Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0281270 | KEH37197.1 | 80.000 | 65 | 13 | 0 | 1 | 65 | 1 | 65 | 3.48e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0281270 | A0A072V5T5 | 80.000 | 65 | 13 | 0 | 1 | 65 | 1 | 65 | 1.66e-29 | 108 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0198440 | Msa0281270 | 0.811302 | 7.494679e-51 | -8.615850e-47 |
| Msa0200370 | Msa0281270 | 0.963782 | 1.652286e-122 | -8.615850e-47 |
| Msa0200870 | Msa0281270 | 0.800053 | 1.726348e-48 | -8.615850e-47 |
| Msa0243470 | Msa0281270 | 0.820690 | 6.003287e-53 | -8.615850e-47 |
| Msa0278670 | Msa0281270 | 0.813744 | 2.192147e-51 | -8.615850e-47 |
| Msa0281270 | Msa0281940 | 0.826718 | 2.325741e-54 | -8.615850e-47 |
| Msa0281270 | Msa0282170 | 0.837591 | 4.749434e-57 | -8.615850e-47 |
| Msa0281270 | Msa0309550 | 0.864811 | 9.297311e-65 | -8.615850e-47 |
| Msa0281270 | Msa0318070 | 0.814471 | 1.514860e-51 | -8.615850e-47 |
| Msa0281270 | Msa0320440 | 0.978690 | 2.352181e-146 | -8.615850e-47 |
| Msa0281270 | Msa0363460 | 0.803324 | 3.681238e-49 | -8.615850e-47 |
| Msa0281270 | Msa0418430 | 0.808542 | 2.942736e-50 | -8.615850e-47 |
| Msa0281270 | Msa0419620 | -0.852496 | 4.445156e-61 | -8.615850e-47 |
| Msa0281270 | Msa0431830 | 0.803633 | 3.175714e-49 | -8.615850e-47 |
| Msa0281270 | Msa0455230 | 0.803605 | 3.218450e-49 | -8.615850e-47 |
| Msa0281270 | Msa0462590 | 0.800244 | 1.578407e-48 | -8.615850e-47 |
| Msa0281270 | Msa0511720 | -0.839700 | 1.354392e-57 | -8.615850e-47 |
| Msa0281270 | Msa0530950 | 0.817884 | 2.615986e-52 | -8.615850e-47 |
| Msa0281270 | Msa0576600 | 0.803350 | 3.635692e-49 | -8.615850e-47 |
| Msa0281270 | Msa0593810 | 0.800703 | 1.272552e-48 | -8.615850e-47 |
| Msa0281270 | Msa0628100 | 0.815685 | 8.144439e-52 | -8.615850e-47 |
| Msa0281270 | Msa0656270 | 0.806018 | 1.008195e-49 | -8.615850e-47 |
| Msa0281270 | Msa0700070 | 0.807473 | 4.968006e-50 | -8.615850e-47 |
| Msa0281270 | Msa0714340 | 0.804559 | 2.037925e-49 | -8.615850e-47 |
| Msa0281270 | Msa0714600 | 0.801784 | 7.647289e-49 | -8.615850e-47 |
| Msa0281270 | Msa0722930 | 0.826888 | 2.118100e-54 | -8.615850e-47 |
| Msa0281270 | Msa0723140 | 0.816994 | 4.149496e-52 | -8.615850e-47 |
| Msa0281270 | Msa0756310 | 0.800076 | 1.707778e-48 | -8.615850e-47 |
| Msa0281270 | Msa0801360 | 0.813156 | 2.951886e-51 | -8.615850e-47 |
| Msa0281270 | Msa0807050 | 0.824203 | 9.165001e-54 | -8.615850e-47 |
| Msa0281270 | Msa0832990 | 0.824440 | 8.063641e-54 | -8.615850e-47 |
| Msa0281270 | Msa0839830 | 0.808401 | 3.153697e-50 | -8.615850e-47 |
| Msa0281270 | Msa0961420 | -0.800130 | 1.664846e-48 | -8.615850e-47 |
| Msa0281270 | Msa1070850 | 0.809216 | 2.110990e-50 | -8.615850e-47 |
| Msa0281270 | Msa1132950 | 0.835911 | 1.273605e-56 | -8.615850e-47 |
| Msa0281270 | Msa1187520 | 0.805139 | 1.542031e-49 | -8.615850e-47 |
| Msa0281270 | Msa1217810 | 0.814639 | 1.390597e-51 | -8.615850e-47 |
| Msa0281270 | Msa1282920 | 0.821803 | 3.325007e-53 | -8.615850e-47 |
| Msa0281270 | Msa1292730 | 0.809606 | 1.741647e-50 | -8.615850e-47 |
| Msa0281270 | Msa1421870 | 0.801654 | 8.130357e-49 | -8.615850e-47 |
| Msa0281270 | Msa1427830 | 0.805630 | 1.216396e-49 | -8.615850e-47 |
| Msa0281270 | Msa1432790 | 0.978869 | 9.759643e-147 | -8.615850e-47 |
| Msa0281270 | Msa1432800 | 0.933460 | 1.814383e-95 | -8.615850e-47 |
| Msa0281270 | Msa1450020 | 0.831163 | 1.950423e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0281270 | MtrunA17_Chr2g0295501 | 89.286 | 56 | 6 | 0 | 1 | 56 | 1 | 56 | 2.45e-31 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCATGCAAGATCGTGCTTC+TGG | 0.277502 | 2_3:-16634749 | Msa0281270:CDS |
| CTAAGGAAGATTGGCTTAGA+AGG | 0.290742 | 2_3:-16634815 | Msa0281270:CDS |
| TGCATGCTGACCCTTCTTGT+TGG | 0.323679 | 2_3:+16634764 | None:intergenic |
| GCATGCAAGATCGTGCTTCT+GGG | 0.397696 | 2_3:-16634748 | Msa0281270:CDS |
| TCATCAGACCTAAGGAAGAT+TGG | 0.412607 | 2_3:-16634824 | Msa0281270:CDS |
| CTTCTAAGCCAATCTTCCTT+AGG | 0.445559 | 2_3:+16634816 | None:intergenic |
| GCTTCTGGGGTTAGCAAGGA+TGG | 0.500998 | 2_3:-16634734 | Msa0281270:CDS |
| TAAGGAAGATTGGCTTAGAA+GGG | 0.540665 | 2_3:-16634814 | Msa0281270:CDS |
| CACCTCCTTCATCAGACCTA+AGG | 0.563117 | 2_3:-16634832 | Msa0281270:CDS |
| CATGCAAGATCGTGCTTCTG+GGG | 0.577242 | 2_3:-16634747 | Msa0281270:CDS |
| TCGTGCTTCTGGGGTTAGCA+AGG | 0.589442 | 2_3:-16634738 | Msa0281270:CDS |
| ATCTTCCTTAGGTCTGATGA+AGG | 0.601958 | 2_3:+16634827 | None:intergenic |
| TTCCTTAGGTCTGATGAAGG+AGG | 0.672818 | 2_3:+16634830 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAGTGCTTAAAATTTAAA+GGG | + | chr2_3:16634733-16634752 | None:intergenic | 20.0% |
| !! | AAGTAGTGCTTAAAATTTAA+AGG | + | chr2_3:16634734-16634753 | None:intergenic | 20.0% |
| !! | GCTTTAATAGACAAGTTTTA+TGG | - | chr2_3:16634586-16634605 | Msa0281270:intron | 25.0% |
| !! | ACTTTGCCTTAAATTTTAAG+TGG | - | chr2_3:16634750-16634769 | Msa0281270:CDS | 25.0% |
| !!! | CTTTGCCTTAAATTTTAAGT+GGG | - | chr2_3:16634751-16634770 | Msa0281270:CDS | 25.0% |
| TGAGACCCACTTAAAATTTA+AGG | + | chr2_3:16634759-16634778 | None:intergenic | 30.0% | |
| TATGTAGAAAAAAAGCTCGA+AGG | - | chr2_3:16634801-16634820 | Msa0281270:CDS | 30.0% | |
| !! | TAAGGAAGATTGGCTTAGAA+GGG | - | chr2_3:16634560-16634579 | Msa0281270:CDS | 35.0% |
| !! | AGTTTTATGGTCCAACAAGA+AGG | - | chr2_3:16634599-16634618 | Msa0281270:intron | 35.0% |
| ! | GTTTTATGGTCCAACAAGAA+GGG | - | chr2_3:16634600-16634619 | Msa0281270:intron | 35.0% |
| ATCGAATGTAAGTTACCCAA+AGG | - | chr2_3:16634684-16634703 | Msa0281270:intron | 35.0% | |
| TCGAATGTAAGTTACCCAAA+GGG | - | chr2_3:16634685-16634704 | Msa0281270:intron | 35.0% | |
| ATCTTCCTTAGGTCTGATGA+AGG | + | chr2_3:16634550-16634569 | None:intergenic | 40.0% | |
| TCATCAGACCTAAGGAAGAT+TGG | - | chr2_3:16634550-16634569 | Msa0281270:CDS | 40.0% | |
| CTTCTAAGCCAATCTTCCTT+AGG | + | chr2_3:16634561-16634580 | None:intergenic | 40.0% | |
| !! | CTAAGGAAGATTGGCTTAGA+AGG | - | chr2_3:16634559-16634578 | Msa0281270:CDS | 40.0% |
| CGAATGTAAGTTACCCAAAG+GGG | - | chr2_3:16634686-16634705 | Msa0281270:intron | 40.0% | |
| TGGGTCTCAGTTTCATGTTA+TGG | - | chr2_3:16634770-16634789 | Msa0281270:CDS | 40.0% | |
| TTCCTTAGGTCTGATGAAGG+AGG | + | chr2_3:16634547-16634566 | None:intergenic | 45.0% | |
| !! | AACGATTTTGGGTCCCCTTT+GGG | + | chr2_3:16634702-16634721 | None:intergenic | 45.0% |
| AAAGGGGACCCAAAATCGTT+GGG | - | chr2_3:16634702-16634721 | Msa0281270:intron | 45.0% | |
| CACCTCCTTCATCAGACCTA+AGG | - | chr2_3:16634542-16634561 | Msa0281270:CDS | 50.0% | |
| TGCATGCTGACCCTTCTTGT+TGG | + | chr2_3:16634613-16634632 | None:intergenic | 50.0% | |
| AGCATGCAAGATCGTGCTTC+TGG | - | chr2_3:16634625-16634644 | Msa0281270:intron | 50.0% | |
| GCATGCAAGATCGTGCTTCT+GGG | - | chr2_3:16634626-16634645 | Msa0281270:intron | 50.0% | |
| CATGCAAGATCGTGCTTCTG+GGG | - | chr2_3:16634627-16634646 | Msa0281270:intron | 50.0% | |
| !! | CAACGATTTTGGGTCCCCTT+TGG | + | chr2_3:16634703-16634722 | None:intergenic | 50.0% |
| CAAAGGGGACCCAAAATCGT+TGG | - | chr2_3:16634701-16634720 | Msa0281270:intron | 50.0% | |
| AAGGGGACCCAAAATCGTTG+GGG | - | chr2_3:16634703-16634722 | Msa0281270:intron | 50.0% | |
| ! | GGGTCTACCCCAACGATTTT+GGG | + | chr2_3:16634713-16634732 | None:intergenic | 50.0% |
| ! | AGGGTCTACCCCAACGATTT+TGG | + | chr2_3:16634714-16634733 | None:intergenic | 50.0% |
| !! | TCGTGCTTCTGGGGTTAGCA+AGG | - | chr2_3:16634636-16634655 | Msa0281270:intron | 55.0% |
| !! | GCTTCTGGGGTTAGCAAGGA+TGG | - | chr2_3:16634640-16634659 | Msa0281270:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 16634538 | 16634858 | 16634538 | ID=Msa0281270;Name=Msa0281270 |
| chr2_3 | mRNA | 16634538 | 16634858 | 16634538 | ID=Msa0281270-mRNA-1;Parent=Msa0281270;Name=Msa0281270-mRNA-1;_AED=0.32;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|67 |
| chr2_3 | exon | 16634706 | 16634858 | 16634706 | ID=Msa0281270-mRNA-1:exon:6845;Parent=Msa0281270-mRNA-1 |
| chr2_3 | exon | 16634538 | 16634588 | 16634538 | ID=Msa0281270-mRNA-1:exon:6844;Parent=Msa0281270-mRNA-1 |
| chr2_3 | CDS | 16634706 | 16634858 | 16634706 | ID=Msa0281270-mRNA-1:cds;Parent=Msa0281270-mRNA-1 |
| chr2_3 | CDS | 16634538 | 16634588 | 16634538 | ID=Msa0281270-mRNA-1:cds;Parent=Msa0281270-mRNA-1 |
| Gene Sequence |
| Protein sequence |
