Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0035140 | Msa0460780 | 0.814356 | 1.606130e-51 | -8.615850e-47 |
| Msa0037740 | Msa0460780 | 0.822742 | 2.013217e-53 | -8.615850e-47 |
| Msa0056180 | Msa0460780 | 0.816502 | 5.351072e-52 | -8.615850e-47 |
| Msa0077560 | Msa0460780 | 0.817922 | 2.564325e-52 | -8.615850e-47 |
| Msa0115550 | Msa0460780 | 0.807525 | 4.844409e-50 | -8.615850e-47 |
| Msa0172480 | Msa0460780 | 0.831974 | 1.231175e-55 | -8.615850e-47 |
| Msa0373500 | Msa0460780 | 0.810953 | 8.918231e-51 | -8.615850e-47 |
| Msa0380490 | Msa0460780 | 0.815484 | 9.028412e-52 | -8.615850e-47 |
| Msa0404770 | Msa0460780 | 0.800703 | 1.272442e-48 | -8.615850e-47 |
| Msa0412270 | Msa0460780 | 0.820925 | 5.301786e-53 | -8.615850e-47 |
| Msa0414880 | Msa0460780 | 0.815720 | 7.999577e-52 | -8.615850e-47 |
| Msa0430000 | Msa0460780 | 0.837100 | 6.342377e-57 | -8.615850e-47 |
| Msa0432010 | Msa0460780 | 0.816766 | 4.668307e-52 | -8.615850e-47 |
| Msa0456670 | Msa0460780 | 0.821926 | 3.114134e-53 | -8.615850e-47 |
| Msa0459740 | Msa0460780 | 0.802563 | 5.287225e-49 | -8.615850e-47 |
| Msa0460780 | Msa0468620 | 0.807704 | 4.438709e-50 | -8.615850e-47 |
| Msa0460780 | Msa0468700 | 0.802750 | 4.836804e-49 | -8.615850e-47 |
| Msa0460780 | Msa0481890 | 0.847208 | 1.337629e-59 | -8.615850e-47 |
| Msa0460780 | Msa0500570 | 0.829796 | 4.212243e-55 | -8.615850e-47 |
| Msa0460780 | Msa0503990 | 0.809885 | 1.516959e-50 | -8.615850e-47 |
| Msa0460780 | Msa0507020 | 0.968432 | 1.143251e-128 | -8.615850e-47 |
| Msa0460780 | Msa0514500 | 0.807574 | 4.729439e-50 | -8.615850e-47 |
| Msa0460780 | Msa0520870 | 0.810489 | 1.124023e-50 | -8.615850e-47 |
| Msa0460780 | Msa0548000 | 0.814620 | 1.404346e-51 | -8.615850e-47 |
| Msa0460780 | Msa0647280 | 0.811140 | 8.125576e-51 | -8.615850e-47 |
| Msa0460780 | Msa0686800 | 0.805855 | 1.091183e-49 | -8.615850e-47 |
| Msa0460780 | Msa0709440 | 0.849294 | 3.546469e-60 | -8.615850e-47 |
| Msa0460780 | Msa0758590 | 0.811779 | 5.902273e-51 | -8.615850e-47 |
| Msa0460780 | Msa0780910 | 0.815629 | 8.382175e-52 | -8.615850e-47 |
| Msa0460780 | Msa0821160 | 0.808664 | 2.771278e-50 | -8.615850e-47 |
| Msa0460780 | Msa0909760 | 0.820856 | 5.497606e-53 | -8.615850e-47 |
| Msa0460780 | Msa1033920 | 0.836793 | 7.599649e-57 | -8.615850e-47 |
| Msa0460780 | Msa1037540 | 0.807822 | 4.190028e-50 | -8.615850e-47 |
| Msa0460780 | Msa1078150 | 0.824453 | 8.007100e-54 | -8.615850e-47 |
| Msa0460780 | Msa1084530 | 0.841770 | 3.884701e-58 | -8.615850e-47 |
| Msa0460780 | Msa1116660 | 0.804520 | 2.076999e-49 | -8.615850e-47 |
| Msa0460780 | Msa1125820 | 0.843111 | 1.712919e-58 | -8.615850e-47 |
| Msa0460780 | Msa1125990 | 0.843089 | 1.736063e-58 | -8.615850e-47 |
| Msa0460780 | Msa1130420 | 0.812203 | 4.770971e-51 | -8.615850e-47 |
| Msa0460780 | Msa1162880 | 0.816494 | 5.373357e-52 | -8.615850e-47 |
| Msa0460780 | Msa1171290 | 0.847002 | 1.522695e-59 | -8.615850e-47 |
| Msa0460780 | Msa1218820 | 0.803382 | 3.579587e-49 | -8.615850e-47 |
| Msa0460780 | Msa1368750 | 0.813760 | 2.174582e-51 | -8.615850e-47 |
| Msa0460780 | Msa1374790 | 0.813742 | 2.194129e-51 | -8.615850e-47 |
| Msa0460780 | Msa1421570 | 0.832539 | 8.925187e-56 | -8.615850e-47 |
| Msa0460780 | Msa1440880 | 0.801323 | 9.504529e-49 | -8.615850e-47 |
| Msa0460780 | Msa1446100 | 0.816708 | 4.811855e-52 | -8.615850e-47 |
| Msa0460780 | Msa1447170 | 0.828192 | 1.030733e-54 | -8.615850e-47 |
| Msa0460780 | Msa1451820 | 0.813697 | 2.244581e-51 | -8.615850e-47 |
| Msa0460780 | Msa1454010 | 0.803994 | 2.672758e-49 | -8.615850e-47 |
| Msa0363990 | Msa0460780 | 0.817321 | 3.503685e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCGCCAATCGACGAATTGC+AGG | 0.267346 | 3_3:-58394485 | None:intergenic |
| GGAAGAACCCTCTATGGATT+TGG | 0.370047 | 3_3:+58394671 | Msa0460780:three_prime_UTR |
| AGACAAGGAAGAACCCTCTA+TGG | 0.396310 | 3_3:+58394665 | Msa0460780:three_prime_UTR |
| AGGTGTCTTGAAGATCTTCT+TGG | 0.405705 | 3_3:+58394556 | Msa0460780:three_prime_UTR |
| GGGAGGTAAGGGACTTGGAA+AGG | 0.449159 | 3_3:+58394406 | Msa0460780:CDS |
| GGAGGTAAGGGACTTGGAAA+GGG | 0.467307 | 3_3:+58394407 | Msa0460780:CDS |
| GGAAAGGGAGGTAAGGGACT+TGG | 0.470633 | 3_3:+58394401 | Msa0460780:CDS |
| TTCAACCTCCAAATCCATAG+AGG | 0.476286 | 3_3:-58394679 | None:intergenic |
| CTGTTGAAAATGTCAGGTCG+TGG | 0.487154 | 3_3:+58394380 | None:intergenic |
| CAATCGACGAATTGCAGGCT+TGG | 0.491738 | 3_3:-58394480 | None:intergenic |
| AGGTCGTGGAAAGGGAGGTA+AGG | 0.492819 | 3_3:+58394394 | Msa0460780:CDS |
| AGTTCTCCGCGATAACATCC+AGG | 0.504812 | 3_3:+58394451 | Msa0460780:CDS |
| AGAACCCTCTATGGATTTGG+AGG | 0.508817 | 3_3:+58394674 | Msa0460780:three_prime_UTR |
| GAAGATCTTCAAGACACCTC+TGG | 0.510902 | 3_3:-58394552 | None:intergenic |
| CAAGCCTGCAATTCGTCGAT+TGG | 0.511374 | 3_3:+58394481 | Msa0460780:CDS |
| TTACATACACTGAGCATGCT+AGG | 0.520723 | 3_3:+58394600 | Msa0460780:three_prime_UTR |
| GAGGAAGACGGTGACAGCCA+TGG | 0.546349 | 3_3:+58394622 | Msa0460780:three_prime_UTR |
| AGGGAGGTGCAAAGAGGCAT+AGG | 0.547891 | 3_3:+58394426 | Msa0460780:CDS |
| AAAATGTCAGGTCGTGGAAA+GGG | 0.548959 | 3_3:+58394386 | None:intergenic |
| GGTAAGGGACTTGGAAAGGG+AGG | 0.560743 | 3_3:+58394410 | Msa0460780:CDS |
| GGTCGTGGAAAGGGAGGTAA+GGG | 0.564245 | 3_3:+58394395 | Msa0460780:CDS |
| GTCTATGCTTTGAAGAGACA+AGG | 0.570048 | 3_3:+58394650 | Msa0460780:three_prime_UTR |
| GAAAATGTCAGGTCGTGGAA+AGG | 0.584382 | 3_3:+58394385 | None:intergenic |
| CGTCGATTGGCGAGAAGAGG+CGG | 0.593362 | 3_3:+58394494 | Msa0460780:CDS |
| GGCGGTGTTAAGAGAATCAG+TGG | 0.595979 | 3_3:+58394512 | Msa0460780:CDS |
| ATTCGTCGATTGGCGAGAAG+AGG | 0.604517 | 3_3:+58394491 | Msa0460780:CDS |
| TTGCAGGCTTGGTGATACCC+TGG | 0.613296 | 3_3:-58394469 | None:intergenic |
| CAAAGCATAGACAACATCCA+TGG | 0.629842 | 3_3:-58394639 | None:intergenic |
| TCAACCTCCAAATCCATAGA+GGG | 0.633476 | 3_3:-58394678 | None:intergenic |
| ATGTCAGGTCGTGGAAAGGG+AGG | 0.643397 | 3_3:+58394389 | None:intergenic |
| TGATACCCTGGATGTTATCG+CGG | 0.665173 | 3_3:-58394457 | None:intergenic |
| TGAGCATGCTAGGAGGAAGA+CGG | 0.671956 | 3_3:+58394610 | Msa0460780:three_prime_UTR |
| GTTCTCCGCGATAACATCCA+GGG | 0.672360 | 3_3:+58394452 | Msa0460780:CDS |
| ATCTTAAAGAACAATTACAC+AGG | 0.681255 | 3_3:-58394745 | None:intergenic |
| TTGGAAAGGGAGGTGCAAAG+AGG | 0.683014 | 3_3:+58394420 | Msa0460780:CDS |
| TTGATCTATGAAGAAACCAG+AGG | 0.704889 | 3_3:+58394536 | Msa0460780:exon |
| CATACACTGAGCATGCTAGG+AGG | 0.707859 | 3_3:+58394603 | Msa0460780:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCTTAAAGAACAATTACAC+AGG | - | chr3_3:58394748-58394767 | None:intergenic | 25.0% |
| TTGATCTATGAAGAAACCAG+AGG | + | chr3_3:58394536-58394555 | Msa0460780:exon | 35.0% | |
| AGGTGTCTTGAAGATCTTCT+TGG | + | chr3_3:58394556-58394575 | Msa0460780:three_prime_UTR | 40.0% | |
| TTACATACACTGAGCATGCT+AGG | + | chr3_3:58394600-58394619 | Msa0460780:three_prime_UTR | 40.0% | |
| CAAAGCATAGACAACATCCA+TGG | - | chr3_3:58394642-58394661 | None:intergenic | 40.0% | |
| !! | GTCTATGCTTTGAAGAGACA+AGG | + | chr3_3:58394650-58394669 | Msa0460780:three_prime_UTR | 40.0% |
| TCAACCTCCAAATCCATAGA+GGG | - | chr3_3:58394681-58394700 | None:intergenic | 40.0% | |
| TTCAACCTCCAAATCCATAG+AGG | - | chr3_3:58394682-58394701 | None:intergenic | 40.0% | |
| TGATACCCTGGATGTTATCG+CGG | - | chr3_3:58394460-58394479 | None:intergenic | 45.0% | |
| GAAGATCTTCAAGACACCTC+TGG | - | chr3_3:58394555-58394574 | None:intergenic | 45.0% | |
| AGACAAGGAAGAACCCTCTA+TGG | + | chr3_3:58394665-58394684 | Msa0460780:three_prime_UTR | 45.0% | |
| GGAAGAACCCTCTATGGATT+TGG | + | chr3_3:58394671-58394690 | Msa0460780:three_prime_UTR | 45.0% | |
| AGAACCCTCTATGGATTTGG+AGG | + | chr3_3:58394674-58394693 | Msa0460780:three_prime_UTR | 45.0% | |
| GGAGGTAAGGGACTTGGAAA+GGG | + | chr3_3:58394407-58394426 | Msa0460780:CDS | 50.0% | |
| ! | TTGGAAAGGGAGGTGCAAAG+AGG | + | chr3_3:58394420-58394439 | Msa0460780:CDS | 50.0% |
| AGTTCTCCGCGATAACATCC+AGG | + | chr3_3:58394451-58394470 | Msa0460780:CDS | 50.0% | |
| GTTCTCCGCGATAACATCCA+GGG | + | chr3_3:58394452-58394471 | Msa0460780:CDS | 50.0% | |
| CAATCGACGAATTGCAGGCT+TGG | - | chr3_3:58394483-58394502 | None:intergenic | 50.0% | |
| CAAGCCTGCAATTCGTCGAT+TGG | + | chr3_3:58394481-58394500 | Msa0460780:CDS | 50.0% | |
| ! | ATTCGTCGATTGGCGAGAAG+AGG | + | chr3_3:58394491-58394510 | Msa0460780:CDS | 50.0% |
| ! | GGCGGTGTTAAGAGAATCAG+TGG | + | chr3_3:58394512-58394531 | Msa0460780:CDS | 50.0% |
| CATACACTGAGCATGCTAGG+AGG | + | chr3_3:58394603-58394622 | Msa0460780:three_prime_UTR | 50.0% | |
| TGAGCATGCTAGGAGGAAGA+CGG | + | chr3_3:58394610-58394629 | Msa0460780:three_prime_UTR | 50.0% | |
| AGGTCGTGGAAAGGGAGGTA+AGG | + | chr3_3:58394394-58394413 | Msa0460780:CDS | 55.0% | |
| GGTCGTGGAAAGGGAGGTAA+GGG | + | chr3_3:58394395-58394414 | Msa0460780:CDS | 55.0% | |
| GGAAAGGGAGGTAAGGGACT+TGG | + | chr3_3:58394401-58394420 | Msa0460780:CDS | 55.0% | |
| GGGAGGTAAGGGACTTGGAA+AGG | + | chr3_3:58394406-58394425 | Msa0460780:CDS | 55.0% | |
| GGTAAGGGACTTGGAAAGGG+AGG | + | chr3_3:58394410-58394429 | Msa0460780:CDS | 55.0% | |
| ! | AGGGAGGTGCAAAGAGGCAT+AGG | + | chr3_3:58394426-58394445 | Msa0460780:CDS | 55.0% |
| !! | TTGCAGGCTTGGTGATACCC+TGG | - | chr3_3:58394472-58394491 | None:intergenic | 55.0% |
| CTCGCCAATCGACGAATTGC+AGG | - | chr3_3:58394488-58394507 | None:intergenic | 55.0% | |
| ! | CGTCGATTGGCGAGAAGAGG+CGG | + | chr3_3:58394494-58394513 | Msa0460780:CDS | 60.0% |
| GAGGAAGACGGTGACAGCCA+TGG | + | chr3_3:58394622-58394641 | Msa0460780:three_prime_UTR | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 58394393 | 58394787 | 58394393 | ID=Msa0460780;Name=Msa0460780 |
| chr3_3 | mRNA | 58394393 | 58394787 | 58394393 | ID=Msa0460780-mRNA-1;Parent=Msa0460780;Name=Msa0460780-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0|1|0.5|1|0|0|2|227|48 |
| chr3_3 | exon | 58394393 | 58394702 | 58394393 | ID=Msa0460780-mRNA-1:exon:12146;Parent=Msa0460780-mRNA-1 |
| chr3_3 | exon | 58394724 | 58394787 | 58394724 | ID=Msa0460780-mRNA-1:exon:12147;Parent=Msa0460780-mRNA-1 |
| chr3_3 | CDS | 58394393 | 58394539 | 58394393 | ID=Msa0460780-mRNA-1:cds;Parent=Msa0460780-mRNA-1 |
| chr3_3 | three_prime_UTR | 58394540 | 58394702 | 58394540 | ID=Msa0460780-mRNA-1:three_prime_utr;Parent=Msa0460780-mRNA-1 |
| chr3_3 | three_prime_UTR | 58394724 | 58394787 | 58394724 | ID=Msa0460780-mRNA-1:three_prime_utr;Parent=Msa0460780-mRNA-1 |
| Gene Sequence |
| Protein sequence |
