AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1259430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1259430 MtrunA17_Chr4g0059621 61.538 91 1 1 1 91 1 57 2.46e-28 97.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 321 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGATGAAGGCCACATTCC+TGG 0.321908 8_2:-61072689 Msa1259430:CDS
TTTGTGTTGTGAATCGAAAA+TGG 0.327426 8_2:-61074189 Msa1259430:exon
CTACCAGATTCCGCTCTTCC+AGG 0.342832 8_2:+61072671 Msa1259420:CDS
CTTATATGCCGAAGACTTGA+TGG 0.346929 8_2:-61072126 Msa1259430:three_prime_UTR
ATGGGAATAGCTGGTAAGAT+TGG 0.362277 8_2:-61074170 Msa1259430:CDS
TGGGAATAGCTGGTAAGATT+GGG 0.374359 8_2:-61074169 Msa1259430:CDS
TCATGATGGTCATTTAGTTA+TGG 0.392302 8_2:+61072227 Msa1259420:intron
GATTGGGGATTACACTTTCA+AGG 0.398797 8_2:-61074153 Msa1259430:CDS
GGAAAACATGTGCTTCTGAC+TGG 0.421568 8_2:-61072745 Msa1259430:CDS
TAGTTTGTGAAGCTCATTGA+TGG 0.425613 8_2:-61072018 Msa1259430:three_prime_UTR
AGAAAATTCCATCAAGTCTT+CGG 0.429917 8_2:+61072118 Msa1259420:intron
AAGGCCTTTGTAAACATTAA+CGG 0.435862 8_2:+61074079 None:intergenic
AGCACGAAGGGGTAGGATAT+CGG 0.446184 8_2:-61072708 Msa1259430:CDS
TTGTGTTGTGAATCGAAAAT+GGG 0.473456 8_2:-61074188 Msa1259430:exon
ATTCCTGGAAGAGCGGAATC+TGG 0.476413 8_2:-61072674 Msa1259430:intron
CGAGAGAGTATAACTTCGTA+AGG 0.492734 8_2:-61074240 None:intergenic
GAATCGAAAATGGGAATAGC+TGG 0.500649 8_2:-61074179 Msa1259430:exon
ATGAGCTTCACAAACTAGAC+AGG 0.501110 8_2:+61072024 Msa1259420:intron
ATGTTTACAAAGGCCTTGCT+TGG 0.507155 8_2:-61074073 Msa1259430:CDS
TTCGATTCACAACACAAACT+AGG 0.520675 8_2:+61074194 None:intergenic
AAACAGATTATTACTCATGA+TGG 0.524302 8_2:+61072213 Msa1259420:intron
AAGGCTTTCACAGCAACTCT+CGG 0.525587 8_2:-61074134 Msa1259430:CDS
CTCAAGAGGATAAGCACGAA+GGG 0.539467 8_2:-61072720 Msa1259430:CDS
GGGAATAGCTGGTAAGATTG+GGG 0.543838 8_2:-61074168 Msa1259430:CDS
AAACTTGATGTGGAGGATGC+TGG 0.558872 8_2:-61072361 Msa1259430:CDS
AACATTAACGGAGAAAGTGC+CGG 0.561590 8_2:+61074091 None:intergenic
TTCTCCGTTAATGTTTACAA+AGG 0.561828 8_2:-61074083 Msa1259430:CDS
TTCGTAAGGGTGTGTGAGCG+TGG 0.563753 8_2:-61074226 Msa1259430:five_prime_UTR
GAAAACATGTGCTTCTGACT+GGG 0.569142 8_2:-61072744 Msa1259430:CDS
GAGGATAAGCACGAAGGGGT+AGG 0.576514 8_2:-61072715 Msa1259430:CDS
CTCTTTGCAGAAACTTGATG+TGG 0.577042 8_2:-61072371 Msa1259430:intron
ATTGAGCTTTGTGCCAAGCA+AGG 0.577529 8_2:+61074060 None:intergenic
ATCACTACTATCTACCTCAC+CGG 0.580585 8_2:-61074110 Msa1259430:CDS
ATTCCGCTCTTCCAGGAATG+TGG 0.580842 8_2:+61072678 Msa1259420:CDS
AGGGGTAGGATATCGGATGA+AGG 0.602365 8_2:-61072701 Msa1259430:CDS
TTTGCAGAAACTTGATGTGG+AGG 0.608728 8_2:-61072368 Msa1259430:intron
TGTGGAGGATGCTGGTGAGA+AGG 0.614238 8_2:-61072353 Msa1259430:CDS
GACTTGTAATTGAAGAAAGT+AGG 0.615746 8_2:+61072308 Msa1259420:intron
GCTTCTGACTGGGTCTCAAG+AGG 0.631693 8_2:-61072734 Msa1259430:CDS
TAACGGAGAAAGTGCCGGTG+AGG 0.652823 8_2:+61074096 None:intergenic
TGAGCTTCACAAACTAGACA+GGG 0.673877 8_2:+61072025 Msa1259420:intron
AGGCCACATTCCTGGAAGAG+CGG 0.674681 8_2:-61072681 Msa1259430:CDS
GCATGTGGTATACAGTAGAG+TGG 0.676264 8_2:-61072086 Msa1259430:three_prime_UTR
TCTCAAGAGGATAAGCACGA+AGG 0.683711 8_2:-61072721 Msa1259430:CDS
GAGAGAGTATAACTTCGTAA+GGG 0.686879 8_2:-61074239 None:intergenic
TCAAGAGGATAAGCACGAAG+GGG 0.708603 8_2:-61072719 Msa1259430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTACTAAATATGAATTAAT+TGG + chr8_2:61072282-61072301 Msa1259420:intron 15.0%
!! AGCTATAATATATTTATAGA+AGG - chr8_2:61072909-61072928 Msa1259430:intron 15.0%
!! AGTACTAAATATGAATTAAT+TGG + chr8_2:61072282-61072301 Msa1259420:intron 15.0%
!! AGCTATAATATATTTATAGA+AGG - chr8_2:61072909-61072928 Msa1259430:intron 15.0%
!! GTATAAAATATGAATGATCA+AGG + chr8_2:61072393-61072412 Msa1259420:intron 20.0%
!! ACAATTAATTGTTCAATAGT+TGG - chr8_2:61072411-61072430 Msa1259430:intron 20.0%
!! CAATTAATTGTTCAATAGTT+GGG - chr8_2:61072412-61072431 Msa1259430:intron 20.0%
!! AATTAATTGTTCAATAGTTG+GGG - chr8_2:61072413-61072432 Msa1259430:intron 20.0%
!!! TTTTCTCTTTTATTCAACAT+GGG + chr8_2:61072806-61072825 None:intergenic 20.0%
!!! CTGATAATTTTTAACTTGAT+TGG - chr8_2:61073990-61074009 Msa1259430:intron 20.0%
!! GTATAAAATATGAATGATCA+AGG + chr8_2:61072393-61072412 Msa1259420:intron 20.0%
!! ACAATTAATTGTTCAATAGT+TGG - chr8_2:61072411-61072430 Msa1259430:intron 20.0%
!! CAATTAATTGTTCAATAGTT+GGG - chr8_2:61072412-61072431 Msa1259430:intron 20.0%
!! AATTAATTGTTCAATAGTTG+GGG - chr8_2:61072413-61072432 Msa1259430:intron 20.0%
!!! TTTTCTCTTTTATTCAACAT+GGG + chr8_2:61072806-61072825 None:intergenic 20.0%
!!! CTGATAATTTTTAACTTGAT+TGG - chr8_2:61073990-61074009 Msa1259430:intron 20.0%
! GAAGATTGAAACAAAGTAAT+TGG + chr8_2:61072253-61072272 Msa1259420:intron 25.0%
!! TGTAGAATTGTTACTTGATT+TGG - chr8_2:61072349-61072368 Msa1259430:CDS 25.0%
!! ATGTCATAAACTGATTTCAT+AGG + chr8_2:61072442-61072461 Msa1259420:intron 25.0%
!! CTTTTCTCTTTTATTCAACA+TGG + chr8_2:61072807-61072826 None:intergenic 25.0%
! ACTAGGAAGGTTTATTATAT+AGG - chr8_2:61072865-61072884 Msa1259430:intron 25.0%
!!! ATTGATTTTCTCTTCAACAA+TGG - chr8_2:61073117-61073136 Msa1259430:intron 25.0%
! ATAAATAAGAGATCAAGATC+AGG - chr8_2:61073650-61073669 Msa1259430:intron 25.0%
! ATCCATAAATATTGTACTTG+CGG + chr8_2:61073759-61073778 None:intergenic 25.0%
! AAACAGATTATTACTCATGA+TGG + chr8_2:61074031-61074050 None:intergenic 25.0%
! GAAGATTGAAACAAAGTAAT+TGG + chr8_2:61072253-61072272 Msa1259420:intron 25.0%
!! TGTAGAATTGTTACTTGATT+TGG - chr8_2:61072349-61072368 Msa1259430:CDS 25.0%
!! ATGTCATAAACTGATTTCAT+AGG + chr8_2:61072442-61072461 Msa1259420:intron 25.0%
!! CTTTTCTCTTTTATTCAACA+TGG + chr8_2:61072807-61072826 None:intergenic 25.0%
! ACTAGGAAGGTTTATTATAT+AGG - chr8_2:61072865-61072884 Msa1259430:intron 25.0%
!!! ATTGATTTTCTCTTCAACAA+TGG - chr8_2:61073117-61073136 Msa1259430:intron 25.0%
! ATAAATAAGAGATCAAGATC+AGG - chr8_2:61073650-61073669 Msa1259430:intron 25.0%
! ATCCATAAATATTGTACTTG+CGG + chr8_2:61073759-61073778 None:intergenic 25.0%
! AAACAGATTATTACTCATGA+TGG + chr8_2:61074031-61074050 None:intergenic 25.0%
TTTGTGTTGTGAATCGAAAA+TGG - chr8_2:61072052-61072071 Msa1259430:three_prime_UTR 30.0%
TTGTGTTGTGAATCGAAAAT+GGG - chr8_2:61072053-61072072 Msa1259430:three_prime_UTR 30.0%
TTCTCCGTTAATGTTTACAA+AGG - chr8_2:61072158-61072177 Msa1259430:three_prime_UTR 30.0%
AAGGCCTTTGTAAACATTAA+CGG + chr8_2:61072165-61072184 Msa1259420:intron 30.0%
! TCTCTTTTCATAAGCTATCT+TGG + chr8_2:61072502-61072521 Msa1259420:intron 30.0%
ATAAGCTCTCTTAGAAATTG+TGG - chr8_2:61072595-61072614 Msa1259430:intron 30.0%
AATAGTCCTGGATAAGATAT+GGG + chr8_2:61072695-61072714 Msa1259420:CDS 30.0%
AAATAGTCCTGGATAAGATA+TGG + chr8_2:61072696-61072715 Msa1259420:CDS 30.0%
! ATCTAGAGTGTGGATATAAA+CGG - chr8_2:61072745-61072764 Msa1259430:CDS 30.0%
! TAACAAAAATGCCTTGCTTT+TGG + chr8_2:61073073-61073092 None:intergenic 30.0%
!! AGCAGCATTTTAGAAGAATT+TGG + chr8_2:61073175-61073194 None:intergenic 30.0%
! TCTAAAATGCTGCTAAAGTA+TGG - chr8_2:61073181-61073200 Msa1259430:intron 30.0%
CTAAAATGCTGCTAAAGTAT+GGG - chr8_2:61073182-61073201 Msa1259430:intron 30.0%
GACTTGTAATTGAAGAAAGT+AGG + chr8_2:61073936-61073955 None:intergenic 30.0%
TCATGATGGTCATTTAGTTA+TGG + chr8_2:61074017-61074036 None:intergenic 30.0%
AGAAAATTCCATCAAGTCTT+CGG + chr8_2:61074126-61074145 None:intergenic 30.0%
! TTTTCTTTTCAACATGCATG+TGG - chr8_2:61074140-61074159 Msa1259430:CDS 30.0%
TTTGTGTTGTGAATCGAAAA+TGG - chr8_2:61072052-61072071 Msa1259430:three_prime_UTR 30.0%
TTGTGTTGTGAATCGAAAAT+GGG - chr8_2:61072053-61072072 Msa1259430:three_prime_UTR 30.0%
TTCTCCGTTAATGTTTACAA+AGG - chr8_2:61072158-61072177 Msa1259430:three_prime_UTR 30.0%
AAGGCCTTTGTAAACATTAA+CGG + chr8_2:61072165-61072184 Msa1259420:intron 30.0%
! TCTCTTTTCATAAGCTATCT+TGG + chr8_2:61072502-61072521 Msa1259420:intron 30.0%
ATAAGCTCTCTTAGAAATTG+TGG - chr8_2:61072595-61072614 Msa1259430:intron 30.0%
AATAGTCCTGGATAAGATAT+GGG + chr8_2:61072695-61072714 Msa1259420:CDS 30.0%
AAATAGTCCTGGATAAGATA+TGG + chr8_2:61072696-61072715 Msa1259420:CDS 30.0%
! ATCTAGAGTGTGGATATAAA+CGG - chr8_2:61072745-61072764 Msa1259430:CDS 30.0%
! TAACAAAAATGCCTTGCTTT+TGG + chr8_2:61073073-61073092 None:intergenic 30.0%
!! AGCAGCATTTTAGAAGAATT+TGG + chr8_2:61073175-61073194 None:intergenic 30.0%
! TCTAAAATGCTGCTAAAGTA+TGG - chr8_2:61073181-61073200 Msa1259430:intron 30.0%
CTAAAATGCTGCTAAAGTAT+GGG - chr8_2:61073182-61073201 Msa1259430:intron 30.0%
GACTTGTAATTGAAGAAAGT+AGG + chr8_2:61073936-61073955 None:intergenic 30.0%
TCATGATGGTCATTTAGTTA+TGG + chr8_2:61074017-61074036 None:intergenic 30.0%
AGAAAATTCCATCAAGTCTT+CGG + chr8_2:61074126-61074145 None:intergenic 30.0%
! TTTTCTTTTCAACATGCATG+TGG - chr8_2:61074140-61074159 Msa1259430:CDS 30.0%
TTCGATTCACAACACAAACT+AGG + chr8_2:61072050-61072069 Msa1259420:intron 35.0%
CTCTCTTAGAAATTGTGGTT+GGG - chr8_2:61072600-61072619 Msa1259430:intron 35.0%
TAGCTGTTTATCCAAAAGCA+AGG - chr8_2:61073059-61073078 Msa1259430:intron 35.0%
CTCTGTGTTGGGTTAATATT+TGG - chr8_2:61073218-61073237 Msa1259430:intron 35.0%
TGTGGACGAGATATTCATTT+CGG - chr8_2:61073369-61073388 Msa1259430:intron 35.0%
TTCTGAATCTATCTCGGATA+CGG + chr8_2:61073446-61073465 None:intergenic 35.0%
AATGGCTTCTGAATCTATCT+CGG + chr8_2:61073452-61073471 None:intergenic 35.0%
AGATCAGGTTACTATCATCA+TGG - chr8_2:61073665-61073684 Msa1259430:intron 35.0%
TACATTAAAGACCTCTAGCT+TGG - chr8_2:61073699-61073718 Msa1259430:intron 35.0%
CACCGCAAGTACAATATTTA+TGG - chr8_2:61073754-61073773 Msa1259430:intron 35.0%
! TAGTTTGTGAAGCTCATTGA+TGG - chr8_2:61074223-61074242 Msa1259430:five_prime_UTR 35.0%
TTCGATTCACAACACAAACT+AGG + chr8_2:61072050-61072069 Msa1259420:intron 35.0%
CTCTCTTAGAAATTGTGGTT+GGG - chr8_2:61072600-61072619 Msa1259430:intron 35.0%
TAGCTGTTTATCCAAAAGCA+AGG - chr8_2:61073059-61073078 Msa1259430:intron 35.0%
CTCTGTGTTGGGTTAATATT+TGG - chr8_2:61073218-61073237 Msa1259430:intron 35.0%
TGTGGACGAGATATTCATTT+CGG - chr8_2:61073369-61073388 Msa1259430:intron 35.0%
TTCTGAATCTATCTCGGATA+CGG + chr8_2:61073446-61073465 None:intergenic 35.0%
AATGGCTTCTGAATCTATCT+CGG + chr8_2:61073452-61073471 None:intergenic 35.0%
AGATCAGGTTACTATCATCA+TGG - chr8_2:61073665-61073684 Msa1259430:intron 35.0%
TACATTAAAGACCTCTAGCT+TGG - chr8_2:61073699-61073718 Msa1259430:intron 35.0%
CACCGCAAGTACAATATTTA+TGG - chr8_2:61073754-61073773 Msa1259430:intron 35.0%
! TAGTTTGTGAAGCTCATTGA+TGG - chr8_2:61074223-61074242 Msa1259430:five_prime_UTR 35.0%
GAATCGAAAATGGGAATAGC+TGG - chr8_2:61072062-61072081 Msa1259430:three_prime_UTR 40.0%
ATGGGAATAGCTGGTAAGAT+TGG - chr8_2:61072071-61072090 Msa1259430:three_prime_UTR 40.0%
TGGGAATAGCTGGTAAGATT+GGG - chr8_2:61072072-61072091 Msa1259430:three_prime_UTR 40.0%
GATTGGGGATTACACTTTCA+AGG - chr8_2:61072088-61072107 Msa1259430:three_prime_UTR 40.0%
ATCACTACTATCTACCTCAC+CGG - chr8_2:61072131-61072150 Msa1259430:three_prime_UTR 40.0%
! AACATTAACGGAGAAAGTGC+CGG + chr8_2:61072153-61072172 Msa1259420:intron 40.0%
ATGTTTACAAAGGCCTTGCT+TGG - chr8_2:61072168-61072187 Msa1259430:three_prime_UTR 40.0%
AAGCTATCTTGGAGAGCTTA+CGG + chr8_2:61072491-61072510 Msa1259420:intron 40.0%
GCTCTCTTAGAAATTGTGGT+TGG - chr8_2:61072599-61072618 Msa1259430:intron 40.0%
CTTACACAATCGCACAAAAC+CGG - chr8_2:61072624-61072643 Msa1259430:intron 40.0%
TAGAGTGTGGATATAAACGG+TGG - chr8_2:61072748-61072767 Msa1259430:CDS 40.0%
ACTCTCTTTCAAGATCCACT+AGG + chr8_2:61072778-61072797 None:intergenic 40.0%
AGCTTACTCTCTAAGCTACT+TGG + chr8_2:61072893-61072912 None:intergenic 40.0%
AATGCTGCTAAAGTATGGGT+TGG - chr8_2:61073186-61073205 Msa1259430:intron 40.0%
! TATTATGACAGAGGGGTATG+GGG + chr8_2:61073263-61073282 None:intergenic 40.0%
! CTATTATGACAGAGGGGTAT+GGG + chr8_2:61073264-61073283 None:intergenic 40.0%
TCTATTATGACAGAGGGGTA+TGG + chr8_2:61073265-61073284 None:intergenic 40.0%
CTACGTCTATTATGACAGAG+GGG + chr8_2:61073270-61073289 None:intergenic 40.0%
GCTACGTCTATTATGACAGA+GGG + chr8_2:61073271-61073290 None:intergenic 40.0%
AGACATGTGATGGCTTAAGT+TGG - chr8_2:61073314-61073333 Msa1259430:intron 40.0%
GACATGTGATGGCTTAAGTT+GGG - chr8_2:61073315-61073334 Msa1259430:intron 40.0%
GTGGACGAGATATTCATTTC+GGG - chr8_2:61073370-61073389 Msa1259430:intron 40.0%
TCTGAATCTATCTCGGATAC+GGG + chr8_2:61073445-61073464 None:intergenic 40.0%
! GGACTGAGTCAAAAGCATAA+TGG + chr8_2:61073470-61073489 None:intergenic 40.0%
! AAGCACATGTTTTCCATGTG+GGG + chr8_2:61073491-61073510 None:intergenic 40.0%
! GAAGCACATGTTTTCCATGT+GGG + chr8_2:61073492-61073511 None:intergenic 40.0%
!! AGAAGCACATGTTTTCCATG+TGG + chr8_2:61073493-61073512 None:intergenic 40.0%
GAAAACATGTGCTTCTGACT+GGG - chr8_2:61073497-61073516 Msa1259430:intron 40.0%
TTAGGCTTAATCTCGGATGT+GGG + chr8_2:61073596-61073615 None:intergenic 40.0%
TTTAGGCTTAATCTCGGATG+TGG + chr8_2:61073597-61073616 None:intergenic 40.0%
!! AACGGCTTTAGGCTTAATCT+CGG + chr8_2:61073603-61073622 None:intergenic 40.0%
CACATCAAGTTTCTGCAAAG+AGG + chr8_2:61073872-61073891 None:intergenic 40.0%
CTCTTTGCAGAAACTTGATG+TGG - chr8_2:61073870-61073889 Msa1259430:intron 40.0%
TTTGCAGAAACTTGATGTGG+AGG - chr8_2:61073873-61073892 Msa1259430:intron 40.0%
CTTATATGCCGAAGACTTGA+TGG - chr8_2:61074115-61074134 Msa1259430:CDS 40.0%
TGAGCTTCACAAACTAGACA+GGG + chr8_2:61074219-61074238 None:intergenic 40.0%
ATGAGCTTCACAAACTAGAC+AGG + chr8_2:61074220-61074239 None:intergenic 40.0%
GAATCGAAAATGGGAATAGC+TGG - chr8_2:61072062-61072081 Msa1259430:three_prime_UTR 40.0%
ATGGGAATAGCTGGTAAGAT+TGG - chr8_2:61072071-61072090 Msa1259430:three_prime_UTR 40.0%
TGGGAATAGCTGGTAAGATT+GGG - chr8_2:61072072-61072091 Msa1259430:three_prime_UTR 40.0%
GATTGGGGATTACACTTTCA+AGG - chr8_2:61072088-61072107 Msa1259430:three_prime_UTR 40.0%
ATCACTACTATCTACCTCAC+CGG - chr8_2:61072131-61072150 Msa1259430:three_prime_UTR 40.0%
! AACATTAACGGAGAAAGTGC+CGG + chr8_2:61072153-61072172 Msa1259420:intron 40.0%
ATGTTTACAAAGGCCTTGCT+TGG - chr8_2:61072168-61072187 Msa1259430:three_prime_UTR 40.0%
AAGCTATCTTGGAGAGCTTA+CGG + chr8_2:61072491-61072510 Msa1259420:intron 40.0%
GCTCTCTTAGAAATTGTGGT+TGG - chr8_2:61072599-61072618 Msa1259430:intron 40.0%
CTTACACAATCGCACAAAAC+CGG - chr8_2:61072624-61072643 Msa1259430:intron 40.0%
TAGAGTGTGGATATAAACGG+TGG - chr8_2:61072748-61072767 Msa1259430:CDS 40.0%
ACTCTCTTTCAAGATCCACT+AGG + chr8_2:61072778-61072797 None:intergenic 40.0%
AGCTTACTCTCTAAGCTACT+TGG + chr8_2:61072893-61072912 None:intergenic 40.0%
AATGCTGCTAAAGTATGGGT+TGG - chr8_2:61073186-61073205 Msa1259430:intron 40.0%
! TATTATGACAGAGGGGTATG+GGG + chr8_2:61073263-61073282 None:intergenic 40.0%
! CTATTATGACAGAGGGGTAT+GGG + chr8_2:61073264-61073283 None:intergenic 40.0%
TCTATTATGACAGAGGGGTA+TGG + chr8_2:61073265-61073284 None:intergenic 40.0%
CTACGTCTATTATGACAGAG+GGG + chr8_2:61073270-61073289 None:intergenic 40.0%
GCTACGTCTATTATGACAGA+GGG + chr8_2:61073271-61073290 None:intergenic 40.0%
AGACATGTGATGGCTTAAGT+TGG - chr8_2:61073314-61073333 Msa1259430:intron 40.0%
GACATGTGATGGCTTAAGTT+GGG - chr8_2:61073315-61073334 Msa1259430:intron 40.0%
GTGGACGAGATATTCATTTC+GGG - chr8_2:61073370-61073389 Msa1259430:intron 40.0%
TCTGAATCTATCTCGGATAC+GGG + chr8_2:61073445-61073464 None:intergenic 40.0%
! GGACTGAGTCAAAAGCATAA+TGG + chr8_2:61073470-61073489 None:intergenic 40.0%
! AAGCACATGTTTTCCATGTG+GGG + chr8_2:61073491-61073510 None:intergenic 40.0%
! GAAGCACATGTTTTCCATGT+GGG + chr8_2:61073492-61073511 None:intergenic 40.0%
!! AGAAGCACATGTTTTCCATG+TGG + chr8_2:61073493-61073512 None:intergenic 40.0%
GAAAACATGTGCTTCTGACT+GGG - chr8_2:61073497-61073516 Msa1259430:intron 40.0%
TTAGGCTTAATCTCGGATGT+GGG + chr8_2:61073596-61073615 None:intergenic 40.0%
TTTAGGCTTAATCTCGGATG+TGG + chr8_2:61073597-61073616 None:intergenic 40.0%
!! AACGGCTTTAGGCTTAATCT+CGG + chr8_2:61073603-61073622 None:intergenic 40.0%
CACATCAAGTTTCTGCAAAG+AGG + chr8_2:61073872-61073891 None:intergenic 40.0%
CTCTTTGCAGAAACTTGATG+TGG - chr8_2:61073870-61073889 Msa1259430:intron 40.0%
TTTGCAGAAACTTGATGTGG+AGG - chr8_2:61073873-61073892 Msa1259430:intron 40.0%
CTTATATGCCGAAGACTTGA+TGG - chr8_2:61074115-61074134 Msa1259430:CDS 40.0%
TGAGCTTCACAAACTAGACA+GGG + chr8_2:61074219-61074238 None:intergenic 40.0%
ATGAGCTTCACAAACTAGAC+AGG + chr8_2:61074220-61074239 None:intergenic 40.0%
GGGAATAGCTGGTAAGATTG+GGG - chr8_2:61072073-61072092 Msa1259430:three_prime_UTR 45.0%
AAGGCTTTCACAGCAACTCT+CGG - chr8_2:61072107-61072126 Msa1259430:three_prime_UTR 45.0%
! ATTGAGCTTTGTGCCAAGCA+AGG + chr8_2:61072184-61072203 Msa1259420:intron 45.0%
GAGAGAGAGAAAGAGGGTTA+CGG + chr8_2:61072207-61072226 Msa1259420:intron 45.0%
GAGAGAGAGAGAGAGAAAGA+GGG + chr8_2:61072213-61072232 Msa1259420:intron 45.0%
TGAGAGAGAGAGAGAGAAAG+AGG + chr8_2:61072214-61072233 Msa1259420:intron 45.0%
TGGCAGTGTGTTTATAAGCG+GGG + chr8_2:61072671-61072690 Msa1259420:CDS 45.0%
CTGGCAGTGTGTTTATAAGC+GGG + chr8_2:61072672-61072691 Msa1259420:CDS 45.0%
GCTGGCAGTGTGTTTATAAG+CGG + chr8_2:61072673-61072692 Msa1259420:CDS 45.0%
TATCCACACTCTAGATGTCC+AGG + chr8_2:61072741-61072760 Msa1259420:CDS 45.0%
GTGTGGATATAAACGGTGGA+TGG - chr8_2:61072752-61072771 Msa1259430:CDS 45.0%
TCGTGTGACTGAATAGCACT+AGG - chr8_2:61072848-61072867 Msa1259430:intron 45.0%
GTGACTGAATAGCACTAGGA+AGG - chr8_2:61072852-61072871 Msa1259430:intron 45.0%
TGGTCTTCATGTCTCTGTGT+TGG - chr8_2:61073206-61073225 Msa1259430:intron 45.0%
GGTCTTCATGTCTCTGTGTT+GGG - chr8_2:61073207-61073226 Msa1259430:intron 45.0%
! ATTATGACAGAGGGGTATGG+GGG + chr8_2:61073262-61073281 None:intergenic 45.0%
GGCTACGTCTATTATGACAG+AGG + chr8_2:61073272-61073291 None:intergenic 45.0%
TCACGTGACTAGGTGATGAT+GGG + chr8_2:61073293-61073312 None:intergenic 45.0%
TTCACGTGACTAGGTGATGA+TGG + chr8_2:61073294-61073313 None:intergenic 45.0%
CACATGTCTTTCACGTGACT+AGG + chr8_2:61073303-61073322 None:intergenic 45.0%
GTCACGTGAAAGACATGTGA+TGG - chr8_2:61073304-61073323 Msa1259430:intron 45.0%
!! TTAAGTTGGGACAGACGAGT+TGG - chr8_2:61073328-61073347 Msa1259430:intron 45.0%
GATATTCATTTCGGGAGCGA+AGG - chr8_2:61073378-61073397 Msa1259430:intron 45.0%
TCCTTTATGGGTCATGACAG+GGG + chr8_2:61073408-61073427 None:intergenic 45.0%
CTCCTTTATGGGTCATGACA+GGG + chr8_2:61073409-61073428 None:intergenic 45.0%
TGTCATGACCCATAAAGGAG+CGG - chr8_2:61073409-61073428 Msa1259430:intron 45.0%
GGAAAACATGTGCTTCTGAC+TGG - chr8_2:61073496-61073515 Msa1259430:intron 45.0%
TCTCAAGAGGATAAGCACGA+AGG - chr8_2:61073520-61073539 Msa1259430:intron 45.0%
CTCAAGAGGATAAGCACGAA+GGG - chr8_2:61073521-61073540 Msa1259430:intron 45.0%
TCAAGAGGATAAGCACGAAG+GGG - chr8_2:61073522-61073541 Msa1259430:intron 45.0%
TACAATGGTATAGACCCTGC+CGG + chr8_2:61073732-61073751 None:intergenic 45.0%
TGTACTTGCGGTGAGTACAA+TGG + chr8_2:61073747-61073766 None:intergenic 45.0%
AAACTTGATGTGGAGGATGC+TGG - chr8_2:61073880-61073899 Msa1259430:intron 45.0%
GCATGTGGTATACAGTAGAG+TGG - chr8_2:61074155-61074174 Msa1259430:CDS 45.0%
GGGAATAGCTGGTAAGATTG+GGG - chr8_2:61072073-61072092 Msa1259430:three_prime_UTR 45.0%
AAGGCTTTCACAGCAACTCT+CGG - chr8_2:61072107-61072126 Msa1259430:three_prime_UTR 45.0%
! ATTGAGCTTTGTGCCAAGCA+AGG + chr8_2:61072184-61072203 Msa1259420:intron 45.0%
GAGAGAGAGAAAGAGGGTTA+CGG + chr8_2:61072207-61072226 Msa1259420:intron 45.0%
GAGAGAGAGAGAGAGAAAGA+GGG + chr8_2:61072213-61072232 Msa1259420:intron 45.0%
TGAGAGAGAGAGAGAGAAAG+AGG + chr8_2:61072214-61072233 Msa1259420:intron 45.0%
TGGCAGTGTGTTTATAAGCG+GGG + chr8_2:61072671-61072690 Msa1259420:CDS 45.0%
CTGGCAGTGTGTTTATAAGC+GGG + chr8_2:61072672-61072691 Msa1259420:CDS 45.0%
GCTGGCAGTGTGTTTATAAG+CGG + chr8_2:61072673-61072692 Msa1259420:CDS 45.0%
TATCCACACTCTAGATGTCC+AGG + chr8_2:61072741-61072760 Msa1259420:CDS 45.0%
GTGTGGATATAAACGGTGGA+TGG - chr8_2:61072752-61072771 Msa1259430:CDS 45.0%
TCGTGTGACTGAATAGCACT+AGG - chr8_2:61072848-61072867 Msa1259430:intron 45.0%
GTGACTGAATAGCACTAGGA+AGG - chr8_2:61072852-61072871 Msa1259430:intron 45.0%
TGGTCTTCATGTCTCTGTGT+TGG - chr8_2:61073206-61073225 Msa1259430:intron 45.0%
GGTCTTCATGTCTCTGTGTT+GGG - chr8_2:61073207-61073226 Msa1259430:intron 45.0%
! ATTATGACAGAGGGGTATGG+GGG + chr8_2:61073262-61073281 None:intergenic 45.0%
GGCTACGTCTATTATGACAG+AGG + chr8_2:61073272-61073291 None:intergenic 45.0%
TCACGTGACTAGGTGATGAT+GGG + chr8_2:61073293-61073312 None:intergenic 45.0%
TTCACGTGACTAGGTGATGA+TGG + chr8_2:61073294-61073313 None:intergenic 45.0%
CACATGTCTTTCACGTGACT+AGG + chr8_2:61073303-61073322 None:intergenic 45.0%
GTCACGTGAAAGACATGTGA+TGG - chr8_2:61073304-61073323 Msa1259430:intron 45.0%
!! TTAAGTTGGGACAGACGAGT+TGG - chr8_2:61073328-61073347 Msa1259430:intron 45.0%
GATATTCATTTCGGGAGCGA+AGG - chr8_2:61073378-61073397 Msa1259430:intron 45.0%
TCCTTTATGGGTCATGACAG+GGG + chr8_2:61073408-61073427 None:intergenic 45.0%
CTCCTTTATGGGTCATGACA+GGG + chr8_2:61073409-61073428 None:intergenic 45.0%
TGTCATGACCCATAAAGGAG+CGG - chr8_2:61073409-61073428 Msa1259430:intron 45.0%
GGAAAACATGTGCTTCTGAC+TGG - chr8_2:61073496-61073515 Msa1259430:intron 45.0%
TCTCAAGAGGATAAGCACGA+AGG - chr8_2:61073520-61073539 Msa1259430:intron 45.0%
CTCAAGAGGATAAGCACGAA+GGG - chr8_2:61073521-61073540 Msa1259430:intron 45.0%
TCAAGAGGATAAGCACGAAG+GGG - chr8_2:61073522-61073541 Msa1259430:intron 45.0%
TACAATGGTATAGACCCTGC+CGG + chr8_2:61073732-61073751 None:intergenic 45.0%
TGTACTTGCGGTGAGTACAA+TGG + chr8_2:61073747-61073766 None:intergenic 45.0%
AAACTTGATGTGGAGGATGC+TGG - chr8_2:61073880-61073899 Msa1259430:intron 45.0%
GCATGTGGTATACAGTAGAG+TGG - chr8_2:61074155-61074174 Msa1259430:CDS 45.0%
AATCCTCACCTCTCGTAAGC+CGG + chr8_2:61072646-61072665 Msa1259420:CDS 50.0%
GGCAGTGTGTTTATAAGCGG+GGG + chr8_2:61072670-61072689 Msa1259420:CDS 50.0%
TGCCAGCCCATATCTTATCC+AGG - chr8_2:61072686-61072705 Msa1259430:CDS 50.0%
GTCCTGGATAAGATATGGGC+TGG + chr8_2:61072691-61072710 Msa1259420:CDS 50.0%
! GGGGGTGTGTTAAATAGTCC+TGG + chr8_2:61072707-61072726 Msa1259420:CDS 50.0%
ATAAACGGTGGATGGCCTAG+TGG - chr8_2:61072760-61072779 Msa1259430:intron 50.0%
CCTTTATGGGTCATGACAGG+GGG + chr8_2:61073407-61073426 None:intergenic 50.0%
GCTCCTTTATGGGTCATGAC+AGG + chr8_2:61073410-61073429 None:intergenic 50.0%
ACTAGGCTCCGCTCCTTTAT+GGG + chr8_2:61073420-61073439 None:intergenic 50.0%
TATCTCGGATACGGGTCACT+AGG + chr8_2:61073437-61073456 None:intergenic 50.0%
!! CTTTTGACTCAGTCCCCACA+TGG - chr8_2:61073475-61073494 Msa1259430:intron 50.0%
AGCACGAAGGGGTAGGATAT+CGG - chr8_2:61073533-61073552 Msa1259430:intron 50.0%
AGGGGTAGGATATCGGATGA+AGG - chr8_2:61073540-61073559 Msa1259430:intron 50.0%
ATTCCGCTCTTCCAGGAATG+TGG + chr8_2:61073566-61073585 None:intergenic 50.0%
ATTCCTGGAAGAGCGGAATC+TGG - chr8_2:61073567-61073586 Msa1259430:intron 50.0%
! ATGCCTAGTGAGAAGCGCAA+CGG + chr8_2:61073621-61073640 None:intergenic 50.0%
AATCCTCACCTCTCGTAAGC+CGG + chr8_2:61072646-61072665 Msa1259420:CDS 50.0%
GGCAGTGTGTTTATAAGCGG+GGG + chr8_2:61072670-61072689 Msa1259420:CDS 50.0%
TGCCAGCCCATATCTTATCC+AGG - chr8_2:61072686-61072705 Msa1259430:CDS 50.0%
GTCCTGGATAAGATATGGGC+TGG + chr8_2:61072691-61072710 Msa1259420:CDS 50.0%
! GGGGGTGTGTTAAATAGTCC+TGG + chr8_2:61072707-61072726 Msa1259420:CDS 50.0%
ATAAACGGTGGATGGCCTAG+TGG - chr8_2:61072760-61072779 Msa1259430:intron 50.0%
CCTTTATGGGTCATGACAGG+GGG + chr8_2:61073407-61073426 None:intergenic 50.0%
GCTCCTTTATGGGTCATGAC+AGG + chr8_2:61073410-61073429 None:intergenic 50.0%
ACTAGGCTCCGCTCCTTTAT+GGG + chr8_2:61073420-61073439 None:intergenic 50.0%
TATCTCGGATACGGGTCACT+AGG + chr8_2:61073437-61073456 None:intergenic 50.0%
!! CTTTTGACTCAGTCCCCACA+TGG - chr8_2:61073475-61073494 Msa1259430:intron 50.0%
AGCACGAAGGGGTAGGATAT+CGG - chr8_2:61073533-61073552 Msa1259430:intron 50.0%
AGGGGTAGGATATCGGATGA+AGG - chr8_2:61073540-61073559 Msa1259430:intron 50.0%
ATTCCGCTCTTCCAGGAATG+TGG + chr8_2:61073566-61073585 None:intergenic 50.0%
ATTCCTGGAAGAGCGGAATC+TGG - chr8_2:61073567-61073586 Msa1259430:intron 50.0%
! ATGCCTAGTGAGAAGCGCAA+CGG + chr8_2:61073621-61073640 None:intergenic 50.0%
! TTCGTAAGGGTGTGTGAGCG+TGG - chr8_2:61072015-61072034 Msa1259430:three_prime_UTR 55.0%
!! TAACGGAGAAAGTGCCGGTG+AGG + chr8_2:61072148-61072167 Msa1259420:intron 55.0%
! GCACAAAACCGGCTTACGAG+AGG - chr8_2:61072635-61072654 Msa1259430:intron 55.0%
! AAACCGGCTTACGAGAGGTG+AGG - chr8_2:61072640-61072659 Msa1259430:intron 55.0%
TAACACACCCCCTCACGTTC+AGG - chr8_2:61072715-61072734 Msa1259430:CDS 55.0%
ATGTCCAGGCCTGAACGTGA+GGG + chr8_2:61072727-61072746 Msa1259420:CDS 55.0%
! AGGCCTGGACATCTAGAGTG+TGG - chr8_2:61072735-61072754 Msa1259430:CDS 55.0%
!! GTTGGGACAGACGAGTTGGT+TGG - chr8_2:61073332-61073351 Msa1259430:intron 55.0%
TTGGCCACATGCTCGACATG+TGG - chr8_2:61073351-61073370 Msa1259430:intron 55.0%
TCGTCCACATGTCGAGCATG+TGG + chr8_2:61073358-61073377 None:intergenic 55.0%
CCCCCTGTCATGACCCATAA+AGG - chr8_2:61073404-61073423 Msa1259430:intron 55.0%
CACTAGGCTCCGCTCCTTTA+TGG + chr8_2:61073421-61073440 None:intergenic 55.0%
GCTTCTGACTGGGTCTCAAG+AGG - chr8_2:61073507-61073526 Msa1259430:intron 55.0%
GAGGATAAGCACGAAGGGGT+AGG - chr8_2:61073526-61073545 Msa1259430:intron 55.0%
TCGGATGAAGGCCACATTCC+TGG - chr8_2:61073552-61073571 Msa1259430:intron 55.0%
AGGCCACATTCCTGGAAGAG+CGG - chr8_2:61073560-61073579 Msa1259430:intron 55.0%
CTACCAGATTCCGCTCTTCC+AGG + chr8_2:61073573-61073592 None:intergenic 55.0%
! GTGAGAAGCGCAACGGCTTT+AGG + chr8_2:61073614-61073633 None:intergenic 55.0%
AAGCCGTTGCGCTTCTCACT+AGG - chr8_2:61073615-61073634 Msa1259430:intron 55.0%
! CCTCTAGCTTGGTACATCGC+CGG - chr8_2:61073710-61073729 Msa1259430:intron 55.0%
TGTGGAGGATGCTGGTGAGA+AGG - chr8_2:61073888-61073907 Msa1259430:intron 55.0%
ACGCTCACACACCCTTACGA+AGG + chr8_2:61072016-61072035 Msa1259420:intron 55.0%
! TTCGTAAGGGTGTGTGAGCG+TGG - chr8_2:61072015-61072034 Msa1259430:three_prime_UTR 55.0%
!! TAACGGAGAAAGTGCCGGTG+AGG + chr8_2:61072148-61072167 Msa1259420:intron 55.0%
! GCACAAAACCGGCTTACGAG+AGG - chr8_2:61072635-61072654 Msa1259430:intron 55.0%
! AAACCGGCTTACGAGAGGTG+AGG - chr8_2:61072640-61072659 Msa1259430:intron 55.0%
TAACACACCCCCTCACGTTC+AGG - chr8_2:61072715-61072734 Msa1259430:CDS 55.0%
ATGTCCAGGCCTGAACGTGA+GGG + chr8_2:61072727-61072746 Msa1259420:CDS 55.0%
! AGGCCTGGACATCTAGAGTG+TGG - chr8_2:61072735-61072754 Msa1259430:CDS 55.0%
!! GTTGGGACAGACGAGTTGGT+TGG - chr8_2:61073332-61073351 Msa1259430:intron 55.0%
TTGGCCACATGCTCGACATG+TGG - chr8_2:61073351-61073370 Msa1259430:intron 55.0%
TCGTCCACATGTCGAGCATG+TGG + chr8_2:61073358-61073377 None:intergenic 55.0%
CCCCCTGTCATGACCCATAA+AGG - chr8_2:61073404-61073423 Msa1259430:intron 55.0%
CACTAGGCTCCGCTCCTTTA+TGG + chr8_2:61073421-61073440 None:intergenic 55.0%
GCTTCTGACTGGGTCTCAAG+AGG - chr8_2:61073507-61073526 Msa1259430:intron 55.0%
GAGGATAAGCACGAAGGGGT+AGG - chr8_2:61073526-61073545 Msa1259430:intron 55.0%
TCGGATGAAGGCCACATTCC+TGG - chr8_2:61073552-61073571 Msa1259430:intron 55.0%
AGGCCACATTCCTGGAAGAG+CGG - chr8_2:61073560-61073579 Msa1259430:intron 55.0%
CTACCAGATTCCGCTCTTCC+AGG + chr8_2:61073573-61073592 None:intergenic 55.0%
! GTGAGAAGCGCAACGGCTTT+AGG + chr8_2:61073614-61073633 None:intergenic 55.0%
AAGCCGTTGCGCTTCTCACT+AGG - chr8_2:61073615-61073634 Msa1259430:intron 55.0%
! CCTCTAGCTTGGTACATCGC+CGG - chr8_2:61073710-61073729 Msa1259430:intron 55.0%
TGTGGAGGATGCTGGTGAGA+AGG - chr8_2:61073888-61073907 Msa1259430:intron 55.0%
TGTCCAGGCCTGAACGTGAG+GGG + chr8_2:61072726-61072745 Msa1259420:CDS 60.0%
GATGTCCAGGCCTGAACGTG+AGG + chr8_2:61072728-61072747 Msa1259420:CDS 60.0%
CCGGCGATGTACCAAGCTAG+AGG + chr8_2:61073713-61073732 None:intergenic 60.0%
TAGCTTGGTACATCGCCGGC+AGG - chr8_2:61073714-61073733 Msa1259430:intron 60.0%
AGCTTGGTACATCGCCGGCA+GGG - chr8_2:61073715-61073734 Msa1259430:intron 60.0%
TGTCCAGGCCTGAACGTGAG+GGG + chr8_2:61072726-61072745 Msa1259420:CDS 60.0%
GATGTCCAGGCCTGAACGTG+AGG + chr8_2:61072728-61072747 Msa1259420:CDS 60.0%
CCGGCGATGTACCAAGCTAG+AGG + chr8_2:61073713-61073732 None:intergenic 60.0%
TAGCTTGGTACATCGCCGGC+AGG - chr8_2:61073714-61073733 Msa1259430:intron 60.0%
AGCTTGGTACATCGCCGGCA+GGG - chr8_2:61073715-61073734 Msa1259430:intron 60.0%
GTCCAGGCCTGAACGTGAGG+GGG + chr8_2:61072725-61072744 Msa1259420:CDS 65.0%
GTCCAGGCCTGAACGTGAGG+GGG + chr8_2:61072725-61072744 Msa1259420:CDS 65.0%
CACCCCCTCACGTTCAGGCC+TGG - chr8_2:61072720-61072739 Msa1259430:CDS 70.0%
CACCCCCTCACGTTCAGGCC+TGG - chr8_2:61072720-61072739 Msa1259430:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 61072015 61074248 61072015 ID=Msa1259430;Name=Msa1259430
chr8_2 mRNA 61072015 61074248 61072015 ID=Msa1259430-mRNA-1;Parent=Msa1259430;Name=Msa1259430-mRNA-1;_AED=0.14;_eAED=0.14;_QI=56|0|0.66|0.66|1|1|3|330|91
chr8_2 exon 61072015 61072383 61072015 ID=Msa1259430-mRNA-1:exon:19197;Parent=Msa1259430-mRNA-1
chr8_2 exon 61072675 61072776 61072675 ID=Msa1259430-mRNA-1:exon:19196;Parent=Msa1259430-mRNA-1
chr8_2 exon 61074058 61074248 61074058 ID=Msa1259430-mRNA-1:exon:19195;Parent=Msa1259430-mRNA-1
chr8_2 five_prime_UTR 61074193 61074248 61074193 ID=Msa1259430-mRNA-1:five_prime_utr;Parent=Msa1259430-mRNA-1
chr8_2 CDS 61074058 61074192 61074058 ID=Msa1259430-mRNA-1:cds;Parent=Msa1259430-mRNA-1
chr8_2 CDS 61072675 61072776 61072675 ID=Msa1259430-mRNA-1:cds;Parent=Msa1259430-mRNA-1
chr8_2 CDS 61072345 61072383 61072345 ID=Msa1259430-mRNA-1:cds;Parent=Msa1259430-mRNA-1
chr8_2 three_prime_UTR 61072015 61072344 61072015 ID=Msa1259430-mRNA-1:three_prime_utr;Parent=Msa1259430-mRNA-1
Gene Sequence

>Msa1259430

ATGGGAATAGCTGGTAAGATTGGGGATTACACTTTCAAGGCTTTCACAGCAACTCTCGGCATCACTACTATCTACCTCACCGGCACTTTCTCCGTTAATGTTTACAAAGGCCTTGCTTGGCACAAAGCTCAATCCTCCCCACATGGAAAACATGTGCTTCTGACTGGGTCTCAAGAGGATAAGCACGAAGGGGTAGGATATCGGATGAAGGCCACATTCCTGGAAGAGCGGAATCTGAAACTTGATGTGGAGGATGCTGGTGAGAAGGCTTCGTGA
Protein sequence

>Msa1259430

MGIAGKIGDYTFKAFTATLGITTIYLTGTFSVNVYKGLAWHKAQSSPHGKHVLLTGSQEDKHEGVGYRMKATFLEERNLKLDVEDAGEKAS*