AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1402930


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1402930 MtrunA17_Chr1g0198761 73.047 256 69 0 1 256 495 750 1.31e-135 399
Msa1402930 MtrunA17_Chr5g0430921 80.000 85 17 0 172 256 1 85 1.19e-44 145
Msa1402930 MtrunA17_Chr2g0290391 47.429 175 82 3 9 175 504 676 2.56e-42 152
Msa1402930 MtrunA17_Chr2g0294841 33.977 259 147 4 1 239 491 745 1.01e-40 149
Msa1402930 MtrunA17_Chr2g0276841 42.197 173 94 3 1 170 513 682 7.94e-39 143
Msa1402930 MtrunA17_Chr5g0440451 29.825 171 109 4 7 170 489 655 1.05e-15 76.6
Msa1402930 MtrunA17_Chr5g0448511 37.838 111 65 1 7 113 282 392 4.04e-15 74.7
Msa1402930 MtrunA17_Chr5g0448761 31.618 136 85 2 8 135 511 646 8.94e-14 70.9
Msa1402930 MtrunA17_Chr1g0202341 28.108 185 120 4 8 183 495 675 3.17e-13 68.9
Msa1402930 MtrunA17_Chr3g0118611 37.273 110 65 2 8 113 387 496 6.86e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1402930 AT5G28530.5 44.048 168 93 1 4 170 440 607 6.06e-41 149
Msa1402930 AT5G28530.4 44.048 168 93 1 4 170 444 611 6.52e-41 149
Msa1402930 AT5G28530.3 44.048 168 93 1 4 170 503 670 1.04e-40 148
Msa1402930 AT5G28530.2 44.048 168 93 1 4 170 503 670 1.04e-40 148
Msa1402930 AT5G28530.1 44.048 168 93 1 4 170 507 674 1.08e-40 148
Msa1402930 AT1G10240.2 43.103 174 91 3 1 170 491 660 1.19e-39 145
Msa1402930 AT1G10240.1 43.103 174 91 3 1 170 491 660 1.19e-39 145
Msa1402930 AT4G38170.1 37.607 117 69 2 7 119 284 400 5.51e-15 74.7
Msa1402930 AT2G27110.2 31.579 152 94 5 8 153 465 612 4.46e-12 66.2
Msa1402930 AT2G27110.1 31.579 152 94 5 8 153 465 612 4.46e-12 66.2
Msa1402930 AT4G38180.1 31.818 110 71 1 8 113 520 629 5.13e-12 65.9
Msa1402930 AT2G27110.3 31.579 152 94 5 8 153 320 467 7.07e-12 65.5

Find 39 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATCCAAACTTACTTATT+AGG 0.125684 tig0022949:+34590 Msa1402930:intergenic
TCATCACAAACATTGTTATT+TGG 0.171906 tig0022949:+34791 Msa1402930:intergenic
CAATCCAAACTTACTTATTA+GGG 0.237365 tig0022949:+34591 Msa1402930:intergenic
CTCATGTTGGAGCAACTTAA+AGG 0.285515 tig0022949:+35267 Msa1402930:intergenic
GTTGACTACATGGCTAGATT+TGG 0.300626 tig0022949:+34988 Msa1402930:intergenic
GCAGCTTCAACTCCTCCTTT+TGG 0.319527 tig0022949:+34719 Msa1402930:intergenic
TTGACTACATGGCTAGATTT+GGG 0.326040 tig0022949:+34989 Msa1402930:intergenic
TCAATCTCTGATTCGATGTT+TGG 0.328733 tig0022949:-34939 Msa1402930:CDS
TCAAGAATATGTGATTGATT+TGG 0.332734 tig0022949:-34825 Msa1402930:CDS
TGATCCTCTTGGCCGACCTT+TGG 0.339991 tig0022949:+34745 Msa1402930:intergenic
AAAGTCATTCAAGATATAAA+AGG 0.358979 tig0022949:-34860 Msa1402930:CDS
GATGCAGCATGTTCCTCAAT+AGG 0.398909 tig0022949:+35307 Msa1402930:intergenic
CTTTCTTGTCTCCATCGAAA+AGG 0.441125 tig0022949:+35022 Msa1402930:intergenic
GGAAAACATGTTCTGATATG+AGG 0.442250 tig0022949:+35328 Msa1402930:intergenic
ACATGTTATTCGTGTGCTAA+TGG 0.458320 tig0022949:-35080 Msa1402930:CDS
TTTCTTGTCTCCATCGAAAA+GGG 0.485154 tig0022949:+35023 Msa1402930:intergenic
TGATTAGTTCCACCACAACT+TGG 0.507884 tig0022949:+34671 Msa1402930:intergenic
CGCTGTCATGTTCCAAGTTG+TGG 0.511566 tig0022949:-34683 Msa1402930:CDS
CCAAGAGGATCAAGACCAAA+AGG 0.514192 tig0022949:-34734 Msa1402930:CDS
TGGAGCAACTTAAAGGCATA+TGG 0.514224 tig0022949:+35274 Msa1402930:intergenic
TAGATAACTCAATCTCATGT+TGG 0.522807 tig0022949:+35254 Msa1402930:intergenic
ATGGTGGGCGTCAAGTAAGT+TGG 0.528714 tig0022949:-35168 Msa1402930:CDS
TAGCATTGCAGTTGACTACA+TGG 0.529793 tig0022949:+34978 Msa1402930:intergenic
CAACATCATACAAAAGTTGA+TGG 0.542659 tig0022949:-35187 Msa1402930:CDS
ATCATACAAAAGTTGATGGT+GGG 0.568308 tig0022949:-35183 Msa1402930:CDS
TTGTTACAAAATCCAAAGGT+CGG 0.582656 tig0022949:-34757 Msa1402930:CDS
CCCATTGTTACAAAATCCAA+AGG 0.587534 tig0022949:-34761 Msa1402930:CDS
AATCCAAACTTACTTATTAG+GGG 0.596179 tig0022949:+34592 Msa1402930:intergenic
CAGAGATTGAAACTTAGTAG+AGG 0.604303 tig0022949:+34952 Msa1402930:intergenic
TGACTACATGGCTAGATTTG+GGG 0.607240 tig0022949:+34990 Msa1402930:intergenic
CATCATACAAAAGTTGATGG+TGG 0.610250 tig0022949:-35184 Msa1402930:CDS
TACTTATTAGGGGATTGAGA+AGG 0.610633 tig0022949:+34602 Msa1402930:intergenic
AAATCCAAAGGTCGGCCAAG+AGG 0.639624 tig0022949:-34749 Msa1402930:CDS
AGAGAACGAGTTGCTACTAG+AGG 0.645909 tig0022949:-34890 Msa1402930:CDS
GAGGAGTTGAAGCTGCAAAG+AGG 0.660327 tig0022949:-34712 Msa1402930:CDS
GCAGCAACTGAAATTGACAA+TGG 0.666988 tig0022949:-35223 Msa1402930:CDS
AGAGGATCAAGACCAAAAGG+AGG 0.673931 tig0022949:-34731 Msa1402930:CDS
TGTCATGTTCCAAGTTGTGG+TGG 0.684261 tig0022949:-34680 Msa1402930:CDS
CTTGGAACATGACAGCGACG+AGG 0.719993 tig0022949:+34689 Msa1402920:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATTTTTTTCTTTTTGTT+TGG + tig0022949:35321-35340 Msa1402930:intergenic 15.0%
!!! TCAATTTTTTTCTTTTTGTT+TGG + tig0022949:35321-35340 Msa1402930:intergenic 15.0%
!! AAAGTCATTCAAGATATAAA+AGG - tig0022949:35090-35109 Msa1402930:CDS 20.0%
!! AAAGTCATTCAAGATATAAA+AGG - tig0022949:35090-35109 Msa1402930:CDS 20.0%
!!! TGTAAAGAATTTGAGTTTTC+TGG - tig0022949:34835-34854 Msa1402930:CDS 25.0%
! TCAAGAATATGTGATTGATT+TGG - tig0022949:35125-35144 Msa1402930:CDS 25.0%
!! TCATCACAAACATTGTTATT+TGG + tig0022949:35162-35181 Msa1402930:intergenic 25.0%
!!! TGTAAAGAATTTGAGTTTTC+TGG - tig0022949:34835-34854 Msa1402930:CDS 25.0%
! TCAAGAATATGTGATTGATT+TGG - tig0022949:35125-35144 Msa1402930:CDS 25.0%
!! TCATCACAAACATTGTTATT+TGG + tig0022949:35162-35181 Msa1402930:intergenic 25.0%
TAGATAACTCAATCTCATGT+TGG + tig0022949:34699-34718 Msa1402930:intergenic 30.0%
CAACATCATACAAAAGTTGA+TGG - tig0022949:34763-34782 Msa1402930:CDS 30.0%
!! ATCATACAAAAGTTGATGGT+GGG - tig0022949:34767-34786 Msa1402930:CDS 30.0%
!! GAAAAGGGAGATATTTTGAA+GGG + tig0022949:34915-34934 Msa1402930:intergenic 30.0%
! CCTTTGGATTTTGTAACAAT+GGG + tig0022949:35192-35211 Msa1402930:intergenic 30.0%
! ACCTTTGGATTTTGTAACAA+TGG + tig0022949:35193-35212 Msa1402930:intergenic 30.0%
TTGTTACAAAATCCAAAGGT+CGG - tig0022949:35193-35212 Msa1402930:CDS 30.0%
TAGATAACTCAATCTCATGT+TGG + tig0022949:34699-34718 Msa1402930:intergenic 30.0%
CAACATCATACAAAAGTTGA+TGG - tig0022949:34763-34782 Msa1402930:CDS 30.0%
!! ATCATACAAAAGTTGATGGT+GGG - tig0022949:34767-34786 Msa1402930:CDS 30.0%
!! GAAAAGGGAGATATTTTGAA+GGG + tig0022949:34915-34934 Msa1402930:intergenic 30.0%
! CCTTTGGATTTTGTAACAAT+GGG + tig0022949:35192-35211 Msa1402930:intergenic 30.0%
! ACCTTTGGATTTTGTAACAA+TGG + tig0022949:35193-35212 Msa1402930:intergenic 30.0%
TTGTTACAAAATCCAAAGGT+CGG - tig0022949:35193-35212 Msa1402930:CDS 30.0%
GGAAAACATGTTCTGATATG+AGG + tig0022949:34625-34644 None:intergenic 35.0%
! CAGAACATGTTTTCCTATTG+AGG - tig0022949:34630-34649 Msa1402930:CDS 35.0%
!! CATCATACAAAAGTTGATGG+TGG - tig0022949:34766-34785 Msa1402930:CDS 35.0%
ACATGTTATTCGTGTGCTAA+TGG - tig0022949:34870-34889 Msa1402930:CDS 35.0%
!! CGAAAAGGGAGATATTTTGA+AGG + tig0022949:34916-34935 Msa1402930:intergenic 35.0%
! CAAAATATCTCCCTTTTCGA+TGG - tig0022949:34917-34936 Msa1402930:CDS 35.0%
TTTCTTGTCTCCATCGAAAA+GGG + tig0022949:34930-34949 Msa1402930:intergenic 35.0%
TTGACTACATGGCTAGATTT+GGG + tig0022949:34964-34983 Msa1402930:intergenic 35.0%
CAGAGATTGAAACTTAGTAG+AGG + tig0022949:35001-35020 Msa1402930:intergenic 35.0%
! TCAATCTCTGATTCGATGTT+TGG - tig0022949:35011-35030 Msa1402930:CDS 35.0%
CCCATTGTTACAAAATCCAA+AGG - tig0022949:35189-35208 Msa1402930:CDS 35.0%
TACTTATTAGGGGATTGAGA+AGG + tig0022949:35351-35370 Msa1402930:intergenic 35.0%
GGAAAACATGTTCTGATATG+AGG + tig0022949:34625-34644 Msa1402930:intergenic 35.0%
! CAGAACATGTTTTCCTATTG+AGG - tig0022949:34630-34649 Msa1402930:CDS 35.0%
!! CATCATACAAAAGTTGATGG+TGG - tig0022949:34766-34785 Msa1402930:CDS 35.0%
ACATGTTATTCGTGTGCTAA+TGG - tig0022949:34870-34889 Msa1402930:CDS 35.0%
!! CGAAAAGGGAGATATTTTGA+AGG + tig0022949:34916-34935 Msa1402930:intergenic 35.0%
! CAAAATATCTCCCTTTTCGA+TGG - tig0022949:34917-34936 Msa1402930:CDS 35.0%
TTTCTTGTCTCCATCGAAAA+GGG + tig0022949:34930-34949 Msa1402930:intergenic 35.0%
TTGACTACATGGCTAGATTT+GGG + tig0022949:34964-34983 Msa1402930:intergenic 35.0%
CAGAGATTGAAACTTAGTAG+AGG + tig0022949:35001-35020 Msa1402930:intergenic 35.0%
! TCAATCTCTGATTCGATGTT+TGG - tig0022949:35011-35030 Msa1402930:CDS 35.0%
CCCATTGTTACAAAATCCAA+AGG - tig0022949:35189-35208 Msa1402930:CDS 35.0%
TACTTATTAGGGGATTGAGA+AGG + tig0022949:35351-35370 Msa1402930:intergenic 35.0%
! TGGAGCAACTTAAAGGCATA+TGG + tig0022949:34679-34698 Msa1402930:intergenic 40.0%
CTCATGTTGGAGCAACTTAA+AGG + tig0022949:34686-34705 Msa1402930:intergenic 40.0%
GCAGCAACTGAAATTGACAA+TGG - tig0022949:34727-34746 Msa1402930:CDS 40.0%
!! AAGGGAGATATTTTGAAGGG+AGG + tig0022949:34912-34931 Msa1402930:intergenic 40.0%
CTTTCTTGTCTCCATCGAAA+AGG + tig0022949:34931-34950 Msa1402930:intergenic 40.0%
TGACTACATGGCTAGATTTG+GGG + tig0022949:34963-34982 Msa1402930:intergenic 40.0%
GTTGACTACATGGCTAGATT+TGG + tig0022949:34965-34984 Msa1402930:intergenic 40.0%
TAGCATTGCAGTTGACTACA+TGG + tig0022949:34975-34994 Msa1402930:intergenic 40.0%
! TGATTAGTTCCACCACAACT+TGG + tig0022949:35282-35301 Msa1402930:intergenic 40.0%
! TGGAGCAACTTAAAGGCATA+TGG + tig0022949:34679-34698 Msa1402930:intergenic 40.0%
CTCATGTTGGAGCAACTTAA+AGG + tig0022949:34686-34705 Msa1402930:intergenic 40.0%
GCAGCAACTGAAATTGACAA+TGG - tig0022949:34727-34746 Msa1402930:CDS 40.0%
!! AAGGGAGATATTTTGAAGGG+AGG + tig0022949:34912-34931 Msa1402930:intergenic 40.0%
CTTTCTTGTCTCCATCGAAA+AGG + tig0022949:34931-34950 Msa1402930:intergenic 40.0%
TGACTACATGGCTAGATTTG+GGG + tig0022949:34963-34982 Msa1402930:intergenic 40.0%
GTTGACTACATGGCTAGATT+TGG + tig0022949:34965-34984 Msa1402930:intergenic 40.0%
TAGCATTGCAGTTGACTACA+TGG + tig0022949:34975-34994 Msa1402930:intergenic 40.0%
! TGATTAGTTCCACCACAACT+TGG + tig0022949:35282-35301 Msa1402930:intergenic 40.0%
GATGCAGCATGTTCCTCAAT+AGG + tig0022949:34646-34665 Msa1402930:intergenic 45.0%
AGAGAACGAGTTGCTACTAG+AGG - tig0022949:35060-35079 Msa1402930:CDS 45.0%
!!! CCTTTTGGTCTTGATCCTCT+TGG + tig0022949:35219-35238 Msa1402930:intergenic 45.0%
CCAAGAGGATCAAGACCAAA+AGG - tig0022949:35216-35235 Msa1402930:CDS 45.0%
AGAGGATCAAGACCAAAAGG+AGG - tig0022949:35219-35238 Msa1402930:CDS 45.0%
TGTCATGTTCCAAGTTGTGG+TGG - tig0022949:35270-35289 Msa1402930:CDS 45.0%
GATGCAGCATGTTCCTCAAT+AGG + tig0022949:34646-34665 Msa1402930:intergenic 45.0%
AGAGAACGAGTTGCTACTAG+AGG - tig0022949:35060-35079 Msa1402930:CDS 45.0%
!!! CCTTTTGGTCTTGATCCTCT+TGG + tig0022949:35219-35238 Msa1402930:intergenic 45.0%
CCAAGAGGATCAAGACCAAA+AGG - tig0022949:35216-35235 Msa1402930:CDS 45.0%
AGAGGATCAAGACCAAAAGG+AGG - tig0022949:35219-35238 Msa1402930:CDS 45.0%
TGTCATGTTCCAAGTTGTGG+TGG - tig0022949:35270-35289 Msa1402930:CDS 45.0%
!! ATGGTGGGCGTCAAGTAAGT+TGG - tig0022949:34782-34801 Msa1402930:CDS 50.0%
AAATCCAAAGGTCGGCCAAG+AGG - tig0022949:35201-35220 Msa1402930:CDS 50.0%
! GCAGCTTCAACTCCTCCTTT+TGG + tig0022949:35234-35253 Msa1402930:intergenic 50.0%
GAGGAGTTGAAGCTGCAAAG+AGG - tig0022949:35238-35257 Msa1402930:CDS 50.0%
CGCTGTCATGTTCCAAGTTG+TGG - tig0022949:35267-35286 Msa1402930:CDS 50.0%
!! ATGGTGGGCGTCAAGTAAGT+TGG - tig0022949:34782-34801 Msa1402930:CDS 50.0%
AAATCCAAAGGTCGGCCAAG+AGG - tig0022949:35201-35220 Msa1402930:CDS 50.0%
! GCAGCTTCAACTCCTCCTTT+TGG + tig0022949:35234-35253 Msa1402930:intergenic 50.0%
GAGGAGTTGAAGCTGCAAAG+AGG - tig0022949:35238-35257 Msa1402930:CDS 50.0%
CGCTGTCATGTTCCAAGTTG+TGG - tig0022949:35267-35286 Msa1402930:CDS 50.0%
TGATCCTCTTGGCCGACCTT+TGG + tig0022949:35208-35227 Msa1402930:intergenic 55.0%
CTTGGAACATGACAGCGACG+AGG + tig0022949:35264-35283 Msa1402930:intergenic 55.0%
TGATCCTCTTGGCCGACCTT+TGG + tig0022949:35208-35227 Msa1402930:intergenic 55.0%
CTTGGAACATGACAGCGACG+AGG + tig0022949:35264-35283 Msa1402930:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0022949 gene 34601 35371 34601 ID=Msa1402930;Name=Msa1402930
tig0022949 mRNA 34601 35371 34601 ID=Msa1402930-mRNA-1;Parent=Msa1402930;Name=Msa1402930-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|256
tig0022949 exon 34601 35371 34601 ID=Msa1402930-mRNA-1:exon:19138;Parent=Msa1402930-mRNA-1
tig0022949 CDS 34601 35371 34601 ID=Msa1402930-mRNA-1:cds;Parent=Msa1402930-mRNA-1
Gene Sequence

>Msa1402930

ATGCGTCAAAAGTATCACAATCCTCATATCAGAACATGTTTTCCTATTGAGGAACATGCTGCATCTGTACTCACGCCATATGCCTTTAAGTTGCTCCAACATGAGATTGAGTTATCTACAAAATATGCAGCAACTGAAATTGACAATGGTTCTTATATTGTGCAACATCATACAAAAGTTGATGGTGGGCGTCAAGTAAGTTGGATAGTAGAAAACGAGTCAATTCATTGCTCTTGTAAAGAATTTGAGTTTTCTGGAATATTATGCAGACATGTTATTCGTGTGCTAATGGTGAAAAATTATTTTAGCCTCCCTTCAAAATATCTCCCTTTTCGATGGAGACAAGAAAGTTCATTGATCCCCAAATCTAGCCATGTAGTCAACTGCAATGCTACTTCCTCTACTAAGTTTCAATCTCTGATTCGATGTTTGGAAGTTGAATCATTAAAGACAAGCGAAAGAGAACGAGTTGCTACTAGAGGATTAGAAAAAGTCATTCAAGATATAAAAGGCATGCTAGAAACTCAAGAATATGTGATTGATTTGGAACATGATGCTCCAAATAACAATGTTTGTGATGATGAAAATCCCATTGTTACAAAATCCAAAGGTCGGCCAAGAGGATCAAGACCAAAAGGAGGAGTTGAAGCTGCAAAGAGGCCTCGTCGCTGTCATGTTCCAAGTTGTGGTGGAACTAATCATGACTCACGAAATTGTCCAAACAAAAAGAAAAAAATTGAAGTATTGCCTTCTCAATCCCCTAATAAGTAA

Protein sequence

>Msa1402930

MRQKYHNPHIRTCFPIEEHAASVLTPYAFKLLQHEIELSTKYAATEIDNGSYIVQHHTKVDGGRQVSWIVENESIHCSCKEFEFSGILCRHVIRVLMVKNYFSLPSKYLPFRWRQESSLIPKSSHVVNCNATSSTKFQSLIRCLEVESLKTSERERVATRGLEKVIQDIKGMLETQEYVIDLEHDAPNNNVCDDENPIVTKSKGRPRGSRPKGGVEAAKRPRRCHVPSCGGTNHDSRNCPNKKKKIEVLPSQSPNK*