AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09947


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09947.t1 MTR_5g012010 98.438 320 4 1 1 319 1 320 0.0 637
MS.gene09947.t1 MTR_2g012270 47.619 315 147 3 1 315 1 297 1.89e-105 310
MS.gene09947.t1 MTR_4g090900 50.000 296 129 4 1 295 1 278 3.30e-99 295
MS.gene09947.t1 MTR_1g056550 42.617 298 155 3 1 295 1 285 3.61e-85 259
MS.gene09947.t1 MTR_1g056560 48.583 247 126 1 49 295 44 289 1.12e-83 256
MS.gene09947.t1 MTR_2g088700 44.025 318 162 5 1 316 1 304 2.53e-80 246
MS.gene09947.t1 MTR_5g020670 46.823 299 131 7 1 294 1 276 2.68e-77 238
MS.gene09947.t1 MTR_7g106420 45.306 245 133 1 53 297 31 274 2.96e-76 236
MS.gene09947.t1 MTR_2g088700 44.781 297 148 5 1 295 1 283 5.91e-76 235
MS.gene09947.t1 MTR_6g028140 41.077 297 158 3 1 297 1 280 9.15e-73 227
MS.gene09947.t1 MTR_8g073900 36.813 182 75 5 1 178 1 146 2.05e-22 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09947.t1 AT1G08560 65.000 320 101 5 1 319 1 310 1.38e-132 379
MS.gene09947.t1 AT1G61290 47.923 313 141 4 1 311 1 293 2.31e-102 302
MS.gene09947.t1 AT1G61290 47.923 313 141 4 1 311 1 293 2.31e-102 302
MS.gene09947.t1 AT4G03330 47.619 315 150 3 1 315 1 300 1.41e-93 280
MS.gene09947.t1 AT1G11250 47.651 298 127 5 1 295 1 272 2.94e-90 271
MS.gene09947.t1 AT3G11820 50.192 261 129 1 35 295 29 288 4.06e-87 265
MS.gene09947.t1 AT3G11820 52.227 247 117 1 49 295 12 257 7.20e-87 263
MS.gene09947.t1 AT3G52400 43.289 298 159 3 1 297 1 289 2.10e-79 245
MS.gene09947.t1 AT2G18260 38.095 315 184 4 1 315 1 304 8.63e-70 219
MS.gene09947.t1 AT2G18260 38.095 315 184 4 1 315 1 304 8.63e-70 219
MS.gene09947.t1 AT3G03800 42.642 265 151 1 53 317 38 301 8.05e-68 214
MS.gene09947.t1 AT3G03800 42.642 265 151 1 53 317 38 301 8.05e-68 214
MS.gene09947.t1 AT5G08080 43.729 295 149 4 1 295 1 278 1.01e-66 211
MS.gene09947.t1 AT5G08080 43.729 295 149 4 1 295 1 278 1.01e-66 211
MS.gene09947.t1 AT5G08080 43.434 297 158 6 1 295 1 289 1.22e-65 209
MS.gene09947.t1 AT5G08080 39.171 217 115 4 1 217 1 200 5.38e-32 119
MS.gene09947.t1 AT5G08080 39.171 217 115 4 1 217 1 200 5.38e-32 119

Find 46 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGATTGAGAAGAGTTTAT+TGG 0.159342 5.2:-4471071 MS.gene09947:CDS
GGAGATGATGAAAGTTTCTT+AGG 0.183110 5.2:-4471172 MS.gene09947:CDS
GGAGCTTATGATGGAGTTTC+AGG 0.211805 5.2:-4471559 MS.gene09947:intron
TGAAGAAAGCAAAATGTATT+AGG 0.253459 5.2:-4471707 MS.gene09947:CDS
GCCATTAGTAACAGCAATTC+TGG 0.268624 5.2:+4471599 None:intergenic
AACTGATATGAATCTGTTCT+TGG 0.361438 5.2:-4471880 MS.gene09947:CDS
AAGATCTTCACTGACCCATT+TGG 0.382232 5.2:-4471904 MS.gene09947:CDS
AGAGAAAGTAAGAACAGAAA+TGG 0.386214 5.2:-4471850 MS.gene09947:CDS
ACATGGAAGAGGAAAGGTTC+TGG 0.392183 5.2:-4471134 MS.gene09947:CDS
TAGGACTCAACTTGAAGAAA+TGG 0.395349 5.2:-4471688 MS.gene09947:CDS
ACCAGAATTGCTGTTACTAA+TGG 0.398063 5.2:-4471600 MS.gene09947:CDS
GTATATTACAACAGGGGCTT+AGG 0.403888 5.2:-4471297 MS.gene09947:intron
ATTCTGGTTCTATAAATTGC+TGG 0.415838 5.2:+4471615 None:intergenic
GATTCATATCAGTTTCCAAA+TGG 0.439557 5.2:+4471889 None:intergenic
GATGAAGTTGATTTAGAAGC+TGG 0.444831 5.2:-4471957 MS.gene09947:CDS
GCAGGAAGTGGATGTGCATT+GGG 0.446829 5.2:-4470898 MS.gene09947:CDS
CTGCTGAAACTCACTACAAT+GGG 0.453019 5.2:+4470836 None:intergenic
GCTGCTAATAGAAGACTCTC+TGG 0.458709 5.2:-4471654 MS.gene09947:CDS
ATTGAGAAGATAATTTCAAA+TGG 0.459387 5.2:-4471193 MS.gene09947:CDS
GCTTCTCATTATGTTAAAGA+TGG 0.464968 5.2:-4470962 MS.gene09947:CDS
GAGATGATGAAAGTTTCTTA+GGG 0.469669 5.2:-4471171 MS.gene09947:CDS
ATTCATATCAGTTTCCAAAT+GGG 0.473520 5.2:+4471890 None:intergenic
AGCAGGAAGTGGATGTGCAT+TGG 0.476019 5.2:-4470899 MS.gene09947:CDS
AGAAGGTATTATACAGTGAC+AGG 0.477649 5.2:-4471238 MS.gene09947:CDS
ATCAGAAAAGTAGCAGGAAG+TGG 0.480407 5.2:-4470910 MS.gene09947:CDS
AACAGTATCAGAAAAGTAGC+AGG 0.504319 5.2:-4470916 MS.gene09947:CDS
TATGAATCTGTTCTTGGAAG+AGG 0.513466 5.2:-4471874 MS.gene09947:CDS
GAAACTCAAGGAGCTTATGA+TGG 0.521896 5.2:-4471568 MS.gene09947:CDS
GATGGTACAAAGAATCTTCA+CGG 0.525784 5.2:-4470944 MS.gene09947:CDS
GAGAAAGTAAGAACAGAAAT+GGG 0.532673 5.2:-4471849 MS.gene09947:CDS
AGTGAATACAAAGAAACAGT+AGG 0.536819 5.2:-4471262 MS.gene09947:CDS
TGATGAAAGTTTCTTAGGGA+AGG 0.540453 5.2:-4471167 MS.gene09947:CDS
AGACTCTCTGGACTTAAAGA+TGG 0.542560 5.2:-4471642 MS.gene09947:CDS
GCAATACAAGAACATGGAAG+AGG 0.566682 5.2:-4471145 MS.gene09947:CDS
ACTGCTGAAACTCACTACAA+TGG 0.567938 5.2:+4470835 None:intergenic
TGAAGCTTTGAAAGCACTGA+GGG 0.568866 5.2:-4471760 MS.gene09947:CDS
GTGAATACAAAGAAACAGTA+GGG 0.579417 5.2:-4471261 MS.gene09947:CDS
ACAAAGAAACAGTAGGGAGA+AGG 0.588838 5.2:-4471255 MS.gene09947:CDS
TGGCTTGCGTAAGAAACTCA+AGG 0.596215 5.2:-4471580 MS.gene09947:CDS
ACAAGAACATGGAAGAGGAA+AGG 0.606969 5.2:-4471140 MS.gene09947:CDS
GGGAAGGCAATACAAGAACA+TGG 0.609551 5.2:-4471151 MS.gene09947:CDS
AGGAAAGGTTCTGGAAACTG+TGG 0.665351 5.2:-4471125 MS.gene09947:CDS
GAAGGTATTATACAGTGACA+GGG 0.682716 5.2:-4471237 MS.gene09947:CDS
CTGAAGCTTTGAAAGCACTG+AGG 0.693842 5.2:-4471761 MS.gene09947:CDS
GCTGTTATGGTTGAAGCACA+AGG 0.705464 5.2:-4471022 MS.gene09947:CDS
AAGGTATTATACAGTGACAG+GGG 0.727159 5.2:-4471236 MS.gene09947:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATTTATGTATCAATTTTA+AGG - chr5.2:4471379-4471398 MS.gene09947:intron 15.0%
!! ATTGAGAAGATAATTTCAAA+TGG - chr5.2:4471656-4471675 MS.gene09947:CDS 20.0%
!!! TCATCAAATATTTTTGGATA+TGG - chr5.2:4471805-4471824 MS.gene09947:CDS 20.0%
!!! TTTTTTTAGATCAACATAGT+TGG + chr5.2:4470860-4470879 None:intergenic 20.0%
! AAACCTTGTATATTACAACA+GGG - chr5.2:4471545-4471564 MS.gene09947:intron 25.0%
! ATATTTGTGTAAGAAACTCA+TGG - chr5.2:4471414-4471433 MS.gene09947:intron 25.0%
! ATTCATATCAGTTTCCAAAT+GGG + chr5.2:4470962-4470981 None:intergenic 25.0%
! TGAAGAAAGCAAAATGTATT+AGG - chr5.2:4471142-4471161 MS.gene09947:CDS 25.0%
!! GATTATACTTTTGCTTATTC+TGG - chr5.2:4471973-4471992 MS.gene09947:CDS 25.0%
!! GGAACTTCATCAAATATTTT+TGG - chr5.2:4471799-4471818 MS.gene09947:CDS 25.0%
!!! ACAATGATGCTTTTTAGAAA+AGG + chr5.2:4471458-4471477 None:intergenic 25.0%
AACCTTGTATATTACAACAG+GGG - chr5.2:4471546-4471565 MS.gene09947:intron 30.0%
AACTGATATGAATCTGTTCT+TGG - chr5.2:4470969-4470988 MS.gene09947:CDS 30.0%
AGAGAAAGTAAGAACAGAAA+TGG - chr5.2:4470999-4471018 MS.gene09947:CDS 30.0%
AGAGATTGAGAAGAGTTTAT+TGG - chr5.2:4471778-4471797 MS.gene09947:CDS 30.0%
AGTGAATACAAAGAAACAGT+AGG - chr5.2:4471587-4471606 MS.gene09947:CDS 30.0%
GAAACCTTGTATATTACAAC+AGG - chr5.2:4471544-4471563 MS.gene09947:intron 30.0%
GAGAAAGTAAGAACAGAAAT+GGG - chr5.2:4471000-4471019 MS.gene09947:CDS 30.0%
GAGATGATGAAAGTTTCTTA+GGG - chr5.2:4471678-4471697 MS.gene09947:CDS 30.0%
GATTCATATCAGTTTCCAAA+TGG + chr5.2:4470963-4470982 None:intergenic 30.0%
GCTTCTCATTATGTTAAAGA+TGG - chr5.2:4471887-4471906 MS.gene09947:CDS 30.0%
GTGAATACAAAGAAACAGTA+GGG - chr5.2:4471588-4471607 MS.gene09947:CDS 30.0%
!! ATTCTGGTTCTATAAATTGC+TGG + chr5.2:4471237-4471256 None:intergenic 30.0%
!! ATTTTTGGATATGGCTGTTA+TGG - chr5.2:4471814-4471833 MS.gene09947:CDS 30.0%
!!! TCTTCTGGTTTTTTTCTTTC+TGG - chr5.2:4471333-4471352 MS.gene09947:intron 30.0%
AACAGTATCAGAAAAGTAGC+AGG - chr5.2:4471933-4471952 MS.gene09947:CDS 35.0%
AACCAGAAGAGAAATTGACA+AGG + chr5.2:4471323-4471342 None:intergenic 35.0%
ACCAGAAGAGAAATTGACAA+GGG + chr5.2:4471322-4471341 None:intergenic 35.0%
GATGGTACAAAGAATCTTCA+CGG - chr5.2:4471905-4471924 MS.gene09947:CDS 35.0%
GGAGATGATGAAAGTTTCTT+AGG - chr5.2:4471677-4471696 MS.gene09947:CDS 35.0%
TAGGACTCAACTTGAAGAAA+TGG - chr5.2:4471161-4471180 MS.gene09947:CDS 35.0%
TATGAATCTGTTCTTGGAAG+AGG - chr5.2:4470975-4470994 MS.gene09947:CDS 35.0%
TGAAGCACAAGGTGAAAAAA+TGG - chr5.2:4471838-4471857 MS.gene09947:CDS 35.0%
TGATGAAAGTTTCTTAGGGA+AGG - chr5.2:4471682-4471701 MS.gene09947:CDS 35.0%
! AAGGTATTATACAGTGACAG+GGG - chr5.2:4471613-4471632 MS.gene09947:CDS 35.0%
! ACCAGAATTGCTGTTACTAA+TGG - chr5.2:4471249-4471268 MS.gene09947:CDS 35.0%
! GATGAAGTTGATTTAGAAGC+TGG - chr5.2:4470892-4470911 MS.gene09947:CDS 35.0%
!! AGAAGGTATTATACAGTGAC+AGG - chr5.2:4471611-4471630 MS.gene09947:CDS 35.0%
!! GAAGGTATTATACAGTGACA+GGG - chr5.2:4471612-4471631 MS.gene09947:CDS 35.0%
AAGATCTTCACTGACCCATT+TGG - chr5.2:4470945-4470964 MS.gene09947:CDS 40.0%
ACAAAGAAACAGTAGGGAGA+AGG - chr5.2:4471594-4471613 MS.gene09947:CDS 40.0%
ACAAGAACATGGAAGAGGAA+AGG - chr5.2:4471709-4471728 MS.gene09947:CDS 40.0%
ACCCTTGTCAATTTCTCTTC+TGG - chr5.2:4471318-4471337 MS.gene09947:intron 40.0%
ACTGCTGAAACTCACTACAA+TGG + chr5.2:4472017-4472036 None:intergenic 40.0%
AGACTCTCTGGACTTAAAGA+TGG - chr5.2:4471207-4471226 MS.gene09947:CDS 40.0%
AGCCCCTGTTGTAATATACA+AGG + chr5.2:4471551-4471570 None:intergenic 40.0%
ATCAGAAAAGTAGCAGGAAG+TGG - chr5.2:4471939-4471958 MS.gene09947:CDS 40.0%
CTGCTGAAACTCACTACAAT+GGG + chr5.2:4472016-4472035 None:intergenic 40.0%
GAAACTCAAGGAGCTTATGA+TGG - chr5.2:4471281-4471300 MS.gene09947:CDS 40.0%
GCAATACAAGAACATGGAAG+AGG - chr5.2:4471704-4471723 MS.gene09947:CDS 40.0%
GCCATTAGTAACAGCAATTC+TGG + chr5.2:4471253-4471272 None:intergenic 40.0%
GTATATTACAACAGGGGCTT+AGG - chr5.2:4471552-4471571 MS.gene09947:intron 40.0%
!! GAAGCTGGTCTTCAAAAACA+AGG - chr5.2:4470907-4470926 MS.gene09947:CDS 40.0%
!! TGAAGCTTTGAAAGCACTGA+GGG - chr5.2:4471089-4471108 MS.gene09947:CDS 40.0%
ACATGGAAGAGGAAAGGTTC+TGG - chr5.2:4471715-4471734 MS.gene09947:CDS 45.0%
GCTGCTAATAGAAGACTCTC+TGG - chr5.2:4471195-4471214 MS.gene09947:CDS 45.0%
GCTGTTATGGTTGAAGCACA+AGG - chr5.2:4471827-4471846 MS.gene09947:CDS 45.0%
GGGAAGGCAATACAAGAACA+TGG - chr5.2:4471698-4471717 MS.gene09947:CDS 45.0%
! GGAGCTTATGATGGAGTTTC+AGG - chr5.2:4471290-4471309 MS.gene09947:intron 45.0%
! TGGCTTGCGTAAGAAACTCA+AGG - chr5.2:4471269-4471288 MS.gene09947:CDS 45.0%
!! AGGAAAGGTTCTGGAAACTG+TGG - chr5.2:4471724-4471743 MS.gene09947:CDS 45.0%
!! CTGAAGCTTTGAAAGCACTG+AGG - chr5.2:4471088-4471107 MS.gene09947:CDS 45.0%
!! GCTGGTCTTCAAAAACAAGG+TGG - chr5.2:4470910-4470929 MS.gene09947:CDS 45.0%
AGCAGGAAGTGGATGTGCAT+TGG - chr5.2:4471950-4471969 MS.gene09947:CDS 50.0%
GCAGGAAGTGGATGTGCATT+GGG - chr5.2:4471951-4471970 MS.gene09947:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 4470829 4472042 4470829 ID=MS.gene09947
chr5.2 mRNA 4470829 4472042 4470829 ID=MS.gene09947.t1;Parent=MS.gene09947
chr5.2 exon 4471560 4472042 4471560 ID=MS.gene09947.t1.exon1;Parent=MS.gene09947.t1
chr5.2 CDS 4471560 4472042 4471560 ID=cds.MS.gene09947.t1;Parent=MS.gene09947.t1
chr5.2 exon 4470829 4471305 4470829 ID=MS.gene09947.t1.exon2;Parent=MS.gene09947.t1
chr5.2 CDS 4470829 4471305 4470829 ID=cds.MS.gene09947.t1;Parent=MS.gene09947.t1
Gene Sequence

>MS.gene09947

ATGAATGATCTAATGACAAAATCATTCACCAACTATGTTGATCTAAAAAAAGCAGCTATGAAAGATGAAGTTGATTTAGAAGCTGGTCTTCAAAAACAAGGTGGTGTTGAATTAATAAGATCTTCACTGACCCATTTGGAAACTGATATGAATCTGTTCTTGGAAGAGGCAGAGAAAGTAAGAACAGAAATGGGTGAAATTCGCGATATACTAACAAAGCTTGAACAAGCAAATCAAGAGAGCAAGAGTCTTCACAAAACTGAAGCTTTGAAAGCACTGAGGGAAAGAATTAATACTGATATTGTAGCTGTTTTGAAGAAAGCAAAATGTATTAGGACTCAACTTGAAGAAATGGATCGTGCTAATGCTGCTAATAGAAGACTCTCTGGACTTAAAGATGGTTCACCAGCAATTTATAGAACCAGAATTGCTGTTACTAATGGCTTGCGTAAGAAACTCAAGGAGCTTATGATGGAGTTTCAGGTAGTTACCCTTGTCAATTTCTCTTCTGGTTTTTTTCTTTCTGGATTCAAATTAATAATTTTTTTTTGAATTTATGTATCAATTTTAAGGTTTAAAGTATGCATATTTGTGTAAGAAACTCATGGAATATGCATATCTGTGTGCCTTTTCTAAAAAGCATCATTGTTTTTTTTTTCTTCTTCTAGTGTTAGTATGTTGTGTATCTTGTAACATTACAATCATCACAATTTATGAAACCTTGTATATTACAACAGGGGCTTAGGCAGAAAATGATGAGTGAATACAAAGAAACAGTAGGGAGAAGGTATTATACAGTGACAGGGGAACATGCTGATGAAGAAGTAATTGAGAAGATAATTTCAAATGGAGATGATGAAAGTTTCTTAGGGAAGGCAATACAAGAACATGGAAGAGGAAAGGTTCTGGAAACTGTGGTAGAGATACAAGACAGACATGATGCTGCTAAAGAGATTGAGAAGAGTTTATTGGAACTTCATCAAATATTTTTGGATATGGCTGTTATGGTTGAAGCACAAGGTGAAAAAATGGATGATATTGAACATCATGTTCTTCATGCTTCTCATTATGTTAAAGATGGTACAAAGAATCTTCACGGTGCTAAACAGTATCAGAAAAGTAGCAGGAAGTGGATGTGCATTGGGATTATACTTTTGCTTATTCTGGTTCTCATCATTGTTATTCCCATTGTAGTGAGTTTCAGCAGTTCTTGA

Protein sequence

>MS.gene09947.t1

MNDLMTKSFTNYVDLKKAAMKDEVDLEAGLQKQGGVELIRSSLTHLETDMNLFLEEAEKVRTEMGEIRDILTKLEQANQESKSLHKTEALKALRERINTDIVAVLKKAKCIRTQLEEMDRANAANRRLSGLKDGSPAIYRTRIAVTNGLRKKLKELMMEFQGLRQKMMSEYKETVGRRYYTVTGEHADEEVIEKIISNGDDESFLGKAIQEHGRGKVLETVVEIQDRHDAAKEIEKSLLELHQIFLDMAVMVEAQGEKMDDIEHHVLHASHYVKDGTKNLHGAKQYQKSSRKWMCIGIILLLILVLIIVIPIVVSFSSS