AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1334140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1334140 MtrunA17_Chr4g0061881 45.000 540 248 13 75 582 67 589 8.46e-137 415
Msa1334140 MtrunA17_Chr2g0307851 41.808 531 271 9 79 583 84 602 7.41e-127 389
Msa1334140 MtrunA17_Chr4g0069071 39.628 376 207 6 130 494 5 371 4.22e-74 242
Msa1334140 MtrunA17_Chr4g0073611 35.197 304 160 8 149 445 2 275 3.15e-46 165
Msa1334140 MtrunA17_Chr5g0408331 31.856 361 159 12 76 429 32 312 4.29e-40 149
Msa1334140 MtrunA17_Chr4g0061841 33.660 306 110 8 148 442 8 231 2.90e-37 139
Msa1334140 MtrunA17_Chr4g0076561 37.255 204 100 3 337 539 10 186 4.07e-35 131
Msa1334140 MtrunA17_Chr5g0437391 30.460 348 179 9 210 553 12 300 1.58e-34 134
Msa1334140 MtrunA17_Chr8g0344851 41.885 191 95 6 411 594 1 182 2.25e-34 129
Msa1334140 MtrunA17_Chr4g0061831 52.294 109 51 1 473 581 9 116 2.18e-32 121
Msa1334140 MtrunA17_Chr4g0064001 43.537 147 68 3 436 582 4 135 1.60e-25 105
Msa1334140 MtrunA17_Chr8g0344821 44.444 99 53 1 484 582 11 107 2.58e-21 89.7
Msa1334140 MtrunA17_Chr4g0076571 55.128 78 26 1 144 221 1 69 5.48e-21 88.2
Msa1334140 MtrunA17_Chr5g0437441 52.222 90 37 3 107 193 7 93 1.57e-20 87.0
Msa1334140 MtrunA17_Chr8g0344831 43.750 112 58 2 326 432 7 118 2.45e-18 81.3
Msa1334140 MtrunA17_Chr4g0069081 47.500 80 42 0 506 585 1 80 1.21e-16 76.3
Msa1334140 MtrunA17_Chr5g0437451 35.507 138 68 4 443 578 30 148 1.61e-16 77.4
Msa1334140 MtrunA17_Chr4g0073621 61.017 59 21 1 526 582 1 59 3.18e-16 73.9
Msa1334140 MtrunA17_Chr5g0408341 63.462 52 19 0 531 582 1 52 3.04e-14 70.1
Msa1334140 MtrunA17_Chr4g0063991 49.254 67 33 1 338 403 1 67 2.03e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1334140 AT1G73990.1 38.475 590 320 13 22 582 32 607 6.18e-119 369
Msa1334140 AT1G73990.2 36.059 538 301 13 22 530 32 555 4.15e-93 300

Find 177 sgRNAs with CRISPR-Local

Find 303 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGATTTCGTCCCGCATTC+TGG 0.209844 8_4:-54457975 Msa1334140:CDS
GAAGATGAGCGGATCATAAA+TGG 0.215556 8_4:-54456727 Msa1334140:CDS
TGTGCTAAGAGCTTGCTTTA+AGG 0.216377 8_4:+54454187 None:intergenic
GCTTGACTCTGCTGTTTAAA+TGG 0.219189 8_4:-54454350 Msa1334140:three_prime_UTR
ATAGATTGTAGGTTACTTTC+AGG 0.236540 8_4:-54457291 Msa1334140:CDS
GATAGGCAAAATCACATTAT+AGG 0.245255 8_4:+54454044 None:intergenic
TTGTGAATGTGAGGGGTTTC+GGG 0.245290 8_4:+54458146 None:intergenic
TTTGTGAATGTGAGGGGTTT+CGG 0.257624 8_4:+54458145 None:intergenic
TATTAGCATGTAGATGATTT+AGG 0.257669 8_4:-54454444 Msa1334140:three_prime_UTR
TTGGTATAAGTACCCATTTC+GGG 0.263338 8_4:+54454583 None:intergenic
CTCACTTATACATGTCAAAT+TGG 0.274908 8_4:-54456877 Msa1334140:CDS
CCATTGGTTGATGTGCATTC+AGG 0.286389 8_4:-54454519 Msa1334140:CDS
GAGAGTTCAACCAGATTAAT+AGG 0.304701 8_4:+54456505 None:intergenic
AACAGCTCCTTCTTCCAATC+AGG 0.317463 8_4:+54454559 None:intergenic
AGATTGCCCAAGGGCGGCTT+TGG 0.320112 8_4:-54456630 Msa1334140:CDS
TACTTAGACATGACTTAGTT+TGG 0.326609 8_4:-54454288 Msa1334140:three_prime_UTR
ATGACTTAGTTTGGCCAAAC+TGG 0.336189 8_4:-54454279 Msa1334140:three_prime_UTR
TTGTATAACTTCTCAGTGTT+TGG 0.338375 8_4:+54456838 None:intergenic
TAGGTTACTTTCAGGGTTGA+TGG 0.343659 8_4:-54457283 Msa1334140:CDS
AGGTATATTAGCCTTCAATT+TGG 0.345021 8_4:+54456537 None:intergenic
CATCACTGTTCAAATTTGAT+AGG 0.347256 8_4:+54454027 None:intergenic
GGGCTATCAATACTTGTATC+TGG 0.347744 8_4:+54457543 None:intergenic
TAGAAAATTGGATAGATTGT+AGG 0.350787 8_4:-54457302 Msa1334140:CDS
GTGCTAAGAGCTTGCTTTAA+GGG 0.353149 8_4:+54454188 None:intergenic
TAACAGAAGGGAAGCCAGTT+TGG 0.364170 8_4:+54454265 None:intergenic
ACCAGTTGGCACTGACTCTA+AGG 0.365682 8_4:-54457850 Msa1334140:CDS
TGTAGATGATTTAGGCCATT+TGG 0.369425 8_4:-54454436 Msa1334140:three_prime_UTR
TCCCGGTCCAAAGCCGCCCT+TGG 0.369925 8_4:+54456623 None:intergenic
TTGTTATGGTTTCTTCTTCA+AGG 0.372590 8_4:-54457380 Msa1334140:CDS
ATTGAGATTATCCGTGGGGC+TGG 0.376386 8_4:-54457093 Msa1334140:CDS
ATCGGTGTTATTAGAGTTTG+TGG 0.378619 8_4:-54457147 Msa1334140:CDS
CTGGTATGATTTGTTTGTCC+TGG 0.379188 8_4:-54453984 Msa1334140:three_prime_UTR
AAGATGTAACTGGCTTGTTA+TGG 0.382012 8_4:-54457394 Msa1334140:CDS
TCTTCTTCCACTTCAGGTAA+TGG 0.382352 8_4:-54458026 Msa1334140:CDS
ATTGTCGTGAAGTAGAAAAT+TGG 0.383838 8_4:-54457314 Msa1334140:CDS
TCATCTTCATGTGGCATGAT+TGG 0.388034 8_4:+54456742 None:intergenic
ACGGGTCTCAGTAGGAGATT+TGG 0.389865 8_4:-54457243 Msa1334140:CDS
GCCCAAGGGCGGCTTTGGAC+CGG 0.392493 8_4:-54456625 Msa1334140:CDS
TCAAAATAGACCTATTAATC+TGG 0.409814 8_4:-54456515 Msa1334140:CDS
AAACTGGACAAAGCCCGAAA+TGG 0.415642 8_4:-54454596 Msa1334140:CDS
GTTGGTATAAGTACCCATTT+CGG 0.416903 8_4:+54454582 None:intergenic
CTTATCAGAACACAACAAAC+TGG 0.418408 8_4:-54454612 Msa1334140:CDS
CTCCTCCTGGACTGTCAATC+CGG 0.422871 8_4:+54457040 None:intergenic
AAGTAAAGATCGATGACAAT+AGG 0.426110 8_4:+54454306 None:intergenic
CCCAAGGGCGGCTTTGGACC+GGG 0.429403 8_4:-54456624 Msa1334140:CDS
TATGTTTGTGCATTATTGTC+TGG 0.433909 8_4:-54454003 Msa1334140:three_prime_UTR
GATGTGCATTCAGGAATAAC+AGG 0.437794 8_4:-54454510 Msa1334140:CDS
GGTGCATATTTGATTGCAAC+TGG 0.441687 8_4:-54456925 Msa1334140:CDS
CCCGGTCCAAAGCCGCCCTT+GGG 0.444189 8_4:+54456624 None:intergenic
GTAAAACAACAGTCCTTTGA+AGG 0.445649 8_4:+54454634 None:intergenic
GAAGTTTACAAAGTCTTTGT+GGG 0.446335 8_4:-54456697 Msa1334140:CDS
AACTGGACAAAGCCCGAAAT+GGG 0.447850 8_4:-54454595 Msa1334140:CDS
GATCAAATGCGGCTTTCATA+AGG 0.449687 8_4:+54457799 None:intergenic
GGAATCGATGAAGGCAGTTA+AGG 0.451737 8_4:-54457424 Msa1334140:CDS
TATTTGATTGCAACTGGAGC+AGG 0.455192 8_4:-54456919 Msa1334140:CDS
AACACGGTCCACAATGGAAA+TGG 0.473603 8_4:+54458094 None:intergenic
TAAATACAATCCGTGTTCAC+AGG 0.476299 8_4:+54457522 None:intergenic
TAGTGTGCGGTAGAATTTGA+GGG 0.478545 8_4:+54458121 None:intergenic
CACATCTACACTTAACGATC+TGG 0.479653 8_4:+54456660 None:intergenic
GACGAAATCAAACTCACCCT+TGG 0.479725 8_4:+54457986 None:intergenic
ACCTTAGAGTCAGTGCCAAC+TGG 0.479839 8_4:+54457849 None:intergenic
ATGAATTGCACCTTCATTGT+GGG 0.482660 8_4:+54457122 None:intergenic
AGAAGTTTACAAAGTCTTTG+TGG 0.483301 8_4:-54456698 Msa1334140:CDS
ACCGTTACCATTACCTGAAG+TGG 0.483377 8_4:+54458019 None:intergenic
TTGAGATTATCCGTGGGGCT+GGG 0.490077 8_4:-54457092 Msa1334140:CDS
CTTCAAGAAATTTATTGTGA+AGG 0.493765 8_4:-54457952 Msa1334140:CDS
CTATCTTCACACCATAATAC+AGG 0.496409 8_4:+54457633 None:intergenic
ATCTTGTTGAAGTGCAGTAT+AGG 0.498853 8_4:-54457494 Msa1334140:CDS
AACTAGCATATAAAACCAAA+TGG 0.499171 8_4:+54454421 None:intergenic
TAGATTGTAGGTTACTTTCA+GGG 0.501518 8_4:-54457290 Msa1334140:CDS
GTACTTATACCAACCCTGAT+TGG 0.505029 8_4:-54454573 Msa1334140:CDS
CTTCTGTTAAAGCAAGCTCT+TGG 0.507466 8_4:-54454251 Msa1334140:three_prime_UTR
ATAGTGTGCGGTAGAATTTG+AGG 0.507587 8_4:+54458120 None:intergenic
CTTGTATCTGGAGAATCATC+CGG 0.508155 8_4:+54457555 None:intergenic
AGGATGATATAAGATGTAAC+TGG 0.510806 8_4:-54457404 Msa1334140:CDS
GAAGTGCAGTATAGGACAGC+GGG 0.512732 8_4:-54457486 Msa1334140:CDS
TGTTGCTATAGCCAAATTGA+AGG 0.514181 8_4:-54456548 Msa1334140:CDS
ACAGCGGGGAAATACAAATC+TGG 0.516542 8_4:-54457471 Msa1334140:CDS
CAAGAGCTTGCTTTAACAGA+AGG 0.519724 8_4:+54454252 None:intergenic
CAAGCGCGTCAACCAACATA+TGG 0.522040 8_4:+54456587 None:intergenic
ATGTTGGTTGACGCGCTTGG+TGG 0.522308 8_4:-54456583 Msa1334140:CDS
CGTTTCTTCATTATCAGACA+AGG 0.522976 8_4:-54456956 Msa1334140:CDS
TGCACTGTTGGTATGTCAAA+TGG 0.523328 8_4:-54457180 Msa1334140:CDS
CTCCTTCTTCCAATCAGGGT+TGG 0.525262 8_4:+54454564 None:intergenic
ATTTCTTGAAGCCAGAATGC+GGG 0.525455 8_4:+54457964 None:intergenic
TCAACATCAGTGTACTCCAT+TGG 0.529318 8_4:-54454535 Msa1334140:CDS
ACGAAATCAAACTCACCCTT+GGG 0.529474 8_4:+54457987 None:intergenic
GTTTGAAGGCTGTAATCCTC+CGG 0.530693 8_4:-54457059 Msa1334140:CDS
TTACAAGGAAATATTTCCGA+TGG 0.530898 8_4:-54457873 Msa1334140:CDS
TTACAAAGTCTTTGTGGGCA+AGG 0.531876 8_4:-54456692 Msa1334140:CDS
AAGATGAGCGGATCATAAAT+GGG 0.535057 8_4:-54456726 Msa1334140:CDS
TAAATATGCAGAATTATTTG+AGG 0.535075 8_4:-54456776 Msa1334140:CDS
GTCAGTGCCAACTGGTCCAT+CGG 0.538824 8_4:+54457857 None:intergenic
TGAAGGATGGGAATCGATGA+AGG 0.539636 8_4:-54457433 Msa1334140:CDS
GGACATTTAACCACAGATGA+AGG 0.540268 8_4:-54457450 Msa1334140:CDS
ACTATCTTCCATTTCCATTG+TGG 0.545148 8_4:-54458102 Msa1334140:CDS
GTTGTTTGAGATGAAGATGA+AGG 0.549690 8_4:+54458190 None:intergenic
GCCTCGGAGCATGTGGAACA+TGG 0.549933 8_4:-54457915 Msa1334140:CDS
CTCAAAACAGTATCAAATCC+AGG 0.550202 8_4:+54454377 None:intergenic
AGATTATCACGGGTCTCAGT+AGG 0.550801 8_4:-54457251 Msa1334140:CDS
CATGAATTGCACCTTCATTG+TGG 0.551816 8_4:+54457121 None:intergenic
GCGGCTTTGGACCGGGAAGG+CGG 0.553063 8_4:-54456617 Msa1334140:CDS
ACTAACGTTGGTTCTTGAGT+AGG 0.554396 8_4:+54458056 None:intergenic
TTCAAGGAAAATAAGAGAAG+AGG 0.555199 8_4:-54457364 Msa1334140:CDS
GTTTGTGGATCCCACAATGA+AGG 0.556262 8_4:-54457132 Msa1334140:CDS
AGGGCGGCTTTGGACCGGGA+AGG 0.558400 8_4:-54456620 Msa1334140:CDS
GATTTGATTGAGATTATCCG+TGG 0.559731 8_4:-54457099 Msa1334140:CDS
CTGTTGGTATGTCAAATGGC+AGG 0.562620 8_4:-54457176 Msa1334140:CDS
TCCACTTCAGGTAATGGTAA+CGG 0.563991 8_4:-54458020 Msa1334140:CDS
TCTGTTAGCCTCGGAGCATG+TGG 0.564327 8_4:-54457922 Msa1334140:CDS
ACAGGTTCACAGATAGCTCC+AGG 0.568791 8_4:+54453966 None:intergenic
TCTGATAATGAAGAAACGAC+TGG 0.568882 8_4:+54456961 None:intergenic
ATTTAACCACAGATGAAGGA+TGG 0.568911 8_4:-54457446 Msa1334140:CDS
GAGAAGTTATACAAATACAT+TGG 0.571158 8_4:-54456829 Msa1334140:CDS
ATAACACAGTTCAAAACTGA+TGG 0.572477 8_4:+54454100 None:intergenic
TACCAACCCTGATTGGAAGA+AGG 0.572792 8_4:-54454566 Msa1334140:CDS
GGGGTTTCGGGAAGATGAAG+AGG 0.574598 8_4:+54458158 None:intergenic
AAATACAATCCGTGTTCACA+GGG 0.575360 8_4:+54457523 None:intergenic
ATTACTAATGTAGAGAGTGA+AGG 0.576126 8_4:+54454150 None:intergenic
TTCAGGAATAACAGGAGGGA+TGG 0.577523 8_4:-54454502 Msa1334140:CDS
ATATTGAGAATCAATTTACA+AGG 0.578147 8_4:-54457888 Msa1334140:CDS
TGAGGGATTTGTGAATGTGA+GGG 0.580001 8_4:+54458138 None:intergenic
GCTAGTGATAAGATTATCAC+GGG 0.582665 8_4:-54457261 Msa1334140:CDS
ACAGCTCCTTCTTCCAATCA+GGG 0.583747 8_4:+54454560 None:intergenic
GTGGTTGACGAGATTGCCCA+AGG 0.585641 8_4:-54456640 Msa1334140:CDS
AATTATTTGAGGCTGATCAT+CGG 0.586001 8_4:-54456765 Msa1334140:CDS
TTGACGAGATTGCCCAAGGG+CGG 0.586860 8_4:-54456636 Msa1334140:CDS
ATCCTCCGGATTGACAGTCC+AGG 0.587117 8_4:-54457045 Msa1334140:CDS
TATTGATAGCCCTGTGAACA+CGG 0.587964 8_4:-54457532 Msa1334140:CDS
CATATGTTGGTTGACGCGCT+TGG 0.589554 8_4:-54456586 Msa1334140:CDS
CTTTGTGGGCAAGGTAGCGA+TGG 0.589923 8_4:-54456683 Msa1334140:CDS
AAGAGCTTGCTTTAACAGAA+GGG 0.590552 8_4:+54454253 None:intergenic
TGGATGTTGTATGCCTTCAA+AGG 0.592887 8_4:-54454647 Msa1334140:CDS
CGATTCCCATCCTTCATCTG+TGG 0.594942 8_4:+54457440 None:intergenic
GGCGGCACTGAACCATATGT+TGG 0.595357 8_4:-54456599 Msa1334140:CDS
GGCTAGTGATAAGATTATCA+CGG 0.596190 8_4:-54457262 Msa1334140:CDS
GGTTAAGACTCTGTTAGCCT+CGG 0.596334 8_4:-54457931 Msa1334140:CDS
AATATTTCCGATGGACCAGT+TGG 0.597583 8_4:-54457864 Msa1334140:CDS
TCTGGAGAATCATCCGGAGG+GGG 0.598601 8_4:+54457561 None:intergenic
TTTAACCACAGATGAAGGAT+GGG 0.605296 8_4:-54457445 Msa1334140:CDS
TTGAGGGATTTGTGAATGTG+AGG 0.606072 8_4:+54458137 None:intergenic
GAGGGATTTGTGAATGTGAG+GGG 0.607039 8_4:+54458139 None:intergenic
CTCCTGGACTGTCAATCCGG+AGG 0.612922 8_4:+54457043 None:intergenic
TGTTGGTATGTCAAATGGCA+GGG 0.616785 8_4:-54457175 Msa1334140:CDS
AATTTCTTGAAGCCAGAATG+CGG 0.621440 8_4:+54457963 None:intergenic
TTCAAAAGTTGACAGAGTGA+AGG 0.622058 8_4:+54454067 None:intergenic
TGCTGCAACACGGTCCACAA+TGG 0.622831 8_4:+54458088 None:intergenic
TATCTGGAGAATCATCCGGA+GGG 0.623341 8_4:+54457559 None:intergenic
TCAAATGGCAGGGAAAAGAT+CGG 0.628546 8_4:-54457165 Msa1334140:CDS
AAGGAGGTTCTTCACAGAGG+TGG 0.630578 8_4:-54456799 Msa1334140:CDS
GAAGATGAAAACACTAACGT+TGG 0.631994 8_4:+54458044 None:intergenic
ATGATCCGCTCATCTTCATG+TGG 0.633308 8_4:+54456733 None:intergenic
ATCTGGAGAATCATCCGGAG+GGG 0.638622 8_4:+54457560 None:intergenic
TTATCAGACAAGGCAACTAG+TGG 0.639169 8_4:-54456946 Msa1334140:CDS
GTGCATTCAGGAATAACAGG+AGG 0.649237 8_4:-54454507 Msa1334140:CDS
CCTGAATGCACATCAACCAA+TGG 0.649333 8_4:+54454519 None:intergenic
ATAATAAACTCAACTCACAC+AGG 0.658801 8_4:+54453948 None:intergenic
CAGATCGTTAAGTGTAGATG+TGG 0.659544 8_4:-54456659 Msa1334140:CDS
GTATCTGGAGAATCATCCGG+AGG 0.659830 8_4:+54457558 None:intergenic
ATTTGATTGAGATTATCCGT+GGG 0.666741 8_4:-54457098 Msa1334140:CDS
AGAAACACAGCGGACACACG+AGG 0.666748 8_4:+54457777 None:intergenic
TGCATTCAGGAATAACAGGA+GGG 0.668569 8_4:-54454506 Msa1334140:CDS
TTTCGGGAAGATGAAGAGGA+TGG 0.668798 8_4:+54458162 None:intergenic
TCATGCCACATGAAGATGAG+CGG 0.671782 8_4:-54456738 Msa1334140:CDS
AAGAAGGAGGTTCTTCACAG+AGG 0.681303 8_4:-54456802 Msa1334140:CDS
CTCCGGATTGACAGTCCAGG+AGG 0.681617 8_4:-54457042 Msa1334140:CDS
TGGTTGACGAGATTGCCCAA+GGG 0.693802 8_4:-54456639 Msa1334140:CDS
GAAGTAAAGATGCTGCAACA+CGG 0.695209 8_4:+54458078 None:intergenic
TCCATGTTCCACATGCTCCG+AGG 0.700945 8_4:+54457914 None:intergenic
CTTTAAGGGAAGCCTAACAG+TGG 0.711846 8_4:+54454202 None:intergenic
TATGCTTTGAAATCGAGCAG+TGG 0.712517 8_4:+54457219 None:intergenic
TGGAAATGGAAGATAGTGTG+CGG 0.715103 8_4:+54458108 None:intergenic
TGAAGTGCAGTATAGGACAG+CGG 0.718438 8_4:-54457487 Msa1334140:CDS
AACTATATTAAGAAACACAG+CGG 0.726464 8_4:+54457767 None:intergenic
AAGTGCAGTATAGGACAGCG+GGG 0.749747 8_4:-54457485 Msa1334140:CDS
TTTGATTGAGATTATCCGTG+GGG 0.755479 8_4:-54457097 Msa1334140:CDS
GGACACACGAGGATCAAATG+CGG 0.763717 8_4:+54457788 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATCAAATTTTAAAAAATC+TGG - chr8_4:54454472-54454491 Msa1334140:exon 10.0%
!!! AATATAGTTGATTTTTATTG+TGG - chr8_4:54454418-54454437 Msa1334140:three_prime_UTR 15.0%
!!! TTTTAAAAAATCTGGTAAAA+AGG - chr8_4:54454480-54454499 Msa1334140:exon 15.0%
!!! AATTTTTTAAAATCAAGGAA+TGG + chr8_4:54457151-54457170 None:intergenic 15.0%
!!! ATTCCTTGATTTTAAAAAAT+TGG - chr8_4:54457150-54457169 Msa1334140:CDS 15.0%
!! ATATTGAGAATCAATTTACA+AGG - chr8_4:54454283-54454302 Msa1334140:three_prime_UTR 20.0%
!!! TAAAAAGGTTATAGCTTTTA+TGG - chr8_4:54454495-54454514 Msa1334140:CDS 20.0%
!! TAAATATGCAGAATTATTTG+AGG - chr8_4:54455395-54455414 Msa1334140:intron 20.0%
!!! ACTATTTTTCTTTCTTTTAC+TGG - chr8_4:54455722-54455741 Msa1334140:intron 20.0%
!! GAAAAAAACCTTACAAAATT+GGG + chr8_4:54455856-54455875 None:intergenic 20.0%
!! AGAAAAAAACCTTACAAAAT+TGG + chr8_4:54455857-54455876 None:intergenic 20.0%
!!! TTTGTAAGGTTTTTTTCTTT+TGG - chr8_4:54455859-54455878 Msa1334140:intron 20.0%
!! AATTTAATATTCTTCCTTCA+AGG - chr8_4:54456205-54456224 Msa1334140:intron 20.0%
!!! TTTTTAAAGTAATTAGCGTA+TGG + chr8_4:54456522-54456541 None:intergenic 20.0%
!! AAAAACATAGAATTCAAAAC+AGG + chr8_4:54456757-54456776 None:intergenic 20.0%
!!! GAACCAATTTTTTAAAATCA+AGG + chr8_4:54457156-54457175 None:intergenic 20.0%
!!! AATTTTCTAAAATCAAGGAA+TGG + chr8_4:54457407-54457426 None:intergenic 20.0%
!!! ATTCCTTGATTTTAGAAAAT+TGG - chr8_4:54457406-54457425 Msa1334140:CDS 20.0%
!!! GTTTGTAAATTTTAGACTTA+TGG - chr8_4:54457471-54457490 Msa1334140:CDS 20.0%
! CTTCAAGAAATTTATTGTGA+AGG - chr8_4:54454219-54454238 Msa1334140:three_prime_UTR 25.0%
! AACTATATTAAGAAACACAG+CGG + chr8_4:54454407-54454426 None:intergenic 25.0%
!!! TAGTTGATTTTTATTGTGGT+TGG - chr8_4:54454422-54454441 Msa1334140:three_prime_UTR 25.0%
!!! AGTTGATTTTTATTGTGGTT+GGG - chr8_4:54454423-54454442 Msa1334140:three_prime_UTR 25.0%
! TAGAAAATTGGATAGATTGT+AGG - chr8_4:54454869-54454888 Msa1334140:intron 25.0%
! GAGAAGTTATACAAATACAT+TGG - chr8_4:54455342-54455361 Msa1334140:intron 25.0%
!!! CAAATACATTGGTTTTAAGA+AGG - chr8_4:54455353-54455372 Msa1334140:intron 25.0%
!! AGATTAATAGGTCTATTTTG+AGG + chr8_4:54455657-54455676 None:intergenic 25.0%
! TCAAAATAGACCTATTAATC+TGG - chr8_4:54455656-54455675 Msa1334140:intron 25.0%
!!! AACTTTTTCCCAATTTTGTA+AGG - chr8_4:54455845-54455864 Msa1334140:intron 25.0%
! GGGAAAAAACAAACAAAAAA+GGG + chr8_4:54456034-54456053 None:intergenic 25.0%
!!! ATTTTCTCTGAGAACTAAAA+AGG - chr8_4:54456315-54456334 Msa1334140:intron 25.0%
! TGAAATAACACTATAATGAC+CGG + chr8_4:54456705-54456724 None:intergenic 25.0%
!!! TTACCAGTAATTTTTTTGCT+AGG - chr8_4:54457096-54457115 Msa1334140:CDS 25.0%
! AATCCTAGCAAAAAAATTAC+TGG + chr8_4:54457102-54457121 None:intergenic 25.0%
!!! AGTAATTTTTTTGCTAGGAT+TGG - chr8_4:54457101-54457120 Msa1334140:CDS 25.0%
!!! TTACCAGTAATTTTTTTGCT+AGG - chr8_4:54457096-54457115 Msa1334140:CDS 25.0%
! AATCCTAGCAAAAAAATTAC+TGG + chr8_4:54457102-54457121 None:intergenic 25.0%
!!! AGTAATTTTTTTGCTAGGAT+TGG - chr8_4:54457101-54457120 Msa1334140:CDS 25.0%
!!! GAACCAATTTTCTAAAATCA+AGG + chr8_4:54457412-54457431 None:intergenic 25.0%
! AAAATTTACAAACCAGAAAC+CGG + chr8_4:54457464-54457483 None:intergenic 25.0%
! TGCAGAAAAAAGAAAACAAA+TGG + chr8_4:54457501-54457520 None:intergenic 25.0%
! TATTAGCATGTAGATGATTT+AGG - chr8_4:54457727-54457746 Msa1334140:CDS 25.0%
! AACTAGCATATAAAACCAAA+TGG + chr8_4:54457753-54457772 None:intergenic 25.0%
!!! TATATGCTAGTTTTTTAGTG+TGG - chr8_4:54457761-54457780 Msa1334140:CDS 25.0%
!!! ATATGCTAGTTTTTTAGTGT+GGG - chr8_4:54457762-54457781 Msa1334140:CDS 25.0%
TTACAAGGAAATATTTCCGA+TGG - chr8_4:54454298-54454317 Msa1334140:three_prime_UTR 30.0%
! TAGCTTTTATGGCTTCATTT+TGG - chr8_4:54454506-54454525 Msa1334140:CDS 30.0%
AGGATGATATAAGATGTAAC+TGG - chr8_4:54454767-54454786 Msa1334140:intron 30.0%
TTGTTATGGTTTCTTCTTCA+AGG - chr8_4:54454791-54454810 Msa1334140:intron 30.0%
TTCAAGGAAAATAAGAGAAG+AGG - chr8_4:54454807-54454826 Msa1334140:intron 30.0%
!!! GAGGAGATTCTAACTTTTTT+TGG - chr8_4:54454826-54454845 Msa1334140:intron 30.0%
!!! TCTAACTTTTTTTGGTAGTG+AGG - chr8_4:54454834-54454853 Msa1334140:intron 30.0%
ATTGTCGTGAAGTAGAAAAT+TGG - chr8_4:54454857-54454876 Msa1334140:intron 30.0%
! ATAGATTGTAGGTTACTTTC+AGG - chr8_4:54454880-54454899 Msa1334140:intron 30.0%
! TAGATTGTAGGTTACTTTCA+GGG - chr8_4:54454881-54454900 Msa1334140:intron 30.0%
CAAGTTAAAAAATGCACTGT+TGG - chr8_4:54454979-54454998 Msa1334140:intron 30.0%
ATTTGATTGAGATTATCCGT+GGG - chr8_4:54455073-54455092 Msa1334140:intron 30.0%
!! TTTTGTTGGCTGATCAATTA+TGG - chr8_4:54455157-54455176 Msa1334140:intron 30.0%
CTCACTTATACATGTCAAAT+TGG - chr8_4:54455294-54455313 Msa1334140:intron 30.0%
TTGTATAACTTCTCAGTGTT+TGG + chr8_4:54455336-54455355 None:intergenic 30.0%
!!! ATACATTGGTTTTAAGAAGG+AGG - chr8_4:54455356-54455375 Msa1334140:intron 30.0%
! AATTATTTGAGGCTGATCAT+CGG - chr8_4:54455406-54455425 Msa1334140:intron 30.0%
AGAAGTTTACAAAGTCTTTG+TGG - chr8_4:54455473-54455492 Msa1334140:intron 30.0%
GAAGTTTACAAAGTCTTTGT+GGG - chr8_4:54455474-54455493 Msa1334140:intron 30.0%
!! AGGTATATTAGCCTTCAATT+TGG + chr8_4:54455637-54455656 None:intergenic 30.0%
!! TTTTACTGGTTGAACTCTTT+GGG - chr8_4:54455736-54455755 Msa1334140:intron 30.0%
!!! TATGTTTTTTTGCCGAAAAG+TGG - chr8_4:54455763-54455782 Msa1334140:intron 30.0%
!! TGCATTAAAAGTCCACTTTT+CGG + chr8_4:54455778-54455797 None:intergenic 30.0%
!!! GGTTTTTTTCTTTTGGAAAG+TGG - chr8_4:54455866-54455885 Msa1334140:intron 30.0%
! GACTTTTACATTGGATAGTT+TGG - chr8_4:54455888-54455907 Msa1334140:intron 30.0%
ACGTTCTACAAATGATTCAT+TGG + chr8_4:54456003-54456022 None:intergenic 30.0%
GGGGAAAAAACAAACAAAAA+AGG + chr8_4:54456035-54456054 None:intergenic 30.0%
! AAGCAAAGAGAATTCAAGTT+TGG + chr8_4:54456157-54456176 None:intergenic 30.0%
TTCTCTTTGCTTTGCAATAT+TGG - chr8_4:54456165-54456184 Msa1334140:intron 30.0%
TTGAATGAGATTTCAACAAG+TGG - chr8_4:54456255-54456274 Msa1334140:intron 30.0%
TGAATGAGATTTCAACAAGT+GGG - chr8_4:54456256-54456275 Msa1334140:intron 30.0%
AATAAATGCAAACAAGAGCT+TGG + chr8_4:54456409-54456428 None:intergenic 30.0%
! AGCTCTTGTTTGCATTTATT+TGG - chr8_4:54456409-54456428 Msa1334140:intron 30.0%
GACTTAAACATTCTCAAAAC+TGG + chr8_4:54456453-54456472 None:intergenic 30.0%
! AGTTTTTGTATGACACATAC+CGG - chr8_4:54456683-54456702 Msa1334140:CDS 30.0%
! TTTTATGCTCAACTTGTACA+TGG - chr8_4:54456776-54456795 Msa1334140:CDS 30.0%
! AGGGATACACAAATTTTACA+TGG + chr8_4:54456890-54456909 None:intergenic 30.0%
AATGATATTGTCAACCTTCA+TGG + chr8_4:54456927-54456946 None:intergenic 30.0%
! GCATTGACACTTAAATTGAA+TGG + chr8_4:54457025-54457044 None:intergenic 30.0%
! ACTGTTTGATGTATTTCTGT+CGG - chr8_4:54457179-54457198 Msa1334140:CDS 30.0%
! GCATTGACACTTAAATTGAA+TGG + chr8_4:54457025-54457044 None:intergenic 30.0%
! ACTGTTTGATGTATTTCTGT+CGG - chr8_4:54457179-54457198 Msa1334140:CDS 30.0%
AAGTAAAGATCGATGACAAT+AGG + chr8_4:54457868-54457887 None:intergenic 30.0%
TACTTAGACATGACTTAGTT+TGG - chr8_4:54457883-54457902 Msa1334140:CDS 30.0%
ATTACTAATGTAGAGAGTGA+AGG + chr8_4:54458024-54458043 None:intergenic 30.0%
ATAACACAGTTCAAAACTGA+TGG + chr8_4:54458074-54458093 None:intergenic 30.0%
GATAGGCAAAATCACATTAT+AGG + chr8_4:54458130-54458149 None:intergenic 30.0%
CATCACTGTTCAAATTTGAT+AGG + chr8_4:54458147-54458166 None:intergenic 30.0%
TATGTTTGTGCATTATTGTC+TGG - chr8_4:54458168-54458187 Msa1334140:CDS 30.0%
ATAATAAACTCAACTCACAC+AGG + chr8_4:54458226-54458245 None:intergenic 30.0%
! GTTGTTTGAGATGAAGATGA+AGG + chr8_4:54453984-54454003 None:intergenic 35.0%
ACTATCTTCCATTTCCATTG+TGG - chr8_4:54454069-54454088 Msa1334140:three_prime_UTR 35.0%
GAAGATGAAAACACTAACGT+TGG + chr8_4:54454130-54454149 None:intergenic 35.0%
! TTTTCATCTTCTTCCACTTC+AGG - chr8_4:54454139-54454158 Msa1334140:three_prime_UTR 35.0%
AATTTCTTGAAGCCAGAATG+CGG + chr8_4:54454211-54454230 None:intergenic 35.0%
! ATAAGGTTTTCACTTAGCTG+AGG + chr8_4:54454358-54454377 None:intergenic 35.0%
CTATCTTCACACCATAATAC+AGG + chr8_4:54454541-54454560 None:intergenic 35.0%
AAATACAATCCGTGTTCACA+GGG + chr8_4:54454651-54454670 None:intergenic 35.0%
TAAATACAATCCGTGTTCAC+AGG + chr8_4:54454652-54454671 None:intergenic 35.0%
ATCTTGTTGAAGTGCAGTAT+AGG - chr8_4:54454677-54454696 Msa1334140:intron 35.0%
ATTTAACCACAGATGAAGGA+TGG - chr8_4:54454725-54454744 Msa1334140:intron 35.0%
TTTAACCACAGATGAAGGAT+GGG - chr8_4:54454726-54454745 Msa1334140:intron 35.0%
!! AAGATGTAACTGGCTTGTTA+TGG - chr8_4:54454777-54454796 Msa1334140:intron 35.0%
GGCTAGTGATAAGATTATCA+CGG - chr8_4:54454909-54454928 Msa1334140:intron 35.0%
GCTAGTGATAAGATTATCAC+GGG - chr8_4:54454910-54454929 Msa1334140:intron 35.0%
!! ATCGGTGTTATTAGAGTTTG+TGG - chr8_4:54455024-54455043 Msa1334140:intron 35.0%
ATGAATTGCACCTTCATTGT+GGG + chr8_4:54455052-54455071 None:intergenic 35.0%
GATTTGATTGAGATTATCCG+TGG - chr8_4:54455072-54455091 Msa1334140:intron 35.0%
TTTGATTGAGATTATCCGTG+GGG - chr8_4:54455074-54455093 Msa1334140:intron 35.0%
TGGGTCATCAAAAAGTTTGA+AGG - chr8_4:54455098-54455117 Msa1334140:intron 35.0%
! TCTGATAATGAAGAAACGAC+TGG + chr8_4:54455213-54455232 None:intergenic 35.0%
CGTTTCTTCATTATCAGACA+AGG - chr8_4:54455215-54455234 Msa1334140:intron 35.0%
AAGATGAGCGGATCATAAAT+GGG - chr8_4:54455445-54455464 Msa1334140:intron 35.0%
TGTTGCTATAGCCAAATTGA+AGG - chr8_4:54455623-54455642 Msa1334140:intron 35.0%
GAGAGTTCAACCAGATTAAT+AGG + chr8_4:54455669-54455688 None:intergenic 35.0%
! CGTAATTTGAGGTTTAAACG+AGG - chr8_4:54455691-54455710 Msa1334140:intron 35.0%
!! CTTTTACTGGTTGAACTCTT+TGG - chr8_4:54455735-54455754 Msa1334140:intron 35.0%
!! TGGAAAGTGGACTTTTACAT+TGG - chr8_4:54455879-54455898 Msa1334140:intron 35.0%
TTGGATAGTTTGGATAGTCA+TGG - chr8_4:54455898-54455917 Msa1334140:intron 35.0%
!!! GTTTGTTTTTTCCCCTTTTC+TGG - chr8_4:54456040-54456059 Msa1334140:intron 35.0%
!! GTGATCAAATTTTAGTCCAG+CGG + chr8_4:54456075-54456094 None:intergenic 35.0%
AGTAACAAGTGCTTAGTTGT+TGG + chr8_4:54456121-54456140 None:intergenic 35.0%
TCTTTGCTTTGCAATATTGG+TGG - chr8_4:54456168-54456187 Msa1334140:intron 35.0%
! GCAATATTGGTGGTATCTAA+TGG - chr8_4:54456178-54456197 Msa1334140:intron 35.0%
AATATTCTTCCTTCAAGGAC+AGG - chr8_4:54456210-54456229 Msa1334140:intron 35.0%
! TCTGATAGTGCAGTGTTATT+TGG - chr8_4:54456558-54456577 Msa1334140:CDS 35.0%
TATGTACGTGAAGAATCGTT+TGG + chr8_4:54456618-54456637 None:intergenic 35.0%
! CACTGACTGATTAACTTGAT+AGG - chr8_4:54456646-54456665 Msa1334140:CDS 35.0%
CATGGATAGTTAGGAATAGA+GGG + chr8_4:54456909-54456928 None:intergenic 35.0%
TCATGGATAGTTAGGAATAG+AGG + chr8_4:54456910-54456929 None:intergenic 35.0%
CTATTCCTAACTATCCATGA+AGG - chr8_4:54456910-54456929 Msa1334140:CDS 35.0%
GGAATGGCATTAGTAGTTTA+CGG + chr8_4:54457135-54457154 None:intergenic 35.0%
GGAATGGCATTAGTAGTTTA+CGG + chr8_4:54457135-54457154 None:intergenic 35.0%
! GTTTTCTTTTTTCTGCAGTC+TGG - chr8_4:54457504-54457523 Msa1334140:CDS 35.0%
GTAAAACAACAGTCCTTTGA+AGG + chr8_4:54457540-54457559 None:intergenic 35.0%
CTTATCAGAACACAACAAAC+TGG - chr8_4:54457559-54457578 Msa1334140:CDS 35.0%
TTGGTATAAGTACCCATTTC+GGG + chr8_4:54457591-54457610 None:intergenic 35.0%
GTTGGTATAAGTACCCATTT+CGG + chr8_4:54457592-54457611 None:intergenic 35.0%
TGTAGATGATTTAGGCCATT+TGG - chr8_4:54457735-54457754 Msa1334140:CDS 35.0%
CTCAAAACAGTATCAAATCC+AGG + chr8_4:54457797-54457816 None:intergenic 35.0%
! AAGAGCTTGCTTTAACAGAA+GGG + chr8_4:54457921-54457940 None:intergenic 35.0%
! GTTGACATTTTACCACTGTT+AGG - chr8_4:54457957-54457976 Msa1334140:CDS 35.0%
TTCAAAAGTTGACAGAGTGA+AGG + chr8_4:54458107-54458126 None:intergenic 35.0%
TTTGTGAATGTGAGGGGTTT+CGG + chr8_4:54454029-54454048 None:intergenic 40.0%
TGAGGGATTTGTGAATGTGA+GGG + chr8_4:54454036-54454055 None:intergenic 40.0%
TTGAGGGATTTGTGAATGTG+AGG + chr8_4:54454037-54454056 None:intergenic 40.0%
TAGTGTGCGGTAGAATTTGA+GGG + chr8_4:54454053-54454072 None:intergenic 40.0%
ATAGTGTGCGGTAGAATTTG+AGG + chr8_4:54454054-54454073 None:intergenic 40.0%
TGGAAATGGAAGATAGTGTG+CGG + chr8_4:54454066-54454085 None:intergenic 40.0%
GAAGTAAAGATGCTGCAACA+CGG + chr8_4:54454096-54454115 None:intergenic 40.0%
!! ACTAACGTTGGTTCTTGAGT+AGG + chr8_4:54454118-54454137 None:intergenic 40.0%
TCTTCTTCCACTTCAGGTAA+TGG - chr8_4:54454145-54454164 Msa1334140:three_prime_UTR 40.0%
! TCCACTTCAGGTAATGGTAA+CGG - chr8_4:54454151-54454170 Msa1334140:three_prime_UTR 40.0%
!!! TAACGGTGTTGATTTTCCCA+AGG - chr8_4:54454168-54454187 Msa1334140:three_prime_UTR 40.0%
!!! AACGGTGTTGATTTTCCCAA+GGG - chr8_4:54454169-54454188 Msa1334140:three_prime_UTR 40.0%
ACGAAATCAAACTCACCCTT+GGG + chr8_4:54454187-54454206 None:intergenic 40.0%
ATTTCTTGAAGCCAGAATGC+GGG + chr8_4:54454210-54454229 None:intergenic 40.0%
AATATTTCCGATGGACCAGT+TGG - chr8_4:54454307-54454326 Msa1334140:three_prime_UTR 40.0%
GATCAAATGCGGCTTTCATA+AGG + chr8_4:54454375-54454394 None:intergenic 40.0%
!!! GGAGTTTTGAGCCTGTATTA+TGG - chr8_4:54454527-54454546 Msa1334140:CDS 40.0%
CTTGTATCTGGAGAATCATC+CGG + chr8_4:54454619-54454638 None:intergenic 40.0%
GGGCTATCAATACTTGTATC+TGG + chr8_4:54454631-54454650 None:intergenic 40.0%
! TATTGATAGCCCTGTGAACA+CGG - chr8_4:54454639-54454658 Msa1334140:CDS 40.0%
GGACATTTAACCACAGATGA+AGG - chr8_4:54454721-54454740 Msa1334140:intron 40.0%
! TAGGTTACTTTCAGGGTTGA+TGG - chr8_4:54454888-54454907 Msa1334140:intron 40.0%
!! TATGCTTTGAAATCGAGCAG+TGG + chr8_4:54454955-54454974 None:intergenic 40.0%
TGCACTGTTGGTATGTCAAA+TGG - chr8_4:54454991-54455010 Msa1334140:intron 40.0%
TGTTGGTATGTCAAATGGCA+GGG - chr8_4:54454996-54455015 Msa1334140:intron 40.0%
TCAAATGGCAGGGAAAAGAT+CGG - chr8_4:54455006-54455025 Msa1334140:intron 40.0%
CATGAATTGCACCTTCATTG+TGG + chr8_4:54455053-54455072 None:intergenic 40.0%
! TCCAGGAGGAGAATTTTTGT+TGG - chr8_4:54455143-54455162 Msa1334140:intron 40.0%
TTATCAGACAAGGCAACTAG+TGG - chr8_4:54455225-54455244 Msa1334140:intron 40.0%
GGTGCATATTTGATTGCAAC+TGG - chr8_4:54455246-54455265 Msa1334140:intron 40.0%
TATTTGATTGCAACTGGAGC+AGG - chr8_4:54455252-54455271 Msa1334140:intron 40.0%
TCATCTTCATGTGGCATGAT+TGG + chr8_4:54455432-54455451 None:intergenic 40.0%
GAAGATGAGCGGATCATAAA+TGG - chr8_4:54455444-54455463 Msa1334140:intron 40.0%
TTACAAAGTCTTTGTGGGCA+AGG - chr8_4:54455479-54455498 Msa1334140:intron 40.0%
CACATCTACACTTAACGATC+TGG + chr8_4:54455514-54455533 None:intergenic 40.0%
CAGATCGTTAAGTGTAGATG+TGG - chr8_4:54455512-54455531 Msa1334140:intron 40.0%
TGAACTCTCTGCGTAATTTG+AGG - chr8_4:54455680-54455699 Msa1334140:intron 40.0%
TGGTTTCTTGCTGAAAAGTG+TGG - chr8_4:54455918-54455937 Msa1334140:intron 40.0%
AAGTGCTTAGTTGTTGGCAA+AGG + chr8_4:54456115-54456134 None:intergenic 40.0%
TGTTAATGTCCTGTCCTTGA+AGG + chr8_4:54456222-54456241 None:intergenic 40.0%
ATGCAAACAAGAGCTTGGAA+AGG + chr8_4:54456404-54456423 None:intergenic 40.0%
! GCATTTATTTGGCTCACACT+TGG - chr8_4:54456420-54456439 Msa1334140:intron 40.0%
GATGCTCTCATATTCCATGT+AGG - chr8_4:54456852-54456871 Msa1334140:CDS 40.0%
GGTGATATTGTCAACCTACA+TGG + chr8_4:54456869-54456888 None:intergenic 40.0%
GTCAACCTTCATGGATAGTT+AGG + chr8_4:54456918-54456937 None:intergenic 40.0%
TGGATGTTGTATGCCTTCAA+AGG - chr8_4:54457524-54457543 Msa1334140:CDS 40.0%
GTACTTATACCAACCCTGAT+TGG - chr8_4:54457598-54457617 Msa1334140:CDS 40.0%
TCAACATCAGTGTACTCCAT+TGG - chr8_4:54457636-54457655 Msa1334140:CDS 40.0%
GATGTGCATTCAGGAATAAC+AGG - chr8_4:54457661-54457680 Msa1334140:CDS 40.0%
TGCATTCAGGAATAACAGGA+GGG - chr8_4:54457665-54457684 Msa1334140:CDS 40.0%
! TAGTGTGGGTTTTTATGTCC+TGG - chr8_4:54457776-54457795 Msa1334140:CDS 40.0%
! GCTTGACTCTGCTGTTTAAA+TGG - chr8_4:54457821-54457840 Msa1334140:CDS 40.0%
! ATGACTTAGTTTGGCCAAAC+TGG - chr8_4:54457892-54457911 Msa1334140:CDS 40.0%
! CAAGAGCTTGCTTTAACAGA+AGG + chr8_4:54457922-54457941 None:intergenic 40.0%
CTTCTGTTAAAGCAAGCTCT+TGG - chr8_4:54457920-54457939 Msa1334140:CDS 40.0%
! GTGCTAAGAGCTTGCTTTAA+GGG + chr8_4:54457986-54458005 None:intergenic 40.0%
! TGTGCTAAGAGCTTGCTTTA+AGG + chr8_4:54457987-54458006 None:intergenic 40.0%
!! CTGGTATGATTTGTTTGTCC+TGG - chr8_4:54458187-54458206 Msa1334140:CDS 40.0%
TTTCGGGAAGATGAAGAGGA+TGG + chr8_4:54454012-54454031 None:intergenic 45.0%
TTGTGAATGTGAGGGGTTTC+GGG + chr8_4:54454028-54454047 None:intergenic 45.0%
GAGGGATTTGTGAATGTGAG+GGG + chr8_4:54454035-54454054 None:intergenic 45.0%
AACACGGTCCACAATGGAAA+TGG + chr8_4:54454080-54454099 None:intergenic 45.0%
ACCGTTACCATTACCTGAAG+TGG + chr8_4:54454155-54454174 None:intergenic 45.0%
GACGAAATCAAACTCACCCT+TGG + chr8_4:54454188-54454207 None:intergenic 45.0%
! TTTGATTTCGTCCCGCATTC+TGG - chr8_4:54454196-54454215 Msa1334140:three_prime_UTR 45.0%
! GGTTAAGACTCTGTTAGCCT+CGG - chr8_4:54454240-54454259 Msa1334140:three_prime_UTR 45.0%
TATCTGGAGAATCATCCGGA+GGG + chr8_4:54454615-54454634 None:intergenic 45.0%
TGAAGTGCAGTATAGGACAG+CGG - chr8_4:54454684-54454703 Msa1334140:intron 45.0%
ACAGCGGGGAAATACAAATC+TGG - chr8_4:54454700-54454719 Msa1334140:intron 45.0%
TGAAGGATGGGAATCGATGA+AGG - chr8_4:54454738-54454757 Msa1334140:intron 45.0%
GGAATCGATGAAGGCAGTTA+AGG - chr8_4:54454747-54454766 Msa1334140:intron 45.0%
AGATTATCACGGGTCTCAGT+AGG - chr8_4:54454920-54454939 Msa1334140:intron 45.0%
CTGTTGGTATGTCAAATGGC+AGG - chr8_4:54454995-54455014 Msa1334140:intron 45.0%
GTTTGTGGATCCCACAATGA+AGG - chr8_4:54455039-54455058 Msa1334140:intron 45.0%
GTTTGAAGGCTGTAATCCTC+CGG - chr8_4:54455112-54455131 Msa1334140:intron 45.0%
GCCAACAAAAATTCTCCTCC+TGG + chr8_4:54455147-54455166 None:intergenic 45.0%
AAGAAGGAGGTTCTTCACAG+AGG - chr8_4:54455369-54455388 Msa1334140:intron 45.0%
TCATGCCACATGAAGATGAG+CGG - chr8_4:54455433-54455452 Msa1334140:intron 45.0%
ATGATCCGCTCATCTTCATG+TGG + chr8_4:54455441-54455460 None:intergenic 45.0%
CAGCACGTAGCTGATATATC+TGG - chr8_4:54456486-54456505 Msa1334140:intron 45.0%
GTTAATCAGTCAGTGCACTC+AGG + chr8_4:54456641-54456660 None:intergenic 45.0%
!! GATGTATTTCTGTCGGCTTC+CGG - chr8_4:54457442-54457461 Msa1334140:CDS 45.0%
AAACTGGACAAAGCCCGAAA+TGG - chr8_4:54457575-54457594 Msa1334140:CDS 45.0%
AACTGGACAAAGCCCGAAAT+GGG - chr8_4:54457576-54457595 Msa1334140:CDS 45.0%
TACCAACCCTGATTGGAAGA+AGG - chr8_4:54457605-54457624 Msa1334140:CDS 45.0%
ACAGCTCCTTCTTCCAATCA+GGG + chr8_4:54457614-54457633 None:intergenic 45.0%
AACAGCTCCTTCTTCCAATC+AGG + chr8_4:54457615-54457634 None:intergenic 45.0%
CCTGAATGCACATCAACCAA+TGG + chr8_4:54457655-54457674 None:intergenic 45.0%
! CCATTGGTTGATGTGCATTC+AGG - chr8_4:54457652-54457671 Msa1334140:CDS 45.0%
GTGCATTCAGGAATAACAGG+AGG - chr8_4:54457664-54457683 Msa1334140:CDS 45.0%
TTCAGGAATAACAGGAGGGA+TGG - chr8_4:54457669-54457688 Msa1334140:CDS 45.0%
TAACAGAAGGGAAGCCAGTT+TGG + chr8_4:54457909-54457928 None:intergenic 45.0%
! CTTTAAGGGAAGCCTAACAG+TGG + chr8_4:54457972-54457991 None:intergenic 45.0%
!!! TCTTATAAATTTAATTTAAT+CGG + chr8_4:54455816-54455835 None:intergenic 5.0%
!! ACCAGTTGGCACTGACTCTA+AGG - chr8_4:54454321-54454340 Msa1334140:three_prime_UTR 50.0%
ACCTTAGAGTCAGTGCCAAC+TGG + chr8_4:54454325-54454344 None:intergenic 50.0%
GGACACACGAGGATCAAATG+CGG + chr8_4:54454386-54454405 None:intergenic 50.0%
ATCTGGAGAATCATCCGGAG+GGG + chr8_4:54454614-54454633 None:intergenic 50.0%
GTATCTGGAGAATCATCCGG+AGG + chr8_4:54454616-54454635 None:intergenic 50.0%
GAAGTGCAGTATAGGACAGC+GGG - chr8_4:54454685-54454704 Msa1334140:intron 50.0%
AAGTGCAGTATAGGACAGCG+GGG - chr8_4:54454686-54454705 Msa1334140:intron 50.0%
CGATTCCCATCCTTCATCTG+TGG + chr8_4:54454734-54454753 None:intergenic 50.0%
ACGGGTCTCAGTAGGAGATT+TGG - chr8_4:54454928-54454947 Msa1334140:intron 50.0%
ATTGAGATTATCCGTGGGGC+TGG - chr8_4:54455078-54455097 Msa1334140:intron 50.0%
TTGAGATTATCCGTGGGGCT+GGG - chr8_4:54455079-54455098 Msa1334140:intron 50.0%
!! TTTTTGATGACCCAGCCCCA+CGG + chr8_4:54455092-54455111 None:intergenic 50.0%
AAGGAGGTTCTTCACAGAGG+TGG - chr8_4:54455372-54455391 Msa1334140:intron 50.0%
TGGTTGACGAGATTGCCCAA+GGG - chr8_4:54455532-54455551 Msa1334140:intron 50.0%
CAAGCGCGTCAACCAACATA+TGG + chr8_4:54455587-54455606 None:intergenic 50.0%
! CATATGTTGGTTGACGCGCT+TGG - chr8_4:54455585-54455604 Msa1334140:intron 50.0%
GGCACTAGCATCCAGAAAAG+GGG + chr8_4:54456054-54456073 None:intergenic 50.0%
CGGCACTAGCATCCAGAAAA+GGG + chr8_4:54456055-54456074 None:intergenic 50.0%
ACTTCAGTCCCCTGTGCTTA+AGG - chr8_4:54457050-54457069 Msa1334140:CDS 50.0%
AACCTCTGCCTTAAGCACAG+GGG + chr8_4:54457061-54457080 None:intergenic 50.0%
GAACCTCTGCCTTAAGCACA+GGG + chr8_4:54457062-54457081 None:intergenic 50.0%
AGAACCTCTGCCTTAAGCAC+AGG + chr8_4:54457063-54457082 None:intergenic 50.0%
ACTTCAGTCCCCTGTGCTTA+AGG - chr8_4:54457050-54457069 Msa1334140:CDS 50.0%
AACCTCTGCCTTAAGCACAG+GGG + chr8_4:54457061-54457080 None:intergenic 50.0%
GAACCTCTGCCTTAAGCACA+GGG + chr8_4:54457062-54457081 None:intergenic 50.0%
AGAACCTCTGCCTTAAGCAC+AGG + chr8_4:54457063-54457082 None:intergenic 50.0%
CTCCTTCTTCCAATCAGGGT+TGG + chr8_4:54457610-54457629 None:intergenic 50.0%
ACAGGTTCACAGATAGCTCC+AGG + chr8_4:54458208-54458227 None:intergenic 50.0%
GGGGTTTCGGGAAGATGAAG+AGG + chr8_4:54454016-54454035 None:intergenic 55.0%
TGCTGCAACACGGTCCACAA+TGG + chr8_4:54454086-54454105 None:intergenic 55.0%
! TCTGTTAGCCTCGGAGCATG+TGG - chr8_4:54454249-54454268 Msa1334140:three_prime_UTR 55.0%
TCCATGTTCCACATGCTCCG+AGG + chr8_4:54454260-54454279 None:intergenic 55.0%
GTCAGTGCCAACTGGTCCAT+CGG + chr8_4:54454317-54454336 None:intergenic 55.0%
AGAAACACAGCGGACACACG+AGG + chr8_4:54454397-54454416 None:intergenic 55.0%
! GAATCATCCGGAGGGGGTTT+TGG + chr8_4:54454607-54454626 None:intergenic 55.0%
TCTGGAGAATCATCCGGAGG+GGG + chr8_4:54454613-54454632 None:intergenic 55.0%
ATCCTCCGGATTGACAGTCC+AGG - chr8_4:54455126-54455145 Msa1334140:intron 55.0%
CTCCTCCTGGACTGTCAATC+CGG + chr8_4:54455134-54455153 None:intergenic 55.0%
CTTTGTGGGCAAGGTAGCGA+TGG - chr8_4:54455488-54455507 Msa1334140:intron 55.0%
GTGGTTGACGAGATTGCCCA+AGG - chr8_4:54455531-54455550 Msa1334140:intron 55.0%
TTGACGAGATTGCCCAAGGG+CGG - chr8_4:54455535-54455554 Msa1334140:intron 55.0%
! GGCGGCACTGAACCATATGT+TGG - chr8_4:54455572-54455591 Msa1334140:intron 55.0%
! ATGTTGGTTGACGCGCTTGG+TGG - chr8_4:54455588-54455607 Msa1334140:intron 55.0%
GCGGCACTAGCATCCAGAAA+AGG + chr8_4:54456056-54456075 None:intergenic 55.0%
TTTCTGGATGCTAGTGCCGC+TGG - chr8_4:54456056-54456075 Msa1334140:intron 55.0%
!! TTCTGTCGGCTTCCGGTTTC+TGG - chr8_4:54457449-54457468 Msa1334140:CDS 55.0%
GCCTCGGAGCATGTGGAACA+TGG - chr8_4:54454256-54454275 Msa1334140:three_prime_UTR 60.0%
TGCACCTCCAAAACCCCCTC+CGG - chr8_4:54454597-54454616 Msa1334140:CDS 60.0%
!! TCATCCGGAGGGGGTTTTGG+AGG + chr8_4:54454604-54454623 None:intergenic 60.0%
CTCCTGGACTGTCAATCCGG+AGG + chr8_4:54455131-54455150 None:intergenic 60.0%
CTCCGGATTGACAGTCCAGG+AGG - chr8_4:54455129-54455148 Msa1334140:intron 60.0%
AGATTGCCCAAGGGCGGCTT+TGG - chr8_4:54455541-54455560 Msa1334140:intron 60.0%
ATGGTTCAGTGCCGCCTTCC+CGG + chr8_4:54455568-54455587 None:intergenic 60.0%
GTCCCCTGTGCTTAAGGCAG+AGG - chr8_4:54457056-54457075 Msa1334140:CDS 60.0%
GTCCCCTGTGCTTAAGGCAG+AGG - chr8_4:54457056-54457075 Msa1334140:CDS 60.0%
!! GCCCAAGGGCGGCTTTGGAC+CGG - chr8_4:54455546-54455565 Msa1334140:intron 70.0%
CCCGGTCCAAAGCCGCCCTT+GGG + chr8_4:54455550-54455569 None:intergenic 70.0%
!! CCCAAGGGCGGCTTTGGACC+GGG - chr8_4:54455547-54455566 Msa1334140:intron 70.0%
TCCCGGTCCAAAGCCGCCCT+TGG + chr8_4:54455551-54455570 None:intergenic 70.0%
!! AGGGCGGCTTTGGACCGGGA+AGG - chr8_4:54455551-54455570 Msa1334140:intron 70.0%
!! GCGGCTTTGGACCGGGAAGG+CGG - chr8_4:54455554-54455573 Msa1334140:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 54453944 54458249 54453944 ID=Msa1334140;Name=Msa1334140
chr8_4 mRNA 54453944 54458249 54453944 ID=Msa1334140-mRNA-1;Parent=Msa1334140;Name=Msa1334140-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0.5|1|1|1|2|538|645
chr8_4 exon 54453944 54454671 54453944 ID=Msa1334140-mRNA-1:exon:16934;Parent=Msa1334140-mRNA-1
chr8_4 exon 54456502 54458249 54456502 ID=Msa1334140-mRNA-1:exon:16933;Parent=Msa1334140-mRNA-1
chr8_4 CDS 54456502 54458249 54456502 ID=Msa1334140-mRNA-1:cds;Parent=Msa1334140-mRNA-1
chr8_4 CDS 54454482 54454671 54454482 ID=Msa1334140-mRNA-1:cds;Parent=Msa1334140-mRNA-1
chr8_4 three_prime_UTR 54453944 54454481 54453944 ID=Msa1334140-mRNA-1:three_prime_utr;Parent=Msa1334140-mRNA-1
Gene Sequence

>Msa1334140

ATGCTTCGTTACATGTACAGAAAAGCATCACAATCATCCTTCATCTTCATCTCAAACAACTTACGCCATCCTCTTCATCTTCCCGAAACCCCTCACATTCACAAATCCCTCAAATTCTACCGCACACTATCTTCCATTTCCATTGTGGACCGTGTTGCAGCATCTTTACTTCCTACTCAAGAACCAACGTTAGTGTTTTCATCTTCTTCCACTTCAGGTAATGGTAACGGTGTTGATTTTCCCAAGGGTGAGTTTGATTTCGTCCCGCATTCTGGCTTCAAGAAATTTATTGTGAAGGTTAAGACTCTGTTAGCCTCGGAGCATGTGGAACATGGAAGTATATTGAGAATCAATTTACAAGGAAATATTTCCGATGGACCAGTTGGCACTGACTCTAAGGTATTGTCTTTACCTCAGCTAAGTGAAAACCTTATGAAAGCCGCATTTGATCCTCGTGTGTCCGCTGTGTTTCTTAATATAGTTGATTTTTATTGTGGTTGGGCTAAATGTGATGAAATTAGAAGACAGATATCAAATTTTAAAAAATCTGGTAAAAAGGTTATAGCTTTTATGGCTTCATTTTGGAGTTTTGAGCCTGTATTATGGTGTGAAGATAGATCATATTACATAGCTAGTGTATGTGATGAAATATATGCACCTCCAAAACCCCCTCCGGATGATTCTCCAGATACAAGTATTGATAGCCCTGTGAACACGGATTGTATTTATTACAATCTTGTTGAAGTGCAGTATAGGACAGCGGGGAAATACAAATCTGGACATTTAACCACAGATGAAGGATGGGAATCGATGAAGGCAGTTAAGGATGATATAAGATGTAACTGGCTTGTTATGGTTTCTTCTTCAAGGAAAATAAGAGAAGAGGAGATTCTAACTTTTTTTGGTAGTGAGGATTGTCGTGAAGTAGAAAATTGGATAGATTGTAGGTTACTTTCAGGGTTGATGGCTAGTGATAAGATTATCACGGGTCTCAGTAGGAGATTTGGACCACTGCTCGATTTCAAAGCATATTCACAAGTTAAAAAATGCACTGTTGGTATGTCAAATGGCAGGGAAAAGATCGGTGTTATTAGAGTTTGTGGATCCCACAATGAAGGTGCAATTCATGATTTGATTGAGATTATCCGTGGGGCTGGGTCATCAAAAAGTTTGAAGGCTGTAATCCTCCGGATTGACAGTCCAGGAGGAGAATTTTTGTTGGCTGATCAATTATGGAGAGAAGTCAACATTCTTGCTTCAAAGATACCAGTCGTTTCTTCATTATCAGACAAGGCAACTAGTGGTGCATATTTGATTGCAACTGGAGCAGGAACAATTGTTGCTGAGAAACTCACTTATACATGTCAAATTGGAGCGATAAACTCATTTCCAAACACTGAGAAGTTATACAAATACATTGGTTTTAAGAAGGAGGTTCTTCACAGAGGTGGTAAATATGCAGAATTATTTGAGGCTGATCATCGGCCAATCATGCCACATGAAGATGAGCGGATCATAAATGGGTTAGAAGAAGTTTACAAAGTCTTTGTGGGCAAGGTAGCGATGGCCAGATCGTTAAGTGTAGATGTGGTTGACGAGATTGCCCAAGGGCGGCTTTGGACCGGGAAGGCGGCACTGAACCATATGTTGGTTGACGCGCTTGGTGGTTTTTCACGCGCTGTTGCTATAGCCAAATTGAAGGCTAATATACCTCAAAATAGACCTATTAATCTGGTTGAACTCTCTGCTCTGGATGTTGTATGCCTTCAAAGGACTGTTGTTTTACTTATCAGAACACAACAAACTGGACAAAGCCCGAAATGGGTACTTATACCAACCCTGATTGGAAGAAGGAGCTGTTGTCAACATCAGTGTACTCCATTGGTTGATGTGCATTCAGGAATAACAGGAGGGATGGTCTCTTCAATTCAACAGTGA
Protein sequence

>Msa1334140

MLRYMYRKASQSSFIFISNNLRHPLHLPETPHIHKSLKFYRTLSSISIVDRVAASLLPTQEPTLVFSSSSTSGNGNGVDFPKGEFDFVPHSGFKKFIVKVKTLLASEHVEHGSILRINLQGNISDGPVGTDSKVLSLPQLSENLMKAAFDPRVSAVFLNIVDFYCGWAKCDEIRRQISNFKKSGKKVIAFMASFWSFEPVLWCEDRSYYIASVCDEIYAPPKPPPDDSPDTSIDSPVNTDCIYYNLVEVQYRTAGKYKSGHLTTDEGWESMKAVKDDIRCNWLVMVSSSRKIREEEILTFFGSEDCREVENWIDCRLLSGLMASDKIITGLSRRFGPLLDFKAYSQVKKCTVGMSNGREKIGVIRVCGSHNEGAIHDLIEIIRGAGSSKSLKAVILRIDSPGGEFLLADQLWREVNILASKIPVVSSLSDKATSGAYLIATGAGTIVAEKLTYTCQIGAINSFPNTEKLYKYIGFKKEVLHRGGKYAELFEADHRPIMPHEDERIINGLEEVYKVFVGKVAMARSLSVDVVDEIAQGRLWTGKAALNHMLVDALGGFSRAVAIAKLKANIPQNRPINLVELSALDVVCLQRTVVLLIRTQQTGQSPKWVLIPTLIGRRSCCQHQCTPLVDVHSGITGGMVSSIQQ*