AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024700.t1 MTR_5g009020 98.305 177 3 0 1 177 1 177 2.34e-131 365
MS.gene024700.t1 MTR_4g076200 85.876 177 25 0 1 177 1 177 6.78e-115 323
MS.gene024700.t1 MTR_4g076200 91.176 136 12 0 42 177 1 136 6.56e-94 269
MS.gene024700.t1 MTR_1g095510 64.286 168 59 1 6 172 15 182 5.55e-74 221
MS.gene024700.t1 MTR_1g095510 67.969 128 41 0 45 172 1 128 9.23e-62 187
MS.gene024700.t1 MTR_1g095510 57.738 168 50 2 6 172 15 162 1.27e-60 186
MS.gene024700.t1 MTR_4g108080 45.652 138 74 1 35 171 7 144 8.57e-39 130
MS.gene024700.t1 MTR_8g468530 44.928 138 75 1 35 171 23 160 2.71e-38 129
MS.gene024700.t1 MTR_2g078010 45.312 128 70 0 35 162 8 135 5.05e-35 120
MS.gene024700.t1 MTR_2g078010 45.312 128 70 0 35 162 8 135 6.87e-35 120
MS.gene024700.t1 MTR_8g071040 42.424 132 75 1 35 165 4 135 4.97e-33 115
MS.gene024700.t1 MTR_4g073240 43.089 123 70 0 35 157 4 126 3.77e-32 113
MS.gene024700.t1 MTR_5g075580 41.406 128 75 0 35 162 4 131 5.51e-32 112
MS.gene024700.t1 MTR_3g062450 41.406 128 75 0 35 162 4 131 5.51e-32 112
MS.gene024700.t1 MTR_7g116940 40.458 131 77 1 35 164 4 134 1.24e-31 111
MS.gene024700.t1 MTR_2g078010 40.845 142 70 1 35 162 8 149 1.31e-31 112
MS.gene024700.t1 MTR_1g021150 41.406 128 75 0 35 162 4 131 2.68e-31 110
MS.gene024700.t1 MTR_1g021150 39.231 130 79 0 33 162 10 139 6.76e-29 105
MS.gene024700.t1 MTR_4g108080 46.154 104 56 0 35 138 7 110 8.49e-29 103
MS.gene024700.t1 MTR_7g116940 38.406 138 77 2 35 164 4 141 1.42e-28 103
MS.gene024700.t1 MTR_4g073240 47.872 94 49 0 64 157 4 97 1.87e-28 102
MS.gene024700.t1 MTR_4g108080 51.000 100 48 1 73 171 45 144 2.63e-28 103
MS.gene024700.t1 MTR_5g075580 44.118 102 57 0 61 162 1 102 4.16e-28 101
MS.gene024700.t1 MTR_2g078010 45.545 101 55 0 35 135 8 108 3.67e-27 99.0
MS.gene024700.t1 MTR_4g063490 35.252 139 76 2 49 173 22 160 6.17e-26 97.8
MS.gene024700.t1 MTR_8g085690 37.097 124 78 0 35 158 25 148 2.81e-24 93.6
MS.gene024700.t1 MTR_7g116940 39.623 106 64 0 35 140 4 109 2.95e-24 92.4
MS.gene024700.t1 MTR_3g098780 38.211 123 74 2 36 156 6 128 9.94e-24 92.8
MS.gene024700.t1 MTR_3g098780 37.600 125 76 2 36 158 30 154 1.16e-23 93.2
MS.gene024700.t1 MTR_2g096690 35.115 131 71 2 57 173 30 160 6.66e-23 89.7
MS.gene024700.t1 MTR_2g096690 34.615 130 72 1 57 173 30 159 7.88e-23 89.7
MS.gene024700.t1 MTR_3g110110 36.607 112 70 1 47 157 20 131 2.93e-22 87.8
MS.gene024700.t1 MTR_5g016630 34.932 146 87 3 36 173 8 153 3.10e-21 85.5
MS.gene024700.t1 MTR_4g094872 35.915 142 83 3 36 169 8 149 4.70e-21 84.7
MS.gene024700.t1 MTR_4g106690 36.000 125 75 4 35 157 6 127 3.42e-20 83.2
MS.gene024700.t1 MTR_8g028920 34.400 125 77 4 35 157 6 127 5.21e-18 77.4
MS.gene024700.t1 MTR_5g019480 38.824 85 52 0 73 157 21 105 5.78e-18 76.3
MS.gene024700.t1 MTR_5g019480 31.746 126 85 1 36 161 33 157 8.56e-18 77.0
MS.gene024700.t1 MTR_5g019480 32.787 122 81 1 36 157 36 156 1.07e-17 76.6
MS.gene024700.t1 MTR_7g087590 30.496 141 97 1 32 171 10 150 3.25e-16 74.3
MS.gene024700.t1 MTR_8g098595 36.667 90 55 2 70 157 23 112 6.16e-16 71.6
MS.gene024700.t1 MTR_4g073240 50.820 61 30 0 97 157 4 64 8.19e-16 69.3
MS.gene024700.t1 MTR_8g076490 36.364 99 61 2 36 133 19 116 1.94e-15 70.1
MS.gene024700.t1 MTR_8g028920 36.905 84 51 2 76 157 5 88 3.20e-15 69.3
MS.gene024700.t1 MTR_5g021260 36.364 99 61 2 36 133 19 116 5.31e-14 66.6
MS.gene024700.t1 MTR_2g096690 43.077 65 37 0 57 121 30 94 7.75e-13 62.4
MS.gene024700.t1 MTR_8g023380 32.759 116 69 3 31 141 4 115 5.33e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024700.t1 AT3G20060 85.256 156 23 0 18 173 22 177 1.40e-99 285
MS.gene024700.t1 AT1G50490 85.000 160 24 0 18 177 21 180 9.07e-94 270
MS.gene024700.t1 AT3G20060 87.879 132 16 0 42 173 1 132 2.77e-87 252
MS.gene024700.t1 AT1G14400 44.928 138 75 1 35 171 7 144 4.73e-38 128
MS.gene024700.t1 AT1G14400 44.928 138 75 1 35 171 7 144 4.73e-38 128
MS.gene024700.t1 AT2G02760 44.928 138 75 1 35 171 7 144 5.34e-38 128
MS.gene024700.t1 AT2G02760 44.928 138 75 1 35 171 7 144 5.34e-38 128
MS.gene024700.t1 AT2G02760 44.928 138 75 1 35 171 7 144 5.34e-38 128
MS.gene024700.t1 AT2G02760 44.928 138 75 1 35 171 7 144 5.34e-38 128
MS.gene024700.t1 AT5G62540 45.775 142 75 2 30 169 2 143 5.99e-37 125
MS.gene024700.t1 AT1G78870 46.094 128 69 0 35 162 8 135 1.23e-35 122
MS.gene024700.t1 AT1G16890 45.312 128 70 0 35 162 8 135 5.37e-35 120
MS.gene024700.t1 AT1G16890 46.923 130 67 1 35 162 15 144 3.93e-34 118
MS.gene024700.t1 AT5G56150 42.188 128 74 0 35 162 4 131 2.09e-32 113
MS.gene024700.t1 AT2G16740 39.706 136 81 1 35 169 4 139 3.55e-32 113
MS.gene024700.t1 AT1G64230 41.406 128 75 0 35 162 4 131 7.17e-32 112
MS.gene024700.t1 AT1G64230 41.406 128 75 0 35 162 4 131 7.17e-32 112
MS.gene024700.t1 AT4G27960 40.625 128 76 0 35 162 4 131 1.15e-31 112
MS.gene024700.t1 AT3G08700 40.580 138 80 2 35 170 4 141 1.49e-31 111
MS.gene024700.t1 AT3G08700 40.580 138 80 2 35 170 4 141 1.49e-31 111
MS.gene024700.t1 AT3G08690 41.935 124 72 0 35 158 4 127 3.64e-31 110
MS.gene024700.t1 AT3G08690 41.935 124 72 0 35 158 4 127 3.64e-31 110
MS.gene024700.t1 AT5G41700 39.844 128 77 0 35 162 4 131 5.38e-31 110
MS.gene024700.t1 AT5G41700 39.844 128 77 0 35 162 4 131 5.38e-31 110
MS.gene024700.t1 AT5G41700 39.844 128 77 0 35 162 4 131 5.38e-31 110
MS.gene024700.t1 AT5G53300 39.844 128 77 0 35 162 4 131 5.86e-31 110
MS.gene024700.t1 AT5G53300 39.844 128 77 0 35 162 4 131 5.86e-31 110
MS.gene024700.t1 AT5G53300 39.844 128 77 0 35 162 4 131 5.86e-31 110
MS.gene024700.t1 AT4G27960 40.625 128 76 0 35 162 34 161 1.58e-30 109
MS.gene024700.t1 AT1G16890 51.579 95 46 0 68 162 8 102 1.19e-29 105
MS.gene024700.t1 AT1G78870 51.579 95 46 0 68 162 8 102 1.43e-29 105
MS.gene024700.t1 AT5G41700 39.535 129 77 1 35 162 4 132 6.45e-29 104
MS.gene024700.t1 AT1G64230 38.686 137 75 1 35 162 4 140 1.20e-28 104
MS.gene024700.t1 AT1G78870 45.714 105 57 0 35 139 8 112 3.20e-28 102
MS.gene024700.t1 AT1G64230 42.727 110 62 1 56 164 67 176 4.08e-28 103
MS.gene024700.t1 AT1G64230 42.727 110 62 1 56 164 67 176 4.08e-28 103
MS.gene024700.t1 AT1G78870 51.042 96 46 1 68 162 8 103 6.17e-28 101
MS.gene024700.t1 AT3G13550 37.500 136 85 0 35 170 39 174 3.45e-26 99.0
MS.gene024700.t1 AT1G36340 40.876 137 79 2 26 162 3 137 1.78e-25 96.3
MS.gene024700.t1 AT1G36340 40.876 137 79 2 26 162 3 137 1.78e-25 96.3
MS.gene024700.t1 AT5G50870 40.000 135 73 4 34 166 4 132 1.83e-25 97.1
MS.gene024700.t1 AT5G50870 41.129 124 68 3 34 155 4 124 2.61e-25 97.1
MS.gene024700.t1 AT5G56150 41.000 100 59 0 35 134 4 103 7.21e-24 90.9
MS.gene024700.t1 AT3G55380 33.813 139 78 2 49 173 23 161 4.01e-23 90.5
MS.gene024700.t1 AT3G13550 39.091 110 67 0 35 144 39 148 6.62e-23 90.1
MS.gene024700.t1 AT5G59300 34.351 131 72 2 57 173 30 160 1.00e-22 89.4
MS.gene024700.t1 AT5G41700 38.462 104 64 0 35 138 4 107 1.32e-22 88.6
MS.gene024700.t1 AT3G46460 33.813 139 78 2 49 173 22 160 1.70e-22 89.0
MS.gene024700.t1 AT5G25760 36.290 124 73 2 35 157 13 131 2.50e-22 88.2
MS.gene024700.t1 AT5G25760 36.290 124 73 2 35 157 13 131 2.50e-22 88.2
MS.gene024700.t1 AT5G53300 38.384 99 61 0 35 133 4 102 5.22e-22 85.9
MS.gene024700.t1 AT1G63800 38.400 125 72 4 35 157 6 127 9.25e-22 87.4
MS.gene024700.t1 AT5G41340 37.600 125 73 4 35 157 6 127 6.42e-21 85.1
MS.gene024700.t1 AT3G57870 35.915 142 83 3 36 169 9 150 3.88e-20 82.4
MS.gene024700.t1 AT2G18600 33.333 153 92 5 7 157 11 155 1.70e-19 81.6
MS.gene024700.t1 AT2G18600 33.333 153 92 5 7 157 11 155 1.70e-19 81.6
MS.gene024700.t1 AT1G63800 40.217 92 53 2 68 157 21 112 2.06e-19 80.9
MS.gene024700.t1 AT2G46030 36.000 125 75 4 35 157 6 127 6.06e-19 80.1
MS.gene024700.t1 AT2G46030 36.000 125 75 4 35 157 6 127 6.06e-19 80.1
MS.gene024700.t1 AT2G46030 36.000 125 75 4 35 157 78 199 4.29e-18 79.3
MS.gene024700.t1 AT4G36800 31.061 132 89 2 27 157 24 154 2.24e-17 75.9
MS.gene024700.t1 AT4G36800 31.061 132 89 2 27 157 24 154 2.24e-17 75.9
MS.gene024700.t1 AT5G05080 31.410 156 98 3 18 172 3 150 7.83e-17 75.9
MS.gene024700.t1 AT5G05080 31.410 156 98 3 18 172 3 150 7.83e-17 75.9
MS.gene024700.t1 AT5G05080 31.410 156 98 3 18 172 3 150 7.83e-17 75.9
MS.gene024700.t1 AT5G05080 31.410 156 98 3 18 172 3 150 7.83e-17 75.9
MS.gene024700.t1 AT5G41340 40.000 85 49 2 75 157 8 92 2.81e-16 72.4
MS.gene024700.t1 AT3G24515 32.484 157 86 5 36 173 10 165 3.86e-16 75.5
MS.gene024700.t1 AT2G46030 35.644 101 63 2 59 157 30 130 4.66e-16 72.4
MS.gene024700.t1 AT2G46030 35.644 101 63 2 59 157 30 130 4.66e-16 72.4
MS.gene024700.t1 AT3G46460 33.333 111 60 2 77 173 8 118 6.62e-16 70.5
MS.gene024700.t1 AT3G46460 33.333 111 60 2 77 173 8 118 6.62e-16 70.5
MS.gene024700.t1 AT3G55380 26.590 173 79 3 49 173 23 195 8.23e-16 72.0
MS.gene024700.t1 AT3G24515 35.652 115 65 3 68 173 76 190 9.15e-16 74.3
MS.gene024700.t1 AT1G75440 33.333 111 72 2 24 133 7 116 2.88e-14 67.4
MS.gene024700.t1 AT1G45050 32.432 111 73 2 24 133 7 116 4.06e-14 67.0
MS.gene024700.t1 AT4G36410 34.286 105 67 2 30 133 13 116 9.59e-14 65.9
MS.gene024700.t1 AT5G42990 32.432 111 73 2 24 133 7 116 1.78e-13 65.1
MS.gene024700.t1 AT2G32790 35.246 122 76 1 53 174 51 169 1.29e-12 63.2

Find 39 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACCTTTGGAGCCTTAAA+AGG 0.231384 5.4:+3770558 None:intergenic
GATTGAGTGTCAACGGATTT+AGG 0.260118 5.4:+3771388 None:intergenic
CACAATATATTGTCCTCTTC+AGG 0.336865 5.4:+3770666 None:intergenic
CAGGTTGCAGTCTGAATTGA+TGG 0.339528 5.4:-3770999 MS.gene024700:CDS
TATTTGCTTGGACATTCTTC+AGG 0.340447 5.4:-3770490 MS.gene024700:intron
TGGCAGATGAGTGGAGATTC+TGG 0.360212 5.4:-3770702 MS.gene024700:intron
GTGGTGTCAAATTTGACCTT+TGG 0.405768 5.4:+3770546 None:intergenic
TTGCCATGCAAATCAACATT+GGG 0.409629 5.4:+3770513 None:intergenic
ATCCACAAGCAGCACAGCTT+TGG 0.411990 5.4:-3770175 MS.gene024700:CDS
GTCTGAATTGATGGCTTTGA+TGG 0.419788 5.4:-3770990 MS.gene024700:intron
TTTAGCGGCGAGCTAATGTT+TGG 0.426525 5.4:+3770197 None:intergenic
CACCCCAATGTTGATTTGCA+TGG 0.432591 5.4:-3770516 MS.gene024700:CDS
CTCCAAAGCTGTGCTGCTTG+TGG 0.432715 5.4:+3770173 None:intergenic
GGAGGGAGAGGTTGCATTGA+AGG 0.440179 5.4:+3771412 None:intergenic
ACACTCAATCTGTTCTCAAA+AGG 0.451346 5.4:-3771375 MS.gene024700:intron
AGCCTTAAAAGGATACTCAT+TGG 0.457477 5.4:+3770569 None:intergenic
GCCTTAAAAGGATACTCATT+GGG 0.466815 5.4:+3770570 None:intergenic
TTCTACAGAATACAGGAAGA+TGG 0.481814 5.4:-3770064 MS.gene024700:intron
TTTCAAATTTACCTCCTAGC+AGG 0.485419 5.4:+3770299 None:intergenic
AGCAAAGACACTGTCTTTGA+AGG 0.498680 5.4:-3770621 MS.gene024700:CDS
TGCTGGAAAGGAACAATAGC+AGG 0.507664 5.4:-3770645 MS.gene024700:CDS
GTGTCAACGGATTTAGGCGG+AGG 0.521333 5.4:+3771394 None:intergenic
GACAATATATTGTGCTGGAA+AGG 0.525589 5.4:-3770657 MS.gene024700:CDS
TTTGCATGGCAATATTTGCT+TGG 0.528158 5.4:-3770502 MS.gene024700:CDS
AAGAGGACAATATATTGTGC+TGG 0.531992 5.4:-3770662 MS.gene024700:CDS
ACGGATTTAGGCGGAGGGAG+AGG 0.544685 5.4:+3771400 None:intergenic
GAGAACAGATTGAGTGTCAA+CGG 0.546293 5.4:+3771381 None:intergenic
TTGTCAATTCAAAGCCTGCT+AGG 0.548402 5.4:-3770313 MS.gene024700:CDS
GAGATCAAAGTTTAAGCAGC+AGG 0.549093 5.4:+3770021 None:intergenic
ATTGCCATGCAAATCAACAT+TGG 0.581157 5.4:+3770512 None:intergenic
TGTGCTGCTTGTGGATTTAG+CGG 0.595779 5.4:+3770182 None:intergenic
GTGATTATTTGGCAGATGAG+TGG 0.606809 5.4:-3770711 MS.gene024700:intron
CAGCTTTGGAGCAATCAAGA+AGG 0.619795 5.4:-3770161 MS.gene024700:intron
TCAATTCAAAGCCTGCTAGG+AGG 0.657162 5.4:-3770310 MS.gene024700:intron
TGTCAACGGATTTAGGCGGA+GGG 0.657757 5.4:+3771395 None:intergenic
CATGCAAATCAACATTGGGG+TGG 0.679728 5.4:+3770517 None:intergenic
TGAGTGTCAACGGATTTAGG+CGG 0.688984 5.4:+3771391 None:intergenic
TGCCATGCAAATCAACATTG+GGG 0.689638 5.4:+3770514 None:intergenic
AACATTGGGGTGGAAGCATG+TGG 0.689650 5.4:+3770527 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATTTTTATTTTATTTTTG+TGG - chr5.4:3770455-3770474 MS.gene024700:intron 10.0%
!!! CTTTTTAATTTACTTAATTT+TGG - chr5.4:3770343-3770362 MS.gene024700:CDS 10.0%
!!! GTTTAGTTAATTTAAAATAT+TGG - chr5.4:3770395-3770414 MS.gene024700:intron 10.0%
!!! TTTTAATTTACTTAATTTTG+GGG - chr5.4:3770273-3770292 MS.gene024700:intron 10.0%
!! AAAAATACAATTAACAAACA+GGG + chr5.4:3771255-3771274 None:intergenic 15.0%
!! AATTCATTAATGTGATTATT+TGG - chr5.4:3770663-3770682 MS.gene024700:CDS 15.0%
!! ATTCATTAATGTGATTATTT+GGG - chr5.4:3770664-3770683 MS.gene024700:CDS 15.0%
!! TAAAAATACAATTAACAAAC+AGG + chr5.4:3771256-3771275 None:intergenic 15.0%
!!! AAGTGTTATATTATTGATTT+AGG + chr5.4:3771219-3771238 None:intergenic 15.0%
!!! AATTGATTAATCTGTTTTAT+AGG - chr5.4:3771118-3771137 MS.gene024700:intron 15.0%
!!! AGTTAATTTAAAATATTGGT+AGG - chr5.4:3770399-3770418 MS.gene024700:intron 15.0%
!!! GTTAATTTAAAATATTGGTA+GGG - chr5.4:3770400-3770419 MS.gene024700:intron 15.0%
!!! TGTTGATTAAATTAAAGTTA+GGG - chr5.4:3770309-3770328 MS.gene024700:intron 15.0%
!!! TGTTGATTGAATTAAAATTT+AGG - chr5.4:3770243-3770262 MS.gene024700:intron 15.0%
!!! TTGTTGATTAAATTAAAGTT+AGG - chr5.4:3770308-3770327 MS.gene024700:intron 15.0%
!! AAAAAGTAATTAGATGGTAA+TGG - chr5.4:3770620-3770639 MS.gene024700:CDS 20.0%
!! AAAAGTAATTAGATGGTAAT+GGG - chr5.4:3770621-3770640 MS.gene024700:CDS 20.0%
!! AAAATTGAGTTAGTTAGATT+AGG + chr5.4:3770442-3770461 None:intergenic 20.0%
!! GACTTAAAAAAGTAATTAGA+TGG - chr5.4:3770614-3770633 MS.gene024700:CDS 20.0%
!!! AATCTGTTTTATAGGATAAA+TGG - chr5.4:3771126-3771145 MS.gene024700:intron 20.0%
!!! ACTTAATTTTGGGAATTAAT+TGG - chr5.4:3770354-3770373 MS.gene024700:CDS 20.0%
! TTAAAAGAAATCAAACCCTT+CGG - chr5.4:3770194-3770213 MS.gene024700:CDS 25.0%
!! TAGTTTGTATGAAATCAGTT+GGG - chr5.4:3770736-3770755 MS.gene024700:intron 25.0%
!!! TTAGTTTGTATGAAATCAGT+TGG - chr5.4:3770735-3770754 MS.gene024700:intron 25.0%
AGTTTGTATGAAATCAGTTG+GGG - chr5.4:3770737-3770756 MS.gene024700:intron 30.0%
! ACTTTTCTTCTACAGAATAC+AGG - chr5.4:3771421-3771440 MS.gene024700:CDS 30.0%
! GTCTAAAGCAATGTAATTTG+AGG - chr5.4:3770592-3770611 MS.gene024700:CDS 30.0%
!! ACTTAATTTTGGGGATCTAT+TGG - chr5.4:3770282-3770301 MS.gene024700:intron 30.0%
!! GATAGATTTTGGGGATTAAT+GGG - chr5.4:3770216-3770235 MS.gene024700:intron 30.0%
!! TAGATGGTAATGGGATTATT+TGG - chr5.4:3770630-3770649 MS.gene024700:CDS 30.0%
AAGAGGACAATATATTGTGC+TGG - chr5.4:3770830-3770849 MS.gene024700:intron 35.0%
ACACTCAATCTGTTCTCAAA+AGG - chr5.4:3770117-3770136 MS.gene024700:intron 35.0%
AGCCTTAAAAGGATACTCAT+TGG + chr5.4:3770926-3770945 None:intergenic 35.0%
ATTGCCATGCAAATCAACAT+TGG + chr5.4:3770983-3771002 None:intergenic 35.0%
CACAATATATTGTCCTCTTC+AGG + chr5.4:3770829-3770848 None:intergenic 35.0%
GACAATATATTGTGCTGGAA+AGG - chr5.4:3770835-3770854 MS.gene024700:intron 35.0%
GCCTTAAAAGGATACTCATT+GGG + chr5.4:3770925-3770944 None:intergenic 35.0%
TATTTGCTTGGACATTCTTC+AGG - chr5.4:3771002-3771021 MS.gene024700:intron 35.0%
TTCTACAGAATACAGGAAGA+TGG - chr5.4:3771428-3771447 MS.gene024700:CDS 35.0%
TTGCCATGCAAATCAACATT+GGG + chr5.4:3770982-3771001 None:intergenic 35.0%
TTTCAAATTTACCTCCTAGC+AGG + chr5.4:3771196-3771215 None:intergenic 35.0%
TTTGCATGGCAATATTTGCT+TGG - chr5.4:3770990-3771009 MS.gene024700:intron 35.0%
! AAACCCTTCGGATAGATTTT+GGG - chr5.4:3770206-3770225 MS.gene024700:intron 35.0%
! TCCCAATGAGTATCCTTTTA+AGG - chr5.4:3770921-3770940 MS.gene024700:intron 35.0%
!! GGATAGATTTTGGGGATTAA+TGG - chr5.4:3770215-3770234 MS.gene024700:intron 35.0%
!! TGGCTTTGATGGTAAGAAAT+TGG - chr5.4:3770513-3770532 MS.gene024700:CDS 35.0%
AATCCCCAAAATCTATCCGA+AGG + chr5.4:3770213-3770232 None:intergenic 40.0%
AGCAAAGACACTGTCTTTGA+AGG - chr5.4:3770871-3770890 MS.gene024700:intron 40.0%
ATCCCCAAAATCTATCCGAA+GGG + chr5.4:3770212-3770231 None:intergenic 40.0%
GAGAACAGATTGAGTGTCAA+CGG + chr5.4:3770114-3770133 None:intergenic 40.0%
GTGATTATTTGGCAGATGAG+TGG - chr5.4:3770781-3770800 MS.gene024700:intron 40.0%
TGCCATGCAAATCAACATTG+GGG + chr5.4:3770981-3771000 None:intergenic 40.0%
TTGTCAATTCAAAGCCTGCT+AGG - chr5.4:3771179-3771198 MS.gene024700:intron 40.0%
! AACCCTTCGGATAGATTTTG+GGG - chr5.4:3770207-3770226 MS.gene024700:intron 40.0%
! CAAACCCTTCGGATAGATTT+TGG - chr5.4:3770205-3770224 MS.gene024700:intron 40.0%
! GATTGAGTGTCAACGGATTT+AGG + chr5.4:3770107-3770126 None:intergenic 40.0%
! GTGGTGTCAAATTTGACCTT+TGG + chr5.4:3770949-3770968 None:intergenic 40.0%
!! GTCTGAATTGATGGCTTTGA+TGG - chr5.4:3770502-3770521 MS.gene024700:CDS 40.0%
!! TGTATCTGCTTTTCCTGAAG+AGG - chr5.4:3770813-3770832 MS.gene024700:intron 40.0%
!! TTGACCTTTGGAGCCTTAAA+AGG + chr5.4:3770937-3770956 None:intergenic 40.0%
!!! GTATCCTTTTAAGGCTCCAA+AGG - chr5.4:3770930-3770949 MS.gene024700:intron 40.0%
CAGGTTGCAGTCTGAATTGA+TGG - chr5.4:3770493-3770512 MS.gene024700:CDS 45.0%
CATGCAAATCAACATTGGGG+TGG + chr5.4:3770978-3770997 None:intergenic 45.0%
GTGTCAGTAGCATGTGATGA+TGG - chr5.4:3771036-3771055 MS.gene024700:intron 45.0%
TCAATTCAAAGCCTGCTAGG+AGG - chr5.4:3771182-3771201 MS.gene024700:intron 45.0%
TGCTGGAAAGGAACAATAGC+AGG - chr5.4:3770847-3770866 MS.gene024700:intron 45.0%
TTTAGCGGCGAGCTAATGTT+TGG + chr5.4:3771298-3771317 None:intergenic 45.0%
! TGAGTGTCAACGGATTTAGG+CGG + chr5.4:3770104-3770123 None:intergenic 45.0%
!! CACCCCAATGTTGATTTGCA+TGG - chr5.4:3770976-3770995 MS.gene024700:intron 45.0%
!! CAGCTTTGGAGCAATCAAGA+AGG - chr5.4:3771331-3771350 MS.gene024700:intron 45.0%
!! GTGGGTTGTTGCAATTTTGC+AGG - chr5.4:3770474-3770493 MS.gene024700:intron 45.0%
!! TGTGCTGCTTGTGGATTTAG+CGG + chr5.4:3771313-3771332 None:intergenic 45.0%
!!! AATTTTTATTTTATTTTTGT+GGG - chr5.4:3770456-3770475 MS.gene024700:intron 5.0%
!!! TTTTTAATTTACTTAATTTT+GGG - chr5.4:3770272-3770291 MS.gene024700:intron 5.0%
!!! TTTTTTAATTTACTTAATTT+TGG - chr5.4:3770271-3770290 MS.gene024700:intron 5.0%
ATCCACAAGCAGCACAGCTT+TGG - chr5.4:3771317-3771336 MS.gene024700:intron 50.0%
TGGCAGATGAGTGGAGATTC+TGG - chr5.4:3770790-3770809 MS.gene024700:intron 50.0%
! AACATTGGGGTGGAAGCATG+TGG + chr5.4:3770968-3770987 None:intergenic 50.0%
! TGTCAACGGATTTAGGCGGA+GGG + chr5.4:3770100-3770119 None:intergenic 50.0%
GGAGGGAGAGGTTGCATTGA+AGG + chr5.4:3770083-3770102 None:intergenic 55.0%
! GTGTCAACGGATTTAGGCGG+AGG + chr5.4:3770101-3770120 None:intergenic 55.0%
!! CTCCAAAGCTGTGCTGCTTG+TGG + chr5.4:3771322-3771341 None:intergenic 55.0%
ACGGATTTAGGCGGAGGGAG+AGG + chr5.4:3770095-3770114 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 3770032 3771482 3770032 ID=MS.gene024700
chr5.4 mRNA 3770032 3771482 3770032 ID=MS.gene024700.t1;Parent=MS.gene024700
chr5.4 exon 3771376 3771482 3771376 ID=MS.gene024700.t1.exon1;Parent=MS.gene024700.t1
chr5.4 CDS 3771376 3771482 3771376 ID=cds.MS.gene024700.t1;Parent=MS.gene024700.t1
chr5.4 exon 3770991 3771018 3770991 ID=MS.gene024700.t1.exon2;Parent=MS.gene024700.t1
chr5.4 CDS 3770991 3771018 3770991 ID=cds.MS.gene024700.t1;Parent=MS.gene024700.t1
chr5.4 exon 3770491 3770718 3770491 ID=MS.gene024700.t1.exon3;Parent=MS.gene024700.t1
chr5.4 CDS 3770491 3770718 3770491 ID=cds.MS.gene024700.t1;Parent=MS.gene024700.t1
chr5.4 exon 3770311 3770374 3770311 ID=MS.gene024700.t1.exon4;Parent=MS.gene024700.t1
chr5.4 CDS 3770311 3770374 3770311 ID=cds.MS.gene024700.t1;Parent=MS.gene024700.t1
chr5.4 exon 3770162 3770221 3770162 ID=MS.gene024700.t1.exon5;Parent=MS.gene024700.t1
chr5.4 CDS 3770162 3770221 3770162 ID=cds.MS.gene024700.t1;Parent=MS.gene024700.t1
chr5.4 exon 3770032 3770078 3770032 ID=MS.gene024700.t1.exon6;Parent=MS.gene024700.t1
chr5.4 CDS 3770032 3770078 3770032 ID=cds.MS.gene024700.t1;Parent=MS.gene024700.t1
Gene Sequence

>MS.gene024700

ATGGCTAGCGCTAACGTTCAAACTGAAGCTTCTCAAAGCAACATTGCTCCTTCAATGCAACCTCTCCCTCCGCCTAAATCCGTTGACACTCAATCTGTTCTCAAAAGGTTCGTTTTTTCAATTCAGCATTACAGTTTTTTGAATCTTGTTTTGTTTTTCGATTTAAAAGAAATCAAACCCTTCGGATAGATTTTGGGGATTAATGGGTTTTTGTTGATTGAATTAAAATTTAGGTTTTTTTTTTTAATTTACTTAATTTTGGGGATCTATTGGTTTTTGTTGATTAAATTAAAGTTAGGGTTTTTACTTAACTTTTTAATTTACTTAATTTTGGGAATTAATTGGTTTTTGTTTATTTTTTTAGTTTAGTTAATTTAAAATATTGGTAGGGTTTTCAGTTTGTTTCTCCTAATCTAACTAACTCAATTTTTATTTTATTTTTGTGGGTTGTTGCAATTTTGCAGGTTGCAGTCTGAATTGATGGCTTTGATGGTAAGAAATTGGATCTCTCTTTTTCACTTTCTGTGATCTAATTTTGTGTTAGTTTATCAATTAGTTTTGTCTAAAGCAATGTAATTTGAGGACTTAAAAAAGTAATTAGATGGTAATGGGATTATTTGGAAAAGTGATTAATTCATTAATGTGATTATTTGGGCTCATTTATTTTTTATTCTTGTGTGTGTATGCAATGTAATTTGAGCACTTAGTTTGTATGAAATCAGTTGGGGAAAAGTGATTAATTCATTAATGTGATTATTTGGCAGATGAGTGGAGATTCTGGTGTATCTGCTTTTCCTGAAGAGGACAATATATTGTGCTGGAAAGGAACAATAGCAGGAAGCAAAGACACTGTCTTTGAAGGAACAGAATACAAATTGTCACTTTCATTTCCCAATGAGTATCCTTTTAAGGCTCCAAAGGTCAAATTTGACACCACATGCTTCCACCCCAATGTTGATTTGCATGGCAATATTTGCTTGGACATTCTTCAGGTAATGAATGTTGTGTCAGTAGCATGTGATGATGGTGACATCTTGTTATGTTGTGTCATTTTCAATACAACTATTGCATATTGAGATTATGATTAATTGATTAATCTGTTTTATAGGATAAATGGTCATCTGCTTATGATGTTAGAACAATTCTTTTGTCAATTCAAAGCCTGCTAGGAGGTAAATTTGAAACCTAAATCAATAATATAACACTTTTATTTTGTTCTTCCCTGTTTGTTAATTGTATTTTTACTTCTTTCATAATTTAGAGCCAAACATTAGCTCGCCGCTAAATCCACAAGCAGCACAGCTTTGGAGCAATCAAGAAGGTAAATAAACATAATTGATATTTTGTAGAGTATATCTTCTGAAAGATGAACTGATTTTGCTGTGTTCTACTTTTCTTCTACAGAATACAGGAAGATGGTTGAGAAGTTGTACAAAGATCCTGCTGCTTAA
Protein sequence

>MS.gene024700.t1

MASANVQTEASQSNIAPSMQPLPPPKSVDTQSVLKRLQSELMALMMSGDSGVSAFPEEDNILCWKGTIAGSKDTVFEGTEYKLSLSFPNEYPFKAPKVKFDTTCFHPNVDLHGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNPQAAQLWSNQEEYRKMVEKLYKDPAA