AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003373

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003373.t1 MTR_4g076200 98.324 179 3 0 1 179 1 179 5.75e-132 367
MS.gene003373.t1 MTR_5g009020 85.311 177 26 0 1 177 1 177 1.31e-114 323
MS.gene003373.t1 MTR_4g076200 97.826 138 3 0 42 179 1 138 7.30e-101 286
MS.gene003373.t1 MTR_1g095510 66.871 163 53 1 18 179 27 189 3.30e-75 224
MS.gene003373.t1 MTR_1g095510 68.148 135 43 0 45 179 1 135 2.03e-64 194
MS.gene003373.t1 MTR_1g095510 60.123 163 44 2 18 179 27 169 9.53e-62 189
MS.gene003373.t1 MTR_4g108080 46.853 143 75 1 30 171 2 144 3.26e-40 134
MS.gene003373.t1 MTR_8g468530 45.517 145 78 1 30 173 18 162 1.09e-39 132
MS.gene003373.t1 MTR_2g078010 45.312 128 70 0 35 162 8 135 8.52e-35 119
MS.gene003373.t1 MTR_2g078010 45.312 128 70 0 35 162 8 135 1.15e-34 119
MS.gene003373.t1 MTR_8g071040 43.750 128 72 0 35 162 4 131 2.12e-34 119
MS.gene003373.t1 MTR_4g073240 44.715 123 68 0 35 157 4 126 7.62e-34 117
MS.gene003373.t1 MTR_3g062450 44.355 124 69 0 35 158 4 127 1.33e-33 116
MS.gene003373.t1 MTR_5g075580 44.355 124 69 0 35 158 4 127 1.48e-33 116
MS.gene003373.t1 MTR_7g116940 43.548 124 70 0 35 158 4 127 3.88e-33 115
MS.gene003373.t1 MTR_1g021150 42.969 128 73 0 35 162 4 131 9.23e-33 114
MS.gene003373.t1 MTR_2g078010 41.549 142 69 1 35 162 8 149 4.12e-32 113
MS.gene003373.t1 MTR_4g108080 48.624 109 56 0 30 138 2 110 1.45e-30 108
MS.gene003373.t1 MTR_1g021150 40.769 130 77 0 33 162 10 139 2.56e-30 108
MS.gene003373.t1 MTR_7g116940 41.221 131 70 1 35 158 4 134 4.37e-30 108
MS.gene003373.t1 MTR_4g073240 48.454 97 50 0 61 157 1 97 1.05e-29 105
MS.gene003373.t1 MTR_5g075580 47.959 98 51 0 61 158 1 98 2.13e-29 105
MS.gene003373.t1 MTR_4g108080 51.000 100 48 1 73 171 45 144 2.52e-28 103
MS.gene003373.t1 MTR_2g078010 45.545 101 55 0 35 135 8 108 6.49e-27 98.6
MS.gene003373.t1 MTR_4g063490 36.641 131 69 2 57 173 30 160 1.73e-26 99.4
MS.gene003373.t1 MTR_8g085690 38.710 124 76 0 35 158 25 148 4.30e-26 98.2
MS.gene003373.t1 MTR_7g116940 41.509 106 62 0 35 140 4 109 1.03e-25 95.9
MS.gene003373.t1 MTR_2g096690 36.641 131 69 2 57 173 30 160 1.27e-24 94.4
MS.gene003373.t1 MTR_2g096690 36.154 130 70 1 57 173 30 159 1.47e-24 94.4
MS.gene003373.t1 MTR_3g098780 37.778 135 80 3 36 168 6 138 6.19e-24 93.2
MS.gene003373.t1 MTR_3g098780 37.778 135 80 3 36 168 30 162 7.62e-24 93.6
MS.gene003373.t1 MTR_3g110110 38.393 112 68 1 47 157 20 131 3.27e-23 90.5
MS.gene003373.t1 MTR_4g094872 36.620 142 82 3 36 169 8 149 2.07e-21 85.9
MS.gene003373.t1 MTR_5g016630 35.616 146 86 3 36 173 8 153 2.51e-21 85.5
MS.gene003373.t1 MTR_4g106690 35.200 125 76 4 35 157 6 127 1.93e-19 81.3
MS.gene003373.t1 MTR_5g019480 38.144 97 57 1 73 169 21 114 5.71e-18 76.3
MS.gene003373.t1 MTR_5g019480 32.090 134 87 2 36 169 33 162 1.16e-17 76.6
MS.gene003373.t1 MTR_5g019480 38.144 97 57 1 73 169 72 165 1.20e-17 76.6
MS.gene003373.t1 MTR_8g028920 33.600 125 78 4 35 157 6 127 2.40e-17 75.9
MS.gene003373.t1 MTR_7g087590 30.968 155 99 2 18 171 3 150 3.36e-17 77.0
MS.gene003373.t1 MTR_4g073240 50.820 61 30 0 97 157 4 64 5.61e-16 69.7
MS.gene003373.t1 MTR_8g098595 36.667 90 55 2 70 157 23 112 1.78e-15 70.5
MS.gene003373.t1 MTR_8g076490 36.364 99 61 2 36 133 19 116 2.27e-15 70.1
MS.gene003373.t1 MTR_8g028920 36.905 84 51 2 76 157 5 88 9.26e-15 68.2
MS.gene003373.t1 MTR_5g021260 34.906 106 67 2 30 134 13 117 3.90e-14 67.0
MS.gene003373.t1 MTR_2g096690 44.615 65 36 0 57 121 30 94 8.28e-14 65.1
MS.gene003373.t1 MTR_4g121960 29.530 149 87 5 33 174 11 148 3.34e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003373.t1 AT3G20060 80.791 177 30 1 1 173 1 177 1.79e-101 290
MS.gene003373.t1 AT1G50490 81.111 180 31 1 1 177 1 180 3.12e-97 279
MS.gene003373.t1 AT3G20060 86.765 136 18 0 42 177 1 136 2.99e-87 252
MS.gene003373.t1 AT2G02760 46.154 143 76 1 30 171 2 144 1.78e-39 132
MS.gene003373.t1 AT2G02760 46.154 143 76 1 30 171 2 144 1.78e-39 132
MS.gene003373.t1 AT2G02760 46.154 143 76 1 30 171 2 144 1.78e-39 132
MS.gene003373.t1 AT2G02760 46.154 143 76 1 30 171 2 144 1.78e-39 132
MS.gene003373.t1 AT1G14400 46.154 143 76 1 30 171 2 144 1.88e-39 132
MS.gene003373.t1 AT1G14400 46.154 143 76 1 30 171 2 144 1.88e-39 132
MS.gene003373.t1 AT5G62540 46.479 142 74 2 30 169 2 143 5.27e-37 125
MS.gene003373.t1 AT1G78870 46.094 128 69 0 35 162 8 135 2.14e-35 121
MS.gene003373.t1 AT1G16890 45.312 128 70 0 35 162 8 135 7.77e-35 120
MS.gene003373.t1 AT5G56150 45.161 124 68 0 35 158 4 127 4.74e-34 118
MS.gene003373.t1 AT1G16890 46.923 130 67 1 35 162 15 144 5.11e-34 118
MS.gene003373.t1 AT1G64230 44.355 124 69 0 35 158 4 127 1.78e-33 116
MS.gene003373.t1 AT1G64230 44.355 124 69 0 35 158 4 127 1.78e-33 116
MS.gene003373.t1 AT2G16740 41.176 136 79 1 35 169 4 139 2.21e-33 116
MS.gene003373.t1 AT4G27960 41.860 129 75 0 35 163 4 132 2.44e-33 116
MS.gene003373.t1 AT3G08700 42.029 138 78 2 35 170 4 141 6.10e-33 115
MS.gene003373.t1 AT3G08700 42.029 138 78 2 35 170 4 141 6.10e-33 115
MS.gene003373.t1 AT3G08690 43.548 124 70 0 35 158 4 127 6.54e-33 115
MS.gene003373.t1 AT3G08690 43.548 124 70 0 35 158 4 127 6.54e-33 115
MS.gene003373.t1 AT5G53300 41.085 129 76 0 35 163 4 132 1.08e-32 114
MS.gene003373.t1 AT5G53300 41.085 129 76 0 35 163 4 132 1.08e-32 114
MS.gene003373.t1 AT5G53300 41.085 129 76 0 35 163 4 132 1.08e-32 114
MS.gene003373.t1 AT5G41700 42.742 124 71 0 35 158 4 127 1.41e-32 114
MS.gene003373.t1 AT5G41700 42.742 124 71 0 35 158 4 127 1.41e-32 114
MS.gene003373.t1 AT5G41700 42.742 124 71 0 35 158 4 127 1.41e-32 114
MS.gene003373.t1 AT4G27960 41.860 129 75 0 35 163 34 162 3.94e-32 114
MS.gene003373.t1 AT5G41700 43.200 125 70 1 35 158 4 128 6.79e-31 110
MS.gene003373.t1 AT1G64230 41.353 133 69 1 35 158 4 136 1.91e-30 108
MS.gene003373.t1 AT1G64230 46.602 103 55 0 56 158 67 169 1.27e-29 107
MS.gene003373.t1 AT1G64230 46.602 103 55 0 56 158 67 169 1.27e-29 107
MS.gene003373.t1 AT1G16890 50.526 95 47 0 68 162 8 102 4.65e-29 104
MS.gene003373.t1 AT1G78870 50.526 95 47 0 68 162 8 102 5.07e-29 104
MS.gene003373.t1 AT1G78870 45.714 105 57 0 35 139 8 112 5.68e-28 101
MS.gene003373.t1 AT1G78870 50.000 96 47 1 68 162 8 103 1.26e-27 100
MS.gene003373.t1 AT3G13550 37.500 136 85 0 35 170 39 174 6.05e-27 100
MS.gene003373.t1 AT1G36340 40.876 137 79 2 26 162 3 137 5.91e-26 97.4
MS.gene003373.t1 AT1G36340 40.876 137 79 2 26 162 3 137 5.91e-26 97.4
MS.gene003373.t1 AT5G50870 41.935 124 67 3 34 155 4 124 1.08e-25 97.8
MS.gene003373.t1 AT5G50870 41.935 124 67 3 34 155 4 124 1.36e-25 97.8
MS.gene003373.t1 AT5G56150 43.000 100 57 0 35 134 4 103 1.73e-25 95.1
MS.gene003373.t1 AT5G41700 40.385 104 62 0 35 138 4 107 2.47e-24 93.2
MS.gene003373.t1 AT3G13550 39.640 111 67 0 35 145 39 149 3.51e-24 93.2
MS.gene003373.t1 AT3G55380 35.115 131 71 2 57 173 31 161 8.54e-24 92.4
MS.gene003373.t1 AT5G53300 40.404 99 59 0 35 133 4 102 9.48e-24 90.5
MS.gene003373.t1 AT5G59300 35.115 131 71 2 57 173 30 160 9.57e-24 92.0
MS.gene003373.t1 AT3G46460 35.878 131 70 2 57 173 30 160 1.65e-23 91.7
MS.gene003373.t1 AT5G25760 38.739 111 67 1 48 157 21 131 3.35e-23 90.5
MS.gene003373.t1 AT5G25760 38.739 111 67 1 48 157 21 131 3.35e-23 90.5
MS.gene003373.t1 AT1G63800 37.600 125 73 4 35 157 6 127 5.72e-21 85.5
MS.gene003373.t1 AT3G57870 36.620 142 82 3 36 169 9 150 1.57e-20 83.6
MS.gene003373.t1 AT5G41340 36.800 125 74 4 35 157 6 127 2.09e-20 84.0
MS.gene003373.t1 AT2G18600 34.959 123 77 3 36 157 35 155 5.70e-19 80.1
MS.gene003373.t1 AT2G18600 34.959 123 77 3 36 157 35 155 5.70e-19 80.1
MS.gene003373.t1 AT1G63800 40.217 92 53 2 68 157 21 112 8.45e-19 79.3
MS.gene003373.t1 AT2G46030 35.200 125 76 4 35 157 6 127 1.30e-18 79.3
MS.gene003373.t1 AT2G46030 35.200 125 76 4 35 157 6 127 1.30e-18 79.3
MS.gene003373.t1 AT5G05080 33.099 142 93 2 32 172 10 150 7.16e-18 78.6
MS.gene003373.t1 AT5G05080 33.099 142 93 2 32 172 10 150 7.16e-18 78.6
MS.gene003373.t1 AT5G05080 33.099 142 93 2 32 172 10 150 7.16e-18 78.6
MS.gene003373.t1 AT5G05080 33.099 142 93 2 32 172 10 150 7.16e-18 78.6
MS.gene003373.t1 AT2G46030 35.200 125 76 4 35 157 78 199 9.15e-18 78.6
MS.gene003373.t1 AT4G36800 31.148 122 83 1 36 157 34 154 5.43e-17 75.1
MS.gene003373.t1 AT4G36800 31.148 122 83 1 36 157 34 154 5.43e-17 75.1
MS.gene003373.t1 AT3G55380 27.907 172 75 4 51 173 24 195 1.99e-16 73.9
MS.gene003373.t1 AT3G46460 33.333 111 60 2 77 173 8 118 5.28e-16 70.9
MS.gene003373.t1 AT3G46460 33.333 111 60 2 77 173 8 118 5.28e-16 70.9
MS.gene003373.t1 AT2G46030 35.644 101 63 2 59 157 30 130 7.38e-16 72.0
MS.gene003373.t1 AT2G46030 35.644 101 63 2 59 157 30 130 7.38e-16 72.0
MS.gene003373.t1 AT5G41340 40.000 85 49 2 75 157 8 92 8.25e-16 71.2
MS.gene003373.t1 AT3G24515 37.391 115 63 4 68 173 51 165 9.78e-16 74.3
MS.gene003373.t1 AT3G24515 37.391 115 63 4 68 173 76 190 1.41e-15 73.9
MS.gene003373.t1 AT1G45050 33.929 112 72 2 24 134 7 117 6.38e-15 68.9
MS.gene003373.t1 AT1G75440 34.234 111 71 2 24 133 7 116 2.66e-14 67.4
MS.gene003373.t1 AT5G42990 33.929 112 72 2 24 134 7 117 2.80e-14 67.4
MS.gene003373.t1 AT4G36410 35.354 99 62 2 36 133 19 116 3.88e-14 67.0
MS.gene003373.t1 AT2G32790 38.017 121 72 2 53 173 51 168 4.71e-14 67.0
MS.gene003373.t1 AT3G17000 32.743 113 67 3 34 141 13 121 7.09e-12 62.8

Find 41 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACTTTAGGAGCCTTAAA+AGG 0.184751 4.1:+28070513 None:intergenic
TTGTTTGTTTATAGGATAAA+TGG 0.261888 4.1:-28070341 MS.gene003373:intron
CATCCCAATGTTGATCTTCA+TGG 0.269716 4.1:-28070471 MS.gene003373:CDS
GCCTTCAAACACTGTATCTT+TGG 0.311394 4.1:+28070575 None:intergenic
CTGTCCATCCAAAGTCTTCT+TGG 0.336173 4.1:-28070288 MS.gene003373:CDS
TGTCAGATGAGCGGTGATTC+TGG 0.340319 4.1:-28070657 MS.gene003373:intron
AGCATCAGTGTTCCCATTGA+TGG 0.378850 4.1:+28071893 None:intergenic
GTAGTTTCAAACTTGACTTT+AGG 0.379535 4.1:+28070501 None:intergenic
TTGTGGAAAGGAACAATAAC+AGG 0.394846 4.1:-28070600 MS.gene003373:CDS
ATCCACAAGCAGCACAGCTT+TGG 0.411990 4.1:-28070052 MS.gene003373:CDS
GCCTTAAAAGGGTAATCATT+AGG 0.415557 4.1:+28070525 None:intergenic
TATATGCTTGGACATACTTC+AGG 0.420859 4.1:-28070445 MS.gene003373:intron
GTCTGAATTGATGTCTTTGA+TGG 0.426742 4.1:-28070901 MS.gene003373:intron
CTCCAAAGCTGTGCTGCTTG+TGG 0.432715 4.1:+28070050 None:intergenic
ATTCCACCTCTGTTCTCAAA+AGG 0.440278 4.1:-28071815 MS.gene003373:intron
TGTGCTGCTTGTGGATTTAG+TGG 0.440911 4.1:+28070059 None:intergenic
TTTAGTGGTGAACTAATGTT+TGG 0.452232 4.1:+28070074 None:intergenic
GTCTACCTCCAAGAAGACTT+TGG 0.472732 4.1:+28070280 None:intergenic
AAGAATATGTTGTCTTCCTC+AGG 0.473205 4.1:+28070621 None:intergenic
TGACTTTAGGAGCCTTAAAA+GGG 0.487045 4.1:+28070514 None:intergenic
ATCAACATTGGGATGGAAGC+AGG 0.492019 4.1:+28070479 None:intergenic
TTATACAGATTACAGAAAGA+TGG 0.493470 4.1:-28069931 MS.gene003373:intron
TTGCCATGAAGATCAACATT+GGG 0.507321 4.1:+28070468 None:intergenic
TCTTCATGGCAATATATGCT+TGG 0.513262 4.1:-28070457 MS.gene003373:CDS
GAGGTGGAATCAACGGACTT+AGG 0.525977 4.1:+28071828 None:intergenic
ATCGACTAAGCAGTAGCACT+TGG 0.531003 4.1:+28069888 None:intergenic
ACCTCCAAGAAGACTTTGGA+TGG 0.537043 4.1:+28070284 None:intergenic
AGATACAGTGTTTGAAGGCA+CGG 0.542633 4.1:-28070571 MS.gene003373:CDS
TCCATCCAAAGTCTTCTTGG+AGG 0.548893 4.1:-28070285 MS.gene003373:intron
ACCAAAGATACAGTGTTTGA+AGG 0.558474 4.1:-28070576 MS.gene003373:CDS
GACAACATATTCTTGTGGAA+AGG 0.561762 4.1:-28070612 MS.gene003373:CDS
ACGGACTTAGGAGGAGGAAG+AGG 0.570689 4.1:+28071840 None:intergenic
GAGAACAGAGGTGGAATCAA+CGG 0.579252 4.1:+28071821 None:intergenic
ATTGCCATGAAGATCAACAT+TGG 0.588583 4.1:+28070467 None:intergenic
GAATCAACGGACTTAGGAGG+AGG 0.597125 4.1:+28071834 None:intergenic
AGGAAGACAACATATTCTTG+TGG 0.600574 4.1:-28070617 MS.gene003373:CDS
GTGGAATCAACGGACTTAGG+AGG 0.610762 4.1:+28071831 None:intergenic
CAGCTTTGGAGCAATCAAGA+AGG 0.619795 4.1:-28070038 MS.gene003373:intron
TACAGATTACAGAAAGATGG+TGG 0.634223 4.1:-28069928 MS.gene003373:intron
CATGAAGATCAACATTGGGA+TGG 0.643434 4.1:+28070472 None:intergenic
TGATTGGACTGTCAGATGAG+CGG 0.704407 4.1:-28070666 MS.gene003373:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAATGTATAAATAAA+GGG - chr4.1:28070425-28070444 MS.gene003373:intron 10.0%
!! TAAGAAAAATGTATAAATAA+AGG - chr4.1:28070424-28070443 MS.gene003373:intron 10.0%
!! AATTAGAGTTATATTGTATT+GGG - chr4.1:28070490-28070509 MS.gene003373:CDS 15.0%
!! ACAAATGACAATAATAATAA+GGG + chr4.1:28070780-28070799 None:intergenic 15.0%
!! AGAAAAATGTATAAATAAAG+GGG - chr4.1:28070426-28070445 MS.gene003373:intron 15.0%
!! TAAAAAGTTAAAAAAGAGTT+AGG + chr4.1:28071688-28071707 None:intergenic 15.0%
!! TAAAAGAGAGATTTAATAAT+AGG + chr4.1:28070015-28070034 None:intergenic 15.0%
!! TAATTAGAGTTATATTGTAT+TGG - chr4.1:28070489-28070508 MS.gene003373:CDS 15.0%
!! TACAAATGACAATAATAATA+AGG + chr4.1:28070781-28070800 None:intergenic 15.0%
!! TTATTTGTTTGTTTGTTTAT+AGG - chr4.1:28071444-28071463 MS.gene003373:intron 15.0%
!! TTGTTTGTTTATAGGATAAA+TGG - chr4.1:28071452-28071471 MS.gene003373:intron 20.0%
!!! ACACTAATCTAATTTGTATA+CGG + chr4.1:28071650-28071669 None:intergenic 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG - chr4.1:28070559-28070578 MS.gene003373:CDS 20.0%
! GAACAAATGATTGTGATAAA+TGG - chr4.1:28070312-28070331 MS.gene003373:CDS 25.0%
! TTATACAGATTACAGAAAGA+TGG - chr4.1:28071862-28071881 MS.gene003373:CDS 25.0%
!! TTGAAATGACTCAGTTATTT+TGG - chr4.1:28070536-28070555 MS.gene003373:CDS 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG - chr4.1:28070558-28070577 MS.gene003373:CDS 25.0%
!!! TGAAATGACTCAGTTATTTT+GGG - chr4.1:28070537-28070556 MS.gene003373:CDS 25.0%
!!! TGTTTTGCATTTTTTGTGAT+TGG - chr4.1:28071111-28071130 MS.gene003373:intron 25.0%
ACAAGACTCATAATGATTGA+AGG + chr4.1:28071788-28071807 None:intergenic 30.0%
AGGTAATGTGAAATCATCTA+AGG - chr4.1:28071368-28071387 MS.gene003373:intron 30.0%
ATGTTAATGTATGATGTCGT+CGG + chr4.1:28070142-28070161 None:intergenic 30.0%
CATACATTAACATGATCAGT+GGG - chr4.1:28070149-28070168 MS.gene003373:intron 30.0%
CATGTATTTAGCTAATAGTG+TGG - chr4.1:28071064-28071083 MS.gene003373:intron 30.0%
GTAGTTTCAAACTTGACTTT+AGG + chr4.1:28071295-28071314 None:intergenic 30.0%
TCATACATTAACATGATCAG+TGG - chr4.1:28070148-28070167 MS.gene003373:intron 30.0%
TCTGAACCTCAACAAAATTA+AGG + chr4.1:28071006-28071025 None:intergenic 30.0%
TGAAAAAACTAAGCTACACA+AGG + chr4.1:28070705-28070724 None:intergenic 30.0%
TGTATAAATAAAGGGGAAAG+TGG - chr4.1:28070433-28070452 MS.gene003373:intron 30.0%
TTTAGTGGTGAACTAATGTT+TGG + chr4.1:28071722-28071741 None:intergenic 30.0%
! AATCATTATGAGTCTTGTGA+TGG - chr4.1:28071790-28071809 MS.gene003373:intron 30.0%
! TCCTAATGATTACCCTTTTA+AGG - chr4.1:28071267-28071286 MS.gene003373:intron 30.0%
!! TAGGAGTACTAGTTAAGAAA+TGG + chr4.1:28070742-28070761 None:intergenic 30.0%
!!! ATTTGTTGTTGACCTTGTTT+TGG - chr4.1:28071406-28071425 MS.gene003373:intron 30.0%
!!! CACGTTCATTATTGTTTTTG+AGG - chr4.1:28070955-28070974 MS.gene003373:intron 30.0%
AAGAATATGTTGTCTTCCTC+AGG + chr4.1:28071175-28071194 None:intergenic 35.0%
ACAATGTGCGAATGTAACTT+TGG + chr4.1:28071036-28071055 None:intergenic 35.0%
ACCAAAGATACAGTGTTTGA+AGG - chr4.1:28071217-28071236 MS.gene003373:intron 35.0%
ACTACAAGTGTCTAAGTAAG+TGG - chr4.1:28070637-28070656 MS.gene003373:CDS 35.0%
AGAAAATGGGTCAGATATTC+CGG - chr4.1:28070572-28070591 MS.gene003373:CDS 35.0%
AGGAAGACAACATATTCTTG+TGG - chr4.1:28071176-28071195 MS.gene003373:intron 35.0%
ATCTGTGTGAAAATCCAAAC+TGG + chr4.1:28070404-28070423 None:intergenic 35.0%
ATGTAGCCGTAACACATAAA+TGG + chr4.1:28070667-28070686 None:intergenic 35.0%
ATTGCCATGAAGATCAACAT+TGG + chr4.1:28071329-28071348 None:intergenic 35.0%
CTACAAGTGTCTAAGTAAGT+GGG - chr4.1:28070638-28070657 MS.gene003373:CDS 35.0%
CTTGTATGCATTCTTAGATG+AGG - chr4.1:28070182-28070201 MS.gene003373:intron 35.0%
GACAACATATTCTTGTGGAA+AGG - chr4.1:28071181-28071200 MS.gene003373:intron 35.0%
GCCTTAAAAGGGTAATCATT+AGG + chr4.1:28071271-28071290 None:intergenic 35.0%
GCTCAACACACAAATCAAAT+TGG + chr4.1:28070864-28070883 None:intergenic 35.0%
GTCTGAATTGATGTCTTTGA+TGG - chr4.1:28070892-28070911 MS.gene003373:intron 35.0%
TACAGATTACAGAAAGATGG+TGG - chr4.1:28071865-28071884 MS.gene003373:CDS 35.0%
TATATGCTTGGACATACTTC+AGG - chr4.1:28071348-28071367 MS.gene003373:intron 35.0%
TCTTCATGGCAATATATGCT+TGG - chr4.1:28071336-28071355 MS.gene003373:intron 35.0%
TTGCCATGAAGATCAACATT+GGG + chr4.1:28071328-28071347 None:intergenic 35.0%
TTGTGGAAAGGAACAATAAC+AGG - chr4.1:28071193-28071212 MS.gene003373:intron 35.0%
! TGACTTTAGGAGCCTTAAAA+GGG + chr4.1:28071282-28071301 None:intergenic 35.0%
! TTGACTTTAGGAGCCTTAAA+AGG + chr4.1:28071283-28071302 None:intergenic 35.0%
!! TGTTCGCTTTTATGTCACTT+TGG + chr4.1:28070297-28070316 None:intergenic 35.0%
!! TGTTTTGTTGGAGCAACAAT+GGG + chr4.1:28069932-28069951 None:intergenic 35.0%
!! TTGTTTTGTTGGAGCAACAA+TGG + chr4.1:28069933-28069952 None:intergenic 35.0%
!!! ACACGTTCTTTGTTTTGTGT+TGG - chr4.1:28070837-28070856 MS.gene003373:intron 35.0%
ACACCTTATACCGTTCATGT+GGG - chr4.1:28070255-28070274 MS.gene003373:intron 40.0%
ACATGAACGGTATAAGGTGT+CGG + chr4.1:28070255-28070274 None:intergenic 40.0%
ACTTGTGTGATCCACATCAA+TGG - chr4.1:28070205-28070224 MS.gene003373:intron 40.0%
AGATACAGTGTTTGAAGGCA+CGG - chr4.1:28071222-28071241 MS.gene003373:intron 40.0%
ATTCCACCTCTGTTCTCAAA+AGG - chr4.1:28069978-28069997 MS.gene003373:intron 40.0%
CATCCCAATGTTGATCTTCA+TGG - chr4.1:28071322-28071341 MS.gene003373:intron 40.0%
CATGAAGATCAACATTGGGA+TGG + chr4.1:28071324-28071343 None:intergenic 40.0%
GAAAATGGGTCAGATATTCC+GGG - chr4.1:28070573-28070592 MS.gene003373:CDS 40.0%
GCCTTCAAACACTGTATCTT+TGG + chr4.1:28071221-28071240 None:intergenic 40.0%
GGGCATCCATTTATGTGTTA+CGG - chr4.1:28070658-28070677 MS.gene003373:intron 40.0%
! CAAACTGGCTCAGATAAAAC+GGG + chr4.1:28070389-28070408 None:intergenic 40.0%
! CCAAACTGGCTCAGATAAAA+CGG + chr4.1:28070390-28070409 None:intergenic 40.0%
! GTGACGCCTTAATTTTGTTG+AGG - chr4.1:28070997-28071016 MS.gene003373:intron 40.0%
!! AAACCTTTTGAGAACAGAGG+TGG + chr4.1:28069984-28070003 None:intergenic 40.0%
!! AGGGTTTGCTAACTAGTGTT+AGG + chr4.1:28070761-28070780 None:intergenic 40.0%
!! GAGAAACCTTTTGAGAACAG+AGG + chr4.1:28069987-28070006 None:intergenic 40.0%
!! TGGTATATCTGCTTTTCCTG+AGG - chr4.1:28071156-28071175 MS.gene003373:intron 40.0%
!!! TTGTTGACCTTGTTTTGGCT+TGG - chr4.1:28071411-28071430 MS.gene003373:intron 40.0%
ACCTCCAAGAAGACTTTGGA+TGG + chr4.1:28071512-28071531 None:intergenic 45.0%
AGCATCAGTGTTCCCATTGA+TGG + chr4.1:28069903-28069922 None:intergenic 45.0%
ATCAACATTGGGATGGAAGC+AGG + chr4.1:28071317-28071336 None:intergenic 45.0%
ATTAAATACTCCCTCCGTCC+CGG + chr4.1:28070594-28070613 None:intergenic 45.0%
CAGTATGCCAAGCCAAAACA+AGG + chr4.1:28071421-28071440 None:intergenic 45.0%
CATGATCAGTGGGTCTTTGT+TGG - chr4.1:28070159-28070178 MS.gene003373:intron 45.0%
CTGTCCATCCAAAGTCTTCT+TGG - chr4.1:28071505-28071524 MS.gene003373:intron 45.0%
GACACCTTATACCGTTCATG+TGG - chr4.1:28070254-28070273 MS.gene003373:intron 45.0%
GAGAACAGAGGTGGAATCAA+CGG + chr4.1:28069975-28069994 None:intergenic 45.0%
GTCTACCTCCAAGAAGACTT+TGG + chr4.1:28071516-28071535 None:intergenic 45.0%
TACCGTTCATGTGGGTCATT+CGG - chr4.1:28070263-28070282 MS.gene003373:intron 45.0%
TCCATCCAAAGTCTTCTTGG+AGG - chr4.1:28071508-28071527 MS.gene003373:intron 45.0%
TGACCCACATGAACGGTATA+AGG + chr4.1:28070261-28070280 None:intergenic 45.0%
TGATCCACATCAATGGCACA+CGG - chr4.1:28070212-28070231 MS.gene003373:intron 45.0%
TGATTGGACTGTCAGATGAG+CGG - chr4.1:28071127-28071146 MS.gene003373:intron 45.0%
! CCGTTTTATCTGAGCCAGTT+TGG - chr4.1:28070387-28070406 MS.gene003373:intron 45.0%
!! CAGCTTTGGAGCAATCAAGA+AGG - chr4.1:28071755-28071774 MS.gene003373:intron 45.0%
!! GGAGGAAGAGGTTGTTTTGT+TGG + chr4.1:28069944-28069963 None:intergenic 45.0%
!! TGTGCTGCTTGTGGATTTAG+TGG + chr4.1:28071737-28071756 None:intergenic 45.0%
ACCCGAATGACCCACATGAA+CGG + chr4.1:28070268-28070287 None:intergenic 50.0%
ACCGTTCATGTGGGTCATTC+GGG - chr4.1:28070264-28070283 MS.gene003373:intron 50.0%
ATCCACAAGCAGCACAGCTT+TGG - chr4.1:28071741-28071760 MS.gene003373:intron 50.0%
ATGGGTCAGATATTCCGGGA+CGG - chr4.1:28070577-28070596 MS.gene003373:CDS 50.0%
CTCAGATAAAACGGGACGGA+GGG + chr4.1:28070381-28070400 None:intergenic 50.0%
CTGGCTCAGATAAAACGGGA+CGG + chr4.1:28070385-28070404 None:intergenic 50.0%
TGTCAGATGAGCGGTGATTC+TGG - chr4.1:28071136-28071155 MS.gene003373:intron 50.0%
! GAATCAACGGACTTAGGAGG+AGG + chr4.1:28069962-28069981 None:intergenic 50.0%
! GAGGTGGAATCAACGGACTT+AGG + chr4.1:28069968-28069987 None:intergenic 50.0%
! GTGGAATCAACGGACTTAGG+AGG + chr4.1:28069965-28069984 None:intergenic 50.0%
ACGGACTTAGGAGGAGGAAG+AGG + chr4.1:28069956-28069975 None:intergenic 55.0%
GCTCAGATAAAACGGGACGG+AGG + chr4.1:28070382-28070401 None:intergenic 55.0%
GTCAGATATTCCGGGACGGA+GGG - chr4.1:28070581-28070600 MS.gene003373:CDS 55.0%
! GACACCGTGTGCCATTGATG+TGG + chr4.1:28070219-28070238 None:intergenic 55.0%
!! CTCCAAAGCTGTGCTGCTTG+TGG + chr4.1:28071746-28071765 None:intergenic 55.0%
GGTCAGATATTCCGGGACGG+AGG - chr4.1:28070580-28070599 MS.gene003373:CDS 60.0%
!! AAGGTGTCGGTGCACTCTGC+AGG + chr4.1:28070242-28070261 None:intergenic 60.0%
!! AGGTGTCGGTGCACTCTGCA+GGG + chr4.1:28070241-28070260 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 28069893 28071922 28069893 ID=MS.gene003373
chr4.1 mRNA 28069893 28071922 28069893 ID=MS.gene003373.t1;Parent=MS.gene003373
chr4.1 exon 28071816 28071922 28071816 ID=MS.gene003373.t1.exon1;Parent=MS.gene003373.t1
chr4.1 CDS 28071816 28071922 28071816 ID=cds.MS.gene003373.t1;Parent=MS.gene003373.t1
chr4.1 exon 28070902 28070929 28070902 ID=MS.gene003373.t1.exon2;Parent=MS.gene003373.t1
chr4.1 CDS 28070902 28070929 28070902 ID=cds.MS.gene003373.t1;Parent=MS.gene003373.t1
chr4.1 exon 28070446 28070673 28070446 ID=MS.gene003373.t1.exon3;Parent=MS.gene003373.t1
chr4.1 CDS 28070446 28070673 28070446 ID=cds.MS.gene003373.t1;Parent=MS.gene003373.t1
chr4.1 exon 28070286 28070349 28070286 ID=MS.gene003373.t1.exon4;Parent=MS.gene003373.t1
chr4.1 CDS 28070286 28070349 28070286 ID=cds.MS.gene003373.t1;Parent=MS.gene003373.t1
chr4.1 exon 28070039 28070098 28070039 ID=MS.gene003373.t1.exon5;Parent=MS.gene003373.t1
chr4.1 CDS 28070039 28070098 28070039 ID=cds.MS.gene003373.t1;Parent=MS.gene003373.t1
chr4.1 exon 28069893 28069945 28069893 ID=MS.gene003373.t1.exon6;Parent=MS.gene003373.t1
chr4.1 CDS 28069893 28069945 28069893 ID=cds.MS.gene003373.t1;Parent=MS.gene003373.t1
Gene Sequence

>MS.gene003373

ATGGCTACCATCAATGGGAACACTGATGCTGCTCAACCCATTGTTGCTCCAACAAAACAACCTCTTCCTCCTCCTAAGTCCGTTGATTCCACCTCTGTTCTCAAAAGGTTTCTCATTCACCTATTATTAAATCTCTCTTTTACTTGTTTTTGTTTCATGTCAATATGTGTTAACTTGTGTTTTTTCAATCGCTTCATCACACTTATGTTCTTTTTAAAATAATGTATGCTGACATTCAAAGTAATACCGACGACATCATACATTAACATGATCAGTGGGTCTTTGTTGGCTTGTATGCATTCTTAGATGAGGACTTGTGTGATCCACATCAATGGCACACGGTGTCCCTGCAGAGTGCACCGACACCTTATACCGTTCATGTGGGTCATTCGGGTCAGTTTCCAAAGTGACATAAAAGCGAACAAATGATTGTGATAAATGGTGTCGATATCACTTTGAGTAATAGCACAACATTATTGTATACTCCCTCCGTCCCGTTTTATCTGAGCCAGTTTGGATTTTCACACAGATTAAGAAAAATGTATAAATAAAGGGGAAAGTGGAATATATTGTACTGAGAATGATGAAAGTAGTATTAATTAGAGTTATATTGTATTGGGAATGATGAAAGTTGTATTGAAAATTGAAATGACTCAGTTATTTTGGGACACTTTTTTTTAGAAAATGGGTCAGATATTCCGGGACGGAGGGAGTATTTAATTAGTTTCATTATTTTCTTAAATTACTACAAGTGTCTAAGTAAGTGGGCATCCATTTATGTGTTACGGCTACATAGATCAACTGTGATGCCTTGTGTAGCTTAGTTTTTTCAAATCAATGAGTTAACCATTTCTTAACTAGTACTCCTAACACTAGTTAGCAAACCCTTATTATTATTGTCATTTGTAACAGCATTTGATTTGATTTGATTTGATTTGATTCTAACACGTTCTTTGTTTTGTGTTGGTCCAATTTGATTTGTGTGTTGAGCAGATTGCAGTCTGAATTGATGTCTTTGATGGTAAGCTTATATCAACTATCATTAATCATTATTGATTCTAACACGTTCATTATTGTTTTTGAGGCTGCTTGTTTAGAATCACAGTGACGCCTTAATTTTGTTGAGGTTCAGAGTTTTTGCCAAAGTTACATTCGCACATTGTGAATCAAACATGTATTTAGCTAATAGTGTGGATCAGTTGAAAACATAGATATTAATGTTTTGCATTTTTTGTGATTGGACTGTCAGATGAGCGGTGATTCTGGTATATCTGCTTTTCCTGAGGAAGACAACATATTCTTGTGGAAAGGAACAATAACAGGAACCAAAGATACAGTGTTTGAAGGCACGGATTACAAGTTATCACTTTCTTTTCCTAATGATTACCCTTTTAAGGCTCCTAAAGTCAAGTTTGAAACTACCTGCTTCCATCCCAATGTTGATCTTCATGGCAATATATGCTTGGACATACTTCAGGTAATGTGAAATCATCTAAGGAACATTTTAATGAGCATTTGTTGTTGACCTTGTTTTGGCTTGGCATACTGATCTTATTTGTTTGTTTGTTTATAGGATAAATGGTCATCTGCTTATGATGTCAGAACAATTCTACTGTCCATCCAAAGTCTTCTTGGAGGTAGACACAATGATTGAAAATTTTGTGCACACTTTAATTAATTGTTTTCTGAAATTTATATGCTGTTAACAAGTTTTTCATATGCAGATATATGTGTAAAACTATTTTAAGCTTGCACCGTATACAAATTAGATTAGTGTATTCTTTGTACTTTTCCTAACTCTTTTTTAACTTTTTAATAATGTAGAGCCAAACATTAGTTCACCACTAAATCCACAAGCAGCACAGCTTTGGAGCAATCAAGAAGGTAATAGTCCTTCAATCATTATGAGTCTTGTGATGGATATAATTTGTGAATCTCAAATATAGTTATAATGATTTTTGATGATTTGTTATACAGATTACAGAAAGATGGTGGAGAAGTTGTACAAAGCTCCAAGTGCTACTGCTTAG
Protein sequence

>MS.gene003373.t1

MATINGNTDAAQPIVAPTKQPLPPPKSVDSTSVLKRLQSELMSLMMSGDSGISAFPEEDNIFLWKGTITGTKDTVFEGTDYKLSLSFPNDYPFKAPKVKFETTCFHPNVDLHGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNPQAAQLWSNQEDYRKMVEKLYKAPSATA