AlfalfaGEDB | Msa1317380

M. sativa cultivar zhongmu-4 / Msa1317380

KEGG: K18121
GO: GO:0003674 GO:0003824 GO:0003858 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0016491 GO:0016614 GO:0016616 GO:0035064 GO:0042393 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0055114 GO:0140030 GO:0140034
PFAM: PF03446.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1317380	sp\|Q9LSV0\|GLYR1_ARATH	77.483	302	50	2	1	301	1	285	2.38e-167	468

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1317380	I3SHU4	94.020	301	1	1	1	301	1	284	0.0	559

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001510	Msa1317380	0.819088	1.395523e-52	-8.615850e-47
Msa0002690	Msa1317380	0.802241	6.158449e-49	-8.615850e-47
Msa0003340	Msa1317380	0.818458	1.939689e-52	-8.615850e-47
Msa0004180	Msa1317380	0.847247	1.304955e-59	-8.615850e-47
Msa0005190	Msa1317380	0.833002	6.848552e-56	-8.615850e-47
Msa0009210	Msa1317380	0.829394	5.274727e-55	-8.615850e-47
Msa0011540	Msa1317380	0.812205	4.766637e-51	-8.615850e-47
Msa0016830	Msa1317380	0.819644	1.042520e-52	-8.615850e-47
Msa0018110	Msa1317380	0.816972	4.198141e-52	-8.615850e-47
Msa0018610	Msa1317380	0.838246	3.222483e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1317380	MtrunA17_Chr8g0347681	94.020	301	1	1	1	301	1	284	0.0	559
Msa1317380	MtrunA17_Chr2g0330111	53.488	301	122	2	2	301	47	330	3.86e-107	315
Msa1317380	MtrunA17_Chr8g0389841	28.283	297	193	4	2	296	37	315	3.98e-37	134
Msa1317380	MtrunA17_Chr8g0380761	27.703	296	190	5	2	291	15	292	4.43e-31	118
Msa1317380	MtrunA17_Chr2g0292541	27.070	314	192	5	2	295	41	337	5.37e-23	97.1
Msa1317380	MtrunA17_Chr6g0478521	25.806	310	198	7	2	300	326	614	2.41e-17	82.8
Msa1317380	MtrunA17_Chr6g0478521	24.138	290	182	6	3	280	7	270	4.15e-14	72.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1317380	AT3G25530.1	77.483	302	50	2	1	301	1	285	2.41e-168	468
Msa1317380	AT3G25530.2	74.086	301	51	2	1	301	1	274	2.42e-159	445
Msa1317380	AT3G25530.3	75.225	222	37	2	1	221	1	205	8.37e-118	337
Msa1317380	AT1G17650.2	54.636	302	119	2	1	301	8	292	8.84e-109	318
Msa1317380	AT1G17650.1	54.636	302	119	2	1	301	67	351	2.68e-108	319
Msa1317380	AT4G29120.1	29.293	297	190	4	2	296	39	317	1.68e-35	130
Msa1317380	AT4G20930.1	31.429	315	177	8	3	296	40	336	7.05e-28	110
Msa1317380	AT1G18270.1	27.508	309	194	7	2	300	323	611	4.32e-22	97.1
Msa1317380	AT1G18270.1	23.288	292	202	6	3	290	5	278	7.21e-13	69.7
Msa1317380	AT1G18270.2	27.508	309	194	7	2	300	324	612	4.32e-22	97.1

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Find 103 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGAGATTGGATTTCT+GGG	0.442385	8_4:+18606812	Msa1317380:exon
AAACAGTGACTTTGAAGCCA+TGG	0.555473	8_4:-18606877	None:intergenic
AAAGCCAGAAGCATGGGGTT+GGG	0.482519	8_4:+18610616	Msa1317380:CDS
AACGGCATGTTTAAATTGAA+AGG	0.477158	8_4:+18610164	Msa1317380:CDS
AAGGAAGGGTCTTTATCGAG+AGG	0.645360	8_4:+18606729	Msa1317380:five_prime_UTR
AATGAAACTTGTTGTCAACA+TGG	0.623343	8_4:+18609442	Msa1317380:CDS
ACAGTCATTTCATATATATG+TGG	0.498050	8_4:-18610761	None:intergenic
ACTAGTTGGCCATCTTCTGC+AGG	0.484133	8_4:-18608084	None:intergenic
ACTCCAGTGTAATAGTATTT+GGG	0.415175	8_4:-18610993	None:intergenic
ACTTCCAAATTATGAACAGA+AGG	0.583359	8_4:-18609497	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAATATATCACAGCCATTAA+TGG	-	chr8_4:18606696-18606715	None:intergenic	30.0%
TTTGCAACTAAATTCACTGT+TGG	-	chr8_4:18606794-18606813	None:intergenic	30.0%
TTAGTTGCAAAAATGGAGAT+TGG	+	chr8_4:18606803-18606822	Msa1317380:exon	30.0%
AAAAATGGAGATTGGATTTC+TGG	+	chr8_4:18606811-18606830	Msa1317380:exon	30.0%
AAAATGGAGATTGGATTTCT+GGG	+	chr8_4:18606812-18606831	Msa1317380:exon	30.0%
ACATCAAGGATAAATTTAGC+AGG	-	chr8_4:18607245-18607264	None:intergenic	30.0%
CTTGAGCAAATTAATGGAAA+AGG	+	chr8_4:18607346-18607365	Msa1317380:CDS	30.0%
CAATCTACGAAAAAGAGAAA+AGG	-	chr8_4:18607483-18607502	None:intergenic	30.0%
ACTTAGACAAAGAAATGTAG+TGG	-	chr8_4:18607840-18607859	None:intergenic	30.0%
TTTAATTGCCTGTAGAAAGT+AGG	-	chr8_4:18608016-18608035	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	18606611	18611051	18606611	ID=Msa1317380;Name=Msa1317380
chr8_4	mRNA	18606611	18611051	18606611	ID=Msa1317380-mRNA-1;Parent=Msa1317380;Name=Msa1317380-mRNA-1;_AED=0.08;_eAED=0.08;_QI=204\|0.85\|0.87\|1\|1\|1\|8\|373\|301
chr8_4	exon	18606611	18606919	18606611	ID=Msa1317380-mRNA-1:exon:6874;Parent=Msa1317380-mRNA-1
chr8_4	exon	18607124	18607234	18607124	ID=Msa1317380-mRNA-1:exon:6875;Parent=Msa1317380-mRNA-1
chr8_4	exon	18607322	18607420	18607322	ID=Msa1317380-mRNA-1:exon:6876;Parent=Msa1317380-mRNA-1
chr8_4	exon	18608027	18608128	18608027	ID=Msa1317380-mRNA-1:exon:6877;Parent=Msa1317380-mRNA-1
chr8_4	exon	18609359	18609525	18609359	ID=Msa1317380-mRNA-1:exon:6878;Parent=Msa1317380-mRNA-1
chr8_4	exon	18609989	18610067	18609989	ID=Msa1317380-mRNA-1:exon:6879;Parent=Msa1317380-mRNA-1
chr8_4	exon	18610146	18610316	18610146	ID=Msa1317380-mRNA-1:exon:6880;Parent=Msa1317380-mRNA-1
chr8_4	exon	18610607	18611051	18610607	ID=Msa1317380-mRNA-1:exon:6881;Parent=Msa1317380-mRNA-1
chr8_4	five_prime_UTR	18606611	18606814	18606611	ID=Msa1317380-mRNA-1:five_prime_utr;Parent=Msa1317380-mRNA-1
chr8_4	CDS	18606815	18606919	18606815	ID=Msa1317380-mRNA-1:cds;Parent=Msa1317380-mRNA-1
chr8_4	CDS	18607124	18607234	18607124	ID=Msa1317380-mRNA-1:cds;Parent=Msa1317380-mRNA-1
chr8_4	CDS	18607322	18607420	18607322	ID=Msa1317380-mRNA-1:cds;Parent=Msa1317380-mRNA-1
chr8_4	CDS	18608027	18608128	18608027	ID=Msa1317380-mRNA-1:cds;Parent=Msa1317380-mRNA-1
chr8_4	CDS	18609359	18609525	18609359	ID=Msa1317380-mRNA-1:cds;Parent=Msa1317380-mRNA-1
chr8_4	CDS	18609989	18610067	18609989	ID=Msa1317380-mRNA-1:cds;Parent=Msa1317380-mRNA-1
chr8_4	CDS	18610146	18610316	18610146	ID=Msa1317380-mRNA-1:cds;Parent=Msa1317380-mRNA-1
chr8_4	CDS	18610607	18610678	18610607	ID=Msa1317380-mRNA-1:cds;Parent=Msa1317380-mRNA-1
chr8_4	three_prime_UTR	18610679	18611051	18610679	ID=Msa1317380-mRNA-1:three_prime_utr;Parent=Msa1317380-mRNA-1

Gene Sequence

>Msa1317380

ATGGAGATTGGATTTCTGGGTTTGGGAATAATGGGCAAAGCCATGTCAATTAACCTTCTACGCCATGGCTTCAAAGTCACTGTTTGGAACAGAACCCTCTCTAAGTGTGATGAACTGGTGGAACATGGTGCTTCAGTTGGAGAAACACCTGCGGCTGTTGTCAAGAAGTGCAAGTATACAATTGCAATGTTATCCGATCCTGCAGCTGCTTTGGCGGTTGTGTTTGATAAGGATGGTGTTCTTGAGCAAATTAATGGAAAAGGTTATATTGACATGTCGACAGTCGATGCTGAGACATCTATCAAGATATCTGAGGCAATTAAAGCAAAAGGTGGTGACTTTCTTGAAGCTCCTGTTTCGGGTAGTAAGAAGCCTGCAGAAGATGGCCAACTAGTTATACTTGCTGCCGGAGACAAGGCATTCTATGATGAAGCACTTCCAGCATTTGATGTACTCGGGAAGAAATCTTTCTTTCTGGGTGAGGTTGGAAATGGAGCAAAAATGAAACTTGTTGTCAACATGGTAATGGGAAGGTACTTTTCATCCCCGTTTTCTCCTTCTGTTCATAATTTGGAAGTAGGAAGTATGATGAATGCTTTCTCTGAGGGACTGACACTTGCTGAAAGAAGTGGCTTGAACCCTAGCACACTTCTTGATGTGCTGGATCTTGGGGCCATAAGTAACGGCATGTTTAAATTGAAAGGACCAACAATGCTTAAAAACAGTTACGCCCCTGCTTTTCCACTGAAACATCAGCAGAAGGACATGAGACTGGCTCTTGCCCTTGGAGATGAAAATGCTGTACCAATGCCTGTCGCAGCTGCAGCAAATGAGGCATTCAAGAAAGCCAGAAGCATGGGGTTGGGAGACCTTGATTTTTCAGCTGTGCACGAGACTTTGAAATAA

Protein sequence

>Msa1317380

MEIGFLGLGIMGKAMSINLLRHGFKVTVWNRTLSKCDELVEHGASVGETPAAVVKKCKYTIAMLSDPAAALAVVFDKDGVLEQINGKGYIDMSTVDAETSIKISEAIKAKGGDFLEAPVSGSKKPAEDGQLVILAAGDKAFYDEALPAFDVLGKKSFFLGEVGNGAKMKLVVNMVMGRYFSSPFSPSVHNLEVGSMMNAFSEGLTLAERSGLNPSTLLDVLDLGAISNGMFKLKGPTMLKNSYAPAFPLKHQQKDMRLALALGDENAVPMPVAAAANEAFKKARSMGLGDLDFSAVHETLK*