Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1355490 | XP_024637122.1 | 96.552 | 58 | 2 | 0 | 1 | 58 | 74 | 131 | 2.49e-29 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1355490 | sp|Q9FZ89|Y1815_ARATH | 70.000 | 60 | 16 | 1 | 1 | 58 | 64 | 123 | 8.08e-22 | 84.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1355490 | A0A072VXV9 | 96.552 | 58 | 2 | 0 | 1 | 58 | 73 | 130 | 1.22e-29 | 110 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0030960 | Msa1355490 | 0.859046 | 5.421099e-63 | -8.615850e-47 |
| Msa0042210 | Msa1355490 | 0.802983 | 4.329769e-49 | -8.615850e-47 |
| Msa0780780 | Msa1355490 | 0.806335 | 8.649384e-50 | -8.615850e-47 |
| Msa0788040 | Msa1355490 | 0.801926 | 7.148484e-49 | -8.615850e-47 |
| Msa0126000 | Msa1355490 | 0.812323 | 4.491300e-51 | -8.615850e-47 |
| Msa0168470 | Msa1355490 | 0.813180 | 2.916733e-51 | -8.615850e-47 |
| Msa1244770 | Msa1355490 | 0.810801 | 9.623404e-51 | -8.615850e-47 |
| Msa1317380 | Msa1355490 | 0.818114 | 2.320456e-52 | -8.615850e-47 |
| Msa0441840 | Msa1355490 | 0.806409 | 8.342985e-50 | -8.615850e-47 |
| Msa0450390 | Msa1355490 | 0.817687 | 2.897374e-52 | -8.615850e-47 |
| Msa1066820 | Msa1355490 | 0.812301 | 4.542779e-51 | -8.615850e-47 |
| Msa1066950 | Msa1355490 | 0.858276 | 9.207423e-63 | -8.615850e-47 |
| Msa1082640 | Msa1355490 | 0.827330 | 1.660814e-54 | -8.615850e-47 |
| Msa0293000 | Msa1355490 | 0.820822 | 5.598683e-53 | -8.615850e-47 |
| Msa0331060 | Msa1355490 | 0.814267 | 1.680887e-51 | -8.615850e-47 |
| Msa0489460 | Msa1355490 | 0.806808 | 6.871413e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1355490 | MtrunA17_Chr1g0188791 | 96.552 | 58 | 2 | 0 | 1 | 58 | 73 | 130 | 2.35e-33 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1355490 | AT1G28150.1 | 70.000 | 60 | 16 | 1 | 1 | 58 | 64 | 123 | 8.21e-23 | 84.0 |
| Msa1355490 | AT1G28150.2 | 70.000 | 60 | 16 | 1 | 1 | 58 | 64 | 123 | 8.21e-23 | 84.0 |
Find 15 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTCAGTAGTTGATTCTTC+TGG | 0.258189 | tig0014794:+55723 | None:intergenic |
| TTCTTAGGTGGGATATTTGC+TGG | 0.305119 | tig0014794:-55837 | Msa1355490:CDS |
| CAGGAACCAGTGGCATTCTT+AGG | 0.325742 | tig0014794:-55852 | Msa1355490:CDS |
| AGTAGTTGATTCTTCTGGAT+TGG | 0.410215 | tig0014794:+55728 | None:intergenic |
| TATTCAATTTCAATCTCTTG+TGG | 0.483856 | tig0014794:+55696 | None:intergenic |
| ATGAGGAACCTCTGAAGGAA+TGG | 0.505614 | tig0014794:-55794 | Msa1355490:CDS |
| TTTAAGGCTTGATTTGAATG+AGG | 0.510298 | tig0014794:-55811 | Msa1355490:CDS |
| TGAGGAACCTCTGAAGGAAT+GGG | 0.523835 | tig0014794:-55793 | Msa1355490:CDS |
| CTCGTAACCCATTCCTTCAG+AGG | 0.539677 | tig0014794:+55786 | None:intergenic |
| TTCTAATCTGCAGGAACCAG+TGG | 0.542541 | tig0014794:-55862 | None:intergenic |
| GAACCAGTGGCATTCTTAGG+TGG | 0.545711 | tig0014794:-55849 | Msa1355490:CDS |
| TTTGAATGAGGAACCTCTGA+AGG | 0.563194 | tig0014794:-55799 | Msa1355490:CDS |
| ATCCCACCTAAGAATGCCAC+TGG | 0.574031 | tig0014794:+55846 | None:intergenic |
| CTCTGAAGGAATGGGTTACG+AGG | 0.596910 | tig0014794:-55785 | Msa1355490:CDS |
| AACCAGTGGCATTCTTAGGT+GGG | 0.598806 | tig0014794:-55848 | Msa1355490:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATTCAATTTCAATCTCTTG+TGG | + | tig0014794:55851-55870 | None:intergenic | 25.0% |
| ! | TATTCAATTTCAATCTCTTG+TGG | + | tig0014794:55851-55870 | None:intergenic | 25.0% |
| !! | GGATATTTGCTGGTATTTTA+AGG | - | tig0014794:55717-55736 | Msa1355490:CDS | 30.0% |
| ! | TTTAAGGCTTGATTTGAATG+AGG | - | tig0014794:55733-55752 | Msa1355490:CDS | 30.0% |
| !! | GGATATTTGCTGGTATTTTA+AGG | - | tig0014794:55717-55736 | Msa1355490:CDS | 30.0% |
| ! | TTTAAGGCTTGATTTGAATG+AGG | - | tig0014794:55733-55752 | Msa1355490:CDS | 30.0% |
| ! | AGTAGTTGATTCTTCTGGAT+TGG | + | tig0014794:55819-55838 | None:intergenic | 35.0% |
| ! | AGTAGTTGATTCTTCTGGAT+TGG | + | tig0014794:55819-55838 | None:intergenic | 35.0% |
| TTCTTAGGTGGGATATTTGC+TGG | - | tig0014794:55707-55726 | Msa1355490:CDS | 40.0% | |
| TTTGAATGAGGAACCTCTGA+AGG | - | tig0014794:55745-55764 | Msa1355490:CDS | 40.0% | |
| ! | GCTTCAGTAGTTGATTCTTC+TGG | + | tig0014794:55824-55843 | None:intergenic | 40.0% |
| TTCTTAGGTGGGATATTTGC+TGG | - | tig0014794:55707-55726 | Msa1355490:CDS | 40.0% | |
| TTTGAATGAGGAACCTCTGA+AGG | - | tig0014794:55745-55764 | Msa1355490:CDS | 40.0% | |
| ! | GCTTCAGTAGTTGATTCTTC+TGG | + | tig0014794:55824-55843 | None:intergenic | 40.0% |
| AACCAGTGGCATTCTTAGGT+GGG | - | tig0014794:55696-55715 | Msa1355490:CDS | 45.0% | |
| ! | ATGAGGAACCTCTGAAGGAA+TGG | - | tig0014794:55750-55769 | Msa1355490:CDS | 45.0% |
| ! | TGAGGAACCTCTGAAGGAAT+GGG | - | tig0014794:55751-55770 | Msa1355490:CDS | 45.0% |
| AACCAGTGGCATTCTTAGGT+GGG | - | tig0014794:55696-55715 | Msa1355490:CDS | 45.0% | |
| ! | ATGAGGAACCTCTGAAGGAA+TGG | - | tig0014794:55750-55769 | Msa1355490:CDS | 45.0% |
| ! | TGAGGAACCTCTGAAGGAAT+GGG | - | tig0014794:55751-55770 | Msa1355490:CDS | 45.0% |
| GAACCAGTGGCATTCTTAGG+TGG | - | tig0014794:55695-55714 | Msa1355490:CDS | 50.0% | |
| ATCCCACCTAAGAATGCCAC+TGG | + | tig0014794:55701-55720 | None:intergenic | 50.0% | |
| CTCGTAACCCATTCCTTCAG+AGG | + | tig0014794:55761-55780 | None:intergenic | 50.0% | |
| !! | CTCTGAAGGAATGGGTTACG+AGG | - | tig0014794:55759-55778 | Msa1355490:CDS | 50.0% |
| GAACCAGTGGCATTCTTAGG+TGG | - | tig0014794:55695-55714 | Msa1355490:CDS | 50.0% | |
| ATCCCACCTAAGAATGCCAC+TGG | + | tig0014794:55701-55720 | None:intergenic | 50.0% | |
| CTCGTAACCCATTCCTTCAG+AGG | + | tig0014794:55761-55780 | None:intergenic | 50.0% | |
| !! | CTCTGAAGGAATGGGTTACG+AGG | - | tig0014794:55759-55778 | Msa1355490:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0014794 | gene | 55695 | 55871 | 55695 | ID=Msa1355490;Name=Msa1355490 |
| tig0014794 | mRNA | 55695 | 55871 | 55695 | ID=Msa1355490-mRNA-1;Parent=Msa1355490;Name=Msa1355490-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|-1|0|1|-1|1|1|0|58 |
| tig0014794 | exon | 55695 | 55871 | 55695 | ID=Msa1355490-mRNA-1:exon:9483;Parent=Msa1355490-mRNA-1 |
| tig0014794 | CDS | 55695 | 55871 | 55695 | ID=Msa1355490-mRNA-1:cds;Parent=Msa1355490-mRNA-1 |
| Gene Sequence |
| Protein sequence |
