AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0232850


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0232850 MtrunA17_Chr2g0308161 72.131 61 12 1 60 115 19 79 1.54e-15 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAACCATTTCTTGAAGTT+GGG 0.352117 2_2:+5111872 None:intergenic
TTTCAACCATTTCTTGAAGT+TGG 0.356872 2_2:+5111871 None:intergenic
CTAGTCCCAACTTCAAGAAA+TGG 0.372385 2_2:-5111877 Msa0232850:intron
AAGCAATTGGATGTAGAAAC+TGG 0.389575 2_2:-5112005 Msa0232850:CDS
ATGGGAAAGGAGTGTGTACC+AGG 0.471236 2_2:-5111813 Msa0232850:intron
CTTGAGGACAACATAGGTGA+TGG 0.475620 2_2:-5111389 Msa0232850:CDS
ACTTCAAGAAATGGTTGAAA+AGG 0.483448 2_2:-5111868 Msa0232850:CDS
AGGAAGTAGAGGAAAGCAAT+TGG 0.498254 2_2:-5112018 Msa0232850:CDS
ATGAAATTCAAGAGGATGAT+CGG 0.537624 2_2:-5111316 Msa0232850:CDS
GAATTTCTTGAGGACAACAT+AGG 0.539954 2_2:-5111395 Msa0232850:CDS
TCAAGAAATGGTTGAAAAGG+AGG 0.541538 2_2:-5111865 Msa0232850:CDS
TTGAGGACAACATAGGTGAT+GGG 0.545907 2_2:-5111388 Msa0232850:CDS
CGCATATCATGAATTTCTTG+AGG 0.553600 2_2:-5111405 Msa0232850:CDS
GAAACTCGACAAGGAAGTAG+AGG 0.581711 2_2:-5112029 Msa0232850:CDS
TATATATGATGAATTTCAAG+AGG 0.613332 2_2:-5111351 Msa0232850:CDS
TCTAGATGATGAAATTCAAG+AGG 0.613572 2_2:-5111324 Msa0232850:CDS
CATAGGTGATGGGTTTCAGA+AGG 0.615780 2_2:-5111378 Msa0232850:CDS
CATAGATGAGGAATTTCAAG+AGG 0.630357 2_2:-5111432 Msa0232850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATGTAAAAATTTAAAGTTA+CGG - chr2_2:5111380-5111399 Msa0232850:CDS 10.0%
!!! TATGTAAAAATTTAAAGTTA+CGG - chr2_2:5111380-5111399 Msa0232850:CDS 10.0%
!! AAATTGTGTTTAACTAATAT+AGG - chr2_2:5111404-5111423 Msa0232850:CDS 15.0%
!! AAATTGTGTTTAACTAATAT+AGG - chr2_2:5111404-5111423 Msa0232850:CDS 15.0%
!! AAGTTGAAGAAACTTAAAAA+TGG - chr2_2:5111633-5111652 Msa0232850:intron 20.0%
!! AGTTGAAGAAACTTAAAAAT+GGG - chr2_2:5111634-5111653 Msa0232850:intron 20.0%
!!! TTTTATTTTCATATGCAACA+AGG - chr2_2:5111827-5111846 Msa0232850:CDS 20.0%
!!! AATTATTTTCATATGCAACA+AGG - chr2_2:5111869-5111888 Msa0232850:CDS 20.0%
!! TATATATGATGAATTTCAAG+AGG - chr2_2:5112000-5112019 Msa0232850:intron 20.0%
!! AAGTTGAAGAAACTTAAAAA+TGG - chr2_2:5111633-5111652 Msa0232850:intron 20.0%
!! AGTTGAAGAAACTTAAAAAT+GGG - chr2_2:5111634-5111653 Msa0232850:intron 20.0%
!!! TTTTATTTTCATATGCAACA+AGG - chr2_2:5111827-5111846 Msa0232850:CDS 20.0%
!!! AATTATTTTCATATGCAACA+AGG - chr2_2:5111869-5111888 Msa0232850:CDS 20.0%
!! TATATATGATGAATTTCAAG+AGG - chr2_2:5112000-5112019 Msa0232850:intron 20.0%
! TTGTGTTTAACTAATATAGG+CGG - chr2_2:5111407-5111426 Msa0232850:CDS 25.0%
! TTGTAATGATAGAATTAGGA+AGG - chr2_2:5111658-5111677 Msa0232850:intron 25.0%
! TGTAATGATAGAATTAGGAA+GGG - chr2_2:5111659-5111678 Msa0232850:intron 25.0%
! CTTTGAATCAACAATATCTA+CGG - chr2_2:5111791-5111810 Msa0232850:intron 25.0%
! TTGTGTTTAACTAATATAGG+CGG - chr2_2:5111407-5111426 Msa0232850:CDS 25.0%
! TTGTAATGATAGAATTAGGA+AGG - chr2_2:5111658-5111677 Msa0232850:intron 25.0%
! TGTAATGATAGAATTAGGAA+GGG - chr2_2:5111659-5111678 Msa0232850:intron 25.0%
! CTTTGAATCAACAATATCTA+CGG - chr2_2:5111791-5111810 Msa0232850:intron 25.0%
!!! ATGTTTTTTGAAACTCGACA+AGG - chr2_2:5111313-5111332 Msa0232850:CDS 30.0%
TTCAACCATTTCTTGAAGTT+GGG + chr2_2:5111482-5111501 None:intergenic 30.0%
TTTCAACCATTTCTTGAAGT+TGG + chr2_2:5111483-5111502 None:intergenic 30.0%
ACTTCAAGAAATGGTTGAAA+AGG - chr2_2:5111483-5111502 Msa0232850:CDS 30.0%
!!! GAAGCTTTTAAAGCTGTTAT+GGG - chr2_2:5111520-5111539 Msa0232850:intron 30.0%
!!! TTTTAAAGCTGTTATGGGAA+AGG - chr2_2:5111525-5111544 Msa0232850:intron 30.0%
CAAAACAAATGTTCTACTTG+CGG - chr2_2:5111689-5111708 Msa0232850:intron 30.0%
!!! ATTTGGTTTTTATTTGTGCG+AGG - chr2_2:5111740-5111759 Msa0232850:intron 30.0%
!! CGTAGATATTGTTGATTCAA+AGG + chr2_2:5111793-5111812 None:intergenic 30.0%
TGAAAAAGATGACATAGATG+AGG - chr2_2:5111907-5111926 Msa0232850:intron 30.0%
TCTAGATGATGAAATTCAAG+AGG - chr2_2:5112027-5112046 Msa0232850:CDS 30.0%
ATGAAATTCAAGAGGATGAT+CGG - chr2_2:5112035-5112054 Msa0232850:CDS 30.0%
!!! ATGTTTTTTGAAACTCGACA+AGG - chr2_2:5111313-5111332 Msa0232850:CDS 30.0%
TTCAACCATTTCTTGAAGTT+GGG + chr2_2:5111482-5111501 None:intergenic 30.0%
TTTCAACCATTTCTTGAAGT+TGG + chr2_2:5111483-5111502 None:intergenic 30.0%
ACTTCAAGAAATGGTTGAAA+AGG - chr2_2:5111483-5111502 Msa0232850:CDS 30.0%
!!! GAAGCTTTTAAAGCTGTTAT+GGG - chr2_2:5111520-5111539 Msa0232850:intron 30.0%
!!! TTTTAAAGCTGTTATGGGAA+AGG - chr2_2:5111525-5111544 Msa0232850:intron 30.0%
CAAAACAAATGTTCTACTTG+CGG - chr2_2:5111689-5111708 Msa0232850:intron 30.0%
!!! ATTTGGTTTTTATTTGTGCG+AGG - chr2_2:5111740-5111759 Msa0232850:intron 30.0%
!! CGTAGATATTGTTGATTCAA+AGG + chr2_2:5111793-5111812 None:intergenic 30.0%
TGAAAAAGATGACATAGATG+AGG - chr2_2:5111907-5111926 Msa0232850:intron 30.0%
TCTAGATGATGAAATTCAAG+AGG - chr2_2:5112027-5112046 Msa0232850:CDS 30.0%
ATGAAATTCAAGAGGATGAT+CGG - chr2_2:5112035-5112054 Msa0232850:CDS 30.0%
AAGCAATTGGATGTAGAAAC+TGG - chr2_2:5111346-5111365 Msa0232850:CDS 35.0%
TCAAGAAATGGTTGAAAAGG+AGG - chr2_2:5111486-5111505 Msa0232850:CDS 35.0%
!!! CGAAGCTTTTAAAGCTGTTA+TGG - chr2_2:5111519-5111538 Msa0232850:intron 35.0%
GGGCTTGTAATGATAGAATT+AGG - chr2_2:5111654-5111673 Msa0232850:intron 35.0%
!! GCGTGTTTTACTTGTTGATT+TGG - chr2_2:5111723-5111742 Msa0232850:intron 35.0%
CATAGATGAGGAATTTCAAG+AGG - chr2_2:5111919-5111938 Msa0232850:intron 35.0%
CGCATATCATGAATTTCTTG+AGG - chr2_2:5111946-5111965 Msa0232850:intron 35.0%
GAATTTCTTGAGGACAACAT+AGG - chr2_2:5111956-5111975 Msa0232850:intron 35.0%
AAGCAATTGGATGTAGAAAC+TGG - chr2_2:5111346-5111365 Msa0232850:CDS 35.0%
TCAAGAAATGGTTGAAAAGG+AGG - chr2_2:5111486-5111505 Msa0232850:CDS 35.0%
!!! CGAAGCTTTTAAAGCTGTTA+TGG - chr2_2:5111519-5111538 Msa0232850:intron 35.0%
GGGCTTGTAATGATAGAATT+AGG - chr2_2:5111654-5111673 Msa0232850:intron 35.0%
!! GCGTGTTTTACTTGTTGATT+TGG - chr2_2:5111723-5111742 Msa0232850:intron 35.0%
CATAGATGAGGAATTTCAAG+AGG - chr2_2:5111919-5111938 Msa0232850:intron 35.0%
CGCATATCATGAATTTCTTG+AGG - chr2_2:5111946-5111965 Msa0232850:intron 35.0%
GAATTTCTTGAGGACAACAT+AGG - chr2_2:5111956-5111975 Msa0232850:intron 35.0%
AGGAAGTAGAGGAAAGCAAT+TGG - chr2_2:5111333-5111352 Msa0232850:CDS 40.0%
CTAGTCCCAACTTCAAGAAA+TGG - chr2_2:5111474-5111493 Msa0232850:CDS 40.0%
AACACAATCAAAATCCGACC+TGG + chr2_2:5111559-5111578 None:intergenic 40.0%
TTGAGGACAACATAGGTGAT+GGG - chr2_2:5111963-5111982 Msa0232850:intron 40.0%
AGGAAGTAGAGGAAAGCAAT+TGG - chr2_2:5111333-5111352 Msa0232850:CDS 40.0%
CTAGTCCCAACTTCAAGAAA+TGG - chr2_2:5111474-5111493 Msa0232850:CDS 40.0%
AACACAATCAAAATCCGACC+TGG + chr2_2:5111559-5111578 None:intergenic 40.0%
TTGAGGACAACATAGGTGAT+GGG - chr2_2:5111963-5111982 Msa0232850:intron 40.0%
GAAACTCGACAAGGAAGTAG+AGG - chr2_2:5111322-5111341 Msa0232850:CDS 45.0%
CTTGAGGACAACATAGGTGA+TGG - chr2_2:5111962-5111981 Msa0232850:intron 45.0%
!! CATAGGTGATGGGTTTCAGA+AGG - chr2_2:5111973-5111992 Msa0232850:intron 45.0%
GAAACTCGACAAGGAAGTAG+AGG - chr2_2:5111322-5111341 Msa0232850:CDS 45.0%
CTTGAGGACAACATAGGTGA+TGG - chr2_2:5111962-5111981 Msa0232850:intron 45.0%
!! CATAGGTGATGGGTTTCAGA+AGG - chr2_2:5111973-5111992 Msa0232850:intron 45.0%
! ATGGGAAAGGAGTGTGTACC+AGG - chr2_2:5111538-5111557 Msa0232850:intron 50.0%
! GAAAGGAGTGTGTACCAGGT+CGG - chr2_2:5111542-5111561 Msa0232850:intron 50.0%
! ATGGGAAAGGAGTGTGTACC+AGG - chr2_2:5111538-5111557 Msa0232850:intron 50.0%
! GAAAGGAGTGTGTACCAGGT+CGG - chr2_2:5111542-5111561 Msa0232850:intron 50.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 5111313 5112060 5111313 ID=Msa0232850;Name=Msa0232850
chr2_2 mRNA 5111313 5112060 5111313 ID=Msa0232850-mRNA-1;Parent=Msa0232850;Name=Msa0232850-mRNA-1;_AED=0.37;_eAED=0.38;_QI=0|0|0|1|1|1|3|0|117
chr2_2 exon 5112001 5112060 5112001 ID=Msa0232850-mRNA-1:exon:2255;Parent=Msa0232850-mRNA-1
chr2_2 exon 5111814 5111895 5111814 ID=Msa0232850-mRNA-1:exon:2254;Parent=Msa0232850-mRNA-1
chr2_2 exon 5111313 5111524 5111313 ID=Msa0232850-mRNA-1:exon:2253;Parent=Msa0232850-mRNA-1
chr2_2 CDS 5112001 5112060 5112001 ID=Msa0232850-mRNA-1:cds;Parent=Msa0232850-mRNA-1
chr2_2 CDS 5111814 5111895 5111814 ID=Msa0232850-mRNA-1:cds;Parent=Msa0232850-mRNA-1
chr2_2 CDS 5111313 5111524 5111313 ID=Msa0232850-mRNA-1:cds;Parent=Msa0232850-mRNA-1
Gene Sequence

>Msa0232850

ATGTTTTTTGAAACTCGACAAGGAAGTAGAGGAAAGCAATTGGATGTAGAAACTGGAAAATCCCAACTTCAAGAAATGGTTGAAAAGGAGGAAAGTGATATCGAAGCTTTTAAAGCTGTTATGGGAAAGGAGTGTGTACCAGGTATCGATGAAGATGTTGAAAATTATTTTCATATGCAACAAGGTATCGATGAAGATGTTGAAAAAGATGACATAGATGAGGAATTTCAAGAGGAAGACGCATATCATGAATTTCTTGAGGACAACATAGGTGATGGGTTTCAGAAGGATGATATATATGATGAATTTCAAGAGGATGATCTAGATGATGAAATTCAAGAGGATGATCGGTGA

Protein sequence

>Msa0232850

MFFETRQGSRGKQLDVETGKSQLQEMVEKEESDIEAFKAVMGKECVPGIDEDVENYFHMQQGIDEDVEKDDIDEEFQEEDAYHEFLEDNIGDGFQKDDIYDEFQEDDLDDEIQEDDR*