AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031830.t1 MTR_4g076200 98.883 179 2 0 1 179 1 179 1.61e-132 368
MS.gene031830.t1 MTR_5g009020 84.746 177 27 0 1 177 1 177 6.15e-114 321
MS.gene031830.t1 MTR_4g076200 98.551 138 2 0 42 179 1 138 1.79e-101 288
MS.gene031830.t1 MTR_1g095510 66.258 163 54 1 18 179 27 189 1.70e-74 222
MS.gene031830.t1 MTR_1g095510 67.407 135 44 0 45 179 1 135 1.05e-63 192
MS.gene031830.t1 MTR_1g095510 59.509 163 45 2 18 179 27 169 4.35e-61 187
MS.gene031830.t1 MTR_4g108080 46.853 143 75 1 30 171 2 144 2.15e-40 134
MS.gene031830.t1 MTR_8g468530 45.517 145 78 1 30 173 18 162 8.70e-40 133
MS.gene031830.t1 MTR_2g078010 45.312 128 70 0 35 162 8 135 7.09e-35 120
MS.gene031830.t1 MTR_2g078010 45.312 128 70 0 35 162 8 135 9.42e-35 120
MS.gene031830.t1 MTR_8g071040 43.750 128 72 0 35 162 4 131 1.55e-34 119
MS.gene031830.t1 MTR_4g073240 44.715 123 68 0 35 157 4 126 6.07e-34 117
MS.gene031830.t1 MTR_3g062450 44.355 124 69 0 35 158 4 127 1.02e-33 117
MS.gene031830.t1 MTR_5g075580 44.355 124 69 0 35 158 4 127 1.14e-33 117
MS.gene031830.t1 MTR_7g116940 43.548 124 70 0 35 158 4 127 2.77e-33 115
MS.gene031830.t1 MTR_1g021150 42.969 128 73 0 35 162 4 131 5.85e-33 115
MS.gene031830.t1 MTR_2g078010 41.549 142 69 1 35 162 8 149 3.39e-32 114
MS.gene031830.t1 MTR_4g108080 48.624 109 56 0 30 138 2 110 9.73e-31 108
MS.gene031830.t1 MTR_1g021150 40.769 130 77 0 33 162 10 139 2.35e-30 108
MS.gene031830.t1 MTR_7g116940 41.221 131 70 1 35 158 4 134 3.12e-30 108
MS.gene031830.t1 MTR_4g073240 48.454 97 50 0 61 157 1 97 9.98e-30 105
MS.gene031830.t1 MTR_5g075580 47.959 98 51 0 61 158 1 98 1.77e-29 105
MS.gene031830.t1 MTR_4g108080 51.000 100 48 1 73 171 45 144 2.49e-28 103
MS.gene031830.t1 MTR_2g078010 45.545 101 55 0 35 135 8 108 5.89e-27 98.6
MS.gene031830.t1 MTR_4g063490 36.641 131 69 2 57 173 30 160 2.03e-26 99.0
MS.gene031830.t1 MTR_8g085690 38.710 124 76 0 35 158 25 148 3.32e-26 98.6
MS.gene031830.t1 MTR_7g116940 41.509 106 62 0 35 140 4 109 8.27e-26 96.3
MS.gene031830.t1 MTR_2g096690 36.641 131 69 2 57 173 30 160 1.22e-24 94.4
MS.gene031830.t1 MTR_3g098780 38.519 135 79 3 36 168 6 138 1.32e-24 95.1
MS.gene031830.t1 MTR_2g096690 36.154 130 70 1 57 173 30 159 1.42e-24 94.4
MS.gene031830.t1 MTR_3g098780 38.519 135 79 3 36 168 30 162 1.98e-24 95.1
MS.gene031830.t1 MTR_3g110110 40.196 102 60 1 57 157 30 131 1.37e-22 89.0
MS.gene031830.t1 MTR_4g094872 36.620 142 82 3 36 169 8 149 1.94e-21 85.9
MS.gene031830.t1 MTR_5g016630 35.616 146 86 3 36 173 8 153 2.43e-21 85.5
MS.gene031830.t1 MTR_4g106690 34.400 125 77 3 35 157 6 127 4.36e-19 80.5
MS.gene031830.t1 MTR_5g019480 32.090 134 87 2 36 169 33 162 5.62e-18 77.4
MS.gene031830.t1 MTR_5g019480 38.144 97 57 1 73 169 21 114 5.90e-18 76.3
MS.gene031830.t1 MTR_5g019480 32.090 134 87 2 36 169 36 165 6.64e-18 77.4
MS.gene031830.t1 MTR_7g087590 30.968 155 99 2 18 171 3 150 3.50e-17 77.0
MS.gene031830.t1 MTR_8g028920 32.800 125 79 4 35 157 6 127 1.07e-16 74.3
MS.gene031830.t1 MTR_4g073240 50.820 61 30 0 97 157 4 64 5.32e-16 69.7
MS.gene031830.t1 MTR_8g098595 36.667 90 55 2 70 157 23 112 1.69e-15 70.5
MS.gene031830.t1 MTR_8g076490 36.364 99 61 2 36 133 19 116 2.15e-15 70.1
MS.gene031830.t1 MTR_8g028920 36.905 84 51 2 76 157 5 88 8.51e-15 68.2
MS.gene031830.t1 MTR_5g021260 34.906 106 67 2 30 134 13 117 3.48e-14 67.0
MS.gene031830.t1 MTR_2g096690 44.615 65 36 0 57 121 30 94 8.92e-14 64.7
MS.gene031830.t1 MTR_4g121960 29.530 149 87 5 33 174 11 148 4.53e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031830.t1 AT3G20060 80.226 177 31 1 1 173 1 177 7.85e-101 288
MS.gene031830.t1 AT1G50490 81.111 180 31 1 1 177 1 180 2.79e-97 279
MS.gene031830.t1 AT3G20060 86.029 136 19 0 42 177 1 136 1.67e-86 250
MS.gene031830.t1 AT2G02760 46.154 143 76 1 30 171 2 144 1.24e-39 132
MS.gene031830.t1 AT2G02760 46.154 143 76 1 30 171 2 144 1.24e-39 132
MS.gene031830.t1 AT2G02760 46.154 143 76 1 30 171 2 144 1.24e-39 132
MS.gene031830.t1 AT2G02760 46.154 143 76 1 30 171 2 144 1.24e-39 132
MS.gene031830.t1 AT1G14400 46.154 143 76 1 30 171 2 144 1.29e-39 132
MS.gene031830.t1 AT1G14400 46.154 143 76 1 30 171 2 144 1.29e-39 132
MS.gene031830.t1 AT5G62540 46.479 142 74 2 30 169 2 143 4.15e-37 125
MS.gene031830.t1 AT1G78870 46.094 128 69 0 35 162 8 135 1.88e-35 121
MS.gene031830.t1 AT1G16890 45.312 128 70 0 35 162 8 135 7.44e-35 120
MS.gene031830.t1 AT5G56150 45.161 124 68 0 35 158 4 127 3.28e-34 118
MS.gene031830.t1 AT1G64230 44.355 124 69 0 35 158 4 127 1.37e-33 117
MS.gene031830.t1 AT1G64230 44.355 124 69 0 35 158 4 127 1.37e-33 117
MS.gene031830.t1 AT4G27960 41.860 129 75 0 35 163 4 132 1.62e-33 116
MS.gene031830.t1 AT2G16740 41.176 136 79 1 35 169 4 139 1.80e-33 116
MS.gene031830.t1 AT1G16890 46.154 130 68 1 35 162 15 144 2.68e-33 116
MS.gene031830.t1 AT3G08700 42.029 138 78 2 35 170 4 141 4.13e-33 115
MS.gene031830.t1 AT3G08700 42.029 138 78 2 35 170 4 141 4.13e-33 115
MS.gene031830.t1 AT3G08690 43.548 124 70 0 35 158 4 127 5.62e-33 115
MS.gene031830.t1 AT3G08690 43.548 124 70 0 35 158 4 127 5.62e-33 115
MS.gene031830.t1 AT5G53300 41.085 129 76 0 35 163 4 132 7.70e-33 115
MS.gene031830.t1 AT5G53300 41.085 129 76 0 35 163 4 132 7.70e-33 115
MS.gene031830.t1 AT5G53300 41.085 129 76 0 35 163 4 132 7.70e-33 115
MS.gene031830.t1 AT5G41700 42.742 124 71 0 35 158 4 127 1.07e-32 114
MS.gene031830.t1 AT5G41700 42.742 124 71 0 35 158 4 127 1.07e-32 114
MS.gene031830.t1 AT5G41700 42.742 124 71 0 35 158 4 127 1.07e-32 114
MS.gene031830.t1 AT4G27960 41.860 129 75 0 35 163 34 162 2.51e-32 114
MS.gene031830.t1 AT5G41700 43.200 125 70 1 35 158 4 128 6.09e-31 110
MS.gene031830.t1 AT1G64230 41.353 133 69 1 35 158 4 136 1.35e-30 109
MS.gene031830.t1 AT1G64230 46.602 103 55 0 56 158 67 169 1.10e-29 108
MS.gene031830.t1 AT1G64230 46.602 103 55 0 56 158 67 169 1.10e-29 108
MS.gene031830.t1 AT1G16890 50.526 95 47 0 68 162 8 102 4.60e-29 104
MS.gene031830.t1 AT1G78870 50.526 95 47 0 68 162 8 102 4.70e-29 104
MS.gene031830.t1 AT1G78870 45.714 105 57 0 35 139 8 112 5.20e-28 101
MS.gene031830.t1 AT1G78870 50.000 96 47 1 68 162 8 103 1.24e-27 100
MS.gene031830.t1 AT3G13550 37.500 136 85 0 35 170 39 174 4.63e-27 101
MS.gene031830.t1 AT1G36340 41.606 137 78 2 26 162 3 137 1.99e-26 98.6
MS.gene031830.t1 AT1G36340 41.606 137 78 2 26 162 3 137 1.99e-26 98.6
MS.gene031830.t1 AT5G56150 43.000 100 57 0 35 134 4 103 1.39e-25 95.1
MS.gene031830.t1 AT5G50870 41.129 124 68 3 34 155 4 124 4.89e-25 96.3
MS.gene031830.t1 AT5G50870 41.129 124 68 3 34 155 4 124 6.32e-25 96.3
MS.gene031830.t1 AT5G41700 40.385 104 62 0 35 138 4 107 2.24e-24 93.2
MS.gene031830.t1 AT3G13550 40.000 110 66 0 35 144 39 148 2.57e-24 93.6
MS.gene031830.t1 AT5G53300 40.404 99 59 0 35 133 4 102 7.98e-24 90.5
MS.gene031830.t1 AT3G55380 35.115 131 71 2 57 173 31 161 9.60e-24 92.0
MS.gene031830.t1 AT5G59300 35.115 131 71 2 57 173 30 160 9.99e-24 92.0
MS.gene031830.t1 AT3G46460 35.878 131 70 2 57 173 30 160 1.82e-23 91.3
MS.gene031830.t1 AT5G25760 39.252 107 64 1 52 157 25 131 1.14e-22 89.0
MS.gene031830.t1 AT5G25760 39.252 107 64 1 52 157 25 131 1.14e-22 89.0
MS.gene031830.t1 AT1G63800 36.641 131 66 4 35 157 6 127 1.32e-20 84.3
MS.gene031830.t1 AT3G57870 36.620 142 82 3 36 169 9 150 1.35e-20 84.0
MS.gene031830.t1 AT5G41340 36.641 131 66 4 35 157 6 127 5.49e-20 82.8
MS.gene031830.t1 AT2G18600 32.787 122 81 1 36 157 35 155 6.01e-19 80.1
MS.gene031830.t1 AT2G18600 32.787 122 81 1 36 157 35 155 6.01e-19 80.1
MS.gene031830.t1 AT1G63800 40.217 92 53 2 68 157 21 112 7.93e-19 79.3
MS.gene031830.t1 AT2G46030 34.400 125 77 3 35 157 6 127 1.77e-18 79.0
MS.gene031830.t1 AT2G46030 34.400 125 77 3 35 157 6 127 1.77e-18 79.0
MS.gene031830.t1 AT5G05080 33.099 142 93 2 32 172 10 150 6.33e-18 79.0
MS.gene031830.t1 AT5G05080 33.099 142 93 2 32 172 10 150 6.33e-18 79.0
MS.gene031830.t1 AT5G05080 33.099 142 93 2 32 172 10 150 6.33e-18 79.0
MS.gene031830.t1 AT5G05080 33.099 142 93 2 32 172 10 150 6.33e-18 79.0
MS.gene031830.t1 AT2G46030 34.400 125 77 3 35 157 78 199 1.63e-17 77.8
MS.gene031830.t1 AT4G36800 31.148 122 83 1 36 157 34 154 4.59e-17 75.1
MS.gene031830.t1 AT4G36800 31.148 122 83 1 36 157 34 154 4.59e-17 75.1
MS.gene031830.t1 AT3G55380 27.907 172 75 4 51 173 24 195 2.23e-16 73.6
MS.gene031830.t1 AT3G46460 33.333 111 60 2 77 173 8 118 5.28e-16 70.9
MS.gene031830.t1 AT3G46460 33.333 111 60 2 77 173 8 118 5.28e-16 70.9
MS.gene031830.t1 AT2G46030 35.644 101 63 2 59 157 30 130 7.23e-16 72.0
MS.gene031830.t1 AT2G46030 35.644 101 63 2 59 157 30 130 7.23e-16 72.0
MS.gene031830.t1 AT5G41340 40.000 85 49 2 75 157 8 92 7.58e-16 71.2
MS.gene031830.t1 AT3G24515 37.391 115 63 4 68 173 51 165 9.97e-16 74.3
MS.gene031830.t1 AT3G24515 37.391 115 63 4 68 173 76 190 1.44e-15 73.9
MS.gene031830.t1 AT1G45050 33.929 112 72 2 24 134 7 117 6.12e-15 68.9
MS.gene031830.t1 AT1G75440 34.234 111 71 2 24 133 7 116 2.32e-14 67.4
MS.gene031830.t1 AT5G42990 33.929 112 72 2 24 134 7 117 2.95e-14 67.4
MS.gene031830.t1 AT4G36410 35.354 99 62 2 36 133 19 116 4.26e-14 67.0
MS.gene031830.t1 AT2G32790 38.017 121 72 2 53 173 51 168 4.91e-14 67.0
MS.gene031830.t1 AT3G17000 32.743 113 67 3 34 141 13 121 8.79e-12 62.8

Find 36 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACTTTAGGAGCCTTAAA+AGG 0.184751 4.3:+32069367 None:intergenic
TTGTTTGTTTATAGGATAAA+TGG 0.261888 4.3:-32069191 MS.gene031830:intron
CATCCCAATGTTGATCTTCA+TGG 0.269716 4.3:-32069325 MS.gene031830:CDS
GCCTTCAAACACTGTATCTT+TGG 0.285480 4.3:+32069429 None:intergenic
TTGTGGAAAGGAACAATAAC+AGG 0.324345 4.3:-32069454 MS.gene031830:CDS
CTGTCCATCCAAAGTCTTCT+TGG 0.336173 4.3:-32069138 MS.gene031830:CDS
AGCATCAGTGTTCCCATTGA+TGG 0.378850 4.3:+32070557 None:intergenic
GTAGTTTCAAACTTGACTTT+AGG 0.379535 4.3:+32069355 None:intergenic
ATCCACAAGCAGCACAGCTT+TGG 0.411990 4.3:-32068902 MS.gene031830:CDS
GCCTTAAAAGGGTAATCATT+AGG 0.415557 4.3:+32069379 None:intergenic
TGTCAGATGAGTGGTGAATC+TGG 0.420691 4.3:-32069511 MS.gene031830:intron
TATATGCTTGGACATACTTC+AGG 0.420859 4.3:-32069299 MS.gene031830:intron
GTCTGAATTGATGTCTTTGA+TGG 0.426742 4.3:-32069744 MS.gene031830:intron
CTCCAAAGCTGTGCTGCTTG+TGG 0.432715 4.3:+32068900 None:intergenic
ATTCCACCTCTGTTCTCAAA+AGG 0.440278 4.3:-32070479 MS.gene031830:intron
TGTGCTGCTTGTGGATTTAG+TGG 0.440911 4.3:+32068909 None:intergenic
TTTAGTGGTGAACTAATGTT+TGG 0.452232 4.3:+32068924 None:intergenic
GTCTACCTCCAAGAAGACTT+TGG 0.472732 4.3:+32069130 None:intergenic
TGACTTTAGGAGCCTTAAAA+GGG 0.487045 4.3:+32069368 None:intergenic
ATCAACATTGGGATGGAAGC+AGG 0.492019 4.3:+32069333 None:intergenic
TTATACAGATTACAGAAAGA+TGG 0.493470 4.3:-32068781 MS.gene031830:intron
TTGCCATGAAGATCAACATT+GGG 0.507321 4.3:+32069322 None:intergenic
TCTTCATGGCAATATATGCT+TGG 0.513262 4.3:-32069311 MS.gene031830:CDS
GAGGTGGAATCAACGGACTT+AGG 0.525977 4.3:+32070492 None:intergenic
ATCGACTAAGCAGTAGCACT+TGG 0.531003 4.3:+32068738 None:intergenic
ACCTCCAAGAAGACTTTGGA+TGG 0.537043 4.3:+32069134 None:intergenic
TCCATCCAAAGTCTTCTTGG+AGG 0.548893 4.3:-32069135 MS.gene031830:intron
ACCAAAGATACAGTGTTTGA+AGG 0.558474 4.3:-32069430 MS.gene031830:CDS
ACGGACTTAGGAGGAGGAAG+AGG 0.570689 4.3:+32070504 None:intergenic
GAGAACAGAGGTGGAATCAA+CGG 0.579252 4.3:+32070485 None:intergenic
ATTGCCATGAAGATCAACAT+TGG 0.588583 4.3:+32069321 None:intergenic
GAATCAACGGACTTAGGAGG+AGG 0.597125 4.3:+32070498 None:intergenic
GTGGAATCAACGGACTTAGG+AGG 0.610762 4.3:+32070495 None:intergenic
CAGCTTTGGAGCAATCAAGA+AGG 0.619795 4.3:-32068888 MS.gene031830:intron
TACAGATTACAGAAAGATGG+TGG 0.634223 4.3:-32068778 MS.gene031830:intron
CATGAAGATCAACATTGGGA+TGG 0.643434 4.3:+32069326 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAGTTAAAAAAGAATT+AGG + chr4.3:32070352-32070371 None:intergenic 10.0%
!!! TTATGTTCTTTTTTTTTTTT+TGG - chr4.3:32068960-32068979 MS.gene031830:intron 10.0%
!! AAAAAAAAAAAGAACATAAG+TGG + chr4.3:32068959-32068978 None:intergenic 15.0%
!! AAAAAAAAAAGAACATAAGT+GGG + chr4.3:32068958-32068977 None:intergenic 15.0%
!! ACAAATGACAATAATAATAA+GGG + chr4.3:32069451-32069470 None:intergenic 15.0%
!! TAAAAGAGAGATTTAATAAT+AGG + chr4.3:32068865-32068884 None:intergenic 15.0%
!! TACAAATGACAATAATAATA+AGG + chr4.3:32069452-32069471 None:intergenic 15.0%
!!! TATATGTGTAAAACTATTTT+AGG - chr4.3:32070282-32070301 MS.gene031830:intron 15.0%
!! ACACTAATCTAAATTGTATA+CGG + chr4.3:32070314-32070333 None:intergenic 20.0%
!! TTGTTTGTTTATAGGATAAA+TGG - chr4.3:32070116-32070135 MS.gene031830:intron 20.0%
!! TTGTTTGTTTGTTTGTTTAT+AGG - chr4.3:32070108-32070127 MS.gene031830:intron 20.0%
! TTATACAGATTACAGAAAGA+TGG - chr4.3:32070526-32070545 MS.gene031830:CDS 25.0%
AAAAATATGTTGTCTTCCTC+AGG + chr4.3:32069835-32069854 None:intergenic 30.0%
ACAAGACTCATAATGATTGA+AGG + chr4.3:32070452-32070471 None:intergenic 30.0%
ACAATGTGTGAATGTAACTT+TGG + chr4.3:32069707-32069726 None:intergenic 30.0%
AGGTAATGTGAAATCATCTA+AGG - chr4.3:32070028-32070047 MS.gene031830:intron 30.0%
ATGTTAATGTATGATGTCGT+CGG + chr4.3:32069033-32069052 None:intergenic 30.0%
CATACATTAACATGATCAGT+GGG - chr4.3:32069040-32069059 MS.gene031830:intron 30.0%
GTAGTTTCAAACTTGACTTT+AGG + chr4.3:32069955-32069974 None:intergenic 30.0%
TCATACATTAACATGATCAG+TGG - chr4.3:32069039-32069058 MS.gene031830:intron 30.0%
TCTGAACCTCAACAAAATTA+AGG + chr4.3:32069677-32069696 None:intergenic 30.0%
TGAAAAAACTAAGCTACACA+AGG + chr4.3:32069376-32069395 None:intergenic 30.0%
TTTAGTGGTGAACTAATGTT+TGG + chr4.3:32070386-32070405 None:intergenic 30.0%
! AATCATTATGAGTCTTGTGA+TGG - chr4.3:32070454-32070473 MS.gene031830:intron 30.0%
! ATCACACTGATTGTGATAAA+TGG - chr4.3:32069206-32069225 MS.gene031830:intron 30.0%
! TCCTAATGATTACCCTTTTA+AGG - chr4.3:32069927-32069946 MS.gene031830:intron 30.0%
!! AGGAAGACAACATATTTTTG+TGG - chr4.3:32069836-32069855 MS.gene031830:intron 30.0%
!! GACAACATATTTTTGTGGAA+AGG - chr4.3:32069841-32069860 MS.gene031830:intron 30.0%
!!! ACACGTTTTTTGTTTTGTGT+TGG - chr4.3:32069508-32069527 MS.gene031830:CDS 30.0%
!!! ATTTGTTGTTGACCTTGTTT+TGG - chr4.3:32070066-32070085 MS.gene031830:intron 30.0%
!!! CACGTTCATTATTGTTTTTG+AGG - chr4.3:32069626-32069645 MS.gene031830:intron 30.0%
AAAATGACCGGAATGATGAA+CGG + chr4.3:32069159-32069178 None:intergenic 35.0%
ACCAAAGATACAGTGTTTGA+AGG - chr4.3:32069877-32069896 MS.gene031830:intron 35.0%
ACTACAAGTGTCTAAGTAAG+TGG - chr4.3:32069308-32069327 MS.gene031830:CDS 35.0%
ATGTAGCCGTAACACATAAA+TGG + chr4.3:32069338-32069357 None:intergenic 35.0%
ATTGCCATGAAGATCAACAT+TGG + chr4.3:32069989-32070008 None:intergenic 35.0%
CATGTATTTAGCCAATAGTG+TGG - chr4.3:32069735-32069754 MS.gene031830:intron 35.0%
CTACAAGTGTCTAAGTAAGT+GGG - chr4.3:32069309-32069328 MS.gene031830:CDS 35.0%
CTTGTATGCATTCTTAGAAG+AGG - chr4.3:32069073-32069092 MS.gene031830:intron 35.0%
GCCTTAAAAGGGTAATCATT+AGG + chr4.3:32069931-32069950 None:intergenic 35.0%
GCTCAACACACAAATCAAAT+TGG + chr4.3:32069535-32069554 None:intergenic 35.0%
GTCTGAATTGATGTCTTTGA+TGG - chr4.3:32069563-32069582 MS.gene031830:intron 35.0%
TACAGATTACAGAAAGATGG+TGG - chr4.3:32070529-32070548 MS.gene031830:CDS 35.0%
TATATGCTTGGACATACTTC+AGG - chr4.3:32070008-32070027 MS.gene031830:intron 35.0%
TCTTCATGGCAATATATGCT+TGG - chr4.3:32069996-32070015 MS.gene031830:intron 35.0%
TTGCCATGAAGATCAACATT+GGG + chr4.3:32069988-32070007 None:intergenic 35.0%
TTGTGGAAAGGAACAATAAC+AGG - chr4.3:32069853-32069872 MS.gene031830:intron 35.0%
TTTCAACTGATCCACACTAT+TGG + chr4.3:32069749-32069768 None:intergenic 35.0%
! TGACTTTAGGAGCCTTAAAA+GGG + chr4.3:32069942-32069961 None:intergenic 35.0%
! TGTTTTGTAGGAGCAACAAT+GGG + chr4.3:32068782-32068801 None:intergenic 35.0%
! TTGACTTTAGGAGCCTTAAA+AGG + chr4.3:32069943-32069962 None:intergenic 35.0%
!! ATGTCACTTTGGAAAATGAC+CGG + chr4.3:32069171-32069190 None:intergenic 35.0%
!! TAGGAGTGCTAGTTAAGAAA+TGG + chr4.3:32069413-32069432 None:intergenic 35.0%
!! TGTTCGCTTTTATGTCACTT+TGG + chr4.3:32069182-32069201 None:intergenic 35.0%
!! TTGTTTTGTAGGAGCAACAA+TGG + chr4.3:32068783-32068802 None:intergenic 35.0%
!!! TGCATTTTTTGTCAGATGAG+TGG - chr4.3:32069787-32069806 MS.gene031830:intron 35.0%
ACTTGTGTGATCCACATCAA+TGG - chr4.3:32069096-32069115 MS.gene031830:intron 40.0%
ATTCCACCTCTGTTCTCAAA+AGG - chr4.3:32068828-32068847 MS.gene031830:intron 40.0%
CATCCCAATGTTGATCTTCA+TGG - chr4.3:32069982-32070001 MS.gene031830:intron 40.0%
CATGAAGATCAACATTGGGA+TGG + chr4.3:32069984-32070003 None:intergenic 40.0%
CCTTATACCGTTCATCATTC+CGG - chr4.3:32069149-32069168 MS.gene031830:CDS 40.0%
GCCTTCAAACACTGTATCTT+TGG + chr4.3:32069881-32069900 None:intergenic 40.0%
GGGCATCCATTTATGTGTTA+CGG - chr4.3:32069329-32069348 MS.gene031830:CDS 40.0%
TGATGAACGGTATAAGGTGT+CGG + chr4.3:32069146-32069165 None:intergenic 40.0%
! GTGACGCCTTAATTTTGTTG+AGG - chr4.3:32069668-32069687 MS.gene031830:intron 40.0%
!! AAACCTTTTGAGAACAGAGG+TGG + chr4.3:32068834-32068853 None:intergenic 40.0%
!! AGGGTTTGCTAACTAGTGTT+AGG + chr4.3:32069432-32069451 None:intergenic 40.0%
!! GAGAAACCTTTTGAGAACAG+AGG + chr4.3:32068837-32068856 None:intergenic 40.0%
!! TGGTATATCTGCTTTTCCTG+AGG - chr4.3:32069816-32069835 MS.gene031830:intron 40.0%
!!! TTGTTGACCTTGTTTTGGCT+TGG - chr4.3:32070071-32070090 MS.gene031830:intron 40.0%
ACCTCCAAGAAGACTTTGGA+TGG + chr4.3:32070176-32070195 None:intergenic 45.0%
AGCATCAGTGTTCCCATTGA+TGG + chr4.3:32068753-32068772 None:intergenic 45.0%
ATCAACATTGGGATGGAAGC+AGG + chr4.3:32069977-32069996 None:intergenic 45.0%
CAGTATGCCAAGCCAAAACA+AGG + chr4.3:32070081-32070100 None:intergenic 45.0%
CATGATCAGTGGGTCTTTGT+TGG - chr4.3:32069050-32069069 MS.gene031830:intron 45.0%
CCGGAATGATGAACGGTATA+AGG + chr4.3:32069152-32069171 None:intergenic 45.0%
CTGTCCATCCAAAGTCTTCT+TGG - chr4.3:32070169-32070188 MS.gene031830:intron 45.0%
GAGAACAGAGGTGGAATCAA+CGG + chr4.3:32068825-32068844 None:intergenic 45.0%
GTCTACCTCCAAGAAGACTT+TGG + chr4.3:32070180-32070199 None:intergenic 45.0%
TCCATCCAAAGTCTTCTTGG+AGG - chr4.3:32070172-32070191 MS.gene031830:intron 45.0%
TGATCCACATCAATGGCACA+CGG - chr4.3:32069103-32069122 MS.gene031830:intron 45.0%
TGTCAGATGAGTGGTGAATC+TGG - chr4.3:32069796-32069815 MS.gene031830:intron 45.0%
!! CAGCTTTGGAGCAATCAAGA+AGG - chr4.3:32070419-32070438 MS.gene031830:intron 45.0%
!! GGAGGAAGAGGTTGTTTTGT+AGG + chr4.3:32068794-32068813 None:intergenic 45.0%
!! TGTGCTGCTTGTGGATTTAG+TGG + chr4.3:32070401-32070420 None:intergenic 45.0%
ATCCACAAGCAGCACAGCTT+TGG - chr4.3:32070405-32070424 MS.gene031830:intron 50.0%
! GAATCAACGGACTTAGGAGG+AGG + chr4.3:32068812-32068831 None:intergenic 50.0%
! GAGGTGGAATCAACGGACTT+AGG + chr4.3:32068818-32068837 None:intergenic 50.0%
! GTGGAATCAACGGACTTAGG+AGG + chr4.3:32068815-32068834 None:intergenic 50.0%
ACGGACTTAGGAGGAGGAAG+AGG + chr4.3:32068806-32068825 None:intergenic 55.0%
! GACACCGTGTGCCATTGATG+TGG + chr4.3:32069110-32069129 None:intergenic 55.0%
!! CTCCAAAGCTGTGCTGCTTG+TGG + chr4.3:32070410-32070429 None:intergenic 55.0%
!! AAGGTGTCGGTGCACTCTGC+AGG + chr4.3:32069133-32069152 None:intergenic 60.0%
!! AGGTGTCGGTGCACTCTGCA+GGG + chr4.3:32069132-32069151 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 32068743 32070586 32068743 ID=MS.gene031830
chr4.3 mRNA 32068743 32070586 32068743 ID=MS.gene031830.t1;Parent=MS.gene031830
chr4.3 exon 32070480 32070586 32070480 ID=MS.gene031830.t1.exon1;Parent=MS.gene031830.t1
chr4.3 CDS 32070480 32070586 32070480 ID=cds.MS.gene031830.t1;Parent=MS.gene031830.t1
chr4.3 exon 32069745 32069772 32069745 ID=MS.gene031830.t1.exon2;Parent=MS.gene031830.t1
chr4.3 CDS 32069745 32069772 32069745 ID=cds.MS.gene031830.t1;Parent=MS.gene031830.t1
chr4.3 exon 32069300 32069527 32069300 ID=MS.gene031830.t1.exon3;Parent=MS.gene031830.t1
chr4.3 CDS 32069300 32069527 32069300 ID=cds.MS.gene031830.t1;Parent=MS.gene031830.t1
chr4.3 exon 32069136 32069199 32069136 ID=MS.gene031830.t1.exon4;Parent=MS.gene031830.t1
chr4.3 CDS 32069136 32069199 32069136 ID=cds.MS.gene031830.t1;Parent=MS.gene031830.t1
chr4.3 exon 32068889 32068948 32068889 ID=MS.gene031830.t1.exon5;Parent=MS.gene031830.t1
chr4.3 CDS 32068889 32068948 32068889 ID=cds.MS.gene031830.t1;Parent=MS.gene031830.t1
chr4.3 exon 32068743 32068795 32068743 ID=MS.gene031830.t1.exon6;Parent=MS.gene031830.t1
chr4.3 CDS 32068743 32068795 32068743 ID=cds.MS.gene031830.t1;Parent=MS.gene031830.t1
Gene Sequence

>MS.gene031830

ATGGCTACCATCAATGGGAACACTGATGCTGCTCAACCCATTGTTGCTCCTACAAAACAACCTCTTCCTCCTCCTAAGTCCGTTGATTCCACCTCTGTTCTCAAAAGGTTTCTCATTCACCTATTATTAAATCTCTCTTTTACTTGTTTTTGTTTCATGTCAATATGTGTTAACTTGTGTTTTTTCAATCATATAATATAATTTCGCTTCATCCCACTTATGTTCTTTTTTTTTTTTTGGTACAAATGTTCTTTTTAAAATAATGTATGCTGACATTCAAAGTAATACCGACGACATCATACATTAACATGATCAGTGGGTCTTTGTTGGCTTGTATGCATTCTTAGAAGAGGACTTGTGTGATCCACATCAATGGCACACGGTGTCCCTGCAGAGTGCACCGACACCTTATACCGTTCATCATTCCGGTCATTTTCCAAAGTGACATAAAAGCGAACAAATGATCACACTGATTGTGATAAATGGTGTCAATATTCACTTTGAGTAATAGCACAACATTATTGTATATTTAATTAGTTTCATACTTTCATTATTTTCTTAAATTACTACAAGTGTCTAAGTAAGTGGGCATCCATTTATGTGTTACGGCTACATAGATCAACTGTGATGCCTTGTGTAGCTTAGTTTTTTCAAATCAATGAGTTAACCATTTCTTAACTAGCACTCCTAACACTAGTTAGCAAACCCTTATTATTATTGTCATTTGTAACAGCATTTGATTTGATTTGATTTGATTTGATTCTAACACGTTTTTTGTTTTGTGTTGGTCCAATTTGATTTGTGTGTTGAGCAGATTGCAGTCTGAATTGATGTCTTTGATGGTAAGCTTATATCAACTATCATTAATCATTATTGATTCTAACACGTTCATTATTGTTTTTGAGGCTGCTTGTTTAGAATCACAGTGACGCCTTAATTTTGTTGAGGTTCAGAGTTTTTGCCAAAGTTACATTCACACATTGTGAATCAAACATGTATTTAGCCAATAGTGTGGATCAGTTGAAAACATAGATATTAATGTTTTGCATTTTTTGTCAGATGAGTGGTGAATCTGGTATATCTGCTTTTCCTGAGGAAGACAACATATTTTTGTGGAAAGGAACAATAACAGGAACCAAAGATACAGTGTTTGAAGGCACAGATTACAAGTTATCACTTTCTTTTCCTAATGATTACCCTTTTAAGGCTCCTAAAGTCAAGTTTGAAACTACCTGCTTCCATCCCAATGTTGATCTTCATGGCAATATATGCTTGGACATACTTCAGGTAATGTGAAATCATCTAAGGAACATTTTAATGAGCATTTGTTGTTGACCTTGTTTTGGCTTGGCATACTGATCTTATTTGTTTGTTTGTTTGTTTATAGGATAAATGGTCATCTGCTTATGATGTCAGAACAATTCTACTGTCCATCCAAAGTCTTCTTGGAGGTAGACACAATGATTGAAAATTTTGTGCACACTTTAATTAATTGTTTTCTGAAATTTGCATGCTGTTAACAAGTTTTTCATATGCACATATATGTGTAAAACTATTTTAGGCATGCACCGTATACAATTTAGATTAGTGTATTCTTTGTACTTTTCCTAATTCTTTTTTAACTTTTTAATAATGTAGAGCCAAACATTAGTTCACCACTAAATCCACAAGCAGCACAGCTTTGGAGCAATCAAGAAGGTAATAGTCCTTCAATCATTATGAGTCTTGTGATGGATATAATTTGTGAATCTCAAATATAGTTATAATGATTTTTGATGATTTGTTATACAGATTACAGAAAGATGGTGGAGAAGTTGTACAAAGCTCCAAGTGCTACTGCTTAG
Protein sequence

>MS.gene031830.t1

MATINGNTDAAQPIVAPTKQPLPPPKSVDSTSVLKRLQSELMSLMMSGESGISAFPEEDNIFLWKGTITGTKDTVFEGTDYKLSLSFPNDYPFKAPKVKFETTCFHPNVDLHGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNPQAAQLWSNQEDYRKMVEKLYKAPSATA