Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1432790 | KEH37197.1 | 80.000 | 65 | 13 | 0 | 1 | 65 | 1 | 65 | 3.48e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1432790 | A0A072V5T5 | 80.000 | 65 | 13 | 0 | 1 | 65 | 1 | 65 | 1.66e-29 | 108 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1432790 | Msa1432800 | 0.956387 | 3.325079e-114 | -8.615850e-47 |
| Msa1432790 | Msa1450020 | 0.823358 | 1.446155e-53 | -8.615850e-47 |
| Msa0628100 | Msa1432790 | 0.813846 | 2.081961e-51 | -8.615850e-47 |
| Msa0722930 | Msa1432790 | 0.814771 | 1.299910e-51 | -8.615850e-47 |
| Msa0723140 | Msa1432790 | 0.804838 | 1.782535e-49 | -8.615850e-47 |
| Msa0801360 | Msa1432790 | 0.804824 | 1.794146e-49 | -8.615850e-47 |
| Msa0807050 | Msa1432790 | 0.816215 | 6.203156e-52 | -8.615850e-47 |
| Msa0832990 | Msa1432790 | 0.816981 | 4.177791e-52 | -8.615850e-47 |
| Msa0839830 | Msa1432790 | 0.800105 | 1.684344e-48 | -8.615850e-47 |
| Msa0200370 | Msa1432790 | 0.973085 | 7.781401e-136 | -8.615850e-47 |
| Msa0200870 | Msa1432790 | 0.802190 | 6.311302e-49 | -8.615850e-47 |
| Msa1282920 | Msa1432790 | 0.811314 | 7.447289e-51 | -8.615850e-47 |
| Msa1292730 | Msa1432790 | 0.800518 | 1.387927e-48 | -8.615850e-47 |
| Msa0418430 | Msa1432790 | 0.807714 | 4.415692e-50 | -8.615850e-47 |
| Msa0419620 | Msa1432790 | -0.846443 | 2.164769e-59 | -8.615850e-47 |
| Msa0431830 | Msa1432790 | 0.803721 | 3.044571e-49 | -8.615850e-47 |
| Msa0243470 | Msa1432790 | 0.819575 | 1.080870e-52 | -8.615850e-47 |
| Msa0278670 | Msa1432790 | 0.801048 | 1.082233e-48 | -8.615850e-47 |
| Msa0281270 | Msa1432790 | 0.978869 | 9.759643e-147 | -8.615850e-47 |
| Msa0281940 | Msa1432790 | 0.833540 | 5.029416e-56 | -8.615850e-47 |
| Msa0282170 | Msa1432790 | 0.829226 | 5.793868e-55 | -8.615850e-47 |
| Msa0309550 | Msa1432790 | 0.844070 | 9.494142e-59 | -8.615850e-47 |
| Msa0318070 | Msa1432790 | 0.803402 | 3.546682e-49 | -8.615850e-47 |
| Msa0320440 | Msa1432790 | 0.988352 | 1.109971e-173 | -8.615850e-47 |
| Msa1132950 | Msa1432790 | 0.823717 | 1.191806e-53 | -8.615850e-47 |
| Msa0511720 | Msa1432790 | -0.829950 | 3.863617e-55 | -8.615850e-47 |
| Msa0530950 | Msa1432790 | 0.811611 | 6.419289e-51 | -8.615850e-47 |
| Msa0576600 | Msa1432790 | 0.801917 | 7.180530e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1432790 | MtrunA17_Chr2g0295501 | 89.286 | 56 | 6 | 0 | 1 | 56 | 1 | 56 | 2.45e-31 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCATGCAAGATCGTGCTTC+TGG | 0.277502 | tig0027178:-7786 | Msa1432790:CDS |
| CTAAGGAAGATTGGCTTAGA+AGG | 0.290742 | tig0027178:-7852 | Msa1432790:CDS |
| TGCATGCTGACCCTTCTTGT+TGG | 0.323679 | tig0027178:+7801 | None:intergenic |
| GCATGCAAGATCGTGCTTCT+GGG | 0.397696 | tig0027178:-7785 | Msa1432790:CDS |
| TCATCAGACCTAAGGAAGAT+TGG | 0.412607 | tig0027178:-7861 | Msa1432790:CDS |
| CTTCTAAGCCAATCTTCCTT+AGG | 0.445559 | tig0027178:+7853 | None:intergenic |
| GCTTCTGGGGTTAGCAAGGA+TGG | 0.500998 | tig0027178:-7771 | Msa1432790:CDS |
| TAAGGAAGATTGGCTTAGAA+GGG | 0.540665 | tig0027178:-7851 | Msa1432790:CDS |
| CACCTCCTTCATCAGACCTA+AGG | 0.563117 | tig0027178:-7869 | Msa1432790:CDS |
| CATGCAAGATCGTGCTTCTG+GGG | 0.577242 | tig0027178:-7784 | Msa1432790:CDS |
| TCGTGCTTCTGGGGTTAGCA+AGG | 0.589442 | tig0027178:-7775 | Msa1432790:CDS |
| ATCTTCCTTAGGTCTGATGA+AGG | 0.601958 | tig0027178:+7864 | None:intergenic |
| TTCCTTAGGTCTGATGAAGG+AGG | 0.672818 | tig0027178:+7867 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAGTGCTTAAAATTTAAA+GGG | + | tig0027178:7770-7789 | None:intergenic | 20.0% |
| !! | AAGTAGTGCTTAAAATTTAA+AGG | + | tig0027178:7771-7790 | None:intergenic | 20.0% |
| !! | GCTTTAATAGACAAGTTTTA+TGG | - | tig0027178:7623-7642 | Msa1432790:intron | 25.0% |
| !! | ACTTTGCCTTAAATTTTAAG+TGG | - | tig0027178:7787-7806 | Msa1432790:CDS | 25.0% |
| !!! | CTTTGCCTTAAATTTTAAGT+GGG | - | tig0027178:7788-7807 | Msa1432790:CDS | 25.0% |
| TGAGACCCACTTAAAATTTA+AGG | + | tig0027178:7796-7815 | None:intergenic | 30.0% | |
| TATGTAGAAAAAAAGCTCGA+AGG | - | tig0027178:7838-7857 | Msa1432790:CDS | 30.0% | |
| !! | TAAGGAAGATTGGCTTAGAA+GGG | - | tig0027178:7597-7616 | Msa1432790:CDS | 35.0% |
| !! | AGTTTTATGGTCCAACAAGA+AGG | - | tig0027178:7636-7655 | Msa1432790:intron | 35.0% |
| ! | GTTTTATGGTCCAACAAGAA+GGG | - | tig0027178:7637-7656 | Msa1432790:intron | 35.0% |
| ATCGAATGTAAGTTACCCAA+AGG | - | tig0027178:7721-7740 | Msa1432790:intron | 35.0% | |
| TCGAATGTAAGTTACCCAAA+GGG | - | tig0027178:7722-7741 | Msa1432790:intron | 35.0% | |
| ATCTTCCTTAGGTCTGATGA+AGG | + | tig0027178:7587-7606 | None:intergenic | 40.0% | |
| TCATCAGACCTAAGGAAGAT+TGG | - | tig0027178:7587-7606 | Msa1432790:CDS | 40.0% | |
| CTTCTAAGCCAATCTTCCTT+AGG | + | tig0027178:7598-7617 | None:intergenic | 40.0% | |
| !! | CTAAGGAAGATTGGCTTAGA+AGG | - | tig0027178:7596-7615 | Msa1432790:CDS | 40.0% |
| CGAATGTAAGTTACCCAAAG+GGG | - | tig0027178:7723-7742 | Msa1432790:intron | 40.0% | |
| TGGGTCTCAGTTTCATGTTA+TGG | - | tig0027178:7807-7826 | Msa1432790:CDS | 40.0% | |
| TTCCTTAGGTCTGATGAAGG+AGG | + | tig0027178:7584-7603 | None:intergenic | 45.0% | |
| !! | AACGATTTTGGGTCCCCTTT+GGG | + | tig0027178:7739-7758 | None:intergenic | 45.0% |
| AAAGGGGACCCAAAATCGTT+GGG | - | tig0027178:7739-7758 | Msa1432790:intron | 45.0% | |
| CACCTCCTTCATCAGACCTA+AGG | - | tig0027178:7579-7598 | Msa1432790:CDS | 50.0% | |
| TGCATGCTGACCCTTCTTGT+TGG | + | tig0027178:7650-7669 | None:intergenic | 50.0% | |
| AGCATGCAAGATCGTGCTTC+TGG | - | tig0027178:7662-7681 | Msa1432790:intron | 50.0% | |
| GCATGCAAGATCGTGCTTCT+GGG | - | tig0027178:7663-7682 | Msa1432790:intron | 50.0% | |
| CATGCAAGATCGTGCTTCTG+GGG | - | tig0027178:7664-7683 | Msa1432790:intron | 50.0% | |
| !! | CAACGATTTTGGGTCCCCTT+TGG | + | tig0027178:7740-7759 | None:intergenic | 50.0% |
| CAAAGGGGACCCAAAATCGT+TGG | - | tig0027178:7738-7757 | Msa1432790:intron | 50.0% | |
| AAGGGGACCCAAAATCGTTG+GGG | - | tig0027178:7740-7759 | Msa1432790:intron | 50.0% | |
| ! | GGGTCTACCCCAACGATTTT+GGG | + | tig0027178:7750-7769 | None:intergenic | 50.0% |
| ! | AGGGTCTACCCCAACGATTT+TGG | + | tig0027178:7751-7770 | None:intergenic | 50.0% |
| !! | TCGTGCTTCTGGGGTTAGCA+AGG | - | tig0027178:7673-7692 | Msa1432790:intron | 55.0% |
| !! | GCTTCTGGGGTTAGCAAGGA+TGG | - | tig0027178:7677-7696 | Msa1432790:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0027178 | gene | 7575 | 7895 | 7575 | ID=Msa1432790;Name=Msa1432790 |
| tig0027178 | mRNA | 7575 | 7895 | 7575 | ID=Msa1432790-mRNA-1;Parent=Msa1432790;Name=Msa1432790-mRNA-1;_AED=0.10;_eAED=0.32;_QI=0|0|0|1|1|1|2|0|67 |
| tig0027178 | exon | 7743 | 7895 | 7743 | ID=Msa1432790-mRNA-1:exon:8322;Parent=Msa1432790-mRNA-1 |
| tig0027178 | exon | 7575 | 7625 | 7575 | ID=Msa1432790-mRNA-1:exon:8321;Parent=Msa1432790-mRNA-1 |
| tig0027178 | CDS | 7743 | 7895 | 7743 | ID=Msa1432790-mRNA-1:cds;Parent=Msa1432790-mRNA-1 |
| tig0027178 | CDS | 7575 | 7625 | 7575 | ID=Msa1432790-mRNA-1:cds;Parent=Msa1432790-mRNA-1 |
| Gene Sequence |
| Protein sequence |
