AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1146120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1146120 MtrunA17_Chr3g0101871 55.828 163 72 0 1 163 328 490 1.23e-55 189
Msa1146120 MtrunA17_Chr7g0269651 41.772 158 91 1 6 162 383 540 9.18e-35 130
Msa1146120 MtrunA17_Chr1g0206491 43.151 146 82 1 10 154 303 448 2.83e-32 122
Msa1146120 MtrunA17_Chr1g0173671 39.869 153 87 3 13 162 380 530 4.32e-29 113
Msa1146120 MtrunA17_Chr5g0400991 40.678 118 69 1 46 162 3 120 2.59e-24 94.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 53 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGTTCCATGCCAGTTATT+TGG 0.210260 7_4:+23241012 Msa1146120:CDS
CTCTCCATATGTGAAAATTA+AGG 0.247996 7_4:-23241047 None:intergenic
GCTTTCAAGAGGAGCGTATA+AGG 0.273943 7_4:+23242178 Msa1146120:three_prime_UTR
GAGTTCCATGCCAGTTATTT+GGG 0.280817 7_4:+23241013 Msa1146120:CDS
GGTTGAACAGGCTTCATAAA+AGG 0.294996 7_4:+23241969 Msa1146120:intron
ATGTGAAAATTAAGGGATAT+TGG 0.328424 7_4:-23241039 None:intergenic
GATTCTTAGTCTGCAGTTCC+AGG 0.330292 7_4:-23242057 None:intergenic
TGAACTCTTTATTATTTCAT+TGG 0.347321 7_4:-23242212 None:intergenic
TACGCACTTGTAGCTTTATC+TGG 0.348177 7_4:+23242311 Msa1146120:three_prime_UTR
AGTCTATATCTCCGTCAATC+AGG 0.348760 7_4:-23240931 None:intergenic
GCACAACCGTTTGACTATAA+AGG 0.385568 7_4:+23241126 Msa1146120:CDS
AGGTATCTTATTAAGATATA+AGG 0.386097 7_4:+23241084 Msa1146120:CDS
AGGCATGGAGGATTTAGCTT+CGG 0.396449 7_4:-23242017 None:intergenic
TCTCCATATGTGAAAATTAA+GGG 0.399604 7_4:-23241046 None:intergenic
GGCATGGAGGATTTAGCTTC+GGG 0.401556 7_4:-23242016 None:intergenic
ATTATCCTTCACGGACAATC+AGG 0.411425 7_4:+23240971 Msa1146120:CDS
CTTTCAAGAGGAGCGTATAA+GGG 0.424392 7_4:+23242179 Msa1146120:three_prime_UTR
ATATTGGAAACCCAAATAAC+TGG 0.425409 7_4:-23241023 None:intergenic
AGTCATAGCCTCACATAGTC+TGG 0.427674 7_4:-23241173 None:intergenic
AACAGGCTTCATAAAAGGTT+GGG 0.454313 7_4:+23241974 Msa1146120:intron
GAAGTAGCTGCCCTGATTGA+CGG 0.458398 7_4:+23240920 Msa1146120:CDS
TCCACTTGGATGCCTCTACC+TGG 0.474448 7_4:+23242039 Msa1146120:CDS
ATTTCAGATCGGAACAATGA+TGG 0.486958 7_4:+23240869 Msa1146120:CDS
AAATATAATTGATTGTAGCT+TGG 0.494397 7_4:+23242262 Msa1146120:three_prime_UTR
GCCATGCCTTTATAGTCAAA+CGG 0.503688 7_4:-23241132 None:intergenic
TAGTCTGCAGTTCCAGGTAG+AGG 0.507256 7_4:-23242051 None:intergenic
GAACAGGCTTCATAAAAGGT+TGG 0.508152 7_4:+23241973 Msa1146120:intron
CGTAGAGATATTATCCTTCA+CGG 0.514940 7_4:+23240962 Msa1146120:CDS
ATCAAATAAGGGTATTGATG+AGG 0.537308 7_4:+23240730 Msa1146120:CDS
AAATCCTCCATGCCTCCACT+TGG 0.537804 7_4:+23242025 Msa1146120:CDS
CACTTGTAGCTTTATCTGGT+GGG 0.542071 7_4:+23242315 Msa1146120:three_prime_UTR
AATTGAAGCTTATTTCAGAT+CGG 0.544606 7_4:+23240858 Msa1146120:CDS
GGAAACCCAAATAACTGGCA+TGG 0.552341 7_4:-23241018 None:intergenic
GAGGCATCCAAGTGGAGGCA+TGG 0.552768 7_4:-23242032 None:intergenic
GTCTATATCTCCGTCAATCA+GGG 0.565246 7_4:-23240930 None:intergenic
GCATGCACAGGCTTTCAGAA+TGG 0.566296 7_4:+23240796 Msa1146120:CDS
GCAGCTACTTCTGACACAGT+TGG 0.567179 7_4:-23240908 None:intergenic
TCAAATAAGGGTATTGATGA+GGG 0.572285 7_4:+23240731 Msa1146120:CDS
TCTTGAAAGCTATATATACA+TGG 0.579030 7_4:-23242165 None:intergenic
TTATATCTTAATAAGATACC+TGG 0.582499 7_4:-23241082 None:intergenic
GGAGAGGATGGATATCGTCC+AGG 0.584813 7_4:+23241064 Msa1146120:CDS
GCACTTGTAGCTTTATCTGG+TGG 0.590034 7_4:+23242314 Msa1146120:three_prime_UTR
TTTCAAGAGGAGCGTATAAG+GGG 0.598545 7_4:+23242180 Msa1146120:three_prime_UTR
TGCAAACAATGTGCATGCAC+AGG 0.607446 7_4:+23240784 Msa1146120:CDS
ACCGTTTGACTATAAAGGCA+TGG 0.623638 7_4:+23241131 Msa1146120:CDS
ATGTATATATAGCTTTCAAG+AGG 0.628328 7_4:+23242167 Msa1146120:three_prime_UTR
AGGTAGAGGCATCCAAGTGG+AGG 0.638147 7_4:-23242037 None:intergenic
ACTAATGTCCAGACTATGTG+AGG 0.652666 7_4:+23241165 Msa1146120:CDS
TCCAGGTAGAGGCATCCAAG+TGG 0.667239 7_4:-23242040 None:intergenic
GTTCACCTGATTGTCCGTGA+AGG 0.672884 7_4:-23240976 None:intergenic
TTCAAGAGGAGCGTATAAGG+GGG 0.690215 7_4:+23242181 Msa1146120:three_prime_UTR
AAGGATAATATCTCTACGCG+AGG 0.691672 7_4:-23240957 None:intergenic
GCATCCAAGTGGAGGCATGG+AGG 0.691733 7_4:-23242029 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGGTAAATTTTATTTTGAA+AGG - chr7_4:23241764-23241783 None:intergenic 15.0%
!! AAATAAAATTTACCTTAATG+TGG + chr7_4:23241768-23241787 Msa1146120:intron 15.0%
!!! TCTTCTAAATATTTTTCATA+GGG - chr7_4:23242346-23242365 None:intergenic 15.0%
!!! TTCTTCTAAATATTTTTCAT+AGG - chr7_4:23242347-23242366 None:intergenic 15.0%
!!! TATACTTAGATTTTAATTGT+AGG + chr7_4:23242370-23242389 Msa1146120:three_prime_UTR 15.0%
!!! AAGGTAAATTTTATTTTGAA+AGG - chr7_4:23241764-23241783 None:intergenic 15.0%
!! AAATAAAATTTACCTTAATG+TGG + chr7_4:23241768-23241787 Msa1146120:intron 15.0%
!!! TCTTCTAAATATTTTTCATA+GGG - chr7_4:23242346-23242365 None:intergenic 15.0%
!!! TTCTTCTAAATATTTTTCAT+AGG - chr7_4:23242347-23242366 None:intergenic 15.0%
!!! TATACTTAGATTTTAATTGT+AGG + chr7_4:23242370-23242389 Msa1146120:three_prime_UTR 15.0%
!!! CAATTTTACAGATCAAATAA+GGG + chr7_4:23240719-23240738 Msa1146120:CDS 20.0%
!! TTATATCTTAATAAGATACC+TGG - chr7_4:23241085-23241104 None:intergenic 20.0%
!! AGGTATCTTATTAAGATATA+AGG + chr7_4:23241084-23241103 Msa1146120:CDS 20.0%
!! TAAAGATAAATTTGGTCATT+TGG - chr7_4:23241344-23241363 None:intergenic 20.0%
!! AGACATCATAAAGATAAATT+TGG - chr7_4:23241352-23241371 None:intergenic 20.0%
!! TAATTATTGTTTCCTCTATA+TGG + chr7_4:23241439-23241458 Msa1146120:intron 20.0%
!! AAAAAGATTAAATGGTTTCA+TGG + chr7_4:23241570-23241589 Msa1146120:intron 20.0%
!! AAATAAACAAGTAAATGCTA+AGG - chr7_4:23241676-23241695 None:intergenic 20.0%
!!! CAAATTCCAATTTTAAAATC+AGG + chr7_4:23242097-23242116 Msa1146120:exon 20.0%
!!! TAGTTTCCTGATTTTAAAAT+TGG - chr7_4:23242106-23242125 None:intergenic 20.0%
!! TGAACTCTTTATTATTTCAT+TGG - chr7_4:23242215-23242234 None:intergenic 20.0%
!! AAATATAATTGATTGTAGCT+TGG + chr7_4:23242262-23242281 Msa1146120:three_prime_UTR 20.0%
!!! CTTCTAAATATTTTTCATAG+GGG - chr7_4:23242345-23242364 None:intergenic 20.0%
!!! CAATTTTACAGATCAAATAA+GGG + chr7_4:23240719-23240738 Msa1146120:CDS 20.0%
!! TTATATCTTAATAAGATACC+TGG - chr7_4:23241085-23241104 None:intergenic 20.0%
!! AGGTATCTTATTAAGATATA+AGG + chr7_4:23241084-23241103 Msa1146120:CDS 20.0%
!! TAAAGATAAATTTGGTCATT+TGG - chr7_4:23241344-23241363 None:intergenic 20.0%
!! AGACATCATAAAGATAAATT+TGG - chr7_4:23241352-23241371 None:intergenic 20.0%
!! TAATTATTGTTTCCTCTATA+TGG + chr7_4:23241439-23241458 Msa1146120:intron 20.0%
!! AAAAAGATTAAATGGTTTCA+TGG + chr7_4:23241570-23241589 Msa1146120:intron 20.0%
!! AAATAAACAAGTAAATGCTA+AGG - chr7_4:23241676-23241695 None:intergenic 20.0%
!!! CAAATTCCAATTTTAAAATC+AGG + chr7_4:23242097-23242116 Msa1146120:exon 20.0%
!!! TAGTTTCCTGATTTTAAAAT+TGG - chr7_4:23242106-23242125 None:intergenic 20.0%
!! TGAACTCTTTATTATTTCAT+TGG - chr7_4:23242215-23242234 None:intergenic 20.0%
!! AAATATAATTGATTGTAGCT+TGG + chr7_4:23242262-23242281 Msa1146120:three_prime_UTR 20.0%
!!! CTTCTAAATATTTTTCATAG+GGG - chr7_4:23242345-23242364 None:intergenic 20.0%
!! GCAATTTTACAGATCAAATA+AGG + chr7_4:23240718-23240737 Msa1146120:CDS 25.0%
! AATTGAAGCTTATTTCAGAT+CGG + chr7_4:23240858-23240877 Msa1146120:CDS 25.0%
! ATGTGAAAATTAAGGGATAT+TGG - chr7_4:23241042-23241061 None:intergenic 25.0%
!! TATCCCTTAATTTTCACATA+TGG + chr7_4:23241043-23241062 Msa1146120:CDS 25.0%
! TCTCCATATGTGAAAATTAA+GGG - chr7_4:23241049-23241068 None:intergenic 25.0%
! ATAGTTAACTATCCATATAG+AGG - chr7_4:23241454-23241473 None:intergenic 25.0%
! TCGAATTAAACTATTTGTGT+AGG + chr7_4:23241476-23241495 Msa1146120:intron 25.0%
!! TTTAATCTTTTTGCAGCTTT+TGG - chr7_4:23241562-23241581 None:intergenic 25.0%
! AAAGCTGCAAAAAGATTAAA+TGG + chr7_4:23241562-23241581 Msa1146120:intron 25.0%
! AATGATACCATGAAAGATTA+GGG - chr7_4:23241607-23241626 None:intergenic 25.0%
! ATTGAAGTAAGTTCAAACAA+TGG + chr7_4:23241624-23241643 Msa1146120:intron 25.0%
!!! TATTTTATTTCATGTGACGT+TGG + chr7_4:23241709-23241728 Msa1146120:intron 25.0%
!! AATACCATCTCAATAGATTT+TGG + chr7_4:23241948-23241967 Msa1146120:intron 25.0%
! TCTTGAAAGCTATATATACA+TGG - chr7_4:23242168-23242187 None:intergenic 25.0%
! ATGTATATATAGCTTTCAAG+AGG + chr7_4:23242167-23242186 Msa1146120:three_prime_UTR 25.0%
!!! CTTAGATTTTAATTGTAGGA+AGG + chr7_4:23242374-23242393 Msa1146120:three_prime_UTR 25.0%
!! GCAATTTTACAGATCAAATA+AGG + chr7_4:23240718-23240737 Msa1146120:CDS 25.0%
! AATTGAAGCTTATTTCAGAT+CGG + chr7_4:23240858-23240877 Msa1146120:CDS 25.0%
! ATGTGAAAATTAAGGGATAT+TGG - chr7_4:23241042-23241061 None:intergenic 25.0%
!! TATCCCTTAATTTTCACATA+TGG + chr7_4:23241043-23241062 Msa1146120:CDS 25.0%
! TCTCCATATGTGAAAATTAA+GGG - chr7_4:23241049-23241068 None:intergenic 25.0%
! ATAGTTAACTATCCATATAG+AGG - chr7_4:23241454-23241473 None:intergenic 25.0%
! TCGAATTAAACTATTTGTGT+AGG + chr7_4:23241476-23241495 Msa1146120:intron 25.0%
!! TTTAATCTTTTTGCAGCTTT+TGG - chr7_4:23241562-23241581 None:intergenic 25.0%
! AAAGCTGCAAAAAGATTAAA+TGG + chr7_4:23241562-23241581 Msa1146120:intron 25.0%
! AATGATACCATGAAAGATTA+GGG - chr7_4:23241607-23241626 None:intergenic 25.0%
! ATTGAAGTAAGTTCAAACAA+TGG + chr7_4:23241624-23241643 Msa1146120:intron 25.0%
!!! TATTTTATTTCATGTGACGT+TGG + chr7_4:23241709-23241728 Msa1146120:intron 25.0%
!! AATACCATCTCAATAGATTT+TGG + chr7_4:23241948-23241967 Msa1146120:intron 25.0%
! TCTTGAAAGCTATATATACA+TGG - chr7_4:23242168-23242187 None:intergenic 25.0%
! ATGTATATATAGCTTTCAAG+AGG + chr7_4:23242167-23242186 Msa1146120:three_prime_UTR 25.0%
!!! CTTAGATTTTAATTGTAGGA+AGG + chr7_4:23242374-23242393 Msa1146120:three_prime_UTR 25.0%
!! ATCAAATAAGGGTATTGATG+AGG + chr7_4:23240730-23240749 Msa1146120:CDS 30.0%
!! TCAAATAAGGGTATTGATGA+GGG + chr7_4:23240731-23240750 Msa1146120:CDS 30.0%
ATATTGGAAACCCAAATAAC+TGG - chr7_4:23241026-23241045 None:intergenic 30.0%
CTCTCCATATGTGAAAATTA+AGG - chr7_4:23241050-23241069 None:intergenic 30.0%
! CTTAATTTTCACATATGGAG+AGG + chr7_4:23241048-23241067 Msa1146120:CDS 30.0%
GTCATTTGGAAAGTGATATT+AGG - chr7_4:23241330-23241349 None:intergenic 30.0%
ATAGCACAACTGAGTTTAAA+AGG - chr7_4:23241415-23241434 None:intergenic 30.0%
ATTTGTGTAGGAATCTCAAA+AGG + chr7_4:23241488-23241507 Msa1146120:intron 30.0%
TTTGTGTAGGAATCTCAAAA+GGG + chr7_4:23241489-23241508 Msa1146120:intron 30.0%
GTGTAATTGACATGACATAT+TGG + chr7_4:23241511-23241530 Msa1146120:intron 30.0%
CAATGATACCATGAAAGATT+AGG - chr7_4:23241608-23241627 None:intergenic 30.0%
TCAACCAAAATCTATTGAGA+TGG - chr7_4:23241955-23241974 None:intergenic 30.0%
!!! TCAATAGATTTTGGTTGAAC+AGG + chr7_4:23241957-23241976 Msa1146120:intron 30.0%
!! ATCAAATAAGGGTATTGATG+AGG + chr7_4:23240730-23240749 Msa1146120:CDS 30.0%
!! TCAAATAAGGGTATTGATGA+GGG + chr7_4:23240731-23240750 Msa1146120:CDS 30.0%
ATATTGGAAACCCAAATAAC+TGG - chr7_4:23241026-23241045 None:intergenic 30.0%
CTCTCCATATGTGAAAATTA+AGG - chr7_4:23241050-23241069 None:intergenic 30.0%
! CTTAATTTTCACATATGGAG+AGG + chr7_4:23241048-23241067 Msa1146120:CDS 30.0%
GTCATTTGGAAAGTGATATT+AGG - chr7_4:23241330-23241349 None:intergenic 30.0%
ATAGCACAACTGAGTTTAAA+AGG - chr7_4:23241415-23241434 None:intergenic 30.0%
ATTTGTGTAGGAATCTCAAA+AGG + chr7_4:23241488-23241507 Msa1146120:intron 30.0%
TTTGTGTAGGAATCTCAAAA+GGG + chr7_4:23241489-23241508 Msa1146120:intron 30.0%
GTGTAATTGACATGACATAT+TGG + chr7_4:23241511-23241530 Msa1146120:intron 30.0%
CAATGATACCATGAAAGATT+AGG - chr7_4:23241608-23241627 None:intergenic 30.0%
TCAACCAAAATCTATTGAGA+TGG - chr7_4:23241955-23241974 None:intergenic 30.0%
!!! TCAATAGATTTTGGTTGAAC+AGG + chr7_4:23241957-23241976 Msa1146120:intron 30.0%
ATTTCAGATCGGAACAATGA+TGG + chr7_4:23240869-23240888 Msa1146120:CDS 35.0%
CGTAGAGATATTATCCTTCA+CGG + chr7_4:23240962-23240981 Msa1146120:CDS 35.0%
! ATTTTCACATATGGAGAGGA+TGG + chr7_4:23241052-23241071 Msa1146120:CDS 35.0%
!! CTATGACTTTGATTTGTTCC+AGG + chr7_4:23241188-23241207 Msa1146120:intron 35.0%
! TGGTTGGATTTGGAATCTAT+GGG - chr7_4:23241542-23241561 None:intergenic 35.0%
! TTGGTTGGATTTGGAATCTA+TGG - chr7_4:23241543-23241562 None:intergenic 35.0%
! ATCTTTTTGCAGCTTTTGGT+TGG - chr7_4:23241558-23241577 None:intergenic 35.0%
TTACCTTAATGTGGTTGTTG+CGG + chr7_4:23241777-23241796 Msa1146120:intron 35.0%
AACAGGCTTCATAAAAGGTT+GGG + chr7_4:23241974-23241993 Msa1146120:intron 35.0%
ATTTCAGATCGGAACAATGA+TGG + chr7_4:23240869-23240888 Msa1146120:CDS 35.0%
CGTAGAGATATTATCCTTCA+CGG + chr7_4:23240962-23240981 Msa1146120:CDS 35.0%
! ATTTTCACATATGGAGAGGA+TGG + chr7_4:23241052-23241071 Msa1146120:CDS 35.0%
!! CTATGACTTTGATTTGTTCC+AGG + chr7_4:23241188-23241207 Msa1146120:intron 35.0%
! TGGTTGGATTTGGAATCTAT+GGG - chr7_4:23241542-23241561 None:intergenic 35.0%
! TTGGTTGGATTTGGAATCTA+TGG - chr7_4:23241543-23241562 None:intergenic 35.0%
! ATCTTTTTGCAGCTTTTGGT+TGG - chr7_4:23241558-23241577 None:intergenic 35.0%
TTACCTTAATGTGGTTGTTG+CGG + chr7_4:23241777-23241796 Msa1146120:intron 35.0%
AACAGGCTTCATAAAAGGTT+GGG + chr7_4:23241974-23241993 Msa1146120:intron 35.0%
GTCTATATCTCCGTCAATCA+GGG - chr7_4:23240933-23240952 None:intergenic 40.0%
AGTCTATATCTCCGTCAATC+AGG - chr7_4:23240934-23240953 None:intergenic 40.0%
AAGGATAATATCTCTACGCG+AGG - chr7_4:23240960-23240979 None:intergenic 40.0%
ATTATCCTTCACGGACAATC+AGG + chr7_4:23240971-23240990 Msa1146120:CDS 40.0%
TGAGTTCCATGCCAGTTATT+TGG + chr7_4:23241012-23241031 Msa1146120:CDS 40.0%
GAGTTCCATGCCAGTTATTT+GGG + chr7_4:23241013-23241032 Msa1146120:CDS 40.0%
GCACAACCGTTTGACTATAA+AGG + chr7_4:23241126-23241145 Msa1146120:CDS 40.0%
ACCGTTTGACTATAAAGGCA+TGG + chr7_4:23241131-23241150 Msa1146120:CDS 40.0%
GCCATGCCTTTATAGTCAAA+CGG - chr7_4:23241135-23241154 None:intergenic 40.0%
ACTAATGTCCAGACTATGTG+AGG + chr7_4:23241165-23241184 Msa1146120:CDS 40.0%
!! TTGCAGCTTTTGGTTGGATT+TGG - chr7_4:23241552-23241571 None:intergenic 40.0%
ACCTCATCCCTAATCTTTCA+TGG + chr7_4:23241597-23241616 Msa1146120:intron 40.0%
ACCATGAAAGATTAGGGATG+AGG - chr7_4:23241601-23241620 None:intergenic 40.0%
TATTTCATGTGACGTTGGTG+TGG + chr7_4:23241714-23241733 Msa1146120:intron 40.0%
GCTTTCAACAGTCAACTCAT+GGG - chr7_4:23241816-23241835 None:intergenic 40.0%
AGCTTTCAACAGTCAACTCA+TGG - chr7_4:23241817-23241836 None:intergenic 40.0%
GAGTTGACTGTTGAAAGCTA+TGG + chr7_4:23241818-23241837 Msa1146120:intron 40.0%
GACTGTTGAAAGCTATGGAT+TGG + chr7_4:23241823-23241842 Msa1146120:intron 40.0%
GGTTGAACAGGCTTCATAAA+AGG + chr7_4:23241969-23241988 Msa1146120:intron 40.0%
GAACAGGCTTCATAAAAGGT+TGG + chr7_4:23241973-23241992 Msa1146120:intron 40.0%
! CTTTCAAGAGGAGCGTATAA+GGG + chr7_4:23242179-23242198 Msa1146120:three_prime_UTR 40.0%
! TTTCAAGAGGAGCGTATAAG+GGG + chr7_4:23242180-23242199 Msa1146120:three_prime_UTR 40.0%
!! TACGCACTTGTAGCTTTATC+TGG + chr7_4:23242311-23242330 Msa1146120:three_prime_UTR 40.0%
!! CACTTGTAGCTTTATCTGGT+GGG + chr7_4:23242315-23242334 Msa1146120:three_prime_UTR 40.0%
GTCTATATCTCCGTCAATCA+GGG - chr7_4:23240933-23240952 None:intergenic 40.0%
AGTCTATATCTCCGTCAATC+AGG - chr7_4:23240934-23240953 None:intergenic 40.0%
AAGGATAATATCTCTACGCG+AGG - chr7_4:23240960-23240979 None:intergenic 40.0%
ATTATCCTTCACGGACAATC+AGG + chr7_4:23240971-23240990 Msa1146120:CDS 40.0%
TGAGTTCCATGCCAGTTATT+TGG + chr7_4:23241012-23241031 Msa1146120:CDS 40.0%
GAGTTCCATGCCAGTTATTT+GGG + chr7_4:23241013-23241032 Msa1146120:CDS 40.0%
GCACAACCGTTTGACTATAA+AGG + chr7_4:23241126-23241145 Msa1146120:CDS 40.0%
ACCGTTTGACTATAAAGGCA+TGG + chr7_4:23241131-23241150 Msa1146120:CDS 40.0%
GCCATGCCTTTATAGTCAAA+CGG - chr7_4:23241135-23241154 None:intergenic 40.0%
ACTAATGTCCAGACTATGTG+AGG + chr7_4:23241165-23241184 Msa1146120:CDS 40.0%
!! TTGCAGCTTTTGGTTGGATT+TGG - chr7_4:23241552-23241571 None:intergenic 40.0%
ACCTCATCCCTAATCTTTCA+TGG + chr7_4:23241597-23241616 Msa1146120:intron 40.0%
ACCATGAAAGATTAGGGATG+AGG - chr7_4:23241601-23241620 None:intergenic 40.0%
TATTTCATGTGACGTTGGTG+TGG + chr7_4:23241714-23241733 Msa1146120:intron 40.0%
GCTTTCAACAGTCAACTCAT+GGG - chr7_4:23241816-23241835 None:intergenic 40.0%
AGCTTTCAACAGTCAACTCA+TGG - chr7_4:23241817-23241836 None:intergenic 40.0%
GAGTTGACTGTTGAAAGCTA+TGG + chr7_4:23241818-23241837 Msa1146120:intron 40.0%
GACTGTTGAAAGCTATGGAT+TGG + chr7_4:23241823-23241842 Msa1146120:intron 40.0%
GGTTGAACAGGCTTCATAAA+AGG + chr7_4:23241969-23241988 Msa1146120:intron 40.0%
GAACAGGCTTCATAAAAGGT+TGG + chr7_4:23241973-23241992 Msa1146120:intron 40.0%
! CTTTCAAGAGGAGCGTATAA+GGG + chr7_4:23242179-23242198 Msa1146120:three_prime_UTR 40.0%
! TTTCAAGAGGAGCGTATAAG+GGG + chr7_4:23242180-23242199 Msa1146120:three_prime_UTR 40.0%
!! TACGCACTTGTAGCTTTATC+TGG + chr7_4:23242311-23242330 Msa1146120:three_prime_UTR 40.0%
!! CACTTGTAGCTTTATCTGGT+GGG + chr7_4:23242315-23242334 Msa1146120:three_prime_UTR 40.0%
TGCAAACAATGTGCATGCAC+AGG + chr7_4:23240784-23240803 Msa1146120:CDS 45.0%
GGAAACCCAAATAACTGGCA+TGG - chr7_4:23241021-23241040 None:intergenic 45.0%
AGTCATAGCCTCACATAGTC+TGG - chr7_4:23241176-23241195 None:intergenic 45.0%
CAACCGCAACAACCACATTA+AGG - chr7_4:23241783-23241802 None:intergenic 45.0%
!! GCTATGGATTGGAGACATCT+TGG + chr7_4:23241834-23241853 Msa1146120:intron 45.0%
! AGGCATGGAGGATTTAGCTT+CGG - chr7_4:23242020-23242039 None:intergenic 45.0%
GATTCTTAGTCTGCAGTTCC+AGG - chr7_4:23242060-23242079 None:intergenic 45.0%
! GCTTTCAAGAGGAGCGTATA+AGG + chr7_4:23242178-23242197 Msa1146120:three_prime_UTR 45.0%
! TTCAAGAGGAGCGTATAAGG+GGG + chr7_4:23242181-23242200 Msa1146120:three_prime_UTR 45.0%
!! GCACTTGTAGCTTTATCTGG+TGG + chr7_4:23242314-23242333 Msa1146120:three_prime_UTR 45.0%
TGCAAACAATGTGCATGCAC+AGG + chr7_4:23240784-23240803 Msa1146120:CDS 45.0%
GGAAACCCAAATAACTGGCA+TGG - chr7_4:23241021-23241040 None:intergenic 45.0%
AGTCATAGCCTCACATAGTC+TGG - chr7_4:23241176-23241195 None:intergenic 45.0%
CAACCGCAACAACCACATTA+AGG - chr7_4:23241783-23241802 None:intergenic 45.0%
!! GCTATGGATTGGAGACATCT+TGG + chr7_4:23241834-23241853 Msa1146120:intron 45.0%
! AGGCATGGAGGATTTAGCTT+CGG - chr7_4:23242020-23242039 None:intergenic 45.0%
GATTCTTAGTCTGCAGTTCC+AGG - chr7_4:23242060-23242079 None:intergenic 45.0%
! GCTTTCAAGAGGAGCGTATA+AGG + chr7_4:23242178-23242197 Msa1146120:three_prime_UTR 45.0%
! TTCAAGAGGAGCGTATAAGG+GGG + chr7_4:23242181-23242200 Msa1146120:three_prime_UTR 45.0%
!! GCACTTGTAGCTTTATCTGG+TGG + chr7_4:23242314-23242333 Msa1146120:three_prime_UTR 45.0%
GCATGCACAGGCTTTCAGAA+TGG + chr7_4:23240796-23240815 Msa1146120:CDS 50.0%
GCAGCTACTTCTGACACAGT+TGG - chr7_4:23240911-23240930 None:intergenic 50.0%
GAAGTAGCTGCCCTGATTGA+CGG + chr7_4:23240920-23240939 Msa1146120:CDS 50.0%
GTTCACCTGATTGTCCGTGA+AGG - chr7_4:23240979-23240998 None:intergenic 50.0%
! GGCATGGAGGATTTAGCTTC+GGG - chr7_4:23242019-23242038 None:intergenic 50.0%
AAATCCTCCATGCCTCCACT+TGG + chr7_4:23242025-23242044 Msa1146120:CDS 50.0%
TAGTCTGCAGTTCCAGGTAG+AGG - chr7_4:23242054-23242073 None:intergenic 50.0%
GCATGCACAGGCTTTCAGAA+TGG + chr7_4:23240796-23240815 Msa1146120:CDS 50.0%
GCAGCTACTTCTGACACAGT+TGG - chr7_4:23240911-23240930 None:intergenic 50.0%
GAAGTAGCTGCCCTGATTGA+CGG + chr7_4:23240920-23240939 Msa1146120:CDS 50.0%
GTTCACCTGATTGTCCGTGA+AGG - chr7_4:23240979-23240998 None:intergenic 50.0%
! GGCATGGAGGATTTAGCTTC+GGG - chr7_4:23242019-23242038 None:intergenic 50.0%
AAATCCTCCATGCCTCCACT+TGG + chr7_4:23242025-23242044 Msa1146120:CDS 50.0%
TAGTCTGCAGTTCCAGGTAG+AGG - chr7_4:23242054-23242073 None:intergenic 50.0%
GGAGAGGATGGATATCGTCC+AGG + chr7_4:23241064-23241083 Msa1146120:CDS 55.0%
GTGGTTGTTGCGGTTGCTTC+AGG + chr7_4:23241787-23241806 Msa1146120:intron 55.0%
GTTGTTGCGGTTGCTTCAGG+AGG + chr7_4:23241790-23241809 Msa1146120:intron 55.0%
!! AGGTAGAGGCATCCAAGTGG+AGG - chr7_4:23242040-23242059 None:intergenic 55.0%
TCCACTTGGATGCCTCTACC+TGG + chr7_4:23242039-23242058 Msa1146120:CDS 55.0%
!! TCCAGGTAGAGGCATCCAAG+TGG - chr7_4:23242043-23242062 None:intergenic 55.0%
GGAGAGGATGGATATCGTCC+AGG + chr7_4:23241064-23241083 Msa1146120:CDS 55.0%
GTGGTTGTTGCGGTTGCTTC+AGG + chr7_4:23241787-23241806 Msa1146120:intron 55.0%
GTTGTTGCGGTTGCTTCAGG+AGG + chr7_4:23241790-23241809 Msa1146120:intron 55.0%
!! AGGTAGAGGCATCCAAGTGG+AGG - chr7_4:23242040-23242059 None:intergenic 55.0%
TCCACTTGGATGCCTCTACC+TGG + chr7_4:23242039-23242058 Msa1146120:CDS 55.0%
!! TCCAGGTAGAGGCATCCAAG+TGG - chr7_4:23242043-23242062 None:intergenic 55.0%
! GCATCCAAGTGGAGGCATGG+AGG - chr7_4:23242032-23242051 None:intergenic 60.0%
! GAGGCATCCAAGTGGAGGCA+TGG - chr7_4:23242035-23242054 None:intergenic 60.0%
! GCATCCAAGTGGAGGCATGG+AGG - chr7_4:23242032-23242051 None:intergenic 60.0%
! GAGGCATCCAAGTGGAGGCA+TGG - chr7_4:23242035-23242054 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 23240716 23242439 23240716 ID=Msa1146120;Name=Msa1146120
chr7_4 mRNA 23240716 23242439 23240716 ID=Msa1146120-mRNA-1;Parent=Msa1146120;Name=Msa1146120-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|0|0.5|1|0|0.5|2|327|206
chr7_4 exon 23240716 23241202 23240716 ID=Msa1146120-mRNA-1:exon:5847;Parent=Msa1146120-mRNA-1
chr7_4 exon 23241979 23242439 23241979 ID=Msa1146120-mRNA-1:exon:5848;Parent=Msa1146120-mRNA-1
chr7_4 CDS 23240716 23241202 23240716 ID=Msa1146120-mRNA-1:cds;Parent=Msa1146120-mRNA-1
chr7_4 CDS 23241979 23242112 23241979 ID=Msa1146120-mRNA-1:cds;Parent=Msa1146120-mRNA-1
chr7_4 three_prime_UTR 23242113 23242439 23242113 ID=Msa1146120-mRNA-1:three_prime_utr;Parent=Msa1146120-mRNA-1
Gene Sequence

>Msa1146120

ATGCAATTTTACAGATCAAATAAGGGTATTGATGAGGGCATTGTAAAAGACTTGAAAGATATGATTGATGCAAACAATGTGCATGCACAGGCTTTCAGAATGGCACGAGATATGTTACAAGATGTTCAATTTCAAGATGTAAAATTGAAGCTTATTTCAGATCGGAACAATGATGGAAGAATATACAACAAACCAACTGTGTCAGAAGTAGCTGCCCTGATTGACGGAGATATAGACTCAGCCTCGCGTAGAGATATTATCCTTCACGGACAATCAGGTGAACTTCAAAGAATTGATGAGTTCCATGCCAGTTATTTGGGTTTCCAATATCCCTTAATTTTCACATATGGAGAGGATGGATATCGTCCAGGTATCTTATTAAGATATAAGGATGATACAGTCATTAATAAGCACAACCGTTTGACTATAAAGGCATGGCTATCATTTCGACTAATGTCCAGACTATGTGAGGCTATGACTTTGATTTGCTTCATAAAAGGTTGGGACTTCTCAAATTCTGAGAACCCGAAGCTAAATCCTCCATGCCTCCACTTGGATGCCTCTACCTGGAACTGCAGACTAAGAATCAAAAGAGTATTACATTTCAAATTCCAATTTTAA
Protein sequence

>Msa1146120

MQFYRSNKGIDEGIVKDLKDMIDANNVHAQAFRMARDMLQDVQFQDVKLKLISDRNNDGRIYNKPTVSEVAALIDGDIDSASRRDIILHGQSGELQRIDEFHASYLGFQYPLIFTYGEDGYRPGILLRYKDDTVINKHNRLTIKAWLSFRLMSRLCEAMTLICFIKGWDFSNSENPKLNPPCLHLDASTWNCRLRIKRVLHFKFQF*