Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1432800 | KEH37197.1 | 95.652 | 69 | 3 | 0 | 1 | 69 | 1 | 69 | 1.84e-39 | 134 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1432800 | A0A072V5T5 | 95.652 | 69 | 3 | 0 | 1 | 69 | 1 | 69 | 8.79e-40 | 134 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1432790 | Msa1432800 | 0.956387 | 3.325079e-114 | -8.615850e-47 |
| Msa1432800 | Msa1450020 | 0.814713 | 1.339467e-51 | -8.615850e-47 |
| Msa0628100 | Msa1432800 | 0.801509 | 8.709253e-49 | -8.615850e-47 |
| Msa0643020 | Msa1432800 | -0.808108 | 3.641244e-50 | -8.615850e-47 |
| Msa0807050 | Msa1432800 | 0.804096 | 2.545497e-49 | -8.615850e-47 |
| Msa0832990 | Msa1432800 | 0.805176 | 1.514398e-49 | -8.615850e-47 |
| Msa0200370 | Msa1432800 | 0.954263 | 4.384233e-112 | -8.615850e-47 |
| Msa0418430 | Msa1432800 | 0.805005 | 1.644549e-49 | -8.615850e-47 |
| Msa0419620 | Msa1432800 | -0.841890 | 3.611158e-58 | -8.615850e-47 |
| Msa0243470 | Msa1432800 | 0.809144 | 2.187432e-50 | -8.615850e-47 |
| Msa0281270 | Msa1432800 | 0.933460 | 1.814383e-95 | -8.615850e-47 |
| Msa0281940 | Msa1432800 | 0.816821 | 4.539257e-52 | -8.615850e-47 |
| Msa0282170 | Msa1432800 | 0.815154 | 1.069169e-51 | -8.615850e-47 |
| Msa0309550 | Msa1432800 | 0.829876 | 4.026852e-55 | -8.615850e-47 |
| Msa0320440 | Msa1432800 | 0.955128 | 6.175160e-113 | -8.615850e-47 |
| Msa1132950 | Msa1432800 | 0.812456 | 4.201261e-51 | -8.615850e-47 |
| Msa0511720 | Msa1432800 | -0.817514 | 3.169478e-52 | -8.615850e-47 |
| Msa0601920 | Msa1432800 | -0.803419 | 3.517072e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1432800 | MtrunA17_Chr2g0295501 | 96.078 | 51 | 2 | 0 | 1 | 51 | 1 | 51 | 2.69e-31 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCATGCAAGATCGTGCTTC+TGG | 0.277502 | tig0027178:-24106 | Msa1432800:CDS |
| CTAAGGAAGATTGGCTTAGA+AGG | 0.290742 | tig0027178:-24172 | Msa1432800:CDS |
| TGCATGCTGACCCTTCTTGT+TGG | 0.323679 | tig0027178:+24121 | None:intergenic |
| GCATGCAAGATCGTGCTTCT+GGG | 0.397696 | tig0027178:-24105 | Msa1432800:CDS |
| TCATCAGACCTAAGGAAGAT+TGG | 0.412607 | tig0027178:-24181 | Msa1432800:CDS |
| CTTCTAAGCCAATCTTCCTT+AGG | 0.445559 | tig0027178:+24173 | None:intergenic |
| GCTTCTGGGGTTAGCAAGGA+TGG | 0.500998 | tig0027178:-24091 | Msa1432800:CDS |
| TAAGGAAGATTGGCTTAGAA+GGG | 0.540665 | tig0027178:-24171 | Msa1432800:CDS |
| CACCTCCTTCATCAGACCTA+AGG | 0.563117 | tig0027178:-24189 | Msa1432800:CDS |
| CATGCAAGATCGTGCTTCTG+GGG | 0.577242 | tig0027178:-24104 | Msa1432800:CDS |
| TCGTGCTTCTGGGGTTAGCA+AGG | 0.589442 | tig0027178:-24095 | Msa1432800:CDS |
| ATCTTCCTTAGGTCTGATGA+AGG | 0.601958 | tig0027178:+24184 | None:intergenic |
| TTCCTTAGGTCTGATGAAGG+AGG | 0.672818 | tig0027178:+24187 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAGTGCTTAAAATTTAAA+GGG | + | tig0027178:24065-24084 | None:intergenic | 20.0% |
| !! | AAGTAGTGCTTAAAATTTAA+AGG | + | tig0027178:24066-24085 | None:intergenic | 20.0% |
| !! | GCTTTAATAGACAAGTTTTA+TGG | - | tig0027178:23918-23937 | Msa1432800:CDS | 25.0% |
| !! | ACTTTGCCTTAAATTTTAAG+TGG | - | tig0027178:24082-24101 | Msa1432800:CDS | 25.0% |
| !!! | CTTTGCCTTAAATTTTAAGT+GGG | - | tig0027178:24083-24102 | Msa1432800:CDS | 25.0% |
| TGAGACCCACTTAAAATTTA+AGG | + | tig0027178:24091-24110 | None:intergenic | 30.0% | |
| CAGTTTCATCTTTCATGTTA+TGG | - | tig0027178:24109-24128 | Msa1432800:CDS | 30.0% | |
| ATATGTAGAAAAAAGCTCGA+AGG | - | tig0027178:24139-24158 | Msa1432800:CDS | 30.0% | |
| !! | TAAGGAAGATTGGCTTAGAA+GGG | - | tig0027178:23892-23911 | Msa1432800:CDS | 35.0% |
| !! | AGTTTTATGGTCCAACAAGA+AGG | - | tig0027178:23931-23950 | Msa1432800:intron | 35.0% |
| ! | GTTTTATGGTCCAACAAGAA+GGG | - | tig0027178:23932-23951 | Msa1432800:intron | 35.0% |
| ATCGAATGTAAGTTACCCAA+AGG | - | tig0027178:24016-24035 | Msa1432800:intron | 35.0% | |
| TCGAATGTAAGTTACCCAAA+GGG | - | tig0027178:24017-24036 | Msa1432800:intron | 35.0% | |
| ATCTTCCTTAGGTCTGATGA+AGG | + | tig0027178:23882-23901 | None:intergenic | 40.0% | |
| TCATCAGACCTAAGGAAGAT+TGG | - | tig0027178:23882-23901 | Msa1432800:CDS | 40.0% | |
| CTTCTAAGCCAATCTTCCTT+AGG | + | tig0027178:23893-23912 | None:intergenic | 40.0% | |
| !! | CTAAGGAAGATTGGCTTAGA+AGG | - | tig0027178:23891-23910 | Msa1432800:CDS | 40.0% |
| CGAATGTAAGTTACCCAAAG+GGG | - | tig0027178:24018-24037 | Msa1432800:intron | 40.0% | |
| TTCCTTAGGTCTGATGAAGG+AGG | + | tig0027178:23879-23898 | None:intergenic | 45.0% | |
| !! | AACGATTTTGGGTCCCCTTT+GGG | + | tig0027178:24034-24053 | None:intergenic | 45.0% |
| AAAGGGGACCCAAAATCGTT+GGG | - | tig0027178:24034-24053 | Msa1432800:intron | 45.0% | |
| CACCTCCTTCATCAGACCTA+AGG | - | tig0027178:23874-23893 | Msa1432800:CDS | 50.0% | |
| TGCATGCTGACCCTTCTTGT+TGG | + | tig0027178:23945-23964 | None:intergenic | 50.0% | |
| AGCATGCAAGATCGTGCTTC+TGG | - | tig0027178:23957-23976 | Msa1432800:intron | 50.0% | |
| GCATGCAAGATCGTGCTTCT+GGG | - | tig0027178:23958-23977 | Msa1432800:intron | 50.0% | |
| CATGCAAGATCGTGCTTCTG+GGG | - | tig0027178:23959-23978 | Msa1432800:intron | 50.0% | |
| !! | CAACGATTTTGGGTCCCCTT+TGG | + | tig0027178:24035-24054 | None:intergenic | 50.0% |
| CAAAGGGGACCCAAAATCGT+TGG | - | tig0027178:24033-24052 | Msa1432800:intron | 50.0% | |
| AAGGGGACCCAAAATCGTTG+GGG | - | tig0027178:24035-24054 | Msa1432800:intron | 50.0% | |
| ! | GGGTCTACCCCAACGATTTT+GGG | + | tig0027178:24045-24064 | None:intergenic | 50.0% |
| ! | AGGGTCTACCCCAACGATTT+TGG | + | tig0027178:24046-24065 | None:intergenic | 50.0% |
| !! | TCGTGCTTCTGGGGTTAGCA+AGG | - | tig0027178:23968-23987 | Msa1432800:intron | 55.0% |
| !! | GCTTCTGGGGTTAGCAAGGA+TGG | - | tig0027178:23972-23991 | Msa1432800:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0027178 | gene | 23870 | 24215 | 23870 | ID=Msa1432800;Name=Msa1432800 |
| tig0027178 | mRNA | 23870 | 24215 | 23870 | ID=Msa1432800-mRNA-1;Parent=Msa1432800;Name=Msa1432800-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|73 |
| tig0027178 | exon | 24063 | 24215 | 24063 | ID=Msa1432800-mRNA-1:exon:8320;Parent=Msa1432800-mRNA-1 |
| tig0027178 | exon | 23870 | 23938 | 23870 | ID=Msa1432800-mRNA-1:exon:8319;Parent=Msa1432800-mRNA-1 |
| tig0027178 | CDS | 24063 | 24215 | 24063 | ID=Msa1432800-mRNA-1:cds;Parent=Msa1432800-mRNA-1 |
| tig0027178 | CDS | 23870 | 23938 | 23870 | ID=Msa1432800-mRNA-1:cds;Parent=Msa1432800-mRNA-1 |
| Gene Sequence |
| Protein sequence |
