Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1460720 | XP_003610667.1 | 95.122 | 164 | 8 | 0 | 1 | 164 | 38 | 201 | 3.27e-109 | 319 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1460720 | sp|Q84MC0|UGPI4_ARATH | 60.123 | 163 | 62 | 2 | 2 | 164 | 39 | 198 | 8.86e-65 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1460720 | B7FGN4 | 95.122 | 164 | 8 | 0 | 1 | 164 | 38 | 201 | 1.56e-109 | 319 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0062340 | Msa1460720 | 0.805696 | 1.178643e-49 | -8.615850e-47 |
| Msa0077560 | Msa1460720 | 0.805689 | 1.182618e-49 | -8.615850e-47 |
| Msa1371910 | Msa1460720 | 0.844727 | 6.320625e-59 | -8.615850e-47 |
| Msa1450190 | Msa1460720 | 0.811852 | 5.689113e-51 | -8.615850e-47 |
| Msa1451820 | Msa1460720 | 0.801495 | 8.765965e-49 | -8.615850e-47 |
| Msa0607800 | Msa1460720 | 0.809504 | 1.831724e-50 | -8.615850e-47 |
| Msa0648040 | Msa1460720 | 0.802313 | 5.952588e-49 | -8.615850e-47 |
| Msa0711400 | Msa1460720 | 0.893032 | 9.164729e-75 | -8.615850e-47 |
| Msa0716890 | Msa1460720 | 0.809675 | 1.682841e-50 | -8.615850e-47 |
| Msa0751870 | Msa1460720 | 0.853653 | 2.072298e-61 | -8.615850e-47 |
| Msa0754430 | Msa1460720 | 0.813572 | 2.391146e-51 | -8.615850e-47 |
| Msa0780910 | Msa1460720 | 0.805781 | 1.130944e-49 | -8.615850e-47 |
| Msa0821160 | Msa1460720 | 0.812320 | 4.498727e-51 | -8.615850e-47 |
| Msa1279150 | Msa1460720 | 0.800588 | 1.343224e-48 | -8.615850e-47 |
| Msa0383000 | Msa1460720 | 0.809463 | 1.869329e-50 | -8.615850e-47 |
| Msa0421130 | Msa1460720 | 0.802360 | 5.820338e-49 | -8.615850e-47 |
| Msa0466880 | Msa1460720 | 0.816060 | 6.718411e-52 | -8.615850e-47 |
| Msa1031410 | Msa1460720 | 0.806111 | 9.639357e-50 | -8.615850e-47 |
| Msa1035610 | Msa1460720 | 0.809880 | 1.520530e-50 | -8.615850e-47 |
| Msa1132530 | Msa1460720 | 0.811949 | 5.419121e-51 | -8.615850e-47 |
| Msa1201780 | Msa1460720 | 0.804367 | 2.235140e-49 | -8.615850e-47 |
| Msa0519760 | Msa1460720 | 0.808934 | 2.425847e-50 | -8.615850e-47 |
| Msa0519810 | Msa1460720 | 0.805098 | 1.572636e-49 | -8.615850e-47 |
| Msa0519840 | Msa1460720 | 0.806666 | 7.362613e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1460720 | MtrunA17_Chr5g0394151 | 95.122 | 164 | 8 | 0 | 1 | 164 | 38 | 201 | 3.00e-113 | 319 |
| Msa1460720 | MtrunA17_Chr1g0205951 | 69.325 | 163 | 44 | 1 | 2 | 164 | 43 | 199 | 2.00e-80 | 236 |
| Msa1460720 | MtrunA17_Chr1g0191761 | 41.406 | 128 | 73 | 2 | 2 | 127 | 50 | 177 | 3.18e-31 | 111 |
| Msa1460720 | MtrunA17_Chr7g0272941 | 41.481 | 135 | 76 | 2 | 2 | 133 | 40 | 174 | 5.42e-31 | 110 |
| Msa1460720 | MtrunA17_Chr1g0191781 | 35.172 | 145 | 92 | 2 | 2 | 144 | 42 | 186 | 5.34e-27 | 100 |
| Msa1460720 | MtrunA17_Chr1g0191771 | 35.484 | 124 | 79 | 1 | 1 | 123 | 57 | 180 | 1.57e-23 | 92.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1460720 | AT3G06035.1 | 60.123 | 163 | 62 | 2 | 2 | 164 | 39 | 198 | 9.01e-66 | 199 |
| Msa1460720 | AT5G19250.1 | 66.667 | 132 | 44 | 0 | 2 | 133 | 40 | 171 | 1.14e-61 | 189 |
| Msa1460720 | AT1G54860.1 | 42.138 | 159 | 89 | 3 | 2 | 157 | 42 | 200 | 2.84e-43 | 142 |
| Msa1460720 | AT5G19240.1 | 47.500 | 160 | 70 | 4 | 1 | 157 | 39 | 187 | 4.07e-41 | 137 |
| Msa1460720 | AT5G19230.2 | 52.381 | 126 | 51 | 2 | 2 | 127 | 40 | 156 | 1.34e-36 | 125 |
| Msa1460720 | AT5G19230.1 | 52.381 | 126 | 51 | 2 | 2 | 127 | 40 | 156 | 1.34e-36 | 125 |
Find 39 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGCTTTATCACCAAAAT+TGG | 0.148873 | tig0041063:+546 | Msa1460720:CDS |
| GACTCAAAGTTTACTGGAAT+TGG | 0.202771 | tig0041063:+420 | Msa1460720:CDS |
| GTAATGCCTGCTTGTGTTCC+TGG | 0.222223 | tig0041063:+336 | Msa1460720:CDS |
| GGATTGGTTCCGAAGATAAT+TGG | 0.229248 | tig0041063:+442 | Msa1460720:CDS |
| CTTAATGACTCAAAGTTTAC+TGG | 0.244932 | tig0041063:+414 | Msa1460720:CDS |
| TTAAGTGACATTTAGTTAAA+AGG | 0.252789 | tig0041063:-287 | None:intergenic |
| GCAGTAAACCAATCCAATTT+TGG | 0.309912 | tig0041063:-559 | None:intergenic |
| TGAGGAAGATGATCTTTATC+AGG | 0.311325 | tig0041063:+95 | Msa1460720:five_prime_UTR |
| TTGTGTTCCTGGCCTTGTTC+CGG | 0.361906 | tig0041063:+347 | Msa1460720:CDS |
| AGTTAAAAGGTCTTCATAGT+TGG | 0.372121 | tig0041063:-274 | None:intergenic |
| ACTCAAAGTTTACTGGAATT+GGG | 0.389579 | tig0041063:+421 | Msa1460720:CDS |
| TACAAGGTTGATTCTTGAAC+TGG | 0.391876 | tig0041063:-203 | None:intergenic |
| GTTAAAAGGTCTTCATAGTT+GGG | 0.403819 | tig0041063:-273 | None:intergenic |
| TGCTCATTGCTCTTGTTAGT+TGG | 0.425769 | tig0041063:+579 | Msa1460720:CDS |
| CTTTATCACCAAAATTGGAT+TGG | 0.444400 | tig0041063:+551 | Msa1460720:CDS |
| AACAACAATCCAATTATCTT+CGG | 0.461927 | tig0041063:-451 | None:intergenic |
| CTCGTCATCACTAGAACTCT+CGG | 0.472274 | tig0041063:-511 | None:intergenic |
| AGTGATGACGAGACTTCTAA+TGG | 0.482206 | tig0041063:+522 | Msa1460720:CDS |
| AAGTTTACTGGAATTGGGAT+TGG | 0.486609 | tig0041063:+426 | Msa1460720:CDS |
| ACAATGTCCGGAACAAGGCC+AGG | 0.505794 | tig0041063:-354 | None:intergenic |
| TCTTCATAGTTGGGTAATTG+AGG | 0.505804 | tig0041063:-264 | None:intergenic |
| TACAGATCCATCCCTTGTGT+TGG | 0.505945 | tig0041063:-316 | None:intergenic |
| AATATTTCCAACACAAGGGA+TGG | 0.513314 | tig0041063:+309 | Msa1460720:CDS |
| GCACCAGTGGTATTTGTACA+AGG | 0.517231 | tig0041063:-219 | None:intergenic |
| ACAAGGCCAGGAACACAAGC+AGG | 0.520245 | tig0041063:-342 | None:intergenic |
| AAATTAGAGAGGACAATGTC+CGG | 0.542734 | tig0041063:-366 | None:intergenic |
| GGTAATTGAGGCTCAGTACC+TGG | 0.547704 | tig0041063:-252 | None:intergenic |
| CAACCTTGTACAAATACCAC+TGG | 0.568045 | tig0041063:+216 | Msa1460720:CDS |
| ACTTAAATATTTCCAACACA+AGG | 0.568858 | tig0041063:+304 | Msa1460720:CDS |
| TGGTATTGTGTTAGCACCAG+TGG | 0.589333 | tig0041063:-232 | None:intergenic |
| TCGTCATCACTAGAACTCTC+GGG | 0.593940 | tig0041063:-510 | None:intergenic |
| GAGGAAGATGATCTTTATCA+GGG | 0.594123 | tig0041063:+96 | Msa1460720:five_prime_UTR |
| GAGATTTCGTGAAATTAGAG+AGG | 0.595369 | tig0041063:-377 | None:intergenic |
| AGAGGACAATGTCCGGAACA+AGG | 0.617052 | tig0041063:-359 | None:intergenic |
| ACTGGTGCTAACACAATACC+AGG | 0.625811 | tig0041063:+234 | Msa1460720:CDS |
| CTTAAATATTTCCAACACAA+GGG | 0.640170 | tig0041063:+305 | Msa1460720:CDS |
| GCTTCCGTCAGAAGTGCTTG+TGG | 0.642715 | tig0041063:-481 | None:intergenic |
| TTGACCACAAGCACTTCTGA+CGG | 0.644420 | tig0041063:+477 | Msa1460720:CDS |
| ATGTCAAGTTCAATGATGTG+CGG | 0.702075 | tig0041063:-131 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAGTGACATTTAGTTAAA+AGG | - | tig0041063:290-309 | None:intergenic | 20.0% |
| ! | ACTTAAATATTTCCAACACA+AGG | + | tig0041063:304-323 | Msa1460720:CDS | 25.0% |
| ! | CTTAAATATTTCCAACACAA+GGG | + | tig0041063:305-324 | Msa1460720:CDS | 25.0% |
| ! | AACAACAATCCAATTATCTT+CGG | - | tig0041063:454-473 | None:intergenic | 25.0% |
| GTTAAAAGGTCTTCATAGTT+GGG | - | tig0041063:276-295 | None:intergenic | 30.0% | |
| AGTTAAAAGGTCTTCATAGT+TGG | - | tig0041063:277-296 | None:intergenic | 30.0% | |
| CTTAATGACTCAAAGTTTAC+TGG | + | tig0041063:414-433 | Msa1460720:CDS | 30.0% | |
| ACTCAAAGTTTACTGGAATT+GGG | + | tig0041063:421-440 | Msa1460720:CDS | 30.0% | |
| ! | GAAAGCTTTATCACCAAAAT+TGG | + | tig0041063:546-565 | Msa1460720:CDS | 30.0% |
| CTTTATCACCAAAATTGGAT+TGG | + | tig0041063:551-570 | Msa1460720:CDS | 30.0% | |
| TGAGGAAGATGATCTTTATC+AGG | + | tig0041063:95-114 | Msa1460720:five_prime_UTR | 35.0% | |
| GAGGAAGATGATCTTTATCA+GGG | + | tig0041063:96-115 | Msa1460720:five_prime_UTR | 35.0% | |
| ATGTCAAGTTCAATGATGTG+CGG | - | tig0041063:134-153 | None:intergenic | 35.0% | |
| ! | TACAAGGTTGATTCTTGAAC+TGG | - | tig0041063:206-225 | None:intergenic | 35.0% |
| TCTTCATAGTTGGGTAATTG+AGG | - | tig0041063:267-286 | None:intergenic | 35.0% | |
| AATATTTCCAACACAAGGGA+TGG | + | tig0041063:309-328 | Msa1460720:CDS | 35.0% | |
| AAATTAGAGAGGACAATGTC+CGG | - | tig0041063:369-388 | None:intergenic | 35.0% | |
| GAGATTTCGTGAAATTAGAG+AGG | - | tig0041063:380-399 | None:intergenic | 35.0% | |
| GACTCAAAGTTTACTGGAAT+TGG | + | tig0041063:420-439 | Msa1460720:CDS | 35.0% | |
| AAGTTTACTGGAATTGGGAT+TGG | + | tig0041063:426-445 | Msa1460720:CDS | 35.0% | |
| ! | GCAGTAAACCAATCCAATTT+TGG | - | tig0041063:562-581 | None:intergenic | 35.0% |
| CAACCTTGTACAAATACCAC+TGG | + | tig0041063:216-235 | Msa1460720:CDS | 40.0% | |
| !! | GGATTGGTTCCGAAGATAAT+TGG | + | tig0041063:442-461 | Msa1460720:CDS | 40.0% |
| ! | AGTGATGACGAGACTTCTAA+TGG | + | tig0041063:522-541 | Msa1460720:CDS | 40.0% |
| TGCTCATTGCTCTTGTTAGT+TGG | + | tig0041063:579-598 | Msa1460720:CDS | 40.0% | |
| GCACCAGTGGTATTTGTACA+AGG | - | tig0041063:222-241 | None:intergenic | 45.0% | |
| TGGTATTGTGTTAGCACCAG+TGG | - | tig0041063:235-254 | None:intergenic | 45.0% | |
| ! | ACTGGTGCTAACACAATACC+AGG | + | tig0041063:234-253 | Msa1460720:CDS | 45.0% |
| TACAGATCCATCCCTTGTGT+TGG | - | tig0041063:319-338 | None:intergenic | 45.0% | |
| TTGACCACAAGCACTTCTGA+CGG | + | tig0041063:477-496 | Msa1460720:CDS | 45.0% | |
| TCGTCATCACTAGAACTCTC+GGG | - | tig0041063:513-532 | None:intergenic | 45.0% | |
| CTCGTCATCACTAGAACTCT+CGG | - | tig0041063:514-533 | None:intergenic | 45.0% | |
| GGTAATTGAGGCTCAGTACC+TGG | - | tig0041063:255-274 | None:intergenic | 50.0% | |
| GTAATGCCTGCTTGTGTTCC+TGG | + | tig0041063:336-355 | Msa1460720:CDS | 50.0% | |
| !! | TTGTGTTCCTGGCCTTGTTC+CGG | + | tig0041063:347-366 | Msa1460720:CDS | 50.0% |
| AGAGGACAATGTCCGGAACA+AGG | - | tig0041063:362-381 | None:intergenic | 50.0% | |
| ACAAGGCCAGGAACACAAGC+AGG | - | tig0041063:345-364 | None:intergenic | 55.0% | |
| ACAATGTCCGGAACAAGGCC+AGG | - | tig0041063:357-376 | None:intergenic | 55.0% | |
| !! | GCTTCCGTCAGAAGTGCTTG+TGG | - | tig0041063:484-503 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0041063 | gene | 94 | 617 | 94 | ID=Msa1460720;Name=Msa1460720 |
| tig0041063 | mRNA | 94 | 617 | 94 | ID=Msa1460720-mRNA-1;Parent=Msa1460720;Name=Msa1460720-mRNA-1;_AED=0.01;_eAED=0.07;_QI=32|-1|0|1|-1|1|1|0|164 |
| tig0041063 | exon | 94 | 617 | 94 | ID=Msa1460720-mRNA-1:exon:22873;Parent=Msa1460720-mRNA-1 |
| tig0041063 | five_prime_UTR | 94 | 125 | 94 | ID=Msa1460720-mRNA-1:five_prime_utr;Parent=Msa1460720-mRNA-1 |
| tig0041063 | CDS | 126 | 617 | 126 | ID=Msa1460720-mRNA-1:cds;Parent=Msa1460720-mRNA-1 |
| Gene Sequence |
| Protein sequence |
