AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63570.t1 MTR_5g063430 88.278 273 27 1 2 274 42 309 2.24e-180 502
MS.gene63570.t1 MTR_8g030930 70.423 355 103 1 3 355 45 399 3.62e-178 500
MS.gene63570.t1 MTR_3g438050 60.623 353 138 1 2 354 44 395 2.74e-157 447
MS.gene63570.t1 MTR_1g088160 40.395 354 201 6 7 354 57 406 1.77e-86 267
MS.gene63570.t1 MTR_2g058840 37.690 329 193 4 3 324 41 364 4.54e-85 263
MS.gene63570.t1 MTR_2g058640 37.989 358 208 7 3 353 41 391 1.13e-84 262
MS.gene63570.t1 MTR_2g058670 37.805 328 196 4 3 324 41 366 1.20e-84 263
MS.gene63570.t1 MTR_2g435640 37.815 357 213 6 2 353 70 422 1.44e-82 257
MS.gene63570.t1 MTR_2g049400 38.592 355 208 7 2 354 45 391 1.71e-77 243
MS.gene63570.t1 MTR_5g097860 40.268 298 174 3 3 297 41 337 8.44e-77 240
MS.gene63570.t1 MTR_7g091850 40.122 329 186 7 2 324 93 416 1.84e-76 242
MS.gene63570.t1 MTR_1g052315 39.548 354 203 7 2 350 81 428 6.43e-76 241
MS.gene63570.t1 MTR_2g103410 37.326 359 214 6 2 354 76 429 2.33e-75 239
MS.gene63570.t1 MTR_2g060350 38.279 337 196 6 2 331 90 421 2.73e-74 236
MS.gene63570.t1 MTR_4g083660 37.815 357 210 7 2 354 26 374 5.79e-74 234
MS.gene63570.t1 MTR_4g120320 36.034 358 218 6 2 353 69 421 9.81e-74 234
MS.gene63570.t1 MTR_6g028080 38.068 352 196 9 2 343 47 386 1.02e-70 226
MS.gene63570.t1 MTR_8g065030 34.174 357 222 7 2 352 82 431 9.52e-70 225
MS.gene63570.t1 MTR_7g091840 38.870 301 173 6 2 295 82 378 1.55e-69 223
MS.gene63570.t1 MTR_8g009810 36.901 355 212 8 3 353 53 399 1.14e-66 216
MS.gene63570.t1 MTR_3g030540 38.226 327 193 5 2 324 87 408 4.35e-66 215
MS.gene63570.t1 MTR_6g005630 36.890 328 191 11 2 324 62 378 8.67e-66 214
MS.gene63570.t1 MTR_2g100040 35.494 324 161 4 2 323 40 317 4.91e-65 210
MS.gene63570.t1 MTR_3g084380 38.066 331 185 8 3 324 39 358 7.00e-64 208
MS.gene63570.t1 MTR_2g029170 37.168 339 205 6 18 354 29 361 1.21e-63 206
MS.gene63570.t1 MTR_2g103650 36.667 330 194 8 3 322 46 370 3.20e-63 207
MS.gene63570.t1 MTR_4g084050 37.202 336 186 9 3 324 41 365 8.66e-62 203
MS.gene63570.t1 MTR_3g084360 35.714 350 201 8 3 338 42 381 1.90e-61 202
MS.gene63570.t1 MTR_4g094715 34.125 337 204 7 3 326 83 414 7.94e-61 202
MS.gene63570.t1 MTR_3g054320 36.494 348 198 9 3 342 48 380 1.96e-60 199
MS.gene63570.t1 MTR_5g043350 37.580 314 179 8 3 308 47 351 6.15e-59 196
MS.gene63570.t1 MTR_8g010110 34.000 350 206 8 1 344 47 377 1.97e-58 194
MS.gene63570.t1 MTR_8g010080 33.043 345 213 5 2 344 48 376 1.33e-57 192
MS.gene63570.t1 MTR_8g008765 34.839 310 184 6 2 308 48 342 1.02e-56 188
MS.gene63570.t1 MTR_1g086390 33.138 341 208 8 2 327 98 433 1.98e-56 191
MS.gene63570.t1 MTR_8g010070 34.593 344 205 8 3 342 49 376 4.67e-56 188
MS.gene63570.t1 MTR_7g110050 34.118 340 205 9 2 327 84 418 1.17e-55 189
MS.gene63570.t1 MTR_7g113910 35.987 314 163 7 45 354 74 353 4.23e-55 185
MS.gene63570.t1 MTR_8g010090 32.615 325 196 7 1 320 47 353 1.29e-54 184
MS.gene63570.t1 MTR_0157s0060 37.160 331 186 8 2 324 15 331 3.26e-54 182
MS.gene63570.t1 MTR_4g034020 37.160 331 186 8 2 324 15 331 3.26e-54 182
MS.gene63570.t1 MTR_2g103410 38.911 257 150 4 2 252 76 331 8.39e-54 180
MS.gene63570.t1 MTR_5g033680 31.579 361 228 8 2 350 76 429 1.84e-53 183
MS.gene63570.t1 MTR_5g034090 31.268 355 209 8 2 326 138 487 3.10e-53 184
MS.gene63570.t1 MTR_2g032710 32.911 316 194 7 24 326 106 416 4.27e-51 177
MS.gene63570.t1 MTR_6g005630 34.451 328 176 14 2 324 62 355 1.79e-50 173
MS.gene63570.t1 MTR_8g010040 31.014 345 218 7 3 344 50 377 4.90e-50 172
MS.gene63570.t1 MTR_8g065010 30.287 383 228 10 2 351 124 500 2.18e-49 173
MS.gene63570.t1 MTR_1g013730 32.317 328 195 9 2 324 44 349 2.37e-48 167
MS.gene63570.t1 MTR_1g013740 30.488 328 201 7 2 324 52 357 2.85e-47 165
MS.gene63570.t1 MTR_7g035415 34.066 273 165 6 3 270 58 320 4.99e-47 162
MS.gene63570.t1 MTR_8g010050 38.494 239 136 4 2 236 48 279 5.02e-46 160
MS.gene63570.t1 MTR_1g012390 31.098 328 213 8 2 324 45 364 2.36e-44 157
MS.gene63570.t1 MTR_8g008700 33.333 267 161 5 44 308 14 265 3.09e-43 151
MS.gene63570.t1 MTR_5g034090 32.130 277 158 5 2 248 138 414 4.20e-41 149
MS.gene63570.t1 MTR_8g077320 31.802 283 149 6 2 270 50 302 5.66e-38 139
MS.gene63570.t1 MTR_8g031180 35.047 214 128 3 124 332 73 280 7.08e-35 129
MS.gene63570.t1 MTR_7g072360 49.524 105 31 1 132 236 46 128 4.23e-25 99.0
MS.gene63570.t1 MTR_7g072370 67.213 61 20 0 181 241 1 61 1.17e-20 84.7
MS.gene63570.t1 MTR_4g124980 24.503 302 201 8 66 353 176 464 4.61e-13 70.5
MS.gene63570.t1 MTR_2g042740 23.453 307 198 9 66 353 158 446 8.44e-12 66.6
MS.gene63570.t1 MTR_1g100050 24.000 300 173 8 73 343 157 430 1.35e-11 65.9
MS.gene63570.t1 MTR_1g100060 24.422 303 174 10 70 343 197 473 9.86e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63570.t1 AT1G78400 47.025 353 186 1 3 355 53 404 1.33e-116 344
MS.gene63570.t1 AT2G33160 47.740 354 181 3 3 353 50 402 4.16e-111 338
MS.gene63570.t1 AT1G43080 45.225 356 190 4 3 354 49 403 4.51e-107 320
MS.gene63570.t1 AT1G17150 44.602 352 194 1 3 354 51 401 8.39e-107 319
MS.gene63570.t1 AT2G15450 45.225 356 190 4 3 354 49 403 1.55e-106 318
MS.gene63570.t1 AT4G13760 45.000 360 185 5 3 354 49 403 1.06e-105 316
MS.gene63570.t1 AT1G43090 46.132 349 183 4 3 347 49 396 1.69e-105 317
MS.gene63570.t1 AT2G15460 45.170 352 188 4 3 350 49 399 1.02e-104 313
MS.gene63570.t1 AT2G15470 44.663 356 192 4 3 354 49 403 1.25e-103 311
MS.gene63570.t1 AT1G43100 45.559 349 185 4 3 347 49 396 5.58e-103 310
MS.gene63570.t1 AT5G48140 41.595 351 202 2 3 353 39 386 2.84e-101 304
MS.gene63570.t1 AT2G40310 43.889 360 189 6 3 354 49 403 4.87e-101 304
MS.gene63570.t1 AT2G26620 44.350 354 194 3 3 354 49 401 8.67e-101 303
MS.gene63570.t1 AT3G07820 41.643 353 202 3 2 353 37 386 2.69e-100 302
MS.gene63570.t1 AT3G07840 40.793 353 205 3 2 353 38 387 7.87e-98 296
MS.gene63570.t1 AT4G18180 41.144 367 200 5 2 353 56 421 1.30e-96 293
MS.gene63570.t1 AT3G07830 39.943 353 208 3 2 353 38 387 1.68e-95 290
MS.gene63570.t1 AT3G14040 39.706 340 201 3 18 355 102 439 5.95e-88 272
MS.gene63570.t1 AT3G07850 39.412 340 202 3 18 355 101 438 9.71e-88 271
MS.gene63570.t1 AT1G80170 37.883 359 213 5 2 354 70 424 5.42e-86 267
MS.gene63570.t1 AT2G43890 38.936 357 204 9 2 354 46 392 4.67e-81 253
MS.gene63570.t1 AT3G07970 39.548 354 205 6 2 350 87 436 5.12e-81 254
MS.gene63570.t1 AT2G41850 41.337 329 182 7 2 324 85 408 1.27e-77 245
MS.gene63570.t1 AT3G57510 38.953 344 196 8 2 336 85 423 1.82e-76 242
MS.gene63570.t1 AT1G05650 38.580 324 191 5 3 324 46 363 1.20e-75 239
MS.gene63570.t1 AT1G02790 36.438 365 212 6 1 355 68 422 1.29e-74 237
MS.gene63570.t1 AT1G05660 38.580 324 191 5 3 324 46 363 2.72e-73 233
MS.gene63570.t1 AT3G59850 38.655 357 207 8 2 354 40 388 3.23e-72 229
MS.gene63570.t1 AT2G43880 37.963 324 195 4 2 324 48 366 5.22e-72 229
MS.gene63570.t1 AT2G43870 37.677 353 207 9 2 350 38 381 1.20e-71 228
MS.gene63570.t1 AT3G59850 38.655 357 207 8 2 354 84 432 1.71e-71 229
MS.gene63570.t1 AT4G35670 37.464 347 200 8 2 342 41 376 1.18e-67 218
MS.gene63570.t1 AT1G70500 33.333 357 225 8 2 352 84 433 2.64e-67 219
MS.gene63570.t1 AT2G43860 39.024 328 188 8 2 324 50 370 2.71e-67 218
MS.gene63570.t1 AT5G17200 37.097 372 197 9 2 348 56 415 5.29e-66 214
MS.gene63570.t1 AT1G23460 32.773 357 227 7 2 352 60 409 1.25e-65 214
MS.gene63570.t1 AT2G43880 40.659 273 156 4 2 273 48 315 1.34e-65 211
MS.gene63570.t1 AT1G23460 32.773 357 227 7 2 352 84 433 1.98e-65 214
MS.gene63570.t1 AT1G65570 36.592 358 214 9 2 354 48 397 6.17e-65 211
MS.gene63570.t1 AT5G14650 36.842 361 212 12 2 355 68 419 3.82e-64 210
MS.gene63570.t1 AT3G15720 35.043 351 206 9 2 343 40 377 7.96e-61 202
MS.gene63570.t1 AT3G15720 35.043 351 206 9 2 343 41 378 9.06e-61 202
MS.gene63570.t1 AT5G44840 33.889 360 222 8 2 355 41 390 1.22e-60 200
MS.gene63570.t1 AT1G80170 39.918 243 139 4 2 238 70 311 4.85e-60 196
MS.gene63570.t1 AT1G48100 33.426 359 217 10 3 348 98 447 5.30e-60 200
MS.gene63570.t1 AT5G27530 35.632 348 195 10 3 342 71 397 2.06e-58 196
MS.gene63570.t1 AT4G32380 34.006 347 212 6 2 340 44 381 4.76e-58 195
MS.gene63570.t1 AT4G32375 36.749 283 169 4 59 340 8 281 8.21e-58 193
MS.gene63570.t1 AT1G56710 34.604 341 203 8 2 327 65 400 1.02e-57 193
MS.gene63570.t1 AT3G26610 35.030 334 203 7 2 327 84 411 1.14e-57 194
MS.gene63570.t1 AT3G15720 34.758 351 204 10 2 343 40 374 1.24e-57 194
MS.gene63570.t1 AT1G60590 32.402 358 204 8 2 326 140 492 6.03e-57 194
MS.gene63570.t1 AT5G44830 33.626 342 202 7 19 355 10 331 1.05e-56 188
MS.gene63570.t1 AT1G10640 30.709 381 226 8 2 352 136 508 2.28e-55 189
MS.gene63570.t1 AT1G02460 33.062 369 224 10 2 352 106 469 5.49e-55 187
MS.gene63570.t1 AT4G32370 38.869 283 153 8 2 273 52 325 5.63e-55 184
MS.gene63570.t1 AT4G35670 36.559 279 166 4 64 342 2 269 6.19e-55 182
MS.gene63570.t1 AT3G15720 34.969 326 191 8 26 343 2 314 6.42e-55 185
MS.gene63570.t1 AT1G80140 33.236 343 211 8 19 353 3 335 4.40e-53 179
MS.gene63570.t1 AT4G01890 32.754 345 205 11 3 327 83 420 1.11e-52 181
MS.gene63570.t1 AT5G14650 40.496 242 135 7 2 238 68 305 1.21e-48 166
MS.gene63570.t1 AT5G39910 34.459 296 163 9 2 288 53 326 9.07e-47 163
MS.gene63570.t1 AT4G32380 29.855 345 188 5 2 340 44 340 1.26e-44 158
MS.gene63570.t1 AT4G23820 25.995 377 218 14 16 353 74 428 4.22e-15 76.6
MS.gene63570.t1 AT4G33440 25.091 275 167 8 71 324 177 433 1.56e-11 65.9
MS.gene63570.t1 AT3G48950 21.284 296 205 8 73 353 153 435 5.08e-11 64.3

Find 71 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAGCAAGCAGATTCATTT+TGG 0.148312 3.2:-25549110 MS.gene63570:CDS
TTATCACTGGCAAATGAATT+TGG 0.172143 3.2:+25550390 None:intergenic
TTTAAGCAATGTTCGAATTA+TGG 0.186282 3.2:-25548928 MS.gene63570:CDS
ATTCATTTGCCAGTGATAAT+TGG 0.206426 3.2:-25550386 MS.gene63570:CDS
TATTCTCCATTGTGATGTTT+TGG 0.274484 3.2:+25548435 None:intergenic
AATACAGATGGCATCAAAAT+AGG 0.309565 3.2:-25549023 MS.gene63570:CDS
TTTGTAAGTGGAGCTGTTTA+TGG 0.321171 3.2:-25548362 MS.gene63570:CDS
TGCTTCTAATTTCATATATG+AGG 0.326286 3.2:-25548742 MS.gene63570:CDS
GTAGGAAGAGCACGACAATT+AGG 0.334277 3.2:+25550264 None:intergenic
CAAACATGTTAACAAGCTAA+TGG 0.342351 3.2:-25550355 MS.gene63570:CDS
TCTGTATTGGGGCTATCCCC+GGG 0.345274 3.2:+25549038 None:intergenic
ATCTGTATTGGGGCTATCCC+CGG 0.346109 3.2:+25549037 None:intergenic
TTGTGGCCTTCTAGTACTAA+TGG 0.362144 3.2:-25548407 MS.gene63570:CDS
TTGTAAGTGGAGCTGTTTAT+GGG 0.383566 3.2:-25548361 MS.gene63570:CDS
GAATTCTCTATGCCGCAAAA+AGG 0.387633 3.2:-25548664 MS.gene63570:intron
TTAAGCTTATACGTCCCATT+TGG 0.393451 3.2:+25550489 None:intergenic
GCAAATGAATTTGGATCAAT+TGG 0.406157 3.2:+25550399 None:intergenic
AGGTGGATCATTAGATGGTC+AGG 0.414528 3.2:-25550325 MS.gene63570:CDS
AAGCTAATGGTTGGTGGAGG+TGG 0.415356 3.2:-25550342 MS.gene63570:CDS
GTTAAGAATTGCACATTGAA+TGG 0.429601 3.2:-25548822 MS.gene63570:CDS
TAGGAAGAGCACGACAATTA+GGG 0.429828 3.2:+25550265 None:intergenic
TACAAATACATTAGAGGGAG+TGG 0.445217 3.2:-25548506 MS.gene63570:CDS
TGACTGCATTTCTATGCTCT+CGG 0.447737 3.2:-25548952 MS.gene63570:CDS
GACTGCATTTCTATGCTCTC+GGG 0.449774 3.2:-25548951 MS.gene63570:CDS
AGCCTTCCATTAGTACTAGA+AGG 0.455737 3.2:+25548401 None:intergenic
GTGGCTTACAAATACATTAG+AGG 0.462691 3.2:-25548512 MS.gene63570:CDS
GGTGGAGGTGGATCATTAGA+TGG 0.466751 3.2:-25550330 MS.gene63570:CDS
GAAATTGATCCAATTATCAC+TGG 0.470074 3.2:+25550377 None:intergenic
AATGAGTGTTCATTTGTAAG+TGG 0.471217 3.2:-25548374 MS.gene63570:CDS
GGAATAAGCATCGGAAGTCT+TGG 0.479126 3.2:-25548882 MS.gene63570:CDS
GTGTATTGTGGTCCTGGTCA+TGG 0.489493 3.2:-25548903 MS.gene63570:CDS
ATGGATGTGTATTGTGGTCC+TGG 0.496657 3.2:-25548909 MS.gene63570:CDS
ATGGTCAGGGAGCTTATGCT+TGG 0.498086 3.2:-25550311 MS.gene63570:CDS
ACAAGTGTTGACATTAGAAC+GGG 0.498201 3.2:-25548978 MS.gene63570:CDS
TAGTGTTGATCAATTACAAT+GGG 0.514314 3.2:+25548693 None:intergenic
CATGGAGGGATGCATGCAAA+TGG 0.514976 3.2:-25550536 MS.gene63570:CDS
AAATTTGATTACATTACCAA+TGG 0.518660 3.2:-25549158 MS.gene63570:CDS
AATTTGATTACATTACCAAT+GGG 0.519096 3.2:-25549157 MS.gene63570:CDS
TATCGAAGAATTACATGGAC+CGG 0.522528 3.2:+25550452 None:intergenic
AAATTTATCGAAGAATTACA+TGG 0.537006 3.2:+25550447 None:intergenic
CAATGGAGAATATCGATTTG+TGG 0.546964 3.2:-25548424 MS.gene63570:CDS
ACATTGAATGGTACGAGTAA+TGG 0.549418 3.2:-25548810 MS.gene63570:CDS
CCGATGCTTATTCCATGACC+AGG 0.552380 3.2:+25548891 None:intergenic
GCAATTCTTAACAATTATGT+CGG 0.553416 3.2:+25548833 None:intergenic
CGAATTATGGATGTGTATTG+TGG 0.562563 3.2:-25548915 MS.gene63570:CDS
CTTAAAGATGTCAACTTCAC+CGG 0.564526 3.2:-25550471 MS.gene63570:CDS
CTGTATTGGGGCTATCCCCG+GGG 0.566674 3.2:+25549039 None:intergenic
CATCAAAATAGGAAAGTCAA+GGG 0.567415 3.2:-25549012 MS.gene63570:CDS
CATATGATGTATATACCCAT+TGG 0.567415 3.2:+25549142 None:intergenic
CATGTTAACAAGCTAATGGT+TGG 0.569252 3.2:-25550351 MS.gene63570:CDS
CATATATGAGGATATTGTGA+TGG 0.570079 3.2:-25548730 MS.gene63570:CDS
TAATTGTCGTGCTCTTCCTA+CGG 0.589306 3.2:-25550262 MS.gene63570:intron
GTTAACAAGCTAATGGTTGG+TGG 0.591735 3.2:-25550348 MS.gene63570:CDS
TGGCTTACAAATACATTAGA+GGG 0.591791 3.2:-25548511 MS.gene63570:CDS
AAACTACAAATAAAAGCCCC+CGG 0.591878 3.2:-25549056 MS.gene63570:CDS
CACAAGTGTTGACATTAGAA+CGG 0.601694 3.2:-25548979 MS.gene63570:CDS
GCATCAAAATAGGAAAGTCA+AGG 0.603398 3.2:-25549013 MS.gene63570:CDS
GGATATTGTGATGGAAAATG+TGG 0.613627 3.2:-25548721 MS.gene63570:CDS
GGGGATAGCCCCAATACAGA+TGG 0.615320 3.2:-25549035 MS.gene63570:CDS
GGTGGATCATTAGATGGTCA+GGG 0.618305 3.2:-25550324 MS.gene63570:CDS
CCTGGTCATGGAATAAGCAT+CGG 0.624229 3.2:-25548891 MS.gene63570:CDS
GGCCTTCTAGTACTAATGGA+AGG 0.625650 3.2:-25548403 MS.gene63570:CDS
ATAGTGTTGATCAATTACAA+TGG 0.636794 3.2:+25548692 None:intergenic
TATGAAATTAGAAGCAACCA+CGG 0.666620 3.2:+25548749 None:intergenic
TGTATTGGGGCTATCCCCGG+GGG 0.669196 3.2:+25549040 None:intergenic
AGGGATGCATGCAAATGGAG+TGG 0.669395 3.2:-25550531 MS.gene63570:CDS
AACAAGCTAATGGTTGGTGG+AGG 0.670101 3.2:-25550345 MS.gene63570:CDS
ACCATGCCAAAACATCACAA+TGG 0.672824 3.2:-25548441 MS.gene63570:CDS
ATCAAAATAGGAAAGTCAAG+GGG 0.696894 3.2:-25549011 MS.gene63570:CDS
AAGCGTACAAATACGCAACG+TGG 0.715703 3.2:-25548531 MS.gene63570:CDS
GTCACAACTGAACAAAACCG+TGG 0.734259 3.2:-25548766 MS.gene63570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAGTTATATATAATAC+TGG - chr3.2:25549032-25549051 MS.gene63570:CDS 10.0%
!! ATATAGTAAAAAAAAAAGAA+TGG + chr3.2:25548676-25548695 None:intergenic 10.0%
!!! ATTAACGTTTAATATTTTTT+TGG - chr3.2:25549688-25549707 MS.gene63570:intron 10.0%
!! ATAAAACTATCATCAATATA+TGG + chr3.2:25550305-25550324 None:intergenic 15.0%
!! ATTTGAGTAAGTATAATTAA+GGG - chr3.2:25549063-25549082 MS.gene63570:CDS 15.0%
!! TATTTGAGTAAGTATAATTA+AGG - chr3.2:25549062-25549081 MS.gene63570:CDS 15.0%
!! TTACAATTTGATTCAAAATA+CGG - chr3.2:25548820-25548839 MS.gene63570:CDS 15.0%
!! AAATTTATCGAAGAATTACA+TGG + chr3.2:25548442-25548461 None:intergenic 20.0%
!! AAATTTGATTACATTACCAA+TGG - chr3.2:25549728-25549747 MS.gene63570:intron 20.0%
!! AATTTGATTACATTACCAAT+GGG - chr3.2:25549729-25549748 MS.gene63570:intron 20.0%
!! CTTTAGCTATATCTATATTT+CGG + chr3.2:25549617-25549636 None:intergenic 20.0%
!! TACAATTTGATTCAAAATAC+GGG - chr3.2:25548821-25548840 MS.gene63570:CDS 20.0%
!!! ACCTGCTTTTAATAATTATA+GGG - chr3.2:25549326-25549345 MS.gene63570:intron 20.0%
!!! TACCTGCTTTTAATAATTAT+AGG - chr3.2:25549325-25549344 MS.gene63570:intron 20.0%
!!! TACGATTTTCGTATAATTAA+GGG - chr3.2:25549165-25549184 MS.gene63570:CDS 20.0%
!!! TTACGATTTTCGTATAATTA+AGG - chr3.2:25549164-25549183 MS.gene63570:CDS 20.0%
! ACCCTATAATTATTAAAAGC+AGG + chr3.2:25549330-25549349 None:intergenic 25.0%
! ATGAAGCATGTGAAAAAAAT+AGG - chr3.2:25549584-25549603 MS.gene63570:intron 25.0%
! CAAATTTATTAACTTACCGT+AGG + chr3.2:25548643-25548662 None:intergenic 25.0%
! GCAATTCTTAACAATTATGT+CGG + chr3.2:25550056-25550075 None:intergenic 25.0%
! TGAAGCATGTGAAAAAAATA+GGG - chr3.2:25549585-25549604 MS.gene63570:intron 25.0%
! TGCTTCTAATTTCATATATG+AGG - chr3.2:25550144-25550163 MS.gene63570:intron 25.0%
! TTCAACACTTAGATTTAAGA+GGG + chr3.2:25548704-25548723 None:intergenic 25.0%
! TTTAAGCAATGTTCGAATTA+TGG - chr3.2:25549958-25549977 MS.gene63570:intron 25.0%
! TTTCTCTAAATTAGTGAAAG+AGG - chr3.2:25550256-25550275 MS.gene63570:intron 25.0%
!! TCGATAAATTTTAGACTTGA+AGG - chr3.2:25548451-25548470 MS.gene63570:CDS 25.0%
!!! ATAGTGTTGATCAATTACAA+TGG + chr3.2:25550197-25550216 None:intergenic 25.0%
!!! CTACTGTTTTAATTCCAAAT+GGG - chr3.2:25548383-25548402 MS.gene63570:CDS 25.0%
!!! GAACTCTATTTTTAAGGAAT+TGG - chr3.2:25549539-25549558 MS.gene63570:intron 25.0%
!!! GATTTTCTCTTTGAAGTTTA+GGG - chr3.2:25549270-25549289 MS.gene63570:intron 25.0%
!!! TAGTGTTGATCAATTACAAT+GGG + chr3.2:25550196-25550215 None:intergenic 25.0%
!!! TCACATGAACTCTATTTTTA+AGG - chr3.2:25549533-25549552 MS.gene63570:intron 25.0%
!!! TCTACTGTTTTAATTCCAAA+TGG - chr3.2:25548382-25548401 MS.gene63570:CDS 25.0%
AATACAGATGGCATCAAAAT+AGG - chr3.2:25549863-25549882 MS.gene63570:intron 30.0%
AATGAGTGTTCATTTGTAAG+TGG - chr3.2:25550512-25550531 MS.gene63570:CDS 30.0%
ATCAAAATAGGAAAGTCAAG+GGG - chr3.2:25549875-25549894 MS.gene63570:intron 30.0%
ATCTCTCTTAATTTCATGCA+TGG - chr3.2:25548781-25548800 MS.gene63570:CDS 30.0%
ATTCATTTGCCAGTGATAAT+TGG - chr3.2:25548500-25548519 MS.gene63570:CDS 30.0%
CAAACATGTTAACAAGCTAA+TGG - chr3.2:25548531-25548550 MS.gene63570:CDS 30.0%
CATATATGAGGATATTGTGA+TGG - chr3.2:25550156-25550175 MS.gene63570:intron 30.0%
CATATGATGTATATACCCAT+TGG + chr3.2:25549747-25549766 None:intergenic 30.0%
CATCAAAATAGGAAAGTCAA+GGG - chr3.2:25549874-25549893 MS.gene63570:intron 30.0%
CTTCAACACTTAGATTTAAG+AGG + chr3.2:25548705-25548724 None:intergenic 30.0%
GAAATTGATCCAATTATCAC+TGG + chr3.2:25548512-25548531 None:intergenic 30.0%
GAAGCATGTGAAAAAAATAG+GGG - chr3.2:25549586-25549605 MS.gene63570:intron 30.0%
GCAAATGAATTTGGATCAAT+TGG + chr3.2:25548490-25548509 None:intergenic 30.0%
GTTAAGAATTGCACATTGAA+TGG - chr3.2:25550064-25550083 MS.gene63570:intron 30.0%
TATGAAATTAGAAGCAACCA+CGG + chr3.2:25550140-25550159 None:intergenic 30.0%
TGGCTTACAAATACATTAGA+GGG - chr3.2:25550375-25550394 MS.gene63570:CDS 30.0%
TTATCACTGGCAAATGAATT+TGG + chr3.2:25548499-25548518 None:intergenic 30.0%
! ATTAAGTGGATTCCATTCTA+TGG + chr3.2:25548754-25548773 None:intergenic 30.0%
! TATTCTCCATTGTGATGTTT+TGG + chr3.2:25550454-25550473 None:intergenic 30.0%
!! ATTTTGATGCCATCTGTATT+GGG + chr3.2:25549863-25549882 None:intergenic 30.0%
!!! CGATTTTCTCTTTGAAGTTT+AGG - chr3.2:25549269-25549288 MS.gene63570:intron 30.0%
!!! TATTTTGATGCCATCTGTAT+TGG + chr3.2:25549864-25549883 None:intergenic 30.0%
AAACTACAAATAAAAGCCCC+CGG - chr3.2:25549830-25549849 MS.gene63570:intron 35.0%
AATTATAGGGTTGCGATTGT+CGG - chr3.2:25549339-25549358 MS.gene63570:intron 35.0%
ACATTGAATGGTACGAGTAA+TGG - chr3.2:25550076-25550095 MS.gene63570:intron 35.0%
AGCAGGTACTATGATAAAAG+AGG + chr3.2:25549313-25549332 None:intergenic 35.0%
CATGTGAAAAAAATAGGGGT+TGG - chr3.2:25549590-25549609 MS.gene63570:intron 35.0%
CATGTTAACAAGCTAATGGT+TGG - chr3.2:25548535-25548554 MS.gene63570:CDS 35.0%
CGAATTATGGATGTGTATTG+TGG - chr3.2:25549971-25549990 MS.gene63570:intron 35.0%
CTTAAAGATGTCAACTTCAC+CGG - chr3.2:25548415-25548434 MS.gene63570:CDS 35.0%
GCATCAAAATAGGAAAGTCA+AGG - chr3.2:25549873-25549892 MS.gene63570:intron 35.0%
GTGGCTTACAAATACATTAG+AGG - chr3.2:25550374-25550393 MS.gene63570:CDS 35.0%
TAACCCCAAAACAAAAACTG+AGG + chr3.2:25549147-25549166 None:intergenic 35.0%
TACAAATACATTAGAGGGAG+TGG - chr3.2:25550380-25550399 MS.gene63570:CDS 35.0%
TACCTGCCAATAATTGTGTA+GGG - chr3.2:25550326-25550345 MS.gene63570:CDS 35.0%
TATCGAAGAATTACATGGAC+CGG + chr3.2:25548437-25548456 None:intergenic 35.0%
TCCATTCTATGGTAGTAAGT+GGG + chr3.2:25548743-25548762 None:intergenic 35.0%
TTAAGCTTATACGTCCCATT+TGG + chr3.2:25548400-25548419 None:intergenic 35.0%
TTCCATTCTATGGTAGTAAG+TGG + chr3.2:25548744-25548763 None:intergenic 35.0%
TTGTAAGTGGAGCTGTTTAT+GGG - chr3.2:25550525-25550544 MS.gene63570:CDS 35.0%
TTTGTAAGTGGAGCTGTTTA+TGG - chr3.2:25550524-25550543 MS.gene63570:CDS 35.0%
! AACAGCAAGCAGATTCATTT+TGG - chr3.2:25549776-25549795 MS.gene63570:intron 35.0%
! ACAGCAAGCAGATTCATTTT+GGG - chr3.2:25549777-25549796 MS.gene63570:intron 35.0%
! ACCCAAAACAAACCTTTTTG+CGG + chr3.2:25550237-25550256 None:intergenic 35.0%
! GGATATTGTGATGGAAAATG+TGG - chr3.2:25550165-25550184 MS.gene63570:intron 35.0%
! TTTTGATGCCATCTGTATTG+GGG + chr3.2:25549862-25549881 None:intergenic 35.0%
! TTTTTGCGGCATAGAGAATT+CGG + chr3.2:25550223-25550242 None:intergenic 35.0%
!! ACAAGTGTTGACATTAGAAC+GGG - chr3.2:25549908-25549927 MS.gene63570:intron 35.0%
!! CAATGGAGAATATCGATTTG+TGG - chr3.2:25550462-25550481 MS.gene63570:CDS 35.0%
!! CACAAGTGTTGACATTAGAA+CGG - chr3.2:25549907-25549926 MS.gene63570:intron 35.0%
AACCAAATGATGCACTCACT+TGG - chr3.2:25549486-25549505 MS.gene63570:intron 40.0%
AACCAAGTGAGTGCATCATT+TGG + chr3.2:25549491-25549510 None:intergenic 40.0%
AACTACAAATAAAAGCCCCC+GGG - chr3.2:25549831-25549850 MS.gene63570:intron 40.0%
ACCATGCCAAAACATCACAA+TGG - chr3.2:25550445-25550464 MS.gene63570:CDS 40.0%
ACCCACTTACTACCATAGAA+TGG - chr3.2:25548739-25548758 MS.gene63570:CDS 40.0%
ACCTGCCAATAATTGTGTAG+GGG - chr3.2:25550327-25550346 MS.gene63570:CDS 40.0%
AGCCTTCCATTAGTACTAGA+AGG + chr3.2:25550488-25550507 None:intergenic 40.0%
CCAAAACAAAAACTGAGGCA+GGG + chr3.2:25549142-25549161 None:intergenic 40.0%
GAAACCCCCTACACAATTAT+TGG + chr3.2:25550335-25550354 None:intergenic 40.0%
GAATTCTCTATGCCGCAAAA+AGG - chr3.2:25550222-25550241 MS.gene63570:intron 40.0%
GAGAGATAGCTGCAATTAAG+TGG + chr3.2:25548768-25548787 None:intergenic 40.0%
GTACCTGCCAATAATTGTGT+AGG - chr3.2:25550325-25550344 MS.gene63570:CDS 40.0%
GTTAACAAGCTAATGGTTGG+TGG - chr3.2:25548538-25548557 MS.gene63570:CDS 40.0%
TAATTGTCGTGCTCTTCCTA+CGG - chr3.2:25548624-25548643 MS.gene63570:intron 40.0%
TGACTGCATTTCTATGCTCT+CGG - chr3.2:25549934-25549953 MS.gene63570:intron 40.0%
TTGTGGCCTTCTAGTACTAA+TGG - chr3.2:25550479-25550498 MS.gene63570:CDS 40.0%
! ACACGTGTTTTCTCAGTTTG+GGG + chr3.2:25548869-25548888 None:intergenic 40.0%
! CACACGTGTTTTCTCAGTTT+GGG + chr3.2:25548870-25548889 None:intergenic 40.0%
! TAGGAAGAGCACGACAATTA+GGG + chr3.2:25548624-25548643 None:intergenic 40.0%
!! GCCGCAAAAAGGTTTGTTTT+GGG - chr3.2:25550233-25550252 MS.gene63570:intron 40.0%
!! TGCCGCAAAAAGGTTTGTTT+TGG - chr3.2:25550232-25550251 MS.gene63570:intron 40.0%
!!! CCTGCCTCAGTTTTTGTTTT+GGG - chr3.2:25549140-25549159 MS.gene63570:CDS 40.0%
!!! CTGCCTCAGTTTTTGTTTTG+GGG - chr3.2:25549141-25549160 MS.gene63570:CDS 40.0%
!!! TCCATTGTGATGTTTTGGCA+TGG + chr3.2:25550449-25550468 None:intergenic 40.0%
!!! TGAAGGCGTTTTTGAATGCA+TGG - chr3.2:25548332-25548351 MS.gene63570:CDS 40.0%
AAGCGTACAAATACGCAACG+TGG - chr3.2:25550355-25550374 MS.gene63570:CDS 45.0%
ACTACAAATAAAAGCCCCCG+GGG - chr3.2:25549832-25549851 MS.gene63570:intron 45.0%
AGGTGGATCATTAGATGGTC+AGG - chr3.2:25548561-25548580 MS.gene63570:intron 45.0%
ATGGATGTGTATTGTGGTCC+TGG - chr3.2:25549977-25549996 MS.gene63570:intron 45.0%
CCAAACTGAGAAAACACGTG+TGG - chr3.2:25548868-25548887 MS.gene63570:CDS 45.0%
CCCAAAACAAAAACTGAGGC+AGG + chr3.2:25549143-25549162 None:intergenic 45.0%
CCTGCCAATAATTGTGTAGG+GGG - chr3.2:25550328-25550347 MS.gene63570:CDS 45.0%
CCTGGTCATGGAATAAGCAT+CGG - chr3.2:25549995-25550014 MS.gene63570:intron 45.0%
GACTGCATTTCTATGCTCTC+GGG - chr3.2:25549935-25549954 MS.gene63570:intron 45.0%
GGCCTTCTAGTACTAATGGA+AGG - chr3.2:25550483-25550502 MS.gene63570:CDS 45.0%
GGTGGATCATTAGATGGTCA+GGG - chr3.2:25548562-25548581 MS.gene63570:intron 45.0%
GTCACAACTGAACAAAACCG+TGG - chr3.2:25550120-25550139 MS.gene63570:intron 45.0%
! AGGCGTTTTTGAATGCATGG+AGG - chr3.2:25548335-25548354 MS.gene63570:CDS 45.0%
! CACGTGTTTTCTCAGTTTGG+GGG + chr3.2:25548868-25548887 None:intergenic 45.0%
! CCACACGTGTTTTCTCAGTT+TGG + chr3.2:25548871-25548890 None:intergenic 45.0%
! GGCGTTTTTGAATGCATGGA+GGG - chr3.2:25548336-25548355 MS.gene63570:CDS 45.0%
! GTAGGAAGAGCACGACAATT+AGG + chr3.2:25548625-25548644 None:intergenic 45.0%
!! AACAAGCTAATGGTTGGTGG+AGG - chr3.2:25548541-25548560 MS.gene63570:CDS 45.0%
!! CCCTGCCTCAGTTTTTGTTT+TGG - chr3.2:25549139-25549158 MS.gene63570:CDS 45.0%
!! GGAATAAGCATCGGAAGTCT+TGG - chr3.2:25550004-25550023 MS.gene63570:intron 45.0%
AGGGATGCATGCAAATGGAG+TGG - chr3.2:25548355-25548374 MS.gene63570:CDS 50.0%
ATCTGTATTGGGGCTATCCC+CGG + chr3.2:25549852-25549871 None:intergenic 50.0%
ATGGTCAGGGAGCTTATGCT+TGG - chr3.2:25548575-25548594 MS.gene63570:intron 50.0%
CATGGAGGGATGCATGCAAA+TGG - chr3.2:25548350-25548369 MS.gene63570:CDS 50.0%
CCCCCTACACAATTATTGGC+AGG + chr3.2:25550331-25550350 None:intergenic 50.0%
CCGATGCTTATTCCATGACC+AGG + chr3.2:25549998-25550017 None:intergenic 50.0%
GGTGGAGGTGGATCATTAGA+TGG - chr3.2:25548556-25548575 MS.gene63570:intron 50.0%
GTGTATTGTGGTCCTGGTCA+TGG - chr3.2:25549983-25550002 MS.gene63570:intron 50.0%
!! AAGCTAATGGTTGGTGGAGG+TGG - chr3.2:25548544-25548563 MS.gene63570:intron 50.0%
GGGGATAGCCCCAATACAGA+TGG - chr3.2:25549851-25549870 MS.gene63570:intron 55.0%
TCTGTATTGGGGCTATCCCC+GGG + chr3.2:25549851-25549870 None:intergenic 55.0%
CTGTATTGGGGCTATCCCCG+GGG + chr3.2:25549850-25549869 None:intergenic 60.0%
TGTATTGGGGCTATCCCCGG+GGG + chr3.2:25549849-25549868 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 25548331 25550577 25548331 ID=MS.gene63570
chr3.2 mRNA 25548331 25550577 25548331 ID=MS.gene63570.t1;Parent=MS.gene63570
chr3.2 exon 25550263 25550577 25550263 ID=MS.gene63570.t1.exon1;Parent=MS.gene63570.t1
chr3.2 CDS 25550263 25550577 25550263 ID=cds.MS.gene63570.t1;Parent=MS.gene63570.t1
chr3.2 exon 25548665 25549186 25548665 ID=MS.gene63570.t1.exon2;Parent=MS.gene63570.t1
chr3.2 CDS 25548665 25549186 25548665 ID=cds.MS.gene63570.t1;Parent=MS.gene63570.t1
chr3.2 exon 25548331 25548561 25548331 ID=MS.gene63570.t1.exon3;Parent=MS.gene63570.t1
chr3.2 CDS 25548331 25548561 25548331 ID=cds.MS.gene63570.t1;Parent=MS.gene63570.t1
Gene Sequence

>MS.gene63570

ATGAAGGCGTTTTTGAATGCATGGAGGGATGCATGCAAATGGAGTGGAAAATCTACTGTTTTAATTCCAAATGGGACGTATAAGCTTAAAGATGTCAACTTCACCGGTCCATGTAATTCTTCGATAAATTTTAGACTTGAAGGAATGTTGAAAGCACCAATTGATCCAAATTCATTTGCCAGTGATAATTGGATCAATTTCAAACATGTTAACAAGCTAATGGTTGGTGGAGGTGGATCATTAGATGGTCAGGGAGCTTATGCTTGGAAAATGAATGATTGTAAAAAGAACCCTAATTGTCGTGCTCTTCCTACGGTAAGTTAATAAATTTGCACACATTATCCATTCTTTTTTTTTTACTATATATGAACCCTCTTAAATCTAAGTGTTGAAGCATGTTTAATTTGCACCCACTTACTACCATAGAATGGAATCCACTTAATTGCAGCTATCTCTCTTAATTTCATGCATGGAATTCATTAATTATGATTACAATTTGATTCAAAATACGGGTGAATATATTTGAGCACTTTGCCCCCAAACTGAGAAAACACGTGTGGTATATATGTATTGATTCATATCATATATAGAAATATTGTAAATAATTTATTTTAACTATATTTAAATATATGCTAAAATTTTATAATTCAAATATTTATATCTTTATCATATTAAAAAGTTTTTTAGTGACATGTTATATTAAAAAAGTTATATATAATACTGGATAATTTTATTTGAGTAAGTATAATTAAGGGCAATGTAATTTTTTTTTATTATTATAATTATGAATTGAAGTTGAAAGTTTAAACCCTGCCTCAGTTTTTGTTTTGGGGTTACGATTTTCGTATAATTAAGGGCAATGTAATTTTTTTTTATTATTATAATTATGAATTGAAGTTGAAAGTTTAAACCCTGCCTCAGTTTTTGTTTTGGGGTTACGATTTTCTCTTTGAAGTTTAGGGTCTCTTTGTATAGAATGCCTCTTTTATCATAGTACCTGCTTTTAATAATTATAGGGTTGCGATTGTCGGAAATTTACTTTTTTGCTGCTTCAAATTATTTTGTATTTTTTTCAGTTTTTGCGATCTCTCAATTGCTTTTTTTAATAAAAAAATAACTTATCTTTTCATTTAAAATGAGAGAAAAAAATATAATAACCAAATGATGCACTCACTTGGTTGATGAGTCAACTGAACTTCAAGTCACATGAACTCTATTTTTAAGGAATTGGAGAGTTAACAAAAATAAATACAATGAAGCATGTGAAAAAAATAGGGGTTGGACCGAAATATAGATATAGCTAAAGTTGTGAGTGCATGTCTCTTAATGAAAGTGTGTTTCATTAATATCATGTTTAATTAACGTTTAATATTTTTTTGGATGTGTGACAGTCAATGAAATTTGATTACATTACCAATGGGTATATACATCATATGCATTCTTTCAACAGCAAGCAGATTCATTTTGGGCTGTATGAATGTAGAAACATGACACTCACAAAACTACAAATAAAAGCCCCCGGGGATAGCCCCAATACAGATGGCATCAAAATAGGAAAGTCAAGGGGTATAAATATCACAAGTGTTGACATTAGAACGGGCGATGACTGCATTTCTATGCTCTCGGGTTTAAGCAATGTTCGAATTATGGATGTGTATTGTGGTCCTGGTCATGGAATAAGCATCGGAAGTCTTGGTAAGTACGAAAACGAAGAAGATCTTGCCGACATAATTGTTAAGAATTGCACATTGAATGGTACGAGTAATGGAGTCAGAATAAAATCATATGAGTCACAACTGAACAAAACCGTGGTTGCTTCTAATTTCATATATGAGGATATTGTGATGGAAAATGTGGAGCATCCCATTGTAATTGATCAACACTATTGTCCGAATTCTCTATGCCGCAAAAAGGTTTGTTTTGGGTTTCTCTAAATTAGTGAAAGAGGTTTTAATTATTAATTAAAAAATACCATATATTGATGATAGTTTTATGTACCTGCCAATAATTGTGTAGGGGGTTTCAAGCGTACAAATACGCAACGTGGCTTACAAATACATTAGAGGGAGTGGAAATTCAAAATTTGCAGCAACTTTCAACTGCATTCAAAGCAAACCATGCCAAAACATCACAATGGAGAATATCGATTTGTGGCCTTCTAGTACTAATGGAAGGCTACGAAATGAGTGTTCATTTGTAAGTGGAGCTGTTTATGGGAAACAAAATCCCCCTGCTTGCATAAAATAG
Protein sequence

>MS.gene63570.t1

MKAFLNAWRDACKWSGKSTVLIPNGTYKLKDVNFTGPCNSSINFRLEGMLKAPIDPNSFASDNWINFKHVNKLMVGGGGSLDGQGAYAWKMNDCKKNPNCRALPTSMKFDYITNGYIHHMHSFNSKQIHFGLYECRNMTLTKLQIKAPGDSPNTDGIKIGKSRGINITSVDIRTGDDCISMLSGLSNVRIMDVYCGPGHGISIGSLGKYENEEDLADIIVKNCTLNGTSNGVRIKSYESQLNKTVVASNFIYEDIVMENVEHPIVIDQHYCPNSLCRKKGVSSVQIRNVAYKYIRGSGNSKFAATFNCIQSKPCQNITMENIDLWPSSTNGRLRNECSFVSGAVYGKQNPPACIK