AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21144


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21144.t1 MTR_5g012010 98.438 320 4 1 1 319 1 320 0.0 637
MS.gene21144.t1 MTR_2g012270 47.619 315 147 3 1 315 1 297 1.89e-105 310
MS.gene21144.t1 MTR_4g090900 50.000 296 129 4 1 295 1 278 3.30e-99 295
MS.gene21144.t1 MTR_1g056550 42.617 298 155 3 1 295 1 285 3.61e-85 259
MS.gene21144.t1 MTR_1g056560 48.583 247 126 1 49 295 44 289 1.12e-83 256
MS.gene21144.t1 MTR_2g088700 44.025 318 162 5 1 316 1 304 2.53e-80 246
MS.gene21144.t1 MTR_5g020670 46.823 299 131 7 1 294 1 276 2.68e-77 238
MS.gene21144.t1 MTR_7g106420 45.306 245 133 1 53 297 31 274 2.96e-76 236
MS.gene21144.t1 MTR_2g088700 44.781 297 148 5 1 295 1 283 5.91e-76 235
MS.gene21144.t1 MTR_6g028140 41.077 297 158 3 1 297 1 280 9.15e-73 227
MS.gene21144.t1 MTR_8g073900 36.813 182 75 5 1 178 1 146 2.05e-22 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21144.t1 AT1G08560 65.000 320 101 5 1 319 1 310 1.38e-132 379
MS.gene21144.t1 AT1G61290 47.923 313 141 4 1 311 1 293 2.31e-102 302
MS.gene21144.t1 AT1G61290 47.923 313 141 4 1 311 1 293 2.31e-102 302
MS.gene21144.t1 AT4G03330 47.619 315 150 3 1 315 1 300 1.41e-93 280
MS.gene21144.t1 AT1G11250 47.651 298 127 5 1 295 1 272 2.94e-90 271
MS.gene21144.t1 AT3G11820 50.192 261 129 1 35 295 29 288 4.06e-87 265
MS.gene21144.t1 AT3G11820 52.227 247 117 1 49 295 12 257 7.20e-87 263
MS.gene21144.t1 AT3G52400 43.289 298 159 3 1 297 1 289 2.10e-79 245
MS.gene21144.t1 AT2G18260 38.095 315 184 4 1 315 1 304 8.63e-70 219
MS.gene21144.t1 AT2G18260 38.095 315 184 4 1 315 1 304 8.63e-70 219
MS.gene21144.t1 AT3G03800 42.642 265 151 1 53 317 38 301 8.05e-68 214
MS.gene21144.t1 AT3G03800 42.642 265 151 1 53 317 38 301 8.05e-68 214
MS.gene21144.t1 AT5G08080 43.729 295 149 4 1 295 1 278 1.01e-66 211
MS.gene21144.t1 AT5G08080 43.729 295 149 4 1 295 1 278 1.01e-66 211
MS.gene21144.t1 AT5G08080 43.434 297 158 6 1 295 1 289 1.22e-65 209
MS.gene21144.t1 AT5G08080 39.171 217 115 4 1 217 1 200 5.38e-32 119
MS.gene21144.t1 AT5G08080 39.171 217 115 4 1 217 1 200 5.38e-32 119

Find 46 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGATTGAGAAGAGTTTAT+TGG 0.159342 5.1:-5419850 MS.gene21144:CDS
GGAGATGATGAAAGTTTCTT+AGG 0.183110 5.1:-5419951 MS.gene21144:CDS
GGAGCTTATGATGGAGTTTC+AGG 0.211805 5.1:-5420337 MS.gene21144:intron
TGAAGAAAGCAAAATGTATT+AGG 0.253459 5.1:-5420485 MS.gene21144:CDS
GCCATTAGTAACAGCAATTC+TGG 0.268624 5.1:+5420377 None:intergenic
AACTGATATGAATCTGTTCT+TGG 0.361438 5.1:-5420658 MS.gene21144:CDS
AAGATCTTCACTGACCCATT+TGG 0.382232 5.1:-5420682 MS.gene21144:CDS
AGAGAAAGTAAGAACAGAAA+TGG 0.386214 5.1:-5420628 MS.gene21144:CDS
ACATGGAAGAGGAAAGGTTC+TGG 0.392183 5.1:-5419913 MS.gene21144:CDS
TAGGACTCAACTTGAAGAAA+TGG 0.395349 5.1:-5420466 MS.gene21144:CDS
ACCAGAATTGCTGTTACTAA+TGG 0.398063 5.1:-5420378 MS.gene21144:CDS
ATTCTGGTTCTATAAATTGC+TGG 0.415838 5.1:+5420393 None:intergenic
GATTCATATCAGTTTCCAAA+TGG 0.439557 5.1:+5420667 None:intergenic
GATGAAGTTGATTTAGAAGC+TGG 0.444831 5.1:-5420735 MS.gene21144:CDS
GCAGGAAGTGGATGTGCATT+GGG 0.446829 5.1:-5419677 MS.gene21144:CDS
CTGCTGAAACTCACTACAAT+GGG 0.453019 5.1:+5419615 None:intergenic
GCTGCTAATAGAAGACTCTC+TGG 0.458709 5.1:-5420432 MS.gene21144:CDS
ATTGAGAAGATAATTTCAAA+TGG 0.459387 5.1:-5419972 MS.gene21144:CDS
GCTTCTCATTATGTTAAAGA+TGG 0.464968 5.1:-5419741 MS.gene21144:CDS
GTATATTACAACAGGGACTT+AGG 0.467475 5.1:-5420076 MS.gene21144:intron
GAGATGATGAAAGTTTCTTA+GGG 0.469669 5.1:-5419950 MS.gene21144:CDS
ATTCATATCAGTTTCCAAAT+GGG 0.473520 5.1:+5420668 None:intergenic
AGCAGGAAGTGGATGTGCAT+TGG 0.476019 5.1:-5419678 MS.gene21144:CDS
AGAAGGTATTATACAGTGAC+AGG 0.477649 5.1:-5420017 MS.gene21144:CDS
ATCAGAAAAGTAGCAGGAAG+TGG 0.480407 5.1:-5419689 MS.gene21144:CDS
AACAGTATCAGAAAAGTAGC+AGG 0.504319 5.1:-5419695 MS.gene21144:CDS
TATGAATCTGTTCTTGGAAG+AGG 0.513466 5.1:-5420652 MS.gene21144:CDS
GAAACTCAAGGAGCTTATGA+TGG 0.521896 5.1:-5420346 MS.gene21144:CDS
GATGGTACAAAGAATCTTCA+CGG 0.525784 5.1:-5419723 MS.gene21144:CDS
GAGAAAGTAAGAACAGAAAT+GGG 0.532673 5.1:-5420627 MS.gene21144:CDS
AGTGAATACAAAGAAACAGT+AGG 0.536819 5.1:-5420041 MS.gene21144:CDS
TGATGAAAGTTTCTTAGGGA+AGG 0.540453 5.1:-5419946 MS.gene21144:CDS
AGACTCTCTGGACTTAAAGA+TGG 0.542560 5.1:-5420420 MS.gene21144:CDS
GCAATACAAGAACATGGAAG+AGG 0.566682 5.1:-5419924 MS.gene21144:CDS
ACTGCTGAAACTCACTACAA+TGG 0.567938 5.1:+5419614 None:intergenic
TGAAGCTTTGAAAGCACTGA+GGG 0.568866 5.1:-5420538 MS.gene21144:CDS
GTGAATACAAAGAAACAGTA+GGG 0.579417 5.1:-5420040 MS.gene21144:CDS
ACAAAGAAACAGTAGGGAGA+AGG 0.588838 5.1:-5420034 MS.gene21144:CDS
TGGCTTGCGTAAGAAACTCA+AGG 0.596215 5.1:-5420358 MS.gene21144:CDS
ACAAGAACATGGAAGAGGAA+AGG 0.606969 5.1:-5419919 MS.gene21144:CDS
GGGAAGGCAATACAAGAACA+TGG 0.609551 5.1:-5419930 MS.gene21144:CDS
AGGAAAGGTTCTGGAAACTG+TGG 0.665351 5.1:-5419904 MS.gene21144:CDS
GAAGGTATTATACAGTGACA+GGG 0.682716 5.1:-5420016 MS.gene21144:CDS
CTGAAGCTTTGAAAGCACTG+AGG 0.693842 5.1:-5420539 MS.gene21144:CDS
GCTGTTATGGTTGAAGCACA+AGG 0.705464 5.1:-5419801 MS.gene21144:CDS
AAGGTATTATACAGTGACAG+GGG 0.727159 5.1:-5420015 MS.gene21144:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATTTATGTATCAATTTTA+AGG - chr5.1:5420158-5420177 MS.gene21144:intron 15.0%
!! ATTGAGAAGATAATTTCAAA+TGG - chr5.1:5420434-5420453 MS.gene21144:CDS 20.0%
!!! TCATCAAATATTTTTGGATA+TGG - chr5.1:5420583-5420602 MS.gene21144:CDS 20.0%
!!! TTTTTTTAGATCAACATAGT+TGG + chr5.1:5419639-5419658 None:intergenic 20.0%
! AAACCTTGTATATTACAACA+GGG - chr5.1:5420323-5420342 MS.gene21144:intron 25.0%
! ATATTTGTGTAAGAAACTCA+TGG - chr5.1:5420193-5420212 MS.gene21144:intron 25.0%
! ATTCATATCAGTTTCCAAAT+GGG + chr5.1:5419741-5419760 None:intergenic 25.0%
! TGAAGAAAGCAAAATGTATT+AGG - chr5.1:5419921-5419940 MS.gene21144:CDS 25.0%
!! GATTATACTTTTGCTTATTC+TGG - chr5.1:5420751-5420770 MS.gene21144:CDS 25.0%
!! GGAACTTCATCAAATATTTT+TGG - chr5.1:5420577-5420596 MS.gene21144:CDS 25.0%
!!! ACAATGATGCTTTTTAGAAA+AGG + chr5.1:5420237-5420256 None:intergenic 25.0%
AACTGATATGAATCTGTTCT+TGG - chr5.1:5419748-5419767 MS.gene21144:CDS 30.0%
AGAGAAAGTAAGAACAGAAA+TGG - chr5.1:5419778-5419797 MS.gene21144:CDS 30.0%
AGAGATTGAGAAGAGTTTAT+TGG - chr5.1:5420556-5420575 MS.gene21144:CDS 30.0%
AGTGAATACAAAGAAACAGT+AGG - chr5.1:5420365-5420384 MS.gene21144:CDS 30.0%
GAAACCTTGTATATTACAAC+AGG - chr5.1:5420322-5420341 MS.gene21144:intron 30.0%
GAGAAAGTAAGAACAGAAAT+GGG - chr5.1:5419779-5419798 MS.gene21144:CDS 30.0%
GAGATGATGAAAGTTTCTTA+GGG - chr5.1:5420456-5420475 MS.gene21144:CDS 30.0%
GATTCATATCAGTTTCCAAA+TGG + chr5.1:5419742-5419761 None:intergenic 30.0%
GCTTCTCATTATGTTAAAGA+TGG - chr5.1:5420665-5420684 MS.gene21144:CDS 30.0%
GTGAATACAAAGAAACAGTA+GGG - chr5.1:5420366-5420385 MS.gene21144:CDS 30.0%
!! ATTCTGGTTCTATAAATTGC+TGG + chr5.1:5420016-5420035 None:intergenic 30.0%
!! ATTTTTGGATATGGCTGTTA+TGG - chr5.1:5420592-5420611 MS.gene21144:CDS 30.0%
!!! TCTTCTGGTTTTTTTCTTTC+TGG - chr5.1:5420112-5420131 MS.gene21144:intron 30.0%
AACAGTATCAGAAAAGTAGC+AGG - chr5.1:5420711-5420730 MS.gene21144:CDS 35.0%
AACCAGAAGAGAAATTGACA+AGG + chr5.1:5420102-5420121 None:intergenic 35.0%
ACCAGAAGAGAAATTGACAA+GGG + chr5.1:5420101-5420120 None:intergenic 35.0%
AGTCCCTGTTGTAATATACA+AGG + chr5.1:5420329-5420348 None:intergenic 35.0%
GATGGTACAAAGAATCTTCA+CGG - chr5.1:5420683-5420702 MS.gene21144:CDS 35.0%
GGAGATGATGAAAGTTTCTT+AGG - chr5.1:5420455-5420474 MS.gene21144:CDS 35.0%
GTATATTACAACAGGGACTT+AGG - chr5.1:5420330-5420349 MS.gene21144:intron 35.0%
TAGGACTCAACTTGAAGAAA+TGG - chr5.1:5419940-5419959 MS.gene21144:CDS 35.0%
TATGAATCTGTTCTTGGAAG+AGG - chr5.1:5419754-5419773 MS.gene21144:CDS 35.0%
TGAAGCACAAGGTGAAAAAA+TGG - chr5.1:5420616-5420635 MS.gene21144:CDS 35.0%
TGATGAAAGTTTCTTAGGGA+AGG - chr5.1:5420460-5420479 MS.gene21144:CDS 35.0%
! AAGGTATTATACAGTGACAG+GGG - chr5.1:5420391-5420410 MS.gene21144:CDS 35.0%
! ACCAGAATTGCTGTTACTAA+TGG - chr5.1:5420028-5420047 MS.gene21144:CDS 35.0%
! GATGAAGTTGATTTAGAAGC+TGG - chr5.1:5419671-5419690 MS.gene21144:CDS 35.0%
!! AGAAGGTATTATACAGTGAC+AGG - chr5.1:5420389-5420408 MS.gene21144:CDS 35.0%
!! GAAGGTATTATACAGTGACA+GGG - chr5.1:5420390-5420409 MS.gene21144:CDS 35.0%
AAGATCTTCACTGACCCATT+TGG - chr5.1:5419724-5419743 MS.gene21144:CDS 40.0%
ACAAAGAAACAGTAGGGAGA+AGG - chr5.1:5420372-5420391 MS.gene21144:CDS 40.0%
ACAAGAACATGGAAGAGGAA+AGG - chr5.1:5420487-5420506 MS.gene21144:CDS 40.0%
ACCCTTGTCAATTTCTCTTC+TGG - chr5.1:5420097-5420116 MS.gene21144:intron 40.0%
ACTGCTGAAACTCACTACAA+TGG + chr5.1:5420795-5420814 None:intergenic 40.0%
AGACTCTCTGGACTTAAAGA+TGG - chr5.1:5419986-5420005 MS.gene21144:CDS 40.0%
ATCAGAAAAGTAGCAGGAAG+TGG - chr5.1:5420717-5420736 MS.gene21144:CDS 40.0%
CTGCTGAAACTCACTACAAT+GGG + chr5.1:5420794-5420813 None:intergenic 40.0%
GAAACTCAAGGAGCTTATGA+TGG - chr5.1:5420060-5420079 MS.gene21144:CDS 40.0%
GCAATACAAGAACATGGAAG+AGG - chr5.1:5420482-5420501 MS.gene21144:CDS 40.0%
GCCATTAGTAACAGCAATTC+TGG + chr5.1:5420032-5420051 None:intergenic 40.0%
!! GAAGCTGGTCTTCAAAAACA+AGG - chr5.1:5419686-5419705 MS.gene21144:CDS 40.0%
!! TGAAGCTTTGAAAGCACTGA+GGG - chr5.1:5419868-5419887 MS.gene21144:CDS 40.0%
ACATGGAAGAGGAAAGGTTC+TGG - chr5.1:5420493-5420512 MS.gene21144:CDS 45.0%
GCTGCTAATAGAAGACTCTC+TGG - chr5.1:5419974-5419993 MS.gene21144:CDS 45.0%
GCTGTTATGGTTGAAGCACA+AGG - chr5.1:5420605-5420624 MS.gene21144:CDS 45.0%
GGGAAGGCAATACAAGAACA+TGG - chr5.1:5420476-5420495 MS.gene21144:CDS 45.0%
! GGAGCTTATGATGGAGTTTC+AGG - chr5.1:5420069-5420088 MS.gene21144:intron 45.0%
! TGGCTTGCGTAAGAAACTCA+AGG - chr5.1:5420048-5420067 MS.gene21144:CDS 45.0%
!! AGGAAAGGTTCTGGAAACTG+TGG - chr5.1:5420502-5420521 MS.gene21144:CDS 45.0%
!! CTGAAGCTTTGAAAGCACTG+AGG - chr5.1:5419867-5419886 MS.gene21144:CDS 45.0%
!! GCTGGTCTTCAAAAACAAGG+TGG - chr5.1:5419689-5419708 MS.gene21144:CDS 45.0%
AGCAGGAAGTGGATGTGCAT+TGG - chr5.1:5420728-5420747 MS.gene21144:CDS 50.0%
GCAGGAAGTGGATGTGCATT+GGG - chr5.1:5420729-5420748 MS.gene21144:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 5419608 5420820 5419608 ID=MS.gene21144
chr5.1 mRNA 5419608 5420820 5419608 ID=MS.gene21144.t1;Parent=MS.gene21144
chr5.1 exon 5420338 5420820 5420338 ID=MS.gene21144.t1.exon1;Parent=MS.gene21144.t1
chr5.1 CDS 5420338 5420820 5420338 ID=cds.MS.gene21144.t1;Parent=MS.gene21144.t1
chr5.1 exon 5419608 5420084 5419608 ID=MS.gene21144.t1.exon2;Parent=MS.gene21144.t1
chr5.1 CDS 5419608 5420084 5419608 ID=cds.MS.gene21144.t1;Parent=MS.gene21144.t1
Gene Sequence

>MS.gene21144

ATGAATGATCTAATGACAAAATCATTCACCAACTATGTTGATCTAAAAAAAGCAGCTATGAAAGATGAAGTTGATTTAGAAGCTGGTCTTCAAAAACAAGGTGGTGTTGAATTAATAAGATCTTCACTGACCCATTTGGAAACTGATATGAATCTGTTCTTGGAAGAGGCAGAGAAAGTAAGAACAGAAATGGGTGAAATTCGCGATATACTAACAAAGCTTGAACAAGCAAATCAAGAGAGCAAGAGTCTTCACAAAACTGAAGCTTTGAAAGCACTGAGGGAAAGAATTAATACTGATATTGTAGCTGTTTTGAAGAAAGCAAAATGTATTAGGACTCAACTTGAAGAAATGGATCGTGCTAATGCTGCTAATAGAAGACTCTCTGGACTTAAAGATGGTTCACCAGCAATTTATAGAACCAGAATTGCTGTTACTAATGGCTTGCGTAAGAAACTCAAGGAGCTTATGATGGAGTTTCAGGTAGTTACCCTTGTCAATTTCTCTTCTGGTTTTTTTCTTTCTGGATTCAAATTAATATTTTTTTTTTGAATTTATGTATCAATTTTAAGGTTTAAAGTATGCATATTTGTGTAAGAAACTCATGGAATATGCATATCTGTGTGCCTTTTCTAAAAAGCATCATTGTTTTTTTTTCTTCTTCTAGTGTTAGTATGTTGTGTATCTTGTAACATTACAATCATCACAATTTATGAAACCTTGTATATTACAACAGGGACTTAGGCAGAAAATGATGAGTGAATACAAAGAAACAGTAGGGAGAAGGTATTATACAGTGACAGGGGAACATGCTGATGAAGAAGTAATTGAGAAGATAATTTCAAATGGAGATGATGAAAGTTTCTTAGGGAAGGCAATACAAGAACATGGAAGAGGAAAGGTTCTGGAAACTGTGGTAGAGATACAAGACAGACATGATGCTGCTAAAGAGATTGAGAAGAGTTTATTGGAACTTCATCAAATATTTTTGGATATGGCTGTTATGGTTGAAGCACAAGGTGAAAAAATGGATGATATTGAACATCATGTTCTTCATGCTTCTCATTATGTTAAAGATGGTACAAAGAATCTTCACGGTGCTAAACAGTATCAGAAAAGTAGCAGGAAGTGGATGTGCATTGGGATTATACTTTTGCTTATTCTGGTTCTCATCATTGTTATTCCCATTGTAGTGAGTTTCAGCAGTTCTTGA

Protein sequence

>MS.gene21144.t1

MNDLMTKSFTNYVDLKKAAMKDEVDLEAGLQKQGGVELIRSSLTHLETDMNLFLEEAEKVRTEMGEIRDILTKLEQANQESKSLHKTEALKALRERINTDIVAVLKKAKCIRTQLEEMDRANAANRRLSGLKDGSPAIYRTRIAVTNGLRKKLKELMMEFQGLRQKMMSEYKETVGRRYYTVTGEHADEEVIEKIISNGDDESFLGKAIQEHGRGKVLETVVEIQDRHDAAKEIEKSLLELHQIFLDMAVMVEAQGEKMDDIEHHVLHASHYVKDGTKNLHGAKQYQKSSRKWMCIGIILLLILVLIIVIPIVVSFSSS