AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1299040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1299040 MtrunA17_Chr4g0059621 61.538 91 1 1 1 91 1 57 2.46e-28 97.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 323 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGTGTTGTGAATCGAAAA+TGG 0.317556 8_3:-60037590 Msa1299040:exon
TCGGATGAAGGCCACATTCC+TGG 0.321908 8_3:-60036088 Msa1299040:CDS
CTACCAGATTCCGCTCTTCC+AGG 0.342832 8_3:+60036070 Msa1299030:CDS
CTTATATGCCGAAGACTTGA+TGG 0.346929 8_3:-60035525 Msa1299040:three_prime_UTR
ATGGGAATAGCTGGTAAGAT+TGG 0.362277 8_3:-60037571 Msa1299040:CDS
TGGGAATAGCTGGTAAGATT+GGG 0.374359 8_3:-60037570 Msa1299040:CDS
TCATGATGGTCATTTAGTTA+TGG 0.392302 8_3:+60035626 Msa1299030:intron
GATTGGGGATTACACTTTCA+AGG 0.398797 8_3:-60037554 Msa1299040:CDS
AGCACGAAGGGGTGGGATAT+CGG 0.405578 8_3:-60036107 Msa1299040:CDS
TCGATTCACAACACAAACTA+AGG 0.419316 8_3:+60037596 None:intergenic
GGAAAACATGTGCTTCTGAC+TGG 0.421568 8_3:-60036144 Msa1299040:CDS
TAGTTTGTGAAGCTCATTGA+TGG 0.425613 8_3:-60035417 Msa1299040:three_prime_UTR
AGAAAATTCCATCAAGTCTT+CGG 0.429917 8_3:+60035517 Msa1299030:intron
AAGGCCTTTGTAAACATTAA+CGG 0.435862 8_3:+60037480 None:intergenic
TTGTGTTGTGAATCGAAAAT+GGG 0.473456 8_3:-60037589 Msa1299040:exon
ATTCCTGGAAGAGCGGAATC+TGG 0.476413 8_3:-60036073 Msa1299040:intron
AACGAGAGTATAACTTCGTA+AGG 0.489487 8_3:-60037642 None:intergenic
GAATCGAAAATGGGAATAGC+TGG 0.500649 8_3:-60037580 Msa1299040:exon
ATGAGCTTCACAAACTAGAC+AGG 0.501110 8_3:+60035423 Msa1299030:intron
ATGTTTACAAAGGCCTTGCT+TGG 0.507155 8_3:-60037474 Msa1299040:CDS
AAACAGATTATTACTCATGA+TGG 0.524302 8_3:+60035612 Msa1299030:intron
AAGGCTTTCACAGCAACTCT+CGG 0.525587 8_3:-60037535 Msa1299040:CDS
CTCAAGAGGATAAGCACGAA+GGG 0.539467 8_3:-60036119 Msa1299040:CDS
GGGAATAGCTGGTAAGATTG+GGG 0.543838 8_3:-60037569 Msa1299040:CDS
AAACTTGATGTGGAGGATGC+TGG 0.558872 8_3:-60035760 Msa1299040:CDS
AACATTAACGGAGAAAGTGC+CGG 0.561590 8_3:+60037492 None:intergenic
TTCTCCGTTAATGTTTACAA+AGG 0.561828 8_3:-60037484 Msa1299040:CDS
TTCGTAAGGGTGTGTGAGCG+TGG 0.563753 8_3:-60037628 Msa1299040:five_prime_UTR
GAAAACATGTGCTTCTGACT+GGG 0.569142 8_3:-60036143 Msa1299040:CDS
AGAGGATAAGCACGAAGGGG+TGG 0.569221 8_3:-60036115 Msa1299040:CDS
GAGGATAAGCACGAAGGGGT+GGG 0.571327 8_3:-60036114 Msa1299040:CDS
AGGGGTGGGATATCGGATGA+AGG 0.573010 8_3:-60036100 Msa1299040:CDS
CTCTTTGCAGAAACTTGATG+TGG 0.577042 8_3:-60035770 Msa1299040:intron
ATTGAGCTTTGTGCCAAGCA+AGG 0.577529 8_3:+60037461 None:intergenic
ATCACTACTATCTACCTCAC+CGG 0.580585 8_3:-60037511 Msa1299040:CDS
ATTCCGCTCTTCCAGGAATG+TGG 0.580842 8_3:+60036077 Msa1299030:CDS
TTTGCAGAAACTTGATGTGG+AGG 0.608728 8_3:-60035767 Msa1299040:intron
TGTGGAGGATGCTGGTGAGA+AGG 0.614238 8_3:-60035752 Msa1299040:CDS
GACTTGTAATTGAAGAAAGT+AGG 0.615746 8_3:+60035707 Msa1299030:intron
GCTTCTGACTGGGTCTCAAG+AGG 0.631693 8_3:-60036133 Msa1299040:CDS
TAACGGAGAAAGTGCCGGTG+AGG 0.652823 8_3:+60037497 None:intergenic
TGAGCTTCACAAACTAGACA+GGG 0.673877 8_3:+60035424 Msa1299030:intron
AGGCCACATTCCTGGAAGAG+CGG 0.674681 8_3:-60036080 Msa1299040:CDS
GCATGTGGTATACAGTAGAG+TGG 0.676264 8_3:-60035485 Msa1299040:three_prime_UTR
ACGAGAGTATAACTTCGTAA+GGG 0.678863 8_3:-60037641 None:intergenic
TCTCAAGAGGATAAGCACGA+AGG 0.683711 8_3:-60036120 Msa1299040:CDS
TCAAGAGGATAAGCACGAAG+GGG 0.708603 8_3:-60036118 Msa1299040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTACTAAATATGAATTAAT+TGG + chr8_3:60035684-60035703 Msa1299030:intron 15.0%
!! AGCTATAATATATTTATAGA+AGG - chr8_3:60036311-60036330 Msa1299040:intron 15.0%
!! AGTACTAAATATGAATTAAT+TGG + chr8_3:60035684-60035703 Msa1299030:intron 15.0%
!! AGCTATAATATATTTATAGA+AGG - chr8_3:60036311-60036330 Msa1299040:intron 15.0%
!! GTATAAAATATGAATGATCA+AGG + chr8_3:60035795-60035814 Msa1299030:intron 20.0%
!! ACAATTAATTGTTCAATAGT+TGG - chr8_3:60035813-60035832 Msa1299040:intron 20.0%
!! CAATTAATTGTTCAATAGTT+GGG - chr8_3:60035814-60035833 Msa1299040:intron 20.0%
!! AATTAATTGTTCAATAGTTG+GGG - chr8_3:60035815-60035834 Msa1299040:intron 20.0%
!!! TTTTCTCTTTTATTCAACAT+GGG + chr8_3:60036208-60036227 None:intergenic 20.0%
!!! CTGATAATTTTTAACTTGAT+TGG - chr8_3:60037392-60037411 Msa1299040:intron 20.0%
!! GTATAAAATATGAATGATCA+AGG + chr8_3:60035795-60035814 Msa1299030:intron 20.0%
!! ACAATTAATTGTTCAATAGT+TGG - chr8_3:60035813-60035832 Msa1299040:intron 20.0%
!! CAATTAATTGTTCAATAGTT+GGG - chr8_3:60035814-60035833 Msa1299040:intron 20.0%
!! AATTAATTGTTCAATAGTTG+GGG - chr8_3:60035815-60035834 Msa1299040:intron 20.0%
!!! TTTTCTCTTTTATTCAACAT+GGG + chr8_3:60036208-60036227 None:intergenic 20.0%
!!! CTGATAATTTTTAACTTGAT+TGG - chr8_3:60037392-60037411 Msa1299040:intron 20.0%
! GAAGATTGAAACAAAGTAAT+TGG + chr8_3:60035655-60035674 Msa1299030:intron 25.0%
!! TGTAGAATTGTTACTTGATT+TGG - chr8_3:60035751-60035770 Msa1299040:CDS 25.0%
!! ATGTCATAAACTGATTTCAT+AGG + chr8_3:60035844-60035863 Msa1299030:intron 25.0%
!! CTTTTCTCTTTTATTCAACA+TGG + chr8_3:60036209-60036228 None:intergenic 25.0%
! ACTAGGAAGGTTTATTATAT+AGG - chr8_3:60036267-60036286 Msa1299040:intron 25.0%
!!! ATTGATTTTCTCTTCAACAA+TGG - chr8_3:60036519-60036538 Msa1299040:intron 25.0%
! ATAAATAAGAGATCAAGATC+AGG - chr8_3:60037052-60037071 Msa1299040:intron 25.0%
! ATCCATAAATATTGTACTTG+CGG + chr8_3:60037161-60037180 None:intergenic 25.0%
! AAACAGATTATTACTCATGA+TGG + chr8_3:60037433-60037452 None:intergenic 25.0%
! GAAGATTGAAACAAAGTAAT+TGG + chr8_3:60035655-60035674 Msa1299030:intron 25.0%
!! TGTAGAATTGTTACTTGATT+TGG - chr8_3:60035751-60035770 Msa1299040:CDS 25.0%
!! ATGTCATAAACTGATTTCAT+AGG + chr8_3:60035844-60035863 Msa1299030:intron 25.0%
!! CTTTTCTCTTTTATTCAACA+TGG + chr8_3:60036209-60036228 None:intergenic 25.0%
! ACTAGGAAGGTTTATTATAT+AGG - chr8_3:60036267-60036286 Msa1299040:intron 25.0%
!!! ATTGATTTTCTCTTCAACAA+TGG - chr8_3:60036519-60036538 Msa1299040:intron 25.0%
! ATAAATAAGAGATCAAGATC+AGG - chr8_3:60037052-60037071 Msa1299040:intron 25.0%
! ATCCATAAATATTGTACTTG+CGG + chr8_3:60037161-60037180 None:intergenic 25.0%
! AAACAGATTATTACTCATGA+TGG + chr8_3:60037433-60037452 None:intergenic 25.0%
TTTGTGTTGTGAATCGAAAA+TGG - chr8_3:60035452-60035471 Msa1299040:three_prime_UTR 30.0%
TTGTGTTGTGAATCGAAAAT+GGG - chr8_3:60035453-60035472 Msa1299040:three_prime_UTR 30.0%
TTCTCCGTTAATGTTTACAA+AGG - chr8_3:60035558-60035577 Msa1299040:three_prime_UTR 30.0%
AAGGCCTTTGTAAACATTAA+CGG + chr8_3:60035565-60035584 Msa1299030:intron 30.0%
! TCTCTTTTCATAAGCTATCT+TGG + chr8_3:60035904-60035923 Msa1299030:intron 30.0%
ATAAGCTCTCTTAGAAATTG+TGG - chr8_3:60035997-60036016 Msa1299040:intron 30.0%
AATAGTCCTGGATAAGATAT+GGG + chr8_3:60036097-60036116 Msa1299030:CDS 30.0%
AAATAGTCCTGGATAAGATA+TGG + chr8_3:60036098-60036117 Msa1299030:CDS 30.0%
! ATCTAGAGTGTGGATATAAA+CGG - chr8_3:60036147-60036166 Msa1299040:CDS 30.0%
! TAACAAAAATGCCTTGCTTT+TGG + chr8_3:60036475-60036494 None:intergenic 30.0%
!! AGCAGCATTTTAGAAGAATT+TGG + chr8_3:60036577-60036596 None:intergenic 30.0%
! TCTAAAATGCTGCTAAAGTA+TGG - chr8_3:60036583-60036602 Msa1299040:intron 30.0%
CTAAAATGCTGCTAAAGTAT+GGG - chr8_3:60036584-60036603 Msa1299040:intron 30.0%
GACTTGTAATTGAAGAAAGT+AGG + chr8_3:60037338-60037357 None:intergenic 30.0%
TCATGATGGTCATTTAGTTA+TGG + chr8_3:60037419-60037438 None:intergenic 30.0%
AGAAAATTCCATCAAGTCTT+CGG + chr8_3:60037528-60037547 None:intergenic 30.0%
! TTTTCTTTTCAACATGCATG+TGG - chr8_3:60037542-60037561 Msa1299040:CDS 30.0%
TTTGTGTTGTGAATCGAAAA+TGG - chr8_3:60035452-60035471 Msa1299040:three_prime_UTR 30.0%
TTGTGTTGTGAATCGAAAAT+GGG - chr8_3:60035453-60035472 Msa1299040:three_prime_UTR 30.0%
TTCTCCGTTAATGTTTACAA+AGG - chr8_3:60035558-60035577 Msa1299040:three_prime_UTR 30.0%
AAGGCCTTTGTAAACATTAA+CGG + chr8_3:60035565-60035584 Msa1299030:intron 30.0%
! TCTCTTTTCATAAGCTATCT+TGG + chr8_3:60035904-60035923 Msa1299030:intron 30.0%
ATAAGCTCTCTTAGAAATTG+TGG - chr8_3:60035997-60036016 Msa1299040:intron 30.0%
AATAGTCCTGGATAAGATAT+GGG + chr8_3:60036097-60036116 Msa1299030:CDS 30.0%
AAATAGTCCTGGATAAGATA+TGG + chr8_3:60036098-60036117 Msa1299030:CDS 30.0%
! ATCTAGAGTGTGGATATAAA+CGG - chr8_3:60036147-60036166 Msa1299040:CDS 30.0%
! TAACAAAAATGCCTTGCTTT+TGG + chr8_3:60036475-60036494 None:intergenic 30.0%
!! AGCAGCATTTTAGAAGAATT+TGG + chr8_3:60036577-60036596 None:intergenic 30.0%
! TCTAAAATGCTGCTAAAGTA+TGG - chr8_3:60036583-60036602 Msa1299040:intron 30.0%
CTAAAATGCTGCTAAAGTAT+GGG - chr8_3:60036584-60036603 Msa1299040:intron 30.0%
GACTTGTAATTGAAGAAAGT+AGG + chr8_3:60037338-60037357 None:intergenic 30.0%
TCATGATGGTCATTTAGTTA+TGG + chr8_3:60037419-60037438 None:intergenic 30.0%
AGAAAATTCCATCAAGTCTT+CGG + chr8_3:60037528-60037547 None:intergenic 30.0%
! TTTTCTTTTCAACATGCATG+TGG - chr8_3:60037542-60037561 Msa1299040:CDS 30.0%
TCGATTCACAACACAAACTA+AGG + chr8_3:60035449-60035468 Msa1299030:intron 35.0%
CTCTCTTAGAAATTGTGGTT+GGG - chr8_3:60036002-60036021 Msa1299040:intron 35.0%
TAGCTGTTTATCCAAAAGCA+AGG - chr8_3:60036461-60036480 Msa1299040:intron 35.0%
CTCTGTGTTGGGTTAATATT+TGG - chr8_3:60036620-60036639 Msa1299040:intron 35.0%
TGTGGACGAGATATTCATTT+CGG - chr8_3:60036771-60036790 Msa1299040:intron 35.0%
TTCTGAATCTATCTCGGATA+CGG + chr8_3:60036848-60036867 None:intergenic 35.0%
AATGGCTTCTGAATCTATCT+CGG + chr8_3:60036854-60036873 None:intergenic 35.0%
AGATCAGGTTACTATCATCA+TGG - chr8_3:60037067-60037086 Msa1299040:intron 35.0%
TACATTAAAGACCTCTAGCT+TGG - chr8_3:60037101-60037120 Msa1299040:intron 35.0%
CACCGCAAGTACAATATTTA+TGG - chr8_3:60037156-60037175 Msa1299040:intron 35.0%
! TAGTTTGTGAAGCTCATTGA+TGG - chr8_3:60037625-60037644 Msa1299040:five_prime_UTR 35.0%
TCGATTCACAACACAAACTA+AGG + chr8_3:60035449-60035468 Msa1299030:intron 35.0%
CTCTCTTAGAAATTGTGGTT+GGG - chr8_3:60036002-60036021 Msa1299040:intron 35.0%
TAGCTGTTTATCCAAAAGCA+AGG - chr8_3:60036461-60036480 Msa1299040:intron 35.0%
CTCTGTGTTGGGTTAATATT+TGG - chr8_3:60036620-60036639 Msa1299040:intron 35.0%
TGTGGACGAGATATTCATTT+CGG - chr8_3:60036771-60036790 Msa1299040:intron 35.0%
TTCTGAATCTATCTCGGATA+CGG + chr8_3:60036848-60036867 None:intergenic 35.0%
AATGGCTTCTGAATCTATCT+CGG + chr8_3:60036854-60036873 None:intergenic 35.0%
AGATCAGGTTACTATCATCA+TGG - chr8_3:60037067-60037086 Msa1299040:intron 35.0%
TACATTAAAGACCTCTAGCT+TGG - chr8_3:60037101-60037120 Msa1299040:intron 35.0%
CACCGCAAGTACAATATTTA+TGG - chr8_3:60037156-60037175 Msa1299040:intron 35.0%
! TAGTTTGTGAAGCTCATTGA+TGG - chr8_3:60037625-60037644 Msa1299040:five_prime_UTR 35.0%
GAATCGAAAATGGGAATAGC+TGG - chr8_3:60035462-60035481 Msa1299040:three_prime_UTR 40.0%
ATGGGAATAGCTGGTAAGAT+TGG - chr8_3:60035471-60035490 Msa1299040:three_prime_UTR 40.0%
TGGGAATAGCTGGTAAGATT+GGG - chr8_3:60035472-60035491 Msa1299040:three_prime_UTR 40.0%
GATTGGGGATTACACTTTCA+AGG - chr8_3:60035488-60035507 Msa1299040:three_prime_UTR 40.0%
ATCACTACTATCTACCTCAC+CGG - chr8_3:60035531-60035550 Msa1299040:three_prime_UTR 40.0%
! AACATTAACGGAGAAAGTGC+CGG + chr8_3:60035553-60035572 Msa1299030:intron 40.0%
ATGTTTACAAAGGCCTTGCT+TGG - chr8_3:60035568-60035587 Msa1299040:three_prime_UTR 40.0%
AAGCTATCTTGGAGAGCTTA+CGG + chr8_3:60035893-60035912 Msa1299030:intron 40.0%
GCTCTCTTAGAAATTGTGGT+TGG - chr8_3:60036001-60036020 Msa1299040:intron 40.0%
CTTACACAATCGCACAAAAC+CGG - chr8_3:60036026-60036045 Msa1299040:intron 40.0%
TAGAGTGTGGATATAAACGG+TGG - chr8_3:60036150-60036169 Msa1299040:CDS 40.0%
ACTCTCTTTCAAGATCCACT+AGG + chr8_3:60036180-60036199 None:intergenic 40.0%
AGCTTACTCTCTAAGCTACT+TGG + chr8_3:60036295-60036314 None:intergenic 40.0%
AATGCTGCTAAAGTATGGGT+TGG - chr8_3:60036588-60036607 Msa1299040:intron 40.0%
! TATTATGACAGAGGGGTATG+GGG + chr8_3:60036665-60036684 None:intergenic 40.0%
! CTATTATGACAGAGGGGTAT+GGG + chr8_3:60036666-60036685 None:intergenic 40.0%
TCTATTATGACAGAGGGGTA+TGG + chr8_3:60036667-60036686 None:intergenic 40.0%
CTACGTCTATTATGACAGAG+GGG + chr8_3:60036672-60036691 None:intergenic 40.0%
GCTACGTCTATTATGACAGA+GGG + chr8_3:60036673-60036692 None:intergenic 40.0%
AGACATGTGATGGCTTAAGT+TGG - chr8_3:60036716-60036735 Msa1299040:intron 40.0%
GACATGTGATGGCTTAAGTT+GGG - chr8_3:60036717-60036736 Msa1299040:intron 40.0%
GTGGACGAGATATTCATTTC+GGG - chr8_3:60036772-60036791 Msa1299040:intron 40.0%
TCTGAATCTATCTCGGATAC+GGG + chr8_3:60036847-60036866 None:intergenic 40.0%
! GGACTGAGTCAAAAGCATAA+TGG + chr8_3:60036872-60036891 None:intergenic 40.0%
! AAGCACATGTTTTCCATGTG+GGG + chr8_3:60036893-60036912 None:intergenic 40.0%
! GAAGCACATGTTTTCCATGT+GGG + chr8_3:60036894-60036913 None:intergenic 40.0%
!! AGAAGCACATGTTTTCCATG+TGG + chr8_3:60036895-60036914 None:intergenic 40.0%
GAAAACATGTGCTTCTGACT+GGG - chr8_3:60036899-60036918 Msa1299040:intron 40.0%
TTAGGCTTAATCTCGGATGT+GGG + chr8_3:60036998-60037017 None:intergenic 40.0%
TTTAGGCTTAATCTCGGATG+TGG + chr8_3:60036999-60037018 None:intergenic 40.0%
!! AACGGCTTTAGGCTTAATCT+CGG + chr8_3:60037005-60037024 None:intergenic 40.0%
CACATCAAGTTTCTGCAAAG+AGG + chr8_3:60037274-60037293 None:intergenic 40.0%
CTCTTTGCAGAAACTTGATG+TGG - chr8_3:60037272-60037291 Msa1299040:intron 40.0%
TTTGCAGAAACTTGATGTGG+AGG - chr8_3:60037275-60037294 Msa1299040:intron 40.0%
CTTATATGCCGAAGACTTGA+TGG - chr8_3:60037517-60037536 Msa1299040:CDS 40.0%
TGAGCTTCACAAACTAGACA+GGG + chr8_3:60037621-60037640 None:intergenic 40.0%
ATGAGCTTCACAAACTAGAC+AGG + chr8_3:60037622-60037641 None:intergenic 40.0%
GAATCGAAAATGGGAATAGC+TGG - chr8_3:60035462-60035481 Msa1299040:three_prime_UTR 40.0%
ATGGGAATAGCTGGTAAGAT+TGG - chr8_3:60035471-60035490 Msa1299040:three_prime_UTR 40.0%
TGGGAATAGCTGGTAAGATT+GGG - chr8_3:60035472-60035491 Msa1299040:three_prime_UTR 40.0%
GATTGGGGATTACACTTTCA+AGG - chr8_3:60035488-60035507 Msa1299040:three_prime_UTR 40.0%
ATCACTACTATCTACCTCAC+CGG - chr8_3:60035531-60035550 Msa1299040:three_prime_UTR 40.0%
! AACATTAACGGAGAAAGTGC+CGG + chr8_3:60035553-60035572 Msa1299030:intron 40.0%
ATGTTTACAAAGGCCTTGCT+TGG - chr8_3:60035568-60035587 Msa1299040:three_prime_UTR 40.0%
AAGCTATCTTGGAGAGCTTA+CGG + chr8_3:60035893-60035912 Msa1299030:intron 40.0%
GCTCTCTTAGAAATTGTGGT+TGG - chr8_3:60036001-60036020 Msa1299040:intron 40.0%
CTTACACAATCGCACAAAAC+CGG - chr8_3:60036026-60036045 Msa1299040:intron 40.0%
TAGAGTGTGGATATAAACGG+TGG - chr8_3:60036150-60036169 Msa1299040:CDS 40.0%
ACTCTCTTTCAAGATCCACT+AGG + chr8_3:60036180-60036199 None:intergenic 40.0%
AGCTTACTCTCTAAGCTACT+TGG + chr8_3:60036295-60036314 None:intergenic 40.0%
AATGCTGCTAAAGTATGGGT+TGG - chr8_3:60036588-60036607 Msa1299040:intron 40.0%
! TATTATGACAGAGGGGTATG+GGG + chr8_3:60036665-60036684 None:intergenic 40.0%
! CTATTATGACAGAGGGGTAT+GGG + chr8_3:60036666-60036685 None:intergenic 40.0%
TCTATTATGACAGAGGGGTA+TGG + chr8_3:60036667-60036686 None:intergenic 40.0%
CTACGTCTATTATGACAGAG+GGG + chr8_3:60036672-60036691 None:intergenic 40.0%
GCTACGTCTATTATGACAGA+GGG + chr8_3:60036673-60036692 None:intergenic 40.0%
AGACATGTGATGGCTTAAGT+TGG - chr8_3:60036716-60036735 Msa1299040:intron 40.0%
GACATGTGATGGCTTAAGTT+GGG - chr8_3:60036717-60036736 Msa1299040:intron 40.0%
GTGGACGAGATATTCATTTC+GGG - chr8_3:60036772-60036791 Msa1299040:intron 40.0%
TCTGAATCTATCTCGGATAC+GGG + chr8_3:60036847-60036866 None:intergenic 40.0%
! GGACTGAGTCAAAAGCATAA+TGG + chr8_3:60036872-60036891 None:intergenic 40.0%
! AAGCACATGTTTTCCATGTG+GGG + chr8_3:60036893-60036912 None:intergenic 40.0%
! GAAGCACATGTTTTCCATGT+GGG + chr8_3:60036894-60036913 None:intergenic 40.0%
!! AGAAGCACATGTTTTCCATG+TGG + chr8_3:60036895-60036914 None:intergenic 40.0%
GAAAACATGTGCTTCTGACT+GGG - chr8_3:60036899-60036918 Msa1299040:intron 40.0%
TTAGGCTTAATCTCGGATGT+GGG + chr8_3:60036998-60037017 None:intergenic 40.0%
TTTAGGCTTAATCTCGGATG+TGG + chr8_3:60036999-60037018 None:intergenic 40.0%
!! AACGGCTTTAGGCTTAATCT+CGG + chr8_3:60037005-60037024 None:intergenic 40.0%
CACATCAAGTTTCTGCAAAG+AGG + chr8_3:60037274-60037293 None:intergenic 40.0%
CTCTTTGCAGAAACTTGATG+TGG - chr8_3:60037272-60037291 Msa1299040:intron 40.0%
TTTGCAGAAACTTGATGTGG+AGG - chr8_3:60037275-60037294 Msa1299040:intron 40.0%
CTTATATGCCGAAGACTTGA+TGG - chr8_3:60037517-60037536 Msa1299040:CDS 40.0%
TGAGCTTCACAAACTAGACA+GGG + chr8_3:60037621-60037640 None:intergenic 40.0%
ATGAGCTTCACAAACTAGAC+AGG + chr8_3:60037622-60037641 None:intergenic 40.0%
GGGAATAGCTGGTAAGATTG+GGG - chr8_3:60035473-60035492 Msa1299040:three_prime_UTR 45.0%
AAGGCTTTCACAGCAACTCT+CGG - chr8_3:60035507-60035526 Msa1299040:three_prime_UTR 45.0%
! ATTGAGCTTTGTGCCAAGCA+AGG + chr8_3:60035584-60035603 Msa1299030:intron 45.0%
GAGAGAGAGAAAGAGGGTTA+CGG + chr8_3:60035607-60035626 Msa1299030:intron 45.0%
GAGAGAGAGAGAGAGAAAGA+GGG + chr8_3:60035613-60035632 Msa1299030:intron 45.0%
AGAGAGAGAGAGAGAGAAAG+AGG + chr8_3:60035614-60035633 Msa1299030:intron 45.0%
TGGCAGTGTGTTTATAAGCG+GGG + chr8_3:60036073-60036092 Msa1299030:CDS 45.0%
CTGGCAGTGTGTTTATAAGC+GGG + chr8_3:60036074-60036093 Msa1299030:CDS 45.0%
GCTGGCAGTGTGTTTATAAG+CGG + chr8_3:60036075-60036094 Msa1299030:CDS 45.0%
TATCCACACTCTAGATGTCC+AGG + chr8_3:60036143-60036162 Msa1299030:CDS 45.0%
GTGTGGATATAAACGGTGGA+TGG - chr8_3:60036154-60036173 Msa1299040:CDS 45.0%
TCGTGTGACTGAATAGCACT+AGG - chr8_3:60036250-60036269 Msa1299040:intron 45.0%
GTGACTGAATAGCACTAGGA+AGG - chr8_3:60036254-60036273 Msa1299040:intron 45.0%
TGGTCTTCATGTCTCTGTGT+TGG - chr8_3:60036608-60036627 Msa1299040:intron 45.0%
GGTCTTCATGTCTCTGTGTT+GGG - chr8_3:60036609-60036628 Msa1299040:intron 45.0%
! ATTATGACAGAGGGGTATGG+GGG + chr8_3:60036664-60036683 None:intergenic 45.0%
GGCTACGTCTATTATGACAG+AGG + chr8_3:60036674-60036693 None:intergenic 45.0%
TCACGTGACTAGGTGATGAT+GGG + chr8_3:60036695-60036714 None:intergenic 45.0%
TTCACGTGACTAGGTGATGA+TGG + chr8_3:60036696-60036715 None:intergenic 45.0%
CACATGTCTTTCACGTGACT+AGG + chr8_3:60036705-60036724 None:intergenic 45.0%
GTCACGTGAAAGACATGTGA+TGG - chr8_3:60036706-60036725 Msa1299040:intron 45.0%
!! TTAAGTTGGGACAGACGAGT+TGG - chr8_3:60036730-60036749 Msa1299040:intron 45.0%
GATATTCATTTCGGGAGCGA+AGG - chr8_3:60036780-60036799 Msa1299040:intron 45.0%
TCCTTTATGGGTCATGACAG+GGG + chr8_3:60036810-60036829 None:intergenic 45.0%
CTCCTTTATGGGTCATGACA+GGG + chr8_3:60036811-60036830 None:intergenic 45.0%
TGTCATGACCCATAAAGGAG+CGG - chr8_3:60036811-60036830 Msa1299040:intron 45.0%
GGAAAACATGTGCTTCTGAC+TGG - chr8_3:60036898-60036917 Msa1299040:intron 45.0%
TCTCAAGAGGATAAGCACGA+AGG - chr8_3:60036922-60036941 Msa1299040:intron 45.0%
CTCAAGAGGATAAGCACGAA+GGG - chr8_3:60036923-60036942 Msa1299040:intron 45.0%
TCAAGAGGATAAGCACGAAG+GGG - chr8_3:60036924-60036943 Msa1299040:intron 45.0%
TACAATGGTATAGACCCTGC+CGG + chr8_3:60037134-60037153 None:intergenic 45.0%
TGTACTTGCGGTGAGTACAA+TGG + chr8_3:60037149-60037168 None:intergenic 45.0%
AAACTTGATGTGGAGGATGC+TGG - chr8_3:60037282-60037301 Msa1299040:intron 45.0%
GCATGTGGTATACAGTAGAG+TGG - chr8_3:60037557-60037576 Msa1299040:CDS 45.0%
GGGAATAGCTGGTAAGATTG+GGG - chr8_3:60035473-60035492 Msa1299040:three_prime_UTR 45.0%
AAGGCTTTCACAGCAACTCT+CGG - chr8_3:60035507-60035526 Msa1299040:three_prime_UTR 45.0%
! ATTGAGCTTTGTGCCAAGCA+AGG + chr8_3:60035584-60035603 Msa1299030:intron 45.0%
GAGAGAGAGAAAGAGGGTTA+CGG + chr8_3:60035607-60035626 Msa1299030:intron 45.0%
GAGAGAGAGAGAGAGAAAGA+GGG + chr8_3:60035613-60035632 Msa1299030:intron 45.0%
AGAGAGAGAGAGAGAGAAAG+AGG + chr8_3:60035614-60035633 Msa1299030:intron 45.0%
TGGCAGTGTGTTTATAAGCG+GGG + chr8_3:60036073-60036092 Msa1299030:CDS 45.0%
CTGGCAGTGTGTTTATAAGC+GGG + chr8_3:60036074-60036093 Msa1299030:CDS 45.0%
GCTGGCAGTGTGTTTATAAG+CGG + chr8_3:60036075-60036094 Msa1299030:CDS 45.0%
TATCCACACTCTAGATGTCC+AGG + chr8_3:60036143-60036162 Msa1299030:CDS 45.0%
GTGTGGATATAAACGGTGGA+TGG - chr8_3:60036154-60036173 Msa1299040:CDS 45.0%
TCGTGTGACTGAATAGCACT+AGG - chr8_3:60036250-60036269 Msa1299040:intron 45.0%
GTGACTGAATAGCACTAGGA+AGG - chr8_3:60036254-60036273 Msa1299040:intron 45.0%
TGGTCTTCATGTCTCTGTGT+TGG - chr8_3:60036608-60036627 Msa1299040:intron 45.0%
GGTCTTCATGTCTCTGTGTT+GGG - chr8_3:60036609-60036628 Msa1299040:intron 45.0%
! ATTATGACAGAGGGGTATGG+GGG + chr8_3:60036664-60036683 None:intergenic 45.0%
GGCTACGTCTATTATGACAG+AGG + chr8_3:60036674-60036693 None:intergenic 45.0%
TCACGTGACTAGGTGATGAT+GGG + chr8_3:60036695-60036714 None:intergenic 45.0%
TTCACGTGACTAGGTGATGA+TGG + chr8_3:60036696-60036715 None:intergenic 45.0%
CACATGTCTTTCACGTGACT+AGG + chr8_3:60036705-60036724 None:intergenic 45.0%
GTCACGTGAAAGACATGTGA+TGG - chr8_3:60036706-60036725 Msa1299040:intron 45.0%
!! TTAAGTTGGGACAGACGAGT+TGG - chr8_3:60036730-60036749 Msa1299040:intron 45.0%
GATATTCATTTCGGGAGCGA+AGG - chr8_3:60036780-60036799 Msa1299040:intron 45.0%
TCCTTTATGGGTCATGACAG+GGG + chr8_3:60036810-60036829 None:intergenic 45.0%
CTCCTTTATGGGTCATGACA+GGG + chr8_3:60036811-60036830 None:intergenic 45.0%
TGTCATGACCCATAAAGGAG+CGG - chr8_3:60036811-60036830 Msa1299040:intron 45.0%
GGAAAACATGTGCTTCTGAC+TGG - chr8_3:60036898-60036917 Msa1299040:intron 45.0%
TCTCAAGAGGATAAGCACGA+AGG - chr8_3:60036922-60036941 Msa1299040:intron 45.0%
CTCAAGAGGATAAGCACGAA+GGG - chr8_3:60036923-60036942 Msa1299040:intron 45.0%
TCAAGAGGATAAGCACGAAG+GGG - chr8_3:60036924-60036943 Msa1299040:intron 45.0%
TACAATGGTATAGACCCTGC+CGG + chr8_3:60037134-60037153 None:intergenic 45.0%
TGTACTTGCGGTGAGTACAA+TGG + chr8_3:60037149-60037168 None:intergenic 45.0%
AAACTTGATGTGGAGGATGC+TGG - chr8_3:60037282-60037301 Msa1299040:intron 45.0%
GCATGTGGTATACAGTAGAG+TGG - chr8_3:60037557-60037576 Msa1299040:CDS 45.0%
AATCCTCACCTCTCGTAAGC+CGG + chr8_3:60036048-60036067 Msa1299030:CDS 50.0%
GGCAGTGTGTTTATAAGCGG+GGG + chr8_3:60036072-60036091 Msa1299030:CDS 50.0%
TGCCAGCCCATATCTTATCC+AGG - chr8_3:60036088-60036107 Msa1299040:CDS 50.0%
GTCCTGGATAAGATATGGGC+TGG + chr8_3:60036093-60036112 Msa1299030:CDS 50.0%
! GGGGGTGTGTTAAATAGTCC+TGG + chr8_3:60036109-60036128 Msa1299030:CDS 50.0%
ATAAACGGTGGATGGCCTAG+TGG - chr8_3:60036162-60036181 Msa1299040:intron 50.0%
CCTTTATGGGTCATGACAGG+GGG + chr8_3:60036809-60036828 None:intergenic 50.0%
GCTCCTTTATGGGTCATGAC+AGG + chr8_3:60036812-60036831 None:intergenic 50.0%
ACTAGGCTCCGCTCCTTTAT+GGG + chr8_3:60036822-60036841 None:intergenic 50.0%
TATCTCGGATACGGGTCACT+AGG + chr8_3:60036839-60036858 None:intergenic 50.0%
!! CTTTTGACTCAGTCCCCACA+TGG - chr8_3:60036877-60036896 Msa1299040:intron 50.0%
ATTCCGCTCTTCCAGGAATG+TGG + chr8_3:60036968-60036987 None:intergenic 50.0%
ATTCCTGGAAGAGCGGAATC+TGG - chr8_3:60036969-60036988 Msa1299040:intron 50.0%
! ATGCCTAGTGAGAAGCGCAA+CGG + chr8_3:60037023-60037042 None:intergenic 50.0%
AATCCTCACCTCTCGTAAGC+CGG + chr8_3:60036048-60036067 Msa1299030:CDS 50.0%
GGCAGTGTGTTTATAAGCGG+GGG + chr8_3:60036072-60036091 Msa1299030:CDS 50.0%
TGCCAGCCCATATCTTATCC+AGG - chr8_3:60036088-60036107 Msa1299040:CDS 50.0%
GTCCTGGATAAGATATGGGC+TGG + chr8_3:60036093-60036112 Msa1299030:CDS 50.0%
! GGGGGTGTGTTAAATAGTCC+TGG + chr8_3:60036109-60036128 Msa1299030:CDS 50.0%
ATAAACGGTGGATGGCCTAG+TGG - chr8_3:60036162-60036181 Msa1299040:intron 50.0%
CCTTTATGGGTCATGACAGG+GGG + chr8_3:60036809-60036828 None:intergenic 50.0%
GCTCCTTTATGGGTCATGAC+AGG + chr8_3:60036812-60036831 None:intergenic 50.0%
ACTAGGCTCCGCTCCTTTAT+GGG + chr8_3:60036822-60036841 None:intergenic 50.0%
TATCTCGGATACGGGTCACT+AGG + chr8_3:60036839-60036858 None:intergenic 50.0%
!! CTTTTGACTCAGTCCCCACA+TGG - chr8_3:60036877-60036896 Msa1299040:intron 50.0%
ATTCCGCTCTTCCAGGAATG+TGG + chr8_3:60036968-60036987 None:intergenic 50.0%
ATTCCTGGAAGAGCGGAATC+TGG - chr8_3:60036969-60036988 Msa1299040:intron 50.0%
! ATGCCTAGTGAGAAGCGCAA+CGG + chr8_3:60037023-60037042 None:intergenic 50.0%
! TTCGTAAGGGTGTGTGAGCG+TGG - chr8_3:60035414-60035433 Msa1299040:three_prime_UTR 55.0%
!! TAACGGAGAAAGTGCCGGTG+AGG + chr8_3:60035548-60035567 Msa1299030:intron 55.0%
! GCACAAAACCGGCTTACGAG+AGG - chr8_3:60036037-60036056 Msa1299040:intron 55.0%
! AAACCGGCTTACGAGAGGTG+AGG - chr8_3:60036042-60036061 Msa1299040:intron 55.0%
TAACACACCCCCTCACGTTC+AGG - chr8_3:60036117-60036136 Msa1299040:CDS 55.0%
ATGTCCAGGCCTGAACGTGA+GGG + chr8_3:60036129-60036148 Msa1299030:CDS 55.0%
! AGGCCTGGACATCTAGAGTG+TGG - chr8_3:60036137-60036156 Msa1299040:CDS 55.0%
!! GTTGGGACAGACGAGTTGGT+TGG - chr8_3:60036734-60036753 Msa1299040:intron 55.0%
TTGGCCACATGCTCGACATG+TGG - chr8_3:60036753-60036772 Msa1299040:intron 55.0%
TCGTCCACATGTCGAGCATG+TGG + chr8_3:60036760-60036779 None:intergenic 55.0%
CCCCCTGTCATGACCCATAA+AGG - chr8_3:60036806-60036825 Msa1299040:intron 55.0%
CACTAGGCTCCGCTCCTTTA+TGG + chr8_3:60036823-60036842 None:intergenic 55.0%
GCTTCTGACTGGGTCTCAAG+AGG - chr8_3:60036909-60036928 Msa1299040:intron 55.0%
AGAGGATAAGCACGAAGGGG+TGG - chr8_3:60036927-60036946 Msa1299040:intron 55.0%
GAGGATAAGCACGAAGGGGT+GGG - chr8_3:60036928-60036947 Msa1299040:intron 55.0%
AGCACGAAGGGGTGGGATAT+CGG - chr8_3:60036935-60036954 Msa1299040:intron 55.0%
AGGGGTGGGATATCGGATGA+AGG - chr8_3:60036942-60036961 Msa1299040:intron 55.0%
TCGGATGAAGGCCACATTCC+TGG - chr8_3:60036954-60036973 Msa1299040:intron 55.0%
AGGCCACATTCCTGGAAGAG+CGG - chr8_3:60036962-60036981 Msa1299040:intron 55.0%
CTACCAGATTCCGCTCTTCC+AGG + chr8_3:60036975-60036994 None:intergenic 55.0%
! GTGAGAAGCGCAACGGCTTT+AGG + chr8_3:60037016-60037035 None:intergenic 55.0%
AAGCCGTTGCGCTTCTCACT+AGG - chr8_3:60037017-60037036 Msa1299040:intron 55.0%
! CCTCTAGCTTGGTACATCGC+CGG - chr8_3:60037112-60037131 Msa1299040:intron 55.0%
TGTGGAGGATGCTGGTGAGA+AGG - chr8_3:60037290-60037309 Msa1299040:intron 55.0%
ACGCTCACACACCCTTACGA+AGG + chr8_3:60035415-60035434 Msa1299030:intron 55.0%
! TTCGTAAGGGTGTGTGAGCG+TGG - chr8_3:60035414-60035433 Msa1299040:three_prime_UTR 55.0%
!! TAACGGAGAAAGTGCCGGTG+AGG + chr8_3:60035548-60035567 Msa1299030:intron 55.0%
! GCACAAAACCGGCTTACGAG+AGG - chr8_3:60036037-60036056 Msa1299040:intron 55.0%
! AAACCGGCTTACGAGAGGTG+AGG - chr8_3:60036042-60036061 Msa1299040:intron 55.0%
TAACACACCCCCTCACGTTC+AGG - chr8_3:60036117-60036136 Msa1299040:CDS 55.0%
ATGTCCAGGCCTGAACGTGA+GGG + chr8_3:60036129-60036148 Msa1299030:CDS 55.0%
! AGGCCTGGACATCTAGAGTG+TGG - chr8_3:60036137-60036156 Msa1299040:CDS 55.0%
!! GTTGGGACAGACGAGTTGGT+TGG - chr8_3:60036734-60036753 Msa1299040:intron 55.0%
TTGGCCACATGCTCGACATG+TGG - chr8_3:60036753-60036772 Msa1299040:intron 55.0%
TCGTCCACATGTCGAGCATG+TGG + chr8_3:60036760-60036779 None:intergenic 55.0%
CCCCCTGTCATGACCCATAA+AGG - chr8_3:60036806-60036825 Msa1299040:intron 55.0%
CACTAGGCTCCGCTCCTTTA+TGG + chr8_3:60036823-60036842 None:intergenic 55.0%
GCTTCTGACTGGGTCTCAAG+AGG - chr8_3:60036909-60036928 Msa1299040:intron 55.0%
AGAGGATAAGCACGAAGGGG+TGG - chr8_3:60036927-60036946 Msa1299040:intron 55.0%
GAGGATAAGCACGAAGGGGT+GGG - chr8_3:60036928-60036947 Msa1299040:intron 55.0%
AGCACGAAGGGGTGGGATAT+CGG - chr8_3:60036935-60036954 Msa1299040:intron 55.0%
AGGGGTGGGATATCGGATGA+AGG - chr8_3:60036942-60036961 Msa1299040:intron 55.0%
TCGGATGAAGGCCACATTCC+TGG - chr8_3:60036954-60036973 Msa1299040:intron 55.0%
AGGCCACATTCCTGGAAGAG+CGG - chr8_3:60036962-60036981 Msa1299040:intron 55.0%
CTACCAGATTCCGCTCTTCC+AGG + chr8_3:60036975-60036994 None:intergenic 55.0%
! GTGAGAAGCGCAACGGCTTT+AGG + chr8_3:60037016-60037035 None:intergenic 55.0%
AAGCCGTTGCGCTTCTCACT+AGG - chr8_3:60037017-60037036 Msa1299040:intron 55.0%
! CCTCTAGCTTGGTACATCGC+CGG - chr8_3:60037112-60037131 Msa1299040:intron 55.0%
TGTGGAGGATGCTGGTGAGA+AGG - chr8_3:60037290-60037309 Msa1299040:intron 55.0%
TGTCCAGGCCTGAACGTGAG+GGG + chr8_3:60036128-60036147 Msa1299030:CDS 60.0%
GATGTCCAGGCCTGAACGTG+AGG + chr8_3:60036130-60036149 Msa1299030:CDS 60.0%
CCGGCGATGTACCAAGCTAG+AGG + chr8_3:60037115-60037134 None:intergenic 60.0%
TAGCTTGGTACATCGCCGGC+AGG - chr8_3:60037116-60037135 Msa1299040:intron 60.0%
AGCTTGGTACATCGCCGGCA+GGG - chr8_3:60037117-60037136 Msa1299040:intron 60.0%
TGTCCAGGCCTGAACGTGAG+GGG + chr8_3:60036128-60036147 Msa1299030:CDS 60.0%
GATGTCCAGGCCTGAACGTG+AGG + chr8_3:60036130-60036149 Msa1299030:CDS 60.0%
CCGGCGATGTACCAAGCTAG+AGG + chr8_3:60037115-60037134 None:intergenic 60.0%
TAGCTTGGTACATCGCCGGC+AGG - chr8_3:60037116-60037135 Msa1299040:intron 60.0%
AGCTTGGTACATCGCCGGCA+GGG - chr8_3:60037117-60037136 Msa1299040:intron 60.0%
GTCCAGGCCTGAACGTGAGG+GGG + chr8_3:60036127-60036146 Msa1299030:CDS 65.0%
GTCCAGGCCTGAACGTGAGG+GGG + chr8_3:60036127-60036146 Msa1299030:CDS 65.0%
CACCCCCTCACGTTCAGGCC+TGG - chr8_3:60036122-60036141 Msa1299040:CDS 70.0%
CACCCCCTCACGTTCAGGCC+TGG - chr8_3:60036122-60036141 Msa1299040:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 60035414 60037650 60035414 ID=Msa1299040;Name=Msa1299040
chr8_3 mRNA 60035414 60037650 60035414 ID=Msa1299040-mRNA-1;Parent=Msa1299040;Name=Msa1299040-mRNA-1;_AED=0.14;_eAED=0.14;_QI=57|0|0.66|0.66|1|1|3|330|91
chr8_3 exon 60035414 60035782 60035414 ID=Msa1299040-mRNA-1:exon:17463;Parent=Msa1299040-mRNA-1
chr8_3 exon 60036074 60036175 60036074 ID=Msa1299040-mRNA-1:exon:17462;Parent=Msa1299040-mRNA-1
chr8_3 exon 60037459 60037650 60037459 ID=Msa1299040-mRNA-1:exon:17461;Parent=Msa1299040-mRNA-1
chr8_3 five_prime_UTR 60037594 60037650 60037594 ID=Msa1299040-mRNA-1:five_prime_utr;Parent=Msa1299040-mRNA-1
chr8_3 CDS 60037459 60037593 60037459 ID=Msa1299040-mRNA-1:cds;Parent=Msa1299040-mRNA-1
chr8_3 CDS 60036074 60036175 60036074 ID=Msa1299040-mRNA-1:cds;Parent=Msa1299040-mRNA-1
chr8_3 CDS 60035744 60035782 60035744 ID=Msa1299040-mRNA-1:cds;Parent=Msa1299040-mRNA-1
chr8_3 three_prime_UTR 60035414 60035743 60035414 ID=Msa1299040-mRNA-1:three_prime_utr;Parent=Msa1299040-mRNA-1
Gene Sequence

>Msa1299040

ATGGGAATAGCTGGTAAGATTGGGGATTACACTTTCAAGGCTTTCACAGCAACTCTCGGCATCACTACTATCTACCTCACCGGCACTTTCTCCGTTAATGTTTACAAAGGCCTTGCTTGGCACAAAGCTCAATCCTCCCCACATGGAAAACATGTGCTTCTGACTGGGTCTCAAGAGGATAAGCACGAAGGGGTGGGATATCGGATGAAGGCCACATTCCTGGAAGAGCGGAATCTGAAACTTGATGTGGAGGATGCTGGTGAGAAGGCTTCGTGA
Protein sequence

>Msa1299040

MGIAGKIGDYTFKAFTATLGITTIYLTGTFSVNVYKGLAWHKAQSSPHGKHVLLTGSQEDKHEGVGYRMKATFLEERNLKLDVEDAGEKAS*