AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1204910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204910 MtrunA17_Chr4g0053841 97.115 208 6 0 1 208 1 208 2.11e-145 417
Msa1204910 MtrunA17_Chr5g0401451 74.038 208 51 2 1 208 28 232 7.09e-107 320
Msa1204910 MtrunA17_Chr1g0206621 89.362 141 15 0 20 160 75 215 8.21e-86 253
Msa1204910 MtrunA17_Chr5g0432861 45.894 207 89 2 1 207 13 196 1.01e-61 201
Msa1204910 MtrunA17_Chr3g0108331 44.928 207 91 2 1 207 12 195 1.84e-60 198
Msa1204910 MtrunA17_Chr4g0010091 44.928 207 108 3 2 208 24 224 4.01e-60 198
Msa1204910 MtrunA17_Chr1g0195911 46.411 209 103 3 1 209 25 224 3.04e-58 193
Msa1204910 MtrunA17_Chr7g0268781 47.867 211 100 5 1 209 25 227 1.15e-57 192
Msa1204910 MtrunA17_Chr4g0050021 44.340 212 110 3 2 207 33 242 6.69e-56 187
Msa1204910 MtrunA17_Chr2g0279481 42.593 216 113 3 1 207 32 245 4.19e-53 180
Msa1204910 MtrunA17_Chr2g0285511 39.336 211 120 4 7 210 20 229 1.35e-47 164
Msa1204910 MtrunA17_Chr7g0245201 42.000 200 103 4 12 208 60 249 4.60e-46 160
Msa1204910 MtrunA17_Chr2g0303541 35.455 220 127 4 2 210 15 230 8.25e-45 157
Msa1204910 MtrunA17_Chr8g0374011 37.991 229 117 5 7 210 14 242 1.94e-44 156
Msa1204910 MtrunA17_Chr1g0150191 39.320 206 117 3 10 209 24 227 3.64e-42 150
Msa1204910 MtrunA17_Chr8g0382231 38.500 200 94 4 13 207 52 227 4.87e-42 149
Msa1204910 MtrunA17_Chr1g0181181 37.255 204 110 3 7 210 20 205 1.42e-40 145
Msa1204910 MtrunA17_Chr1g0207461 35.644 202 123 2 12 208 27 226 1.56e-39 142
Msa1204910 MtrunA17_Chr5g0401341 36.275 204 119 3 12 208 26 225 6.83e-39 141
Msa1204910 MtrunA17_Chr4g0076521 38.191 199 116 2 10 208 23 214 1.54e-38 140
Msa1204910 MtrunA17_Chr2g0290741 36.869 198 116 4 12 208 25 214 2.98e-37 136
Msa1204910 MtrunA17_Chr8g0367891 38.144 194 107 3 16 207 29 211 3.05e-37 136
Msa1204910 MtrunA17_Chr2g0320691 33.014 209 133 2 7 208 17 225 1.76e-35 131
Msa1204910 MtrunA17_Chr1g0191711 31.905 210 120 3 2 209 47 235 1.03e-31 120
Msa1204910 MtrunA17_Chr1g0199631 35.294 187 111 6 31 210 8 191 4.30e-31 119
Msa1204910 MtrunA17_Chr5g0412591 35.407 209 86 5 10 207 23 193 2.07e-27 108
Msa1204910 MtrunA17_Chr4g0037661 28.472 144 98 4 16 155 6 148 2.46e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204910 AT5G67385.1 65.385 208 68 2 1 208 13 216 4.61e-89 273
Msa1204910 AT5G67385.4 64.000 175 59 2 34 208 1 171 3.41e-69 221
Msa1204910 AT5G67385.5 64.000 175 59 2 34 208 1 171 3.41e-69 221
Msa1204910 AT5G67385.2 64.000 175 59 2 34 208 1 171 3.41e-69 221
Msa1204910 AT5G67385.3 64.000 175 59 2 34 208 1 171 3.41e-69 221
Msa1204910 AT2G30520.1 46.411 209 89 2 1 209 18 203 3.58e-63 206
Msa1204910 AT5G48800.1 46.569 204 106 2 5 208 34 234 5.45e-62 203
Msa1204910 AT3G49970.1 53.804 184 60 3 25 208 6 164 5.30e-58 191
Msa1204910 AT3G49970.2 53.804 184 60 3 25 208 6 164 5.30e-58 191
Msa1204910 AT2G47860.4 44.976 209 103 4 1 209 26 222 8.60e-56 183
Msa1204910 AT2G47860.1 44.976 209 103 4 1 209 26 222 1.95e-54 184
Msa1204910 AT1G03010.1 44.498 209 104 3 1 209 25 221 2.15e-52 178
Msa1204910 AT1G03010.2 44.118 204 103 2 6 209 2 194 4.18e-51 174
Msa1204910 AT1G03010.3 44.949 198 98 2 12 209 34 220 7.21e-51 174
Msa1204910 AT3G08570.1 42.289 201 102 3 12 207 33 224 1.08e-50 173
Msa1204910 AT2G47860.6 40.517 232 103 5 1 209 26 245 1.12e-50 171
Msa1204910 AT2G47860.3 40.517 232 103 5 1 209 26 245 1.72e-49 171
Msa1204910 AT5G64330.2 40.265 226 111 4 3 207 42 264 6.77e-49 169
Msa1204910 AT1G30440.1 37.273 220 124 3 2 210 15 231 8.82e-49 169
Msa1204910 AT5G64330.1 40.265 226 111 4 3 207 42 264 1.57e-48 169
Msa1204910 AT2G30520.2 45.198 177 74 2 33 209 2 155 8.46e-47 162
Msa1204910 AT2G30520.3 45.198 177 74 2 33 209 2 155 8.46e-47 162
Msa1204910 AT5G03250.1 36.574 216 124 2 7 210 20 234 4.30e-44 155
Msa1204910 AT4G31820.1 38.119 202 118 3 12 208 26 225 4.71e-44 155
Msa1204910 AT5G67440.3 38.614 202 116 3 10 208 24 220 3.07e-41 147
Msa1204910 AT5G67440.1 38.614 202 116 3 10 208 24 220 3.07e-41 147
Msa1204910 AT5G64330.3 40.201 199 95 4 30 207 10 205 1.92e-39 142
Msa1204910 AT3G08660.2 36.735 196 106 3 12 207 33 210 2.78e-39 140
Msa1204910 AT3G08660.1 36.735 196 106 3 12 207 33 210 1.17e-38 140
Msa1204910 AT4G37590.1 37.019 208 120 2 10 208 24 229 3.41e-37 136
Msa1204910 AT1G67900.5 39.500 200 111 3 10 207 23 214 5.97e-37 136
Msa1204910 AT1G67900.1 39.500 200 111 3 10 207 23 214 5.97e-37 136
Msa1204910 AT1G67900.3 39.500 200 111 3 10 207 23 214 5.97e-37 136
Msa1204910 AT1G67900.6 39.500 200 111 3 10 207 23 214 5.97e-37 136
Msa1204910 AT1G67900.2 39.500 200 111 3 10 207 23 214 5.97e-37 136
Msa1204910 AT1G67900.4 39.500 200 111 3 10 207 23 214 5.97e-37 136
Msa1204910 AT2G14820.2 37.500 200 119 4 12 208 26 222 2.49e-36 134
Msa1204910 AT2G14820.1 37.500 200 119 4 12 208 26 222 2.49e-36 134
Msa1204910 AT5G66560.2 32.296 257 119 6 7 208 13 269 9.09e-36 131
Msa1204910 AT5G67440.2 39.130 184 104 3 28 208 5 183 1.50e-35 131
Msa1204910 AT5G66560.1 32.296 257 119 6 7 208 13 269 2.76e-35 131
Msa1204910 AT5G13600.1 34.597 211 127 5 7 210 20 226 2.95e-35 131
Msa1204910 AT3G26490.2 34.434 212 125 5 10 208 23 233 2.24e-34 127
Msa1204910 AT2G23050.1 35.961 203 124 3 10 209 24 223 3.45e-34 127
Msa1204910 AT3G26490.1 34.434 212 125 5 10 208 23 233 6.07e-34 127
Msa1204910 AT3G26490.3 34.434 212 125 5 10 208 23 233 6.07e-34 127
Msa1204910 AT3G26490.4 34.434 212 125 5 10 208 23 233 6.07e-34 127
Msa1204910 AT3G50840.1 34.300 207 134 2 2 208 5 209 2.15e-33 125
Msa1204910 AT5G10250.1 35.802 162 103 1 2 163 39 199 3.54e-33 125
Msa1204910 AT5G10250.2 35.802 162 103 1 2 163 39 199 4.05e-33 125
Msa1204910 AT4G31820.2 35.632 174 105 3 40 208 1 172 1.50e-31 120
Msa1204910 AT5G48130.1 33.641 217 114 5 7 209 33 233 6.01e-31 119
Msa1204910 AT5G66560.3 32.636 239 106 6 25 208 2 240 1.28e-30 118
Msa1204910 AT3G44820.1 29.767 215 141 4 5 210 15 228 1.57e-30 118
Msa1204910 AT5G47800.3 31.156 199 126 3 12 208 25 214 1.12e-29 115
Msa1204910 AT5G47800.1 31.156 199 126 3 12 208 25 214 1.12e-29 115
Msa1204910 AT5G47800.4 31.156 199 126 3 12 208 25 214 1.12e-29 115
Msa1204910 AT5G47800.2 31.156 199 126 3 12 208 138 327 1.55e-29 115
Msa1204910 AT3G08660.3 36.905 168 88 3 40 207 1 150 1.78e-29 114
Msa1204910 AT5G66560.4 37.725 167 91 4 55 208 64 230 2.39e-28 112
Msa1204910 AT3G50840.2 33.714 175 114 2 34 208 1 173 1.13e-22 95.5
Msa1204910 AT3G50840.3 33.714 175 114 2 34 208 1 173 1.13e-22 95.5
Msa1204910 AT2G47860.5 37.273 110 63 2 100 209 1 104 5.69e-16 76.3
Msa1204910 AT2G47860.2 37.273 110 63 2 100 209 1 104 5.69e-16 76.3
Msa1204910 AT3G49900.1 30.864 162 100 4 3 159 55 209 7.30e-15 72.8
Msa1204910 AT3G49900.2 30.864 162 100 4 3 159 55 209 7.39e-15 72.8
Msa1204910 AT3G19850.1 34.400 125 77 2 33 152 23 147 7.42e-15 72.8
Msa1204910 AT1G50280.1 29.808 208 123 6 15 202 5 209 1.42e-14 72.0
Msa1204910 AT1G50280.2 32.099 162 91 4 57 202 18 176 1.34e-12 66.2
Msa1204910 AT5G17580.2 25.616 203 132 4 15 210 22 212 1.70e-12 66.2
Msa1204910 AT5G17580.1 25.616 203 132 4 15 210 7 197 2.45e-12 65.5
Msa1204910 AT5G03250.2 30.081 123 73 2 100 210 1 122 1.07e-11 63.5

Find 122 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAATTTGAGCCACATTT+TGG 0.185225 8_1:-60760774 None:intergenic
GTCTAGTTGTATGCGATCTT+TGG 0.210752 8_1:-60761160 None:intergenic
CTTCCTTATGTGTCCACTTT+TGG 0.213031 8_1:-60758406 None:intergenic
CCATTCACTTGTTCTCTTCA+TGG 0.260781 8_1:-60757919 None:intergenic
AGATTATTCGGTCGGAAATT+TGG 0.273291 8_1:+60758608 Msa1204910:CDS
CTGTCAGCTTTCTTTCACTA+TGG 0.279955 8_1:+60759750 Msa1204910:three_prime_UTR
TGTGAATAAAGCATTATAAT+AGG 0.301041 8_1:-60758977 None:intergenic
GAGTTTAGGAATGACTTTCT+AGG 0.305347 8_1:-60761075 None:intergenic
CTACAATGTGACTGTGATTA+AGG 0.310866 8_1:-60757842 None:intergenic
TTGAGACAACTGTTGCTTGC+AGG 0.327110 8_1:+60759792 Msa1204910:three_prime_UTR
TTTGTAAGATATTTATTTGC+AGG 0.329856 8_1:+60761323 Msa1204910:three_prime_UTR
ATAAAACCCTTTGCGGTTGC+GGG 0.357820 8_1:+60757875 Msa1204910:five_prime_UTR
AGTGATGTCGCTATTCAAAT+TGG 0.360026 8_1:+60758077 Msa1204910:CDS
TGTTGCTTGCAGGTTTGATT+TGG 0.361308 8_1:+60759802 Msa1204910:three_prime_UTR
AAAATATCTTGCACAAAATC+AGG 0.361655 8_1:-60761205 None:intergenic
CATTCTTCTCCCTTGGCATA+AGG 0.363104 8_1:-60759721 None:intergenic
CTCCTTCCCGCAACCGCAAA+GGG 0.363358 8_1:-60757881 None:intergenic
ATTGAACTCATCGATGTTCC+TGG 0.366170 8_1:+60758471 Msa1204910:CDS
TCTTGCACAAAATCAGGATA+TGG 0.373271 8_1:-60761199 None:intergenic
TGAAATGACAGAAGATTATT+CGG 0.380236 8_1:+60758596 Msa1204910:CDS
CTTTCTTTCACTATGGCTTA+AGG 0.386758 8_1:+60759757 Msa1204910:three_prime_UTR
TCTCTTCATGGCACTAGAAA+TGG 0.387904 8_1:-60757907 None:intergenic
GCGCCTCTCGTGATAGCTTC+TGG 0.389792 8_1:-60760717 None:intergenic
CATAAAACCCTTTGCGGTTG+CGG 0.392282 8_1:+60757874 Msa1204910:five_prime_UTR
TATGCATGGGAAATGTTAAC+CGG 0.392483 8_1:+60761286 Msa1204910:three_prime_UTR
ACATCGATGAGTTCAATTAA+TGG 0.394415 8_1:-60758465 None:intergenic
AGAAGGATAGCCATGCAATT+AGG 0.395560 8_1:+60759827 Msa1204910:three_prime_UTR
TCTAGTTGTATGCGATCTTT+GGG 0.404869 8_1:-60761159 None:intergenic
TTTAACAAAGGACTATTGAT+AGG 0.405011 8_1:-60761018 None:intergenic
AAGAGTTCTAATTGCAATGA+TGG 0.405470 8_1:+60758925 Msa1204910:exon
TTTCCATTAATTTCCAAAAG+TGG 0.409367 8_1:+60758393 Msa1204910:CDS
GCTTCCAAGGAGAGTCAGTT+TGG 0.413724 8_1:+60758777 Msa1204910:CDS
GTTGCACTAAAGAGCATGTC+AGG 0.420005 8_1:+60758660 Msa1204910:CDS
GAGAGTCAGTTTGGTTCATC+TGG 0.422018 8_1:+60758786 Msa1204910:CDS
ACAGAAATCTCTGAGAGGTT+TGG 0.436903 8_1:+60758997 Msa1204910:three_prime_UTR
TTTCCAAAAGTGGACACATA+AGG 0.437270 8_1:+60758403 Msa1204910:CDS
ACCAATCAACAGCTGGCCTC+TGG 0.440922 8_1:-60758863 None:intergenic
CGACATCACTTGGAATCTCC+TGG 0.445103 8_1:-60758064 None:intergenic
TTCCTCTTGTCAGGTGTTGA+AGG 0.446976 8_1:-60760859 None:intergenic
ACCAGAGGCCAGCTGTTGAT+TGG 0.449238 8_1:+60758862 Msa1204910:CDS
GAGTATACATTCCTCTTGTC+AGG 0.452843 8_1:-60760868 None:intergenic
AGCCGTAGACATCTATCTTA+AGG 0.453245 8_1:+60760144 Msa1204910:three_prime_UTR
TAGCAGCAAAGTTCTGTTAC+GGG 0.457361 8_1:+60758514 Msa1204910:CDS
AACTACCTTGTGTAACGAAT+AGG 0.461457 8_1:-60758108 None:intergenic
GATATTGCATTCTTCTCCCT+TGG 0.461512 8_1:-60759728 None:intergenic
AGAGGCCAGCTGTTGATTGG+TGG 0.462714 8_1:+60758865 Msa1204910:CDS
CCCACGCTGCACAAAACAAC+AGG 0.463975 8_1:+60760743 Msa1204910:three_prime_UTR
CGGATACATAAAACCCTTTG+CGG 0.464676 8_1:+60757868 Msa1204910:five_prime_UTR
CTGTCCGTGTCGCTTACAAA+TGG 0.464751 8_1:-60760667 None:intergenic
AACTCAGTTTCAAGAAAACT+TGG 0.478198 8_1:+60761093 Msa1204910:three_prime_UTR
CCTCCTTCCCGCAACCGCAA+AGG 0.478959 8_1:-60757882 None:intergenic
AAATCAAGAACAACAGAAGA+TGG 0.483167 8_1:+60760112 Msa1204910:three_prime_UTR
AAAGAAAGTTTGCAGCATGA+TGG 0.486600 8_1:+60760690 Msa1204910:three_prime_UTR
ACTCTCCTTGGAAGCTATGT+AGG 0.486973 8_1:-60758769 None:intergenic
TGAACCAAACTGACTCTCCT+TGG 0.490716 8_1:-60758781 None:intergenic
ACAGGATGAGCACGTTGAAA+AGG 0.498236 8_1:-60761126 None:intergenic
GATGTGTCGCCGCTAAATGA+AGG 0.498482 8_1:-60759878 None:intergenic
GTATTATTCTTCTCCACAAA+GGG 0.500177 8_1:+60759982 Msa1204910:three_prime_UTR
AGGAATGACTTTCTAGGCAA+AGG 0.500562 8_1:-60761069 None:intergenic
ATTTGAATAGCGACATCACT+TGG 0.501604 8_1:-60758074 None:intergenic
TTGGAAACCTCATTAGAAGC+TGG 0.504132 8_1:-60760904 None:intergenic
CTAGCAGCAAAGTTCTGTTA+CGG 0.504241 8_1:+60758513 Msa1204910:CDS
TTCTGTCATTTCAAGATACT+CGG 0.511486 8_1:-60758586 None:intergenic
CTCTTCTCATGCTCTTCATG+TGG 0.516105 8_1:-60759674 None:intergenic
GATATTTGGAAAGGGAAGGA+AGG 0.520414 8_1:+60759643 Msa1204910:three_prime_UTR
GAGCAGGAGATATTTGGAAA+GGG 0.520665 8_1:+60759635 Msa1204910:intron
AGTTTCTGATGTATGTAAGA+TGG 0.522762 8_1:-60758691 None:intergenic
GCTTTATCCAGCTTCTAATG+AGG 0.525232 8_1:+60760897 Msa1204910:three_prime_UTR
GAGGCCAGCTGTTGATTGGT+GGG 0.529140 8_1:+60758866 Msa1204910:CDS
TCACAGTCACATTGTAGTCT+CGG 0.533562 8_1:+60757848 Msa1204910:five_prime_UTR
ATGGATGGCACTGGCAGCAT+AGG 0.533950 8_1:+60760826 Msa1204910:three_prime_UTR
CATCGCTATGCTTCGATGTG+TGG 0.533993 8_1:+60758563 Msa1204910:CDS
TTGTAAGATATTTATTTGCA+GGG 0.534022 8_1:+60761324 Msa1204910:three_prime_UTR
GGTTCATCTGGTAGAAGTGA+TGG 0.534039 8_1:+60758798 Msa1204910:CDS
AACCCTTTGCGGTTGCGGGA+AGG 0.540883 8_1:+60757879 Msa1204910:five_prime_UTR
TCTTTGGGACGTGCTATGAC+AGG 0.544164 8_1:-60761144 None:intergenic
TCAGCCCACCAATCAACAGC+TGG 0.545487 8_1:-60758870 None:intergenic
GGAGCAGGAGATATTTGGAA+AGG 0.547365 8_1:+60759634 Msa1204910:intron
TATTCGGTCGGAAATTTGGT+AGG 0.547468 8_1:+60758612 Msa1204910:CDS
CGCCTTCAACACCTGACAAG+AGG 0.552605 8_1:+60760857 Msa1204910:three_prime_UTR
TTATCACAGGAATTACTCCC+AGG 0.555974 8_1:+60758046 Msa1204910:intron
GATAGCCATGCAATTAGGAC+AGG 0.556430 8_1:+60759832 Msa1204910:three_prime_UTR
AGGAGATATTTGGAAAGGGA+AGG 0.557338 8_1:+60759639 Msa1204910:intron
AATAGCCTACATAGCTTCCA+AGG 0.561754 8_1:+60758764 Msa1204910:CDS
CCATGAAGAGAACAAGTGAA+TGG 0.562517 8_1:+60757919 Msa1204910:exon
GCAATTAGGACAGGCTGTGT+TGG 0.562742 8_1:+60759841 Msa1204910:three_prime_UTR
GCAGGTTTGATTTGGAGAGA+AGG 0.563785 8_1:+60759810 Msa1204910:three_prime_UTR
GCAACGTCTTCGCGACAACA+TGG 0.564907 8_1:+60760807 Msa1204910:three_prime_UTR
TACTAGTGAGCCTTATGCCA+AGG 0.566316 8_1:+60759711 Msa1204910:three_prime_UTR
CACAGCCTGTCCTAATTGCA+TGG 0.571835 8_1:-60759837 None:intergenic
CCTTTGCGGTTGCGGGAAGG+AGG 0.572447 8_1:+60757882 Msa1204910:five_prime_UTR
CGCGACAACATGGATGGCAC+TGG 0.572937 8_1:+60760817 Msa1204910:three_prime_UTR
AGTATTATTCTTCTCCACAA+AGG 0.574490 8_1:+60759981 Msa1204910:three_prime_UTR
CTAATTGCAATGATGGCTAG+AGG 0.579105 8_1:+60758932 Msa1204910:exon
ATGACAGAAGATTATTCGGT+CGG 0.582614 8_1:+60758600 Msa1204910:CDS
ATGCATGGGAAATGTTAACC+GGG 0.582676 8_1:+60761287 Msa1204910:three_prime_UTR
GACATCACTTGGAATCTCCT+GGG 0.585133 8_1:-60758063 None:intergenic
TGGCCTCTGGTGAGAAGCAG+TGG 0.585433 8_1:-60758850 None:intergenic
ATTAGAAGCTGGATAAAGCT+CGG 0.591749 8_1:-60760893 None:intergenic
CAGGCTTCCTGCCCAAAATG+TGG 0.597481 8_1:+60760762 Msa1204910:three_prime_UTR
GGGAAATGTTAACCGGGGAA+AGG 0.597771 8_1:+60761293 Msa1204910:three_prime_UTR
TAATTGCAATGATGGCTAGA+GGG 0.601649 8_1:+60758933 Msa1204910:exon
AAAGGAGGCTTATCAGCTGA+AGG 0.602067 8_1:-60761051 None:intergenic
CGTCTTCGCGACAACATGGA+TGG 0.604136 8_1:+60760811 Msa1204910:three_prime_UTR
TACCTTAAGATAGATGTCTA+CGG 0.604585 8_1:-60760146 None:intergenic
CTTCCACTGCTTCTCACCAG+AGG 0.605670 8_1:+60758847 Msa1204910:CDS
GTTGTGCGTGATATTATGCA+TGG 0.609354 8_1:+60761272 Msa1204910:three_prime_UTR
CACGTTGAAAAGGAGAAAGA+CGG 0.618103 8_1:-60761116 None:intergenic
GGTGATGATGCATTTAACAA+AGG 0.620143 8_1:-60761030 None:intergenic
AGCTTCCTATTCGTTACACA+AGG 0.621103 8_1:+60758103 Msa1204910:CDS
ACTAGTGAGCCTTATGCCAA+GGG 0.621863 8_1:+60759712 Msa1204910:three_prime_UTR
GAACTCATCGATGTTCCTGG+TGG 0.647010 8_1:+60758474 Msa1204910:CDS
TTGCACTAAAGAGCATGTCA+GGG 0.650226 8_1:+60758661 Msa1204910:CDS
TATCAGTAGCTATTTCAGCA+AGG 0.650241 8_1:-60760039 None:intergenic
AATGACTTTCTAGGCAAAGG+AGG 0.650612 8_1:-60761066 None:intergenic
TCAAATGCTTCTGATCCACC+AGG 0.656366 8_1:-60758489 None:intergenic
TGCACTAAAGAGCATGTCAG+GGG 0.667865 8_1:+60758662 Msa1204910:CDS
TTGTGCGTGATATTATGCAT+GGG 0.675438 8_1:+60761273 Msa1204910:three_prime_UTR
TTTCCAGAAGCTATCACGAG+AGG 0.681776 8_1:+60760714 Msa1204910:three_prime_UTR
TATTCACAGAAATCTCTGAG+AGG 0.685412 8_1:+60758992 Msa1204910:three_prime_UTR
TGCATGGGAAATGTTAACCG+GGG 0.704804 8_1:+60761288 Msa1204910:three_prime_UTR
ACATCCATTTGTAAGCGACA+CGG 0.708353 8_1:+60760663 Msa1204910:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTATCAAGAA+TGG + chr8_1:60758281-60758300 Msa1204910:intron 10.0%
!! TAAAATAAAAATAAAAGAGA+CGG + chr8_1:60760972-60760991 Msa1204910:three_prime_UTR 10.0%
!! TAAATATAAGTTTATAAGTG+AGG - chr8_1:60759462-60759481 None:intergenic 15.0%
!! TCACTTATAAACTTATATTT+AGG + chr8_1:60759461-60759480 Msa1204910:intron 15.0%
!!! TTATATTTTATCTAAACTAG+TGG - chr8_1:60759527-60759546 None:intergenic 15.0%
!!! CAAGATATTTTTATCTTTTT+TGG + chr8_1:60761217-60761236 Msa1204910:three_prime_UTR 15.0%
!! AAAAAACGTTATATTAGCAT+CGG - chr8_1:60757985-60758004 None:intergenic 20.0%
!!! TTTTTATCAAGAATGGTAAT+AGG + chr8_1:60758288-60758307 Msa1204910:intron 20.0%
!!! TGTGAATAAAGCATTATAAT+AGG - chr8_1:60758980-60758999 None:intergenic 20.0%
!!! AATTTTCCTTCTTTAGTAAA+GGG - chr8_1:60759028-60759047 None:intergenic 20.0%
!!! AAATTTTCCTTCTTTAGTAA+AGG - chr8_1:60759029-60759048 None:intergenic 20.0%
!!! TTTCATATCAGTTTTGTTTT+AGG + chr8_1:60759047-60759066 Msa1204910:intron 20.0%
!!! AAAATTTTAAGATGGTATCA+GGG - chr8_1:60759387-60759406 None:intergenic 20.0%
!! AATTCTAACTTTATTAGCAT+TGG - chr8_1:60760629-60760648 None:intergenic 20.0%
!!! TTCATTTTCTCTTCTTAATT+TGG - chr8_1:60760926-60760945 None:intergenic 20.0%
!! TTTGTAAGATATTTATTTGC+AGG + chr8_1:60761323-60761342 Msa1204910:three_prime_UTR 20.0%
!! TTGTAAGATATTTATTTGCA+GGG + chr8_1:60761324-60761343 Msa1204910:three_prime_UTR 20.0%
!!! GATGCTAATATAACGTTTTT+TGG + chr8_1:60757984-60758003 Msa1204910:intron 25.0%
! TGATAAGTAGTTCAATTCAA+AGG - chr8_1:60758032-60758051 None:intergenic 25.0%
! ACAAACTCAAATATCAAACT+TGG + chr8_1:60758132-60758151 Msa1204910:intron 25.0%
! TTTCCATTAATTTCCAAAAG+TGG + chr8_1:60758393-60758412 Msa1204910:CDS 25.0%
! TGAAATGACAGAAGATTATT+CGG + chr8_1:60758596-60758615 Msa1204910:CDS 25.0%
! TGAAAATACCTTTATAAGGT+AGG - chr8_1:60759220-60759239 None:intergenic 25.0%
!!! TACCTTATAAAGGTATTTTC+AGG + chr8_1:60759219-60759238 Msa1204910:intron 25.0%
!!! CAAAATTTTAAGATGGTATC+AGG - chr8_1:60759388-60759407 None:intergenic 25.0%
!! CTGATACCATCTTAAAATTT+TGG + chr8_1:60759386-60759405 Msa1204910:intron 25.0%
!! ACCATCTTAAAATTTTGGAT+TGG + chr8_1:60759391-60759410 Msa1204910:intron 25.0%
!! CCATCTTAAAATTTTGGATT+GGG + chr8_1:60759392-60759411 Msa1204910:intron 25.0%
! TATAAGTTTATAAGTGAGGT+CGG - chr8_1:60759458-60759477 None:intergenic 25.0%
! AAGATTTGATTAGATGTATG+TGG - chr8_1:60759487-60759506 None:intergenic 25.0%
!!! ATTGAACCTTGACTATTTTT+TGG + chr8_1:60759613-60759632 Msa1204910:intron 25.0%
! ATTTACGACAACAATTTAGA+TGG + chr8_1:60760232-60760251 Msa1204910:intron 25.0%
! AAAATTGTGGTTAAGTATGA+TGG - chr8_1:60760334-60760353 None:intergenic 25.0%
! CAATTTGTGTTCAAAAATTG+TGG - chr8_1:60760347-60760366 None:intergenic 25.0%
!! CAATTTTTGAACACAAATTG+TGG + chr8_1:60760347-60760366 Msa1204910:intron 25.0%
! AGTATTATCCTTGAAATCAA+AGG + chr8_1:60760558-60760577 Msa1204910:intron 25.0%
! AGTTAGAATTCAAAATTCTC+AGG + chr8_1:60760639-60760658 Msa1204910:intron 25.0%
! TTTAACAAAGGACTATTGAT+AGG - chr8_1:60761021-60761040 None:intergenic 25.0%
! AAAATATCTTGCACAAAATC+AGG - chr8_1:60761208-60761227 None:intergenic 25.0%
! TATATGTATTTTGTGCTGAG+TGG - chr8_1:60757783-60757802 None:intergenic 30.0%
TGAATTGAACTACTTATCAC+AGG + chr8_1:60758033-60758052 Msa1204910:intron 30.0%
TGGTTATGAATTCTAGTACT+TGG + chr8_1:60758152-60758171 Msa1204910:intron 30.0%
!! TGTCCACTTTTGGAAATTAA+TGG - chr8_1:60758399-60758418 None:intergenic 30.0%
ACATCGATGAGTTCAATTAA+TGG - chr8_1:60758468-60758487 None:intergenic 30.0%
! TTCTGTCATTTCAAGATACT+CGG - chr8_1:60758589-60758608 None:intergenic 30.0%
AGTTTCTGATGTATGTAAGA+TGG - chr8_1:60758694-60758713 None:intergenic 30.0%
! AATTTTGCTTTCTCTGCAAT+TGG - chr8_1:60758723-60758742 None:intergenic 30.0%
! AAGAGTTCTAATTGCAATGA+TGG + chr8_1:60758925-60758944 Msa1204910:exon 30.0%
GGGTTTAAAAATTGTGGTAT+TGG + chr8_1:60758953-60758972 Msa1204910:three_prime_UTR 30.0%
GACCTGAAAATACCTTTATA+AGG - chr8_1:60759224-60759243 None:intergenic 30.0%
!! CCCAATCCAAAATTTTAAGA+TGG - chr8_1:60759395-60759414 None:intergenic 30.0%
! CTTCTTTTTCCTTCATTTAG+CGG + chr8_1:60759869-60759888 Msa1204910:three_prime_UTR 30.0%
! GATTTTCAACTTCAGATTCA+AGG - chr8_1:60759943-60759962 None:intergenic 30.0%
AGTATTATTCTTCTCCACAA+AGG + chr8_1:60759981-60760000 Msa1204910:three_prime_UTR 30.0%
GTATTATTCTTCTCCACAAA+GGG + chr8_1:60759982-60760001 Msa1204910:three_prime_UTR 30.0%
! TCTGTTGTTCTTGATTTTTC+AGG - chr8_1:60760109-60760128 None:intergenic 30.0%
AAATCAAGAACAACAGAAGA+TGG + chr8_1:60760112-60760131 Msa1204910:three_prime_UTR 30.0%
TACCTTAAGATAGATGTCTA+CGG - chr8_1:60760149-60760168 None:intergenic 30.0%
GACGATTATTGAGTATAACA+AGG + chr8_1:60760293-60760312 Msa1204910:intron 30.0%
GTAAGTTCAATTCTGTTATG+TGG - chr8_1:60760535-60760554 None:intergenic 30.0%
! AGTTAAGTCCTTTGATTTCA+AGG - chr8_1:60760569-60760588 None:intergenic 30.0%
! GTTTTCTTGAAACTGAGTTT+AGG - chr8_1:60761092-60761111 None:intergenic 30.0%
AACTCAGTTTCAAGAAAACT+TGG + chr8_1:60761093-60761112 Msa1204910:three_prime_UTR 30.0%
!!! GTTTTGTTTTGTTCACTTAG+TGG + chr8_1:60761352-60761371 Msa1204910:three_prime_UTR 30.0%
! TGCTTTTATGAGAGTTTGAG+TGG - chr8_1:60757756-60757775 None:intergenic 35.0%
CTACAATGTGACTGTGATTA+AGG - chr8_1:60757845-60757864 None:intergenic 35.0%
ATTTGAATAGCGACATCACT+TGG - chr8_1:60758077-60758096 None:intergenic 35.0%
AGTGATGTCGCTATTCAAAT+TGG + chr8_1:60758077-60758096 Msa1204910:CDS 35.0%
AACTACCTTGTGTAACGAAT+AGG - chr8_1:60758111-60758130 None:intergenic 35.0%
ACTAAGAAACAACCAATACG+CGG - chr8_1:60758253-60758272 None:intergenic 35.0%
TTTCCAAAAGTGGACACATA+AGG + chr8_1:60758403-60758422 Msa1204910:CDS 35.0%
ATGACAGAAGATTATTCGGT+CGG + chr8_1:60758600-60758619 Msa1204910:CDS 35.0%
AGATTATTCGGTCGGAAATT+TGG + chr8_1:60758608-60758627 Msa1204910:CDS 35.0%
TAATTGCAATGATGGCTAGA+GGG + chr8_1:60758933-60758952 Msa1204910:exon 35.0%
GCTAGAGGGTTTAAAAATTG+TGG + chr8_1:60758947-60758966 Msa1204910:three_prime_UTR 35.0%
TATTCACAGAAATCTCTGAG+AGG + chr8_1:60758992-60759011 Msa1204910:three_prime_UTR 35.0%
GTAAGTCCCTTTACTAAAGA+AGG + chr8_1:60759019-60759038 Msa1204910:intron 35.0%
CCTTTATAAGGTAGGTAGTT+CGG - chr8_1:60759212-60759231 None:intergenic 35.0%
!!! TTTTTGGAGCAGGAGATATT+TGG + chr8_1:60759629-60759648 Msa1204910:intron 35.0%
CTTTCTTTCACTATGGCTTA+AGG + chr8_1:60759757-60759776 Msa1204910:three_prime_UTR 35.0%
! CTCCACAAAGGGATATTTTT+CGG + chr8_1:60759993-60760012 Msa1204910:three_prime_UTR 35.0%
! TCCACAAAGGGATATTTTTC+GGG + chr8_1:60759994-60760013 Msa1204910:three_prime_UTR 35.0%
!!! TTTTCGGGTTGGAAAGTTAT+TGG + chr8_1:60760009-60760028 Msa1204910:three_prime_UTR 35.0%
! TATCAGTAGCTATTTCAGCA+AGG - chr8_1:60760042-60760061 None:intergenic 35.0%
GCTAAAGCAGTTCAAATATG+TGG + chr8_1:60760254-60760273 Msa1204910:intron 35.0%
AATCTGTGTTCGCATATAGA+TGG - chr8_1:60760401-60760420 None:intergenic 35.0%
ATATGCGAACACAGATTAGA+TGG + chr8_1:60760404-60760423 Msa1204910:intron 35.0%
! CGTCGTTAACTATGATTTTC+AGG - chr8_1:60760462-60760481 None:intergenic 35.0%
GTTAACGACGTTCAAAATCA+AGG + chr8_1:60760472-60760491 Msa1204910:intron 35.0%
AAAGAAAGTTTGCAGCATGA+TGG + chr8_1:60760690-60760709 Msa1204910:three_prime_UTR 35.0%
! GAGAATTTGAGCCACATTTT+GGG - chr8_1:60760776-60760795 None:intergenic 35.0%
! AGAGAATTTGAGCCACATTT+TGG - chr8_1:60760777-60760796 None:intergenic 35.0%
ATTAGAAGCTGGATAAAGCT+CGG - chr8_1:60760896-60760915 None:intergenic 35.0%
! GGTGATGATGCATTTAACAA+AGG - chr8_1:60761033-60761052 None:intergenic 35.0%
GAGTTTAGGAATGACTTTCT+AGG - chr8_1:60761078-60761097 None:intergenic 35.0%
TCTAGTTGTATGCGATCTTT+GGG - chr8_1:60761162-60761181 None:intergenic 35.0%
TCTTGCACAAAATCAGGATA+TGG - chr8_1:60761202-60761221 None:intergenic 35.0%
TTGTGCGTGATATTATGCAT+GGG + chr8_1:60761273-60761292 Msa1204910:three_prime_UTR 35.0%
TATGCATGGGAAATGTTAAC+CGG + chr8_1:60761286-60761305 Msa1204910:three_prime_UTR 35.0%
! ACAAATGTTTTACCTTTCCC+CGG - chr8_1:60761308-60761327 None:intergenic 35.0%
TCACAGTCACATTGTAGTCT+CGG + chr8_1:60757848-60757867 Msa1204910:five_prime_UTR 40.0%
CGGATACATAAAACCCTTTG+CGG + chr8_1:60757868-60757887 Msa1204910:five_prime_UTR 40.0%
TCTCTTCATGGCACTAGAAA+TGG - chr8_1:60757910-60757929 None:intergenic 40.0%
CCATTCACTTGTTCTCTTCA+TGG - chr8_1:60757922-60757941 None:intergenic 40.0%
CCATGAAGAGAACAAGTGAA+TGG + chr8_1:60757919-60757938 Msa1204910:exon 40.0%
ACAAGTGAATGGTATGCTTC+TGG + chr8_1:60757930-60757949 Msa1204910:intron 40.0%
TTATCACAGGAATTACTCCC+AGG + chr8_1:60758046-60758065 Msa1204910:intron 40.0%
AGCTTCCTATTCGTTACACA+AGG + chr8_1:60758103-60758122 Msa1204910:CDS 40.0%
! ATTTAGTTGACTCCGCGTAT+TGG + chr8_1:60758238-60758257 Msa1204910:intron 40.0%
! CTTCCTTATGTGTCCACTTT+TGG - chr8_1:60758409-60758428 None:intergenic 40.0%
ATTGAACTCATCGATGTTCC+TGG + chr8_1:60758471-60758490 Msa1204910:CDS 40.0%
CTAGCAGCAAAGTTCTGTTA+CGG + chr8_1:60758513-60758532 Msa1204910:CDS 40.0%
TAGCAGCAAAGTTCTGTTAC+GGG + chr8_1:60758514-60758533 Msa1204910:CDS 40.0%
TATTCGGTCGGAAATTTGGT+AGG + chr8_1:60758612-60758631 Msa1204910:CDS 40.0%
! TTGCACTAAAGAGCATGTCA+GGG + chr8_1:60758661-60758680 Msa1204910:CDS 40.0%
AATAGCCTACATAGCTTCCA+AGG + chr8_1:60758764-60758783 Msa1204910:CDS 40.0%
CTAATTGCAATGATGGCTAG+AGG + chr8_1:60758932-60758951 Msa1204910:exon 40.0%
! ACAGAAATCTCTGAGAGGTT+TGG + chr8_1:60758997-60759016 Msa1204910:three_prime_UTR 40.0%
TAAGATGAGTAGCCTGATAG+TGG + chr8_1:60759166-60759185 Msa1204910:intron 40.0%
AAATCCAGATGTCCACTATC+AGG - chr8_1:60759181-60759200 None:intergenic 40.0%
ATAAGGTAGGTAGTTCGGTA+AGG - chr8_1:60759207-60759226 None:intergenic 40.0%
CCGAACTACCTACCTTATAA+AGG + chr8_1:60759209-60759228 Msa1204910:intron 40.0%
GTCTGTTAAGAGTCTCACAT+TGG - chr8_1:60759256-60759275 None:intergenic 40.0%
GGGTCTATCCAATATCTGTT+GGG - chr8_1:60759367-60759386 None:intergenic 40.0%
AGGGTCTATCCAATATCTGT+TGG - chr8_1:60759368-60759387 None:intergenic 40.0%
TCTTACAGAACCAGCTTGTA+AGG + chr8_1:60759426-60759445 Msa1204910:intron 40.0%
CCTGCTCCAAAAAATAGTCA+AGG - chr8_1:60759622-60759641 None:intergenic 40.0%
!!! CCTTGACTATTTTTTGGAGC+AGG + chr8_1:60759619-60759638 Msa1204910:intron 40.0%
GAGCAGGAGATATTTGGAAA+GGG + chr8_1:60759635-60759654 Msa1204910:intron 40.0%
AGGAGATATTTGGAAAGGGA+AGG + chr8_1:60759639-60759658 Msa1204910:intron 40.0%
GATATTTGGAAAGGGAAGGA+AGG + chr8_1:60759643-60759662 Msa1204910:three_prime_UTR 40.0%
GATATTGCATTCTTCTCCCT+TGG - chr8_1:60759731-60759750 None:intergenic 40.0%
CTGTCAGCTTTCTTTCACTA+TGG + chr8_1:60759750-60759769 Msa1204910:three_prime_UTR 40.0%
! TGTTGCTTGCAGGTTTGATT+TGG + chr8_1:60759802-60759821 Msa1204910:three_prime_UTR 40.0%
AGAAGGATAGCCATGCAATT+AGG + chr8_1:60759827-60759846 Msa1204910:three_prime_UTR 40.0%
ACCCGAAAAATATCCCTTTG+TGG - chr8_1:60759998-60760017 None:intergenic 40.0%
!!! CAAAGGGATATTTTTCGGGT+TGG + chr8_1:60759998-60760017 Msa1204910:three_prime_UTR 40.0%
AGCCGTAGACATCTATCTTA+AGG + chr8_1:60760144-60760163 Msa1204910:three_prime_UTR 40.0%
ACATCCATTTGTAAGCGACA+CGG + chr8_1:60760663-60760682 Msa1204910:three_prime_UTR 40.0%
GAGTATACATTCCTCTTGTC+AGG - chr8_1:60760871-60760890 None:intergenic 40.0%
GCTTTATCCAGCTTCTAATG+AGG + chr8_1:60760897-60760916 Msa1204910:three_prime_UTR 40.0%
TTGGAAACCTCATTAGAAGC+TGG - chr8_1:60760907-60760926 None:intergenic 40.0%
AATGACTTTCTAGGCAAAGG+AGG - chr8_1:60761069-60761088 None:intergenic 40.0%
AGGAATGACTTTCTAGGCAA+AGG - chr8_1:60761072-60761091 None:intergenic 40.0%
CACGTTGAAAAGGAGAAAGA+CGG - chr8_1:60761119-60761138 None:intergenic 40.0%
GTCTAGTTGTATGCGATCTT+TGG - chr8_1:60761163-60761182 None:intergenic 40.0%
GTTGTGCGTGATATTATGCA+TGG + chr8_1:60761272-60761291 Msa1204910:three_prime_UTR 40.0%
ATGCATGGGAAATGTTAACC+GGG + chr8_1:60761287-60761306 Msa1204910:three_prime_UTR 40.0%
CATAAAACCCTTTGCGGTTG+CGG + chr8_1:60757874-60757893 Msa1204910:five_prime_UTR 45.0%
ATAAAACCCTTTGCGGTTGC+GGG + chr8_1:60757875-60757894 Msa1204910:five_prime_UTR 45.0%
GACATCACTTGGAATCTCCT+GGG - chr8_1:60758066-60758085 None:intergenic 45.0%
TCAAATGCTTCTGATCCACC+AGG - chr8_1:60758492-60758511 None:intergenic 45.0%
! GTTGCACTAAAGAGCATGTC+AGG + chr8_1:60758660-60758679 Msa1204910:CDS 45.0%
! TGCACTAAAGAGCATGTCAG+GGG + chr8_1:60758662-60758681 Msa1204910:CDS 45.0%
ACTCTCCTTGGAAGCTATGT+AGG - chr8_1:60758772-60758791 None:intergenic 45.0%
! TGAACCAAACTGACTCTCCT+TGG - chr8_1:60758784-60758803 None:intergenic 45.0%
! GAGAGTCAGTTTGGTTCATC+TGG + chr8_1:60758786-60758805 Msa1204910:CDS 45.0%
GGTTCATCTGGTAGAAGTGA+TGG + chr8_1:60758798-60758817 Msa1204910:CDS 45.0%
!! CGCTCTAGATGTCCTGTTTT+GGG - chr8_1:60759303-60759322 None:intergenic 45.0%
!! ACGCTCTAGATGTCCTGTTT+TGG - chr8_1:60759304-60759323 None:intergenic 45.0%
GAAAGTGGCCCAACAGATAT+TGG + chr8_1:60759356-60759375 Msa1204910:intron 45.0%
GGAGCAGGAGATATTTGGAA+AGG + chr8_1:60759634-60759653 Msa1204910:intron 45.0%
CTCTTCTCATGCTCTTCATG+TGG - chr8_1:60759677-60759696 None:intergenic 45.0%
! TACTAGTGAGCCTTATGCCA+AGG + chr8_1:60759711-60759730 Msa1204910:three_prime_UTR 45.0%
! ACTAGTGAGCCTTATGCCAA+GGG + chr8_1:60759712-60759731 Msa1204910:three_prime_UTR 45.0%
CATTCTTCTCCCTTGGCATA+AGG - chr8_1:60759724-60759743 None:intergenic 45.0%
! TTGAGACAACTGTTGCTTGC+AGG + chr8_1:60759792-60759811 Msa1204910:three_prime_UTR 45.0%
!! GCAGGTTTGATTTGGAGAGA+AGG + chr8_1:60759810-60759829 Msa1204910:three_prime_UTR 45.0%
GATAGCCATGCAATTAGGAC+AGG + chr8_1:60759832-60759851 Msa1204910:three_prime_UTR 45.0%
TTTCCAGAAGCTATCACGAG+AGG + chr8_1:60760714-60760733 Msa1204910:three_prime_UTR 45.0%
! ATTTGAGCCACATTTTGGGC+AGG - chr8_1:60760772-60760791 None:intergenic 45.0%
!! TTCCTCTTGTCAGGTGTTGA+AGG - chr8_1:60760862-60760881 None:intergenic 45.0%
AAAGGAGGCTTATCAGCTGA+AGG - chr8_1:60761054-60761073 None:intergenic 45.0%
ACAGGATGAGCACGTTGAAA+AGG - chr8_1:60761129-60761148 None:intergenic 45.0%
TGCATGGGAAATGTTAACCG+GGG + chr8_1:60761288-60761307 Msa1204910:three_prime_UTR 45.0%
!!! ATGTATAATTTTTTTTTATA+CGG + chr8_1:60759555-60759574 Msa1204910:intron 5.0%
!!! TTTTGTTATATATATATATA+TGG + chr8_1:60761377-60761396 Msa1204910:three_prime_UTR 5.0%
CGACATCACTTGGAATCTCC+TGG - chr8_1:60758067-60758086 None:intergenic 50.0%
GAACTCATCGATGTTCCTGG+TGG + chr8_1:60758474-60758493 Msa1204910:CDS 50.0%
CATCGCTATGCTTCGATGTG+TGG + chr8_1:60758563-60758582 Msa1204910:CDS 50.0%
GCTTCCAAGGAGAGTCAGTT+TGG + chr8_1:60758777-60758796 Msa1204910:CDS 50.0%
ATGTCCAGTCCTGAGTGTGA+GGG - chr8_1:60759138-60759157 None:intergenic 50.0%
AATGTCCAGTCCTGAGTGTG+AGG - chr8_1:60759139-60759158 None:intergenic 50.0%
CACACTCAGGACTGGACATT+TGG + chr8_1:60759139-60759158 Msa1204910:intron 50.0%
!! GTAGCCTGATAGTGGACATC+TGG + chr8_1:60759174-60759193 Msa1204910:intron 50.0%
!! TCCTGTTTTGGGCGTGAATG+GGG - chr8_1:60759292-60759311 None:intergenic 50.0%
!! GTCCTGTTTTGGGCGTGAAT+GGG - chr8_1:60759293-60759312 None:intergenic 50.0%
!! TGTCCTGTTTTGGGCGTGAA+TGG - chr8_1:60759294-60759313 None:intergenic 50.0%
! AGGACATCTAGAGCGTGACA+CGG + chr8_1:60759308-60759327 Msa1204910:intron 50.0%
TCGGTCTTCACCTTACAAGC+TGG - chr8_1:60759439-60759458 None:intergenic 50.0%
CACAGCCTGTCCTAATTGCA+TGG - chr8_1:60759840-60759859 None:intergenic 50.0%
GCAATTAGGACAGGCTGTGT+TGG + chr8_1:60759841-60759860 Msa1204910:three_prime_UTR 50.0%
GATGTGTCGCCGCTAAATGA+AGG - chr8_1:60759881-60759900 None:intergenic 50.0%
!! CTGTCCGTGTCGCTTACAAA+TGG - chr8_1:60760670-60760689 None:intergenic 50.0%
!!! CCTGTTGTTTTGTGCAGCGT+GGG - chr8_1:60760746-60760765 None:intergenic 50.0%
!! TCTTTGGGACGTGCTATGAC+AGG - chr8_1:60761147-60761166 None:intergenic 50.0%
GGGAAATGTTAACCGGGGAA+AGG + chr8_1:60761293-60761312 Msa1204910:three_prime_UTR 50.0%
CTTCCACTGCTTCTCACCAG+AGG + chr8_1:60758847-60758866 Msa1204910:CDS 55.0%
ACCAGAGGCCAGCTGTTGAT+TGG + chr8_1:60758862-60758881 Msa1204910:CDS 55.0%
! ACCAATCAACAGCTGGCCTC+TGG - chr8_1:60758866-60758885 None:intergenic 55.0%
! AGAGGCCAGCTGTTGATTGG+TGG + chr8_1:60758865-60758884 Msa1204910:CDS 55.0%
! GAGGCCAGCTGTTGATTGGT+GGG + chr8_1:60758866-60758885 Msa1204910:CDS 55.0%
TCAGCCCACCAATCAACAGC+TGG - chr8_1:60758873-60758892 None:intergenic 55.0%
TAACACACCCCCTCACACTC+AGG + chr8_1:60759126-60759145 Msa1204910:intron 55.0%
TGTCCAGTCCTGAGTGTGAG+GGG - chr8_1:60759137-60759156 None:intergenic 55.0%
! TTTTGGGCGTGAATGGGGTG+GGG - chr8_1:60759287-60759306 None:intergenic 55.0%
!! GTTTTGGGCGTGAATGGGGT+GGG - chr8_1:60759288-60759307 None:intergenic 55.0%
!! TGTTTTGGGCGTGAATGGGG+TGG - chr8_1:60759289-60759308 None:intergenic 55.0%
ACCCCATTCACGCCCAAAAC+AGG + chr8_1:60759288-60759307 Msa1204910:intron 55.0%
! GGACATCTAGAGCGTGACAC+GGG + chr8_1:60759309-60759328 Msa1204910:intron 55.0%
TCTACCAGGTCACGTGCAAC+TGG - chr8_1:60759338-60759357 None:intergenic 55.0%
GCACGTGACCTGGTAGAAAG+TGG + chr8_1:60759341-60759360 Msa1204910:intron 55.0%
! CTGTTGGGCCACTTTCTACC+AGG - chr8_1:60759352-60759371 None:intergenic 55.0%
CCCACGCTGCACAAAACAAC+AGG + chr8_1:60760743-60760762 Msa1204910:three_prime_UTR 55.0%
!!! GCCTGTTGTTTTGTGCAGCG+TGG - chr8_1:60760747-60760766 None:intergenic 55.0%
CAGGCTTCCTGCCCAAAATG+TGG + chr8_1:60760762-60760781 Msa1204910:three_prime_UTR 55.0%
GCAACGTCTTCGCGACAACA+TGG + chr8_1:60760807-60760826 Msa1204910:three_prime_UTR 55.0%
CGTCTTCGCGACAACATGGA+TGG + chr8_1:60760811-60760830 Msa1204910:three_prime_UTR 55.0%
!! ATGGATGGCACTGGCAGCAT+AGG + chr8_1:60760826-60760845 Msa1204910:three_prime_UTR 55.0%
CGCCTTCAACACCTGACAAG+AGG + chr8_1:60760857-60760876 Msa1204910:three_prime_UTR 55.0%
AACCCTTTGCGGTTGCGGGA+AGG + chr8_1:60757879-60757898 Msa1204910:five_prime_UTR 60.0%
CTCCTTCCCGCAACCGCAAA+GGG - chr8_1:60757884-60757903 None:intergenic 60.0%
TGGCCTCTGGTGAGAAGCAG+TGG - chr8_1:60758853-60758872 None:intergenic 60.0%
GTCCAGTCCTGAGTGTGAGG+GGG - chr8_1:60759136-60759155 None:intergenic 60.0%
TTTGGGCGTGAATGGGGTGG+GGG - chr8_1:60759286-60759305 None:intergenic 60.0%
GTAGCCAGTTGCACGTGACC+TGG + chr8_1:60759331-60759350 Msa1204910:intron 60.0%
GCGCCTCTCGTGATAGCTTC+TGG - chr8_1:60760720-60760739 None:intergenic 60.0%
CGCGACAACATGGATGGCAC+TGG + chr8_1:60760817-60760836 Msa1204910:three_prime_UTR 60.0%
CCTCCTTCCCGCAACCGCAA+AGG - chr8_1:60757885-60757904 None:intergenic 65.0%
CCTTTGCGGTTGCGGGAAGG+AGG + chr8_1:60757882-60757901 Msa1204910:five_prime_UTR 65.0%
CACCCCCTCACACTCAGGAC+TGG + chr8_1:60759131-60759150 Msa1204910:intron 65.0%
TTGGGCGTGAATGGGGTGGG+GGG - chr8_1:60759285-60759304 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 60757747 60761402 60757747 ID=Msa1204910;Name=Msa1204910
chr8_1 mRNA 60757747 60761402 60757747 ID=Msa1204910-mRNA-1;Parent=Msa1204910;Name=Msa1204910-mRNA-1;_AED=0.10;_eAED=0.10;_QI=174|1|1|1|1|0.8|5|1350|210
chr8_1 exon 60757747 60757940 60757747 ID=Msa1204910-mRNA-1:exon:19705;Parent=Msa1204910-mRNA-1
chr8_1 exon 60758055 60758124 60758055 ID=Msa1204910-mRNA-1:exon:19706;Parent=Msa1204910-mRNA-1
chr8_1 exon 60758393 60759018 60758393 ID=Msa1204910-mRNA-1:exon:19707;Parent=Msa1204910-mRNA-1
chr8_1 exon 60759641 60760165 60759641 ID=Msa1204910-mRNA-1:exon:19708;Parent=Msa1204910-mRNA-1
chr8_1 exon 60760661 60761402 60760661 ID=Msa1204910-mRNA-1:exon:19709;Parent=Msa1204910-mRNA-1
chr8_1 five_prime_UTR 60757747 60757920 60757747 ID=Msa1204910-mRNA-1:five_prime_utr;Parent=Msa1204910-mRNA-1
chr8_1 CDS 60757921 60757940 60757921 ID=Msa1204910-mRNA-1:cds;Parent=Msa1204910-mRNA-1
chr8_1 CDS 60758055 60758124 60758055 ID=Msa1204910-mRNA-1:cds;Parent=Msa1204910-mRNA-1
chr8_1 CDS 60758393 60758935 60758393 ID=Msa1204910-mRNA-1:cds;Parent=Msa1204910-mRNA-1
chr8_1 three_prime_UTR 60758936 60759018 60758936 ID=Msa1204910-mRNA-1:three_prime_utr;Parent=Msa1204910-mRNA-1
chr8_1 three_prime_UTR 60759641 60760165 60759641 ID=Msa1204910-mRNA-1:three_prime_utr;Parent=Msa1204910-mRNA-1
chr8_1 three_prime_UTR 60760661 60761402 60760661 ID=Msa1204910-mRNA-1:three_prime_utr;Parent=Msa1204910-mRNA-1
Gene Sequence

>Msa1204910

ATGAAGAGAACAAGTGAATGGAATTACTCCCAGGAGATTCCAAGTGATGTCGCTATTCAAATTGGTGAAGCTTCCTATTCGTTACACAAGTTTCCATTAATTTCCAAAAGTGGACACATAAGGAAGCTCATTTCAGAATCTAGTGATGCTGTTGCTGATGTTCCATTAATTGAACTCATCGATGTTCCTGGTGGATCAGAAGCATTTGAACTAGCAGCAAAGTTCTGTTACGGGATAAACTTCGAAATAAGTGTCGAAAACATCGCTATGCTTCGATGTGTGGCCGAGTATCTTGAAATGACAGAAGATTATTCGGTCGGAAATTTGGTAGGAAGAACTGATTCTTACTTGAATGAAGTTGCACTAAAGAGCATGTCAGGGGCAATATCCATCTTACATACATCAGAAACTCTTCTTCCAATTGCAGAGAAAGCAAAATTAGTTAGCAGATGCATAGATGCAATAGCCTACATAGCTTCCAAGGAGAGTCAGTTTGGTTCATCTGGTAGAAGTGATGGTAGTAGCAATGAAAGAGTGATGAATTCTTCCACTGCTTCTCACCAGAGGCCAGCTGTTGATTGGTGGGCTGAAGATCTAACTGTTCTTAGAATTGACTTTTTCAAAGAGTTCTAA
Protein sequence

>Msa1204910

MKRTSEWNYSQEIPSDVAIQIGEASYSLHKFPLISKSGHIRKLISESSDAVADVPLIELIDVPGGSEAFELAAKFCYGINFEISVENIAMLRCVAEYLEMTEDYSVGNLVGRTDSYLNEVALKSMSGAISILHTSETLLPIAEKAKLVSRCIDAIAYIASKESQFGSSGRSDGSSNERVMNSSTASHQRPAVDWWAEDLTVLRIDFFKEF*