa1421570-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|103 |
| tig0025135 | exon | 71956 | 72267 | 71956 | ID=Msa1421570-mRNA-1:exon:2156;Parent=Msa1421570-mRNA-1 |
| tig0025135 | CDS | 71956 | 72267 | 71956 | ID=Msa1421570-mRNA-1:cds;Parent=Msa1421570-mRNA-1 |
| Gene Sequence |
| Protein sequence |
