AlfalfaGEDB | Msa1321680

M. sativa cultivar zhongmu-4 / Msa1321680

KEGG: K03952
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005746 GO:0005747 GO:0009536 GO:0016020 GO:0019866 GO:0030964 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045271 GO:0070469 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:1902494 GO:1990204
PFAM: PF16860.8

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1321680	sp\|Q8LGE7\|NDA8B_ARATH	85.849	106	15	0	1	106	1	106	2.86e-64	192

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1321680	B7FN64	100.000	106	0	0	1	106	1	106	1.22e-72	220

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0052610	Msa1321680	0.808484	3.027174e-50	-8.615850e-47
Msa0103240	Msa1321680	0.806216	9.160661e-50	-8.615850e-47
Msa0167480	Msa1321680	0.806000	1.017467e-49	-8.615850e-47
Msa0171850	Msa1321680	0.808683	2.745005e-50	-8.615850e-47
Msa0226460	Msa1321680	0.800517	1.388844e-48	-8.615850e-47
Msa0313880	Msa1321680	0.801280	9.698720e-49	-8.615850e-47
Msa0738100	Msa1321680	0.808816	2.572028e-50	-8.615850e-47
Msa0750920	Msa1321680	0.802772	4.788209e-49	-8.615850e-47
Msa1079230	Msa1321680	0.801622	8.253317e-49	-8.615850e-47
Msa1143650	Msa1321680	0.804748	1.861596e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1321680	MtrunA17_Chr8g0351501	100.000	106	0	0	1	106	1	106	2.35e-76	220

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1321680	AT5G18800.2	85.849	106	15	1	106	1	106	2.90e-65	192
Msa1321680	AT5G18800.1	85.849	106	15	1	106	1	106	2.90e-65	192
Msa1321680	AT3G06310.1	79.048	105	22	2	106	4	108	1.31e-60	181
Msa1321680	AT3G06310.3	79.048	105	22	2	106	4	108	1.31e-60	181
Msa1321680	AT3G06310.2	77.419	62	14	2	63	4	65	8.48e-32	107

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Find 57 sgRNAs with CRISPR-Local

Find 283 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCCGATCCCAACATCAG+CGG	0.696688	8_4:-27816714	Msa1321680:CDS
AACACCGCTGATGTTGGGAT+CGG	0.539717	8_4:+27816710	None:intergenic
AAGAAGAAACTCACAGTCCA+AGG	0.560779	8_4:+27816561	None:intergenic
AAGAGTTATTGTGAGTATTA+AGG	0.202243	8_4:-27817270	Msa1321680:five_prime_UTR
AAGATGTCATTCGGAGAATT+TGG	0.243844	8_4:-27816660	Msa1321680:CDS
AAGATTGATGGTGAAGATTA+GGG	0.339205	8_4:+27816978	None:intergenic
AAGGAGATGGATGATTACGT+CGG	0.730179	8_4:-27813084	Msa1321680:CDS
AAGGGATTATTTAAGCGTTA+GGG	0.427602	8_4:-27817251	Msa1321680:five_prime_UTR
AATAATATCACAGGGCAATT+TGG	0.235688	8_4:-27812954	Msa1321680:three_prime_UTR
AATTGAATTGCAGTGTGGTG+TGG	0.596145	8_4:+27816950	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAGGGATTATTTAAGCGTT+AGG	-	chr8_4:27812667-27812686	Msa1321680:three_prime_UTR	30.0%
AAGGGATTATTTAAGCGTTA+GGG	-	chr8_4:27812668-27812687	Msa1321680:three_prime_UTR	30.0%
ACTAACAAAGTGAACAATCA+GGG	-	chr8_4:27812700-27812719	Msa1321680:three_prime_UTR	30.0%
AGGGGGAAAATTGAAAATTT+GGG	+	chr8_4:27813050-27813069	None:intergenic	30.0%
AAGGGGGAAAATTGAAAATT+TGG	+	chr8_4:27813051-27813070	None:intergenic	30.0%
TGAAGGAAAATGGAATGAAA+GGG	+	chr8_4:27813076-27813095	None:intergenic	30.0%
ATGAAGGAAAATGGAATGAA+AGG	+	chr8_4:27813077-27813096	None:intergenic	30.0%
CTTGAGAAAATGAAGGAAAA+TGG	+	chr8_4:27813086-27813105	None:intergenic	30.0%
TCTTATCTAATGTAAGACTC+TGG	-	chr8_4:27814076-27814095	Msa1321680:intron	30.0%
CTTATCTAATGTAAGACTCT+GGG	-	chr8_4:27814077-27814096	Msa1321680:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	27812504	27817437	27812504	ID=Msa1321680;Name=Msa1321680
chr8_4	mRNA	27812504	27817437	27812504	ID=Msa1321680-mRNA-1;Parent=Msa1321680;Name=Msa1321680-mRNA-1;_AED=0.07;_eAED=0.07;_QI=510\|1\|1\|1\|0.5\|0.33\|3\|498\|106
chr8_4	exon	27812504	27813134	27812504	ID=Msa1321680-mRNA-1:exon:9375;Parent=Msa1321680-mRNA-1
chr8_4	exon	27816575	27816781	27816575	ID=Msa1321680-mRNA-1:exon:9374;Parent=Msa1321680-mRNA-1
chr8_4	exon	27816947	27817437	27816947	ID=Msa1321680-mRNA-1:exon:9373;Parent=Msa1321680-mRNA-1
chr8_4	five_prime_UTR	27816947	27817437	27816947	ID=Msa1321680-mRNA-1:five_prime_utr;Parent=Msa1321680-mRNA-1
chr8_4	five_prime_UTR	27816763	27816781	27816763	ID=Msa1321680-mRNA-1:five_prime_utr;Parent=Msa1321680-mRNA-1
chr8_4	CDS	27816575	27816762	27816575	ID=Msa1321680-mRNA-1:cds;Parent=Msa1321680-mRNA-1
chr8_4	CDS	27813002	27813134	27813002	ID=Msa1321680-mRNA-1:cds;Parent=Msa1321680-mRNA-1
chr8_4	three_prime_UTR	27812504	27813001	27812504	ID=Msa1321680-mRNA-1:three_prime_utr;Parent=Msa1321680-mRNA-1

Gene Sequence	>Msa1321680 ATGTCGAGTGCTGTAGATGCTGCTGGAAACCCGATCCCAACATCAGCGGTGTTGATGGCATCTTCGAAGCACATTGGGTTAAGATGTCATTCGGAGAATTTGGAGTTTCTGAAATGTAAGAAGAAAGATCAGAATCCTGAAAAGTGTCTTGATAAGGGACGTGATGTTACTCGCTGTGTCCTTGGACTTCTCAAGGATCTACACCAAAAGTGCACAAAGGAGATGGATGATTACGTCGGTTGCATGTACTACCATACAAATGAATTCGACTTATGCCGCAAAGAGCAGGAAGCATTTGAGAAAAAATGTTCTTTGGAATGA
Protein sequence	>Msa1321680 MSSAVDAAGNPIPTSAVLMASSKHIGLRCHSENLEFLKCKKKDQNPEKCLDKGRDVTRCVLGLLKDLHQKCTKEMDDYVGCMYYHTNEFDLCRKEQEAFEKKCSLE*