Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1085260 | KAG2660576.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 28 | 130 | 9.14e-66 | 204 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1085260 | sp|P0CG89|H4_SOYBN | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.06e-67 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1085260 | A0A453MZH1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 31 | 133 | 3.54e-66 | 204 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001940 | Msa1085260 | 0.821178 | 4.635852e-53 | -8.615850e-47 |
| Msa0003890 | Msa1085260 | 0.815438 | 9.242379e-52 | -8.615850e-47 |
| Msa0008670 | Msa1085260 | 0.802096 | 6.598286e-49 | -8.615850e-47 |
| Msa0014990 | Msa1085260 | 0.813531 | 2.442340e-51 | -8.615850e-47 |
| Msa0017510 | Msa1085260 | 0.806884 | 6.620965e-50 | -8.615850e-47 |
| Msa0017950 | Msa1085260 | 0.810336 | 1.212757e-50 | -8.615850e-47 |
| Msa0020410 | Msa1085260 | 0.800095 | 1.692310e-48 | -8.615850e-47 |
| Msa0028420 | Msa1085260 | 0.805656 | 1.201641e-49 | -8.615850e-47 |
| Msa0047030 | Msa1085260 | 0.802578 | 5.250550e-49 | -8.615850e-47 |
| Msa0054480 | Msa1085260 | 0.819737 | 9.925733e-53 | -8.615850e-47 |
| Msa0084380 | Msa1085260 | 0.801455 | 8.932904e-49 | -8.615850e-47 |
| Msa0090360 | Msa1085260 | 0.819127 | 1.367477e-52 | -8.615850e-47 |
| Msa0115400 | Msa1085260 | 0.812597 | 3.912634e-51 | -8.615850e-47 |
| Msa0115560 | Msa1085260 | 0.800069 | 1.713397e-48 | -8.615850e-47 |
| Msa0607050 | Msa1085260 | 0.813188 | 2.905026e-51 | -8.615850e-47 |
| Msa0625720 | Msa1085260 | 0.836437 | 9.362524e-57 | -8.615850e-47 |
| Msa0643080 | Msa1085260 | 0.825646 | 4.185200e-54 | -8.615850e-47 |
| Msa0652450 | Msa1085260 | 0.819327 | 1.231148e-52 | -8.615850e-47 |
| Msa0663390 | Msa1085260 | 0.830273 | 3.221988e-55 | -8.615850e-47 |
| Msa0667050 | Msa1085260 | 0.809476 | 1.856558e-50 | -8.615850e-47 |
| Msa0687020 | Msa1085260 | 0.804427 | 2.171381e-49 | -8.615850e-47 |
| Msa0690150 | Msa1085260 | 0.816371 | 5.724464e-52 | -8.615850e-47 |
| Msa0709400 | Msa1085260 | 0.801680 | 8.031946e-49 | -8.615850e-47 |
| Msa0717890 | Msa1085260 | 0.800653 | 1.302680e-48 | -8.615850e-47 |
| Msa0750880 | Msa1085260 | 0.821516 | 3.872948e-53 | -8.615850e-47 |
| Msa0795870 | Msa1085260 | 0.807148 | 5.822505e-50 | -8.615850e-47 |
| Msa0796530 | Msa1085260 | 0.803919 | 2.770389e-49 | -8.615850e-47 |
| Msa0127800 | Msa1085260 | 0.803206 | 3.894213e-49 | -8.615850e-47 |
| Msa0137840 | Msa1085260 | 0.811113 | 8.235904e-51 | -8.615850e-47 |
| Msa0157290 | Msa1085260 | 0.806847 | 6.741403e-50 | -8.615850e-47 |
| Msa0168500 | Msa1085260 | 0.806759 | 7.039265e-50 | -8.615850e-47 |
| Msa0170570 | Msa1085260 | 0.807321 | 5.352262e-50 | -8.615850e-47 |
| Msa0172450 | Msa1085260 | 0.826028 | 3.395839e-54 | -8.615850e-47 |
| Msa0183050 | Msa1085260 | 0.809445 | 1.885682e-50 | -8.615850e-47 |
| Msa0184040 | Msa1085260 | 0.815067 | 1.117662e-51 | -8.615850e-47 |
| Msa0205230 | Msa1085260 | 0.810147 | 1.331737e-50 | -8.615850e-47 |
| Msa0211150 | Msa1085260 | 0.805100 | 1.571553e-49 | -8.615850e-47 |
| Msa0222620 | Msa1085260 | 0.872113 | 4.080094e-67 | -8.615850e-47 |
| Msa0223080 | Msa1085260 | 0.882142 | 1.334867e-70 | -8.615850e-47 |
| Msa0225100 | Msa1085260 | 0.817716 | 2.855091e-52 | -8.615850e-47 |
| Msa0369400 | Msa1085260 | 0.803285 | 3.750591e-49 | -8.615850e-47 |
| Msa0371170 | Msa1085260 | 0.813917 | 2.008075e-51 | -8.615850e-47 |
| Msa0378720 | Msa1085260 | 0.806371 | 8.496349e-50 | -8.615850e-47 |
| Msa0395820 | Msa1085260 | 0.826762 | 2.271157e-54 | -8.615850e-47 |
| Msa0412270 | Msa1085260 | 0.807500 | 4.903984e-50 | -8.615850e-47 |
| Msa0412280 | Msa1085260 | 0.805329 | 1.407053e-49 | -8.615850e-47 |
| Msa0425170 | Msa1085260 | 0.803131 | 4.034804e-49 | -8.615850e-47 |
| Msa0428580 | Msa1085260 | 0.813574 | 2.388849e-51 | -8.615850e-47 |
| Msa0431720 | Msa1085260 | 0.804576 | 2.021571e-49 | -8.615850e-47 |
| Msa0439200 | Msa1085260 | 0.805217 | 1.485158e-49 | -8.615850e-47 |
| Msa0456880 | Msa1085260 | 0.814817 | 1.269921e-51 | -8.615850e-47 |
| Msa0463060 | Msa1085260 | 0.847521 | 1.097370e-59 | -8.615850e-47 |
| Msa0474940 | Msa1085260 | 0.800025 | 1.748605e-48 | -8.615850e-47 |
| Msa0475790 | Msa1085260 | 0.800069 | 1.713094e-48 | -8.615850e-47 |
| Msa0479170 | Msa1085260 | 0.818662 | 1.743448e-52 | -8.615850e-47 |
| Msa0479660 | Msa1085260 | 0.805846 | 1.096084e-49 | -8.615850e-47 |
| Msa0481530 | Msa1085260 | 0.831503 | 1.608510e-55 | -8.615850e-47 |
| Msa1004840 | Msa1085260 | 0.801334 | 9.458514e-49 | -8.615850e-47 |
| Msa1006840 | Msa1085260 | 0.830853 | 2.324369e-55 | -8.615850e-47 |
| Msa1012320 | Msa1085260 | 0.801226 | 9.950713e-49 | -8.615850e-47 |
| Msa1013400 | Msa1085260 | 0.831201 | 1.909209e-55 | -8.615850e-47 |
| Msa1019470 | Msa1085260 | 0.813263 | 2.796975e-51 | -8.615850e-47 |
| Msa1041670 | Msa1085260 | 0.810700 | 1.011970e-50 | -8.615850e-47 |
| Msa1075540 | Msa1085260 | 0.819289 | 1.256048e-52 | -8.615850e-47 |
| Msa1078150 | Msa1085260 | 0.814517 | 1.480204e-51 | -8.615850e-47 |
| Msa1078170 | Msa1085260 | 0.890874 | 6.639589e-74 | -8.615850e-47 |
| Msa1081330 | Msa1085260 | 0.807547 | 4.792079e-50 | -8.615850e-47 |
| Msa1085260 | Msa1093530 | 0.835799 | 1.359272e-56 | -8.615850e-47 |
| Msa1085260 | Msa1093840 | 0.852005 | 6.130847e-61 | -8.615850e-47 |
| Msa1085260 | Msa1098400 | 0.817622 | 2.997639e-52 | -8.615850e-47 |
| Msa1085260 | Msa1103990 | 0.813129 | 2.992474e-51 | -8.615850e-47 |
| Msa1085260 | Msa1123380 | 0.852643 | 4.034181e-61 | -8.615850e-47 |
| Msa1085260 | Msa1132840 | 0.807532 | 4.827476e-50 | -8.615850e-47 |
| Msa1085260 | Msa1142020 | 0.829498 | 4.976920e-55 | -8.615850e-47 |
| Msa1085260 | Msa1147900 | 0.807045 | 6.122003e-50 | -8.615850e-47 |
| Msa1085260 | Msa1147970 | 0.802787 | 4.752223e-49 | -8.615850e-47 |
| Msa1085260 | Msa1162780 | 0.803902 | 2.793426e-49 | -8.615850e-47 |
| Msa1085260 | Msa1166560 | 0.807606 | 4.656814e-50 | -8.615850e-47 |
| Msa1085260 | Msa1189070 | 0.817555 | 3.102742e-52 | -8.615850e-47 |
| Msa1085260 | Msa1193290 | 0.840928 | 6.470157e-58 | -8.615850e-47 |
| Msa1085260 | Msa1207270 | 0.830452 | 2.913775e-55 | -8.615850e-47 |
| Msa1085260 | Msa1209460 | 0.806017 | 1.008664e-49 | -8.615850e-47 |
| Msa1085260 | Msa1218450 | 0.801530 | 8.620326e-49 | -8.615850e-47 |
| Msa1085260 | Msa1218600 | 0.830389 | 3.018583e-55 | -8.615850e-47 |
| Msa1085260 | Msa1221050 | 0.829495 | 4.985173e-55 | -8.615850e-47 |
| Msa1085260 | Msa1221060 | 0.851512 | 8.456937e-61 | -8.615850e-47 |
| Msa1085260 | Msa1228200 | 0.803562 | 3.286132e-49 | -8.615850e-47 |
| Msa1085260 | Msa1251160 | 0.853440 | 2.385647e-61 | -8.615850e-47 |
| Msa1085260 | Msa1276660 | 0.809187 | 2.142170e-50 | -8.615850e-47 |
| Msa1085260 | Msa1284550 | 0.807141 | 5.844104e-50 | -8.615850e-47 |
| Msa1085260 | Msa1297290 | 0.800640 | 1.310797e-48 | -8.615850e-47 |
| Msa1085260 | Msa1303240 | 0.838865 | 2.231278e-57 | -8.615850e-47 |
| Msa1085260 | Msa1303250 | 0.852272 | 5.145858e-61 | -8.615850e-47 |
| Msa1085260 | Msa1305710 | 0.817470 | 3.243991e-52 | -8.615850e-47 |
| Msa1085260 | Msa1328710 | 0.803070 | 4.154207e-49 | -8.615850e-47 |
| Msa1085260 | Msa1329980 | 0.804528 | 2.069075e-49 | -8.615850e-47 |
| Msa1085260 | Msa1333480 | 0.805954 | 1.040163e-49 | -8.615850e-47 |
| Msa1085260 | Msa1342900 | 0.806284 | 8.862958e-50 | -8.615850e-47 |
| Msa1085260 | Msa1353060 | 0.816636 | 4.992927e-52 | -8.615850e-47 |
| Msa1085260 | Msa1353980 | 0.836222 | 1.061723e-56 | -8.615850e-47 |
| Msa1085260 | Msa1361400 | 0.832859 | 7.432593e-56 | -8.615850e-47 |
| Msa1085260 | Msa1361410 | 0.844458 | 7.467181e-59 | -8.615850e-47 |
| Msa1085260 | Msa1366600 | 0.800847 | 1.189391e-48 | -8.615850e-47 |
| Msa1085260 | Msa1373100 | 0.853327 | 2.571804e-61 | -8.615850e-47 |
| Msa1085260 | Msa1385000 | 0.800306 | 1.533041e-48 | -8.615850e-47 |
| Msa1085260 | Msa1402400 | 0.803900 | 2.795953e-49 | -8.615850e-47 |
| Msa1085260 | Msa1421570 | 0.821254 | 4.453196e-53 | -8.615850e-47 |
| Msa1085260 | Msa1422950 | 0.853462 | 2.352352e-61 | -8.615850e-47 |
| Msa1085260 | Msa1423350 | 0.805251 | 1.461199e-49 | -8.615850e-47 |
| Msa1085260 | Msa1425880 | 0.809970 | 1.454389e-50 | -8.615850e-47 |
| Msa1085260 | Msa1433390 | 0.811847 | 5.703783e-51 | -8.615850e-47 |
| Msa1085260 | Msa1440310 | 0.816438 | 5.529081e-52 | -8.615850e-47 |
| Msa1085260 | Msa1440880 | 0.859171 | 4.975160e-63 | -8.615850e-47 |
| Msa1085260 | Msa1446810 | 0.805233 | 1.473382e-49 | -8.615850e-47 |
| Msa1085260 | Msa1446820 | 0.879703 | 1.003426e-69 | -8.615850e-47 |
| Msa1085260 | Msa1451160 | 0.889506 | 2.278488e-73 | -8.615850e-47 |
| Msa1085260 | Msa1466540 | 0.814711 | 1.340414e-51 | -8.615850e-47 |
| Msa1085260 | Msa1466920 | 0.840833 | 6.851838e-58 | -8.615850e-47 |
| Msa0249200 | Msa1085260 | 0.815109 | 1.093793e-51 | -8.615850e-47 |
| Msa0254790 | Msa1085260 | 0.817191 | 3.747371e-52 | -8.615850e-47 |
| Msa0264150 | Msa1085260 | 0.833121 | 6.396541e-56 | -8.615850e-47 |
| Msa0266180 | Msa1085260 | 0.804937 | 1.699189e-49 | -8.615850e-47 |
| Msa0271530 | Msa1085260 | 0.810796 | 9.645390e-51 | -8.615850e-47 |
| Msa0287740 | Msa1085260 | 0.834338 | 3.176242e-56 | -8.615850e-47 |
| Msa0300270 | Msa1085260 | 0.807182 | 5.726755e-50 | -8.615850e-47 |
| Msa0300320 | Msa1085260 | 0.803516 | 3.358735e-49 | -8.615850e-47 |
| Msa0302160 | Msa1085260 | 0.881364 | 2.552407e-70 | -8.615850e-47 |
| Msa0302170 | Msa1085260 | 0.817711 | 2.861819e-52 | -8.615850e-47 |
| Msa0305610 | Msa1085260 | 0.800430 | 1.446821e-48 | -8.615850e-47 |
| Msa0309770 | Msa1085260 | 0.807618 | 4.628221e-50 | -8.615850e-47 |
| Msa0329320 | Msa1085260 | 0.800336 | 1.511914e-48 | -8.615850e-47 |
| Msa0337700 | Msa1085260 | 0.813490 | 2.492575e-51 | -8.615850e-47 |
| Msa0339250 | Msa1085260 | 0.864028 | 1.632696e-64 | -8.615850e-47 |
| Msa0339490 | Msa1085260 | 0.880645 | 4.629255e-70 | -8.615850e-47 |
| Msa0339500 | Msa1085260 | 0.817022 | 4.089758e-52 | -8.615850e-47 |
| Msa0502230 | Msa1085260 | 0.803738 | 3.020446e-49 | -8.615850e-47 |
| Msa0504210 | Msa1085260 | 0.846710 | 1.830473e-59 | -8.615850e-47 |
| Msa0529890 | Msa1085260 | 0.838494 | 2.782240e-57 | -8.615850e-47 |
| Msa0531180 | Msa1085260 | 0.811914 | 5.515996e-51 | -8.615850e-47 |
| Msa0534910 | Msa1085260 | 0.820561 | 6.426240e-53 | -8.615850e-47 |
| Msa0547720 | Msa1085260 | 0.854255 | 1.390053e-61 | -8.615850e-47 |
| Msa0551890 | Msa1085260 | 0.829237 | 5.760882e-55 | -8.615850e-47 |
| Msa0557070 | Msa1085260 | 0.815436 | 9.253051e-52 | -8.615850e-47 |
| Msa0557320 | Msa1085260 | 0.820346 | 7.200561e-53 | -8.615850e-47 |
| Msa0569200 | Msa1085260 | 0.818957 | 1.494569e-52 | -8.615850e-47 |
| Msa0571130 | Msa1085260 | 0.818024 | 2.431704e-52 | -8.615850e-47 |
| Msa0580370 | Msa1085260 | 0.800843 | 1.191468e-48 | -8.615850e-47 |
| Msa0581470 | Msa1085260 | 0.810886 | 9.222502e-51 | -8.615850e-47 |
| Msa0585560 | Msa1085260 | 0.834110 | 3.622800e-56 | -8.615850e-47 |
| Msa0590990 | Msa1085260 | 0.825734 | 3.988880e-54 | -8.615850e-47 |
| Msa0597790 | Msa1085260 | 0.802386 | 5.751372e-49 | -8.615850e-47 |
| Msa0876060 | Msa1085260 | 0.801224 | 9.960960e-49 | -8.615850e-47 |
| Msa0880140 | Msa1085260 | 0.806913 | 6.528207e-50 | -8.615850e-47 |
| Msa0897080 | Msa1085260 | 0.801722 | 7.873391e-49 | -8.615850e-47 |
| Msa0902760 | Msa1085260 | 0.835350 | 1.766399e-56 | -8.615850e-47 |
| Msa0941190 | Msa1085260 | 0.848226 | 7.016689e-60 | -8.615850e-47 |
| Msa0963270 | Msa1085260 | 0.800616 | 1.325610e-48 | -8.615850e-47 |
| Msa0964310 | Msa1085260 | 0.810978 | 8.810599e-51 | -8.615850e-47 |
| Msa0967870 | Msa1085260 | 0.825779 | 3.891494e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1085260 | MtrunA17_Chr4g0072411 | 100.000 | 103 | 0 | 0 | 1 | 103 | 59 | 161 | 5.39e-69 | 203 |
| Msa1085260 | MtrunA17_Chr5g0425951 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1085260 | MtrunA17_Chr4g0030431 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1085260 | MtrunA17_Chr3g0100121 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1085260 | MtrunA17_Chr3g0113681 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1085260 | MtrunA17_Chr2g0327641 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1085260 | MtrunA17_Chr3g0103351 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1085260 | MtrunA17_Chr8g0352791 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1085260 | MtrunA17_Chr3g0103821 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1085260 | MtrunA17_Chr3g0104031 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1085260 | MtrunA17_Chr7g0225731 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1085260 | MtrunA17_Chr3g0103801 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1085260 | MtrunA17_Chr3g0103881 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa1085260 | MtrunA17_Chr7g0262791 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1085260 | AT3G53730.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1085260 | AT1G07820.2 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1085260 | AT1G07820.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1085260 | AT5G59690.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1085260 | AT3G46320.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1085260 | AT2G28740.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1085260 | AT1G07660.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1085260 | AT3G45930.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1085260 | AT5G59970.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1085260 | AT5G59970.2 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa1085260 | AT1G07660.2 | 83.495 | 103 | 0 | 1 | 1 | 103 | 1 | 86 | 4.52e-51 | 155 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGTCAGGCCGTGGAAA+GGG | 0.388540 | 7_2:+85769636 | None:intergenic |
| AGACAAGGAAGAACCCTCTA+TGG | 0.431401 | 7_2:+85769915 | Msa1085260:CDS |
| AGGTGTATTGAAGATCTTCT+TGG | 0.432837 | 7_2:+85769806 | Msa1085260:CDS |
| GGAAGAACCCTCTATGGTTT+CGG | 0.438131 | 7_2:+85769921 | Msa1085260:CDS |
| CAATCGACGAATAGCAGGTT+TGG | 0.439411 | 7_2:-85769730 | None:intergenic |
| ATCAACCTCCGAAACCATAG+AGG | 0.481091 | 7_2:-85769929 | None:intergenic |
| CTTGCCAATCGACGAATAGC+AGG | 0.487494 | 7_2:-85769735 | None:intergenic |
| GAAGATCTTCAATACACCTC+TGG | 0.507768 | 7_2:-85769802 | None:intergenic |
| CTCGCGAAAATGTCAGGCCG+TGG | 0.510433 | 7_2:+85769630 | None:intergenic |
| TTACATACACTGAGCATGCT+AGG | 0.520723 | 7_2:+85769850 | Msa1085260:CDS |
| AAATGTCAGGCCGTGGAAAG+GGG | 0.522293 | 7_2:+85769637 | None:intergenic |
| AGTTCTTCGTGATAACATCC+AGG | 0.522451 | 7_2:+85769701 | Msa1085260:CDS |
| GAGGAAGACGGTGACAGCCA+TGG | 0.546349 | 7_2:+85769872 | Msa1085260:CDS |
| GAAAATGTCAGGCCGTGGAA+AGG | 0.547374 | 7_2:+85769635 | None:intergenic |
| CAAACCTGCTATTCGTCGAT+TGG | 0.553041 | 7_2:+85769731 | Msa1085260:CDS |
| AATGTCAGGCCGTGGAAAGG+GGG | 0.554279 | 7_2:+85769638 | None:intergenic |
| GGTAAGGGTCTTGGAAAGGG+AGG | 0.556685 | 7_2:+85769660 | Msa1085260:CDS |
| AGGGAGGTGCAAAGAGACAT+AGG | 0.568952 | 7_2:+85769676 | Msa1085260:CDS |
| GTCTATGCTTTGAAGAGACA+AGG | 0.570048 | 7_2:+85769900 | Msa1085260:CDS |
| CGTCGATTGGCAAGAAGAGG+CGG | 0.578876 | 7_2:+85769744 | Msa1085260:CDS |
| GGCGGTGTTAAGAGAATCAG+TGG | 0.595979 | 7_2:+85769762 | Msa1085260:CDS |
| ATGTCAGGCCGTGGAAAGGG+GGG | 0.596640 | 7_2:+85769639 | Msa1085260:CDS |
| TAGCAGGTTTGGTGATACCC+TGG | 0.596796 | 7_2:-85769719 | None:intergenic |
| AATCAGTGGATTGATCTATG+AGG | 0.599604 | 7_2:+85769776 | Msa1085260:CDS |
| ATTCGTCGATTGGCAAGAAG+AGG | 0.622105 | 7_2:+85769741 | Msa1085260:CDS |
| CAAAGCATAGACAACATCCA+TGG | 0.629842 | 7_2:-85769889 | None:intergenic |
| AGAACCCTCTATGGTTTCGG+AGG | 0.630900 | 7_2:+85769924 | Msa1085260:CDS |
| GTTCTTCGTGATAACATCCA+GGG | 0.641996 | 7_2:+85769702 | Msa1085260:CDS |
| TCAACCTCCGAAACCATAGA+GGG | 0.666233 | 7_2:-85769928 | None:intergenic |
| TGAGCATGCTAGGAGGAAGA+CGG | 0.671956 | 7_2:+85769860 | Msa1085260:CDS |
| TTGATCTATGAGGAAACCAG+AGG | 0.700822 | 7_2:+85769786 | Msa1085260:CDS |
| CATACACTGAGCATGCTAGG+AGG | 0.707859 | 7_2:+85769853 | Msa1085260:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCAGTGGATTGATCTATG+AGG | + | chr7_2:85769776-85769795 | Msa1085260:CDS | 35.0% |
| AGGTGTATTGAAGATCTTCT+TGG | + | chr7_2:85769806-85769825 | Msa1085260:CDS | 35.0% | |
| AGTTCTTCGTGATAACATCC+AGG | + | chr7_2:85769701-85769720 | Msa1085260:CDS | 40.0% | |
| GTTCTTCGTGATAACATCCA+GGG | + | chr7_2:85769702-85769721 | Msa1085260:CDS | 40.0% | |
| TTGATCTATGAGGAAACCAG+AGG | + | chr7_2:85769786-85769805 | Msa1085260:CDS | 40.0% | |
| GAAGATCTTCAATACACCTC+TGG | - | chr7_2:85769805-85769824 | None:intergenic | 40.0% | |
| TTACATACACTGAGCATGCT+AGG | + | chr7_2:85769850-85769869 | Msa1085260:CDS | 40.0% | |
| CAAAGCATAGACAACATCCA+TGG | - | chr7_2:85769892-85769911 | None:intergenic | 40.0% | |
| !! | GTCTATGCTTTGAAGAGACA+AGG | + | chr7_2:85769900-85769919 | Msa1085260:CDS | 40.0% |
| CAATCGACGAATAGCAGGTT+TGG | - | chr7_2:85769733-85769752 | None:intergenic | 45.0% | |
| CAAACCTGCTATTCGTCGAT+TGG | + | chr7_2:85769731-85769750 | Msa1085260:CDS | 45.0% | |
| ! | ATTCGTCGATTGGCAAGAAG+AGG | + | chr7_2:85769741-85769760 | Msa1085260:CDS | 45.0% |
| AGACAAGGAAGAACCCTCTA+TGG | + | chr7_2:85769915-85769934 | Msa1085260:CDS | 45.0% | |
| GGAAGAACCCTCTATGGTTT+CGG | + | chr7_2:85769921-85769940 | Msa1085260:CDS | 45.0% | |
| TCAACCTCCGAAACCATAGA+GGG | - | chr7_2:85769931-85769950 | None:intergenic | 45.0% | |
| ! | AGGGAGGTGCAAAGAGACAT+AGG | + | chr7_2:85769676-85769695 | Msa1085260:CDS | 50.0% |
| !! | TAGCAGGTTTGGTGATACCC+TGG | - | chr7_2:85769722-85769741 | None:intergenic | 50.0% |
| CTTGCCAATCGACGAATAGC+AGG | - | chr7_2:85769738-85769757 | None:intergenic | 50.0% | |
| ! | GGCGGTGTTAAGAGAATCAG+TGG | + | chr7_2:85769762-85769781 | Msa1085260:CDS | 50.0% |
| CATACACTGAGCATGCTAGG+AGG | + | chr7_2:85769853-85769872 | Msa1085260:CDS | 50.0% | |
| TGAGCATGCTAGGAGGAAGA+CGG | + | chr7_2:85769860-85769879 | Msa1085260:CDS | 50.0% | |
| AGAACCCTCTATGGTTTCGG+AGG | + | chr7_2:85769924-85769943 | Msa1085260:CDS | 50.0% | |
| GGGGGTAAGGGTCTTGGAAA+GGG | + | chr7_2:85769657-85769676 | Msa1085260:CDS | 55.0% | |
| GGTAAGGGTCTTGGAAAGGG+AGG | + | chr7_2:85769660-85769679 | Msa1085260:CDS | 55.0% | |
| ! | CGTCGATTGGCAAGAAGAGG+CGG | + | chr7_2:85769744-85769763 | Msa1085260:CDS | 55.0% |
| ATGTCAGGCCGTGGAAAGGG+GGG | + | chr7_2:85769639-85769658 | Msa1085260:CDS | 60.0% | |
| GACCCTTACCCCCCTTTCCA+CGG | - | chr7_2:85769650-85769669 | None:intergenic | 60.0% | |
| GGAAAGGGGGGTAAGGGTCT+TGG | + | chr7_2:85769651-85769670 | Msa1085260:CDS | 60.0% | |
| GGGGGGTAAGGGTCTTGGAA+AGG | + | chr7_2:85769656-85769675 | Msa1085260:CDS | 60.0% | |
| GAGGAAGACGGTGACAGCCA+TGG | + | chr7_2:85769872-85769891 | Msa1085260:CDS | 60.0% | |
| AGGCCGTGGAAAGGGGGGTA+AGG | + | chr7_2:85769644-85769663 | Msa1085260:CDS | 65.0% | |
| GGCCGTGGAAAGGGGGGTAA+GGG | + | chr7_2:85769645-85769664 | Msa1085260:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 85769639 | 85769950 | 85769639 | ID=Msa1085260;Name=Msa1085260 |
| chr7_2 | mRNA | 85769639 | 85769950 | 85769639 | ID=Msa1085260-mRNA-1;Parent=Msa1085260;Name=Msa1085260-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|103 |
| chr7_2 | exon | 85769639 | 85769950 | 85769639 | ID=Msa1085260-mRNA-1:exon:23190;Parent=Msa1085260-mRNA-1 |
| chr7_2 | CDS | 85769639 | 85769950 | 85769639 | ID=Msa1085260-mRNA-1:cds;Parent=Msa1085260-mRNA-1 |
| Gene Sequence |
| Protein sequence |
