AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene019627

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019627.t1 MTR_8g030930 88.471 399 42 2 1 395 1 399 0.0 695
MS.gene019627.t1 MTR_3g438050 63.131 396 143 3 1 394 1 395 0.0 515
MS.gene019627.t1 MTR_5g063430 70.418 311 87 1 1 311 1 306 3.50e-167 470
MS.gene019627.t1 MTR_1g088160 42.216 379 205 7 24 394 34 406 1.62e-99 302
MS.gene019627.t1 MTR_2g058670 37.913 393 232 6 6 390 6 394 6.16e-97 295
MS.gene019627.t1 MTR_2g058840 37.531 397 226 6 6 390 6 392 2.50e-96 294
MS.gene019627.t1 MTR_2g058640 37.755 392 232 6 7 390 7 394 1.45e-92 284
MS.gene019627.t1 MTR_4g083660 40.642 374 210 7 25 394 9 374 6.98e-90 276
MS.gene019627.t1 MTR_2g049400 39.286 392 224 8 5 394 12 391 7.66e-89 274
MS.gene019627.t1 MTR_2g435640 37.766 376 225 6 23 393 51 422 6.49e-87 270
MS.gene019627.t1 MTR_1g052315 40.779 385 212 10 8 382 46 424 5.53e-86 268
MS.gene019627.t1 MTR_5g097860 43.125 320 176 4 21 337 21 337 6.67e-86 265
MS.gene019627.t1 MTR_7g091850 40.793 353 198 7 18 364 69 416 6.39e-85 266
MS.gene019627.t1 MTR_4g120320 36.340 377 229 6 23 393 50 421 3.82e-82 258
MS.gene019627.t1 MTR_7g091840 41.250 320 177 6 23 335 63 378 3.23e-80 251
MS.gene019627.t1 MTR_6g028080 39.024 369 198 9 25 377 30 387 2.57e-79 249
MS.gene019627.t1 MTR_2g060350 38.624 378 214 10 24 390 73 443 3.65e-79 251
MS.gene019627.t1 MTR_2g103410 36.605 377 226 8 24 393 58 428 5.13e-79 250
MS.gene019627.t1 MTR_4g084050 40.533 375 198 9 4 364 2 365 8.96e-79 248
MS.gene019627.t1 MTR_8g009810 36.896 393 236 8 5 393 15 399 9.66e-79 248
MS.gene019627.t1 MTR_3g030540 40.845 355 199 7 15 364 60 408 1.89e-76 243
MS.gene019627.t1 MTR_8g065030 34.133 375 234 7 24 392 64 431 3.67e-76 243
MS.gene019627.t1 MTR_3g084380 40.115 349 189 8 25 364 21 358 1.26e-74 238
MS.gene019627.t1 MTR_3g054320 38.070 373 200 9 11 366 7 365 1.38e-72 232
MS.gene019627.t1 MTR_3g084360 37.931 377 212 9 6 371 3 368 4.70e-72 231
MS.gene019627.t1 MTR_5g043350 37.808 365 204 9 4 354 2 357 2.02e-71 229
MS.gene019627.t1 MTR_2g029170 39.296 341 195 8 58 394 29 361 1.23e-70 226
MS.gene019627.t1 MTR_8g010110 36.364 374 219 6 7 377 13 370 2.04e-70 226
MS.gene019627.t1 MTR_4g094715 33.938 386 236 8 24 395 64 444 4.57e-70 228
MS.gene019627.t1 MTR_8g010080 35.356 379 223 7 5 377 7 369 6.16e-70 225
MS.gene019627.t1 MTR_8g010070 35.156 384 229 8 5 384 11 378 5.72e-69 223
MS.gene019627.t1 MTR_6g005630 35.185 378 223 11 5 370 17 384 1.17e-68 223
MS.gene019627.t1 MTR_1g086390 34.987 383 229 9 24 391 80 457 4.16e-68 223
MS.gene019627.t1 MTR_5g033680 33.943 383 236 9 21 392 55 431 1.33e-66 219
MS.gene019627.t1 MTR_7g110050 34.367 387 232 11 24 394 66 446 1.98e-66 218
MS.gene019627.t1 MTR_0157s0060 38.873 355 190 11 29 372 2 340 2.03e-66 215
MS.gene019627.t1 MTR_4g034020 38.873 355 190 11 29 372 2 340 2.03e-66 215
MS.gene019627.t1 MTR_2g100040 35.376 359 181 6 7 363 8 317 4.93e-66 214
MS.gene019627.t1 MTR_2g103650 32.984 382 243 7 5 377 8 385 5.27e-66 215
MS.gene019627.t1 MTR_8g010090 34.734 357 214 6 24 377 30 370 3.99e-65 213
MS.gene019627.t1 MTR_8g008765 35.694 353 205 6 5 350 7 344 7.75e-65 211
MS.gene019627.t1 MTR_1g012390 32.737 391 250 9 6 391 9 391 4.03e-61 202
MS.gene019627.t1 MTR_8g010040 34.551 356 213 7 25 377 32 370 6.59e-61 202
MS.gene019627.t1 MTR_5g034090 33.244 373 214 8 24 366 120 487 1.04e-60 205
MS.gene019627.t1 MTR_1g013730 32.552 384 232 8 6 384 8 369 3.11e-59 197
MS.gene019627.t1 MTR_7g035415 33.645 321 188 8 5 310 10 320 5.76e-57 190
MS.gene019627.t1 MTR_1g013740 30.808 396 246 8 5 394 15 388 6.59e-57 191
MS.gene019627.t1 MTR_8g010050 40.580 276 153 4 5 276 11 279 1.29e-56 189
MS.gene019627.t1 MTR_2g103410 36.727 275 167 4 24 292 58 331 1.33e-56 189
MS.gene019627.t1 MTR_8g065010 31.367 373 220 8 25 366 107 474 2.40e-55 190
MS.gene019627.t1 MTR_7g113910 35.987 314 161 10 85 393 74 352 1.37e-54 185
MS.gene019627.t1 MTR_2g032710 32.493 357 221 7 24 366 66 416 1.76e-54 187
MS.gene019627.t1 MTR_6g005630 33.069 378 208 13 5 370 17 361 2.22e-54 185
MS.gene019627.t1 MTR_8g077320 34.194 310 160 6 15 310 23 302 6.54e-48 167
MS.gene019627.t1 MTR_5g034090 33.559 295 166 5 24 288 120 414 8.27e-47 165
MS.gene019627.t1 MTR_8g008700 34.375 288 170 5 84 367 14 286 4.13e-44 155
MS.gene019627.t1 MTR_8g031180 31.525 295 166 6 85 372 15 280 1.44e-35 132
MS.gene019627.t1 MTR_7g072360 58.182 110 24 1 167 276 41 128 3.88e-32 118
MS.gene019627.t1 MTR_7g072370 75.806 62 15 0 221 282 1 62 1.78e-24 95.5
MS.gene019627.t1 MTR_7g078680 23.116 398 264 10 26 391 56 443 9.86e-18 85.1
MS.gene019627.t1 MTR_4g124980 23.325 403 265 12 24 393 73 464 4.70e-17 83.2
MS.gene019627.t1 MTR_2g042740 23.325 403 265 13 24 393 55 446 2.69e-16 80.9
MS.gene019627.t1 MTR_8g098840 22.864 398 265 11 26 391 45 432 3.06e-15 77.4
MS.gene019627.t1 MTR_1g100050 23.350 394 252 10 27 384 52 431 1.11e-14 75.9
MS.gene019627.t1 MTR_8g028725 22.892 415 276 11 7 391 43 443 1.95e-14 75.1
MS.gene019627.t1 MTR_1g100060 22.727 396 252 12 27 384 95 474 3.49e-14 74.3
MS.gene019627.t1 MTR_1g100040 23.096 394 253 10 27 384 58 437 4.41e-13 70.9
MS.gene019627.t1 MTR_1g099850 22.500 440 253 12 8 384 22 436 1.75e-12 68.9
MS.gene019627.t1 MTR_4g120730 22.074 376 241 12 26 364 41 401 1.84e-12 68.9
MS.gene019627.t1 MTR_3g449590 23.040 421 272 12 15 393 37 447 2.78e-12 68.2
MS.gene019627.t1 MTR_5g090160 22.328 421 276 13 11 393 28 435 4.83e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019627.t1 AT1G78400 51.206 373 181 1 23 395 33 404 1.88e-135 393
MS.gene019627.t1 AT2G15450 49.490 392 193 4 7 394 13 403 1.49e-131 384
MS.gene019627.t1 AT1G17150 47.558 389 201 2 8 394 14 401 5.49e-131 382
MS.gene019627.t1 AT1G43080 49.618 393 189 6 9 394 13 403 3.82e-130 380
MS.gene019627.t1 AT1G43090 50.130 385 185 5 9 387 13 396 1.57e-129 380
MS.gene019627.t1 AT2G15460 49.227 388 192 4 7 390 13 399 1.34e-128 376
MS.gene019627.t1 AT2G33160 47.643 403 200 5 1 393 1 402 1.67e-128 384
MS.gene019627.t1 AT2G15470 48.980 392 195 4 7 394 13 403 2.24e-128 375
MS.gene019627.t1 AT4G13760 48.223 394 195 5 5 394 15 403 1.44e-127 373
MS.gene019627.t1 AT1G43100 50.259 386 183 6 9 387 13 396 2.07e-126 372
MS.gene019627.t1 AT2G40310 47.980 396 200 5 4 394 9 403 2.98e-125 367
MS.gene019627.t1 AT2G26620 48.052 385 197 3 12 394 18 401 9.95e-123 361
MS.gene019627.t1 AT3G07820 38.422 393 235 3 1 393 1 386 1.27e-100 304
MS.gene019627.t1 AT5G48140 39.695 393 225 4 1 393 6 386 1.94e-100 304
MS.gene019627.t1 AT3G07840 40.464 388 220 5 6 393 11 387 9.78e-100 302
MS.gene019627.t1 AT2G43890 42.091 373 204 8 25 394 29 392 1.05e-98 299
MS.gene019627.t1 AT4G18180 40.260 385 214 6 24 393 38 421 1.79e-98 300
MS.gene019627.t1 AT3G07830 38.287 397 231 4 1 393 1 387 4.52e-96 293
MS.gene019627.t1 AT1G05650 38.520 392 232 6 5 394 8 392 3.44e-95 290
MS.gene019627.t1 AT1G80170 39.791 382 218 7 20 394 48 424 1.78e-94 290
MS.gene019627.t1 AT1G05660 37.659 393 236 6 4 394 7 392 9.62e-92 281
MS.gene019627.t1 AT3G14040 40.209 383 223 4 15 395 61 439 2.96e-91 282
MS.gene019627.t1 AT3G07850 40.483 373 216 4 25 395 70 438 2.26e-89 277
MS.gene019627.t1 AT2G43880 39.084 371 218 5 25 394 31 394 4.48e-88 272
MS.gene019627.t1 AT2G43870 39.846 389 219 10 6 390 4 381 5.06e-88 271
MS.gene019627.t1 AT3G59850 40.160 376 213 8 23 394 21 388 9.34e-87 268
MS.gene019627.t1 AT3G07970 40.053 377 216 7 19 390 65 436 1.04e-86 270
MS.gene019627.t1 AT3G59850 40.160 376 213 8 23 394 65 432 2.62e-86 269
MS.gene019627.t1 AT2G41850 39.370 381 218 8 15 389 58 431 4.14e-85 266
MS.gene019627.t1 AT3G57510 40.000 375 213 8 8 376 52 420 7.54e-85 265
MS.gene019627.t1 AT1G02790 36.649 382 221 5 1 370 16 388 3.92e-83 260
MS.gene019627.t1 AT2G43860 40.563 355 195 9 25 370 33 380 1.25e-79 251
MS.gene019627.t1 AT1G65570 36.000 400 237 12 5 394 7 397 4.09e-75 239
MS.gene019627.t1 AT2G43880 41.869 289 162 4 25 312 31 314 5.23e-75 236
MS.gene019627.t1 AT4G35670 36.939 379 222 8 23 395 22 389 9.66e-75 238
MS.gene019627.t1 AT1G70500 33.333 375 237 7 24 392 66 433 7.00e-74 238
MS.gene019627.t1 AT1G23460 33.067 375 238 7 24 392 42 409 5.46e-72 232
MS.gene019627.t1 AT3G26610 36.827 353 209 7 24 368 66 412 5.71e-72 233
MS.gene019627.t1 AT1G23460 33.067 375 238 7 24 392 66 433 9.97e-72 232
MS.gene019627.t1 AT3G15720 36.785 367 214 7 25 384 23 378 2.15e-71 231
MS.gene019627.t1 AT3G15720 36.785 367 214 7 25 384 24 379 2.53e-71 231
MS.gene019627.t1 AT5G17200 33.411 428 232 9 1 388 1 415 5.63e-70 226
MS.gene019627.t1 AT1G02460 34.625 387 228 10 24 391 88 468 8.30e-69 225
MS.gene019627.t1 AT3G15720 36.512 367 212 8 25 384 23 375 2.70e-68 223
MS.gene019627.t1 AT5G27530 36.963 349 194 8 23 364 51 380 4.23e-68 223
MS.gene019627.t1 AT5G14650 35.714 378 227 12 25 395 51 419 4.29e-67 219
MS.gene019627.t1 AT1G80170 41.509 265 148 4 20 278 48 311 4.55e-67 216
MS.gene019627.t1 AT4G32380 34.080 402 242 9 5 393 3 394 4.70e-67 219
MS.gene019627.t1 AT5G44840 33.081 396 246 8 6 395 8 390 2.90e-66 216
MS.gene019627.t1 AT4G01890 33.516 364 215 11 24 367 64 420 4.65e-64 212
MS.gene019627.t1 AT1G80140 36.232 345 200 8 58 393 2 335 8.08e-64 207
MS.gene019627.t1 AT1G10640 31.000 400 238 7 23 392 117 508 1.86e-63 212
MS.gene019627.t1 AT1G60590 31.467 375 219 7 25 366 123 492 6.01e-62 208
MS.gene019627.t1 AT1G56710 33.161 386 238 8 24 394 47 427 7.96e-62 205
MS.gene019627.t1 AT1G48100 33.427 356 219 8 24 366 79 429 1.36e-61 206
MS.gene019627.t1 AT3G15720 36.308 325 190 6 66 384 2 315 3.69e-60 200
MS.gene019627.t1 AT4G32375 35.417 288 176 3 99 385 8 286 2.36e-57 193
MS.gene019627.t1 AT4G32370 36.923 325 173 12 5 310 11 322 7.09e-57 190
MS.gene019627.t1 AT5G39910 30.456 417 204 8 1 390 6 363 1.33e-56 190
MS.gene019627.t1 AT4G35670 36.519 293 173 5 104 395 2 282 3.07e-54 181
MS.gene019627.t1 AT5G44830 34.405 311 180 6 59 364 10 301 3.23e-54 182
MS.gene019627.t1 AT4G32380 30.500 400 218 8 5 393 3 353 1.04e-53 183
MS.gene019627.t1 AT5G14650 37.066 259 154 7 25 278 51 305 3.07e-48 166
MS.gene019627.t1 AT1G19170 23.342 407 261 12 24 393 86 478 2.83e-17 84.0
MS.gene019627.t1 AT4G23820 24.297 391 241 11 15 367 31 404 3.60e-17 83.2
MS.gene019627.t1 AT3G48950 21.921 406 261 11 27 393 47 435 2.97e-15 77.8
MS.gene019627.t1 AT3G61490 21.968 437 276 12 6 391 11 433 1.01e-14 75.9
MS.gene019627.t1 AT3G61490 21.968 437 276 12 6 391 11 433 1.01e-14 75.9
MS.gene019627.t1 AT3G61490 21.968 437 276 12 6 391 11 433 1.01e-14 75.9
MS.gene019627.t1 AT3G61490 21.968 437 276 12 6 391 26 448 1.17e-14 75.9
MS.gene019627.t1 AT3G61490 21.968 437 276 12 6 391 124 546 2.61e-14 75.1
MS.gene019627.t1 AT5G41870 22.760 413 270 12 15 393 35 432 1.31e-13 72.4
MS.gene019627.t1 AT3G62110 23.544 412 252 13 15 384 34 424 1.77e-13 72.0
MS.gene019627.t1 AT3G62110 23.544 412 252 13 15 384 34 424 1.77e-13 72.0
MS.gene019627.t1 AT2G23900 24.150 294 200 8 113 393 163 446 9.38e-12 67.0
MS.gene019627.t1 AT4G23500 22.362 398 267 11 26 391 69 456 5.15e-11 64.7

Find 70 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGTCATTTCGTATTATT+TGG 0.045891 8.1:-71985906 MS.gene019627:CDS
ATTCATTTAAGACTGATAAT+TGG 0.248897 8.1:-71986811 MS.gene019627:CDS
TTAAGTATATATGTCCCTTC+TGG 0.254708 8.1:+71986914 None:intergenic
ATCTGTGTTGGGGCTATCAT+TGG 0.279672 8.1:+71985842 None:intergenic
AACACAGATGGTATCAAAAT+AGG 0.286858 8.1:-71985828 MS.gene019627:CDS
TTATCAGTCTTAAATGAATA+TGG 0.288964 8.1:+71986815 None:intergenic
TATCCTCCACTCTAATGTTT+TGG 0.298712 8.1:+71985235 None:intergenic
GATATTAATTTGCGGCCTTC+TGG 0.303523 8.1:-71985216 MS.gene019627:CDS
TCTGTGTTGGGGCTATCATT+GGG 0.307341 8.1:+71985843 None:intergenic
CAATGTGTGTTTATTTGTTA+AGG 0.321877 8.1:-71985175 MS.gene019627:CDS
TACTAGGAATGTTCGAATTT+CGG 0.331851 8.1:-71985733 MS.gene019627:CDS
CTTAAGGAAGTTGTATTTAG+TGG 0.357373 8.1:-71986896 MS.gene019627:CDS
GTTAAGAATTGTACCTTTAG+TGG 0.375278 8.1:-71985627 MS.gene019627:CDS
CCCCTTCTTGCATAAAATAG+TGG 0.382661 8.1:-71985128 None:intergenic
ACGTCCTCCGTGCACCAAAA+AGG 0.391567 8.1:-71985469 MS.gene019627:intron
AAAGTCATTTCGTATTATTT+GGG 0.397273 8.1:-71985905 MS.gene019627:CDS
TGGATGAACTTTAAAATTGA+AGG 0.400013 8.1:-71986860 MS.gene019627:CDS
TTGCTATGCTCTCGGGTACT+AGG 0.400268 8.1:-71985749 MS.gene019627:CDS
CATTGAAAAGCTTGTATTGA+CGG 0.403752 8.1:+71987155 None:intergenic
AATGTGTGTTTATTTGTTAA+GGG 0.428144 8.1:-71985174 MS.gene019627:CDS
ACACAGATGGTATCAAAATA+GGG 0.438610 8.1:-71985827 MS.gene019627:CDS
AGAAGGGACATATATACTTA+AGG 0.451751 8.1:-71986912 MS.gene019627:CDS
TTTGTTAAGGGTGCTAGTTA+TGG 0.458228 8.1:-71985162 MS.gene019627:CDS
GGAATTAGTGTCGGAAGTCT+TGG 0.461747 8.1:-71985687 MS.gene019627:CDS
GTCTCAATTGAAGAAAACTT+TGG 0.463231 8.1:-71985571 MS.gene019627:CDS
TAAGTATATATGTCCCTTCT+GGG 0.466667 8.1:+71986915 None:intergenic
ACCTTTAGTGGCACAAGTAA+TGG 0.469662 8.1:-71985615 MS.gene019627:CDS
ATCAAAATAGGGAGGTCAAA+TGG 0.473186 8.1:-71985816 MS.gene019627:CDS
CTGTGTTGGGGCTATCATTG+GGG 0.485147 8.1:+71985844 None:intergenic
GAAAATTACCACTCTATTGT+CGG 0.485190 8.1:+71987099 None:intergenic
GGTGGAGGTGGCAGATTGGA+TGG 0.495038 8.1:-71986755 MS.gene019627:CDS
GATTGCATTGCTATGCTCTC+GGG 0.503203 8.1:-71985756 MS.gene019627:CDS
TCAAGTGTGAACATTGGAAC+CGG 0.504301 8.1:-71985783 MS.gene019627:CDS
TAATCTTGATCAATGACAAT+GGG 0.504846 8.1:+71985498 None:intergenic
ATGGTCAAGGATCTTCTGCT+TGG 0.509723 8.1:-71986736 MS.gene019627:CDS
TGATTGCATTGCTATGCTCT+CGG 0.518127 8.1:-71985757 MS.gene019627:CDS
AATTATGGTGCACGTTCTGA+TGG 0.520827 8.1:-71987127 MS.gene019627:CDS
AAATTGACTGTTGGTGGAGG+TGG 0.527277 8.1:-71986767 MS.gene019627:CDS
ACCATTACTTGTGCCACTAA+AGG 0.530125 8.1:+71985614 None:intergenic
AAGTTCATCCAAGCATTGCA+TGG 0.537683 8.1:+71986872 None:intergenic
TTAAATGAATATGGATCACT+TGG 0.537771 8.1:+71986824 None:intergenic
TTAGTGGTCCATGCAATGCT+TGG 0.539885 8.1:-71986880 MS.gene019627:CDS
GAGTGGAGGATATTAATTTG+CGG 0.539974 8.1:-71985224 MS.gene019627:CDS
AAATTTGACTTCATCGCCAA+TGG 0.546749 8.1:-71985963 MS.gene019627:CDS
AATATTTCAAGTGTGAACAT+TGG 0.546960 8.1:-71985789 MS.gene019627:CDS
TGTTGGTGGAGGTGGCAGAT+TGG 0.550018 8.1:-71986759 MS.gene019627:CDS
GTTAACAAATTGACTGTTGG+TGG 0.553903 8.1:-71986773 MS.gene019627:CDS
GGAAGTCTTGGTAAGAATGA+AGG 0.566088 8.1:-71985675 MS.gene019627:CDS
AACAAATTGACTGTTGGTGG+AGG 0.571472 8.1:-71986770 MS.gene019627:CDS
CATGGAGTGATGCATGCAGA+TGG 0.579101 8.1:-71986961 MS.gene019627:CDS
TGTGTTGGGGCTATCATTGG+GGG 0.592002 8.1:+71985845 None:intergenic
CATGTGATGGACATAACCAT+TGG 0.593698 8.1:+71985947 None:intergenic
AGTGATGCATGCAGATGGAA+TGG 0.594601 8.1:-71986956 MS.gene019627:CDS
CATAGCAATGCAATCATCAC+CGG 0.595285 8.1:+71985764 None:intergenic
TGTGTATGAAGATATTGTGA+TGG 0.595589 8.1:-71985535 MS.gene019627:CDS
ATAATCTTGATCAATGACAA+TGG 0.600281 8.1:+71985497 None:intergenic
AATGATAGCCCCAACACAGA+TGG 0.606825 8.1:-71985840 MS.gene019627:CDS
GGTGGCAGATTGGATGGTCA+AGG 0.607840 8.1:-71986749 MS.gene019627:CDS
ACCATGCCAAAACATTAGAG+TGG 0.613487 8.1:-71985241 MS.gene019627:CDS
TGGTGTTAGAATCAAATCGT+GGG 0.618282 8.1:-71985595 MS.gene019627:CDS
CCTGGTCATGGAATTAGTGT+CGG 0.621889 8.1:-71985696 MS.gene019627:CDS
CCGACACTAATTCCATGACC+AGG 0.625972 8.1:+71985696 None:intergenic
TATGTTAACAAATTGACTGT+TGG 0.627069 8.1:-71986776 MS.gene019627:CDS
CAATGGTTATGTCCATCACA+TGG 0.630146 8.1:-71985946 MS.gene019627:CDS
ATGGTGTTAGAATCAAATCG+TGG 0.632554 8.1:-71985596 MS.gene019627:CDS
TGTTGATAGATTCCATGTGA+TGG 0.635321 8.1:+71985934 None:intergenic
ATGCCAAAACATTAGAGTGG+AGG 0.635960 8.1:-71985238 MS.gene019627:CDS
AAGTGTACAAATAAGCAATG+TGG 0.668805 8.1:-71985331 MS.gene019627:CDS
TGGCAATACCGACAATAGAG+TGG 0.681148 8.1:-71987107 MS.gene019627:intron
CAGATGGTATCAAAATAGGG+AGG 0.716988 8.1:-71985824 MS.gene019627:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATTCTAATATACAAA+AGG - chr8.1:71986195-71986214 MS.gene019627:intron 10.0%
!! TTAACAAAAAAAAAACGATA+AGG - chr8.1:71985928-71985947 MS.gene019627:CDS 15.0%
!!! TATCATGATTTTGATTTTAA+TGG + chr8.1:71986065-71986084 None:intergenic 15.0%
!! AAAGTCATTTCGTATTATTT+GGG - chr8.1:71986437-71986456 MS.gene019627:intron 20.0%
!! ATATAAAATGAACTTACAGT+AGG + chr8.1:71985674-71985693 None:intergenic 20.0%
!! CTAATTTCTTAACTAAATAG+TGG + chr8.1:71986158-71986177 None:intergenic 20.0%
!! GAGAGGAAAAAAAATATAAA+TGG - chr8.1:71985813-71985832 MS.gene019627:CDS 20.0%
!! TGAAATAAATCATCAAACAT+TGG - chr8.1:71986095-71986114 MS.gene019627:intron 20.0%
!! TTATCAGTCTTAAATGAATA+TGG + chr8.1:71985530-71985549 None:intergenic 20.0%
!!! AATGTGTGTTTATTTGTTAA+GGG - chr8.1:71987168-71987187 MS.gene019627:CDS 20.0%
!!! AGTTTGTTATTTTCTTATGA+AGG - chr8.1:71985346-71985365 MS.gene019627:intron 20.0%
!!! ATATATTTTGTGGATATGTT+TGG - chr8.1:71986346-71986365 MS.gene019627:intron 20.0%
!!! ATTCATTTAAGACTGATAAT+TGG - chr8.1:71985531-71985550 MS.gene019627:CDS 20.0%
! AAACTTATAAAAGACTAAGC+TGG + chr8.1:71986940-71986959 None:intergenic 25.0%
! AACTTATAAAAGACTAAGCT+GGG + chr8.1:71986939-71986958 None:intergenic 25.0%
! ATAATCTTGATCAATGACAA+TGG + chr8.1:71986848-71986867 None:intergenic 25.0%
! CAAAGTCATTTCGTATTATT+TGG - chr8.1:71986436-71986455 MS.gene019627:intron 25.0%
! TAATCTTGATCAATGACAAT+GGG + chr8.1:71986847-71986866 None:intergenic 25.0%
! TACAAAAATATTAGAGGAAG+TGG - chr8.1:71987036-71987055 MS.gene019627:intron 25.0%
! TATGTTAACAAATTGACTGT+TGG - chr8.1:71985566-71985585 MS.gene019627:CDS 25.0%
! TTAAATGAATATGGATCACT+TGG + chr8.1:71985521-71985540 None:intergenic 25.0%
!! AATACATGTACATACCTTTT+TGG + chr8.1:71986890-71986909 None:intergenic 25.0%
!! AATATTTCAAGTGTGAACAT+TGG - chr8.1:71986553-71986572 MS.gene019627:intron 25.0%
!! ATTACTAAAGCTCATTTTGT+TGG - chr8.1:71985895-71985914 MS.gene019627:CDS 25.0%
!! CAATGTGTGTTTATTTGTTA+AGG - chr8.1:71987167-71987186 MS.gene019627:CDS 25.0%
!! CTTTTCAATGTGAAGAATTA+TGG - chr8.1:71985200-71985219 MS.gene019627:CDS 25.0%
!! GTCATGTTTAATTAGTTTCT+AGG - chr8.1:71986025-71986044 MS.gene019627:intron 25.0%
!! TCTGACAATAATTTTGTGTA+GGG - chr8.1:71986982-71987001 MS.gene019627:intron 25.0%
!! TTACTAAAGCTCATTTTGTT+GGG - chr8.1:71985896-71985915 MS.gene019627:CDS 25.0%
!! TTCTGACAATAATTTTGTGT+AGG - chr8.1:71986981-71987000 MS.gene019627:intron 25.0%
!!! ATCGTTTTATTCAAAATGAG+AGG - chr8.1:71985796-71985815 MS.gene019627:CDS 25.0%
!!! TGATTTTGATTTTAATGGTG+AGG + chr8.1:71986060-71986079 None:intergenic 25.0%
!!! TGGATGAACTTTAAAATTGA+AGG - chr8.1:71985482-71985501 MS.gene019627:CDS 25.0%
AAAAACGATAAGGTAGAGTA+TGG - chr8.1:71985938-71985957 MS.gene019627:CDS 30.0%
AAAATGCGAATGAATGAACA+TGG + chr8.1:71985268-71985287 None:intergenic 30.0%
AACACAGATGGTATCAAAAT+AGG - chr8.1:71986514-71986533 MS.gene019627:intron 30.0%
AAGTGTACAAATAAGCAATG+TGG - chr8.1:71987011-71987030 MS.gene019627:intron 30.0%
ACACAGATGGTATCAAAATA+GGG - chr8.1:71986515-71986534 MS.gene019627:intron 30.0%
AGAAGGGACATATATACTTA+AGG - chr8.1:71985430-71985449 MS.gene019627:intron 30.0%
AGTATGGAAAAAGTAGAGAT+TGG - chr8.1:71985954-71985973 MS.gene019627:CDS 30.0%
CATTGAAAAGCTTGTATTGA+CGG + chr8.1:71985190-71985209 None:intergenic 30.0%
CTTAAGGAAGTTGTATTTAG+TGG - chr8.1:71985446-71985465 MS.gene019627:intron 30.0%
GAAAATTACCACTCTATTGT+CGG + chr8.1:71985246-71985265 None:intergenic 30.0%
GTCTCAATTGAAGAAAACTT+TGG - chr8.1:71986771-71986790 MS.gene019627:CDS 30.0%
GTGGCTTACAAAAATATTAG+AGG - chr8.1:71987030-71987049 MS.gene019627:intron 30.0%
GTTAAGAATTGTACCTTTAG+TGG - chr8.1:71986715-71986734 MS.gene019627:CDS 30.0%
TAAGTATATATGTCCCTTCT+GGG + chr8.1:71985430-71985449 None:intergenic 30.0%
TACTAGGAATGTTCGAATTT+CGG - chr8.1:71986609-71986628 MS.gene019627:intron 30.0%
TATACCCTATGAAATTGATG+TGG - chr8.1:71985719-71985738 MS.gene019627:CDS 30.0%
TGTGTATGAAGATATTGTGA+TGG - chr8.1:71986807-71986826 MS.gene019627:CDS 30.0%
TTAAGTATATATGTCCCTTC+TGG + chr8.1:71985431-71985450 None:intergenic 30.0%
! CTGACAATAATTTTGTGTAG+GGG - chr8.1:71986983-71987002 MS.gene019627:intron 30.0%
! GAGATAGAGTGTTTGTATTT+CGG + chr8.1:71985981-71986000 None:intergenic 30.0%
! TGACAATAATTTTGTGTAGG+GGG - chr8.1:71986984-71987003 MS.gene019627:intron 30.0%
!! ATTTTGATACCATCTGTGTT+GGG + chr8.1:71986514-71986533 None:intergenic 30.0%
!!! TATTTTGATACCATCTGTGT+TGG + chr8.1:71986515-71986534 None:intergenic 30.0%
!!! TGAAGGCATTTTTAAAAGCA+TGG - chr8.1:71985363-71985382 MS.gene019627:intron 30.0%
ATCAAAATAGGGAGGTCAAA+TGG - chr8.1:71986526-71986545 MS.gene019627:intron 35.0%
ATCTGCCACATCAATTTCAT+AGG + chr8.1:71985727-71985746 None:intergenic 35.0%
GGTCAAAAGAACAAGCATTA+AGG + chr8.1:71986137-71986156 None:intergenic 35.0%
GTTAACAAATTGACTGTTGG+TGG - chr8.1:71985569-71985588 MS.gene019627:CDS 35.0%
TCTGCCACATCAATTTCATA+GGG + chr8.1:71985726-71985745 None:intergenic 35.0%
! AAATTTGACTTCATCGCCAA+TGG - chr8.1:71986379-71986398 MS.gene019627:intron 35.0%
! ATGGTGTTAGAATCAAATCG+TGG - chr8.1:71986746-71986765 MS.gene019627:CDS 35.0%
! CTCATTTTGTTGGGAATTGA+GGG - chr8.1:71985905-71985924 MS.gene019627:CDS 35.0%
! GAGTGGAGGATATTAATTTG+CGG - chr8.1:71987118-71987137 MS.gene019627:CDS 35.0%
! TATCCTCCACTCTAATGTTT+TGG + chr8.1:71987110-71987129 None:intergenic 35.0%
! TGGTGTTAGAATCAAATCGT+GGG - chr8.1:71986747-71986766 MS.gene019627:CDS 35.0%
! TGTTGATAGATTCCATGTGA+TGG + chr8.1:71986411-71986430 None:intergenic 35.0%
! TTTTGATACCATCTGTGTTG+GGG + chr8.1:71986513-71986532 None:intergenic 35.0%
!! TTTGTTAAGGGTGCTAGTTA+TGG - chr8.1:71987180-71987199 MS.gene019627:CDS 35.0%
!!! CGAATTTCGGATGTTTTTTG+TGG - chr8.1:71986622-71986641 MS.gene019627:intron 35.0%
AATTATGGTGCACGTTCTGA+TGG - chr8.1:71985215-71985234 MS.gene019627:CDS 40.0%
ACAATGTGATTGAGCTCAAG+CGG - chr8.1:71985850-71985869 MS.gene019627:CDS 40.0%
ACCATGCCAAAACATTAGAG+TGG - chr8.1:71987101-71987120 MS.gene019627:intron 40.0%
ACCTTTAGTGGCACAAGTAA+TGG - chr8.1:71986727-71986746 MS.gene019627:CDS 40.0%
ATGCCAAAACATTAGAGTGG+AGG - chr8.1:71987104-71987123 MS.gene019627:intron 40.0%
CAATGGTTATGTCCATCACA+TGG - chr8.1:71986396-71986415 MS.gene019627:intron 40.0%
CAGATGGTATCAAAATAGGG+AGG - chr8.1:71986518-71986537 MS.gene019627:intron 40.0%
CATAGCAATGCAATCATCAC+CGG + chr8.1:71986581-71986600 None:intergenic 40.0%
GGAAGTCTTGGTAAGAATGA+AGG - chr8.1:71986667-71986686 MS.gene019627:intron 40.0%
TCAAGTGTGAACATTGGAAC+CGG - chr8.1:71986559-71986578 MS.gene019627:intron 40.0%
TGATTGCATTGCTATGCTCT+CGG - chr8.1:71986585-71986604 MS.gene019627:intron 40.0%
! AACAAATTGACTGTTGGTGG+AGG - chr8.1:71985572-71985591 MS.gene019627:CDS 40.0%
! AAGTTCATCCAAGCATTGCA+TGG + chr8.1:71985473-71985492 None:intergenic 40.0%
! ACCATTACTTGTGCCACTAA+AGG + chr8.1:71986731-71986750 None:intergenic 40.0%
! AGTCTTTTACTACGACCAGA+AGG + chr8.1:71987144-71987163 None:intergenic 40.0%
! CAACTGTTTTCATCCCAGAA+GGG - chr8.1:71985414-71985433 MS.gene019627:intron 40.0%
! GCTCATTTTGTTGGGAATTG+AGG - chr8.1:71985904-71985923 MS.gene019627:CDS 40.0%
!! CATGTGATGGACATAACCAT+TGG + chr8.1:71986398-71986417 None:intergenic 40.0%
!! GATATTAATTTGCGGCCTTC+TGG - chr8.1:71987126-71987145 MS.gene019627:CDS 40.0%
!! GTACATACCTTTTTGGTGCA+CGG + chr8.1:71986883-71986902 None:intergenic 40.0%
!!! TCCACTCTAATGTTTTGGCA+TGG + chr8.1:71987105-71987124 None:intergenic 40.0%
AATGATAGCCCCAACACAGA+TGG - chr8.1:71986502-71986521 MS.gene019627:intron 45.0%
AGTGATGCATGCAGATGGAA+TGG - chr8.1:71985386-71985405 MS.gene019627:intron 45.0%
ATCTGTGTTGGGGCTATCAT+TGG + chr8.1:71986503-71986522 None:intergenic 45.0%
ATGGTCAAGGATCTTCTGCT+TGG - chr8.1:71985606-71985625 MS.gene019627:CDS 45.0%
CCTGGTCATGGAATTAGTGT+CGG - chr8.1:71986646-71986665 MS.gene019627:intron 45.0%
GATTGCATTGCTATGCTCTC+GGG - chr8.1:71986586-71986605 MS.gene019627:intron 45.0%
TCTGTGTTGGGGCTATCATT+GGG + chr8.1:71986502-71986521 None:intergenic 45.0%
TGGCAATACCGACAATAGAG+TGG - chr8.1:71985235-71985254 MS.gene019627:CDS 45.0%
TTAGTGGTCCATGCAATGCT+TGG - chr8.1:71985462-71985481 MS.gene019627:intron 45.0%
! AAATTGACTGTTGGTGGAGG+TGG - chr8.1:71985575-71985594 MS.gene019627:CDS 45.0%
! GCAACTGTTTTCATCCCAGA+AGG - chr8.1:71985413-71985432 MS.gene019627:intron 45.0%
! GGAATTAGTGTCGGAAGTCT+TGG - chr8.1:71986655-71986674 MS.gene019627:intron 45.0%
! TGCAAGAAGGGGGATTTTGT+TGG + chr8.1:71987206-71987225 None:intergenic 45.0%
!!! GTTTTTTGTGGTCCTGGTCA+TGG - chr8.1:71986634-71986653 MS.gene019627:intron 45.0%
!!! TCGGATGTTTTTTGTGGTCC+TGG - chr8.1:71986628-71986647 MS.gene019627:intron 45.0%
!!! ATGTATTTTTTTTTAAATAA+TGG + chr8.1:71986262-71986281 None:intergenic 5.0%
!!! ATTTTGTTATTAAAAATTAA+GGG + chr8.1:71985771-71985790 None:intergenic 5.0%
!!! TATTTTGTTATTAAAAATTA+AGG + chr8.1:71985772-71985791 None:intergenic 5.0%
!!! TTAAAATTTTATATATTTTG+TGG - chr8.1:71986336-71986355 MS.gene019627:intron 5.0%
CATGGAGTGATGCATGCAGA+TGG - chr8.1:71985381-71985400 MS.gene019627:intron 50.0%
CCGACACTAATTCCATGACC+AGG + chr8.1:71986649-71986668 None:intergenic 50.0%
CTGTGTTGGGGCTATCATTG+GGG + chr8.1:71986501-71986520 None:intergenic 50.0%
TGTGTTGGGGCTATCATTGG+GGG + chr8.1:71986500-71986519 None:intergenic 50.0%
TTGCTATGCTCTCGGGTACT+AGG - chr8.1:71986593-71986612 MS.gene019627:intron 50.0%
!! CATACCTTTTTGGTGCACGG+AGG + chr8.1:71986880-71986899 None:intergenic 50.0%
ACGTCCTCCGTGCACCAAAA+AGG - chr8.1:71986873-71986892 MS.gene019627:CDS 55.0%
GGTGGCAGATTGGATGGTCA+AGG - chr8.1:71985593-71985612 MS.gene019627:CDS 55.0%
! TGTTGGTGGAGGTGGCAGAT+TGG - chr8.1:71985583-71985602 MS.gene019627:CDS 55.0%
!! TTTTTGGTGCACGGAGGACG+TGG + chr8.1:71986874-71986893 None:intergenic 55.0%
GGTGGAGGTGGCAGATTGGA+TGG - chr8.1:71985587-71985606 MS.gene019627:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 71985131 71987233 71985131 ID=MS.gene019627
chr8.1 mRNA 71985131 71987233 71985131 ID=MS.gene019627.t1;Parent=MS.gene019627
chr8.1 exon 71987108 71987233 71987108 ID=MS.gene019627.t1.exon1;Parent=MS.gene019627.t1
chr8.1 CDS 71987108 71987233 71987108 ID=cds.MS.gene019627.t1;Parent=MS.gene019627.t1
chr8.1 exon 71986688 71986996 71986688 ID=MS.gene019627.t1.exon2;Parent=MS.gene019627.t1
chr8.1 CDS 71986688 71986996 71986688 ID=cds.MS.gene019627.t1;Parent=MS.gene019627.t1
chr8.1 exon 71985470 71985991 71985470 ID=MS.gene019627.t1.exon3;Parent=MS.gene019627.t1
chr8.1 CDS 71985470 71985991 71985470 ID=cds.MS.gene019627.t1;Parent=MS.gene019627.t1
chr8.1 exon 71985131 71985361 71985131 ID=MS.gene019627.t1.exon4;Parent=MS.gene019627.t1
chr8.1 CDS 71985131 71985361 71985131 ID=cds.MS.gene019627.t1;Parent=MS.gene019627.t1
Gene Sequence

>MS.gene019627

ATGGGTGCAAAATTTTTGTTTGTGTTGAGCTTACTAGTTTGTATTGCAGAAGCACGCCGTCAATACAAGCTTTTCAATGTGAAGAATTATGGTGCACGTTCTGATGGCAATACCGACAATAGAGTGGTAATTTTCCATGTTCATTCATTCGCATTTTTCTTTTAATTTCTTTTATTTTCTTAAAATTGCAATTTTGATTTTGATGAGCTAACTATAGTTTGTTATTTTCTTATGAAGGCATTTTTAAAAGCATGGAGTGATGCATGCAGATGGAATGGAAAAGCAACTGTTTTCATCCCAGAAGGGACATATATACTTAAGGAAGTTGTATTTAGTGGTCCATGCAATGCTTGGATGAACTTTAAAATTGAAGGACTATTGAAAGCTCCAAGTGATCCATATTCATTTAAGACTGATAATTGGATAAATTTTAGATATGTTAACAAATTGACTGTTGGTGGAGGTGGCAGATTGGATGGTCAAGGATCTTCTGCTTGGAAAATTAATGATTGCAAAAAGAATTCAAATTGTCGCTCTCTTCCTACTGTAAGTTCATTTTATATATACAAATCAATATTATCACTAATGTATACCCTATGAAATTGATGTGGCAGATATATTTTTAATTTTTTTTTATCCCTTAATTTTTAATAACAAAATAACTTATCGTTTTATTCAAAATGAGAGGAAAAAAAATATAAATGGTAAAATATACAATAACAATGTGATTGAGCTCAAGCGGCTAATGAGTATCTGAATTTCAGATTACTAAAGCTCATTTTGTTGGGAATTGAGGGTTAACAAAAAAAAAACGATAAGGTAGAGTATGGAAAAAGTAGAGATTGGACCGAAATACAAACACTCTATCTCTTAATGAAAGTATGTTTCATTATTGTCATGTTTAATTAGTTTCTAGGATCACTTTGCCTCACCATTAAAATCAAAATCATGATAAAATCGAAATTGAAATAAATCATCAAACATTGGTATAGAAGTAAAACAACCTTAATGCTTGTTCTTTTGACCACTATTTAGTTAAGAAATTAGAAATGTAGAGTTTTTAATAATTATTCTAATATACAAAAGGTTACATTTTTTAAAACTCTACTTTTTTATACTTGTAAATTACCATTATTTAAAAAAAAATACATTTTTTTGATACTATAATTATTTTAGCAAGATAATGTTCGTTTCAGATGTGTTAATTAAAATTTTATATATTTTGTGGATATGTTTGGACAGACAATGAAATTTGACTTCATCGCCAATGGTTATGTCCATCACATGGAATCTATCAACAGCAAACAAAGTCATTTCGTATTATTTGGGTGTGAAAACATGACACTTAGAAGACTGAAAATAACAGCCCCCAATGATAGCCCCAACACAGATGGTATCAAAATAGGGAGGTCAAATGGAATAAATATTTCAAGTGTGAACATTGGAACCGGTGATGATTGCATTGCTATGCTCTCGGGTACTAGGAATGTTCGAATTTCGGATGTTTTTTGTGGTCCTGGTCATGGAATTAGTGTCGGAAGTCTTGGTAAGAATGAAGGTGAAGAAGATATTGATGACATTGTTGTTAAGAATTGTACCTTTAGTGGCACAAGTAATGGTGTTAGAATCAAATCGTGGGAGTCTCAATTGAAGAAAACTTTGGTTGTTTCGAATTTTGTGTATGAAGATATTGTGATGGATAATGTTCAATATCCCATTGTCATTGATCAAGATTATTGTCCACGTCCTCCGTGCACCAAAAAGGTATGTACATGTATTCTACGTTCGAATATTATTCTTTTATACCCAGCTTAGTCTTTTATAAGTTTGAGTTCTCTATAGACAATAATTTCTGACAATAATTTTGTGTAGGGGGTTTCAAGTGTACAAATAAGCAATGTGGCTTACAAAAATATTAGAGGAAGTGGAAATACAAAAGTTGCAGCAAGCTTCGAATGCAGCTCAAATAAACCATGCCAAAACATTAGAGTGGAGGATATTAATTTGCGGCCTTCTGGTCGTAGTAAAAGACTAAACAATGTGTGTTTATTTGTTAAGGGTGCTAGTTATGGCCAACAAAATCCCCCTTCTTGCATAAAATAG
Protein sequence

>MS.gene019627.t1

MGAKFLFVLSLLVCIAEARRQYKLFNVKNYGARSDGNTDNRVAFLKAWSDACRWNGKATVFIPEGTYILKEVVFSGPCNAWMNFKIEGLLKAPSDPYSFKTDNWINFRYVNKLTVGGGGRLDGQGSSAWKINDCKKNSNCRSLPTTMKFDFIANGYVHHMESINSKQSHFVLFGCENMTLRRLKITAPNDSPNTDGIKIGRSNGINISSVNIGTGDDCIAMLSGTRNVRISDVFCGPGHGISVGSLGKNEGEEDIDDIVVKNCTFSGTSNGVRIKSWESQLKKTLVVSNFVYEDIVMDNVQYPIVIDQDYCPRPPCTKKGVSSVQISNVAYKNIRGSGNTKVAASFECSSNKPCQNIRVEDINLRPSGRSKRLNNVCLFVKGASYGQQNPPSCIK