AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0751870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0751870 MtrunA17_Chr5g0394151 94.527 201 11 0 1 201 1 201 2.51e-141 392
Msa0751870 MtrunA17_Chr1g0205951 65.366 205 61 3 1 201 1 199 1.67e-94 273
Msa0751870 MtrunA17_Chr7g0272941 40.000 160 92 3 14 170 16 174 1.15e-35 124
Msa0751870 MtrunA17_Chr1g0191761 40.645 155 90 2 12 164 23 177 3.64e-35 123
Msa0751870 MtrunA17_Chr1g0191781 33.702 181 118 2 3 181 6 186 8.87e-32 114
Msa0751870 MtrunA17_Chr1g0191771 36.296 135 85 1 27 160 46 180 2.26e-27 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0751870 AT3G06035.1 58.791 182 72 2 20 201 20 198 5.38e-71 214
Msa0751870 AT5G19250.1 65.972 144 49 0 27 170 28 171 1.48e-66 203
Msa0751870 AT1G54860.1 41.237 194 109 4 5 193 6 199 6.42e-51 163
Msa0751870 AT5G19240.1 46.784 171 77 4 27 194 28 187 7.08e-43 143
Msa0751870 AT5G19230.2 48.485 165 75 3 1 164 1 156 7.22e-42 140
Msa0751870 AT5G19230.1 48.485 165 75 3 1 164 1 156 7.22e-42 140

Find 65 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCTTTATCACCAAAAT+TGG 0.148873 5_2:+5435560 Msa0751870:CDS
TAAACTAAACTAAACTTATT+TGG 0.179548 5_2:+5434043 Msa0751870:five_prime_UTR
GACTCAAAGTTTACTGGAAT+TGG 0.202771 5_2:+5435434 Msa0751870:CDS
GTAATGCCTGCTTGTGTTCC+TGG 0.222223 5_2:+5435350 Msa0751870:CDS
GGATTGGTTCCGAAGATAAT+TGG 0.229248 5_2:+5435456 Msa0751870:CDS
CTTAATGACTCAAAGTTTAC+TGG 0.244932 5_2:+5435428 Msa0751870:CDS
TTAAGTGACATTTAGTTAAA+AGG 0.252789 5_2:-5435301 None:intergenic
GCAGTAAACCAATCCAATTT+TGG 0.309912 5_2:-5435573 None:intergenic
TGAGGAAGATGATCTTTATC+AGG 0.311325 5_2:+5435109 Msa0751870:CDS
ACAATAAATTGTTCTATTGA+TGG 0.334563 5_2:+5435951 Msa0751870:three_prime_UTR
TTCCTTCTATTCTTCTTCTC+TGG 0.339502 5_2:-5435828 None:intergenic
TTGTGTTCCTGGCCTTGTTC+CGG 0.361906 5_2:+5435361 Msa0751870:CDS
ACTATCACTGTCTGGGAAAC+GGG 0.372098 5_2:+5435713 Msa0751870:three_prime_UTR
AGTTAAAAGGTCTTCATAGT+TGG 0.372121 5_2:-5435288 None:intergenic
AGTGATAGTATAAGCCTATA+TGG 0.384109 5_2:-5435699 None:intergenic
TGCCAGAGAAGAAGAATAGA+AGG 0.388717 5_2:+5435826 Msa0751870:three_prime_UTR
ACTCAAAGTTTACTGGAATT+GGG 0.389579 5_2:+5435435 Msa0751870:CDS
TACAAGGTTGATTCTTGAAC+TGG 0.391876 5_2:-5435217 None:intergenic
GAAGAAGATACTTGATTTGA+AGG 0.399726 5_2:-5434107 None:intergenic
AGGCTTATACTATCACTGTC+TGG 0.401288 5_2:+5435705 Msa0751870:three_prime_UTR
GTTAAAAGGTCTTCATAGTT+GGG 0.403819 5_2:-5435287 None:intergenic
CACGTGCACCGACAGTGTTT+TGG 0.406240 5_2:-5434005 None:intergenic
TACTATCACTGTCTGGGAAA+CGG 0.423338 5_2:+5435712 Msa0751870:three_prime_UTR
TGCTCATTGCTCTTGTTAGT+TGG 0.425769 5_2:+5435593 Msa0751870:CDS
AGCATCCTCACGCTCAAAAC+AGG 0.435655 5_2:-5435625 None:intergenic
CTTTATCACCAAAATTGGAT+TGG 0.444400 5_2:+5435565 Msa0751870:CDS
TCAAACTTTGAAGTAACAAA+TGG 0.445254 5_2:+5434131 Msa0751870:exon
AACAACAATCCAATTATCTT+CGG 0.461927 5_2:-5435465 None:intergenic
CTCGTCATCACTAGAACTCT+CGG 0.472274 5_2:-5435525 None:intergenic
AGTGATGACGAGACTTCTAA+TGG 0.482206 5_2:+5435536 Msa0751870:CDS
AAGTTTACTGGAATTGGGAT+TGG 0.486609 5_2:+5435440 Msa0751870:CDS
ACAATGTCCGGAACAAGGCC+AGG 0.505794 5_2:-5435368 None:intergenic
TCTTCATAGTTGGGTAATTG+AGG 0.505804 5_2:-5435278 None:intergenic
TACAGATCCATCCCTTGTGT+TGG 0.505945 5_2:-5435330 None:intergenic
TTGCAGCATAGTCGGTAAGC+AGG 0.507546 5_2:+5435668 Msa0751870:three_prime_UTR
GCACCTGTGGTATTTGTACA+AGG 0.509795 5_2:-5435233 None:intergenic
AATATTTCCAACACAAGGGA+TGG 0.513314 5_2:+5435323 Msa0751870:CDS
TTCTCGACACATCTTATGCA+TGG 0.516308 5_2:+5433913 Msa0751870:five_prime_UTR
ACAAGGCCAGGAACACAAGC+AGG 0.520245 5_2:-5435356 None:intergenic
AGTTTAGTTTAGTTTAGCAC+AGG 0.522901 5_2:-5434031 None:intergenic
GGCTTATACTATCACTGTCT+GGG 0.523682 5_2:+5435706 Msa0751870:three_prime_UTR
TATAAGCCTATATGGTAGCT+TGG 0.531909 5_2:-5435691 None:intergenic
GAAGATACTTGATTTGAAGG+TGG 0.540708 5_2:-5434104 None:intergenic
AAATTAGAGAGGACAATGTC+CGG 0.542734 5_2:-5435380 None:intergenic
GGTAATTGAGGCTCAGTACC+TGG 0.547704 5_2:-5435266 None:intergenic
AGCAGGCCAAGCTACCATAT+AGG 0.557003 5_2:+5435685 Msa0751870:three_prime_UTR
TGAGTGACTGAATAAGAGAA+TGG 0.560522 5_2:-5434190 None:intergenic
ATCATCAAATAATTAAGCAA+TGG 0.567763 5_2:-5436009 None:intergenic
ACTTAAATATTTCCAACACA+AGG 0.568858 5_2:+5435318 Msa0751870:CDS
TTCAGTGAAGCATAGAGGGA+TGG 0.576470 5_2:+5435764 Msa0751870:three_prime_UTR
TGGTATTGTGTTAGCACCTG+TGG 0.587893 5_2:-5435246 None:intergenic
AAACTTATTTGGTACTTCTG+CGG 0.593037 5_2:+5434054 Msa0751870:five_prime_UTR
TCGTCATCACTAGAACTCTC+GGG 0.593940 5_2:-5435524 None:intergenic
GAGGAAGATGATCTTTATCA+GGG 0.594123 5_2:+5435110 Msa0751870:CDS
GAGATTTCGTGAAATTAGAG+AGG 0.595369 5_2:-5435391 None:intergenic
CACTCAGTTCATTGTGACCA+TGG 0.595566 5_2:+5434207 Msa0751870:CDS
CGGGAAACTGCAAATAGTGA+TGG 0.598265 5_2:+5435732 Msa0751870:three_prime_UTR
CAACCTTGTACAAATACCAC+AGG 0.614385 5_2:+5435230 Msa0751870:CDS
AGAGGACAATGTCCGGAACA+AGG 0.617052 5_2:-5435373 None:intergenic
ACAGGTGCTAACACAATACC+AGG 0.623473 5_2:+5435248 Msa0751870:CDS
CTTAAATATTTCCAACACAA+GGG 0.640170 5_2:+5435319 Msa0751870:CDS
GCTTCCGTCAGAAGTGCTTG+TGG 0.642715 5_2:-5435495 None:intergenic
TTGACCACAAGCACTTCTGA+CGG 0.644420 5_2:+5435491 Msa0751870:CDS
GAACACAGCCAAAACACTGT+CGG 0.660789 5_2:+5433997 Msa0751870:five_prime_UTR
ATGTCAAGTTCAATGATGTG+CGG 0.702075 5_2:-5435145 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAACCAAAATATAATATAT+AGG + chr5_2:5434365-5434384 Msa0751870:intron 10.0%
!! ATAACCAAAATATAATATAT+AGG + chr5_2:5434365-5434384 Msa0751870:intron 10.0%
!! TAAACTAAACTAAACTTATT+TGG + chr5_2:5434043-5434062 Msa0751870:five_prime_UTR 15.0%
!!! AAAGCCTATATATTATATTT+TGG - chr5_2:5434372-5434391 None:intergenic 15.0%
!!! CTTGTATTTTAATTTCAAAA+TGG + chr5_2:5435981-5436000 Msa0751870:three_prime_UTR 15.0%
!! TAAACTAAACTAAACTTATT+TGG + chr5_2:5434043-5434062 Msa0751870:five_prime_UTR 15.0%
!!! AAAGCCTATATATTATATTT+TGG - chr5_2:5434372-5434391 None:intergenic 15.0%
!!! CTTGTATTTTAATTTCAAAA+TGG + chr5_2:5435981-5436000 Msa0751870:three_prime_UTR 15.0%
!!! TATTTTTGGTTTTGTTTTGA+TGG - chr5_2:5433970-5433989 None:intergenic 20.0%
!!! TAAAGACTTTTACTATAAGT+TGG + chr5_2:5434554-5434573 Msa0751870:intron 20.0%
!!! AAAGACTTTTACTATAAGTT+GGG + chr5_2:5434555-5434574 Msa0751870:intron 20.0%
!! TTAAGTGACATTTAGTTAAA+AGG - chr5_2:5435304-5435323 None:intergenic 20.0%
!!! TAATTTTGCTTCAATATTGT+CGG + chr5_2:5435792-5435811 Msa0751870:three_prime_UTR 20.0%
!!! AAAAAGCTGTTTTTTTATGT+AGG + chr5_2:5435851-5435870 Msa0751870:three_prime_UTR 20.0%
!!! AAAAGCTGTTTTTTTATGTA+GGG + chr5_2:5435852-5435871 Msa0751870:three_prime_UTR 20.0%
!! ACAATAAATTGTTCTATTGA+TGG + chr5_2:5435951-5435970 Msa0751870:three_prime_UTR 20.0%
!!! TATTTTTGGTTTTGTTTTGA+TGG - chr5_2:5433970-5433989 None:intergenic 20.0%
!!! TAAAGACTTTTACTATAAGT+TGG + chr5_2:5434554-5434573 Msa0751870:intron 20.0%
!!! AAAGACTTTTACTATAAGTT+GGG + chr5_2:5434555-5434574 Msa0751870:intron 20.0%
!! TTAAGTGACATTTAGTTAAA+AGG - chr5_2:5435304-5435323 None:intergenic 20.0%
!!! TAATTTTGCTTCAATATTGT+CGG + chr5_2:5435792-5435811 Msa0751870:three_prime_UTR 20.0%
!!! AAAAAGCTGTTTTTTTATGT+AGG + chr5_2:5435851-5435870 Msa0751870:three_prime_UTR 20.0%
!!! AAAAGCTGTTTTTTTATGTA+GGG + chr5_2:5435852-5435871 Msa0751870:three_prime_UTR 20.0%
!! ACAATAAATTGTTCTATTGA+TGG + chr5_2:5435951-5435970 Msa0751870:three_prime_UTR 20.0%
!!! TTTCTTCTTCGATTTTGTTT+TGG + chr5_2:5433868-5433887 Msa0751870:five_prime_UTR 25.0%
!! TGTGTTCAGGATATTATTTT+TGG - chr5_2:5433984-5434003 None:intergenic 25.0%
!!! TCAAACTTTGAAGTAACAAA+TGG + chr5_2:5434131-5434150 Msa0751870:exon 25.0%
! AATAGAAAGAATAGTTACCA+TGG - chr5_2:5434227-5434246 None:intergenic 25.0%
!!! TGCTTAACAACAAGTTTTTT+TGG + chr5_2:5434477-5434496 Msa0751870:intron 25.0%
! TTAAATGCTATTATCCACAA+TGG - chr5_2:5434537-5434556 None:intergenic 25.0%
! ACTAAAATTCAATCACACTA+TGG - chr5_2:5434925-5434944 None:intergenic 25.0%
! ACTTAAATATTTCCAACACA+AGG + chr5_2:5435318-5435337 Msa0751870:CDS 25.0%
! CTTAAATATTTCCAACACAA+GGG + chr5_2:5435319-5435338 Msa0751870:CDS 25.0%
! AACAACAATCCAATTATCTT+CGG - chr5_2:5435468-5435487 None:intergenic 25.0%
!!! TTTCTTCTTCGATTTTGTTT+TGG + chr5_2:5433868-5433887 Msa0751870:five_prime_UTR 25.0%
!! TGTGTTCAGGATATTATTTT+TGG - chr5_2:5433984-5434003 None:intergenic 25.0%
!!! TCAAACTTTGAAGTAACAAA+TGG + chr5_2:5434131-5434150 Msa0751870:exon 25.0%
! AATAGAAAGAATAGTTACCA+TGG - chr5_2:5434227-5434246 None:intergenic 25.0%
!!! TGCTTAACAACAAGTTTTTT+TGG + chr5_2:5434477-5434496 Msa0751870:intron 25.0%
! TTAAATGCTATTATCCACAA+TGG - chr5_2:5434537-5434556 None:intergenic 25.0%
! ACTAAAATTCAATCACACTA+TGG - chr5_2:5434925-5434944 None:intergenic 25.0%
! ACTTAAATATTTCCAACACA+AGG + chr5_2:5435318-5435337 Msa0751870:CDS 25.0%
! CTTAAATATTTCCAACACAA+GGG + chr5_2:5435319-5435338 Msa0751870:CDS 25.0%
! AACAACAATCCAATTATCTT+CGG - chr5_2:5435468-5435487 None:intergenic 25.0%
! AGTTTAGTTTAGTTTAGCAC+AGG - chr5_2:5434034-5434053 None:intergenic 30.0%
AAACTTATTTGGTACTTCTG+CGG + chr5_2:5434054-5434073 Msa0751870:five_prime_UTR 30.0%
! GAAGAAGATACTTGATTTGA+AGG - chr5_2:5434110-5434129 None:intergenic 30.0%
ATAGTTAGATAGATCCATTG+TGG + chr5_2:5434520-5434539 Msa0751870:intron 30.0%
CTGTATTGAATCAACAAAAC+AGG - chr5_2:5434585-5434604 None:intergenic 30.0%
! AGATACATACAAAGCTTGTT+TGG + chr5_2:5434649-5434668 Msa0751870:intron 30.0%
! TAAGTTTTCTTTAGTTACCC+AGG - chr5_2:5434792-5434811 None:intergenic 30.0%
GTAAGTGTTTATGCTATAAG+CGG + chr5_2:5434872-5434891 Msa0751870:intron 30.0%
GTTAAAAGGTCTTCATAGTT+GGG - chr5_2:5435290-5435309 None:intergenic 30.0%
AGTTAAAAGGTCTTCATAGT+TGG - chr5_2:5435291-5435310 None:intergenic 30.0%
CTTAATGACTCAAAGTTTAC+TGG + chr5_2:5435428-5435447 Msa0751870:CDS 30.0%
ACTCAAAGTTTACTGGAATT+GGG + chr5_2:5435435-5435454 Msa0751870:CDS 30.0%
! GAAAGCTTTATCACCAAAAT+TGG + chr5_2:5435560-5435579 Msa0751870:CDS 30.0%
CTTTATCACCAAAATTGGAT+TGG + chr5_2:5435565-5435584 Msa0751870:CDS 30.0%
! CATTTTTATTGCAGCATAGT+CGG + chr5_2:5435660-5435679 Msa0751870:three_prime_UTR 30.0%
AGTGATAGTATAAGCCTATA+TGG - chr5_2:5435702-5435721 None:intergenic 30.0%
! AGTTTAGTTTAGTTTAGCAC+AGG - chr5_2:5434034-5434053 None:intergenic 30.0%
AAACTTATTTGGTACTTCTG+CGG + chr5_2:5434054-5434073 Msa0751870:five_prime_UTR 30.0%
! GAAGAAGATACTTGATTTGA+AGG - chr5_2:5434110-5434129 None:intergenic 30.0%
ATAGTTAGATAGATCCATTG+TGG + chr5_2:5434520-5434539 Msa0751870:intron 30.0%
CTGTATTGAATCAACAAAAC+AGG - chr5_2:5434585-5434604 None:intergenic 30.0%
! AGATACATACAAAGCTTGTT+TGG + chr5_2:5434649-5434668 Msa0751870:intron 30.0%
! TAAGTTTTCTTTAGTTACCC+AGG - chr5_2:5434792-5434811 None:intergenic 30.0%
GTAAGTGTTTATGCTATAAG+CGG + chr5_2:5434872-5434891 Msa0751870:intron 30.0%
GTTAAAAGGTCTTCATAGTT+GGG - chr5_2:5435290-5435309 None:intergenic 30.0%
AGTTAAAAGGTCTTCATAGT+TGG - chr5_2:5435291-5435310 None:intergenic 30.0%
CTTAATGACTCAAAGTTTAC+TGG + chr5_2:5435428-5435447 Msa0751870:CDS 30.0%
ACTCAAAGTTTACTGGAATT+GGG + chr5_2:5435435-5435454 Msa0751870:CDS 30.0%
! GAAAGCTTTATCACCAAAAT+TGG + chr5_2:5435560-5435579 Msa0751870:CDS 30.0%
CTTTATCACCAAAATTGGAT+TGG + chr5_2:5435565-5435584 Msa0751870:CDS 30.0%
! CATTTTTATTGCAGCATAGT+CGG + chr5_2:5435660-5435679 Msa0751870:three_prime_UTR 30.0%
AGTGATAGTATAAGCCTATA+TGG - chr5_2:5435702-5435721 None:intergenic 30.0%
!!! GGTTTTGTTTTGATGGTAGT+AGG - chr5_2:5433963-5433982 None:intergenic 35.0%
! GAAGATACTTGATTTGAAGG+TGG - chr5_2:5434107-5434126 None:intergenic 35.0%
TGAGTGACTGAATAAGAGAA+TGG - chr5_2:5434193-5434212 None:intergenic 35.0%
!! AGATCTAGCTAGTTCTACAT+TGG - chr5_2:5434302-5434321 None:intergenic 35.0%
TAGAACTAGCTAGATCTGAT+AGG + chr5_2:5434305-5434324 Msa0751870:intron 35.0%
AGAACTAGCTAGATCTGATA+GGG + chr5_2:5434306-5434325 Msa0751870:intron 35.0%
ACACACTTACGAGATTGTTT+AGG + chr5_2:5434702-5434721 Msa0751870:intron 35.0%
TTATTCCCATAAGCTTTCCT+GGG + chr5_2:5434772-5434791 Msa0751870:intron 35.0%
TTGAGTGAATCTATCTCTCT+TGG + chr5_2:5434967-5434986 Msa0751870:intron 35.0%
TGAGGAAGATGATCTTTATC+AGG + chr5_2:5435109-5435128 Msa0751870:CDS 35.0%
GAGGAAGATGATCTTTATCA+GGG + chr5_2:5435110-5435129 Msa0751870:CDS 35.0%
ATGTCAAGTTCAATGATGTG+CGG - chr5_2:5435148-5435167 None:intergenic 35.0%
! TACAAGGTTGATTCTTGAAC+TGG - chr5_2:5435220-5435239 None:intergenic 35.0%
TCTTCATAGTTGGGTAATTG+AGG - chr5_2:5435281-5435300 None:intergenic 35.0%
AATATTTCCAACACAAGGGA+TGG + chr5_2:5435323-5435342 Msa0751870:CDS 35.0%
AAATTAGAGAGGACAATGTC+CGG - chr5_2:5435383-5435402 None:intergenic 35.0%
GAGATTTCGTGAAATTAGAG+AGG - chr5_2:5435394-5435413 None:intergenic 35.0%
GACTCAAAGTTTACTGGAAT+TGG + chr5_2:5435434-5435453 Msa0751870:CDS 35.0%
AAGTTTACTGGAATTGGGAT+TGG + chr5_2:5435440-5435459 Msa0751870:CDS 35.0%
! GCAGTAAACCAATCCAATTT+TGG - chr5_2:5435576-5435595 None:intergenic 35.0%
TATAAGCCTATATGGTAGCT+TGG - chr5_2:5435694-5435713 None:intergenic 35.0%
!! TGTTTTTCAGTGAAGCATAG+AGG + chr5_2:5435759-5435778 Msa0751870:three_prime_UTR 35.0%
!! GTTTTTCAGTGAAGCATAGA+GGG + chr5_2:5435760-5435779 Msa0751870:three_prime_UTR 35.0%
TTCCTTCTATTCTTCTTCTC+TGG - chr5_2:5435831-5435850 None:intergenic 35.0%
!!! GGTTTTGTTTTGATGGTAGT+AGG - chr5_2:5433963-5433982 None:intergenic 35.0%
! GAAGATACTTGATTTGAAGG+TGG - chr5_2:5434107-5434126 None:intergenic 35.0%
TGAGTGACTGAATAAGAGAA+TGG - chr5_2:5434193-5434212 None:intergenic 35.0%
!! AGATCTAGCTAGTTCTACAT+TGG - chr5_2:5434302-5434321 None:intergenic 35.0%
TAGAACTAGCTAGATCTGAT+AGG + chr5_2:5434305-5434324 Msa0751870:intron 35.0%
AGAACTAGCTAGATCTGATA+GGG + chr5_2:5434306-5434325 Msa0751870:intron 35.0%
ACACACTTACGAGATTGTTT+AGG + chr5_2:5434702-5434721 Msa0751870:intron 35.0%
TTATTCCCATAAGCTTTCCT+GGG + chr5_2:5434772-5434791 Msa0751870:intron 35.0%
TTGAGTGAATCTATCTCTCT+TGG + chr5_2:5434967-5434986 Msa0751870:intron 35.0%
TGAGGAAGATGATCTTTATC+AGG + chr5_2:5435109-5435128 Msa0751870:CDS 35.0%
GAGGAAGATGATCTTTATCA+GGG + chr5_2:5435110-5435129 Msa0751870:CDS 35.0%
ATGTCAAGTTCAATGATGTG+CGG - chr5_2:5435148-5435167 None:intergenic 35.0%
! TACAAGGTTGATTCTTGAAC+TGG - chr5_2:5435220-5435239 None:intergenic 35.0%
TCTTCATAGTTGGGTAATTG+AGG - chr5_2:5435281-5435300 None:intergenic 35.0%
AATATTTCCAACACAAGGGA+TGG + chr5_2:5435323-5435342 Msa0751870:CDS 35.0%
AAATTAGAGAGGACAATGTC+CGG - chr5_2:5435383-5435402 None:intergenic 35.0%
GAGATTTCGTGAAATTAGAG+AGG - chr5_2:5435394-5435413 None:intergenic 35.0%
GACTCAAAGTTTACTGGAAT+TGG + chr5_2:5435434-5435453 Msa0751870:CDS 35.0%
AAGTTTACTGGAATTGGGAT+TGG + chr5_2:5435440-5435459 Msa0751870:CDS 35.0%
! GCAGTAAACCAATCCAATTT+TGG - chr5_2:5435576-5435595 None:intergenic 35.0%
TATAAGCCTATATGGTAGCT+TGG - chr5_2:5435694-5435713 None:intergenic 35.0%
!! TGTTTTTCAGTGAAGCATAG+AGG + chr5_2:5435759-5435778 Msa0751870:three_prime_UTR 35.0%
!! GTTTTTCAGTGAAGCATAGA+GGG + chr5_2:5435760-5435779 Msa0751870:three_prime_UTR 35.0%
TTCCTTCTATTCTTCTTCTC+TGG - chr5_2:5435831-5435850 None:intergenic 35.0%
ATGTGTCGAGAAAAACATCG+AGG - chr5_2:5433905-5433924 None:intergenic 40.0%
TTCTCGACACATCTTATGCA+TGG + chr5_2:5433913-5433932 Msa0751870:five_prime_UTR 40.0%
GAACTAGCTAGATCTGATAG+GGG + chr5_2:5434307-5434326 Msa0751870:intron 40.0%
CTTATTCCCATAAGCTTTCC+TGG + chr5_2:5434771-5434790 Msa0751870:intron 40.0%
AGTTACCCAGGAAAGCTTAT+GGG - chr5_2:5434780-5434799 None:intergenic 40.0%
TAGTTACCCAGGAAAGCTTA+TGG - chr5_2:5434781-5434800 None:intergenic 40.0%
AGTGAATCTATCTCTCTTGG+TGG + chr5_2:5434970-5434989 Msa0751870:intron 40.0%
CAACCTTGTACAAATACCAC+AGG + chr5_2:5435230-5435249 Msa0751870:CDS 40.0%
!! GGATTGGTTCCGAAGATAAT+TGG + chr5_2:5435456-5435475 Msa0751870:CDS 40.0%
! AGTGATGACGAGACTTCTAA+TGG + chr5_2:5435536-5435555 Msa0751870:CDS 40.0%
TGCTCATTGCTCTTGTTAGT+TGG + chr5_2:5435593-5435612 Msa0751870:CDS 40.0%
!! ATTATCCTGTTTTGAGCGTG+AGG + chr5_2:5435620-5435639 Msa0751870:exon 40.0%
AGGCTTATACTATCACTGTC+TGG + chr5_2:5435705-5435724 Msa0751870:three_prime_UTR 40.0%
GGCTTATACTATCACTGTCT+GGG + chr5_2:5435706-5435725 Msa0751870:three_prime_UTR 40.0%
TACTATCACTGTCTGGGAAA+CGG + chr5_2:5435712-5435731 Msa0751870:three_prime_UTR 40.0%
TGCCAGAGAAGAAGAATAGA+AGG + chr5_2:5435826-5435845 Msa0751870:three_prime_UTR 40.0%
ATGTGTCGAGAAAAACATCG+AGG - chr5_2:5433905-5433924 None:intergenic 40.0%
TTCTCGACACATCTTATGCA+TGG + chr5_2:5433913-5433932 Msa0751870:five_prime_UTR 40.0%
GAACTAGCTAGATCTGATAG+GGG + chr5_2:5434307-5434326 Msa0751870:intron 40.0%
CTTATTCCCATAAGCTTTCC+TGG + chr5_2:5434771-5434790 Msa0751870:intron 40.0%
AGTTACCCAGGAAAGCTTAT+GGG - chr5_2:5434780-5434799 None:intergenic 40.0%
TAGTTACCCAGGAAAGCTTA+TGG - chr5_2:5434781-5434800 None:intergenic 40.0%
AGTGAATCTATCTCTCTTGG+TGG + chr5_2:5434970-5434989 Msa0751870:intron 40.0%
CAACCTTGTACAAATACCAC+AGG + chr5_2:5435230-5435249 Msa0751870:CDS 40.0%
!! GGATTGGTTCCGAAGATAAT+TGG + chr5_2:5435456-5435475 Msa0751870:CDS 40.0%
! AGTGATGACGAGACTTCTAA+TGG + chr5_2:5435536-5435555 Msa0751870:CDS 40.0%
TGCTCATTGCTCTTGTTAGT+TGG + chr5_2:5435593-5435612 Msa0751870:CDS 40.0%
!! ATTATCCTGTTTTGAGCGTG+AGG + chr5_2:5435620-5435639 Msa0751870:exon 40.0%
AGGCTTATACTATCACTGTC+TGG + chr5_2:5435705-5435724 Msa0751870:three_prime_UTR 40.0%
GGCTTATACTATCACTGTCT+GGG + chr5_2:5435706-5435725 Msa0751870:three_prime_UTR 40.0%
TACTATCACTGTCTGGGAAA+CGG + chr5_2:5435712-5435731 Msa0751870:three_prime_UTR 40.0%
TGCCAGAGAAGAAGAATAGA+AGG + chr5_2:5435826-5435845 Msa0751870:three_prime_UTR 40.0%
!!! ACAGTGTTTTGGCTGTGTTC+AGG - chr5_2:5433997-5434016 None:intergenic 45.0%
GAACACAGCCAAAACACTGT+CGG + chr5_2:5433997-5434016 Msa0751870:five_prime_UTR 45.0%
CACTCAGTTCATTGTGACCA+TGG + chr5_2:5434207-5434226 Msa0751870:CDS 45.0%
AGCGATAAGATCAGCGAATC+TGG + chr5_2:5434603-5434622 Msa0751870:intron 45.0%
! ATCTATCTCTCTTGGTGGAC+TGG + chr5_2:5434975-5434994 Msa0751870:intron 45.0%
ATCATCTTCCTCATCTGCAG+GGG - chr5_2:5435102-5435121 None:intergenic 45.0%
GATCATCTTCCTCATCTGCA+GGG - chr5_2:5435103-5435122 None:intergenic 45.0%
AGATCATCTTCCTCATCTGC+AGG - chr5_2:5435104-5435123 None:intergenic 45.0%
GCACCTGTGGTATTTGTACA+AGG - chr5_2:5435236-5435255 None:intergenic 45.0%
TGGTATTGTGTTAGCACCTG+TGG - chr5_2:5435249-5435268 None:intergenic 45.0%
ACAGGTGCTAACACAATACC+AGG + chr5_2:5435248-5435267 Msa0751870:CDS 45.0%
TACAGATCCATCCCTTGTGT+TGG - chr5_2:5435333-5435352 None:intergenic 45.0%
TTGACCACAAGCACTTCTGA+CGG + chr5_2:5435491-5435510 Msa0751870:CDS 45.0%
TCGTCATCACTAGAACTCTC+GGG - chr5_2:5435527-5435546 None:intergenic 45.0%
CTCGTCATCACTAGAACTCT+CGG - chr5_2:5435528-5435547 None:intergenic 45.0%
ACTATCACTGTCTGGGAAAC+GGG + chr5_2:5435713-5435732 Msa0751870:three_prime_UTR 45.0%
CGGGAAACTGCAAATAGTGA+TGG + chr5_2:5435732-5435751 Msa0751870:three_prime_UTR 45.0%
TTCAGTGAAGCATAGAGGGA+TGG + chr5_2:5435764-5435783 Msa0751870:three_prime_UTR 45.0%
!!! ACAGTGTTTTGGCTGTGTTC+AGG - chr5_2:5433997-5434016 None:intergenic 45.0%
GAACACAGCCAAAACACTGT+CGG + chr5_2:5433997-5434016 Msa0751870:five_prime_UTR 45.0%
CACTCAGTTCATTGTGACCA+TGG + chr5_2:5434207-5434226 Msa0751870:CDS 45.0%
AGCGATAAGATCAGCGAATC+TGG + chr5_2:5434603-5434622 Msa0751870:intron 45.0%
! ATCTATCTCTCTTGGTGGAC+TGG + chr5_2:5434975-5434994 Msa0751870:intron 45.0%
ATCATCTTCCTCATCTGCAG+GGG - chr5_2:5435102-5435121 None:intergenic 45.0%
GATCATCTTCCTCATCTGCA+GGG - chr5_2:5435103-5435122 None:intergenic 45.0%
AGATCATCTTCCTCATCTGC+AGG - chr5_2:5435104-5435123 None:intergenic 45.0%
GCACCTGTGGTATTTGTACA+AGG - chr5_2:5435236-5435255 None:intergenic 45.0%
TGGTATTGTGTTAGCACCTG+TGG - chr5_2:5435249-5435268 None:intergenic 45.0%
ACAGGTGCTAACACAATACC+AGG + chr5_2:5435248-5435267 Msa0751870:CDS 45.0%
TACAGATCCATCCCTTGTGT+TGG - chr5_2:5435333-5435352 None:intergenic 45.0%
TTGACCACAAGCACTTCTGA+CGG + chr5_2:5435491-5435510 Msa0751870:CDS 45.0%
TCGTCATCACTAGAACTCTC+GGG - chr5_2:5435527-5435546 None:intergenic 45.0%
CTCGTCATCACTAGAACTCT+CGG - chr5_2:5435528-5435547 None:intergenic 45.0%
ACTATCACTGTCTGGGAAAC+GGG + chr5_2:5435713-5435732 Msa0751870:three_prime_UTR 45.0%
CGGGAAACTGCAAATAGTGA+TGG + chr5_2:5435732-5435751 Msa0751870:three_prime_UTR 45.0%
TTCAGTGAAGCATAGAGGGA+TGG + chr5_2:5435764-5435783 Msa0751870:three_prime_UTR 45.0%
GGACTGGATCAAGCACAAGT+TGG + chr5_2:5434991-5435010 Msa0751870:intron 50.0%
TCATCTTCCTCATCTGCAGG+GGG - chr5_2:5435101-5435120 None:intergenic 50.0%
GGTAATTGAGGCTCAGTACC+TGG - chr5_2:5435269-5435288 None:intergenic 50.0%
GTAATGCCTGCTTGTGTTCC+TGG + chr5_2:5435350-5435369 Msa0751870:CDS 50.0%
!! TTGTGTTCCTGGCCTTGTTC+CGG + chr5_2:5435361-5435380 Msa0751870:CDS 50.0%
AGAGGACAATGTCCGGAACA+AGG - chr5_2:5435376-5435395 None:intergenic 50.0%
AGCATCCTCACGCTCAAAAC+AGG - chr5_2:5435628-5435647 None:intergenic 50.0%
TTGCAGCATAGTCGGTAAGC+AGG + chr5_2:5435668-5435687 Msa0751870:three_prime_UTR 50.0%
AGCAGGCCAAGCTACCATAT+AGG + chr5_2:5435685-5435704 Msa0751870:three_prime_UTR 50.0%
GGACTGGATCAAGCACAAGT+TGG + chr5_2:5434991-5435010 Msa0751870:intron 50.0%
TCATCTTCCTCATCTGCAGG+GGG - chr5_2:5435101-5435120 None:intergenic 50.0%
GGTAATTGAGGCTCAGTACC+TGG - chr5_2:5435269-5435288 None:intergenic 50.0%
GTAATGCCTGCTTGTGTTCC+TGG + chr5_2:5435350-5435369 Msa0751870:CDS 50.0%
!! TTGTGTTCCTGGCCTTGTTC+CGG + chr5_2:5435361-5435380 Msa0751870:CDS 50.0%
AGAGGACAATGTCCGGAACA+AGG - chr5_2:5435376-5435395 None:intergenic 50.0%
AGCATCCTCACGCTCAAAAC+AGG - chr5_2:5435628-5435647 None:intergenic 50.0%
TTGCAGCATAGTCGGTAAGC+AGG + chr5_2:5435668-5435687 Msa0751870:three_prime_UTR 50.0%
AGCAGGCCAAGCTACCATAT+AGG + chr5_2:5435685-5435704 Msa0751870:three_prime_UTR 50.0%
!! CACGTGCACCGACAGTGTTT+TGG - chr5_2:5434008-5434027 None:intergenic 55.0%
CATCTGCAGGGGGGATGTTA+AGG - chr5_2:5435091-5435110 None:intergenic 55.0%
TAACATCCCCCCTGCAGATG+AGG + chr5_2:5435091-5435110 Msa0751870:intron 55.0%
CATCTTCCTCATCTGCAGGG+GGG - chr5_2:5435100-5435119 None:intergenic 55.0%
ACAAGGCCAGGAACACAAGC+AGG - chr5_2:5435359-5435378 None:intergenic 55.0%
ACAATGTCCGGAACAAGGCC+AGG - chr5_2:5435371-5435390 None:intergenic 55.0%
!! GCTTCCGTCAGAAGTGCTTG+TGG - chr5_2:5435498-5435517 None:intergenic 55.0%
!! CACGTGCACCGACAGTGTTT+TGG - chr5_2:5434008-5434027 None:intergenic 55.0%
CATCTGCAGGGGGGATGTTA+AGG - chr5_2:5435091-5435110 None:intergenic 55.0%
TAACATCCCCCCTGCAGATG+AGG + chr5_2:5435091-5435110 Msa0751870:intron 55.0%
CATCTTCCTCATCTGCAGGG+GGG - chr5_2:5435100-5435119 None:intergenic 55.0%
ACAAGGCCAGGAACACAAGC+AGG - chr5_2:5435359-5435378 None:intergenic 55.0%
ACAATGTCCGGAACAAGGCC+AGG - chr5_2:5435371-5435390 None:intergenic 55.0%
!! GCTTCCGTCAGAAGTGCTTG+TGG - chr5_2:5435498-5435517 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 5433849 5436017 5433849 ID=Msa0751870;Name=Msa0751870
chr5_2 mRNA 5433849 5436017 5433849 ID=Msa0751870-mRNA-1;Parent=Msa0751870;Name=Msa0751870-mRNA-1;_AED=0.06;_eAED=0.06;_QI=301|1|1|1|0|0.5|2|383|201
chr5_2 exon 5433849 5434228 5433849 ID=Msa0751870-mRNA-1:exon:2153;Parent=Msa0751870-mRNA-1
chr5_2 exon 5435108 5436017 5435108 ID=Msa0751870-mRNA-1:exon:2154;Parent=Msa0751870-mRNA-1
chr5_2 five_prime_UTR 5433849 5434149 5433849 ID=Msa0751870-mRNA-1:five_prime_utr;Parent=Msa0751870-mRNA-1
chr5_2 CDS 5434150 5434228 5434150 ID=Msa0751870-mRNA-1:cds;Parent=Msa0751870-mRNA-1
chr5_2 CDS 5435108 5435634 5435108 ID=Msa0751870-mRNA-1:cds;Parent=Msa0751870-mRNA-1
chr5_2 three_prime_UTR 5435635 5436017 5435635 ID=Msa0751870-mRNA-1:three_prime_utr;Parent=Msa0751870-mRNA-1
Gene Sequence

>Msa0751870

ATGGCATTTTTTCGCTTCTCTCTTCTTTTCTTCTTTTCTTCCATTCTCTTATTCAGTCACTCAGTTCATTGTGACCATGATGAGGAAGATGATCTTTATCAGGGAGTCAACATGTACCGCACATCATTGAACTTGACATCGCTAACAAAGAACGAAAATGCTAATTGTTTTGCTGAAAAATTAGCTGACCAGTTCAAGAATCAACCTTGTACAAATACCACAGGTGCTAACACAATACCAGGTACTGAGCCTCAATTACCCAACTATGAAGACCTTTTAACTAAATGTCACTTAAATATTTCCAACACAAGGGATGGATCTGTAATGCCTGCTTGTGTTCCTGGCCTTGTTCCGGACATTGTCCTCTCTAATTTCACGAAATCTCTTTACTCGCAAAATCTTAATGACTCAAAGTTTACTGGAATTGGGATTGGTTCCGAAGATAATTGGATTGTTGTTGTATTGACCACAAGCACTTCTGACGGAAGCTTTGTTCCCGAGAGTTCTAGTGATGACGAGACTTCTAATGGTGAAAGCTTTATCACCAAAATTGGATTGGTTTACTGCTCATTGCTCTTGTTAGTTGGTAACATTATCCTGTTTTGA
Protein sequence

>Msa0751870

MAFFRFSLLFFFSSILLFSHSVHCDHDEEDDLYQGVNMYRTSLNLTSLTKNENANCFAEKLADQFKNQPCTNTTGANTIPGTEPQLPNYEDLLTKCHLNISNTRDGSVMPACVPGLVPDIVLSNFTKSLYSQNLNDSKFTGIGIGSEDNWIVVVLTTSTSDGSFVPESSSDDETSNGESFITKIGLVYCSLLLLVGNIILF*