AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1321680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1321680 MtrunA17_Chr8g0351501 100.000 106 0 0 1 106 1 106 2.35e-76 220
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1321680 AT5G18800.2 85.849 106 15 0 1 106 1 106 2.90e-65 192
Msa1321680 AT5G18800.1 85.849 106 15 0 1 106 1 106 2.90e-65 192
Msa1321680 AT3G06310.1 79.048 105 22 0 2 106 4 108 1.31e-60 181
Msa1321680 AT3G06310.3 79.048 105 22 0 2 106 4 108 1.31e-60 181
Msa1321680 AT3G06310.2 77.419 62 14 0 2 63 4 65 8.48e-32 107

Find 57 sgRNAs with CRISPR-Local

Find 283 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAAAAGATCAAATGAATT+TGG 0.156685 8_4:+27812909 None:intergenic
CCATCTCCTTTGTGCACTTT+TGG 0.157227 8_4:+27813097 None:intergenic
AAGAGTTATTGTGAGTATTA+AGG 0.202243 8_4:-27817270 Msa1321680:five_prime_UTR
AATAATATCACAGGGCAATT+TGG 0.235688 8_4:-27812954 Msa1321680:three_prime_UTR
TGACTACTTGCTACACATTC+AGG 0.240353 8_4:-27817160 Msa1321680:five_prime_UTR
AAGATGTCATTCGGAGAATT+TGG 0.243844 8_4:-27816660 Msa1321680:CDS
ACTAACAAAATACACAATTA+AGG 0.276169 8_4:+27817417 None:intergenic
AGAGTTATTGTGAGTATTAA+GGG 0.292469 8_4:-27817269 Msa1321680:five_prime_UTR
GAAGATTGATGGTGAAGATT+AGG 0.293815 8_4:+27816977 None:intergenic
GTTGGTAGTCTAAATGCTTC+TGG 0.304274 8_4:-27812853 Msa1321680:three_prime_UTR
AAGATTGATGGTGAAGATTA+GGG 0.339205 8_4:+27816978 None:intergenic
TAAGGGATTATTTAAGCGTT+AGG 0.343544 8_4:-27817252 Msa1321680:five_prime_UTR
CAAATGCTTCCTGCTCTTTG+CGG 0.352587 8_4:+27813025 None:intergenic
ATCAAATGAATTTGGGAAAC+AGG 0.360204 8_4:+27812917 None:intergenic
TGCTCTAAACTAAGACTGTT+TGG 0.367685 8_4:-27812530 Msa1321680:three_prime_UTR
CCCAACATCAGCGGTGTTGA+TGG 0.386535 8_4:-27816705 Msa1321680:CDS
ACAAAAGATCAAATGAATTT+GGG 0.389383 8_4:+27812910 None:intergenic
TCATGTGTTTGTTCACTTTG+TGG 0.408772 8_4:-27812575 Msa1321680:three_prime_UTR
TCCTGAAAAGTGTCTTGATA+AGG 0.409136 8_4:-27816606 Msa1321680:CDS
AGGTCATAATCGTTGTAGTT+TGG 0.415899 8_4:+27812681 None:intergenic
AAGGGATTATTTAAGCGTTA+GGG 0.427602 8_4:-27817251 Msa1321680:five_prime_UTR
GATGTTACTCGCTGTGTCCT+TGG 0.430847 8_4:-27816578 Msa1321680:CDS
ATAAGTCGAATTCATTTGTA+TGG 0.438882 8_4:+27813049 None:intergenic
ATAATAAGTAAGTCTATTGA+AGG 0.440218 8_4:+27817345 None:intergenic
GACTAACAAAGTGAACAATC+AGG 0.440774 8_4:-27817220 Msa1321680:five_prime_UTR
CCATCAACACCGCTGATGTT+GGG 0.462363 8_4:+27816705 None:intergenic
CATTGGGTTAAGATGTCATT+CGG 0.471473 8_4:-27816669 Msa1321680:CDS
GCCATCAACACCGCTGATGT+TGG 0.471695 8_4:+27816704 None:intergenic
GACTACTTGCTACACATTCA+GGG 0.481130 8_4:-27817159 Msa1321680:five_prime_UTR
ATTGTTGAAAATAATATCAC+AGG 0.482137 8_4:-27812963 Msa1321680:three_prime_UTR
CCTGAAAAGTGTCTTGATAA+GGG 0.482879 8_4:-27816605 Msa1321680:CDS
ACACCGCTGATGTTGGGATC+GGG 0.486192 8_4:+27816711 None:intergenic
CGACTTATGCCGCAAAGAGC+AGG 0.487600 8_4:-27813034 Msa1321680:CDS
TTAAATACAAAGTTATTTGG+TGG 0.496328 8_4:+27817103 None:intergenic
TGGCATCTTCGAAGCACATT+GGG 0.505873 8_4:-27816685 Msa1321680:CDS
TCGAGTGCTGTAGATGCTGC+TGG 0.511264 8_4:-27816737 Msa1321680:CDS
ATCTTTACCATTGACTATGT+TGG 0.515946 8_4:-27812871 Msa1321680:three_prime_UTR
GGTGTGGGAAGGAAGATTGA+TGG 0.533826 8_4:+27816966 None:intergenic
CCAAAAGTGCACAAAGGAGA+TGG 0.538195 8_4:-27813097 Msa1321680:CDS
AACACCGCTGATGTTGGGAT+CGG 0.539717 8_4:+27816710 None:intergenic
TCTGCGATAACATCTTGTGT+TGG 0.550933 8_4:-27812615 Msa1321680:three_prime_UTR
TAACGGGGCCGTAGTCACTT+TGG 0.552796 8_4:+27817135 None:intergenic
AAGAAGAAACTCACAGTCCA+AGG 0.560779 8_4:+27816561 None:intergenic
TAGACTACCAACATAGTCAA+TGG 0.563567 8_4:+27812864 None:intergenic
ATGGCATCTTCGAAGCACAT+TGG 0.565008 8_4:-27816686 Msa1321680:CDS
ATTGAATTGCAGTGTGGTGT+GGG 0.565127 8_4:+27816951 None:intergenic
TTCAGGGACCAAAGTGACTA+CGG 0.567206 8_4:-27817143 Msa1321680:five_prime_UTR
AATTGAATTGCAGTGTGGTG+TGG 0.596145 8_4:+27816950 None:intergenic
TGTGTTTGTTCACTTTGTGG+AGG 0.603174 8_4:-27812572 Msa1321680:three_prime_UTR
ACTAACAAAGTGAACAATCA+GGG 0.603688 8_4:-27817219 Msa1321680:five_prime_UTR
AATTGCAGTGTGGTGTGGGA+AGG 0.605305 8_4:+27816955 None:intergenic
TTGTTGAAAATAATATCACA+GGG 0.662122 8_4:-27812962 Msa1321680:three_prime_UTR
AAACCCGATCCCAACATCAG+CGG 0.696688 8_4:-27816714 Msa1321680:CDS
CACTTTGTGGAGGAACAATG+TGG 0.705871 8_4:-27812562 Msa1321680:three_prime_UTR
TCTACACCAAAAGTGCACAA+AGG 0.729329 8_4:-27813103 Msa1321680:CDS
AAGGAGATGGATGATTACGT+CGG 0.730179 8_4:-27813084 Msa1321680:CDS
ACTTGAATTGAATTGCAGTG+TGG 0.737957 8_4:+27816945 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAAATACAAAGTTATT+TGG + chr8_4:27812822-27812841 None:intergenic 10.0%
!!! CTGTTTTTATTTTAAAAATT+TGG - chr8_4:27814223-27814242 Msa1321680:intron 10.0%
!!! TTTTTTTTAATAGTTTTTCT+AGG + chr8_4:27814751-27814770 None:intergenic 10.0%
!!! TGATTTGTTTTTATTTAATA+TGG + chr8_4:27815438-27815457 None:intergenic 10.0%
!! CAGTAAAAAAAAAATTAATA+AGG + chr8_4:27815918-27815937 None:intergenic 10.0%
!!! CTTATTAATTTTTTTTTTAC+TGG - chr8_4:27815916-27815935 Msa1321680:intron 10.0%
!!! ATGTTTTTTTATAATTTATC+AGG - chr8_4:27816121-27816140 Msa1321680:intron 10.0%
!!! AACGAAATATTTTAATATCA+AGG + chr8_4:27812537-27812556 None:intergenic 15.0%
!!! ACAATAACTCTTTTTATATA+AGG + chr8_4:27812641-27812660 None:intergenic 15.0%
!! AATGAAATTGAAATTGAAAA+TGG + chr8_4:27813011-27813030 None:intergenic 15.0%
!! AAAGACATAACATAAATTAA+GGG + chr8_4:27813748-27813767 None:intergenic 15.0%
!! TTAAAATAAAAACAGAAGAT+CGG + chr8_4:27814218-27814237 None:intergenic 15.0%
!! AGCATTTGAAAATATTATAT+TGG - chr8_4:27814304-27814323 Msa1321680:intron 15.0%
!! CGATAATAATTACAAAATAT+TGG + chr8_4:27814992-27815011 None:intergenic 15.0%
!! AAATTTAATATATAGGAAGA+AGG + chr8_4:27815320-27815339 None:intergenic 15.0%
!! TAAATCAAGAAATATTTACA+AGG - chr8_4:27815560-27815579 Msa1321680:intron 15.0%
!!! TAATTTCTCCTTTTTTTTTA+TGG - chr8_4:27815966-27815985 Msa1321680:intron 15.0%
!!! TACAATTTAGTTTTCTATAT+AGG + chr8_4:27816400-27816419 None:intergenic 15.0%
!! ATAATAAGTAAGTCTATTGA+AGG + chr8_4:27812577-27812596 None:intergenic 20.0%
!!! TTAGAATTTTGATACATTGA+AGG - chr8_4:27812729-27812748 Msa1321680:three_prime_UTR 20.0%
!! TTAAATACAAAGTTATTTGG+TGG + chr8_4:27812819-27812838 None:intergenic 20.0%
!! AATTGAAAATTTGGGAAAAT+GGG + chr8_4:27813042-27813061 None:intergenic 20.0%
!! AAATTGAAAATTTGGGAAAA+TGG + chr8_4:27813043-27813062 None:intergenic 20.0%
!!! AAAATTGTTATGTGATTTTC+TGG + chr8_4:27813117-27813136 None:intergenic 20.0%
!!! AAATCACATAACAATTTTCT+CGG - chr8_4:27813119-27813138 Msa1321680:intron 20.0%
!!! TTTTGTTGATTTTGTTTATG+TGG - chr8_4:27813407-27813426 Msa1321680:intron 20.0%
!!! TTTCTCAAAACTAGTTTTAT+TGG - chr8_4:27813500-27813519 Msa1321680:intron 20.0%
!!! TTCTCAAAACTAGTTTTATT+GGG - chr8_4:27813501-27813520 Msa1321680:intron 20.0%
!!! TTGCATAAACTATTTTTCAT+AGG - chr8_4:27813696-27813715 Msa1321680:intron 20.0%
!! CAAAGACATAACATAAATTA+AGG + chr8_4:27813749-27813768 None:intergenic 20.0%
!! TTATGTTATGTCTTTGAATT+TGG - chr8_4:27813753-27813772 Msa1321680:intron 20.0%
!! TATGTTATGTCTTTGAATTT+GGG - chr8_4:27813754-27813773 Msa1321680:intron 20.0%
!!! TTTGATACCAATTTAGATTT+TGG - chr8_4:27813971-27813990 Msa1321680:intron 20.0%
!!! TTGATACCAATTTAGATTTT+GGG - chr8_4:27813972-27813991 Msa1321680:intron 20.0%
!! TGAAAAATGTGTTTATAAGT+AGG + chr8_4:27814049-27814068 None:intergenic 20.0%
!!! TACTTATAAACACATTTTTC+AGG - chr8_4:27814048-27814067 Msa1321680:intron 20.0%
!! CATTATGTTATCAATAAGTT+GGG - chr8_4:27814100-27814119 Msa1321680:intron 20.0%
!! ATTATGTTATCAATAAGTTG+GGG - chr8_4:27814101-27814120 Msa1321680:intron 20.0%
!! ATTTGTTTATTGATACAACA+AGG - chr8_4:27814408-27814427 Msa1321680:intron 20.0%
!! TGATATATGAATGAACATTA+TGG - chr8_4:27814802-27814821 Msa1321680:intron 20.0%
!!! TTATCGTCATTATATTTTCT+TGG - chr8_4:27815006-27815025 Msa1321680:intron 20.0%
!! GTTATTGCATTATAAACAAT+TGG - chr8_4:27815213-27815232 Msa1321680:intron 20.0%
!! AGGACTCAAATTTAATATAT+AGG + chr8_4:27815327-27815346 None:intergenic 20.0%
!! TTGCATATTTAATAATCTTG+TGG - chr8_4:27815687-27815706 Msa1321680:intron 20.0%
!!! TTAAAAGTACCATATTTTGA+TGG - chr8_4:27815824-27815843 Msa1321680:intron 20.0%
!! TTCTATCAAAAACCATATTA+CGG + chr8_4:27816173-27816192 None:intergenic 20.0%
!! ACTAGTTAATAAAAGCAATT+AGG + chr8_4:27816463-27816482 None:intergenic 20.0%
!!! ATTCATTTTGATTTGGTTTT+TGG + chr8_4:27816510-27816529 None:intergenic 20.0%
!! ACCAAATCAAAATGAATAAT+AGG - chr8_4:27816513-27816532 Msa1321680:intron 20.0%
!!! TCCTATTATTCATTTTGATT+TGG + chr8_4:27816517-27816536 None:intergenic 20.0%
!! ATTTGAGAAAAAATGTTCTT+TGG - chr8_4:27816912-27816931 Msa1321680:intron 20.0%
!! ATTGTTGAAAATAATATCAC+AGG - chr8_4:27816956-27816975 Msa1321680:five_prime_UTR 20.0%
!! TTGTTGAAAATAATATCACA+GGG - chr8_4:27816957-27816976 Msa1321680:five_prime_UTR 20.0%
!! ACAAAAGATCAAATGAATTT+GGG + chr8_4:27817012-27817031 None:intergenic 20.0%
! AAGAGTTATTGTGAGTATTA+AGG - chr8_4:27812649-27812668 Msa1321680:three_prime_UTR 25.0%
! AGAGTTATTGTGAGTATTAA+GGG - chr8_4:27812650-27812669 Msa1321680:three_prime_UTR 25.0%
!!! TTTGGTGGTTTTTTTTGTAA+CGG + chr8_4:27812804-27812823 None:intergenic 25.0%
! TTGAAATTGAAAATGGAGAA+TGG + chr8_4:27813004-27813023 None:intergenic 25.0%
!! TGATTTTCTTGAGAAAATGA+AGG + chr8_4:27813093-27813112 None:intergenic 25.0%
! TTGATTTCAATGTGTTTGTT+TGG - chr8_4:27813441-27813460 Msa1321680:intron 25.0%
! TGTTTGGTTGAATAAAATAG+AGG - chr8_4:27813457-27813476 Msa1321680:intron 25.0%
! AAAAAATAAGCCAATCCAAA+CGG + chr8_4:27813528-27813547 None:intergenic 25.0%
! TTTATAAGTTTGTCATGTGA+AGG - chr8_4:27813595-27813614 Msa1321680:intron 25.0%
! CAAAAATAAGTCAATCCAAA+CGG - chr8_4:27813721-27813740 Msa1321680:intron 25.0%
! AAAAATAAGTCAATCCAAAC+GGG - chr8_4:27813722-27813741 Msa1321680:intron 25.0%
! TTGAAAATGTGTTCATTAGT+AGG + chr8_4:27813829-27813848 None:intergenic 25.0%
! ATCTAAATTGGTATCAAACT+CGG + chr8_4:27813969-27813988 None:intergenic 25.0%
! CAAACTTAAACCTAGAAAAT+CGG - chr8_4:27814001-27814020 Msa1321680:intron 25.0%
! AAACTTAAACCTAGAAAATC+GGG - chr8_4:27814002-27814021 Msa1321680:intron 25.0%
! GCATTATGTTATCAATAAGT+TGG - chr8_4:27814099-27814118 Msa1321680:intron 25.0%
! TTGAAATGAATGTTTGTGTT+TGG - chr8_4:27814254-27814273 Msa1321680:intron 25.0%
! TGAAATGAATGTTTGTGTTT+GGG - chr8_4:27814255-27814274 Msa1321680:intron 25.0%
!!! TTTTGAGTACTAATACGTAA+TGG - chr8_4:27814523-27814542 Msa1321680:intron 25.0%
!! ATATCTTAAACTGATTGGTT+CGG - chr8_4:27814547-27814566 Msa1321680:intron 25.0%
! ACTTAATAATTCCTAGTTCA+TGG - chr8_4:27814615-27814634 Msa1321680:intron 25.0%
! TATGTTAGAATTAACATCGA+GGG + chr8_4:27814918-27814937 None:intergenic 25.0%
! ATATGTTAGAATTAACATCG+AGG + chr8_4:27814919-27814938 None:intergenic 25.0%
! TAGAAGGAAACAAGAAAAAA+GGG + chr8_4:27815138-27815157 None:intergenic 25.0%
! TTAGAAGGAAACAAGAAAAA+AGG + chr8_4:27815139-27815158 None:intergenic 25.0%
!!! ATATCCAAGACTATTTTTCT+AGG + chr8_4:27815272-27815291 None:intergenic 25.0%
! TTTCTCAATATTCAAAACTG+AGG + chr8_4:27815347-27815366 None:intergenic 25.0%
! GATAGAATAACGACTAAAAA+GGG + chr8_4:27815470-27815489 None:intergenic 25.0%
! TGATAGAATAACGACTAAAA+AGG + chr8_4:27815471-27815490 None:intergenic 25.0%
!! TTGATTTACTGTTGATTGAT+TGG + chr8_4:27815548-27815567 None:intergenic 25.0%
! AAATAGGATCCATCAAAATA+TGG + chr8_4:27815836-27815855 None:intergenic 25.0%
! CTTTCAATATCTGAAAAACT+AGG + chr8_4:27815886-27815905 None:intergenic 25.0%
!!! TCTCCTTTTTTTTTATGGTA+AGG - chr8_4:27815971-27815990 Msa1321680:intron 25.0%
! CTTCCTTACCATAAAAAAAA+AGG + chr8_4:27815977-27815996 None:intergenic 25.0%
! ATAATTTATCAGGAAAGACT+TGG - chr8_4:27816131-27816150 Msa1321680:intron 25.0%
!! TGGTTTTTGATAGAATGTTA+TGG - chr8_4:27816178-27816197 Msa1321680:intron 25.0%
!! TGCTTTTATTAACTAGTAGT+AGG - chr8_4:27816466-27816485 Msa1321680:intron 25.0%
!!! TAGTTTAGTTGTTTTTGATG+CGG - chr8_4:27816641-27816660 Msa1321680:CDS 25.0%
! ATATTATTTCGACATTCTTG+AGG + chr8_4:27816764-27816783 None:intergenic 25.0%
! ATAAGTCGAATTCATTTGTA+TGG + chr8_4:27816873-27816892 None:intergenic 25.0%
! GACAAAAGATCAAATGAATT+TGG + chr8_4:27817013-27817032 None:intergenic 25.0%
TAAGGGATTATTTAAGCGTT+AGG - chr8_4:27812667-27812686 Msa1321680:three_prime_UTR 30.0%
AAGGGATTATTTAAGCGTTA+GGG - chr8_4:27812668-27812687 Msa1321680:three_prime_UTR 30.0%
ACTAACAAAGTGAACAATCA+GGG - chr8_4:27812700-27812719 Msa1321680:three_prime_UTR 30.0%
!!! TTGGTGGTTTTTTTTGTAAC+GGG + chr8_4:27812803-27812822 None:intergenic 30.0%
!! AAGATTGATGGTGAAGATTA+GGG + chr8_4:27812944-27812963 None:intergenic 30.0%
AGGGGGAAAATTGAAAATTT+GGG + chr8_4:27813050-27813069 None:intergenic 30.0%
AAGGGGGAAAATTGAAAATT+TGG + chr8_4:27813051-27813070 None:intergenic 30.0%
TGAAGGAAAATGGAATGAAA+GGG + chr8_4:27813076-27813095 None:intergenic 30.0%
ATGAAGGAAAATGGAATGAA+AGG + chr8_4:27813077-27813096 None:intergenic 30.0%
CTTGAGAAAATGAAGGAAAA+TGG + chr8_4:27813086-27813105 None:intergenic 30.0%
!! TACCAATTTAGATTTTGGGT+TGG - chr8_4:27813976-27813995 Msa1321680:intron 30.0%
!! CGATTTTCTAGGTTTAAGTT+TGG + chr8_4:27814003-27814022 None:intergenic 30.0%
TCTTATCTAATGTAAGACTC+TGG - chr8_4:27814076-27814095 Msa1321680:intron 30.0%
CTTATCTAATGTAAGACTCT+GGG - chr8_4:27814077-27814096 Msa1321680:intron 30.0%
!! TGCAATTGTTTCTAATTGCT+TGG - chr8_4:27814371-27814390 Msa1321680:intron 30.0%
TAGATACTAAGACCTTGATT+AGG - chr8_4:27814431-27814450 Msa1321680:intron 30.0%
AGATACTAAGACCTTGATTA+GGG - chr8_4:27814432-27814451 Msa1321680:intron 30.0%
ATGGCATATCTTAAACTGAT+TGG - chr8_4:27814542-27814561 Msa1321680:intron 30.0%
TAATAATTCCTAGTTCATGG+TGG - chr8_4:27814618-27814637 Msa1321680:intron 30.0%
GACCTATAGATTCTGTAATA+AGG - chr8_4:27814677-27814696 Msa1321680:intron 30.0%
CTCCTTATTACAGAATCTAT+AGG + chr8_4:27814682-27814701 None:intergenic 30.0%
AATAAGGAGAGTAGATCAAA+TGG - chr8_4:27814693-27814712 Msa1321680:intron 30.0%
AAATCTAGAGAGTAATGAGT+TGG - chr8_4:27814768-27814787 Msa1321680:intron 30.0%
TTCTAACATATCTGCTTCAA+AGG - chr8_4:27814928-27814947 Msa1321680:intron 30.0%
TCTAACATATCTGCTTCAAA+GGG - chr8_4:27814929-27814948 Msa1321680:intron 30.0%
! TCGTCATTATATTTTCTTGG+AGG - chr8_4:27815009-27815028 Msa1321680:intron 30.0%
TTATCAAGCAGACAATTAGA+AGG + chr8_4:27815154-27815173 None:intergenic 30.0%
CTAATTGTCTGCTTGATAAA+TGG - chr8_4:27815155-27815174 Msa1321680:intron 30.0%
!! AAGACTATTTTTCTAGGTTC+AGG + chr8_4:27815266-27815285 None:intergenic 30.0%
TGAACCTAGAAAAATAGTCT+TGG - chr8_4:27815265-27815284 Msa1321680:intron 30.0%
! GAAAAATAGTCTTGGATATC+TGG - chr8_4:27815273-27815292 Msa1321680:intron 30.0%
TGTGTGATAAGAGTAAAACA+TGG + chr8_4:27815370-27815389 None:intergenic 30.0%
ATTGAAGTATATCTCTGCAT+GGG - chr8_4:27815627-27815646 Msa1321680:intron 30.0%
!! TTCTAAAAACAAAGTACCGA+CGG + chr8_4:27815788-27815807 None:intergenic 30.0%
TATCTGAAAAACTAGGAGTA+TGG + chr8_4:27815879-27815898 None:intergenic 30.0%
GAAATTAAGCTTTCCTCAAA+GGG + chr8_4:27815953-27815972 None:intergenic 30.0%
AGAAATTAAGCTTTCCTCAA+AGG + chr8_4:27815954-27815973 None:intergenic 30.0%
!!! ACGATTGAGTTTTAATTCAG+AGG - chr8_4:27816279-27816298 Msa1321680:intron 30.0%
CTATATAGGTATTTCCCATT+CGG + chr8_4:27816386-27816405 None:intergenic 30.0%
AAAAGCAATTAGGTACAGTT+CGG + chr8_4:27816453-27816472 None:intergenic 30.0%
TTATTAACTAGTAGTAGGAC+AGG - chr8_4:27816471-27816490 Msa1321680:intron 30.0%
CTAACAAGTATGTTCAATCT+GGG + chr8_4:27816604-27816623 None:intergenic 30.0%
ACTAACAAGTATGTTCAATC+TGG + chr8_4:27816605-27816624 None:intergenic 30.0%
!!! AGTTTAGTTGTTTTTGATGC+GGG - chr8_4:27816642-27816661 Msa1321680:CDS 30.0%
TTCAGACAAACAATGATAGA+AGG + chr8_4:27816713-27816732 None:intergenic 30.0%
AATAATATCACAGGGCAATT+TGG - chr8_4:27816965-27816984 Msa1321680:five_prime_UTR 30.0%
ATCAAATGAATTTGGGAAAC+AGG + chr8_4:27817005-27817024 None:intergenic 30.0%
! ATCTTTACCATTGACTATGT+TGG - chr8_4:27817048-27817067 Msa1321680:five_prime_UTR 30.0%
!! CTTATTTTCAAACATGCACT+CGG + chr8_4:27817281-27817300 None:intergenic 30.0%
GACTAACAAAGTGAACAATC+AGG - chr8_4:27812699-27812718 Msa1321680:three_prime_UTR 35.0%
!!! TGGTGGTTTTTTTTGTAACG+GGG + chr8_4:27812802-27812821 None:intergenic 35.0%
!! GAAGATTGATGGTGAAGATT+AGG + chr8_4:27812945-27812964 None:intergenic 35.0%
ACTTGAATTGAATTGCAGTG+TGG + chr8_4:27812977-27812996 None:intergenic 35.0%
AAATGGAATGAAAGGGGAAA+GGG + chr8_4:27813069-27813088 None:intergenic 35.0%
AAAATGGAATGAAAGGGGAA+AGG + chr8_4:27813070-27813089 None:intergenic 35.0%
GAAGGAAAATGGAATGAAAG+GGG + chr8_4:27813075-27813094 None:intergenic 35.0%
!!! TTTCGTGTTTTTTCAGGTGA+AGG - chr8_4:27813144-27813163 Msa1321680:intron 35.0%
CATTGGGTTAAGATGTCATT+CGG - chr8_4:27813250-27813269 Msa1321680:intron 35.0%
AAGATGTCATTCGGAGAATT+TGG - chr8_4:27813259-27813278 Msa1321680:intron 35.0%
!! TCCTGAAAAGTGTCTTGATA+AGG - chr8_4:27813313-27813332 Msa1321680:intron 35.0%
!! CCTGAAAAGTGTCTTGATAA+GGG - chr8_4:27813314-27813333 Msa1321680:intron 35.0%
AAAATAGAGGTGACTAGAGT+AGG - chr8_4:27813470-27813489 Msa1321680:intron 35.0%
CTGACTTAACCCTATAAAAC+CGG - chr8_4:27813783-27813802 Msa1321680:intron 35.0%
ATACTCTAGATGACCAATTC+TGG + chr8_4:27813895-27813914 None:intergenic 35.0%
! CATCTAGAGTATAACATGAG+TGG - chr8_4:27813903-27813922 Msa1321680:intron 35.0%
GTCCAACCCAAAATCTAAAT+TGG + chr8_4:27813981-27814000 None:intergenic 35.0%
! TGTTATCAATAAGTTGGGGT+AGG - chr8_4:27814105-27814124 Msa1321680:intron 35.0%
!! AATTTTGACACCATTCAGCT+AGG + chr8_4:27814350-27814369 None:intergenic 35.0%
!! CAAAGGGTACTTAGTACATA+TGG - chr8_4:27814945-27814964 Msa1321680:intron 35.0%
AGTACATATGGAAGCAAAAG+TGG - chr8_4:27814957-27814976 Msa1321680:intron 35.0%
TCTAGGTTCAGGTATAAACA+CGG + chr8_4:27815255-27815274 None:intergenic 35.0%
CATTGAAGTATATCTCTGCA+TGG - chr8_4:27815626-27815645 Msa1321680:intron 35.0%
GTTGAAGTCAGCAATAAACA+GGG + chr8_4:27815722-27815741 None:intergenic 35.0%
CTTATTAGGATCCATGCATT+TGG + chr8_4:27815754-27815773 None:intergenic 35.0%
CGGTTTAGTGATGACTTATT+AGG + chr8_4:27815768-27815787 None:intergenic 35.0%
ATAAGTCATCACTAAACCGT+CGG - chr8_4:27815769-27815788 Msa1321680:intron 35.0%
! GATGGATCCTATTTTATCAC+TGG - chr8_4:27815842-27815861 Msa1321680:intron 35.0%
! AAAAACTAGGAGTATGGTAG+TGG + chr8_4:27815873-27815892 None:intergenic 35.0%
ACTGTTAGATACATTAGCTC+TGG - chr8_4:27816011-27816030 Msa1321680:intron 35.0%
! TAATGAGTTGGACCGTAATA+TGG - chr8_4:27816158-27816177 Msa1321680:intron 35.0%
! TTAACCCATTTAAAGTGGGT+TGG + chr8_4:27816223-27816242 None:intergenic 35.0%
!! AACCCACTTTAAATGGGTTA+AGG - chr8_4:27816222-27816241 Msa1321680:intron 35.0%
! AGCCTTAACCCATTTAAAGT+GGG + chr8_4:27816227-27816246 None:intergenic 35.0%
AAGCCTTAACCCATTTAAAG+TGG + chr8_4:27816228-27816247 None:intergenic 35.0%
!! ACTTTAAATGGGTTAAGGCT+TGG - chr8_4:27816227-27816246 Msa1321680:intron 35.0%
AGGAAAACTAAACCAAGGTT+CGG - chr8_4:27816337-27816356 Msa1321680:intron 35.0%
CGGTTCATAAAGAATCAACT+CGG + chr8_4:27816433-27816452 None:intergenic 35.0%
TTAACAGTCTGACTCTGATA+CGG + chr8_4:27816560-27816579 None:intergenic 35.0%
!!! ATGCTTGTTTTGTAGTCTCA+AGG - chr8_4:27816792-27816811 Msa1321680:intron 35.0%
TAGACTACCAACATAGTCAA+TGG + chr8_4:27817058-27817077 None:intergenic 35.0%
!!! CTGAGAAGAAGTTTTGAAGT+TGG - chr8_4:27817136-27817155 Msa1321680:five_prime_UTR 35.0%
AGGTCATAATCGTTGTAGTT+TGG + chr8_4:27817241-27817260 None:intergenic 35.0%
CGGTTAGCAAAAAACTAACA+AGG + chr8_4:27817261-27817280 None:intergenic 35.0%
TCATGTGTTTGTTCACTTTG+TGG - chr8_4:27817344-27817363 Msa1321680:five_prime_UTR 35.0%
! TGCTCTAAACTAAGACTGTT+TGG - chr8_4:27817389-27817408 Msa1321680:five_prime_UTR 35.0%
! TGACTACTTGCTACACATTC+AGG - chr8_4:27812759-27812778 Msa1321680:three_prime_UTR 40.0%
! GACTACTTGCTACACATTCA+GGG - chr8_4:27812760-27812779 Msa1321680:three_prime_UTR 40.0%
ATTGAATTGCAGTGTGGTGT+GGG + chr8_4:27812971-27812990 None:intergenic 40.0%
AATTGAATTGCAGTGTGGTG+TGG + chr8_4:27812972-27812991 None:intergenic 40.0%
AATGGAATGAAAGGGGAAAG+GGG + chr8_4:27813068-27813087 None:intergenic 40.0%
!!! TCGGTGTTTCGTGTTTTTTC+AGG - chr8_4:27813138-27813157 Msa1321680:intron 40.0%
! CCCTTATCAAGACACTTTTC+AGG + chr8_4:27813317-27813336 None:intergenic 40.0%
AAGAAGAAACTCACAGTCCA+AGG + chr8_4:27813361-27813380 None:intergenic 40.0%
!!! CTAGTTTTATTGGGTCCGTT+TGG - chr8_4:27813510-27813529 Msa1321680:intron 40.0%
TTTATTGGGTCCGTTTGGAT+TGG - chr8_4:27813515-27813534 Msa1321680:intron 40.0%
ACATAAATTAAGGGCCCGTT+TGG + chr8_4:27813739-27813758 None:intergenic 40.0%
!! CCTTACAAGCCGGTTTTATA+GGG + chr8_4:27813795-27813814 None:intergenic 40.0%
!! ACCTTACAAGCCGGTTTTAT+AGG + chr8_4:27813796-27813815 None:intergenic 40.0%
!! TATAAAACCGGCTTGTAAGG+TGG - chr8_4:27813795-27813814 Msa1321680:intron 40.0%
ATATTTGCCACCTTACAAGC+CGG + chr8_4:27813805-27813824 None:intergenic 40.0%
GAGTCTCATTGGATGAGATA+TGG + chr8_4:27813852-27813871 None:intergenic 40.0%
GGAGTCTATCCAAGATCTAT+CGG + chr8_4:27813948-27813967 None:intergenic 40.0%
TCGGCTGACAAATACAGAAA+TGG + chr8_4:27814199-27814218 None:intergenic 40.0%
TTGAATGCCTAACTCAGCTA+AGG + chr8_4:27814282-27814301 None:intergenic 40.0%
CAGCTAGGTTCAAGAGAAAA+AGG + chr8_4:27814335-27814354 None:intergenic 40.0%
TTCTCTTGAACCTAGCTGAA+TGG - chr8_4:27814337-27814356 Msa1321680:intron 40.0%
!! TTTTCGAGCATCCCTAATCA+AGG + chr8_4:27814446-27814465 None:intergenic 40.0%
ACAGCATGTTCTATTGTTGC+TGG - chr8_4:27814480-27814499 Msa1321680:intron 40.0%
AAACTCGGCTTAGATGGTTT+GGG - chr8_4:27814641-27814660 Msa1321680:intron 40.0%
! TTAGATGGTTTGGGCATGTA+GGG - chr8_4:27814650-27814669 Msa1321680:intron 40.0%
AAGGAGAGTAGATCAAATGG+AGG - chr8_4:27814696-27814715 Msa1321680:intron 40.0%
AGGAGAGTAGATCAAATGGA+GGG - chr8_4:27814697-27814716 Msa1321680:intron 40.0%
AGTCAAATCACGAGAGAAAG+AGG - chr8_4:27814721-27814740 Msa1321680:intron 40.0%
!! TAGAGAGTAATGAGTTGGTG+AGG - chr8_4:27814773-27814792 Msa1321680:intron 40.0%
!! AGAGAGTAATGAGTTGGTGA+GGG - chr8_4:27814774-27814793 Msa1321680:intron 40.0%
TCAGCAATAAACAGGGATGA+CGG + chr8_4:27815715-27815734 None:intergenic 40.0%
CGTTGAAGTCAGCAATAAAC+AGG + chr8_4:27815723-27815742 None:intergenic 40.0%
ACGTCTTATCACCAAATGCA+TGG - chr8_4:27815740-27815759 Msa1321680:intron 40.0%
GGTGTGACCAGTGATAAAAT+AGG + chr8_4:27815852-27815871 None:intergenic 40.0%
AGACTTGGAGACTAATGAGT+TGG - chr8_4:27816146-27816165 Msa1321680:intron 40.0%
TGTAGCCAACCCACTTTAAA+TGG - chr8_4:27816215-27816234 Msa1321680:intron 40.0%
GTAGCCAACCCACTTTAAAT+GGG - chr8_4:27816216-27816235 Msa1321680:intron 40.0%
AATTCAGAGGACTGCTTAGT+AGG - chr8_4:27816292-27816311 Msa1321680:intron 40.0%
CAGGAAGGAAAACTAAACCA+AGG - chr8_4:27816332-27816351 Msa1321680:intron 40.0%
! TTCTGTGTTTCTGAACCGAA+TGG - chr8_4:27816368-27816387 Msa1321680:intron 40.0%
! TCTGTGTTTCTGAACCGAAT+GGG - chr8_4:27816369-27816388 Msa1321680:intron 40.0%
! TCTGACTCTGATACGGTTTT+TGG + chr8_4:27816553-27816572 None:intergenic 40.0%
GGAACTATCTGTCACAGTTT+GGG + chr8_4:27816583-27816602 None:intergenic 40.0%
! TCTACACCAAAAGTGCACAA+AGG - chr8_4:27816816-27816835 Msa1321680:intron 40.0%
AAGGAGATGGATGATTACGT+CGG - chr8_4:27816835-27816854 Msa1321680:intron 40.0%
!! GTTGGTAGTCTAAATGCTTC+TGG - chr8_4:27817066-27817085 Msa1321680:five_prime_UTR 40.0%
TCTGCGATAACATCTTGTGT+TGG - chr8_4:27817304-27817323 Msa1321680:five_prime_UTR 40.0%
TGTGTTTGTTCACTTTGTGG+AGG - chr8_4:27817347-27817366 Msa1321680:five_prime_UTR 40.0%
TTCAGGGACCAAAGTGACTA+CGG - chr8_4:27812776-27812795 Msa1321680:three_prime_UTR 45.0%
ATGGAATGAAAGGGGAAAGG+GGG + chr8_4:27813067-27813086 None:intergenic 45.0%
ATGGCATCTTCGAAGCACAT+TGG - chr8_4:27813233-27813252 Msa1321680:intron 45.0%
TGGCATCTTCGAAGCACATT+GGG - chr8_4:27813234-27813253 Msa1321680:intron 45.0%
!! CCCTATAAAACCGGCTTGTA+AGG - chr8_4:27813792-27813811 Msa1321680:intron 45.0%
!! GTGAGTGTTGAGAGTCTCAT+TGG + chr8_4:27813863-27813882 None:intergenic 45.0%
ATGAGTGGCTCAGTAGTAGT+AGG - chr8_4:27813918-27813937 Msa1321680:intron 45.0%
GTAGGTGATCCGATAGATCT+TGG - chr8_4:27813936-27813955 Msa1321680:intron 45.0%
! CCTTACAAGCCCGATTTTCT+AGG + chr8_4:27814014-27814033 None:intergenic 45.0%
CCTAGAAAATCGGGCTTGTA+AGG - chr8_4:27814011-27814030 Msa1321680:intron 45.0%
! TTTGGGACCTTAGCTGAGTT+AGG - chr8_4:27814272-27814291 Msa1321680:intron 45.0%
! CCTAGTTCATGGTGGAAACT+CGG - chr8_4:27814626-27814645 Msa1321680:intron 45.0%
GAAACTCGGCTTAGATGGTT+TGG - chr8_4:27814640-27814659 Msa1321680:intron 45.0%
! CTTAGATGGTTTGGGCATGT+AGG - chr8_4:27814649-27814668 Msa1321680:intron 45.0%
TAAGCTTTCCTCAAAGGGCA+AGG + chr8_4:27815948-27815967 None:intergenic 45.0%
ACTGCTTAGTAGGCTGTTCA+TGG - chr8_4:27816302-27816321 Msa1321680:intron 45.0%
GGGAACTATCTGTCACAGTT+TGG + chr8_4:27816584-27816603 None:intergenic 45.0%
GGCGATACATACTTGTCAGT+AGG + chr8_4:27816692-27816711 None:intergenic 45.0%
! CCATCTCCTTTGTGCACTTT+TGG + chr8_4:27816825-27816844 None:intergenic 45.0%
! CCAAAAGTGCACAAAGGAGA+TGG - chr8_4:27816822-27816841 Msa1321680:intron 45.0%
CAAATGCTTCCTGCTCTTTG+CGG + chr8_4:27816897-27816916 None:intergenic 45.0%
CACTTTGTGGAGGAACAATG+TGG - chr8_4:27817357-27817376 Msa1321680:five_prime_UTR 45.0%
GGTGTGGGAAGGAAGATTGA+TGG + chr8_4:27812956-27812975 None:intergenic 50.0%
AATTGCAGTGTGGTGTGGGA+AGG + chr8_4:27812967-27812986 None:intergenic 50.0%
AAACCCGATCCCAACATCAG+CGG - chr8_4:27813205-27813224 Msa1321680:intron 50.0%
AACACCGCTGATGTTGGGAT+CGG + chr8_4:27813212-27813231 None:intergenic 50.0%
CCATCAACACCGCTGATGTT+GGG + chr8_4:27813217-27813236 None:intergenic 50.0%
GATGTTACTCGCTGTGTCCT+TGG - chr8_4:27813341-27813360 Msa1321680:intron 50.0%
CTCTCAACACTCACTCCTCA+CGG - chr8_4:27813869-27813888 Msa1321680:intron 50.0%
CCGAGTTTCCACCATGAACT+AGG + chr8_4:27814629-27814648 None:intergenic 50.0%
TGGTGGAAACTCGGCTTAGA+TGG - chr8_4:27814635-27814654 Msa1321680:intron 50.0%
GGATGATTCCTTGCCCTTTG+AGG - chr8_4:27815937-27815956 Msa1321680:intron 50.0%
TAGTAGGCTGTTCATGGCTC+AGG - chr8_4:27816308-27816327 Msa1321680:intron 50.0%
!! TAAACCAAGGTTCGGTGCAC+TGG - chr8_4:27816345-27816364 Msa1321680:intron 50.0%
TAACGGGGCCGTAGTCACTT+TGG + chr8_4:27812787-27812806 None:intergenic 55.0%
! TCGAGTGCTGTAGATGCTGC+TGG - chr8_4:27813182-27813201 Msa1321680:intron 55.0%
ACACCGCTGATGTTGGGATC+GGG + chr8_4:27813211-27813230 None:intergenic 55.0%
! CCCAACATCAGCGGTGTTGA+TGG - chr8_4:27813214-27813233 Msa1321680:intron 55.0%
GCCATCAACACCGCTGATGT+TGG + chr8_4:27813218-27813237 None:intergenic 55.0%
TCACTCCTCACGGCCAGAAT+TGG - chr8_4:27813879-27813898 Msa1321680:intron 55.0%
GATGACCAATTCTGGCCGTG+AGG + chr8_4:27813887-27813906 None:intergenic 55.0%
GTTCATGGCTCAGGTCAGGA+AGG - chr8_4:27816317-27816336 Msa1321680:intron 55.0%
AGAACCAGTGCACCGAACCT+TGG + chr8_4:27816352-27816371 None:intergenic 55.0%
CGACTTATGCCGCAAAGAGC+AGG - chr8_4:27816885-27816904 Msa1321680:intron 55.0%
GGCTGTTCATGGCTCAGGTC+AGG - chr8_4:27816313-27816332 Msa1321680:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 27812504 27817437 27812504 ID=Msa1321680;Name=Msa1321680
chr8_4 mRNA 27812504 27817437 27812504 ID=Msa1321680-mRNA-1;Parent=Msa1321680;Name=Msa1321680-mRNA-1;_AED=0.07;_eAED=0.07;_QI=510|1|1|1|0.5|0.33|3|498|106
chr8_4 exon 27812504 27813134 27812504 ID=Msa1321680-mRNA-1:exon:9375;Parent=Msa1321680-mRNA-1
chr8_4 exon 27816575 27816781 27816575 ID=Msa1321680-mRNA-1:exon:9374;Parent=Msa1321680-mRNA-1
chr8_4 exon 27816947 27817437 27816947 ID=Msa1321680-mRNA-1:exon:9373;Parent=Msa1321680-mRNA-1
chr8_4 five_prime_UTR 27816947 27817437 27816947 ID=Msa1321680-mRNA-1:five_prime_utr;Parent=Msa1321680-mRNA-1
chr8_4 five_prime_UTR 27816763 27816781 27816763 ID=Msa1321680-mRNA-1:five_prime_utr;Parent=Msa1321680-mRNA-1
chr8_4 CDS 27816575 27816762 27816575 ID=Msa1321680-mRNA-1:cds;Parent=Msa1321680-mRNA-1
chr8_4 CDS 27813002 27813134 27813002 ID=Msa1321680-mRNA-1:cds;Parent=Msa1321680-mRNA-1
chr8_4 three_prime_UTR 27812504 27813001 27812504 ID=Msa1321680-mRNA-1:three_prime_utr;Parent=Msa1321680-mRNA-1
Gene Sequence

>Msa1321680

ATGTCGAGTGCTGTAGATGCTGCTGGAAACCCGATCCCAACATCAGCGGTGTTGATGGCATCTTCGAAGCACATTGGGTTAAGATGTCATTCGGAGAATTTGGAGTTTCTGAAATGTAAGAAGAAAGATCAGAATCCTGAAAAGTGTCTTGATAAGGGACGTGATGTTACTCGCTGTGTCCTTGGACTTCTCAAGGATCTACACCAAAAGTGCACAAAGGAGATGGATGATTACGTCGGTTGCATGTACTACCATACAAATGAATTCGACTTATGCCGCAAAGAGCAGGAAGCATTTGAGAAAAAATGTTCTTTGGAATGA
Protein sequence

>Msa1321680

MSSAVDAAGNPIPTSAVLMASSKHIGLRCHSENLEFLKCKKKDQNPEKCLDKGRDVTRCVLGLLKDLHQKCTKEMDDYVGCMYYHTNEFDLCRKEQEAFEKKCSLE*