AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0126000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0126000 MtrunA17_Chr1g0188791 93.103 116 8 0 14 129 15 130 2.80e-74 217
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0126000 AT1G28150.1 60.227 88 32 2 45 129 36 123 1.19e-28 101
Msa0126000 AT1G28150.2 60.227 88 32 2 45 129 36 123 1.19e-28 101

Find 44 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCTTAATGAATATTAT+TGG 0.190749 1_3:-54860580 None:intergenic
CCAAATCAATCCGATTCTTA+TGG 0.247683 1_3:-54857275 None:intergenic
GCTTCAGTAGTTGATTCTTC+TGG 0.258189 1_3:-54860161 None:intergenic
TTCTTAGGTGGGATATTTGC+TGG 0.305119 1_3:+54860047 Msa0126000:CDS
CATATAGGACATAATAGTTC+TGG 0.312454 1_3:+54860320 Msa0126000:three_prime_UTR
CAGGAACCAGTGGCATTCTT+AGG 0.325742 1_3:+54860032 Msa0126000:intron
GGAATTAAAACCTATGCTTC+TGG 0.338871 1_3:-54857531 None:intergenic
TGGTAAATTGTCAACATTCA+TGG 0.342708 1_3:+54860368 Msa0126000:three_prime_UTR
ATTGTTTGCACCATTTGTTT+TGG 0.357688 1_3:+54860487 Msa0126000:three_prime_UTR
CAAATCAATCCGATTCTTAT+GGG 0.373950 1_3:-54857274 None:intergenic
ATTCCTTTCGAGTTGGTAAT+CGG 0.384706 1_3:+54857549 Msa0126000:CDS
CCATAAGAATCGGATTGATT+TGG 0.397964 1_3:+54857275 Msa0126000:five_prime_UTR
AGTAGTTGATTCTTCTGGAT+TGG 0.410215 1_3:-54860156 None:intergenic
TTAAAGATATCAAAGTTGTT+TGG 0.418131 1_3:+54860542 Msa0126000:three_prime_UTR
TGAAATTGAAGCAGAAATGT+AGG 0.423767 1_3:+54857465 Msa0126000:intron
AATTGTCAACATTCATGGCT+TGG 0.447143 1_3:+54860373 Msa0126000:three_prime_UTR
TTGACGAGAGGGTCTTGAGC+AGG 0.477747 1_3:-54857599 None:intergenic
TATTCAATTTCAATCTCTTG+TGG 0.483856 1_3:-54860188 None:intergenic
ACAAATTATTATTGTGCTGT+TGG 0.485885 1_3:-54860256 None:intergenic
TATCACCATCATCAATTTCG+CGG 0.495613 1_3:+54857492 Msa0126000:CDS
GGTTAAACTGCCAAAACAAA+TGG 0.497837 1_3:-54860497 None:intergenic
ATGAGGAACCTCTGAAGGAA+TGG 0.505614 1_3:+54860090 Msa0126000:CDS
AAAACAGCGCCCATAAGAAT+CGG 0.507763 1_3:+54857265 None:intergenic
TTTAAGGCTTGATTTGAATG+AGG 0.510298 1_3:+54860073 Msa0126000:CDS
GACAAGAATAACTATATAGC+AGG 0.512346 1_3:-54860518 None:intergenic
TGAGGAACCTCTGAAGGAAT+GGG 0.523835 1_3:+54860091 Msa0126000:CDS
TCTTCAACTGTCATGCACTC+AGG 0.533853 1_3:+54857383 Msa0126000:CDS
CTCGTAACCCATTCCTTCAG+AGG 0.539677 1_3:-54860098 None:intergenic
TTCTAATCTGCAGGAACCAG+TGG 0.542541 1_3:+54860022 Msa0126000:intron
GAACCAGTGGCATTCTTAGG+TGG 0.545711 1_3:+54860035 Msa0126000:CDS
TTTGAATGAGGAACCTCTGA+AGG 0.563194 1_3:+54860085 Msa0126000:CDS
TGACAGCTTCTTTGACGAGA+GGG 0.564005 1_3:-54857610 None:intergenic
CGAGTCCGCGAAATTGATGA+TGG 0.565396 1_3:-54857497 None:intergenic
ATCCCACCTAAGAATGCCAC+TGG 0.574031 1_3:-54860038 None:intergenic
TGAGTGCATGACAGTTGAAG+AGG 0.582968 1_3:-54857381 None:intergenic
CTAGTGTGGTAGTTGCGAGT+TGG 0.583653 1_3:+54857307 Msa0126000:five_prime_UTR
CTCTGAAGGAATGGGTTACG+AGG 0.596910 1_3:+54860099 Msa0126000:CDS
AACCAGTGGCATTCTTAGGT+GGG 0.598806 1_3:+54860036 Msa0126000:CDS
TCTCGTCAAAGAAGCTGTCA+AGG 0.609141 1_3:+54857613 Msa0126000:CDS
ATCAACAGAACCAGAAGCAT+AGG 0.626738 1_3:+54857521 Msa0126000:CDS
TTTGGTAGTTGCTGCTAGTG+TGG 0.630089 1_3:+54857293 Msa0126000:five_prime_UTR
TTGACAGCTTCTTTGACGAG+AGG 0.633404 1_3:-54857611 None:intergenic
ACTCCGATTACCAACTCGAA+AGG 0.638817 1_3:-54857552 None:intergenic
GTAGTTGCGAGTTGGAACAA+AGG 0.679107 1_3:+54857315 Msa0126000:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTTAGCTTATTTAT+TGG + chr1_3:54857705-54857724 Msa0126000:intron 10.0%
!! AGAAAAAATTGTTAATAATA+TGG + chr1_3:54858480-54858499 Msa0126000:intron 10.0%
!!! TGAGATTTTAAATTAATATA+AGG + chr1_3:54858594-54858613 Msa0126000:intron 10.0%
!!! AATTAATATAAGGATAATTT+TGG + chr1_3:54858604-54858623 Msa0126000:intron 10.0%
!! AAAATCTTAATGAATATTAT+TGG - chr1_3:54860583-54860602 None:intergenic 10.0%
!! ATAAAGAGGAATAATAATAA+CGG - chr1_3:54857410-54857429 None:intergenic 15.0%
!!! ATTTTAATTAAAAGTGTGTT+TGG + chr1_3:54858274-54858293 Msa0126000:intron 15.0%
!!! CCTTATTTAATTAAGTTTAA+GGG - chr1_3:54858345-54858364 None:intergenic 15.0%
!!! TCCTTATTTAATTAAGTTTA+AGG - chr1_3:54858346-54858365 None:intergenic 15.0%
!!! TTTTACTATTATTAGGTTAT+AGG - chr1_3:54858534-54858553 None:intergenic 15.0%
!!! AAACAACTTTTACTATTATT+AGG - chr1_3:54858541-54858560 None:intergenic 15.0%
!! GAGAAATAAATAAATTGTAA+TGG - chr1_3:54859033-54859052 None:intergenic 15.0%
!!! TCAAGTTATTCTAAAATTTT+GGG - chr1_3:54859474-54859493 None:intergenic 15.0%
!!! TTCAAGTTATTCTAAAATTT+TGG - chr1_3:54859475-54859494 None:intergenic 15.0%
!!! TATTTTCAAGTGTTTTATTT+TGG + chr1_3:54860442-54860461 Msa0126000:three_prime_UTR 15.0%
!! GTTATTATTATTCCTCTTTA+TGG + chr1_3:54857409-54857428 Msa0126000:intron 20.0%
!! AGCTAAAAATAAATACAAGA+AGG - chr1_3:54857698-54857717 None:intergenic 20.0%
!! ACAAAATTAACAACTAACAT+TGG + chr1_3:54857995-54858014 Msa0126000:intron 20.0%
!!! ATACAAATAGAGTTTTATGT+CGG + chr1_3:54858076-54858095 Msa0126000:intron 20.0%
!! CCCTTAAACTTAATTAAATA+AGG + chr1_3:54858342-54858361 Msa0126000:intron 20.0%
!!! TTTGGTTCAATTTTTAGAAA+GGG - chr1_3:54858660-54858679 None:intergenic 20.0%
!!! GATTTTTAATTTGAAGTGTT+TGG - chr1_3:54858678-54858697 None:intergenic 20.0%
!!! ATTTATTTCTCAGTTCTTTT+TGG + chr1_3:54859042-54859061 Msa0126000:intron 20.0%
!!! TTTATTTCTCAGTTCTTTTT+GGG + chr1_3:54859043-54859062 Msa0126000:intron 20.0%
!!! TTTGAAGTTCTGTTATTTTT+GGG - chr1_3:54859087-54859106 None:intergenic 20.0%
!! TGATTCTACTATGATTTATT+AGG - chr1_3:54859183-54859202 None:intergenic 20.0%
!! TTGATAAATAAAGAAGTTCA+TGG - chr1_3:54859678-54859697 None:intergenic 20.0%
!!! CAATTTTATCACAATTCTAT+AGG + chr1_3:54859903-54859922 Msa0126000:intron 20.0%
!!! TGCAAGTTTTAAAGAAATAA+AGG - chr1_3:54860223-54860242 None:intergenic 20.0%
!!! TTATCACACATTTTTCATAT+AGG + chr1_3:54860305-54860324 Msa0126000:three_prime_UTR 20.0%
!!! TTTTGGAAATCAAATACAAA+AGG + chr1_3:54860459-54860478 Msa0126000:three_prime_UTR 20.0%
!! TTAAAGATATCAAAGTTGTT+TGG + chr1_3:54860542-54860561 Msa0126000:three_prime_UTR 20.0%
! ATGTTCAGCTTATTTCAATT+TGG + chr1_3:54857739-54857758 Msa0126000:intron 25.0%
! GCTATATATAAGAGAAAGTT+TGG + chr1_3:54857785-54857804 Msa0126000:intron 25.0%
!! AGCTGATTTAGTTCAAAATT+TGG - chr1_3:54857835-54857854 None:intergenic 25.0%
!!! TTGGTTCAATTTTTAGAAAG+GGG - chr1_3:54858659-54858678 None:intergenic 25.0%
!!! GTTTGGTTCAATTTTTAGAA+AGG - chr1_3:54858661-54858680 None:intergenic 25.0%
! TAAACATAACCTGACTTATA+GGG - chr1_3:54858740-54858759 None:intergenic 25.0%
! ATAAACATAACCTGACTTAT+AGG - chr1_3:54858741-54858760 None:intergenic 25.0%
! TTGTTCATGAATCAATTGTA+AGG - chr1_3:54858770-54858789 None:intergenic 25.0%
!! AATTTCAGTTTTACACCAAA+AGG - chr1_3:54858900-54858919 None:intergenic 25.0%
!!! TTCTTTTTGGGATAAAAAGA+GGG + chr1_3:54859055-54859074 Msa0126000:intron 25.0%
!!! CTTTGAAGTTCTGTTATTTT+TGG - chr1_3:54859088-54859107 None:intergenic 25.0%
! TGTCTTAGTTAATATGGATT+AGG - chr1_3:54859209-54859228 None:intergenic 25.0%
!! TGATGATGTCTTAGTTAATA+TGG - chr1_3:54859215-54859234 None:intergenic 25.0%
! ACTACAAAGCTCTATAATAT+AGG + chr1_3:54859437-54859456 Msa0126000:intron 25.0%
! AATGAAAAAGTTCTCACAAA+GGG - chr1_3:54859602-54859621 None:intergenic 25.0%
!!! ATTGCTAGGCTTTAAAAATT+TGG + chr1_3:54859961-54859980 Msa0126000:intron 25.0%
! TATTCAATTTCAATCTCTTG+TGG - chr1_3:54860191-54860210 None:intergenic 25.0%
! ACAAATTATTATTGTGCTGT+TGG - chr1_3:54860259-54860278 None:intergenic 25.0%
CAAATCAATCCGATTCTTAT+GGG - chr1_3:54857277-54857296 None:intergenic 30.0%
CAATTGAATGAAGAGAAAAC+AGG - chr1_3:54857437-54857456 None:intergenic 30.0%
TGAAATTGAAGCAGAAATGT+AGG + chr1_3:54857465-54857484 Msa0126000:intron 30.0%
TACAAGAAGGAGCTAAAATA+TGG - chr1_3:54857685-54857704 None:intergenic 30.0%
GAATATAAAAAATACGCCTC+AGG + chr1_3:54857761-54857780 Msa0126000:intron 30.0%
AAAATCTAACAAGATCAGCT+AGG + chr1_3:54857888-54857907 Msa0126000:intron 30.0%
AATTAAGCAATTACACTTGC+AGG - chr1_3:54858047-54858066 None:intergenic 30.0%
! AATTGATAACACATGAGACA+TGG - chr1_3:54858137-54858156 None:intergenic 30.0%
!! TTCAATTTTTAGAAAGGGGA+GGG - chr1_3:54858655-54858674 None:intergenic 30.0%
AAAACTGTCAGTGGAAAATA+TGG + chr1_3:54858807-54858826 Msa0126000:intron 30.0%
! TTTTCAGTGGTAACATAACA+TGG - chr1_3:54858856-54858875 None:intergenic 30.0%
! TTTTGCATCACAATTTTCAG+TGG - chr1_3:54858869-54858888 None:intergenic 30.0%
!!! GTTCTTTTTGGGATAAAAAG+AGG + chr1_3:54859054-54859073 Msa0126000:intron 30.0%
AACTTCAAAGCAATTAAACG+TGG + chr1_3:54859098-54859117 Msa0126000:intron 30.0%
! GAATAGAATGTTGCTTGAAA+TGG - chr1_3:54859285-54859304 None:intergenic 30.0%
!! TATAGAGCTTTGTAGTGTTT+TGG - chr1_3:54859433-54859452 None:intergenic 30.0%
! CTAATCATGGACTGTAATTT+AGG + chr1_3:54859560-54859579 Msa0126000:intron 30.0%
! ATGGACTGTAATTTAGGAAT+TGG + chr1_3:54859566-54859585 Msa0126000:intron 30.0%
GAATGAAAAAGTTCTCACAA+AGG - chr1_3:54859603-54859622 None:intergenic 30.0%
! TCATACCAGTGTTATTACAA+GGG - chr1_3:54859726-54859745 None:intergenic 30.0%
! TTCATACCAGTGTTATTACA+AGG - chr1_3:54859727-54859746 None:intergenic 30.0%
TATGTTAACAAGTTGCTATC+TGG + chr1_3:54859833-54859852 Msa0126000:intron 30.0%
!! TCTTGTTATTTCTAATCTGC+AGG + chr1_3:54860013-54860032 Msa0126000:intron 30.0%
!! GGATATTTGCTGGTATTTTA+AGG + chr1_3:54860057-54860076 Msa0126000:CDS 30.0%
! TTTAAGGCTTGATTTGAATG+AGG + chr1_3:54860073-54860092 Msa0126000:CDS 30.0%
CATATAGGACATAATAGTTC+TGG + chr1_3:54860320-54860339 Msa0126000:three_prime_UTR 30.0%
! CTTTAAAAAGACGAAGACAA+AGG - chr1_3:54860347-54860366 None:intergenic 30.0%
!! TGTCTTCGTCTTTTTAAAGA+TGG + chr1_3:54860348-54860367 Msa0126000:three_prime_UTR 30.0%
TGGTAAATTGTCAACATTCA+TGG + chr1_3:54860368-54860387 Msa0126000:three_prime_UTR 30.0%
! ATTGTTTGCACCATTTGTTT+TGG + chr1_3:54860487-54860506 Msa0126000:three_prime_UTR 30.0%
GACAAGAATAACTATATAGC+AGG - chr1_3:54860521-54860540 None:intergenic 30.0%
CCAAATCAATCCGATTCTTA+TGG - chr1_3:54857278-54857297 None:intergenic 35.0%
CCATAAGAATCGGATTGATT+TGG + chr1_3:54857275-54857294 Msa0126000:five_prime_UTR 35.0%
AGAAAACAGGAACCATAAAG+AGG - chr1_3:54857424-54857443 None:intergenic 35.0%
TATCACCATCATCAATTTCG+CGG + chr1_3:54857492-54857511 Msa0126000:CDS 35.0%
GGAATTAAAACCTATGCTTC+TGG - chr1_3:54857534-54857553 None:intergenic 35.0%
!!! GGTTTTAATTCCTTTCGAGT+TGG + chr1_3:54857542-54857561 Msa0126000:CDS 35.0%
!! ATTCCTTTCGAGTTGGTAAT+CGG + chr1_3:54857549-54857568 Msa0126000:CDS 35.0%
TCTCTTATATATAGCACCTG+AGG - chr1_3:54857780-54857799 None:intergenic 35.0%
! AAAAGTGTGTTTGGTTCACA+AGG + chr1_3:54858283-54858302 Msa0126000:intron 35.0%
! AAAGTGTGTTTGGTTCACAA+GGG + chr1_3:54858284-54858303 Msa0126000:intron 35.0%
!! TCAATTTTTAGAAAGGGGAG+GGG - chr1_3:54858654-54858673 None:intergenic 35.0%
!! GTTCAATTTTTAGAAAGGGG+AGG - chr1_3:54858656-54858675 None:intergenic 35.0%
TCAGCATATAAAACTGTCAG+TGG + chr1_3:54858798-54858817 Msa0126000:intron 35.0%
! TGCAAAAGTCATGTTCCTTT+TGG + chr1_3:54858882-54858901 Msa0126000:intron 35.0%
AAAGCAATTAAACGTGGAGT+AGG + chr1_3:54859104-54859123 Msa0126000:intron 35.0%
GTTGGATTAAGCTTAACATG+GGG - chr1_3:54859520-54859539 None:intergenic 35.0%
GGTTGGATTAAGCTTAACAT+GGG - chr1_3:54859521-54859540 None:intergenic 35.0%
TTAAGCTTAATCCAACCCAA+AGG + chr1_3:54859524-54859543 Msa0126000:intron 35.0%
TACAGTCCATGATTAGATTG+TGG - chr1_3:54859556-54859575 None:intergenic 35.0%
TAAAGAAGTTCATGGTGAAC+TGG - chr1_3:54859670-54859689 None:intergenic 35.0%
ATGAACCCTTGTAATAACAC+TGG + chr1_3:54859718-54859737 Msa0126000:intron 35.0%
TTAACAAGTTGCTATCTGGA+TGG + chr1_3:54859837-54859856 Msa0126000:intron 35.0%
TAGCTACATCATAAGAACCA+GGG - chr1_3:54859879-54859898 None:intergenic 35.0%
! AGTAGTTGATTCTTCTGGAT+TGG - chr1_3:54860159-54860178 None:intergenic 35.0%
AATTGTCAACATTCATGGCT+TGG + chr1_3:54860373-54860392 Msa0126000:three_prime_UTR 35.0%
GGTTAAACTGCCAAAACAAA+TGG - chr1_3:54860500-54860519 None:intergenic 35.0%
! ATCAACAGAACCAGAAGCAT+AGG + chr1_3:54857521-54857540 Msa0126000:CDS 40.0%
ATCTAACAAGATCAGCTAGG+AGG + chr1_3:54857891-54857910 Msa0126000:intron 40.0%
TCTAACAAGATCAGCTAGGA+GGG + chr1_3:54857892-54857911 Msa0126000:intron 40.0%
GTTAGAGAGTGGAGTGATAA+CGG + chr1_3:54857948-54857967 Msa0126000:intron 40.0%
TGAGACATGGCACAACTATA+TGG - chr1_3:54858124-54858143 None:intergenic 40.0%
!! AGGGAAGGAATTTTAACGGA+GGG + chr1_3:54858236-54858255 Msa0126000:intron 40.0%
! AAGTGTGTTTGGTTCACAAG+GGG + chr1_3:54858285-54858304 Msa0126000:intron 40.0%
TAACAACACGATGTAGACGA+TGG + chr1_3:54858400-54858419 Msa0126000:intron 40.0%
CGATGTAGACGATGGAATAA+CGG + chr1_3:54858408-54858427 Msa0126000:intron 40.0%
!!! TTTGGTTCGAGAGTTTTGGA+GGG - chr1_3:54858713-54858732 None:intergenic 40.0%
TCCAAAACTCTCGAACCAAA+CGG + chr1_3:54858713-54858732 Msa0126000:intron 40.0%
TTTCTCTTAGCTGATCTCGT+TGG - chr1_3:54859134-54859153 None:intergenic 40.0%
GAAACAAACCAACTAGAGCA+TGG + chr1_3:54859150-54859169 Msa0126000:intron 40.0%
GAGAATTTCCATGCTCTAGT+TGG - chr1_3:54859161-54859180 None:intergenic 40.0%
GACATCATCAGTACATGAGT+TGG + chr1_3:54859225-54859244 Msa0126000:intron 40.0%
AACGATTTCTCCAATGACTG+TGG - chr1_3:54859332-54859351 None:intergenic 40.0%
AGTCATTGGAGAAATCGTTG+AGG + chr1_3:54859333-54859352 Msa0126000:intron 40.0%
TAACATGGGGCTTCTAATAG+TGG - chr1_3:54859507-54859526 None:intergenic 40.0%
GGGTTGGATTAAGCTTAACA+TGG - chr1_3:54859522-54859541 None:intergenic 40.0%
AGGTAGCCACAATCTAATCA+TGG + chr1_3:54859547-54859566 Msa0126000:intron 40.0%
TACATCATAAGAACCAGGGA+TGG - chr1_3:54859875-54859894 None:intergenic 40.0%
GTAGCTACATCATAAGAACC+AGG - chr1_3:54859880-54859899 None:intergenic 40.0%
TTCTTAGGTGGGATATTTGC+TGG + chr1_3:54860047-54860066 Msa0126000:CDS 40.0%
TTTGAATGAGGAACCTCTGA+AGG + chr1_3:54860085-54860104 Msa0126000:CDS 40.0%
! GCTTCAGTAGTTGATTCTTC+TGG - chr1_3:54860164-54860183 None:intergenic 40.0%
! TTTGGTAGTTGCTGCTAGTG+TGG + chr1_3:54857293-54857312 Msa0126000:five_prime_UTR 45.0%
! GTAGTTGCGAGTTGGAACAA+AGG + chr1_3:54857315-54857334 Msa0126000:five_prime_UTR 45.0%
TGAGTGCATGACAGTTGAAG+AGG - chr1_3:54857384-54857403 None:intergenic 45.0%
TCTTCAACTGTCATGCACTC+AGG + chr1_3:54857383-54857402 Msa0126000:CDS 45.0%
ACTCCGATTACCAACTCGAA+AGG - chr1_3:54857555-54857574 None:intergenic 45.0%
TGACAGCTTCTTTGACGAGA+GGG - chr1_3:54857613-54857632 None:intergenic 45.0%
TTGACAGCTTCTTTGACGAG+AGG - chr1_3:54857614-54857633 None:intergenic 45.0%
TCTCGTCAAAGAAGCTGTCA+AGG + chr1_3:54857613-54857632 Msa0126000:CDS 45.0%
CTAACAAGATCAGCTAGGAG+GGG + chr1_3:54857893-54857912 Msa0126000:intron 45.0%
GAAGTGGAAGAGTTAGAGAG+TGG + chr1_3:54857937-54857956 Msa0126000:intron 45.0%
!! TGGAGTGATAACGGTGTTCT+CGG + chr1_3:54857957-54857976 Msa0126000:intron 45.0%
TTAGTTGTGTCCTGTGACCT+TGG + chr1_3:54858179-54858198 Msa0126000:intron 45.0%
ATTCTCGAACATCGCAACCA+AGG - chr1_3:54858199-54858218 None:intergenic 45.0%
GGTTGCGATGTTCGAGAATT+AGG + chr1_3:54858200-54858219 Msa0126000:intron 45.0%
TGCGATGTTCGAGAATTAGG+AGG + chr1_3:54858203-54858222 Msa0126000:intron 45.0%
GCGATGTTCGAGAATTAGGA+GGG + chr1_3:54858204-54858223 Msa0126000:intron 45.0%
CGATGTTCGAGAATTAGGAG+GGG + chr1_3:54858205-54858224 Msa0126000:intron 45.0%
GATGTTCGAGAATTAGGAGG+GGG + chr1_3:54858206-54858225 Msa0126000:intron 45.0%
ATGTTCGAGAATTAGGAGGG+GGG + chr1_3:54858207-54858226 Msa0126000:intron 45.0%
! GGGGAGGGAAGGAATTTTAA+CGG + chr1_3:54858232-54858251 Msa0126000:intron 45.0%
!! GAGGGAAGGAATTTTAACGG+AGG + chr1_3:54858235-54858254 Msa0126000:intron 45.0%
!! GGGAAGGAATTTTAACGGAG+GGG + chr1_3:54858237-54858256 Msa0126000:intron 45.0%
!! AAGGAATTTTAACGGAGGGG+AGG + chr1_3:54858240-54858259 Msa0126000:intron 45.0%
!! AGGAATTTTAACGGAGGGGA+GGG + chr1_3:54858241-54858260 Msa0126000:intron 45.0%
!! TTTTTAGAAAGGGGAGGGGA+GGG - chr1_3:54858650-54858669 None:intergenic 45.0%
!! ATTTTTAGAAAGGGGAGGGG+AGG - chr1_3:54858651-54858670 None:intergenic 45.0%
!!! TTGGTTCGAGAGTTTTGGAG+GGG - chr1_3:54858712-54858731 None:intergenic 45.0%
!! GTTTGGTTCGAGAGTTTTGG+AGG - chr1_3:54858714-54858733 None:intergenic 45.0%
!! TCCGTTTGGTTCGAGAGTTT+TGG - chr1_3:54858717-54858736 None:intergenic 45.0%
CACACGAGTCATGACAGAAA+TGG - chr1_3:54858930-54858949 None:intergenic 45.0%
! CATGAGTTGGCTTCACGATA+TGG + chr1_3:54859238-54859257 Msa0126000:intron 45.0%
! ATGTTGCTTGAAATGGCGAG+AGG - chr1_3:54859278-54859297 None:intergenic 45.0%
! TGTAGTTAGCCTGGTCTGAT+GGG - chr1_3:54859404-54859423 None:intergenic 45.0%
! TTGTAGTTAGCCTGGTCTGA+TGG - chr1_3:54859405-54859424 None:intergenic 45.0%
TGGAGAGATTGTAGTTAGCC+TGG - chr1_3:54859413-54859432 None:intergenic 45.0%
AGCTTAATCCAACCCAAAGG+AGG + chr1_3:54859527-54859546 Msa0126000:intron 45.0%
AGATTGTGGCTACCTCCTTT+GGG - chr1_3:54859542-54859561 None:intergenic 45.0%
TAGATTGTGGCTACCTCCTT+TGG - chr1_3:54859543-54859562 None:intergenic 45.0%
! TGGTGAACTGGTGAGCTTTT+CGG - chr1_3:54859658-54859677 None:intergenic 45.0%
! GTAAGTTGACTTACCATCCC+TGG + chr1_3:54859859-54859878 Msa0126000:intron 45.0%
TTCTAATCTGCAGGAACCAG+TGG + chr1_3:54860022-54860041 Msa0126000:intron 45.0%
AACCAGTGGCATTCTTAGGT+GGG + chr1_3:54860036-54860055 Msa0126000:CDS 45.0%
! ATGAGGAACCTCTGAAGGAA+TGG + chr1_3:54860090-54860109 Msa0126000:CDS 45.0%
! TGAGGAACCTCTGAAGGAAT+GGG + chr1_3:54860091-54860110 Msa0126000:CDS 45.0%
!!! AGTTTTTTTTTTTTTTTTAA+CGG - chr1_3:54858021-54858040 None:intergenic 5.0%
CTAGTGTGGTAGTTGCGAGT+TGG + chr1_3:54857307-54857326 Msa0126000:five_prime_UTR 50.0%
CGAGTCCGCGAAATTGATGA+TGG - chr1_3:54857500-54857519 None:intergenic 50.0%
AGCTAGGAGGGGAATGAAGT+TGG + chr1_3:54857904-54857923 Msa0126000:intron 50.0%
!! AGTTGGCTTGGTGGTTGAAG+TGG + chr1_3:54857921-54857940 Msa0126000:intron 50.0%
AACATCGCAACCAAGGTCAC+AGG - chr1_3:54858192-54858211 None:intergenic 50.0%
TGTTCGAGAATTAGGAGGGG+GGG + chr1_3:54858208-54858227 Msa0126000:intron 50.0%
!! GGAATTTTAACGGAGGGGAG+GGG + chr1_3:54858242-54858261 Msa0126000:intron 50.0%
! TGTGTTTGGTTCACAAGGGG+AGG + chr1_3:54858288-54858307 Msa0126000:intron 50.0%
! GTGTTTGGTTCACAAGGGGA+GGG + chr1_3:54858289-54858308 Msa0126000:intron 50.0%
! TGTTTGGTTCACAAGGGGAG+GGG + chr1_3:54858290-54858309 Msa0126000:intron 50.0%
!! TTTTAGAAAGGGGAGGGGAG+GGG - chr1_3:54858649-54858668 None:intergenic 50.0%
!!! TTCGAGAGTTTTGGAGGGGA+GGG - chr1_3:54858708-54858727 None:intergenic 50.0%
CCTGACTTATAGGGTCCGTT+TGG - chr1_3:54858731-54858750 None:intergenic 50.0%
CCAAACGGACCCTATAAGTC+AGG + chr1_3:54858728-54858747 Msa0126000:intron 50.0%
CTTGAAATGGCGAGAGGTGA+AGG - chr1_3:54859272-54859291 None:intergenic 50.0%
! CTCGGTGGAGTTTGGTGAAA+TGG - chr1_3:54859307-54859326 None:intergenic 50.0%
TCTCCAATGACTGTGGAGCT+CGG - chr1_3:54859325-54859344 None:intergenic 50.0%
! GGTGAACTGGTGAGCTTTTC+GGG - chr1_3:54859657-54859676 None:intergenic 50.0%
CTAGCAATGAACGTTCCTGC+GGG - chr1_3:54859949-54859968 None:intergenic 50.0%
CCTAGCAATGAACGTTCCTG+CGG - chr1_3:54859950-54859969 None:intergenic 50.0%
CAGGAACCAGTGGCATTCTT+AGG + chr1_3:54860032-54860051 Msa0126000:intron 50.0%
GAACCAGTGGCATTCTTAGG+TGG + chr1_3:54860035-54860054 Msa0126000:CDS 50.0%
ATCCCACCTAAGAATGCCAC+TGG - chr1_3:54860041-54860060 None:intergenic 50.0%
CTCGTAACCCATTCCTTCAG+AGG - chr1_3:54860101-54860120 None:intergenic 50.0%
!! CTCTGAAGGAATGGGTTACG+AGG + chr1_3:54860099-54860118 Msa0126000:CDS 50.0%
TTGACGAGAGGGTCTTGAGC+AGG - chr1_3:54857602-54857621 None:intergenic 55.0%
GGAGGGGAATGAAGTTGGCT+TGG + chr1_3:54857909-54857928 Msa0126000:intron 55.0%
!! GGGGAATGAAGTTGGCTTGG+TGG + chr1_3:54857912-54857931 Msa0126000:intron 55.0%
!! ATTTTAACGGAGGGGAGGGG+AGG + chr1_3:54858245-54858264 Msa0126000:intron 55.0%
! TTTTAACGGAGGGGAGGGGA+GGG + chr1_3:54858246-54858265 Msa0126000:intron 55.0%
!!! TCGAGAGTTTTGGAGGGGAG+GGG - chr1_3:54858707-54858726 None:intergenic 55.0%
!!! GTTCGAGAGTTTTGGAGGGG+AGG - chr1_3:54858709-54858728 None:intergenic 55.0%
AGCTCGAAACCCATCAGACC+AGG + chr1_3:54859392-54859411 Msa0126000:intron 55.0%
TGTGGCTACCTCCTTTGGGT+TGG - chr1_3:54859538-54859557 None:intergenic 55.0%
AAGCATTCTGTTCAGCCCGC+AGG + chr1_3:54859931-54859950 Msa0126000:intron 55.0%
CCGCAGGAACGTTCATTGCT+AGG + chr1_3:54859947-54859966 Msa0126000:intron 55.0%
TCGAGAATTAGGAGGGGGGG+AGG + chr1_3:54858211-54858230 Msa0126000:intron 60.0%
CGAGAATTAGGAGGGGGGGA+GGG + chr1_3:54858212-54858231 Msa0126000:intron 60.0%
GAGAATTAGGAGGGGGGGAG+GGG + chr1_3:54858213-54858232 Msa0126000:intron 60.0%
TTTAACGGAGGGGAGGGGAG+GGG + chr1_3:54858247-54858266 Msa0126000:intron 60.0%
TTGGTTCACAAGGGGAGGGG+AGG + chr1_3:54858293-54858312 Msa0126000:intron 60.0%
TGGTTCACAAGGGGAGGGGA+GGG + chr1_3:54858294-54858313 Msa0126000:intron 60.0%
!!! AGTTTTGGAGGGGAGGGGAG+GGG - chr1_3:54858702-54858721 None:intergenic 60.0%
!!! GAGTTTTGGAGGGGAGGGGA+GGG - chr1_3:54858703-54858722 None:intergenic 60.0%
!!! AGAGTTTTGGAGGGGAGGGG+AGG - chr1_3:54858704-54858723 None:intergenic 60.0%
! CTGTGGAGCTCGGTGGAGTT+TGG - chr1_3:54859315-54859334 None:intergenic 60.0%
CCAATGACTGTGGAGCTCGG+TGG - chr1_3:54859322-54859341 None:intergenic 60.0%
CCACCGAGCTCCACAGTCAT+TGG + chr1_3:54859319-54859338 Msa0126000:intron 60.0%
AATTAGGAGGGGGGGAGGGG+AGG + chr1_3:54858216-54858235 Msa0126000:intron 65.0%
ATTAGGAGGGGGGGAGGGGA+GGG + chr1_3:54858217-54858236 Msa0126000:intron 65.0%
! GGAGGGGGGGAGGGGAGGGA+AGG + chr1_3:54858221-54858240 Msa0126000:intron 80.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 54857272 54860625 54857272 ID=Msa0126000;Name=Msa0126000
chr1_3 mRNA 54857272 54860625 54857272 ID=Msa0126000-mRNA-1;Parent=Msa0126000;Name=Msa0126000-mRNA-1;_AED=0.10;_eAED=0.10;_QI=76|1|1|1|1|1|3|414|129
chr1_3 exon 54857272 54857404 54857272 ID=Msa0126000-mRNA-1:exon:16915;Parent=Msa0126000-mRNA-1
chr1_3 exon 54857479 54857634 54857479 ID=Msa0126000-mRNA-1:exon:16916;Parent=Msa0126000-mRNA-1
chr1_3 exon 54860035 54860625 54860035 ID=Msa0126000-mRNA-1:exon:16917;Parent=Msa0126000-mRNA-1
chr1_3 five_prime_UTR 54857272 54857347 54857272 ID=Msa0126000-mRNA-1:five_prime_utr;Parent=Msa0126000-mRNA-1
chr1_3 CDS 54857348 54857404 54857348 ID=Msa0126000-mRNA-1:cds;Parent=Msa0126000-mRNA-1
chr1_3 CDS 54857479 54857634 54857479 ID=Msa0126000-mRNA-1:cds;Parent=Msa0126000-mRNA-1
chr1_3 CDS 54860035 54860211 54860035 ID=Msa0126000-mRNA-1:cds;Parent=Msa0126000-mRNA-1
chr1_3 three_prime_UTR 54860212 54860625 54860212 ID=Msa0126000-mRNA-1:three_prime_utr;Parent=Msa0126000-mRNA-1
Gene Sequence

>Msa0126000

ATGTCTCTTCTCTTTACTTCGTCTTCTGTTTCTCCTCTTCAACTGTCATGCACTCAGAAATGTAGGTTTTTATCACCATCATCAATTTCGCGGACTCGAATCAACAGAACCAGAAGCATAGGTTTTAATTCCTTTCGAGTTGGTAATCGGAGTCGAATTACTGCTTTTTTCTTCAATCCTGCTCAAGACCCTCTCGTCAAAGAAGCTGTCAAGGAACCAGTGGCATTCTTAGGTGGGATATTTGCTGGTATTTTAAGGCTTGATTTGAATGAGGAACCTCTGAAGGAATGGGTTACGAGGACTGTTGAAGCTTCTGATATTAGTGAAGAAGAAACCAATCCAGAAGAATCAACTACTGAAGCTGCTCCACAAGAGATTGAAATTGAATAA
Protein sequence

>Msa0126000

MSLLFTSSSVSPLQLSCTQKCRFLSPSSISRTRINRTRSIGFNSFRVGNRSRITAFFFNPAQDPLVKEAVKEPVAFLGGIFAGILRLDLNEEPLKEWVTRTVEASDISEEETNPEESTTEAAPQEIEIE*