AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1306260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1306260 MtrunA17_Chr5g0393521 39.316 117 47 4 2 94 53 169 9.74e-16 70.1
Msa1306260 MtrunA17_Chr1g0167871 38.462 117 48 4 2 94 500 616 1.04e-15 70.9
Msa1306260 MtrunA17_Chr5g0417421 38.462 117 48 4 2 94 121 237 2.62e-15 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 75 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATGACCTATGGGATTTC+AGG 0.142267 8_4:-1067009 None:intergenic
TTGTTTAGGATGATCAATTT+TGG 0.186172 8_4:+1068134 Msa1306260:three_prime_UTR
TCTTCGCTAACACCAGAAAA+AGG 0.217962 8_4:+1068457 Msa1306260:three_prime_UTR
TCGAGTTGATCCAGCAAAAT+TGG 0.244680 8_4:-1067910 None:intergenic
TTTATGTTCATACGAATTAT+AGG 0.258066 8_4:+1068084 Msa1306260:three_prime_UTR
TACCAGTGATTCTGAACTTC+AGG 0.282604 8_4:+1068279 Msa1306260:three_prime_UTR
AGCAAAATTGGATATTCTAA+TGG 0.286408 8_4:-1067898 None:intergenic
TTCTATTTCTATCTATGTAC+TGG 0.315789 8_4:+1066518 Msa1306260:five_prime_UTR
AGACCAATGTTATCAATCTA+AGG 0.317503 8_4:+1067579 Msa1306260:CDS
TTATGTTCATACGAATTATA+GGG 0.319659 8_4:+1068085 Msa1306260:three_prime_UTR
TAGTATCAACCATTCTTTGA+TGG 0.327014 8_4:-1067478 None:intergenic
AAGCATGTGTCAAATGTTAT+AGG 0.330903 8_4:+1067510 Msa1306260:CDS
ATCCTAAACAAATAGCAAAA+TGG 0.349955 8_4:-1068122 None:intergenic
AACTCACTTCAGCTTGAGTA+TGG 0.353110 8_4:-1064932 None:intergenic
ACAAAGAACAATGCTGAATC+TGG 0.353144 8_4:+1066597 Msa1306260:five_prime_UTR
AGTTGCATTATTTCATTTAG+TGG 0.354080 8_4:-1067272 None:intergenic
TAACTTTGTTGAAGATTGTT+TGG 0.360834 8_4:-1068532 None:intergenic
CGTCACACGCGGTCATTGAA+TGG 0.360842 8_4:-1065351 None:intergenic
AAAGAAAGTTCACTACTTCA+TGG 0.370738 8_4:+1065665 Msa1306260:five_prime_UTR
TGGGACAGTTGTTCTGTTCA+AGG 0.400212 8_4:+1067970 Msa1306260:CDS
ACTATAGAAACAAAAGAGAT+TGG 0.417735 8_4:-1066084 None:intergenic
TGTTCTTATCCATGTGTTCA+AGG 0.427610 8_4:+1065855 Msa1306260:five_prime_UTR
AACAATCTTCAACAAAGTTA+AGG 0.430707 8_4:+1068535 Msa1306260:three_prime_UTR
AGCATGTGTCAAATGTTATA+GGG 0.439555 8_4:+1067511 Msa1306260:CDS
AAGTTAAGTGGCAGCCAACT+TGG 0.439945 8_4:+1067950 Msa1306260:CDS
GAAATGTTTGTAAAGTTAAG+TGG 0.449292 8_4:+1067938 Msa1306260:CDS
AGTTTAGTCTTATGTTACTT+TGG 0.456396 8_4:+1068176 Msa1306260:three_prime_UTR
TGGTTATTTATCATAAAAGA+TGG 0.468935 8_4:+1068249 Msa1306260:three_prime_UTR
CGCGTGTGACGAAAATCAAC+AGG 0.474646 8_4:+1065363 Msa1306260:five_prime_UTR
TTCAGAATCACTGGTAATGC+TGG 0.482229 8_4:-1068272 None:intergenic
GAGATGGAAGTGGAATATAA+TGG 0.485881 8_4:+1067698 Msa1306260:CDS
CAAATGTTATAGGGCGGCAA+AGG 0.495945 8_4:+1067520 Msa1306260:CDS
GATGATGAAGATGGGCTCAC+TGG 0.503503 8_4:-1067197 None:intergenic
ACCAGTGATTCTGAACTTCA+GGG 0.504024 8_4:+1068280 Msa1306260:three_prime_UTR
GTATGGAAGAGAGAGAGGCT+TGG 0.504350 8_4:-1064915 None:intergenic
TGATGTGATTCTTATGTTGA+AGG 0.505243 8_4:+1066223 Msa1306260:five_prime_UTR
TTGTAGGTTTAAGTTAGAGA+TGG 0.513043 8_4:+1067682 Msa1306260:intron
CTATAGAAACAAAAGAGATT+GGG 0.515222 8_4:-1066083 None:intergenic
AATTAATCAATCGCCGTATG+TGG 0.516337 8_4:+1068639 Msa1306260:three_prime_UTR
GTTTAAGTTAGAGATGGAAG+TGG 0.518694 8_4:+1067688 Msa1306260:CDS
ACGAACTGTGATATCAGATC+TGG 0.519162 8_4:+1065165 Msa1306260:five_prime_UTR
ACCCTGAAGTTCAGAATCAC+TGG 0.520945 8_4:-1068281 None:intergenic
TTCGTTGCAACTACTTCAAG+AGG 0.523500 8_4:-1065147 None:intergenic
TCTATTTCTATCTATGTACT+GGG 0.524926 8_4:+1066519 Msa1306260:five_prime_UTR
AAAGTGAAACCATCAAAGAA+TGG 0.537737 8_4:+1067469 Msa1306260:exon
TAGTGATTAGAATAAACAAA+AGG 0.539249 8_4:-1066465 None:intergenic
AAAGAAATACACAATATGAA+TGG 0.543270 8_4:-1066303 None:intergenic
AAATGTTATAGGGCGGCAAA+GGG 0.559414 8_4:+1067521 Msa1306260:CDS
AACATGGGATCTCTAATGCA+TGG 0.560922 8_4:+1066962 Msa1306260:five_prime_UTR
GAGAATGATGAGTCTTCGAC+AGG 0.561049 8_4:-1067143 None:intergenic
CTTGAGTATGGAAGAGAGAG+AGG 0.561484 8_4:-1064920 None:intergenic
TGTGACAGAGATGATGAAGA+TGG 0.565680 8_4:-1067206 None:intergenic
AGTTAAGTGGCAGCCAACTT+GGG 0.566681 8_4:+1067951 Msa1306260:CDS
GTTCTGTTCAAGGAGGATGA+AGG 0.568696 8_4:+1067980 Msa1306260:CDS
ACCATTGTGAAGAAGAATGA+TGG 0.575193 8_4:-1066625 None:intergenic
TATCAATCCTGAAATCCCAT+AGG 0.579861 8_4:+1067002 Msa1306260:five_prime_UTR
ATGATGAAGATGGGCTCACT+GGG 0.582220 8_4:-1067196 None:intergenic
TATCTTCAACCTTGAACACA+TGG 0.591729 8_4:-1065864 None:intergenic
ATGGTGAAGATAGAAGATAA+AGG 0.593300 8_4:+1065684 Msa1306260:five_prime_UTR
AAATGAAATAATGCAACTCA+CGG 0.594077 8_4:+1067277 Msa1306260:five_prime_UTR
AGAGTAAATAGGGCCACATA+CGG 0.595559 8_4:-1068652 None:intergenic
TTTATAGAGAAGTATGAACA+TGG 0.603096 8_4:+1066946 Msa1306260:intron
ACAGAACAACTGTCCCAAGT+TGG 0.613921 8_4:-1067964 None:intergenic
AAACCTTAGATTGATAACAT+TGG 0.621112 8_4:-1067582 None:intergenic
TCACAGAAAGCAGACAGTGT+TGG 0.629389 8_4:-1068415 None:intergenic
ACCATCATTCTTCTTCACAA+TGG 0.635895 8_4:+1066624 Msa1306260:five_prime_UTR
GTGACAGAGATGATGAAGAT+GGG 0.640666 8_4:-1067205 None:intergenic
CTATTTCTATCTATGTACTG+GGG 0.649914 8_4:+1066520 Msa1306260:five_prime_UTR
GACAGTTGTTCTGTTCAAGG+AGG 0.654985 8_4:+1067973 Msa1306260:CDS
TGAACAAACACACTTTATCG+TGG 0.656999 8_4:-1066407 None:intergenic
TTATAGAGAAGTATGAACAT+GGG 0.666382 8_4:+1066947 Msa1306260:intron
ATGTGTCAAATGTTATAGGG+CGG 0.686190 8_4:+1067514 Msa1306260:CDS
TGAAATAATGCAACTCACGG+AGG 0.707979 8_4:+1067280 Msa1306260:five_prime_UTR
TTGTGAAGAAGAATGATGGT+TGG 0.724119 8_4:-1066621 None:intergenic
GCATGTTTGAAAATATGACA+TGG 0.728799 8_4:+1068229 Msa1306260:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGCAAAAAAAAAAAAAAAAA+AGG - chr8_4:1065408-1065427 None:intergenic 10.0%
!! TGAAAAAAAAATTGATGAAA+TGG - chr8_4:1065509-1065528 None:intergenic 15.0%
!! AATAAAAATAAGGAATTGAA+TGG - chr8_4:1065770-1065789 None:intergenic 15.0%
!! AGCATACAAAAATAAAAATA+AGG - chr8_4:1065780-1065799 None:intergenic 15.0%
!!! ATTTTTTTTCAATCAAAAGA+AGG - chr8_4:1066342-1066361 None:intergenic 15.0%
!! TGTTATATTATTTATAGATC+AGG - chr8_4:1066708-1066727 None:intergenic 15.0%
!! TTAGAAAGATGTTATAATTA+CGG - chr8_4:1067845-1067864 None:intergenic 15.0%
!! AAAAAATAGTAGAGTAAATA+GGG - chr8_4:1068665-1068684 None:intergenic 15.0%
!! ATAAAGAAAGAAAAAGTAGT+GGG - chr8_4:1064961-1064980 None:intergenic 20.0%
!! AATAAAGAAAGAAAAAGTAG+TGG - chr8_4:1064962-1064981 None:intergenic 20.0%
!! AAAGAAATCTAAAGAAAGAA+AGG - chr8_4:1064993-1065012 None:intergenic 20.0%
!!! AGATTTCTTTTTAAAAGCTT+TGG + chr8_4:1065003-1065022 Msa1306260:intron 20.0%
!! TAGAAAAGGGAAAAAAATAT+TGG - chr8_4:1065199-1065218 None:intergenic 20.0%
!! TGCATCAATAATATAGAAAA+GGG - chr8_4:1065212-1065231 None:intergenic 20.0%
!! ATGCATCAATAATATAGAAA+AGG - chr8_4:1065213-1065232 None:intergenic 20.0%
!! TATTATTGATGCATATTTGT+TGG + chr8_4:1065219-1065238 Msa1306260:intron 20.0%
!! TCTATAGTGCAAAAAAAAAA+AGG + chr8_4:1066099-1066118 Msa1306260:five_prime_UTR 20.0%
!!! TTAATTTTGTAAGTCAATCA+TGG - chr8_4:1066164-1066183 None:intergenic 20.0%
!! AAAGAAATACACAATATGAA+TGG - chr8_4:1066306-1066325 None:intergenic 20.0%
!!! TTTTTTCTATTGCAATTTGT+TGG - chr8_4:1066378-1066397 None:intergenic 20.0%
!! TAGTGATTAGAATAAACAAA+AGG - chr8_4:1066468-1066487 None:intergenic 20.0%
!! CATAGTAAAAAATAATTGTG+AGG - chr8_4:1066864-1066883 None:intergenic 20.0%
!! AAAAATTGTAGTAAACAAAC+AGG - chr8_4:1067105-1067124 None:intergenic 20.0%
!!! CAATTTTTGCAAATGTATTT+AGG + chr8_4:1067117-1067136 Msa1306260:intron 20.0%
!!! TAGCCAAGTTTTTTTTTTTT+GGG + chr8_4:1067726-1067745 Msa1306260:CDS 20.0%
!! TTTATGTTCATACGAATTAT+AGG + chr8_4:1068084-1068103 Msa1306260:three_prime_UTR 20.0%
!! TTATGTTCATACGAATTATA+GGG + chr8_4:1068085-1068104 Msa1306260:three_prime_UTR 20.0%
!! TGGTTATTTATCATAAAAGA+TGG + chr8_4:1068249-1068268 Msa1306260:three_prime_UTR 20.0%
!! TCAGGTTATATAGTTTATTT+AGG + chr8_4:1068338-1068357 Msa1306260:intron 20.0%
!! CTATAGTCTAAAAACTAAAA+TGG - chr8_4:1068608-1068627 None:intergenic 20.0%
!! CAAAAAATAGTAGAGTAAAT+AGG - chr8_4:1068666-1068685 None:intergenic 20.0%
!! AAGCTTTTTATTAGTCAAAG+CGG + chr8_4:1065122-1065141 Msa1306260:five_prime_UTR 25.0%
!!! ATAGTTGTTTTAGAGCAAAA+GGG - chr8_4:1065280-1065299 None:intergenic 25.0%
!!! AATAGTTGTTTTAGAGCAAA+AGG - chr8_4:1065281-1065300 None:intergenic 25.0%
!!! GATTAAAGTTTTGTTGTTGT+TGG + chr8_4:1065533-1065552 Msa1306260:intron 25.0%
! TTTCTACGATGCAAAAAAAA+AGG + chr8_4:1065915-1065934 Msa1306260:five_prime_UTR 25.0%
! TCAACAAATTCAGAAATGAA+AGG - chr8_4:1065956-1065975 None:intergenic 25.0%
!!! TGTTGTGATTCTTATTTTGA+AGG + chr8_4:1066037-1066056 Msa1306260:intron 25.0%
!!! ATTCTTATTTTGAAGGTTGA+AGG + chr8_4:1066044-1066063 Msa1306260:intron 25.0%
! CTATAGAAACAAAAGAGATT+GGG - chr8_4:1066086-1066105 None:intergenic 25.0%
! ACTATAGAAACAAAAGAGAT+TGG - chr8_4:1066087-1066106 None:intergenic 25.0%
!! TTTTGACATGATTTAGATCA+AGG + chr8_4:1066270-1066289 Msa1306260:five_prime_UTR 25.0%
!!! TTCTATTTCTATCTATGTAC+TGG + chr8_4:1066518-1066537 Msa1306260:five_prime_UTR 25.0%
!!! TCTATTTCTATCTATGTACT+GGG + chr8_4:1066519-1066538 Msa1306260:five_prime_UTR 25.0%
! TTATTTATAGATCAGGTATG+TGG - chr8_4:1066701-1066720 None:intergenic 25.0%
!! TTTATAGAGAAGTATGAACA+TGG + chr8_4:1066946-1066965 Msa1306260:intron 25.0%
!! TTATAGAGAAGTATGAACAT+GGG + chr8_4:1066947-1066966 Msa1306260:intron 25.0%
!! TTTTGAATTCTATGACCTAT+GGG - chr8_4:1067020-1067039 None:intergenic 25.0%
!! TTTTTGAATTCTATGACCTA+TGG - chr8_4:1067021-1067040 None:intergenic 25.0%
! TAGGTCATAGAATTCAAAAA+AGG + chr8_4:1067021-1067040 Msa1306260:five_prime_UTR 25.0%
! AGGTCATAGAATTCAAAAAA+GGG + chr8_4:1067022-1067041 Msa1306260:five_prime_UTR 25.0%
! AGTTGCATTATTTCATTTAG+TGG - chr8_4:1067275-1067294 None:intergenic 25.0%
! AAATGAAATAATGCAACTCA+CGG + chr8_4:1067277-1067296 Msa1306260:five_prime_UTR 25.0%
!! AAACCTTAGATTGATAACAT+TGG - chr8_4:1067585-1067604 None:intergenic 25.0%
! AACTTAAACCTACAATACAT+GGG - chr8_4:1067677-1067696 None:intergenic 25.0%
! TAACTTAAACCTACAATACA+TGG - chr8_4:1067678-1067697 None:intergenic 25.0%
!!! GTAGCCAAGTTTTTTTTTTT+TGG + chr8_4:1067725-1067744 Msa1306260:CDS 25.0%
! GATCCCAAAAAAAAAAAACT+TGG - chr8_4:1067732-1067751 None:intergenic 25.0%
! TACGGAAGTTTAACATAATA+AGG - chr8_4:1067827-1067846 None:intergenic 25.0%
! AGCAAAATTGGATATTCTAA+TGG - chr8_4:1067901-1067920 None:intergenic 25.0%
!! ATTAGAATATCCAATTTTGC+TGG + chr8_4:1067900-1067919 Msa1306260:CDS 25.0%
! GAAATGTTTGTAAAGTTAAG+TGG + chr8_4:1067938-1067957 Msa1306260:CDS 25.0%
! ATCCTAAACAAATAGCAAAA+TGG - chr8_4:1068125-1068144 None:intergenic 25.0%
!!! TTGTTTAGGATGATCAATTT+TGG + chr8_4:1068134-1068153 Msa1306260:three_prime_UTR 25.0%
!!! TGTTTAGGATGATCAATTTT+GGG + chr8_4:1068135-1068154 Msa1306260:three_prime_UTR 25.0%
!!! AGTTTAGTCTTATGTTACTT+TGG + chr8_4:1068176-1068195 Msa1306260:three_prime_UTR 25.0%
! TAACTTTGTTGAAGATTGTT+TGG - chr8_4:1068535-1068554 None:intergenic 25.0%
! AACAATCTTCAACAAAGTTA+AGG + chr8_4:1068535-1068554 Msa1306260:three_prime_UTR 25.0%
TTAGTCATAGTTATGAGATC+TGG - chr8_4:1065098-1065117 None:intergenic 30.0%
!! TGTACTAAGTGTTTTTGTTG+TGG + chr8_4:1065252-1065271 Msa1306260:intron 30.0%
!! TGCTCTAAAACAACTATTGT+TGG + chr8_4:1065283-1065302 Msa1306260:intron 30.0%
GAGTTTGACATGAAAACAAA+AGG - chr8_4:1065474-1065493 None:intergenic 30.0%
AAAGAAAGTTCACTACTTCA+TGG + chr8_4:1065665-1065684 Msa1306260:five_prime_UTR 30.0%
ATGGTGAAGATAGAAGATAA+AGG + chr8_4:1065684-1065703 Msa1306260:five_prime_UTR 30.0%
! AAGGAATTGAATGGTTAGTT+CGG - chr8_4:1065761-1065780 None:intergenic 30.0%
TGATGTGATTCTTATGTTGA+AGG + chr8_4:1066223-1066242 Msa1306260:five_prime_UTR 30.0%
!! CTATTTCTATCTATGTACTG+GGG + chr8_4:1066520-1066539 Msa1306260:five_prime_UTR 30.0%
TCTTCACAATGGTAAGATTA+AGG + chr8_4:1066635-1066654 Msa1306260:intron 30.0%
TGTGACTTATTAGTAACATG+TGG - chr8_4:1066664-1066683 None:intergenic 30.0%
!! TCGTCTTTTGAGAGATTTAT+TGG + chr8_4:1067233-1067252 Msa1306260:five_prime_UTR 30.0%
TGTTCAGGTCATAATAATGT+AGG - chr8_4:1067385-1067404 None:intergenic 30.0%
AAAGTGAAACCATCAAAGAA+TGG + chr8_4:1067469-1067488 Msa1306260:exon 30.0%
TAGTATCAACCATTCTTTGA+TGG - chr8_4:1067481-1067500 None:intergenic 30.0%
AAGCATGTGTCAAATGTTAT+AGG + chr8_4:1067510-1067529 Msa1306260:CDS 30.0%
AGCATGTGTCAAATGTTATA+GGG + chr8_4:1067511-1067530 Msa1306260:CDS 30.0%
AGACCAATGTTATCAATCTA+AGG + chr8_4:1067579-1067598 Msa1306260:CDS 30.0%
ATTTACTACCCATGTATTGT+AGG + chr8_4:1067666-1067685 Msa1306260:intron 30.0%
! TTGTAGGTTTAAGTTAGAGA+TGG + chr8_4:1067682-1067701 Msa1306260:intron 30.0%
AGAATTGTTCCGAAAAATTC+AGG + chr8_4:1068022-1068041 Msa1306260:three_prime_UTR 30.0%
! ATAACCTGACCTGAATTTTT+CGG - chr8_4:1068034-1068053 None:intergenic 30.0%
!!! CACCATTTTGCTATTTGTTT+AGG + chr8_4:1068120-1068139 Msa1306260:three_prime_UTR 30.0%
GCATGTTTGAAAATATGACA+TGG + chr8_4:1068229-1068248 Msa1306260:three_prime_UTR 30.0%
CTGTATGTTTCTTTAGATTC+AGG + chr8_4:1068320-1068339 Msa1306260:intron 30.0%
ACCAAAAACAACATTGTTTG+GGG - chr8_4:1068714-1068733 None:intergenic 30.0%
GACCAAAAACAACATTGTTT+GGG - chr8_4:1068715-1068734 None:intergenic 30.0%
AGACCAAAAACAACATTGTT+TGG - chr8_4:1068716-1068735 None:intergenic 30.0%
AGAACAACATAGATCTGACA+TGG - chr8_4:1065841-1065860 None:intergenic 35.0%
TGTTCTTATCCATGTGTTCA+AGG + chr8_4:1065855-1065874 Msa1306260:five_prime_UTR 35.0%
TATCTTCAACCTTGAACACA+TGG - chr8_4:1065867-1065886 None:intergenic 35.0%
TGAACAAACACACTTTATCG+TGG - chr8_4:1066410-1066429 None:intergenic 35.0%
ACAAAGAACAATGCTGAATC+TGG + chr8_4:1066597-1066616 Msa1306260:five_prime_UTR 35.0%
TTGTGAAGAAGAATGATGGT+TGG - chr8_4:1066624-1066643 None:intergenic 35.0%
ACCATCATTCTTCTTCACAA+TGG + chr8_4:1066624-1066643 Msa1306260:five_prime_UTR 35.0%
ACCATTGTGAAGAAGAATGA+TGG - chr8_4:1066628-1066647 None:intergenic 35.0%
ATGCACGGGGAATTATAAAT+TGG - chr8_4:1066837-1066856 None:intergenic 35.0%
TATCAATCCTGAAATCCCAT+AGG + chr8_4:1067002-1067021 Msa1306260:five_prime_UTR 35.0%
ACATACTTGGCTTATGATCT+TGG - chr8_4:1067076-1067095 None:intergenic 35.0%
AAACAGGATTGCAACATACT+TGG - chr8_4:1067089-1067108 None:intergenic 35.0%
GCAACAAATCAGAAATGTTC+AGG - chr8_4:1067400-1067419 None:intergenic 35.0%
ATGTGTCAAATGTTATAGGG+CGG + chr8_4:1067514-1067533 Msa1306260:CDS 35.0%
CATGCATGTTGACAAGATAT+TGG + chr8_4:1067621-1067640 Msa1306260:intron 35.0%
! GTTTAAGTTAGAGATGGAAG+TGG + chr8_4:1067688-1067707 Msa1306260:CDS 35.0%
GAGATGGAAGTGGAATATAA+TGG + chr8_4:1067698-1067717 Msa1306260:CDS 35.0%
CTAACAAATGCACTGCATTT+AGG + chr8_4:1067861-1067880 Msa1306260:intron 35.0%
!!! ATTTTGGGTGTTGAAGATGT+TGG + chr8_4:1068150-1068169 Msa1306260:three_prime_UTR 35.0%
AATTAATCAATCGCCGTATG+TGG + chr8_4:1068639-1068658 Msa1306260:three_prime_UTR 35.0%
!!! ACCCCAAACAATGTTGTTTT+TGG + chr8_4:1068710-1068729 Msa1306260:three_prime_UTR 35.0%
AACTCACTTCAGCTTGAGTA+TGG - chr8_4:1064935-1064954 None:intergenic 40.0%
TTCGTTGCAACTACTTCAAG+AGG - chr8_4:1065150-1065169 None:intergenic 40.0%
ACGAACTGTGATATCAGATC+TGG + chr8_4:1065165-1065184 Msa1306260:five_prime_UTR 40.0%
!!! TACTGCTGCTTGCTCTTTTT+TGG + chr8_4:1065629-1065648 Msa1306260:intron 40.0%
TAATTGTGAGGTAGCATGCA+CGG - chr8_4:1066852-1066871 None:intergenic 40.0%
AACATGGGATCTCTAATGCA+TGG + chr8_4:1066962-1066981 Msa1306260:five_prime_UTR 40.0%
TCTATGACCTATGGGATTTC+AGG - chr8_4:1067012-1067031 None:intergenic 40.0%
GTGACAGAGATGATGAAGAT+GGG - chr8_4:1067208-1067227 None:intergenic 40.0%
TGTGACAGAGATGATGAAGA+TGG - chr8_4:1067209-1067228 None:intergenic 40.0%
TGAAATAATGCAACTCACGG+AGG + chr8_4:1067280-1067299 Msa1306260:five_prime_UTR 40.0%
AAATGTTATAGGGCGGCAAA+GGG + chr8_4:1067521-1067540 Msa1306260:CDS 40.0%
!! ACATTGGTCTCATGCTTTTC+AGG - chr8_4:1067569-1067588 None:intergenic 40.0%
!! TCGAGTTGATCCAGCAAAAT+TGG - chr8_4:1067913-1067932 None:intergenic 40.0%
TGTTCCGAAAAATTCAGGTC+AGG + chr8_4:1068027-1068046 Msa1306260:three_prime_UTR 40.0%
! TTCAGAATCACTGGTAATGC+TGG - chr8_4:1068275-1068294 None:intergenic 40.0%
TACCAGTGATTCTGAACTTC+AGG + chr8_4:1068279-1068298 Msa1306260:three_prime_UTR 40.0%
ACCAGTGATTCTGAACTTCA+GGG + chr8_4:1068280-1068299 Msa1306260:three_prime_UTR 40.0%
TCTTCGCTAACACCAGAAAA+AGG + chr8_4:1068457-1068476 Msa1306260:three_prime_UTR 40.0%
CTTCGCTAACACCAGAAAAA+GGG + chr8_4:1068458-1068477 Msa1306260:three_prime_UTR 40.0%
! AGAATCTGCAACCCTTTTTC+TGG - chr8_4:1068472-1068491 None:intergenic 40.0%
AGAGTAAATAGGGCCACATA+CGG - chr8_4:1068655-1068674 None:intergenic 40.0%
CTTGAGTATGGAAGAGAGAG+AGG - chr8_4:1064923-1064942 None:intergenic 45.0%
!!! CTGTTGATTTTCGTCACACG+CGG - chr8_4:1065365-1065384 None:intergenic 45.0%
AATTGTGAGGTAGCATGCAC+GGG - chr8_4:1066851-1066870 None:intergenic 45.0%
GAGAATGATGAGTCTTCGAC+AGG - chr8_4:1067146-1067165 None:intergenic 45.0%
! ATGATGAAGATGGGCTCACT+GGG - chr8_4:1067199-1067218 None:intergenic 45.0%
CAAATGTTATAGGGCGGCAA+AGG + chr8_4:1067520-1067539 Msa1306260:CDS 45.0%
!! TGCTTTTCAGGACACAACAG+AGG - chr8_4:1067557-1067576 None:intergenic 45.0%
CGTGAGTGAAATGAGTTGCT+TGG + chr8_4:1067751-1067770 Msa1306260:intron 45.0%
TACAACTGCAGACCTTCACA+GGG + chr8_4:1067777-1067796 Msa1306260:intron 45.0%
CAAATGCACTGCATTTAGGC+TGG + chr8_4:1067865-1067884 Msa1306260:intron 45.0%
AAGTTAAGTGGCAGCCAACT+TGG + chr8_4:1067950-1067969 Msa1306260:CDS 45.0%
AGTTAAGTGGCAGCCAACTT+GGG + chr8_4:1067951-1067970 Msa1306260:CDS 45.0%
ACAGAACAACTGTCCCAAGT+TGG - chr8_4:1067967-1067986 None:intergenic 45.0%
TGGGACAGTTGTTCTGTTCA+AGG + chr8_4:1067970-1067989 Msa1306260:CDS 45.0%
GACAGTTGTTCTGTTCAAGG+AGG + chr8_4:1067973-1067992 Msa1306260:CDS 45.0%
GTTCTGTTCAAGGAGGATGA+AGG + chr8_4:1067980-1067999 Msa1306260:CDS 45.0%
ACCCTGAAGTTCAGAATCAC+TGG - chr8_4:1068284-1068303 None:intergenic 45.0%
TCACAGAAAGCAGACAGTGT+TGG - chr8_4:1068418-1068437 None:intergenic 45.0%
!! ATAAAATAATAAGAAATATA+AGG - chr8_4:1066140-1066159 None:intergenic 5.0%
!! AAAGAATTATTAAAATTTAT+AGG - chr8_4:1066764-1066783 None:intergenic 5.0%
GTATGGAAGAGAGAGAGGCT+TGG - chr8_4:1064918-1064937 None:intergenic 50.0%
CGCGTGTGACGAAAATCAAC+AGG + chr8_4:1065363-1065382 Msa1306260:five_prime_UTR 50.0%
ATTGTGAGGTAGCATGCACG+GGG - chr8_4:1066850-1066869 None:intergenic 50.0%
! GATGATGAAGATGGGCTCAC+TGG - chr8_4:1067200-1067219 None:intergenic 50.0%
CTACAACTGCAGACCTTCAC+AGG + chr8_4:1067776-1067795 Msa1306260:intron 50.0%
CGTCACACGCGGTCATTGAA+TGG - chr8_4:1065354-1065373 None:intergenic 55.0%
CCTCAGTCACGACCCTGTGA+AGG - chr8_4:1067792-1067811 None:intergenic 60.0%
CCTTCACAGGGTCGTGACTG+AGG + chr8_4:1067789-1067808 Msa1306260:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 1064903 1068748 1064903 ID=Msa1306260;Name=Msa1306260
chr8_4 mRNA 1064903 1068748 1064903 ID=Msa1306260-mRNA-1;Parent=Msa1306260;Name=Msa1306260-mRNA-1;_AED=0.05;_eAED=0.05;_QI=1178|0.91|0.92|1|0.33|0.23|13|656|97
chr8_4 exon 1064903 1064947 1064903 ID=Msa1306260-mRNA-1:exon:181;Parent=Msa1306260-mRNA-1
chr8_4 exon 1065099 1065186 1065099 ID=Msa1306260-mRNA-1:exon:182;Parent=Msa1306260-mRNA-1
chr8_4 exon 1065321 1065384 1065321 ID=Msa1306260-mRNA-1:exon:183;Parent=Msa1306260-mRNA-1
chr8_4 exon 1065659 1065750 1065659 ID=Msa1306260-mRNA-1:exon:184;Parent=Msa1306260-mRNA-1
chr8_4 exon 1065847 1065936 1065847 ID=Msa1306260-mRNA-1:exon:185;Parent=Msa1306260-mRNA-1
chr8_4 exon 1066059 1066120 1066059 ID=Msa1306260-mRNA-1:exon:186;Parent=Msa1306260-mRNA-1
chr8_4 exon 1066215 1066645 1066215 ID=Msa1306260-mRNA-1:exon:187;Parent=Msa1306260-mRNA-1
chr8_4 exon 1066953 1067043 1066953 ID=Msa1306260-mRNA-1:exon:188;Parent=Msa1306260-mRNA-1
chr8_4 exon 1067139 1067301 1067139 ID=Msa1306260-mRNA-1:exon:189;Parent=Msa1306260-mRNA-1
chr8_4 exon 1067436 1067600 1067436 ID=Msa1306260-mRNA-1:exon:190;Parent=Msa1306260-mRNA-1
chr8_4 exon 1067688 1067751 1067688 ID=Msa1306260-mRNA-1:exon:191;Parent=Msa1306260-mRNA-1
chr8_4 exon 1067883 1068321 1067883 ID=Msa1306260-mRNA-1:exon:192;Parent=Msa1306260-mRNA-1
chr8_4 exon 1068415 1068748 1068415 ID=Msa1306260-mRNA-1:exon:193;Parent=Msa1306260-mRNA-1
chr8_4 five_prime_UTR 1064903 1064947 1064903 ID=Msa1306260-mRNA-1:five_prime_utr;Parent=Msa1306260-mRNA-1
chr8_4 five_prime_UTR 1065099 1065186 1065099 ID=Msa1306260-mRNA-1:five_prime_utr;Parent=Msa1306260-mRNA-1
chr8_4 five_prime_UTR 1065321 1065384 1065321 ID=Msa1306260-mRNA-1:five_prime_utr;Parent=Msa1306260-mRNA-1
chr8_4 five_prime_UTR 1065659 1065750 1065659 ID=Msa1306260-mRNA-1:five_prime_utr;Parent=Msa1306260-mRNA-1
chr8_4 five_prime_UTR 1065847 1065936 1065847 ID=Msa1306260-mRNA-1:five_prime_utr;Parent=Msa1306260-mRNA-1
chr8_4 five_prime_UTR 1066059 1066120 1066059 ID=Msa1306260-mRNA-1:five_prime_utr;Parent=Msa1306260-mRNA-1
chr8_4 five_prime_UTR 1066215 1066645 1066215 ID=Msa1306260-mRNA-1:five_prime_utr;Parent=Msa1306260-mRNA-1
chr8_4 five_prime_UTR 1066953 1067043 1066953 ID=Msa1306260-mRNA-1:five_prime_utr;Parent=Msa1306260-mRNA-1
chr8_4 five_prime_UTR 1067139 1067301 1067139 ID=Msa1306260-mRNA-1:five_prime_utr;Parent=Msa1306260-mRNA-1
chr8_4 five_prime_UTR 1067436 1067487 1067436 ID=Msa1306260-mRNA-1:five_prime_utr;Parent=Msa1306260-mRNA-1
chr8_4 CDS 1067488 1067600 1067488 ID=Msa1306260-mRNA-1:cds;Parent=Msa1306260-mRNA-1
chr8_4 CDS 1067688 1067751 1067688 ID=Msa1306260-mRNA-1:cds;Parent=Msa1306260-mRNA-1
chr8_4 CDS 1067883 1067999 1067883 ID=Msa1306260-mRNA-1:cds;Parent=Msa1306260-mRNA-1
chr8_4 three_prime_UTR 1068000 1068321 1068000 ID=Msa1306260-mRNA-1:three_prime_utr;Parent=Msa1306260-mRNA-1
chr8_4 three_prime_UTR 1068415 1068748 1068415 ID=Msa1306260-mRNA-1:three_prime_utr;Parent=Msa1306260-mRNA-1
Gene Sequence

>Msa1306260

ATGGTTGATACTACAAAGTACAAAGCATGTGTCAAATGTTATAGGGCGGCAAAGGGTGATGTTTTACCTCTGTTGTGTCCTGAAAAGCATGAGACCAATGTTATCAATCTAAGGTTTAAGTTAGAGATGGAAGTGGAATATAATGGAGTCGTAGCCAAGTTTTTTTTTTTTGGGATCGCTGGCGTCACAAATCCATTAGAATATCCAATTTTGCTGGATCAACTCGAACATAGAAATGTTTGTAAAGTTAAGTGGCAGCCAACTTGGGACAGTTGTTCTGTTCAAGGAGGATGA
Protein sequence

>Msa1306260

MVDTTKYKACVKCYRAAKGDVLPLLCPEKHETNVINLRFKLEMEVEYNGVVAKFFFFGIAGVTNPLEYPILLDQLEHRNVCKVKWQPTWDSCSVQGG*