AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1181800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1181800 MtrunA17_Chr8g0336781 71.429 175 17 1 1 142 36 210 2.01e-84 253
Msa1181800 MtrunA17_Chr8g0336791 62.857 175 32 1 1 142 1 175 3.02e-77 228
Msa1181800 MtrunA17_Chr8g0336831 62.286 175 33 1 1 142 1 175 1.79e-75 225
Msa1181800 MtrunA17_Chr8g0336861 64.571 175 29 1 1 142 1 175 1.81e-74 226
Msa1181800 MtrunA17_Chr8g0336851 65.714 175 26 2 1 142 1 174 2.34e-74 226
Msa1181800 MtrunA17_Chr8g0348081 64.571 175 28 2 1 142 1 174 4.29e-73 223
Msa1181800 MtrunA17_Chr8g0336631 65.341 176 26 3 1 142 1 175 1.21e-72 221
Msa1181800 MtrunA17_Chr8g0336761 65.714 175 26 2 1 142 1 174 3.26e-72 220
Msa1181800 MtrunA17_Chr4g0066121 56.250 176 42 3 1 142 1 175 4.23e-60 189
Msa1181800 MtrunA17_Chr2g0311761 53.179 173 44 4 5 142 11 181 9.69e-51 166
Msa1181800 MtrunA17_Chr2g0311831 51.429 175 44 5 5 142 11 181 1.31e-47 157
Msa1181800 MtrunA17_Chr2g0311921 50.920 163 44 3 15 142 6 167 2.67e-46 154
Msa1181800 MtrunA17_Chr2g0311861 50.633 158 61 5 1 142 1 157 3.58e-40 137
Msa1181800 MtrunA17_Chr8g0336751 44.186 172 59 4 6 142 38 207 7.77e-39 135
Msa1181800 MtrunA17_Chr1g0168001 45.238 168 50 5 15 142 7 172 9.32e-38 127
Msa1181800 MtrunA17_Chr1g0167951 42.857 161 57 4 15 142 7 165 9.15e-37 129
Msa1181800 MtrunA17_Chr1g0167911 44.444 162 54 4 15 142 7 166 1.03e-36 129
Msa1181800 MtrunA17_Chr1g0167961 44.172 163 54 5 15 142 22 182 2.45e-36 128
Msa1181800 MtrunA17_Chr8g0354261 43.976 166 51 4 16 142 21 183 5.16e-36 127
Msa1181800 MtrunA17_Chr8g0354241 42.771 166 53 4 16 142 21 183 3.71e-34 122
Msa1181800 MtrunA17_Chr6g0472861 38.462 169 62 4 15 142 15 182 7.14e-29 108
Msa1181800 MtrunA17_Chr7g0235951 37.500 160 65 4 17 142 22 180 8.04e-29 108
Msa1181800 MtrunA17_Chr7g0236011 36.095 169 73 3 17 151 22 189 1.02e-28 108
Msa1181800 MtrunA17_Chr7g0247991 38.272 162 61 4 17 142 22 180 1.31e-28 107
Msa1181800 MtrunA17_Chr1g0187811 38.596 171 61 5 15 142 132 301 1.33e-28 109
Msa1181800 MtrunA17_Chr4g0055591 37.580 157 66 3 17 142 14 169 2.14e-28 107
Msa1181800 MtrunA17_Chr7g0225881 35.955 178 77 3 1 142 1 177 4.00e-28 106
Msa1181800 MtrunA17_Chr8g0364471 37.500 160 65 4 17 142 22 180 6.59e-28 106
Msa1181800 MtrunA17_Chr1g0187821 37.349 166 61 5 17 142 18 180 1.59e-27 105
Msa1181800 MtrunA17_Chr8g0335411 35.000 160 69 3 17 142 22 180 1.29e-26 102
Msa1181800 MtrunA17_Chr4g0011311 35.625 160 68 4 17 142 22 180 1.62e-25 98.6
Msa1181800 MtrunA17_Chr1g0187831 36.310 168 66 5 15 142 17 183 3.21e-25 99.0
Msa1181800 MtrunA17_Chr8g0362741 38.028 142 67 4 17 138 22 162 3.69e-25 97.4
Msa1181800 MtrunA17_Chr8g0364161 35.897 156 65 4 17 138 22 176 5.58e-25 94.7
Msa1181800 MtrunA17_Chr2g0311711 34.161 161 69 2 17 141 20 179 1.36e-24 97.1
Msa1181800 MtrunA17_Chr7g0235981 37.984 129 75 4 17 142 8 134 1.37e-23 94.0
Msa1181800 MtrunA17_Chr2g0311701 33.540 161 70 4 17 141 22 181 2.13e-23 94.0
Msa1181800 MtrunA17_Chr1g0184241 34.375 160 71 3 16 142 22 180 5.50e-23 92.8
Msa1181800 MtrunA17_Chr1g0161081 31.677 161 76 2 15 142 24 183 6.41e-22 90.1
Msa1181800 MtrunA17_Chr2g0311681 32.768 177 81 4 1 141 1 175 1.70e-21 88.6
Msa1181800 MtrunA17_Chr2g0311751 44.211 95 52 1 49 143 48 141 2.34e-21 84.7
Msa1181800 MtrunA17_Chr2g0311691 33.898 177 79 5 1 141 1 175 4.27e-21 87.8
Msa1181800 MtrunA17_Chr7g0266651 30.864 162 77 3 15 142 11 171 1.47e-20 86.3
Msa1181800 MtrunA17_Chr2g0311741 46.809 94 49 1 49 142 48 140 2.54e-20 85.1
Msa1181800 MtrunA17_Chr1g0161111 30.435 161 78 2 15 142 83 242 2.77e-20 85.9
Msa1181800 MtrunA17_Chr1g0161041 31.677 161 76 2 15 142 47 206 1.10e-19 84.0
Msa1181800 MtrunA17_Chr7g0235991 38.043 92 56 1 51 142 44 134 2.91e-17 74.7
Msa1181800 MtrunA17_Chr4g0066991 31.875 160 54 4 17 142 22 160 4.02e-17 76.6
Msa1181800 MtrunA17_Chr1g0167931 39.394 99 26 2 15 79 7 105 5.82e-13 64.7
Msa1181800 MtrunA17_Chr1g0161101 35.714 112 64 3 16 122 100 208 3.86e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1181800 AT5G59540.2 46.341 164 49 5 17 142 14 176 3.42e-39 134
Msa1181800 AT5G59540.1 46.341 164 49 5 17 142 14 176 1.27e-38 134
Msa1181800 AT1G04350.1 44.099 161 55 4 17 143 11 170 7.58e-37 129
Msa1181800 AT1G06620.2 41.772 158 56 2 19 141 18 174 4.81e-36 125
Msa1181800 AT1G06650.1 41.818 165 56 3 17 142 15 178 1.34e-35 125
Msa1181800 AT1G06620.1 41.772 158 56 2 19 141 18 174 2.65e-35 125
Msa1181800 AT1G06650.2 41.818 165 56 3 17 142 15 178 3.59e-35 125
Msa1181800 AT1G06640.3 40.606 165 58 3 17 142 15 178 3.68e-35 124
Msa1181800 AT1G06640.2 40.606 165 58 3 17 142 15 178 5.51e-35 124
Msa1181800 AT1G06640.1 40.606 165 58 3 17 142 15 178 1.50e-34 124
Msa1181800 AT2G30840.2 39.877 163 59 3 17 141 10 171 1.51e-34 122
Msa1181800 AT5G43440.2 39.394 165 63 2 15 142 11 175 4.35e-34 121
Msa1181800 AT2G30840.1 39.877 163 59 3 17 141 10 171 4.47e-34 122
Msa1181800 AT2G30840.3 39.877 163 59 3 17 141 10 171 5.19e-34 122
Msa1181800 AT5G43440.1 39.394 165 63 2 15 142 11 175 2.35e-33 120
Msa1181800 AT2G30830.1 39.375 160 62 3 17 142 10 168 7.16e-33 119
Msa1181800 AT5G59530.1 43.636 165 51 7 17 142 13 174 8.28e-32 116
Msa1181800 AT1G06645.1 39.735 151 52 1 17 128 28 178 5.14e-31 114
Msa1181800 AT5G43450.2 38.650 163 64 3 15 142 11 172 6.24e-31 112
Msa1181800 AT5G43450.1 38.650 163 64 3 15 142 11 172 1.89e-30 113
Msa1181800 AT1G03410.2 38.037 163 62 4 17 141 9 170 1.49e-29 110
Msa1181800 AT1G03410.1 38.037 163 62 4 17 141 46 207 1.89e-29 110
Msa1181800 AT2G25450.1 37.107 159 65 3 17 141 10 167 2.82e-29 109
Msa1181800 AT1G04380.2 50.526 95 46 1 48 142 62 155 7.44e-29 107
Msa1181800 AT1G04380.1 50.526 95 46 1 48 142 62 155 3.25e-28 107
Msa1181800 AT1G03400.1 37.908 153 66 2 17 141 9 160 1.09e-27 105
Msa1181800 AT1G03400.2 37.908 153 66 2 17 141 9 160 1.59e-27 105
Msa1181800 AT3G61400.2 34.132 167 69 2 15 141 12 177 1.34e-26 101
Msa1181800 AT3G61400.1 34.132 167 69 2 15 141 12 177 3.47e-26 101

Find 30 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACAAAAGCTGGTGTAAA+AGG 0.285364 8_1:+16335065 Msa1181800:CDS
TCTTCTGGTTTGAGAGTATC+AGG 0.295106 8_1:-16335482 None:intergenic
CACAGCACAGGAAACTCTTC+TGG 0.345221 8_1:-16335497 None:intergenic
TTGGCTTTCTCATGTTTGTC+AGG 0.408748 8_1:-16335137 None:intergenic
ATAGCTCACCAGCACTCAAT+TGG 0.417970 8_1:+16335429 Msa1181800:CDS
AATAACATGGCTTGTGTTGT+TGG 0.428788 8_1:-16335156 None:intergenic
CATGTTTGTCAGGTTGATGA+TGG 0.437515 8_1:-16335127 None:intergenic
AAGAGTTTCCTGTGCTGTGC+AGG 0.457821 8_1:+16335501 Msa1181800:CDS
GTTCTTTCAATATAGAATCA+AGG 0.486469 8_1:-16335028 None:intergenic
TTCAATATAGAATCAAGGTT+AGG 0.492206 8_1:-16335023 None:intergenic
ATCTCCTCAAGAACACTCAA+AGG 0.510634 8_1:-16335299 None:intergenic
GAGTGTTCTTGAGGAGATGA+AGG 0.513387 8_1:+16335304 Msa1181800:CDS
GGTGTAAAAGGACTTGTTGA+TGG 0.518526 8_1:+16335077 Msa1181800:CDS
TATAGAATCAAGGTTAGGCT+TGG 0.520854 8_1:-16335018 None:intergenic
GCTCACCAGCACTCAATTGG+AGG 0.527842 8_1:+16335432 Msa1181800:CDS
CTATCCCTCCAATTGAGTGC+TGG 0.534831 8_1:-16335437 None:intergenic
TCTCCTCAAGAACACTCAAA+GGG 0.535297 8_1:-16335298 None:intergenic
GGTTGATGATGGAATAAACT+TGG 0.541944 8_1:-16335116 None:intergenic
AGATGAAGGATGGTGTGAAG+AGG 0.547649 8_1:+16335318 Msa1181800:CDS
AATCCCTTTGAGTGTTCTTG+AGG 0.553310 8_1:+16335295 Msa1181800:CDS
TGTGAAGAGGTTTCATGAGA+TGG 0.568486 8_1:+16335331 Msa1181800:CDS
GAATTTGATGAAACAAAAGC+TGG 0.578863 8_1:+16335056 Msa1181800:CDS
AAAATTACTATTATAAACAA+AGG 0.603110 8_1:-16335399 None:intergenic
GTTAGGCTTGGTTGTACCCG+CGG 0.609084 8_1:-16335006 None:intergenic
TATACATACCTGCACAGCAC+AGG 0.626589 8_1:-16335509 None:intergenic
CTCACCAGCACTCAATTGGA+GGG 0.626812 8_1:+16335433 Msa1181800:CDS
GTTCTTGAGGAGATGAAGGA+TGG 0.628456 8_1:+16335308 Msa1181800:CDS
TCTGGTTTGAGAGTATCAGG+TGG 0.638557 8_1:-16335479 None:intergenic
GGTCTATGACAGGAATAACA+TGG 0.670694 8_1:-16335169 None:intergenic
GTAAAAGGACTTGTTGATGG+AGG 0.724997 8_1:+16335080 Msa1181800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTACTATTATAAACAA+AGG - chr8_1:16335402-16335421 None:intergenic 10.0%
! TTCAATATAGAATCAAGGTT+AGG - chr8_1:16335026-16335045 None:intergenic 25.0%
! GTTCTTTCAATATAGAATCA+AGG - chr8_1:16335031-16335050 None:intergenic 25.0%
! CTTTATTGTCAATCTTATCA+AGG - chr8_1:16335193-16335212 None:intergenic 25.0%
!!! TTTTTTCAGGTTGTTAATCA+TGG + chr8_1:16335272-16335291 Msa1181800:intron 25.0%
! TTTATTTGCTCCTTGAAATT+TGG + chr8_1:16335547-16335566 Msa1181800:intron 25.0%
! ATGATCATAACCAAATTTCA+AGG - chr8_1:16335560-16335579 None:intergenic 25.0%
GAATTTGATGAAACAAAAGC+TGG + chr8_1:16335056-16335075 Msa1181800:CDS 30.0%
AAAGATCCAAGTATTCATCA+AGG + chr8_1:16335209-16335228 Msa1181800:intron 30.0%
AAGATCCAAGTATTCATCAA+GGG + chr8_1:16335210-16335229 Msa1181800:intron 30.0%
!!! TGAAACTTGAGGTTTTTTTC+AGG + chr8_1:16335259-16335278 Msa1181800:intron 30.0%
GTAGCACTAAAATTTCAGAT+TGG + chr8_1:16335582-16335601 Msa1181800:intron 30.0%
TATAGAATCAAGGTTAGGCT+TGG - chr8_1:16335021-16335040 None:intergenic 35.0%
GAAACAAAAGCTGGTGTAAA+AGG + chr8_1:16335065-16335084 Msa1181800:CDS 35.0%
!! GGTTGATGATGGAATAAACT+TGG - chr8_1:16335119-16335138 None:intergenic 35.0%
! AATAACATGGCTTGTGTTGT+TGG - chr8_1:16335159-16335178 None:intergenic 35.0%
ATCTTATCAAGGTCTATGAC+AGG - chr8_1:16335182-16335201 None:intergenic 35.0%
ACAATCCCTTGATGAATACT+TGG - chr8_1:16335218-16335237 None:intergenic 35.0%
AGGGATTGTTAGCAAAATCA+AGG + chr8_1:16335229-16335248 Msa1181800:intron 35.0%
!! GGTGTAAAAGGACTTGTTGA+TGG + chr8_1:16335077-16335096 Msa1181800:CDS 40.0%
!! GTAAAAGGACTTGTTGATGG+AGG + chr8_1:16335080-16335099 Msa1181800:CDS 40.0%
!! CATGTTTGTCAGGTTGATGA+TGG - chr8_1:16335130-16335149 None:intergenic 40.0%
! TTGGCTTTCTCATGTTTGTC+AGG - chr8_1:16335140-16335159 None:intergenic 40.0%
GGTCTATGACAGGAATAACA+TGG - chr8_1:16335172-16335191 None:intergenic 40.0%
! AAGGAAGCATGTGAAACTTG+AGG + chr8_1:16335248-16335267 Msa1181800:intron 40.0%
AATCCCTTTGAGTGTTCTTG+AGG + chr8_1:16335295-16335314 Msa1181800:CDS 40.0%
TCTCCTCAAGAACACTCAAA+GGG - chr8_1:16335301-16335320 None:intergenic 40.0%
ATCTCCTCAAGAACACTCAA+AGG - chr8_1:16335302-16335321 None:intergenic 40.0%
TGTGAAGAGGTTTCATGAGA+TGG + chr8_1:16335331-16335350 Msa1181800:CDS 40.0%
!! TCTTCTGGTTTGAGAGTATC+AGG - chr8_1:16335485-16335504 None:intergenic 40.0%
GAGTGTTCTTGAGGAGATGA+AGG + chr8_1:16335304-16335323 Msa1181800:CDS 45.0%
GTTCTTGAGGAGATGAAGGA+TGG + chr8_1:16335308-16335327 Msa1181800:CDS 45.0%
AGATGAAGGATGGTGTGAAG+AGG + chr8_1:16335318-16335337 Msa1181800:CDS 45.0%
ATAGCTCACCAGCACTCAAT+TGG + chr8_1:16335429-16335448 Msa1181800:CDS 45.0%
!! TCTGGTTTGAGAGTATCAGG+TGG - chr8_1:16335482-16335501 None:intergenic 45.0%
TATACATACCTGCACAGCAC+AGG - chr8_1:16335512-16335531 None:intergenic 45.0%
CTCACCAGCACTCAATTGGA+GGG + chr8_1:16335433-16335452 Msa1181800:CDS 50.0%
CTATCCCTCCAATTGAGTGC+TGG - chr8_1:16335440-16335459 None:intergenic 50.0%
CACAGCACAGGAAACTCTTC+TGG - chr8_1:16335500-16335519 None:intergenic 50.0%
AAGAGTTTCCTGTGCTGTGC+AGG + chr8_1:16335501-16335520 Msa1181800:CDS 50.0%
! GTTAGGCTTGGTTGTACCCG+CGG - chr8_1:16335009-16335028 None:intergenic 55.0%
GCTCACCAGCACTCAATTGG+AGG + chr8_1:16335432-16335451 Msa1181800:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 16334999 16335630 16334999 ID=Msa1181800;Name=Msa1181800
chr8_1 mRNA 16334999 16335630 16334999 ID=Msa1181800-mRNA-1;Parent=Msa1181800;Name=Msa1181800-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|0.66|1|1|3|0|151
chr8_1 exon 16334999 16335181 16334999 ID=Msa1181800-mRNA-1:exon:6134;Parent=Msa1181800-mRNA-1
chr8_1 exon 16335281 16335522 16335281 ID=Msa1181800-mRNA-1:exon:6135;Parent=Msa1181800-mRNA-1
chr8_1 exon 16335600 16335630 16335600 ID=Msa1181800-mRNA-1:exon:6136;Parent=Msa1181800-mRNA-1
chr8_1 CDS 16334999 16335181 16334999 ID=Msa1181800-mRNA-1:cds;Parent=Msa1181800-mRNA-1
chr8_1 CDS 16335281 16335522 16335281 ID=Msa1181800-mRNA-1:cds;Parent=Msa1181800-mRNA-1
chr8_1 CDS 16335600 16335630 16335600 ID=Msa1181800-mRNA-1:cds;Parent=Msa1181800-mRNA-1
Gene Sequence

>Msa1181800

ATGGGAACCGCGGGTACAACCAAGCCTAACCTTGATTCTATATTGAAAGAACGCAAAGAATTTGATGAAACAAAAGCTGGTGTAAAAGGACTTGTTGATGGAGGACTTAAGAAAATACCAAGTTTATTCCATCATCAACCTGACAAACATGAGAAAGCCAACAACACAAGCCATGTTATTCCTGTTGTTAATCATGGAATCCCTTTGAGTGTTCTTGAGGAGATGAAGGATGGTGTGAAGAGGTTTCATGAGATGGATACAGATGCTAAAAAAGAATTGTATACTAGAGATAGCAAGAGATCCTTTGTTTATAATAGTAATTTTGATCTTTATAGCTCACCAGCACTCAATTGGAGGGATAGTTTTATGTGTTACTTGACTCCACCTGATACTCTCAAACCAGAAGAGTTTCCTGTGCTGTGCAGATTGGATGCGTGTCGAGTGTCTGTTAAATAA

Protein sequence

>Msa1181800

MGTAGTTKPNLDSILKERKEFDETKAGVKGLVDGGLKKIPSLFHHQPDKHEKANNTSHVIPVVNHGIPLSVLEEMKDGVKRFHEMDTDAKKELYTRDSKRSFVYNSNFDLYSSPALNWRDSFMCYLTPPDTLKPEEFPVLCRLDACRVSVK*