AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21952

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21952.t1 MTR_5g009020 98.305 177 3 0 1 177 1 177 2.34e-131 365
MS.gene21952.t1 MTR_4g076200 85.876 177 25 0 1 177 1 177 6.78e-115 323
MS.gene21952.t1 MTR_4g076200 91.176 136 12 0 42 177 1 136 6.56e-94 269
MS.gene21952.t1 MTR_1g095510 64.286 168 59 1 6 172 15 182 5.55e-74 221
MS.gene21952.t1 MTR_1g095510 67.969 128 41 0 45 172 1 128 9.23e-62 187
MS.gene21952.t1 MTR_1g095510 57.738 168 50 2 6 172 15 162 1.27e-60 186
MS.gene21952.t1 MTR_4g108080 45.652 138 74 1 35 171 7 144 8.57e-39 130
MS.gene21952.t1 MTR_8g468530 44.928 138 75 1 35 171 23 160 2.71e-38 129
MS.gene21952.t1 MTR_2g078010 45.312 128 70 0 35 162 8 135 5.05e-35 120
MS.gene21952.t1 MTR_2g078010 45.312 128 70 0 35 162 8 135 6.87e-35 120
MS.gene21952.t1 MTR_8g071040 42.424 132 75 1 35 165 4 135 4.97e-33 115
MS.gene21952.t1 MTR_4g073240 43.089 123 70 0 35 157 4 126 3.77e-32 113
MS.gene21952.t1 MTR_5g075580 41.406 128 75 0 35 162 4 131 5.51e-32 112
MS.gene21952.t1 MTR_3g062450 41.406 128 75 0 35 162 4 131 5.51e-32 112
MS.gene21952.t1 MTR_7g116940 40.458 131 77 1 35 164 4 134 1.24e-31 111
MS.gene21952.t1 MTR_2g078010 40.845 142 70 1 35 162 8 149 1.31e-31 112
MS.gene21952.t1 MTR_1g021150 41.406 128 75 0 35 162 4 131 2.68e-31 110
MS.gene21952.t1 MTR_1g021150 39.231 130 79 0 33 162 10 139 6.76e-29 105
MS.gene21952.t1 MTR_4g108080 46.154 104 56 0 35 138 7 110 8.49e-29 103
MS.gene21952.t1 MTR_7g116940 38.406 138 77 2 35 164 4 141 1.42e-28 103
MS.gene21952.t1 MTR_4g073240 47.872 94 49 0 64 157 4 97 1.87e-28 102
MS.gene21952.t1 MTR_4g108080 51.000 100 48 1 73 171 45 144 2.63e-28 103
MS.gene21952.t1 MTR_5g075580 44.118 102 57 0 61 162 1 102 4.16e-28 101
MS.gene21952.t1 MTR_2g078010 45.545 101 55 0 35 135 8 108 3.67e-27 99.0
MS.gene21952.t1 MTR_4g063490 35.252 139 76 2 49 173 22 160 6.17e-26 97.8
MS.gene21952.t1 MTR_8g085690 37.097 124 78 0 35 158 25 148 2.81e-24 93.6
MS.gene21952.t1 MTR_7g116940 39.623 106 64 0 35 140 4 109 2.95e-24 92.4
MS.gene21952.t1 MTR_3g098780 38.211 123 74 2 36 156 6 128 9.94e-24 92.8
MS.gene21952.t1 MTR_3g098780 37.600 125 76 2 36 158 30 154 1.16e-23 93.2
MS.gene21952.t1 MTR_2g096690 35.115 131 71 2 57 173 30 160 6.66e-23 89.7
MS.gene21952.t1 MTR_2g096690 34.615 130 72 1 57 173 30 159 7.88e-23 89.7
MS.gene21952.t1 MTR_3g110110 36.607 112 70 1 47 157 20 131 2.93e-22 87.8
MS.gene21952.t1 MTR_5g016630 34.932 146 87 3 36 173 8 153 3.10e-21 85.5
MS.gene21952.t1 MTR_4g094872 35.915 142 83 3 36 169 8 149 4.70e-21 84.7
MS.gene21952.t1 MTR_4g106690 36.000 125 75 4 35 157 6 127 3.42e-20 83.2
MS.gene21952.t1 MTR_8g028920 34.400 125 77 4 35 157 6 127 5.21e-18 77.4
MS.gene21952.t1 MTR_5g019480 38.824 85 52 0 73 157 21 105 5.78e-18 76.3
MS.gene21952.t1 MTR_5g019480 31.746 126 85 1 36 161 33 157 8.56e-18 77.0
MS.gene21952.t1 MTR_5g019480 32.787 122 81 1 36 157 36 156 1.07e-17 76.6
MS.gene21952.t1 MTR_7g087590 30.496 141 97 1 32 171 10 150 3.25e-16 74.3
MS.gene21952.t1 MTR_8g098595 36.667 90 55 2 70 157 23 112 6.16e-16 71.6
MS.gene21952.t1 MTR_4g073240 50.820 61 30 0 97 157 4 64 8.19e-16 69.3
MS.gene21952.t1 MTR_8g076490 36.364 99 61 2 36 133 19 116 1.94e-15 70.1
MS.gene21952.t1 MTR_8g028920 36.905 84 51 2 76 157 5 88 3.20e-15 69.3
MS.gene21952.t1 MTR_5g021260 36.364 99 61 2 36 133 19 116 5.31e-14 66.6
MS.gene21952.t1 MTR_2g096690 43.077 65 37 0 57 121 30 94 7.75e-13 62.4
MS.gene21952.t1 MTR_8g023380 32.759 116 69 3 31 141 4 115 5.33e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21952.t1 AT3G20060 85.256 156 23 0 18 173 22 177 1.40e-99 285
MS.gene21952.t1 AT1G50490 85.000 160 24 0 18 177 21 180 9.07e-94 270
MS.gene21952.t1 AT3G20060 87.879 132 16 0 42 173 1 132 2.77e-87 252
MS.gene21952.t1 AT1G14400 44.928 138 75 1 35 171 7 144 4.73e-38 128
MS.gene21952.t1 AT1G14400 44.928 138 75 1 35 171 7 144 4.73e-38 128
MS.gene21952.t1 AT2G02760 44.928 138 75 1 35 171 7 144 5.34e-38 128
MS.gene21952.t1 AT2G02760 44.928 138 75 1 35 171 7 144 5.34e-38 128
MS.gene21952.t1 AT2G02760 44.928 138 75 1 35 171 7 144 5.34e-38 128
MS.gene21952.t1 AT2G02760 44.928 138 75 1 35 171 7 144 5.34e-38 128
MS.gene21952.t1 AT5G62540 45.775 142 75 2 30 169 2 143 5.99e-37 125
MS.gene21952.t1 AT1G78870 46.094 128 69 0 35 162 8 135 1.23e-35 122
MS.gene21952.t1 AT1G16890 45.312 128 70 0 35 162 8 135 5.37e-35 120
MS.gene21952.t1 AT1G16890 46.923 130 67 1 35 162 15 144 3.93e-34 118
MS.gene21952.t1 AT5G56150 42.188 128 74 0 35 162 4 131 2.09e-32 113
MS.gene21952.t1 AT2G16740 39.706 136 81 1 35 169 4 139 3.55e-32 113
MS.gene21952.t1 AT1G64230 41.406 128 75 0 35 162 4 131 7.17e-32 112
MS.gene21952.t1 AT1G64230 41.406 128 75 0 35 162 4 131 7.17e-32 112
MS.gene21952.t1 AT4G27960 40.625 128 76 0 35 162 4 131 1.15e-31 112
MS.gene21952.t1 AT3G08700 40.580 138 80 2 35 170 4 141 1.49e-31 111
MS.gene21952.t1 AT3G08700 40.580 138 80 2 35 170 4 141 1.49e-31 111
MS.gene21952.t1 AT3G08690 41.935 124 72 0 35 158 4 127 3.64e-31 110
MS.gene21952.t1 AT3G08690 41.935 124 72 0 35 158 4 127 3.64e-31 110
MS.gene21952.t1 AT5G41700 39.844 128 77 0 35 162 4 131 5.38e-31 110
MS.gene21952.t1 AT5G41700 39.844 128 77 0 35 162 4 131 5.38e-31 110
MS.gene21952.t1 AT5G41700 39.844 128 77 0 35 162 4 131 5.38e-31 110
MS.gene21952.t1 AT5G53300 39.844 128 77 0 35 162 4 131 5.86e-31 110
MS.gene21952.t1 AT5G53300 39.844 128 77 0 35 162 4 131 5.86e-31 110
MS.gene21952.t1 AT5G53300 39.844 128 77 0 35 162 4 131 5.86e-31 110
MS.gene21952.t1 AT4G27960 40.625 128 76 0 35 162 34 161 1.58e-30 109
MS.gene21952.t1 AT1G16890 51.579 95 46 0 68 162 8 102 1.19e-29 105
MS.gene21952.t1 AT1G78870 51.579 95 46 0 68 162 8 102 1.43e-29 105
MS.gene21952.t1 AT5G41700 39.535 129 77 1 35 162 4 132 6.45e-29 104
MS.gene21952.t1 AT1G64230 38.686 137 75 1 35 162 4 140 1.20e-28 104
MS.gene21952.t1 AT1G78870 45.714 105 57 0 35 139 8 112 3.20e-28 102
MS.gene21952.t1 AT1G64230 42.727 110 62 1 56 164 67 176 4.08e-28 103
MS.gene21952.t1 AT1G64230 42.727 110 62 1 56 164 67 176 4.08e-28 103
MS.gene21952.t1 AT1G78870 51.042 96 46 1 68 162 8 103 6.17e-28 101
MS.gene21952.t1 AT3G13550 37.500 136 85 0 35 170 39 174 3.45e-26 99.0
MS.gene21952.t1 AT1G36340 40.876 137 79 2 26 162 3 137 1.78e-25 96.3
MS.gene21952.t1 AT1G36340 40.876 137 79 2 26 162 3 137 1.78e-25 96.3
MS.gene21952.t1 AT5G50870 40.000 135 73 4 34 166 4 132 1.83e-25 97.1
MS.gene21952.t1 AT5G50870 41.129 124 68 3 34 155 4 124 2.61e-25 97.1
MS.gene21952.t1 AT5G56150 41.000 100 59 0 35 134 4 103 7.21e-24 90.9
MS.gene21952.t1 AT3G55380 33.813 139 78 2 49 173 23 161 4.01e-23 90.5
MS.gene21952.t1 AT3G13550 39.091 110 67 0 35 144 39 148 6.62e-23 90.1
MS.gene21952.t1 AT5G59300 34.351 131 72 2 57 173 30 160 1.00e-22 89.4
MS.gene21952.t1 AT5G41700 38.462 104 64 0 35 138 4 107 1.32e-22 88.6
MS.gene21952.t1 AT3G46460 33.813 139 78 2 49 173 22 160 1.70e-22 89.0
MS.gene21952.t1 AT5G25760 36.290 124 73 2 35 157 13 131 2.50e-22 88.2
MS.gene21952.t1 AT5G25760 36.290 124 73 2 35 157 13 131 2.50e-22 88.2
MS.gene21952.t1 AT5G53300 38.384 99 61 0 35 133 4 102 5.22e-22 85.9
MS.gene21952.t1 AT1G63800 38.400 125 72 4 35 157 6 127 9.25e-22 87.4
MS.gene21952.t1 AT5G41340 37.600 125 73 4 35 157 6 127 6.42e-21 85.1
MS.gene21952.t1 AT3G57870 35.915 142 83 3 36 169 9 150 3.88e-20 82.4
MS.gene21952.t1 AT2G18600 33.333 153 92 5 7 157 11 155 1.70e-19 81.6
MS.gene21952.t1 AT2G18600 33.333 153 92 5 7 157 11 155 1.70e-19 81.6
MS.gene21952.t1 AT1G63800 40.217 92 53 2 68 157 21 112 2.06e-19 80.9
MS.gene21952.t1 AT2G46030 36.000 125 75 4 35 157 6 127 6.06e-19 80.1
MS.gene21952.t1 AT2G46030 36.000 125 75 4 35 157 6 127 6.06e-19 80.1
MS.gene21952.t1 AT2G46030 36.000 125 75 4 35 157 78 199 4.29e-18 79.3
MS.gene21952.t1 AT4G36800 31.061 132 89 2 27 157 24 154 2.24e-17 75.9
MS.gene21952.t1 AT4G36800 31.061 132 89 2 27 157 24 154 2.24e-17 75.9
MS.gene21952.t1 AT5G05080 31.410 156 98 3 18 172 3 150 7.83e-17 75.9
MS.gene21952.t1 AT5G05080 31.410 156 98 3 18 172 3 150 7.83e-17 75.9
MS.gene21952.t1 AT5G05080 31.410 156 98 3 18 172 3 150 7.83e-17 75.9
MS.gene21952.t1 AT5G05080 31.410 156 98 3 18 172 3 150 7.83e-17 75.9
MS.gene21952.t1 AT5G41340 40.000 85 49 2 75 157 8 92 2.81e-16 72.4
MS.gene21952.t1 AT3G24515 32.484 157 86 5 36 173 10 165 3.86e-16 75.5
MS.gene21952.t1 AT2G46030 35.644 101 63 2 59 157 30 130 4.66e-16 72.4
MS.gene21952.t1 AT2G46030 35.644 101 63 2 59 157 30 130 4.66e-16 72.4
MS.gene21952.t1 AT3G46460 33.333 111 60 2 77 173 8 118 6.62e-16 70.5
MS.gene21952.t1 AT3G46460 33.333 111 60 2 77 173 8 118 6.62e-16 70.5
MS.gene21952.t1 AT3G55380 26.590 173 79 3 49 173 23 195 8.23e-16 72.0
MS.gene21952.t1 AT3G24515 35.652 115 65 3 68 173 76 190 9.15e-16 74.3
MS.gene21952.t1 AT1G75440 33.333 111 72 2 24 133 7 116 2.88e-14 67.4
MS.gene21952.t1 AT1G45050 32.432 111 73 2 24 133 7 116 4.06e-14 67.0
MS.gene21952.t1 AT4G36410 34.286 105 67 2 30 133 13 116 9.59e-14 65.9
MS.gene21952.t1 AT5G42990 32.432 111 73 2 24 133 7 116 1.78e-13 65.1
MS.gene21952.t1 AT2G32790 35.246 122 76 1 53 174 51 169 1.29e-12 63.2

Find 39 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACCTTTGGAGCCTTAAA+AGG 0.231384 5.1:+2949455 None:intergenic
GATTGAGTGTCAACGGATTT+AGG 0.260118 5.1:+2950285 None:intergenic
CACAATATATTGTCCTCTTC+AGG 0.336865 5.1:+2949563 None:intergenic
CAGGTTGCAGTCTGAATTGA+TGG 0.339528 5.1:-2949896 MS.gene21952:CDS
TATTTGCTTGGACATTCTTC+AGG 0.340447 5.1:-2949387 MS.gene21952:intron
TGGCAGATGAGTGGAGATTC+TGG 0.360212 5.1:-2949599 MS.gene21952:intron
GTGGTGTCAAATTTGACCTT+TGG 0.405768 5.1:+2949443 None:intergenic
TTGCCATGCAAATCAACATT+GGG 0.409629 5.1:+2949410 None:intergenic
ATCCACAAGCAGCACAGCTT+TGG 0.411990 5.1:-2949072 MS.gene21952:CDS
GTCTGAATTGATGGCTTTGA+TGG 0.419788 5.1:-2949887 MS.gene21952:intron
TTTAGCGGCGAGCTAATGTT+TGG 0.426525 5.1:+2949094 None:intergenic
CACCCCAATGTTGATTTGCA+TGG 0.432591 5.1:-2949413 MS.gene21952:CDS
CTCCAAAGCTGTGCTGCTTG+TGG 0.432715 5.1:+2949070 None:intergenic
GGAGGGAGAGGTTGCATTGA+AGG 0.440179 5.1:+2950309 None:intergenic
ACACTCAATCTGTTCTCAAA+AGG 0.451346 5.1:-2950272 MS.gene21952:intron
AGCCTTAAAAGGATACTCAT+TGG 0.457477 5.1:+2949466 None:intergenic
GCCTTAAAAGGATACTCATT+GGG 0.466815 5.1:+2949467 None:intergenic
TTCTACAGAATACAGGAAGA+TGG 0.481814 5.1:-2948961 MS.gene21952:intron
TTTCAAATTTACCTCCTAGC+AGG 0.485419 5.1:+2949196 None:intergenic
AGCAAAGACACTGTCTTTGA+AGG 0.498680 5.1:-2949518 MS.gene21952:CDS
TGCTGGAAAGGAACAATAGC+AGG 0.507664 5.1:-2949542 MS.gene21952:CDS
GTGTCAACGGATTTAGGCGG+AGG 0.521333 5.1:+2950291 None:intergenic
GACAATATATTGTGCTGGAA+AGG 0.525589 5.1:-2949554 MS.gene21952:CDS
TTTGCATGGCAATATTTGCT+TGG 0.528158 5.1:-2949399 MS.gene21952:CDS
AAGAGGACAATATATTGTGC+TGG 0.531992 5.1:-2949559 MS.gene21952:CDS
ACGGATTTAGGCGGAGGGAG+AGG 0.544685 5.1:+2950297 None:intergenic
GAGAACAGATTGAGTGTCAA+CGG 0.546293 5.1:+2950278 None:intergenic
TTGTCAATTCAAAGCCTGCT+AGG 0.548402 5.1:-2949210 MS.gene21952:CDS
GAGATCAAAGTTTAAGCAGC+AGG 0.549093 5.1:+2948918 None:intergenic
ATTGCCATGCAAATCAACAT+TGG 0.581157 5.1:+2949409 None:intergenic
TGTGCTGCTTGTGGATTTAG+CGG 0.595779 5.1:+2949079 None:intergenic
GTGATTATTTGGCAGATGAG+TGG 0.606809 5.1:-2949608 MS.gene21952:intron
CAGCTTTGGAGCAATCAAGA+AGG 0.619795 5.1:-2949058 MS.gene21952:intron
TCAATTCAAAGCCTGCTAGG+AGG 0.657162 5.1:-2949207 MS.gene21952:intron
TGTCAACGGATTTAGGCGGA+GGG 0.657757 5.1:+2950292 None:intergenic
CATGCAAATCAACATTGGGG+TGG 0.679728 5.1:+2949414 None:intergenic
TGAGTGTCAACGGATTTAGG+CGG 0.688984 5.1:+2950288 None:intergenic
TGCCATGCAAATCAACATTG+GGG 0.689638 5.1:+2949411 None:intergenic
AACATTGGGGTGGAAGCATG+TGG 0.689650 5.1:+2949424 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATTTTTATTTTATTTTTG+TGG - chr5.1:2949352-2949371 MS.gene21952:intron 10.0%
!!! CTTTTTAATTTACTTAATTT+TGG - chr5.1:2949240-2949259 MS.gene21952:CDS 10.0%
!!! GTTTAGTTAATTTAAAATAT+TGG - chr5.1:2949292-2949311 MS.gene21952:intron 10.0%
!!! TTTTAATTTACTTAATTTTG+GGG - chr5.1:2949170-2949189 MS.gene21952:intron 10.0%
!! AAAAATACAATTAACAAACA+GGG + chr5.1:2950152-2950171 None:intergenic 15.0%
!! AATTCATTAATGTGATTATT+TGG - chr5.1:2949560-2949579 MS.gene21952:CDS 15.0%
!! ATTCATTAATGTGATTATTT+GGG - chr5.1:2949561-2949580 MS.gene21952:CDS 15.0%
!! TAAAAATACAATTAACAAAC+AGG + chr5.1:2950153-2950172 None:intergenic 15.0%
!!! AAGTGTTATATTATTGATTT+AGG + chr5.1:2950116-2950135 None:intergenic 15.0%
!!! AATTGATTAATCTGTTTTAT+AGG - chr5.1:2950015-2950034 MS.gene21952:intron 15.0%
!!! AGTTAATTTAAAATATTGGT+AGG - chr5.1:2949296-2949315 MS.gene21952:intron 15.0%
!!! GTTAATTTAAAATATTGGTA+GGG - chr5.1:2949297-2949316 MS.gene21952:intron 15.0%
!!! TGTTGATTAAATTAAAGTTA+GGG - chr5.1:2949206-2949225 MS.gene21952:intron 15.0%
!!! TGTTGATTGAATTAAAATTT+AGG - chr5.1:2949140-2949159 MS.gene21952:intron 15.0%
!!! TTGTTGATTAAATTAAAGTT+AGG - chr5.1:2949205-2949224 MS.gene21952:intron 15.0%
!! AAAAAGTAATTAGATGGTAA+TGG - chr5.1:2949517-2949536 MS.gene21952:CDS 20.0%
!! AAAAGTAATTAGATGGTAAT+GGG - chr5.1:2949518-2949537 MS.gene21952:CDS 20.0%
!! AAAATTGAGTTAGTTAGATT+AGG + chr5.1:2949339-2949358 None:intergenic 20.0%
!! GACTTAAAAAAGTAATTAGA+TGG - chr5.1:2949511-2949530 MS.gene21952:CDS 20.0%
!!! AATCTGTTTTATAGGATAAA+TGG - chr5.1:2950023-2950042 MS.gene21952:intron 20.0%
!!! ACTTAATTTTGGGAATTAAT+TGG - chr5.1:2949251-2949270 MS.gene21952:CDS 20.0%
! TTAAAAGAAATCAAACCCTT+CGG - chr5.1:2949091-2949110 MS.gene21952:CDS 25.0%
!! TAGTTTGTATGAAATCAGTT+GGG - chr5.1:2949633-2949652 MS.gene21952:intron 25.0%
!!! TTAGTTTGTATGAAATCAGT+TGG - chr5.1:2949632-2949651 MS.gene21952:intron 25.0%
AGTTTGTATGAAATCAGTTG+GGG - chr5.1:2949634-2949653 MS.gene21952:intron 30.0%
! ACTTTTCTTCTACAGAATAC+AGG - chr5.1:2950318-2950337 MS.gene21952:CDS 30.0%
! GTCTAAAGCAATGTAATTTG+AGG - chr5.1:2949489-2949508 MS.gene21952:CDS 30.0%
!! ACTTAATTTTGGGGATCTAT+TGG - chr5.1:2949179-2949198 MS.gene21952:intron 30.0%
!! GATAGATTTTGGGGATTAAT+GGG - chr5.1:2949113-2949132 MS.gene21952:intron 30.0%
!! TAGATGGTAATGGGATTATT+TGG - chr5.1:2949527-2949546 MS.gene21952:CDS 30.0%
AAGAGGACAATATATTGTGC+TGG - chr5.1:2949727-2949746 MS.gene21952:intron 35.0%
ACACTCAATCTGTTCTCAAA+AGG - chr5.1:2949014-2949033 MS.gene21952:intron 35.0%
AGCCTTAAAAGGATACTCAT+TGG + chr5.1:2949823-2949842 None:intergenic 35.0%
ATTGCCATGCAAATCAACAT+TGG + chr5.1:2949880-2949899 None:intergenic 35.0%
CACAATATATTGTCCTCTTC+AGG + chr5.1:2949726-2949745 None:intergenic 35.0%
GACAATATATTGTGCTGGAA+AGG - chr5.1:2949732-2949751 MS.gene21952:intron 35.0%
GCCTTAAAAGGATACTCATT+GGG + chr5.1:2949822-2949841 None:intergenic 35.0%
TATTTGCTTGGACATTCTTC+AGG - chr5.1:2949899-2949918 MS.gene21952:intron 35.0%
TTCTACAGAATACAGGAAGA+TGG - chr5.1:2950325-2950344 MS.gene21952:CDS 35.0%
TTGCCATGCAAATCAACATT+GGG + chr5.1:2949879-2949898 None:intergenic 35.0%
TTTCAAATTTACCTCCTAGC+AGG + chr5.1:2950093-2950112 None:intergenic 35.0%
TTTGCATGGCAATATTTGCT+TGG - chr5.1:2949887-2949906 MS.gene21952:intron 35.0%
! AAACCCTTCGGATAGATTTT+GGG - chr5.1:2949103-2949122 MS.gene21952:intron 35.0%
! TCCCAATGAGTATCCTTTTA+AGG - chr5.1:2949818-2949837 MS.gene21952:intron 35.0%
!! GGATAGATTTTGGGGATTAA+TGG - chr5.1:2949112-2949131 MS.gene21952:intron 35.0%
!! TGGCTTTGATGGTAAGAAAT+TGG - chr5.1:2949410-2949429 MS.gene21952:CDS 35.0%
AATCCCCAAAATCTATCCGA+AGG + chr5.1:2949110-2949129 None:intergenic 40.0%
AGCAAAGACACTGTCTTTGA+AGG - chr5.1:2949768-2949787 MS.gene21952:intron 40.0%
ATCCCCAAAATCTATCCGAA+GGG + chr5.1:2949109-2949128 None:intergenic 40.0%
GAGAACAGATTGAGTGTCAA+CGG + chr5.1:2949011-2949030 None:intergenic 40.0%
GTGATTATTTGGCAGATGAG+TGG - chr5.1:2949678-2949697 MS.gene21952:intron 40.0%
TGCCATGCAAATCAACATTG+GGG + chr5.1:2949878-2949897 None:intergenic 40.0%
TTGTCAATTCAAAGCCTGCT+AGG - chr5.1:2950076-2950095 MS.gene21952:intron 40.0%
! AACCCTTCGGATAGATTTTG+GGG - chr5.1:2949104-2949123 MS.gene21952:intron 40.0%
! CAAACCCTTCGGATAGATTT+TGG - chr5.1:2949102-2949121 MS.gene21952:intron 40.0%
! GATTGAGTGTCAACGGATTT+AGG + chr5.1:2949004-2949023 None:intergenic 40.0%
! GTGGTGTCAAATTTGACCTT+TGG + chr5.1:2949846-2949865 None:intergenic 40.0%
!! GTCTGAATTGATGGCTTTGA+TGG - chr5.1:2949399-2949418 MS.gene21952:CDS 40.0%
!! TGTATCTGCTTTTCCTGAAG+AGG - chr5.1:2949710-2949729 MS.gene21952:intron 40.0%
!! TTGACCTTTGGAGCCTTAAA+AGG + chr5.1:2949834-2949853 None:intergenic 40.0%
!!! GTATCCTTTTAAGGCTCCAA+AGG - chr5.1:2949827-2949846 MS.gene21952:intron 40.0%
CAGGTTGCAGTCTGAATTGA+TGG - chr5.1:2949390-2949409 MS.gene21952:CDS 45.0%
CATGCAAATCAACATTGGGG+TGG + chr5.1:2949875-2949894 None:intergenic 45.0%
GTGTCAGTAGCATGTGATGA+TGG - chr5.1:2949933-2949952 MS.gene21952:intron 45.0%
TCAATTCAAAGCCTGCTAGG+AGG - chr5.1:2950079-2950098 MS.gene21952:intron 45.0%
TGCTGGAAAGGAACAATAGC+AGG - chr5.1:2949744-2949763 MS.gene21952:intron 45.0%
TTTAGCGGCGAGCTAATGTT+TGG + chr5.1:2950195-2950214 None:intergenic 45.0%
! TGAGTGTCAACGGATTTAGG+CGG + chr5.1:2949001-2949020 None:intergenic 45.0%
!! CACCCCAATGTTGATTTGCA+TGG - chr5.1:2949873-2949892 MS.gene21952:intron 45.0%
!! CAGCTTTGGAGCAATCAAGA+AGG - chr5.1:2950228-2950247 MS.gene21952:intron 45.0%
!! GTGGGTTGTTGCAATTTTGC+AGG - chr5.1:2949371-2949390 MS.gene21952:intron 45.0%
!! TGTGCTGCTTGTGGATTTAG+CGG + chr5.1:2950210-2950229 None:intergenic 45.0%
!!! AATTTTTATTTTATTTTTGT+GGG - chr5.1:2949353-2949372 MS.gene21952:intron 5.0%
!!! TTTTTAATTTACTTAATTTT+GGG - chr5.1:2949169-2949188 MS.gene21952:intron 5.0%
!!! TTTTTTAATTTACTTAATTT+TGG - chr5.1:2949168-2949187 MS.gene21952:intron 5.0%
ATCCACAAGCAGCACAGCTT+TGG - chr5.1:2950214-2950233 MS.gene21952:intron 50.0%
TGGCAGATGAGTGGAGATTC+TGG - chr5.1:2949687-2949706 MS.gene21952:intron 50.0%
! AACATTGGGGTGGAAGCATG+TGG + chr5.1:2949865-2949884 None:intergenic 50.0%
! TGTCAACGGATTTAGGCGGA+GGG + chr5.1:2948997-2949016 None:intergenic 50.0%
GGAGGGAGAGGTTGCATTGA+AGG + chr5.1:2948980-2948999 None:intergenic 55.0%
! GTGTCAACGGATTTAGGCGG+AGG + chr5.1:2948998-2949017 None:intergenic 55.0%
!! CTCCAAAGCTGTGCTGCTTG+TGG + chr5.1:2950219-2950238 None:intergenic 55.0%
ACGGATTTAGGCGGAGGGAG+AGG + chr5.1:2948992-2949011 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 2948929 2950379 2948929 ID=MS.gene21952
chr5.1 mRNA 2948929 2950379 2948929 ID=MS.gene21952.t1;Parent=MS.gene21952
chr5.1 exon 2950273 2950379 2950273 ID=MS.gene21952.t1.exon1;Parent=MS.gene21952.t1
chr5.1 CDS 2950273 2950379 2950273 ID=cds.MS.gene21952.t1;Parent=MS.gene21952.t1
chr5.1 exon 2949888 2949915 2949888 ID=MS.gene21952.t1.exon2;Parent=MS.gene21952.t1
chr5.1 CDS 2949888 2949915 2949888 ID=cds.MS.gene21952.t1;Parent=MS.gene21952.t1
chr5.1 exon 2949388 2949615 2949388 ID=MS.gene21952.t1.exon3;Parent=MS.gene21952.t1
chr5.1 CDS 2949388 2949615 2949388 ID=cds.MS.gene21952.t1;Parent=MS.gene21952.t1
chr5.1 exon 2949208 2949271 2949208 ID=MS.gene21952.t1.exon4;Parent=MS.gene21952.t1
chr5.1 CDS 2949208 2949271 2949208 ID=cds.MS.gene21952.t1;Parent=MS.gene21952.t1
chr5.1 exon 2949059 2949118 2949059 ID=MS.gene21952.t1.exon5;Parent=MS.gene21952.t1
chr5.1 CDS 2949059 2949118 2949059 ID=cds.MS.gene21952.t1;Parent=MS.gene21952.t1
chr5.1 exon 2948929 2948975 2948929 ID=MS.gene21952.t1.exon6;Parent=MS.gene21952.t1
chr5.1 CDS 2948929 2948975 2948929 ID=cds.MS.gene21952.t1;Parent=MS.gene21952.t1
Gene Sequence

>MS.gene21952

ATGGCTAGCGCTAACGTTCAAACTGAAGCTTCTCAAAGCAACATTGCTCCTTCAATGCAACCTCTCCCTCCGCCTAAATCCGTTGACACTCAATCTGTTCTCAAAAGGTTCGTTTTTTCAATTCAGCATTACAGTTTTTTGAATCTTGTTTTGTTTTTCGATTTAAAAGAAATCAAACCCTTCGGATAGATTTTGGGGATTAATGGGTTTTTGTTGATTGAATTAAAATTTAGGTTTTTTTTTTTAATTTACTTAATTTTGGGGATCTATTGGTTTTTGTTGATTAAATTAAAGTTAGGGTTTTTACTTAACTTTTTAATTTACTTAATTTTGGGAATTAATTGGTTTTTGTTTATTTTTTTAGTTTAGTTAATTTAAAATATTGGTAGGGTTTTCAGTTTGTTTCTCCTAATCTAACTAACTCAATTTTTATTTTATTTTTGTGGGTTGTTGCAATTTTGCAGGTTGCAGTCTGAATTGATGGCTTTGATGGTAAGAAATTGGATCTCTCTTTTTCACTTTCTGTGATCTAATTTTGTGTTAGTTTATCAATTAGTTTTGTCTAAAGCAATGTAATTTGAGGACTTAAAAAAGTAATTAGATGGTAATGGGATTATTTGGAAAAGTGATTAATTCATTAATGTGATTATTTGGGCTCATTTATTTTTTATTCTTGTGTGTGTATGCAATGTAATTTGAGCACTTAGTTTGTATGAAATCAGTTGGGGAAAAGTGATTAATTCATTAATGTGATTATTTGGCAGATGAGTGGAGATTCTGGTGTATCTGCTTTTCCTGAAGAGGACAATATATTGTGCTGGAAAGGAACAATAGCAGGAAGCAAAGACACTGTCTTTGAAGGAACAGAATACAAATTGTCACTTTCATTTCCCAATGAGTATCCTTTTAAGGCTCCAAAGGTCAAATTTGACACCACATGCTTCCACCCCAATGTTGATTTGCATGGCAATATTTGCTTGGACATTCTTCAGGTAATGAATGTTGTGTCAGTAGCATGTGATGATGGTGACATCTTGTTATGTTGTGTCATTTTCAATACAACTATTGCATATTGAGATTATGATTAATTGATTAATCTGTTTTATAGGATAAATGGTCATCTGCTTATGATGTTAGAACAATTCTTTTGTCAATTCAAAGCCTGCTAGGAGGTAAATTTGAAACCTAAATCAATAATATAACACTTTTATTTTGTTCTTCCCTGTTTGTTAATTGTATTTTTACTTCTTTCATAATTTAGAGCCAAACATTAGCTCGCCGCTAAATCCACAAGCAGCACAGCTTTGGAGCAATCAAGAAGGTAAATAAACATAATTGATATTTTGTAGAGTATATCTTCTGAAAGATGAACTGATTTTGCTGTGTTCTACTTTTCTTCTACAGAATACAGGAAGATGGTTGAGAAGTTGTACAAAGATCCTGCTGCTTAA
Protein sequence

>MS.gene21952.t1

MASANVQTEASQSNIAPSMQPLPPPKSVDTQSVLKRLQSELMALMMSGDSGVSAFPEEDNILCWKGTIAGSKDTVFEGTEYKLSLSFPNEYPFKAPKVKFDTTCFHPNVDLHGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNPQAAQLWSNQEEYRKMVEKLYKDPAA