AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1256500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1256500 MtrunA17_Chr4g0053841 97.483 596 14 1 1 596 1 595 0.0 1196
Msa1256500 MtrunA17_Chr5g0401451 70.328 610 160 10 1 596 28 630 0.0 857
Msa1256500 MtrunA17_Chr4g0010091 41.237 582 310 10 2 571 24 585 3.19e-153 454
Msa1256500 MtrunA17_Chr7g0268781 40.095 631 317 18 1 596 25 629 2.93e-140 421
Msa1256500 MtrunA17_Chr1g0195911 39.583 624 322 16 1 596 25 621 1.26e-138 416
Msa1256500 MtrunA17_Chr7g0245201 41.289 574 299 13 12 571 60 609 1.14e-137 415
Msa1256500 MtrunA17_Chr3g0108331 39.439 606 308 11 1 596 12 568 2.71e-136 409
Msa1256500 MtrunA17_Chr5g0432861 37.662 616 315 11 1 596 13 579 5.39e-135 405
Msa1256500 MtrunA17_Chr4g0050021 36.301 584 312 9 2 527 33 614 1.55e-119 369
Msa1256500 MtrunA17_Chr2g0279481 38.826 528 251 8 1 466 32 549 4.45e-117 363
Msa1256500 MtrunA17_Chr2g0303541 34.286 595 346 12 2 566 15 594 4.36e-112 349
Msa1256500 MtrunA17_Chr8g0374011 38.614 505 264 11 7 467 14 516 8.18e-106 333
Msa1256500 MtrunA17_Chr2g0285511 36.179 492 282 9 7 472 20 505 4.73e-105 329
Msa1256500 MtrunA17_Chr8g0382231 36.492 496 246 8 13 470 52 516 1.07e-104 329
Msa1256500 MtrunA17_Chr2g0320691 33.759 548 336 10 6 527 16 562 6.98e-101 319
Msa1256500 MtrunA17_Chr5g0401341 36.887 469 274 7 10 465 24 483 3.19e-97 310
Msa1256500 MtrunA17_Chr1g0150191 37.155 471 271 11 10 463 24 486 4.67e-94 301
Msa1256500 MtrunA17_Chr1g0207461 36.049 491 283 9 10 485 25 499 1.45e-93 300
Msa1256500 MtrunA17_Chr1g0181181 33.947 489 274 8 7 470 20 484 5.65e-92 294
Msa1256500 MtrunA17_Chr4g0076521 36.147 462 280 6 10 465 23 475 5.22e-91 293
Msa1256500 MtrunA17_Chr1g0199631 34.924 461 278 8 31 472 8 465 1.62e-89 288
Msa1256500 MtrunA17_Chr5g0412591 35.050 505 241 10 10 465 23 489 2.04e-83 273
Msa1256500 MtrunA17_Chr1g0206621 89.362 141 15 0 20 160 75 215 6.87e-81 254
Msa1256500 MtrunA17_Chr8g0367891 34.021 485 256 9 16 465 29 484 9.68e-79 260
Msa1256500 MtrunA17_Chr2g0290741 34.279 458 280 9 12 465 25 465 1.63e-78 259
Msa1256500 MtrunA17_Chr1g0191711 29.278 526 323 9 2 518 47 532 3.51e-77 254
Msa1256500 MtrunA17_Chr2g0332281 36.723 354 193 9 195 536 24 358 2.09e-55 193
Msa1256500 MtrunA17_Chr4g0003891 37.574 338 182 8 195 519 46 367 4.73e-55 193
Msa1256500 MtrunA17_Chr4g0023801 26.834 559 353 17 22 554 14 542 9.29e-47 172
Msa1256500 MtrunA17_Chr4g0037661 25.046 539 351 13 16 527 6 518 1.68e-39 152
Msa1256500 MtrunA17_Chr1g0163311 24.819 552 329 20 16 527 6 511 1.22e-33 135
Msa1256500 MtrunA17_Chr2g0280541 26.119 402 252 11 82 467 2 374 8.13e-26 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1256500 AT5G67385.1 63.097 607 198 10 1 596 13 604 0.0 718
Msa1256500 AT5G67385.4 62.369 574 190 10 34 596 1 559 0.0 661
Msa1256500 AT5G67385.5 62.369 574 190 10 34 596 1 559 0.0 661
Msa1256500 AT5G67385.2 62.369 574 190 10 34 596 1 559 0.0 661
Msa1256500 AT5G67385.3 62.369 574 190 10 34 596 1 559 0.0 661
Msa1256500 AT3G49970.1 51.646 577 207 15 25 596 6 515 0.0 531
Msa1256500 AT3G49970.2 51.646 577 207 15 25 596 6 515 0.0 531
Msa1256500 AT5G48800.1 42.759 580 288 9 5 571 34 582 5.53e-155 459
Msa1256500 AT2G30520.1 38.387 620 314 11 1 596 18 593 3.97e-137 412
Msa1256500 AT2G47860.1 42.029 552 279 12 1 527 26 561 5.77e-133 403
Msa1256500 AT1G03010.1 39.370 635 321 18 1 596 25 634 2.81e-132 401
Msa1256500 AT1G03010.2 39.206 630 320 17 6 596 2 607 9.28e-131 396
Msa1256500 AT1G03010.3 39.423 624 315 17 12 596 34 633 3.14e-130 395
Msa1256500 AT3G08570.1 37.902 591 297 12 12 571 33 584 3.54e-128 390
Msa1256500 AT2G47860.3 40.348 575 279 13 1 527 26 584 1.40e-127 390
Msa1256500 AT2G30520.2 38.378 555 291 9 33 574 2 518 3.36e-120 367
Msa1256500 AT2G30520.3 38.378 555 291 9 33 574 2 518 3.36e-120 367
Msa1256500 AT3G08660.1 37.083 569 305 12 12 571 33 557 2.23e-115 355
Msa1256500 AT1G30440.1 34.853 614 337 14 2 566 15 614 1.33e-110 346
Msa1256500 AT5G03250.1 36.447 546 307 11 7 518 20 559 4.54e-108 337
Msa1256500 AT3G08660.3 37.153 541 287 12 40 571 1 497 6.77e-106 329
Msa1256500 AT2G47860.4 43.418 433 211 9 1 414 26 443 2.79e-105 325
Msa1256500 AT5G66560.1 34.006 644 330 18 7 566 13 645 1.04e-101 323
Msa1256500 AT5G13600.1 37.475 491 272 11 7 472 20 500 5.57e-101 318
Msa1256500 AT2G47860.6 41.228 456 211 10 1 414 26 466 3.10e-100 313
Msa1256500 AT5G66560.3 34.185 626 317 18 25 566 2 616 6.84e-97 309
Msa1256500 AT5G66560.4 35.921 554 302 16 55 566 64 606 2.00e-94 303
Msa1256500 AT2G47860.5 39.294 453 240 10 100 527 1 443 1.50e-91 291
Msa1256500 AT2G47860.2 39.294 453 240 10 100 527 1 443 1.50e-91 291
Msa1256500 AT2G14820.2 35.250 539 306 14 12 521 26 550 4.21e-89 289
Msa1256500 AT2G14820.1 35.250 539 306 14 12 521 26 550 4.21e-89 289
Msa1256500 AT4G31820.1 34.118 510 293 11 12 506 26 507 8.09e-89 286
Msa1256500 AT1G67900.5 37.550 498 258 9 10 465 23 509 5.47e-87 283
Msa1256500 AT1G67900.1 37.550 498 258 9 10 465 23 509 5.47e-87 283
Msa1256500 AT1G67900.3 37.550 498 258 9 10 465 23 509 5.47e-87 283
Msa1256500 AT1G67900.6 37.550 498 258 9 10 465 23 509 5.47e-87 283
Msa1256500 AT1G67900.2 37.550 498 258 9 10 465 23 509 5.47e-87 283
Msa1256500 AT1G67900.4 37.550 498 258 9 10 465 23 509 5.47e-87 283
Msa1256500 AT5G67440.3 35.524 487 287 11 10 483 24 496 2.00e-86 280
Msa1256500 AT5G67440.1 35.524 487 287 11 10 483 24 496 2.00e-86 280
Msa1256500 AT3G44820.1 30.159 567 349 11 2 527 12 572 2.04e-85 280
Msa1256500 AT3G50840.1 32.288 542 332 8 2 535 5 519 3.80e-85 276
Msa1256500 AT5G10250.2 33.733 501 266 10 2 467 39 508 2.21e-84 276
Msa1256500 AT4G37590.1 33.915 516 284 12 10 510 24 497 3.49e-83 271
Msa1256500 AT5G10250.1 33.947 489 266 10 2 467 39 493 2.51e-82 270
Msa1256500 AT5G67440.2 35.532 470 276 11 27 483 4 459 1.06e-80 264
Msa1256500 AT3G08660.2 36.562 413 226 8 12 416 33 417 4.02e-79 256
Msa1256500 AT4G31820.2 32.780 482 281 11 40 506 1 454 1.15e-75 250
Msa1256500 AT3G26490.1 32.193 497 284 10 10 465 23 507 1.51e-75 252
Msa1256500 AT3G26490.3 32.193 497 284 10 10 465 23 507 1.51e-75 252
Msa1256500 AT3G26490.4 32.193 497 284 10 10 465 23 507 1.51e-75 252
Msa1256500 AT2G23050.1 32.623 469 277 9 10 474 24 457 3.10e-75 248
Msa1256500 AT3G50840.2 31.961 510 312 8 34 535 1 483 5.86e-74 246
Msa1256500 AT3G50840.3 31.961 510 312 8 34 535 1 483 5.86e-74 246
Msa1256500 AT5G66560.2 34.183 471 243 11 7 414 13 479 1.08e-73 244
Msa1256500 AT5G03250.2 34.437 453 257 11 100 518 1 447 4.31e-73 242
Msa1256500 AT5G47800.3 30.801 487 295 12 12 482 25 485 1.54e-71 240
Msa1256500 AT5G47800.1 30.801 487 295 12 12 482 25 485 1.54e-71 240
Msa1256500 AT5G47800.4 30.801 487 295 12 12 482 25 485 1.54e-71 240
Msa1256500 AT5G47800.2 30.515 485 299 11 12 482 138 598 2.23e-70 240
Msa1256500 AT3G26490.2 30.872 447 256 10 10 415 23 457 3.30e-59 206
Msa1256500 AT5G48130.1 27.680 513 304 13 7 493 33 504 8.04e-54 194
Msa1256500 AT5G64330.2 39.922 258 131 4 3 239 42 296 3.92e-52 190
Msa1256500 AT5G64330.2 37.011 281 142 5 256 503 372 650 4.27e-49 181
Msa1256500 AT5G64330.1 39.922 258 131 4 3 239 42 296 1.35e-51 189
Msa1256500 AT5G64330.1 34.868 304 158 5 256 519 372 675 2.14e-49 183
Msa1256500 AT1G52770.1 33.333 357 200 7 195 522 28 375 1.37e-51 184
Msa1256500 AT5G64330.3 37.011 281 142 5 256 503 313 591 1.55e-49 181
Msa1256500 AT5G64330.3 39.827 231 115 4 30 239 10 237 4.87e-43 163
Msa1256500 AT2G23050.2 30.053 376 227 8 100 474 1 341 3.85e-48 172
Msa1256500 AT3G15570.1 34.266 286 161 5 204 471 38 314 1.30e-43 162
Msa1256500 AT3G19850.1 27.234 470 274 11 33 463 23 463 1.40e-42 161
Msa1256500 AT3G22104.1 27.938 451 278 13 15 452 6 422 2.75e-39 151
Msa1256500 AT1G50280.1 28.172 465 293 11 15 453 5 454 1.07e-38 150
Msa1256500 AT5G17580.2 26.496 468 305 11 15 470 22 462 4.22e-37 145
Msa1256500 AT5G17580.1 25.781 512 337 12 15 510 7 491 6.91e-37 145
Msa1256500 AT1G50280.2 28.878 419 261 9 57 453 18 421 9.24e-37 144
Msa1256500 AT3G03510.1 27.451 408 241 10 69 470 8 366 2.47e-34 136
Msa1256500 AT3G22104.4 26.862 376 233 9 87 452 4 347 2.86e-27 115
Msa1256500 AT3G22104.2 26.862 376 233 9 87 452 4 347 2.86e-27 115
Msa1256500 AT3G22104.3 26.862 376 233 9 87 452 4 347 2.86e-27 115
Msa1256500 AT3G49900.2 30.864 162 100 4 3 159 55 209 1.13e-13 74.3
Msa1256500 AT3G49900.1 30.864 162 100 4 3 159 55 209 1.13e-13 74.3

Find 125 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAATTTGAGCCACATTT+TGG 0.185225 8_2:-56501331 None:intergenic
GTCTAGTTGTATGCGATCTT+TGG 0.210752 8_2:-56501717 None:intergenic
CTTCCTTATGTGTCCACTTT+TGG 0.213031 8_2:-56498962 None:intergenic
CCATTCACTTGTTCTCTTCA+TGG 0.260781 8_2:-56498474 None:intergenic
AGATTATTCGGTCGGAAATT+TGG 0.273291 8_2:+56499164 Msa1256500:CDS
CTGTCAGCTTTCTTTCACTA+TGG 0.279955 8_2:+56500307 Msa1256500:CDS
TGTGAATAAAGCATTATAAT+AGG 0.301041 8_2:-56499534 None:intergenic
GAGTTTAGGAATGACTTTCT+AGG 0.305347 8_2:-56501632 None:intergenic
CTGCAATGCAACCGTGATTA+AGG 0.311893 8_2:-56498397 None:intergenic
TTGAGACAACTGTTGCTTGC+AGG 0.327110 8_2:+56500349 Msa1256500:CDS
TTTGTAAGATATTTATTTGC+AGG 0.329856 8_2:+56501880 Msa1256500:three_prime_UTR
AGTGATGTCACTATTCAAAT+TGG 0.338108 8_2:+56498634 Msa1256500:CDS
TCATTGCAATTAGAACTCTT+TGG 0.356072 8_2:-56499479 None:intergenic
TGTTGCTTGCAGGTTTGATT+TGG 0.361308 8_2:+56500359 Msa1256500:CDS
ATAAGACCCTTTGCGGTTGC+GGG 0.361472 8_2:+56498430 Msa1256500:five_prime_UTR
AAAATATCTTGCACAAAATC+AGG 0.361655 8_2:-56501762 None:intergenic
CATTCTTCTCCCTTGGCATA+AGG 0.363104 8_2:-56500278 None:intergenic
ATTGAACTCATCGATGTTCC+TGG 0.366170 8_2:+56499027 Msa1256500:CDS
TCTTGCACAAAATCAGGATA+TGG 0.373271 8_2:-56501756 None:intergenic
TGAAATGACAGAAGATTATT+CGG 0.380236 8_2:+56499152 Msa1256500:CDS
CTCCTTCCCGCAACCGCAAA+GGG 0.383062 8_2:-56498436 None:intergenic
CTTTCTTTCACTATGGCTTA+AGG 0.386758 8_2:+56500314 Msa1256500:CDS
TCTCTTCATGGCACTAGAAA+TGG 0.387904 8_2:-56498462 None:intergenic
GCGCCTCTCGTGATAGCTTC+TGG 0.389792 8_2:-56501274 None:intergenic
TATGCATGGGAAATGTTAAC+CGG 0.392483 8_2:+56501843 Msa1256500:three_prime_UTR
ACATCGATGAGTTCAATTAA+TGG 0.394415 8_2:-56499021 None:intergenic
AGAAGGATAGCCATGCAATT+AGG 0.395560 8_2:+56500384 Msa1256500:CDS
CATAAGACCCTTTGCGGTTG+CGG 0.402508 8_2:+56498429 Msa1256500:five_prime_UTR
TCTAGTTGTATGCGATCTTT+GGG 0.404869 8_2:-56501716 None:intergenic
TTTAACAAAGGACTATTGAT+AGG 0.405011 8_2:-56501575 None:intergenic
CTAGCAGCAAAGTTCTGTTA+TGG 0.405045 8_2:+56499069 Msa1256500:CDS
TTTCCATTAATTTCCAAAAG+TGG 0.409367 8_2:+56498949 Msa1256500:CDS
GCTTCCAAGGAGAGTCAGTT+TGG 0.413724 8_2:+56499333 Msa1256500:CDS
GTTGCACTAAAGAGCATGTC+AGG 0.420005 8_2:+56499216 Msa1256500:CDS
ACAGAAATCTCTGAGAGGTT+TGG 0.436903 8_2:+56499554 Msa1256500:CDS
TTTCCAAAAGTGGACACATA+AGG 0.437270 8_2:+56498959 Msa1256500:CDS
ACCAATCAACAGCTGGCCTC+TGG 0.440922 8_2:-56499419 None:intergenic
TAGCAGCAAAGTTCTGTTAT+GGG 0.442853 8_2:+56499070 Msa1256500:CDS
TCACGGTTGCATTGCAGTCT+CGG 0.444737 8_2:+56498403 Msa1256500:five_prime_UTR
TTCCTCTTGTCAGGTGTTGA+AGG 0.446976 8_2:-56501416 None:intergenic
AAGAGTTCTAATTGCAATGA+TGG 0.448828 8_2:+56499482 Msa1256500:CDS
ACCAGAGGCCAGCTGTTGAT+TGG 0.449238 8_2:+56499418 Msa1256500:CDS
GAGTATACATTCCTCTTGTC+AGG 0.452843 8_2:-56501425 None:intergenic
AGCCGTAGACATCTATCTTA+AGG 0.453245 8_2:+56500701 Msa1256500:CDS
GAGAGTCAGTTTGGTTCGTC+TGG 0.454571 8_2:+56499342 Msa1256500:CDS
AACTACCTTGTGTAACGAAT+AGG 0.461457 8_2:-56498665 None:intergenic
GATATTGCATTCTTCTCCCT+TGG 0.461512 8_2:-56500285 None:intergenic
AGAGGCCAGCTGTTGATTGG+TGG 0.462714 8_2:+56499421 Msa1256500:CDS
CCCACGCTGCACAAAACAAC+AGG 0.463975 8_2:+56501300 Msa1256500:CDS
CTGTCCGTGTCGCTTACAAA+TGG 0.464751 8_2:-56501224 None:intergenic
TGACATCACTTGGAATCTCC+TGG 0.465336 8_2:-56498621 None:intergenic
CGAACCAAACTGACTCTCCT+TGG 0.467724 8_2:-56499337 None:intergenic
CGGATACATAAGACCCTTTG+CGG 0.472650 8_2:+56498423 Msa1256500:five_prime_UTR
AACTCAGTTTCAAGAAAACT+TGG 0.478198 8_2:+56501650 Msa1256500:CDS
CCTCCTTCCCGCAACCGCAA+AGG 0.478959 8_2:-56498437 None:intergenic
AAATCAAGAACAACAGAAGA+TGG 0.483167 8_2:+56500669 Msa1256500:CDS
AAAGAAAGTTTGCAGCATGA+TGG 0.486600 8_2:+56501247 Msa1256500:CDS
ACTCTCCTTGGAAGCTATGT+AGG 0.486973 8_2:-56499325 None:intergenic
TGATTAAGGCTGTATTGTAT+TGG 0.494203 8_2:-56498383 None:intergenic
ACAGGATGAGCACGTTGAAA+AGG 0.498236 8_2:-56501683 None:intergenic
GATGTGTCGCCGCTAAATGA+AGG 0.498482 8_2:-56500435 None:intergenic
GTATTATTCTTCTCCACAAA+GGG 0.500177 8_2:+56500539 Msa1256500:CDS
AGGAATGACTTTCTAGGCAA+AGG 0.500562 8_2:-56501626 None:intergenic
TTGGAAACCTCATTAGAAGC+TGG 0.504132 8_2:-56501461 None:intergenic
TTCTGTCATTTCAAGATACT+CGG 0.511486 8_2:-56499142 None:intergenic
CTCTTCTCATGCTCTTCATG+TGG 0.516105 8_2:-56500231 None:intergenic
GGTTCGTCTGGTCGAAGTGA+TGG 0.519063 8_2:+56499354 Msa1256500:CDS
GATATTTGGAAAGGGAAGGA+AGG 0.520414 8_2:+56500200 Msa1256500:CDS
GAGCAGGAGATATTTGGAAA+GGG 0.520665 8_2:+56500192 Msa1256500:intron
AGTTTCTGATGTATGTAAGA+TGG 0.522762 8_2:-56499247 None:intergenic
GCTTTATCCAGCTTCTAATG+AGG 0.525232 8_2:+56501454 Msa1256500:CDS
GAGGCCAGCTGTTGATTGGT+GGG 0.529140 8_2:+56499422 Msa1256500:CDS
ATGGATGGCACTGGCAGCAT+AGG 0.533950 8_2:+56501383 Msa1256500:CDS
CATCGCTATGCTTCGATGTG+TGG 0.533993 8_2:+56499119 Msa1256500:CDS
TTGTAAGATATTTATTTGCA+GGG 0.534022 8_2:+56501881 Msa1256500:three_prime_UTR
ATTTGAATAGTGACATCACT+TGG 0.537748 8_2:-56498631 None:intergenic
GACCCTTTGCGGTTGCGGGA+AGG 0.540883 8_2:+56498434 Msa1256500:five_prime_UTR
TCTTTGGGACGTGCTATGAC+AGG 0.544164 8_2:-56501701 None:intergenic
TCAGCCCACCAATCAACAGC+TGG 0.545487 8_2:-56499426 None:intergenic
GGAGCAGGAGATATTTGGAA+AGG 0.547365 8_2:+56500191 Msa1256500:intron
TATTCGGTCGGAAATTTGGT+AGG 0.547468 8_2:+56499168 Msa1256500:CDS
CGCCTTCAACACCTGACAAG+AGG 0.552605 8_2:+56501414 Msa1256500:CDS
TTATCACAGGAATTACTCCC+AGG 0.555974 8_2:+56498603 Msa1256500:intron
GATAGCCATGCAATTAGGAC+AGG 0.556430 8_2:+56500389 Msa1256500:CDS
AGGAGATATTTGGAAAGGGA+AGG 0.557338 8_2:+56500196 Msa1256500:intron
AATAGCCTACATAGCTTCCA+AGG 0.561754 8_2:+56499320 Msa1256500:CDS
CCATGAAGAGAACAAGTGAA+TGG 0.562517 8_2:+56498474 Msa1256500:exon
GCAATTAGGACAGGCTGTGT+TGG 0.562742 8_2:+56500398 Msa1256500:CDS
GCAGGTTTGATTTGGAGAGA+AGG 0.563785 8_2:+56500367 Msa1256500:CDS
GCAACGTCTTCGCGACAACA+TGG 0.564907 8_2:+56501364 Msa1256500:CDS
TACTAGTGAGCCTTATGCCA+AGG 0.566316 8_2:+56500268 Msa1256500:CDS
CCTTTGCGGTTGCGGGAAGG+AGG 0.570427 8_2:+56498437 Msa1256500:five_prime_UTR
CACAGCCTGTCCTAATTGCA+TGG 0.571835 8_2:-56500394 None:intergenic
CGCGACAACATGGATGGCAC+TGG 0.572937 8_2:+56501374 Msa1256500:CDS
AGTATTATTCTTCTCCACAA+AGG 0.574490 8_2:+56500538 Msa1256500:CDS
GACATCACTTGGAATCTCCT+GGG 0.574678 8_2:-56498620 None:intergenic
CTAATTGCAATGATGGCTAG+AGG 0.579105 8_2:+56499489 Msa1256500:CDS
ATGACAGAAGATTATTCGGT+CGG 0.582614 8_2:+56499156 Msa1256500:CDS
ATGCATGGGAAATGTTAACC+GGG 0.582676 8_2:+56501844 Msa1256500:three_prime_UTR
TGGCCTCTGGTGAGAAGCAG+TGG 0.585433 8_2:-56499406 None:intergenic
ATTAGAAGCTGGATAAAGCT+CGG 0.591749 8_2:-56501450 None:intergenic
CAGGCTTCCTGCCCAAAATG+TGG 0.597481 8_2:+56501319 Msa1256500:CDS
GGGAAATGTTAACCGGGGAA+AGG 0.597771 8_2:+56501850 Msa1256500:three_prime_UTR
TAATTGCAATGATGGCTAGA+GGG 0.601649 8_2:+56499490 Msa1256500:CDS
AAAGGAGGCTTATCAGCTGA+AGG 0.602067 8_2:-56501608 None:intergenic
CGTCTTCGCGACAACATGGA+TGG 0.604136 8_2:+56501368 Msa1256500:CDS
TACCTTAAGATAGATGTCTA+CGG 0.604585 8_2:-56500703 None:intergenic
CTTCCACTGCTTCTCACCAG+AGG 0.605670 8_2:+56499403 Msa1256500:CDS
GTTGTGCGTGATATTATGCA+TGG 0.609354 8_2:+56501829 Msa1256500:three_prime_UTR
CACGTTGAAAAGGAGAAAGA+CGG 0.618103 8_2:-56501673 None:intergenic
GGTGATGATGCATTTAACAA+AGG 0.620143 8_2:-56501587 None:intergenic
AGCTTCCTATTCGTTACACA+AGG 0.621103 8_2:+56498660 Msa1256500:CDS
ACTAGTGAGCCTTATGCCAA+GGG 0.621863 8_2:+56500269 Msa1256500:CDS
GAACTCATCGATGTTCCTGG+TGG 0.647010 8_2:+56499030 Msa1256500:CDS
TTGCACTAAAGAGCATGTCA+GGG 0.650226 8_2:+56499217 Msa1256500:CDS
TATCAGTAGCTATTTCAGCA+AGG 0.650241 8_2:-56500596 None:intergenic
AATGACTTTCTAGGCAAAGG+AGG 0.650612 8_2:-56501623 None:intergenic
TCAAATGCTTCTGATCCACC+AGG 0.656366 8_2:-56499045 None:intergenic
TGCACTAAAGAGCATGTCAG+GGG 0.667865 8_2:+56499218 Msa1256500:CDS
TTGTGCGTGATATTATGCAT+GGG 0.675438 8_2:+56501830 Msa1256500:three_prime_UTR
TTTCCAGAAGCTATCACGAG+AGG 0.681776 8_2:+56501271 Msa1256500:CDS
TATTCACAGAAATCTCTGAG+AGG 0.685412 8_2:+56499549 Msa1256500:CDS
ATACAATACAGCCTTAATCA+CGG 0.691333 8_2:+56498386 Msa1256500:five_prime_UTR
TGCATGGGAAATGTTAACCG+GGG 0.704804 8_2:+56501845 Msa1256500:three_prime_UTR
ACATCCATTTGTAAGCGACA+CGG 0.708353 8_2:+56501220 Msa1256500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTATCAAGAA+TGG + chr8_2:56498837-56498856 Msa1256500:intron 10.0%
!! TAAAATAAAAATAAAAGAGA+CGG + chr8_2:56501529-56501548 Msa1256500:CDS 10.0%
!! TAAATATAAGTTTATAAGTG+AGG - chr8_2:56500019-56500038 None:intergenic 15.0%
!! TCACTTATAAACTTATATTT+AGG + chr8_2:56500018-56500037 Msa1256500:intron 15.0%
!!! TTATATTTTATCTAAACTAG+TGG - chr8_2:56500084-56500103 None:intergenic 15.0%
!!! CAAGATATTTTTATCTTTTT+TGG + chr8_2:56501774-56501793 Msa1256500:three_prime_UTR 15.0%
!! GAAAAAGTTAATATTAGCAT+CGG - chr8_2:56498540-56498559 None:intergenic 20.0%
!!! TGCTAATATTAACTTTTTCT+TGG + chr8_2:56498541-56498560 Msa1256500:intron 20.0%
!!! TTTTTATCAAGAATGGTAAT+AGG + chr8_2:56498844-56498863 Msa1256500:intron 20.0%
!!! TGTGAATAAAGCATTATAAT+AGG - chr8_2:56499537-56499556 None:intergenic 20.0%
!!! AATTTTCCTTCTTTAGTAAA+GGG - chr8_2:56499585-56499604 None:intergenic 20.0%
!!! AAATTTTCCTTCTTTAGTAA+AGG - chr8_2:56499586-56499605 None:intergenic 20.0%
!!! TTTCATATCAGTTTTGTTTT+AGG + chr8_2:56499604-56499623 Msa1256500:intron 20.0%
!!! AAAATTTTAAGATGGTATCA+GGG - chr8_2:56499944-56499963 None:intergenic 20.0%
!! AATTCTAACTTTATTAGCAT+TGG - chr8_2:56501186-56501205 None:intergenic 20.0%
!!! TTCATTTTCTCTTCTTAATT+TGG - chr8_2:56501483-56501502 None:intergenic 20.0%
!! TTTGTAAGATATTTATTTGC+AGG + chr8_2:56501880-56501899 Msa1256500:three_prime_UTR 20.0%
!! TTGTAAGATATTTATTTGCA+GGG + chr8_2:56501881-56501900 Msa1256500:three_prime_UTR 20.0%
! TGATAAGTAGTTCAATTCAA+AGG - chr8_2:56498589-56498608 None:intergenic 25.0%
! ACAAACTCAAATATCAAACT+TGG + chr8_2:56498689-56498708 Msa1256500:intron 25.0%
! TTTCCATTAATTTCCAAAAG+TGG + chr8_2:56498949-56498968 Msa1256500:CDS 25.0%
! TGAAATGACAGAAGATTATT+CGG + chr8_2:56499152-56499171 Msa1256500:CDS 25.0%
! TGAAAATACCTTTATAAGGT+AGG - chr8_2:56499777-56499796 None:intergenic 25.0%
!!! TACCTTATAAAGGTATTTTC+AGG + chr8_2:56499776-56499795 Msa1256500:intron 25.0%
!!! CAAAATTTTAAGATGGTATC+AGG - chr8_2:56499945-56499964 None:intergenic 25.0%
!! CTGATACCATCTTAAAATTT+TGG + chr8_2:56499943-56499962 Msa1256500:intron 25.0%
!! ACCATCTTAAAATTTTGGAT+TGG + chr8_2:56499948-56499967 Msa1256500:intron 25.0%
!! CCATCTTAAAATTTTGGATT+GGG + chr8_2:56499949-56499968 Msa1256500:intron 25.0%
! TATAAGTTTATAAGTGAGGT+CGG - chr8_2:56500015-56500034 None:intergenic 25.0%
! AAGATTTGATTAGATGTATG+TGG - chr8_2:56500044-56500063 None:intergenic 25.0%
!!! ATTGAACCTTGACTATTTTT+TGG + chr8_2:56500170-56500189 Msa1256500:intron 25.0%
! ATTTACGACAACAATTTAGA+TGG + chr8_2:56500789-56500808 Msa1256500:intron 25.0%
! AAAATTGTGGTTAAGTATGA+TGG - chr8_2:56500891-56500910 None:intergenic 25.0%
! CAATTTGTGTTCAAAAATTG+TGG - chr8_2:56500904-56500923 None:intergenic 25.0%
!! CAATTTTTGAACACAAATTG+TGG + chr8_2:56500904-56500923 Msa1256500:intron 25.0%
! AGTATTATCCTTGAAATCAA+AGG + chr8_2:56501115-56501134 Msa1256500:intron 25.0%
! AGTTAGAATTCAAAATTCTC+AGG + chr8_2:56501196-56501215 Msa1256500:intron 25.0%
! TTTAACAAAGGACTATTGAT+AGG - chr8_2:56501578-56501597 None:intergenic 25.0%
! AAAATATCTTGCACAAAATC+AGG - chr8_2:56501765-56501784 None:intergenic 25.0%
! TGCTTTTATGAGAGTTTGAA+TGG - chr8_2:56498310-56498329 None:intergenic 30.0%
! TATATGTATTTTGTGCTGAG+TGG - chr8_2:56498337-56498356 None:intergenic 30.0%
TGATTAAGGCTGTATTGTAT+TGG - chr8_2:56498386-56498405 None:intergenic 30.0%
ATACAATACAGCCTTAATCA+CGG + chr8_2:56498386-56498405 Msa1256500:five_prime_UTR 30.0%
TGAATTGAACTACTTATCAC+AGG + chr8_2:56498590-56498609 Msa1256500:intron 30.0%
ATTTGAATAGTGACATCACT+TGG - chr8_2:56498634-56498653 None:intergenic 30.0%
AGTGATGTCACTATTCAAAT+TGG + chr8_2:56498634-56498653 Msa1256500:CDS 30.0%
TGGTTATGAATTCTAGTACT+TGG + chr8_2:56498709-56498728 Msa1256500:intron 30.0%
!! TGTCCACTTTTGGAAATTAA+TGG - chr8_2:56498955-56498974 None:intergenic 30.0%
ACATCGATGAGTTCAATTAA+TGG - chr8_2:56499024-56499043 None:intergenic 30.0%
! TTCTGTCATTTCAAGATACT+CGG - chr8_2:56499145-56499164 None:intergenic 30.0%
AGTTTCTGATGTATGTAAGA+TGG - chr8_2:56499250-56499269 None:intergenic 30.0%
! AATTTTGCTTTCTCTGCAAT+TGG - chr8_2:56499279-56499298 None:intergenic 30.0%
TCATTGCAATTAGAACTCTT+TGG - chr8_2:56499482-56499501 None:intergenic 30.0%
! AAGAGTTCTAATTGCAATGA+TGG + chr8_2:56499482-56499501 Msa1256500:CDS 30.0%
GGGTTTAAAAATTGTGGTAT+TGG + chr8_2:56499510-56499529 Msa1256500:CDS 30.0%
GACCTGAAAATACCTTTATA+AGG - chr8_2:56499781-56499800 None:intergenic 30.0%
!! CCCAATCCAAAATTTTAAGA+TGG - chr8_2:56499952-56499971 None:intergenic 30.0%
! CTTCTTTTTCCTTCATTTAG+CGG + chr8_2:56500426-56500445 Msa1256500:CDS 30.0%
! GATTTTCAACTTCAGATTCA+AGG - chr8_2:56500500-56500519 None:intergenic 30.0%
AGTATTATTCTTCTCCACAA+AGG + chr8_2:56500538-56500557 Msa1256500:CDS 30.0%
GTATTATTCTTCTCCACAAA+GGG + chr8_2:56500539-56500558 Msa1256500:CDS 30.0%
! TCTGTTGTTCTTGATTTTTC+AGG - chr8_2:56500666-56500685 None:intergenic 30.0%
AAATCAAGAACAACAGAAGA+TGG + chr8_2:56500669-56500688 Msa1256500:CDS 30.0%
TACCTTAAGATAGATGTCTA+CGG - chr8_2:56500706-56500725 None:intergenic 30.0%
GACGATTATTGAGTATAACA+AGG + chr8_2:56500850-56500869 Msa1256500:intron 30.0%
GTAAGTTCAATTCTGTTATG+TGG - chr8_2:56501092-56501111 None:intergenic 30.0%
! AGTTAAGTCCTTTGATTTCA+AGG - chr8_2:56501126-56501145 None:intergenic 30.0%
! GTTTTCTTGAAACTGAGTTT+AGG - chr8_2:56501649-56501668 None:intergenic 30.0%
AACTCAGTTTCAAGAAAACT+TGG + chr8_2:56501650-56501669 Msa1256500:CDS 30.0%
!!! GTTTTGTTTTGTTCACTTAG+TGG + chr8_2:56501909-56501928 Msa1256500:three_prime_UTR 30.0%
AACTACCTTGTGTAACGAAT+AGG - chr8_2:56498668-56498687 None:intergenic 35.0%
ACTAAGAAACAACCAATACG+CGG - chr8_2:56498810-56498829 None:intergenic 35.0%
TTTCCAAAAGTGGACACATA+AGG + chr8_2:56498959-56498978 Msa1256500:CDS 35.0%
TAGCAGCAAAGTTCTGTTAT+GGG + chr8_2:56499070-56499089 Msa1256500:CDS 35.0%
ATGACAGAAGATTATTCGGT+CGG + chr8_2:56499156-56499175 Msa1256500:CDS 35.0%
AGATTATTCGGTCGGAAATT+TGG + chr8_2:56499164-56499183 Msa1256500:CDS 35.0%
TAATTGCAATGATGGCTAGA+GGG + chr8_2:56499490-56499509 Msa1256500:CDS 35.0%
GCTAGAGGGTTTAAAAATTG+TGG + chr8_2:56499504-56499523 Msa1256500:CDS 35.0%
TATTCACAGAAATCTCTGAG+AGG + chr8_2:56499549-56499568 Msa1256500:CDS 35.0%
GTAAGTCCCTTTACTAAAGA+AGG + chr8_2:56499576-56499595 Msa1256500:intron 35.0%
CCTTTATAAGGTAGGTAGTT+CGG - chr8_2:56499769-56499788 None:intergenic 35.0%
!!! TTTTTGGAGCAGGAGATATT+TGG + chr8_2:56500186-56500205 Msa1256500:intron 35.0%
CTTTCTTTCACTATGGCTTA+AGG + chr8_2:56500314-56500333 Msa1256500:CDS 35.0%
! CTCCACAAAGGGATATTTTT+CGG + chr8_2:56500550-56500569 Msa1256500:CDS 35.0%
! TCCACAAAGGGATATTTTTC+GGG + chr8_2:56500551-56500570 Msa1256500:CDS 35.0%
!!! TTTTCGGGTTGGAAAGTTAT+TGG + chr8_2:56500566-56500585 Msa1256500:CDS 35.0%
! TATCAGTAGCTATTTCAGCA+AGG - chr8_2:56500599-56500618 None:intergenic 35.0%
GCTAAAGCAGTTCAAATATG+TGG + chr8_2:56500811-56500830 Msa1256500:intron 35.0%
AATCTGTGTTCGCATATAGA+TGG - chr8_2:56500958-56500977 None:intergenic 35.0%
ATATGCGAACACAGATTAGA+TGG + chr8_2:56500961-56500980 Msa1256500:intron 35.0%
! CGTCGTTAACTATGATTTTC+AGG - chr8_2:56501019-56501038 None:intergenic 35.0%
GTTAACGACGTTCAAAATCA+AGG + chr8_2:56501029-56501048 Msa1256500:intron 35.0%
AAAGAAAGTTTGCAGCATGA+TGG + chr8_2:56501247-56501266 Msa1256500:CDS 35.0%
! GAGAATTTGAGCCACATTTT+GGG - chr8_2:56501333-56501352 None:intergenic 35.0%
! AGAGAATTTGAGCCACATTT+TGG - chr8_2:56501334-56501353 None:intergenic 35.0%
ATTAGAAGCTGGATAAAGCT+CGG - chr8_2:56501453-56501472 None:intergenic 35.0%
! GGTGATGATGCATTTAACAA+AGG - chr8_2:56501590-56501609 None:intergenic 35.0%
GAGTTTAGGAATGACTTTCT+AGG - chr8_2:56501635-56501654 None:intergenic 35.0%
TCTAGTTGTATGCGATCTTT+GGG - chr8_2:56501719-56501738 None:intergenic 35.0%
TCTTGCACAAAATCAGGATA+TGG - chr8_2:56501759-56501778 None:intergenic 35.0%
TTGTGCGTGATATTATGCAT+GGG + chr8_2:56501830-56501849 Msa1256500:three_prime_UTR 35.0%
TATGCATGGGAAATGTTAAC+CGG + chr8_2:56501843-56501862 Msa1256500:three_prime_UTR 35.0%
! ACAAATGTTTTACCTTTCCC+CGG - chr8_2:56501865-56501884 None:intergenic 35.0%
TCTCTTCATGGCACTAGAAA+TGG - chr8_2:56498465-56498484 None:intergenic 40.0%
CCATTCACTTGTTCTCTTCA+TGG - chr8_2:56498477-56498496 None:intergenic 40.0%
CCATGAAGAGAACAAGTGAA+TGG + chr8_2:56498474-56498493 Msa1256500:exon 40.0%
ACAAGTGAATGGTATGCTTC+TGG + chr8_2:56498485-56498504 Msa1256500:intron 40.0%
TTATCACAGGAATTACTCCC+AGG + chr8_2:56498603-56498622 Msa1256500:intron 40.0%
AGCTTCCTATTCGTTACACA+AGG + chr8_2:56498660-56498679 Msa1256500:CDS 40.0%
! ATTTAGTTGACTCCGCGTAT+TGG + chr8_2:56498795-56498814 Msa1256500:intron 40.0%
! CTTCCTTATGTGTCCACTTT+TGG - chr8_2:56498965-56498984 None:intergenic 40.0%
ATTGAACTCATCGATGTTCC+TGG + chr8_2:56499027-56499046 Msa1256500:CDS 40.0%
CTAGCAGCAAAGTTCTGTTA+TGG + chr8_2:56499069-56499088 Msa1256500:CDS 40.0%
TATTCGGTCGGAAATTTGGT+AGG + chr8_2:56499168-56499187 Msa1256500:CDS 40.0%
! TTGCACTAAAGAGCATGTCA+GGG + chr8_2:56499217-56499236 Msa1256500:CDS 40.0%
AATAGCCTACATAGCTTCCA+AGG + chr8_2:56499320-56499339 Msa1256500:CDS 40.0%
CTAATTGCAATGATGGCTAG+AGG + chr8_2:56499489-56499508 Msa1256500:CDS 40.0%
! ACAGAAATCTCTGAGAGGTT+TGG + chr8_2:56499554-56499573 Msa1256500:CDS 40.0%
TAAGATGAGTAGCCTGATAG+TGG + chr8_2:56499723-56499742 Msa1256500:intron 40.0%
AAATCCAGATGTCCACTATC+AGG - chr8_2:56499738-56499757 None:intergenic 40.0%
ATAAGGTAGGTAGTTCGGTA+AGG - chr8_2:56499764-56499783 None:intergenic 40.0%
CCGAACTACCTACCTTATAA+AGG + chr8_2:56499766-56499785 Msa1256500:intron 40.0%
GTCTGTTAAGAGTCTCACAT+TGG - chr8_2:56499813-56499832 None:intergenic 40.0%
GGGTCTATCCAATATCTGTT+GGG - chr8_2:56499924-56499943 None:intergenic 40.0%
AGGGTCTATCCAATATCTGT+TGG - chr8_2:56499925-56499944 None:intergenic 40.0%
TCTTACAGAACCAGCTTGTA+AGG + chr8_2:56499983-56500002 Msa1256500:intron 40.0%
CCTGCTCCAAAAAATAGTCA+AGG - chr8_2:56500179-56500198 None:intergenic 40.0%
!!! CCTTGACTATTTTTTGGAGC+AGG + chr8_2:56500176-56500195 Msa1256500:intron 40.0%
GAGCAGGAGATATTTGGAAA+GGG + chr8_2:56500192-56500211 Msa1256500:intron 40.0%
AGGAGATATTTGGAAAGGGA+AGG + chr8_2:56500196-56500215 Msa1256500:intron 40.0%
GATATTTGGAAAGGGAAGGA+AGG + chr8_2:56500200-56500219 Msa1256500:CDS 40.0%
GATATTGCATTCTTCTCCCT+TGG - chr8_2:56500288-56500307 None:intergenic 40.0%
CTGTCAGCTTTCTTTCACTA+TGG + chr8_2:56500307-56500326 Msa1256500:CDS 40.0%
! TGTTGCTTGCAGGTTTGATT+TGG + chr8_2:56500359-56500378 Msa1256500:CDS 40.0%
AGAAGGATAGCCATGCAATT+AGG + chr8_2:56500384-56500403 Msa1256500:CDS 40.0%
ACCCGAAAAATATCCCTTTG+TGG - chr8_2:56500555-56500574 None:intergenic 40.0%
!!! CAAAGGGATATTTTTCGGGT+TGG + chr8_2:56500555-56500574 Msa1256500:CDS 40.0%
AGCCGTAGACATCTATCTTA+AGG + chr8_2:56500701-56500720 Msa1256500:CDS 40.0%
ACATCCATTTGTAAGCGACA+CGG + chr8_2:56501220-56501239 Msa1256500:CDS 40.0%
GAGTATACATTCCTCTTGTC+AGG - chr8_2:56501428-56501447 None:intergenic 40.0%
GCTTTATCCAGCTTCTAATG+AGG + chr8_2:56501454-56501473 Msa1256500:CDS 40.0%
TTGGAAACCTCATTAGAAGC+TGG - chr8_2:56501464-56501483 None:intergenic 40.0%
AATGACTTTCTAGGCAAAGG+AGG - chr8_2:56501626-56501645 None:intergenic 40.0%
AGGAATGACTTTCTAGGCAA+AGG - chr8_2:56501629-56501648 None:intergenic 40.0%
CACGTTGAAAAGGAGAAAGA+CGG - chr8_2:56501676-56501695 None:intergenic 40.0%
GTCTAGTTGTATGCGATCTT+TGG - chr8_2:56501720-56501739 None:intergenic 40.0%
GTTGTGCGTGATATTATGCA+TGG + chr8_2:56501829-56501848 Msa1256500:three_prime_UTR 40.0%
ATGCATGGGAAATGTTAACC+GGG + chr8_2:56501844-56501863 Msa1256500:three_prime_UTR 40.0%
CTGCAATGCAACCGTGATTA+AGG - chr8_2:56498400-56498419 None:intergenic 45.0%
CGGATACATAAGACCCTTTG+CGG + chr8_2:56498423-56498442 Msa1256500:five_prime_UTR 45.0%
GACATCACTTGGAATCTCCT+GGG - chr8_2:56498623-56498642 None:intergenic 45.0%
TGACATCACTTGGAATCTCC+TGG - chr8_2:56498624-56498643 None:intergenic 45.0%
TCAAATGCTTCTGATCCACC+AGG - chr8_2:56499048-56499067 None:intergenic 45.0%
! GTTGCACTAAAGAGCATGTC+AGG + chr8_2:56499216-56499235 Msa1256500:CDS 45.0%
! TGCACTAAAGAGCATGTCAG+GGG + chr8_2:56499218-56499237 Msa1256500:CDS 45.0%
ACTCTCCTTGGAAGCTATGT+AGG - chr8_2:56499328-56499347 None:intergenic 45.0%
!! CGCTCTAGATGTCCTGTTTT+GGG - chr8_2:56499860-56499879 None:intergenic 45.0%
!! ACGCTCTAGATGTCCTGTTT+TGG - chr8_2:56499861-56499880 None:intergenic 45.0%
GAAAGTGGCCCAACAGATAT+TGG + chr8_2:56499913-56499932 Msa1256500:intron 45.0%
GGAGCAGGAGATATTTGGAA+AGG + chr8_2:56500191-56500210 Msa1256500:intron 45.0%
CTCTTCTCATGCTCTTCATG+TGG - chr8_2:56500234-56500253 None:intergenic 45.0%
! TACTAGTGAGCCTTATGCCA+AGG + chr8_2:56500268-56500287 Msa1256500:CDS 45.0%
! ACTAGTGAGCCTTATGCCAA+GGG + chr8_2:56500269-56500288 Msa1256500:CDS 45.0%
CATTCTTCTCCCTTGGCATA+AGG - chr8_2:56500281-56500300 None:intergenic 45.0%
! TTGAGACAACTGTTGCTTGC+AGG + chr8_2:56500349-56500368 Msa1256500:CDS 45.0%
!! GCAGGTTTGATTTGGAGAGA+AGG + chr8_2:56500367-56500386 Msa1256500:CDS 45.0%
GATAGCCATGCAATTAGGAC+AGG + chr8_2:56500389-56500408 Msa1256500:CDS 45.0%
TTTCCAGAAGCTATCACGAG+AGG + chr8_2:56501271-56501290 Msa1256500:CDS 45.0%
! ATTTGAGCCACATTTTGGGC+AGG - chr8_2:56501329-56501348 None:intergenic 45.0%
!! TTCCTCTTGTCAGGTGTTGA+AGG - chr8_2:56501419-56501438 None:intergenic 45.0%
AAAGGAGGCTTATCAGCTGA+AGG - chr8_2:56501611-56501630 None:intergenic 45.0%
ACAGGATGAGCACGTTGAAA+AGG - chr8_2:56501686-56501705 None:intergenic 45.0%
TGCATGGGAAATGTTAACCG+GGG + chr8_2:56501845-56501864 Msa1256500:three_prime_UTR 45.0%
!!! ATGTATAATTTTTTTTTATA+CGG + chr8_2:56500112-56500131 Msa1256500:intron 5.0%
!!! TTTTGTTATATATATATATA+TGG + chr8_2:56501934-56501953 Msa1256500:three_prime_UTR 5.0%
TCACGGTTGCATTGCAGTCT+CGG + chr8_2:56498403-56498422 Msa1256500:five_prime_UTR 50.0%
CATAAGACCCTTTGCGGTTG+CGG + chr8_2:56498429-56498448 Msa1256500:five_prime_UTR 50.0%
ATAAGACCCTTTGCGGTTGC+GGG + chr8_2:56498430-56498449 Msa1256500:five_prime_UTR 50.0%
GAACTCATCGATGTTCCTGG+TGG + chr8_2:56499030-56499049 Msa1256500:CDS 50.0%
CATCGCTATGCTTCGATGTG+TGG + chr8_2:56499119-56499138 Msa1256500:CDS 50.0%
GCTTCCAAGGAGAGTCAGTT+TGG + chr8_2:56499333-56499352 Msa1256500:CDS 50.0%
! CGAACCAAACTGACTCTCCT+TGG - chr8_2:56499340-56499359 None:intergenic 50.0%
! GAGAGTCAGTTTGGTTCGTC+TGG + chr8_2:56499342-56499361 Msa1256500:CDS 50.0%
ATGTCCAGTCCTGAGTGTGA+GGG - chr8_2:56499695-56499714 None:intergenic 50.0%
AATGTCCAGTCCTGAGTGTG+AGG - chr8_2:56499696-56499715 None:intergenic 50.0%
CACACTCAGGACTGGACATT+TGG + chr8_2:56499696-56499715 Msa1256500:intron 50.0%
!! GTAGCCTGATAGTGGACATC+TGG + chr8_2:56499731-56499750 Msa1256500:intron 50.0%
!! TCCTGTTTTGGGCGTGAATG+GGG - chr8_2:56499849-56499868 None:intergenic 50.0%
!! GTCCTGTTTTGGGCGTGAAT+GGG - chr8_2:56499850-56499869 None:intergenic 50.0%
!! TGTCCTGTTTTGGGCGTGAA+TGG - chr8_2:56499851-56499870 None:intergenic 50.0%
! AGGACATCTAGAGCGTGACA+CGG + chr8_2:56499865-56499884 Msa1256500:intron 50.0%
TCGGTCTTCACCTTACAAGC+TGG - chr8_2:56499996-56500015 None:intergenic 50.0%
CACAGCCTGTCCTAATTGCA+TGG - chr8_2:56500397-56500416 None:intergenic 50.0%
GCAATTAGGACAGGCTGTGT+TGG + chr8_2:56500398-56500417 Msa1256500:CDS 50.0%
GATGTGTCGCCGCTAAATGA+AGG - chr8_2:56500438-56500457 None:intergenic 50.0%
!! CTGTCCGTGTCGCTTACAAA+TGG - chr8_2:56501227-56501246 None:intergenic 50.0%
!!! CCTGTTGTTTTGTGCAGCGT+GGG - chr8_2:56501303-56501322 None:intergenic 50.0%
!! TCTTTGGGACGTGCTATGAC+AGG - chr8_2:56501704-56501723 None:intergenic 50.0%
GGGAAATGTTAACCGGGGAA+AGG + chr8_2:56501850-56501869 Msa1256500:three_prime_UTR 50.0%
GGTTCGTCTGGTCGAAGTGA+TGG + chr8_2:56499354-56499373 Msa1256500:CDS 55.0%
CTTCCACTGCTTCTCACCAG+AGG + chr8_2:56499403-56499422 Msa1256500:CDS 55.0%
ACCAGAGGCCAGCTGTTGAT+TGG + chr8_2:56499418-56499437 Msa1256500:CDS 55.0%
! ACCAATCAACAGCTGGCCTC+TGG - chr8_2:56499422-56499441 None:intergenic 55.0%
! AGAGGCCAGCTGTTGATTGG+TGG + chr8_2:56499421-56499440 Msa1256500:CDS 55.0%
! GAGGCCAGCTGTTGATTGGT+GGG + chr8_2:56499422-56499441 Msa1256500:CDS 55.0%
TCAGCCCACCAATCAACAGC+TGG - chr8_2:56499429-56499448 None:intergenic 55.0%
TAACACACCCCCTCACACTC+AGG + chr8_2:56499683-56499702 Msa1256500:intron 55.0%
TGTCCAGTCCTGAGTGTGAG+GGG - chr8_2:56499694-56499713 None:intergenic 55.0%
! TTTTGGGCGTGAATGGGGTG+GGG - chr8_2:56499844-56499863 None:intergenic 55.0%
!! GTTTTGGGCGTGAATGGGGT+GGG - chr8_2:56499845-56499864 None:intergenic 55.0%
!! TGTTTTGGGCGTGAATGGGG+TGG - chr8_2:56499846-56499865 None:intergenic 55.0%
ACCCCATTCACGCCCAAAAC+AGG + chr8_2:56499845-56499864 Msa1256500:intron 55.0%
! GGACATCTAGAGCGTGACAC+GGG + chr8_2:56499866-56499885 Msa1256500:intron 55.0%
TCTACCAGGTCACGTGCAAC+TGG - chr8_2:56499895-56499914 None:intergenic 55.0%
GCACGTGACCTGGTAGAAAG+TGG + chr8_2:56499898-56499917 Msa1256500:intron 55.0%
! CTGTTGGGCCACTTTCTACC+AGG - chr8_2:56499909-56499928 None:intergenic 55.0%
CCCACGCTGCACAAAACAAC+AGG + chr8_2:56501300-56501319 Msa1256500:CDS 55.0%
!!! GCCTGTTGTTTTGTGCAGCG+TGG - chr8_2:56501304-56501323 None:intergenic 55.0%
CAGGCTTCCTGCCCAAAATG+TGG + chr8_2:56501319-56501338 Msa1256500:CDS 55.0%
GCAACGTCTTCGCGACAACA+TGG + chr8_2:56501364-56501383 Msa1256500:CDS 55.0%
CGTCTTCGCGACAACATGGA+TGG + chr8_2:56501368-56501387 Msa1256500:CDS 55.0%
!! ATGGATGGCACTGGCAGCAT+AGG + chr8_2:56501383-56501402 Msa1256500:CDS 55.0%
CGCCTTCAACACCTGACAAG+AGG + chr8_2:56501414-56501433 Msa1256500:CDS 55.0%
CTCCTTCCCGCAACCGCAAA+GGG - chr8_2:56498439-56498458 None:intergenic 60.0%
TGGCCTCTGGTGAGAAGCAG+TGG - chr8_2:56499409-56499428 None:intergenic 60.0%
GTCCAGTCCTGAGTGTGAGG+GGG - chr8_2:56499693-56499712 None:intergenic 60.0%
TTTGGGCGTGAATGGGGTGG+GGG - chr8_2:56499843-56499862 None:intergenic 60.0%
GTAGCCAGTTGCACGTGACC+TGG + chr8_2:56499888-56499907 Msa1256500:intron 60.0%
GCGCCTCTCGTGATAGCTTC+TGG - chr8_2:56501277-56501296 None:intergenic 60.0%
CGCGACAACATGGATGGCAC+TGG + chr8_2:56501374-56501393 Msa1256500:CDS 60.0%
GACCCTTTGCGGTTGCGGGA+AGG + chr8_2:56498434-56498453 Msa1256500:five_prime_UTR 65.0%
CCTCCTTCCCGCAACCGCAA+AGG - chr8_2:56498440-56498459 None:intergenic 65.0%
CCTTTGCGGTTGCGGGAAGG+AGG + chr8_2:56498437-56498456 Msa1256500:five_prime_UTR 65.0%
CACCCCCTCACACTCAGGAC+TGG + chr8_2:56499688-56499707 Msa1256500:intron 65.0%
TTGGGCGTGAATGGGGTGGG+GGG - chr8_2:56499842-56499861 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 56498301 56501959 56498301 ID=Msa1256500;Name=Msa1256500
chr8_2 mRNA 56498301 56501959 56498301 ID=Msa1256500-mRNA-1;Parent=Msa1256500;Name=Msa1256500-mRNA-1;_AED=0.10;_eAED=0.10;_QI=175|1|1|1|1|1|5|193|596
chr8_2 exon 56498301 56498495 56498301 ID=Msa1256500-mRNA-1:exon:17436;Parent=Msa1256500-mRNA-1
chr8_2 exon 56498612 56498681 56498612 ID=Msa1256500-mRNA-1:exon:17437;Parent=Msa1256500-mRNA-1
chr8_2 exon 56498949 56499575 56498949 ID=Msa1256500-mRNA-1:exon:17438;Parent=Msa1256500-mRNA-1
chr8_2 exon 56500198 56500722 56500198 ID=Msa1256500-mRNA-1:exon:17439;Parent=Msa1256500-mRNA-1
chr8_2 exon 56501218 56501959 56501218 ID=Msa1256500-mRNA-1:exon:17440;Parent=Msa1256500-mRNA-1
chr8_2 five_prime_UTR 56498301 56498475 56498301 ID=Msa1256500-mRNA-1:five_prime_utr;Parent=Msa1256500-mRNA-1
chr8_2 CDS 56498476 56498495 56498476 ID=Msa1256500-mRNA-1:cds;Parent=Msa1256500-mRNA-1
chr8_2 CDS 56498612 56498681 56498612 ID=Msa1256500-mRNA-1:cds;Parent=Msa1256500-mRNA-1
chr8_2 CDS 56498949 56499575 56498949 ID=Msa1256500-mRNA-1:cds;Parent=Msa1256500-mRNA-1
chr8_2 CDS 56500198 56500722 56500198 ID=Msa1256500-mRNA-1:cds;Parent=Msa1256500-mRNA-1
chr8_2 CDS 56501218 56501766 56501218 ID=Msa1256500-mRNA-1:cds;Parent=Msa1256500-mRNA-1
chr8_2 three_prime_UTR 56501767 56501959 56501767 ID=Msa1256500-mRNA-1:three_prime_utr;Parent=Msa1256500-mRNA-1
Gene Sequence

>Msa1256500

ATGAAGAGAACAAGTGAATGGAATTACTCCCAGGAGATTCCAAGTGATGTCACTATTCAAATTGGTGAAGCTTCCTATTCGTTACACAAGTTTCCATTAATTTCCAAAAGTGGACACATAAGGAAGCTCATTTCAGAATCTAGTGATGCTGTTGCTGATGTTCCATTAATTGAACTCATCGATGTTCCTGGTGGATCAGAAGCATTTGAACTAGCAGCAAAGTTCTGTTATGGGATAAACTTCGAAATAAGTGTCGAAAACATCGCTATGCTTCGATGTGTGGCCGAGTATCTTGAAATGACAGAAGATTATTCGGTCGGAAATTTGGTAGGAAGAACTGATTCTTACTTGAATGAAGTTGCACTAAAGAGCATGTCAGGGGCAATATCCATCTTACATACATCAGAAACTCTTCTTCCAATTGCAGAGAAAGCAAAATTAGTTAGCAGATGCATAGATGCAATAGCCTACATAGCTTCCAAGGAGAGTCAGTTTGGTTCGTCTGGTCGAAGTGATGGTAGTAGCAATGAAAGAGTGATGAATTCTTCCACTGCTTCTCACCAGAGGCCAGCTGTTGATTGGTGGGCTGAAGATCTAACAGTTCTTAGAATTGACTTTTTCCAAAGAGTTCTAATTGCAATGATGGCTAGAGGGTTTAAAAATTGTGGTATTGGTCCTATTATAATGCTTTATTCACAGAAATCTCTGAGAGGTTTGGAGATATTTGGAAAGGGAAGGAAGGATATAGAACCACATGAAGAGCATGAGAAGAGACTTATTTTAGAGATACTAGTGAGCCTTATGCCAAGGGAGAAGAATGCAATATCTGTCAGCTTTCTTTCACTATGGCTTAAGGCAGCAATATATCTTGAGACAACTGTTGCTTGCAGGTTTGATTTGGAGAGAAGGATAGCCATGCAATTAGGACAGGCTGTGTTGGATGATCTTCTTTTTCCTTCATTTAGCGGCGACACATCGTTTGATGTAGAAACAGTGCAAAGAATCATGATGAATTACCTTGAATCTGAAGTTGAAAATCATTACAATGCAGATGATGAGTATTATTCTTCTCCACAAAGGGATATTTTTCGGGTTGGAAAGTTATTGGAGAGCTACCTTGCTGAAATAGCTACTGATAGAAATTTATCAGTTTCAAAGTTTATCTGTCTTGCTGAATTAATGCCTGAAAAATCAAGAACAACAGAAGATGGAATGTATAGAGCCGTAGACATCTATCTTAAGGCACATCCATTTGTAAGCGACACGGACAGAAAGAAAGTTTGCAGCATGATGGATTTCCAGAAGCTATCACGAGAGGCGCGAACCCACGCTGCACAAAACAACAGGCTTCCTGCCCAAAATGTGGCTCAAATTCTCTATTATGAACAGCAACGTCTTCGCGACAACATGGATGGCACTGGCAGCATAGGTTCAGATTCGCCTTCAACACCTGACAAGAGGAATGTATACTCATCCGAGCTTTATCCAGCTTCTAATGAGGTTTCCAAATTAAGAAGAGAAAATGAAGAACTGAAATTAGAGATAGTGAAACTTAAAATAAAAATAAAAGAGACGGAAAAATGTACAGTACTTGAATCACCTATCAATAGTCCTTTGTTAAATGCATCATCACCTTCAGCTGATAAGCCTCCTTTGCCTAGAAAGTCATTCCTAAACTCAGTTTCAAGAAAACTTGGCCGTCTTTCTCCTTTTCAACGTGCTCATCCTGTCATAGCACGTCCCAAAGATCGCATACAACTAGACAAGATTCGACGCTACTCCATATCCTGA
Protein sequence

>Msa1256500

MKRTSEWNYSQEIPSDVTIQIGEASYSLHKFPLISKSGHIRKLISESSDAVADVPLIELIDVPGGSEAFELAAKFCYGINFEISVENIAMLRCVAEYLEMTEDYSVGNLVGRTDSYLNEVALKSMSGAISILHTSETLLPIAEKAKLVSRCIDAIAYIASKESQFGSSGRSDGSSNERVMNSSTASHQRPAVDWWAEDLTVLRIDFFQRVLIAMMARGFKNCGIGPIIMLYSQKSLRGLEIFGKGRKDIEPHEEHEKRLILEILVSLMPREKNAISVSFLSLWLKAAIYLETTVACRFDLERRIAMQLGQAVLDDLLFPSFSGDTSFDVETVQRIMMNYLESEVENHYNADDEYYSSPQRDIFRVGKLLESYLAEIATDRNLSVSKFICLAELMPEKSRTTEDGMYRAVDIYLKAHPFVSDTDRKKVCSMMDFQKLSREARTHAAQNNRLPAQNVAQILYYEQQRLRDNMDGTGSIGSDSPSTPDKRNVYSSELYPASNEVSKLRRENEELKLEIVKLKIKIKETEKCTVLESPINSPLLNASSPSADKPPLPRKSFLNSVSRKLGRLSPFQRAHPVIARPKDRIQLDKIRRYSIS*