AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1460800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1460800 MtrunA17_Chr1g0203891 99.038 104 1 0 1 104 1 104 5.37e-74 214
Msa1460800 MtrunA17_Chr8g0355101 61.458 96 31 2 6 96 18 112 1.10e-37 122
Msa1460800 MtrunA17_Chr8g0350841 60.417 96 32 2 6 96 18 112 1.14e-35 117
Msa1460800 MtrunA17_Chr7g0266071 63.333 90 31 1 10 97 15 104 2.54e-32 108
Msa1460800 MtrunA17_Chr1g0178891 51.923 104 36 3 2 92 7 109 4.41e-31 106
Msa1460800 MtrunA17_Chr8g0350871 63.043 92 29 1 6 92 18 109 8.04e-31 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1460800 AT4G25433.1 52.525 99 36 3 4 92 7 104 8.69e-30 103
Msa1460800 AT5G62150.1 58.947 95 37 2 7 101 10 102 9.65e-29 100
Msa1460800 AT3G52790.1 51.485 101 37 3 6 96 10 108 3.36e-28 99.0

Find 25 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTATTGGGCATGATATTAT+TGG 0.221140 tig0041091:+9304 Msa1460800:CDS
GTATTGTGCAATAGCGTTAT+TGG 0.257022 tig0041091:+9289 Msa1460800:CDS
CTTCAACAATTTCCTAGTAT+TGG 0.339584 tig0041091:-9543 Msa1460800:intergenic
TTATTGGGCATGATATTATT+GGG 0.350044 tig0041091:+9305 Msa1460800:CDS
GGATTCCTCTCTACAATAAA+CGG 0.377759 tig0041091:-9461 Msa1460800:intergenic
TATTGTGCAATAGCGTTATT+GGG 0.380688 tig0041091:+9290 Msa1460800:CDS
GTGGCTGAATTACAAGTTAG+AGG 0.410773 tig0041091:+9347 Msa1460800:CDS
AGACCAGGGAAAACATCATC+TGG 0.456913 tig0041091:-9497 Msa1460800:intergenic
TCATCACACCGACCAATACT+AGG 0.469317 tig0041091:+9531 Msa1460800:CDS
GGAAAACATCATCTGGATCA+TGG 0.481353 tig0041091:-9490 Msa1460800:intergenic
GATTCCTCTCTACAATAAAC+GGG 0.500635 tig0041091:-9460 Msa1460800:intergenic
CAAGTTAGAGGGAATAGTCT+TGG 0.505805 tig0041091:+9359 Msa1460800:CDS
AACAATTTCCTAGTATTGGT+CGG 0.516529 tig0041091:-9539 Msa1460800:intergenic
TCATCTGGATCATGGATATG+TGG 0.554352 tig0041091:-9482 Msa1460800:intergenic
GAGATATATGTTGTGGGAGA+AGG 0.558851 tig0041091:+9401 Msa1460800:CDS
CACAACATGGGTTCCTCTAG+AGG 0.559347 tig0041091:+9251 None:intergenic
CTAGAGGTGATTCAGCATCA+TGG 0.560521 tig0041091:+9267 Msa1460800:CDS
ATGATTCTGATAACAAGACC+AGG 0.590651 tig0041091:-9512 Msa1460800:intergenic
TGGCTGAATTACAAGTTAGA+GGG 0.596295 tig0041091:+9348 Msa1460800:CDS
TGTGATGAGATATATGTTGT+GGG 0.600497 tig0041091:+9395 Msa1460800:CDS
TGATGCTGAATCACCTCTAG+AGG 0.613640 tig0041091:-9264 Msa1460800:intergenic
GTGACCCGTTTATTGTAGAG+AGG 0.616188 tig0041091:+9456 Msa1460800:CDS
ATGTGATGAGATATATGTTG+TGG 0.646471 tig0041091:+9394 Msa1460800:CDS
ATCAATAAGAGAAAGTACTG+TGG 0.691350 tig0041091:+9328 Msa1460800:CDS
TGATTCTGATAACAAGACCA+GGG 0.737080 tig0041091:-9511 Msa1460790:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTATTGGGCATGATATTATT+GGG + tig0041091:9305-9324 Msa1460800:CDS 25.0%
TATTGTGCAATAGCGTTATT+GGG + tig0041091:9290-9309 Msa1460800:CDS 30.0%
GTTATTGGGCATGATATTAT+TGG + tig0041091:9304-9323 Msa1460800:CDS 30.0%
!! ATCAATAAGAGAAAGTACTG+TGG + tig0041091:9328-9347 Msa1460800:CDS 30.0%
ATGTGATGAGATATATGTTG+TGG + tig0041091:9394-9413 Msa1460800:CDS 30.0%
TGTGATGAGATATATGTTGT+GGG + tig0041091:9395-9414 Msa1460800:CDS 30.0%
AACACAATCAGTGAAAAATG+TGG + tig0041091:9434-9453 Msa1460800:CDS 30.0%
!! AACAATTTCCTAGTATTGGT+CGG - tig0041091:9542-9561 Msa1460800:intergenic 30.0%
CTTCAACAATTTCCTAGTAT+TGG - tig0041091:9546-9565 Msa1460800:intergenic 30.0%
GTATTGTGCAATAGCGTTAT+TGG + tig0041091:9289-9308 Msa1460800:CDS 35.0%
TGGCTGAATTACAAGTTAGA+GGG + tig0041091:9348-9367 Msa1460800:CDS 35.0%
GATTCCTCTCTACAATAAAC+GGG - tig0041091:9463-9482 Msa1460800:intergenic 35.0%
GGATTCCTCTCTACAATAAA+CGG - tig0041091:9464-9483 Msa1460800:intergenic 35.0%
TGATTCTGATAACAAGACCA+GGG - tig0041091:9514-9533 Msa1460800:intergenic 35.0%
ATGATTCTGATAACAAGACC+AGG - tig0041091:9515-9534 Msa1460800:intergenic 35.0%
GTGGCTGAATTACAAGTTAG+AGG + tig0041091:9347-9366 Msa1460800:CDS 40.0%
CAAGTTAGAGGGAATAGTCT+TGG + tig0041091:9359-9378 Msa1460800:CDS 40.0%
GAGATATATGTTGTGGGAGA+AGG + tig0041091:9401-9420 Msa1460800:CDS 40.0%
TCATCTGGATCATGGATATG+TGG - tig0041091:9485-9504 Msa1460800:intergenic 40.0%
GGAAAACATCATCTGGATCA+TGG - tig0041091:9493-9512 Msa1460800:intergenic 40.0%
TGATGCTGAATCACCTCTAG+AGG - tig0041091:9267-9286 Msa0410760:intergenic 45.0%
CTAGAGGTGATTCAGCATCA+TGG + tig0041091:9267-9286 Msa1460800:CDS 45.0%
GTGACCCGTTTATTGTAGAG+AGG + tig0041091:9456-9475 Msa1460800:CDS 45.0%
! GATCCAGATGATGTTTTCCC+TGG + tig0041091:9494-9513 Msa1460800:CDS 45.0%
AGACCAGGGAAAACATCATC+TGG - tig0041091:9500-9519 Msa1460800:intergenic 45.0%
TCATCACACCGACCAATACT+AGG + tig0041091:9531-9550 Msa1460800:CDS 45.0%
Chromosome Type Strat End Strand Name
tig0041091 gene 9257 9571 9257 ID=Msa1460800;Name=Msa1460800
tig0041091 mRNA 9257 9571 9257 ID=Msa1460800-mRNA-1;Parent=Msa1460800;Name=Msa1460800-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|104
tig0041091 exon 9257 9571 9257 ID=Msa1460800-mRNA-1:exon:22896;Parent=Msa1460800-mRNA-1
tig0041091 CDS 9257 9571 9257 ID=Msa1460800-mRNA-1:cds;Parent=Msa1460800-mRNA-1
Gene Sequence

>Msa1460800

ATGGGTTCCTCTAGAGGTGATTCAGCATCATGGTATTGTGCAATAGCGTTATTGGGCATGATATTATTGGGATCAATAAGAGAAAGTACTGTGGCTGAATTACAAGTTAGAGGGAATAGTCTTGGTGATCACAAAGCATGTGATGAGATATATGTTGTGGGAGAAGGAGAAACACTCAACACAATCAGTGAAAAATGTGGTGACCCGTTTATTGTAGAGAGGAATCCACATATCCATGATCCAGATGATGTTTTCCCTGGTCTTGTTATCAGAATCATCACACCGACCAATACTAGGAAATTGTTGAAGAGCTAG

Protein sequence

>Msa1460800

MGSSRGDSASWYCAIALLGMILLGSIRESTVAELQVRGNSLGDHKACDEIYVVGEGETLNTISEKCGDPFIVERNPHIHDPDDVFPGLVIRIITPTNTRKLLKS*