AlfalfaGEDB | Msa1323000

M. sativa cultivar zhongmu-4 / Msa1323000

KEGG: -
GO: -
PFAM: PF00407.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323000	XP_013445246.1	75.373	134	15	2	1	116	23	156	4.64e-63	199

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323000	I3SJ62	75.373	134	15	2	1	116	23	156	2.22e-63	199

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0094100	Msa1323000	0.817378	3.401654e-52	-8.615850e-47
Msa0208630	Msa1323000	0.807201	5.674326e-50	-8.615850e-47
Msa0265820	Msa1323000	0.815025	1.142044e-51	-8.615850e-47
Msa0414950	Msa1323000	-0.823139	1.626562e-53	-8.615850e-47
Msa0442850	Msa1323000	0.822836	1.913843e-53	-8.615850e-47
Msa0480980	Msa1323000	0.807904	4.025231e-50	-8.615850e-47
Msa0533070	Msa1323000	0.808213	3.459554e-50	-8.615850e-47
Msa0540070	Msa1323000	0.800442	1.438260e-48	-8.615850e-47
Msa0581760	Msa1323000	0.801599	8.346704e-49	-8.615850e-47
Msa0638280	Msa1323000	0.808865	2.510024e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323000	MtrunA17_Chr8g0355811	75.373	134	15	2	1	116	23	156	4.27e-67	199
Msa1323000	MtrunA17_Chr8g0355771	74.046	131	16	2	4	116	26	156	8.57e-63	188
Msa1323000	MtrunA17_Chr8g0355791	70.896	134	21	2	1	116	23	156	9.03e-62	185
Msa1323000	MtrunA17_Chr8g0355821	71.212	132	20	2	1	114	23	154	1.72e-60	182
Msa1323000	MtrunA17_Chr8g0355801	70.149	134	22	2	1	116	23	156	5.15e-60	181
Msa1323000	MtrunA17_Chr8g0355721	58.519	135	36	4	1	116	23	156	3.44e-49	154
Msa1323000	MtrunA17_Chr8g0355741	59.259	135	36	3	1	116	23	157	1.54e-48	152
Msa1323000	MtrunA17_Chr8g0355781	58.462	130	35	3	1	111	23	152	3.39e-47	148
Msa1323000	MtrunA17_Chr8g0355661	54.074	135	42	4	1	116	23	156	4.56e-45	143
Msa1323000	MtrunA17_Chr8g0355641	52.239	134	45	3	1	115	23	156	2.12e-44	141

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323000	AT1G70870.1	39.815	108	61	4	6	110	32	138	5.33e-17	72.0
Msa1323000	AT1G24020.1	36.047	86	52	3	27	110	67	151	7.77e-11	56.2
Msa1323000	AT1G24020.2	36.047	86	52	3	27	110	67	151	7.77e-11	56.2

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Find 19 sgRNAs with CRISPR-Local

Find 585 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCACTTTCAAGCTGTT+TGG	0.341180	8_4:-30600380	Msa1323000:CDS
AAATGCACTAGAGATATTGA+TGG	0.497526	8_4:-30600212	Msa1323000:CDS
ATCACTTTCAAGCTGTTTGG+TGG	0.445555	8_4:-30600377	Msa1323000:CDS
ATGGATGTTCTGCTGTTAGA+TGG	0.411936	8_4:-30600298	Msa1323000:CDS
ATGTCGATACTCCAAATGGA+TGG	0.591101	8_4:-30600247	Msa1323000:CDS
ATTGATGGTCATCTTGTCAA+GGG	0.586933	8_4:-30600197	Msa1323000:CDS
CATCATGGTAAGCTTCACGA+AGG	0.725628	8_4:-30606330	Msa1323000:intron
CGATACTCCAAATGGATGGA+TGG	0.564408	8_4:-30600243	Msa1323000:CDS
CTGTAGTCCATCCATCCATT+TGG	0.363838	8_4:+30600236	None:intergenic
GAAGTGATTAACAAGGCTGA+TGG	0.565494	8_4:-30600317	Msa1323000:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTGGAAACATAGTTACCTA+TGG	+	chr8_4:30600362-30600381	None:intergenic	30.0%
AATGTGAACAACATTAGATG+AGG	+	chr8_4:30600892-30600911	None:intergenic	30.0%
AGTGAAAAGATACAGAAGAA+GGG	+	chr8_4:30601593-30601612	None:intergenic	30.0%
AAGTGAAAAGATACAGAAGA+AGG	+	chr8_4:30601594-30601613	None:intergenic	30.0%
TTATGGAAGAATGTTACGAA+CGG	+	chr8_4:30601621-30601640	None:intergenic	30.0%
TAACATTCTTCCATAATCAC+TGG	-	chr8_4:30601625-30601644	Msa1323000:intron	30.0%
AAGTATATCAGCTTCATAGA+TGG	-	chr8_4:30601714-30601733	Msa1323000:intron	30.0%
AAAATAGAGAATGAATGCGA+GGG	-	chr8_4:30601799-30601818	Msa1323000:intron	30.0%
TGGAATAGAAGGAGAAATTA+TGG	-	chr8_4:30601918-30601937	Msa1323000:intron	30.0%
ACACAAAGAGAACATAACAA+CGG	-	chr8_4:30602138-30602157	Msa1323000:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	30600178	30606466	30600178	ID=Msa1323000;Name=Msa1323000
chr8_4	mRNA	30600178	30606466	30600178	ID=Msa1323000-mRNA-1;Parent=Msa1323000;Name=Msa1323000-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0\|0\|0\|1\|0\|0\|3\|0\|116
chr8_4	exon	30606454	30606466	30606454	ID=Msa1323000-mRNA-1:exon:10156;Parent=Msa1323000-mRNA-1
chr8_4	exon	30606331	30606387	30606331	ID=Msa1323000-mRNA-1:exon:10155;Parent=Msa1323000-mRNA-1
chr8_4	exon	30600178	30600458	30600178	ID=Msa1323000-mRNA-1:exon:10154;Parent=Msa1323000-mRNA-1
chr8_4	CDS	30606454	30606466	30606454	ID=Msa1323000-mRNA-1:cds;Parent=Msa1323000-mRNA-1
chr8_4	CDS	30606331	30606387	30606331	ID=Msa1323000-mRNA-1:cds;Parent=Msa1323000-mRNA-1
chr8_4	CDS	30600178	30600458	30600178	ID=Msa1323000-mRNA-1:cds;Parent=Msa1323000-mRNA-1

Gene Sequence	>Msa1323000 ATGGTGCTCGCTGAACTTCACGAAGTGCAAAACGTTTGTGAAAGAGTTCATCATGGTAAGCTTCACGAAGATGGCGAGGTACACACATGCAATGAGACTGTCGAAGAAGTTGATGATCAGAACAGAAAAATCACTTTCAAGCTGTTTGGTGGAGATATTGAAAACTATAAGGTCTTCAAGGCTATCTTAGAAGTGATTAACAAGGCTGATGGATGTTCTGCTGTTAGATGGAGTATTGATTATGAGAAGAAAAATGAGGATGTCGATACTCCAAATGGATGGATGGACTACAGCAAATGCACTAGAGATATTGATGGTCATCTTGTCAAGGGGGAAAAGGTAGCTCTATAG
Protein sequence	>Msa1323000 MVLAELHEVQNVCERVHHGKLHEDGEVHTCNETVEEVDDQNRKITFKLFGGDIENYKVFKAILEVINKADGCSAVRWSIDYEKKNEDVDTPNGWMDYSKCTRDIDGHLVKGEKVAL*