AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056777

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056777.t1 MTR_8g030930 89.223 399 39 2 1 395 1 399 0.0 700
MS.gene056777.t1 MTR_3g438050 63.384 396 142 3 1 394 1 395 0.0 516
MS.gene056777.t1 MTR_5g063430 71.061 311 85 1 1 311 1 306 3.34e-168 473
MS.gene056777.t1 MTR_1g088160 41.953 379 206 7 24 394 34 406 7.56e-99 300
MS.gene056777.t1 MTR_2g058670 37.913 393 232 6 6 390 6 394 5.35e-98 298
MS.gene056777.t1 MTR_2g058840 37.531 397 226 6 6 390 6 392 2.84e-97 296
MS.gene056777.t1 MTR_2g058640 37.755 392 232 6 7 390 7 394 2.03e-93 286
MS.gene056777.t1 MTR_4g083660 40.642 374 210 7 25 394 9 374 2.29e-90 277
MS.gene056777.t1 MTR_2g049400 38.776 392 226 8 5 394 12 391 2.90e-89 275
MS.gene056777.t1 MTR_2g435640 37.766 376 225 6 23 393 51 422 2.94e-88 273
MS.gene056777.t1 MTR_5g097860 43.125 320 176 4 21 337 21 337 9.22e-87 267
MS.gene056777.t1 MTR_1g052315 40.779 385 212 10 8 382 46 424 1.04e-86 270
MS.gene056777.t1 MTR_7g091850 40.793 353 198 7 18 364 69 416 1.72e-85 267
MS.gene056777.t1 MTR_4g120320 36.340 377 229 6 23 393 50 421 8.39e-83 259
MS.gene056777.t1 MTR_7g091840 41.250 320 177 6 23 335 63 378 1.41e-80 252
MS.gene056777.t1 MTR_6g028080 39.295 369 197 9 25 377 30 387 2.36e-80 252
MS.gene056777.t1 MTR_4g084050 41.067 375 196 9 4 364 2 365 3.09e-80 252
MS.gene056777.t1 MTR_2g060350 38.624 378 214 10 24 390 73 443 1.05e-79 252
MS.gene056777.t1 MTR_2g103410 36.605 377 226 8 24 393 58 428 2.30e-79 251
MS.gene056777.t1 MTR_8g009810 36.896 393 236 8 5 393 15 399 2.28e-78 247
MS.gene056777.t1 MTR_3g030540 40.845 355 199 7 15 364 60 408 3.96e-77 245
MS.gene056777.t1 MTR_8g065030 34.133 375 234 7 24 392 64 431 5.83e-77 245
MS.gene056777.t1 MTR_3g084380 40.401 349 188 8 25 364 21 358 2.72e-75 239
MS.gene056777.t1 MTR_3g054320 38.338 373 199 9 11 366 7 365 1.57e-73 235
MS.gene056777.t1 MTR_3g084360 38.462 377 210 9 6 371 3 368 1.86e-73 234
MS.gene056777.t1 MTR_5g043350 38.356 365 202 9 4 354 2 357 1.34e-72 232
MS.gene056777.t1 MTR_8g010080 35.884 379 221 7 5 377 7 369 2.97e-71 228
MS.gene056777.t1 MTR_8g010110 36.631 374 218 6 7 377 13 370 3.06e-71 228
MS.gene056777.t1 MTR_4g094715 34.197 386 235 8 24 395 64 444 3.90e-71 230
MS.gene056777.t1 MTR_8g010070 35.677 384 227 8 5 384 11 378 2.30e-70 226
MS.gene056777.t1 MTR_2g029170 38.643 339 200 6 58 394 29 361 3.40e-70 225
MS.gene056777.t1 MTR_6g005630 35.450 378 222 11 5 370 17 384 8.50e-70 226
MS.gene056777.t1 MTR_1g086390 34.726 383 230 9 24 391 80 457 6.58e-68 222
MS.gene056777.t1 MTR_5g033680 34.204 383 235 9 21 392 55 431 1.30e-67 221
MS.gene056777.t1 MTR_0157s0060 39.155 355 189 11 29 372 2 340 4.63e-67 217
MS.gene056777.t1 MTR_4g034020 39.155 355 189 11 29 372 2 340 4.63e-67 217
MS.gene056777.t1 MTR_2g100040 35.376 359 181 6 7 363 8 317 1.01e-66 216
MS.gene056777.t1 MTR_2g103650 33.246 382 242 7 5 377 8 385 1.15e-66 217
MS.gene056777.t1 MTR_7g110050 34.367 387 232 11 24 394 66 446 2.87e-66 218
MS.gene056777.t1 MTR_8g008765 36.261 353 203 6 5 350 7 344 3.59e-66 214
MS.gene056777.t1 MTR_8g010090 35.014 357 213 6 24 377 30 370 7.48e-66 214
MS.gene056777.t1 MTR_8g010040 34.831 356 212 7 25 377 32 370 8.36e-62 204
MS.gene056777.t1 MTR_5g034090 33.244 373 214 8 24 366 120 487 2.58e-61 206
MS.gene056777.t1 MTR_1g012390 32.737 391 250 9 6 391 9 391 2.69e-61 203
MS.gene056777.t1 MTR_1g013730 32.812 384 231 9 6 384 8 369 1.33e-59 198
MS.gene056777.t1 MTR_8g010050 41.304 276 151 4 5 276 11 279 7.17e-58 193
MS.gene056777.t1 MTR_7g035415 33.645 321 188 8 5 310 10 320 1.68e-57 191
MS.gene056777.t1 MTR_1g013740 31.061 396 245 9 5 394 15 388 2.68e-57 192
MS.gene056777.t1 MTR_2g103410 36.727 275 167 4 24 292 58 331 2.93e-57 191
MS.gene056777.t1 MTR_8g065010 31.367 373 220 8 25 366 107 474 3.79e-56 192
MS.gene056777.t1 MTR_6g005630 33.333 378 207 13 5 370 17 361 1.69e-55 188
MS.gene056777.t1 MTR_2g032710 32.493 357 221 7 24 366 66 416 8.62e-55 187
MS.gene056777.t1 MTR_7g113910 35.987 314 161 10 85 393 74 352 1.82e-54 184
MS.gene056777.t1 MTR_8g077320 34.194 310 160 6 15 310 23 302 1.08e-48 169
MS.gene056777.t1 MTR_5g034090 33.559 295 166 5 24 288 120 414 3.48e-47 166
MS.gene056777.t1 MTR_8g008700 34.375 288 170 5 84 367 14 286 2.98e-44 155
MS.gene056777.t1 MTR_8g031180 31.525 295 166 6 85 372 15 280 1.81e-35 132
MS.gene056777.t1 MTR_7g072360 58.182 110 24 1 167 276 41 128 3.57e-32 118
MS.gene056777.t1 MTR_7g072370 75.806 62 15 0 221 282 1 62 1.78e-24 95.5
MS.gene056777.t1 MTR_7g078680 23.116 398 264 10 26 391 56 443 4.01e-18 86.3
MS.gene056777.t1 MTR_4g124980 24.020 408 256 14 24 393 73 464 1.53e-17 84.7
MS.gene056777.t1 MTR_2g042740 23.325 403 265 13 24 393 55 446 1.09e-16 82.0
MS.gene056777.t1 MTR_8g098840 22.864 398 265 11 26 391 45 432 1.11e-15 79.0
MS.gene056777.t1 MTR_1g100050 23.196 388 260 10 27 384 52 431 3.76e-15 77.4
MS.gene056777.t1 MTR_8g028725 22.892 415 276 11 7 391 43 443 6.77e-15 76.6
MS.gene056777.t1 MTR_1g100060 22.727 396 252 12 27 384 95 474 9.71e-15 76.3
MS.gene056777.t1 MTR_1g100040 23.096 394 253 10 27 384 58 437 1.61e-13 72.4
MS.gene056777.t1 MTR_4g120730 22.751 378 236 13 26 364 41 401 3.37e-13 71.2
MS.gene056777.t1 MTR_1g099850 22.500 440 253 12 8 384 22 436 4.05e-13 70.9
MS.gene056777.t1 MTR_3g449590 24.242 429 257 14 15 393 37 447 6.15e-13 70.5
MS.gene056777.t1 MTR_5g090160 22.328 421 276 13 11 393 28 435 1.74e-11 65.9
MS.gene056777.t1 MTR_7g110710 24.165 389 225 13 26 358 44 418 2.58e-11 65.5
MS.gene056777.t1 MTR_7g110710 23.969 388 227 12 26 358 44 418 2.80e-11 65.5
MS.gene056777.t1 MTR_7g088550 22.807 399 246 14 15 367 33 415 3.54e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056777.t1 AT1G78400 51.206 373 181 1 23 395 33 404 2.53e-136 395
MS.gene056777.t1 AT2G15450 49.235 392 194 4 7 394 13 403 1.05e-131 384
MS.gene056777.t1 AT1G17150 47.301 389 202 2 8 394 14 401 1.09e-130 381
MS.gene056777.t1 AT1G43080 49.618 393 189 6 9 394 13 403 1.39e-130 381
MS.gene056777.t1 AT1G43090 50.130 385 185 5 9 387 13 396 6.08e-130 381
MS.gene056777.t1 AT2G33160 47.643 403 200 5 1 393 1 402 4.30e-129 386
MS.gene056777.t1 AT2G15460 48.969 388 193 4 7 390 13 399 1.14e-128 376
MS.gene056777.t1 AT2G15470 48.724 392 196 4 7 394 13 403 1.78e-128 375
MS.gene056777.t1 AT4G13760 48.223 394 195 5 5 394 15 403 2.44e-128 375
MS.gene056777.t1 AT1G43100 50.259 386 183 6 9 387 13 396 6.48e-127 373
MS.gene056777.t1 AT2G40310 47.727 396 201 5 4 394 9 403 2.73e-125 367
MS.gene056777.t1 AT2G26620 47.792 385 198 3 12 394 18 401 6.10e-123 362
MS.gene056777.t1 AT5G48140 39.949 393 224 4 1 393 6 386 4.58e-102 308
MS.gene056777.t1 AT3G07820 38.422 393 235 3 1 393 1 386 1.69e-101 306
MS.gene056777.t1 AT3G07840 40.464 388 220 5 6 393 11 387 1.79e-100 304
MS.gene056777.t1 AT2G43890 42.091 373 204 8 25 394 29 392 1.13e-99 301
MS.gene056777.t1 AT4G18180 40.260 385 214 6 24 393 38 421 2.75e-99 302
MS.gene056777.t1 AT3G07830 38.422 393 232 3 1 393 5 387 1.46e-97 296
MS.gene056777.t1 AT1G05650 38.520 392 232 6 5 394 8 392 1.40e-95 291
MS.gene056777.t1 AT1G80170 39.791 382 218 7 20 394 48 424 8.80e-95 291
MS.gene056777.t1 AT3G14040 40.731 383 221 4 15 395 61 439 5.07e-93 286
MS.gene056777.t1 AT1G05660 37.659 393 236 6 4 394 7 392 3.09e-92 283
MS.gene056777.t1 AT3G07850 41.287 373 213 4 25 395 70 438 1.43e-91 283
MS.gene056777.t1 AT2G43870 39.846 389 219 10 6 390 4 381 1.38e-88 273
MS.gene056777.t1 AT2G43880 38.814 371 219 5 25 394 31 394 2.94e-88 273
MS.gene056777.t1 AT3G07970 40.584 377 214 7 19 390 65 436 1.44e-87 272
MS.gene056777.t1 AT3G59850 40.160 376 213 8 23 394 21 388 1.95e-87 270
MS.gene056777.t1 AT3G59850 40.160 376 213 8 23 394 65 432 4.93e-87 271
MS.gene056777.t1 AT3G57510 40.000 375 213 8 8 376 52 420 1.94e-85 266
MS.gene056777.t1 AT2G41850 39.370 381 218 8 15 389 58 431 2.87e-85 266
MS.gene056777.t1 AT1G02790 36.649 382 221 5 1 370 16 388 2.08e-83 261
MS.gene056777.t1 AT2G43860 40.282 355 196 9 25 370 33 380 8.20e-80 251
MS.gene056777.t1 AT1G65570 36.000 400 237 12 5 394 7 397 5.09e-76 241
MS.gene056777.t1 AT4G35670 37.203 379 221 8 23 395 22 389 1.95e-75 239
MS.gene056777.t1 AT2G43880 41.522 289 163 4 25 312 31 314 2.16e-75 237
MS.gene056777.t1 AT1G70500 33.333 375 237 7 24 392 66 433 1.59e-74 239
MS.gene056777.t1 AT3G15720 37.330 367 212 7 25 384 24 379 5.21e-73 235
MS.gene056777.t1 AT3G26610 37.110 353 208 7 24 368 66 412 5.53e-73 235
MS.gene056777.t1 AT3G15720 37.330 367 212 7 25 384 23 378 6.63e-73 235
MS.gene056777.t1 AT1G23460 33.067 375 238 7 24 392 42 409 1.25e-72 233
MS.gene056777.t1 AT1G23460 33.067 375 238 7 24 392 66 433 2.39e-72 233
MS.gene056777.t1 AT5G17200 33.645 428 231 9 1 388 1 415 4.11e-71 229
MS.gene056777.t1 AT1G02460 34.884 387 227 10 24 391 88 468 1.12e-69 228
MS.gene056777.t1 AT3G15720 37.057 367 210 8 25 384 23 375 1.13e-69 226
MS.gene056777.t1 AT5G27530 37.249 349 193 8 23 364 51 380 6.43e-69 224
MS.gene056777.t1 AT5G14650 35.979 378 226 12 25 395 51 419 3.55e-68 222
MS.gene056777.t1 AT4G32380 34.328 402 241 9 5 393 3 394 5.96e-68 222
MS.gene056777.t1 AT1G80170 41.509 265 148 4 20 278 48 311 1.24e-67 217
MS.gene056777.t1 AT5G44840 33.333 396 245 8 6 395 8 390 1.55e-67 219
MS.gene056777.t1 AT4G01890 33.791 364 214 11 24 367 64 420 3.47e-65 215
MS.gene056777.t1 AT1G10640 31.000 400 238 7 23 392 117 508 9.58e-64 213
MS.gene056777.t1 AT1G80140 36.232 345 200 8 58 393 2 335 1.41e-63 207
MS.gene056777.t1 AT1G48100 33.427 356 219 8 24 366 79 429 1.40e-62 208
MS.gene056777.t1 AT1G60590 31.467 375 219 7 25 366 123 492 1.71e-62 210
MS.gene056777.t1 AT1G56710 32.902 386 239 8 24 394 47 427 9.24e-62 205
MS.gene056777.t1 AT3G15720 36.615 325 189 6 66 384 2 315 3.71e-61 202
MS.gene056777.t1 AT4G32370 37.231 325 172 12 5 310 11 322 1.08e-57 192
MS.gene056777.t1 AT4G32375 35.417 288 176 3 99 385 8 286 1.34e-57 194
MS.gene056777.t1 AT5G39910 33.819 343 190 9 1 328 6 326 7.55e-56 188
MS.gene056777.t1 AT5G44830 34.405 311 180 6 59 364 10 301 9.93e-55 184
MS.gene056777.t1 AT4G32380 30.750 400 217 8 5 393 3 353 1.91e-54 186
MS.gene056777.t1 AT4G35670 36.519 293 173 5 104 395 2 282 2.54e-54 181
MS.gene056777.t1 AT5G14650 37.066 259 154 7 25 278 51 305 1.29e-48 167
MS.gene056777.t1 AT4G23820 24.297 391 241 11 15 367 31 404 8.64e-18 85.1
MS.gene056777.t1 AT1G19170 23.342 407 261 12 24 393 86 478 1.07e-17 85.1
MS.gene056777.t1 AT3G48950 21.921 406 261 11 27 393 47 435 1.60e-15 78.6
MS.gene056777.t1 AT3G61490 21.968 437 276 12 6 391 11 433 3.41e-15 77.4
MS.gene056777.t1 AT3G61490 21.968 437 276 12 6 391 11 433 3.41e-15 77.4
MS.gene056777.t1 AT3G61490 21.968 437 276 12 6 391 11 433 3.41e-15 77.4
MS.gene056777.t1 AT3G61490 21.968 437 276 12 6 391 26 448 3.70e-15 77.4
MS.gene056777.t1 AT3G61490 21.968 437 276 12 6 391 124 546 9.89e-15 76.3
MS.gene056777.t1 AT3G62110 23.786 412 251 13 15 384 34 424 1.26e-14 75.9
MS.gene056777.t1 AT3G62110 23.786 412 251 13 15 384 34 424 1.26e-14 75.9
MS.gene056777.t1 AT5G41870 23.245 413 268 13 15 393 35 432 2.02e-14 75.1
MS.gene056777.t1 AT2G23900 22.963 405 258 13 27 393 58 446 3.73e-12 68.2
MS.gene056777.t1 AT4G23500 22.362 398 267 11 26 391 69 456 2.17e-11 65.9

Find 68 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGTCATTTCGTATTATT+TGG 0.045891 8.4:-66274295 MS.gene056777:CDS
ATTCATTTAAGACTGATAAT+TGG 0.248897 8.4:-66275184 MS.gene056777:CDS
ATCTGTGTTGGGGCTATCAT+TGG 0.279672 8.4:+66274231 None:intergenic
TTATCAGTCTTAAATGAATA+TGG 0.288964 8.4:+66275188 None:intergenic
GATATTAATTTGCGGCCTTC+TGG 0.303523 8.4:-66273605 MS.gene056777:CDS
TCTGTGTTGGGGCTATCATT+GGG 0.307341 8.4:+66274232 None:intergenic
TTAAGCATATATGTCCCTTC+TGG 0.320627 8.4:+66275287 None:intergenic
CAATGTGTGTTTATTTGTTA+AGG 0.321877 8.4:-66273564 MS.gene056777:CDS
TACTAGGAATGTTCGAATTT+CGG 0.331851 8.4:-66274122 MS.gene056777:CDS
AACACAGATGGTATCAAAAT+AGG 0.359408 8.4:-66274217 MS.gene056777:CDS
GTTAAGAATTGTACCTTTAG+TGG 0.375278 8.4:-66274016 MS.gene056777:CDS
CTTAAGGAAGTTGTATTTAG+TGG 0.376887 8.4:-66275269 MS.gene056777:CDS
CCCCTTCTTGCATAAAATAG+TGG 0.382661 8.4:-66273517 None:intergenic
ACGTCCTCCGTGCACCAAAA+AGG 0.391567 8.4:-66273858 MS.gene056777:intron
AAAGTCATTTCGTATTATTT+GGG 0.397273 8.4:-66274294 MS.gene056777:CDS
TGGATGAACTTTAAAATTGA+AGG 0.400013 8.4:-66275233 MS.gene056777:CDS
TTGCTATGCTCTCGGGTACT+AGG 0.400268 8.4:-66274138 MS.gene056777:CDS
CATTGAAAAGCTTGTATTGA+CGG 0.403752 8.4:+66275528 None:intergenic
AATGTGTGTTTATTTGTTAA+GGG 0.428144 8.4:-66273563 MS.gene056777:CDS
TAAAATTACCACACTATTGT+CGG 0.450350 8.4:+66275472 None:intergenic
TTTGTTAAGGGTGCTAGTTA+TGG 0.458228 8.4:-66273551 MS.gene056777:CDS
GGAATTAGTGTCGGAAGTCT+TGG 0.461747 8.4:-66274076 MS.gene056777:CDS
GTCTCAATTGAAGAAAACTT+TGG 0.463231 8.4:-66273960 MS.gene056777:CDS
ATCAAAATAGGAAGGTCAAA+TGG 0.467776 8.4:-66274205 MS.gene056777:CDS
ACCTTTAGTGGCACAAGTAA+TGG 0.469662 8.4:-66274004 MS.gene056777:CDS
AGAAGGGACATATATGCTTA+AGG 0.469662 8.4:-66275285 MS.gene056777:CDS
TAAGCATATATGTCCCTTCT+GGG 0.477245 8.4:+66275288 None:intergenic
CTGTGTTGGGGCTATCATTG+GGG 0.485147 8.4:+66274233 None:intergenic
GGTGGAGGTGGCAGATTGGA+TGG 0.495038 8.4:-66275128 MS.gene056777:CDS
GATTGCATTGCTATGCTCTC+GGG 0.503203 8.4:-66274145 MS.gene056777:CDS
TCAAGTGTGAACATTGGAAC+CGG 0.504301 8.4:-66274172 MS.gene056777:CDS
TAATCTTGATCAATGACAAT+GGG 0.504846 8.4:+66273887 None:intergenic
AATTATGGTGCACGTTCTGA+TGG 0.506363 8.4:-66275500 MS.gene056777:CDS
ATGGTCAAGGATCTTCTGCT+TGG 0.509723 8.4:-66275109 MS.gene056777:CDS
TGATTGCATTGCTATGCTCT+CGG 0.518127 8.4:-66274146 MS.gene056777:CDS
AAATTGACTGTTGGTGGAGG+TGG 0.527277 8.4:-66275140 MS.gene056777:CDS
ACCATTACTTGTGCCACTAA+AGG 0.530125 8.4:+66274003 None:intergenic
AAATTTGACTTCATCACCAA+TGG 0.535321 8.4:-66274352 MS.gene056777:CDS
AAGTTCATCCAAGCATTGCA+TGG 0.537683 8.4:+66275245 None:intergenic
TTAAATGAATATGGATCACT+TGG 0.537771 8.4:+66275197 None:intergenic
TTAGTGGTCCATGCAATGCT+TGG 0.539885 8.4:-66275253 MS.gene056777:CDS
GAGTGGAGGATATTAATTTG+CGG 0.539974 8.4:-66273613 MS.gene056777:CDS
AATATTTCAAGTGTGAACAT+TGG 0.546960 8.4:-66274178 MS.gene056777:CDS
TGTTGGTGGAGGTGGCAGAT+TGG 0.550018 8.4:-66275132 MS.gene056777:CDS
GTTAACAAATTGACTGTTGG+TGG 0.553903 8.4:-66275146 MS.gene056777:CDS
GGAAGTCTTGGTAAGAATGA+AGG 0.566088 8.4:-66274064 MS.gene056777:CDS
AACAAATTGACTGTTGGTGG+AGG 0.571472 8.4:-66275143 MS.gene056777:CDS
CATGGAGTGATGCATGCAGA+TGG 0.579101 8.4:-66275334 MS.gene056777:CDS
TGTGTTGGGGCTATCATTGG+GGG 0.592002 8.4:+66274234 None:intergenic
AGTGATGCATGCAGATGGAA+TGG 0.594601 8.4:-66275329 MS.gene056777:CDS
CATAGCAATGCAATCATCAC+CGG 0.595285 8.4:+66274153 None:intergenic
TATGTATGAAGATATTGTGA+TGG 0.595589 8.4:-66273924 MS.gene056777:CDS
ATAATCTTGATCAATGACAA+TGG 0.600281 8.4:+66273886 None:intergenic
CAATGGTTATGTCCATCACA+TGG 0.600984 8.4:-66274335 MS.gene056777:CDS
AATGATAGCCCCAACACAGA+TGG 0.606825 8.4:-66274229 MS.gene056777:CDS
GGTGGCAGATTGGATGGTCA+AGG 0.607840 8.4:-66275122 MS.gene056777:CDS
TGGTGTTAGAATCAAATCGT+GGG 0.618282 8.4:-66273984 MS.gene056777:CDS
CCTGGTCATGGAATTAGTGT+CGG 0.621889 8.4:-66274085 MS.gene056777:CDS
ACCATGTCAAAACATTAGAG+TGG 0.622168 8.4:-66273630 MS.gene056777:CDS
CCGACACTAATTCCATGACC+AGG 0.625972 8.4:+66274085 None:intergenic
TATGTTAACAAATTGACTGT+TGG 0.627069 8.4:-66275149 MS.gene056777:CDS
ATGGTGTTAGAATCAAATCG+TGG 0.632554 8.4:-66273985 MS.gene056777:CDS
TGTTGATAGATTCCATGTGA+TGG 0.635321 8.4:+66274323 None:intergenic
CAGATGGTATCAAAATAGGA+AGG 0.635447 8.4:-66274213 MS.gene056777:CDS
ATGTCAAAACATTAGAGTGG+AGG 0.635960 8.4:-66273627 MS.gene056777:CDS
TGGAAATACCGACAATAGTG+TGG 0.643099 8.4:-66275480 MS.gene056777:intron
CATGTGATGGACATAACCAT+TGG 0.662903 8.4:+66274336 None:intergenic
AAGTGTACAAATAAGCAATG+TGG 0.668805 8.4:-66273720 MS.gene056777:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTATTAAAAATTAA+GGG + chr8.4:66274144-66274163 None:intergenic 0.0%
!!! TATTTTTTTATTAAAAATTA+AGG + chr8.4:66274145-66274164 None:intergenic 0.0%
!!! TTAAAATTTTATATATTTTA+TGG - chr8.4:66274709-66274728 MS.gene056777:intron 0.0%
!! TAATTATTCTAATATACAAA+AGG - chr8.4:66274567-66274586 MS.gene056777:intron 10.0%
!! AAATTAAAAGAAAAATGAGA+AGG + chr8.4:66273668-66273687 None:intergenic 15.0%
!!! ATATATTTTATGGATATGTT+TGG - chr8.4:66274719-66274738 MS.gene056777:intron 15.0%
!!! TATCATGATTTTGATTTTAA+TGG + chr8.4:66274437-66274456 None:intergenic 15.0%
!! AAACTTATAAAAGACTAAGT+TGG + chr8.4:66275313-66275332 None:intergenic 20.0%
!! AAAGTCATTTCGTATTATTT+GGG - chr8.4:66274810-66274829 MS.gene056777:intron 20.0%
!! AACTTATAAAAGACTAAGTT+GGG + chr8.4:66275312-66275331 None:intergenic 20.0%
!! ATATAAAATGAACTTACAGT+AGG + chr8.4:66274063-66274082 None:intergenic 20.0%
!! GAGAGGAAAAAAAATATAAA+TGG - chr8.4:66274186-66274205 MS.gene056777:CDS 20.0%
!! GTTAACAAAAAAAAACGATA+AGG - chr8.4:66274300-66274319 MS.gene056777:CDS 20.0%
!! TGAAATAAATCATCAAACAT+TGG - chr8.4:66274467-66274486 MS.gene056777:intron 20.0%
!! TTATCAGTCTTAAATGAATA+TGG + chr8.4:66273919-66273938 None:intergenic 20.0%
!!! AATGTGTGTTTATTTGTTAA+GGG - chr8.4:66275541-66275560 MS.gene056777:CDS 20.0%
!!! AGTTTGTTATTTTCTTATGA+AGG - chr8.4:66273735-66273754 MS.gene056777:intron 20.0%
!!! ATTCATTTAAGACTGATAAT+TGG - chr8.4:66273920-66273939 MS.gene056777:CDS 20.0%
!!! CTTATTTCTTAACTAAATAG+TGG + chr8.4:66274530-66274549 None:intergenic 20.0%
! ATAATCTTGATCAATGACAA+TGG + chr8.4:66275221-66275240 None:intergenic 25.0%
! CAAAGTCATTTCGTATTATT+TGG - chr8.4:66274809-66274828 MS.gene056777:intron 25.0%
! TAAAATTACCACACTATTGT+CGG + chr8.4:66273635-66273654 None:intergenic 25.0%
! TAATCTTGATCAATGACAAT+GGG + chr8.4:66275220-66275239 None:intergenic 25.0%
! TACAAAAATATTAGAGGAAG+TGG - chr8.4:66275409-66275428 MS.gene056777:intron 25.0%
! TATGTATGAAGATATTGTGA+TGG - chr8.4:66275180-66275199 MS.gene056777:CDS 25.0%
! TATGTTAACAAATTGACTGT+TGG - chr8.4:66273955-66273974 MS.gene056777:CDS 25.0%
! TTAAATGAATATGGATCACT+TGG + chr8.4:66273910-66273929 None:intergenic 25.0%
!! AATACATGTACATACCTTTT+TGG + chr8.4:66275263-66275282 None:intergenic 25.0%
!! AATATTTCAAGTGTGAACAT+TGG - chr8.4:66274926-66274945 MS.gene056777:intron 25.0%
!! ATTACTAAAGCTCATTTTGT+TGG - chr8.4:66274268-66274287 MS.gene056777:CDS 25.0%
!! CAATGTGTGTTTATTTGTTA+AGG - chr8.4:66275540-66275559 MS.gene056777:CDS 25.0%
!! CTTTTCAATGTGAAGAATTA+TGG - chr8.4:66273589-66273608 MS.gene056777:CDS 25.0%
!! GTCATGTTTAATTAGTTTCT+AGG - chr8.4:66274397-66274416 MS.gene056777:intron 25.0%
!! TCTGACAATAATTTTGTGTA+GGG - chr8.4:66275355-66275374 MS.gene056777:intron 25.0%
!! TTACTAAAGCTCATTTTGTT+GGG - chr8.4:66274269-66274288 MS.gene056777:CDS 25.0%
!! TTCTGACAATAATTTTGTGT+AGG - chr8.4:66275354-66275373 MS.gene056777:intron 25.0%
!!! ATCGTTTTATTCAAAATGAG+AGG - chr8.4:66274169-66274188 MS.gene056777:CDS 25.0%
!!! TGATTTTGATTTTAATGGTG+AGG + chr8.4:66274432-66274451 None:intergenic 25.0%
!!! TGGATGAACTTTAAAATTGA+AGG - chr8.4:66273871-66273890 MS.gene056777:CDS 25.0%
AAAAACGATAAGGTAGAGTA+TGG - chr8.4:66274310-66274329 MS.gene056777:CDS 30.0%
AACACAGATGGTATCAAAAT+AGG - chr8.4:66274887-66274906 MS.gene056777:intron 30.0%
AAGTGTACAAATAAGCAATG+TGG - chr8.4:66275384-66275403 MS.gene056777:intron 30.0%
AATACCCTATGAAATTGATG+TGG - chr8.4:66274092-66274111 MS.gene056777:CDS 30.0%
AGTATGGAAAAAGTAGAGAT+TGG - chr8.4:66274326-66274345 MS.gene056777:CDS 30.0%
ATCAAAATAGGAAGGTCAAA+TGG - chr8.4:66274899-66274918 MS.gene056777:intron 30.0%
CATTGAAAAGCTTGTATTGA+CGG + chr8.4:66273579-66273598 None:intergenic 30.0%
CTTAAGGAAGTTGTATTTAG+TGG - chr8.4:66273835-66273854 MS.gene056777:intron 30.0%
GTCTCAATTGAAGAAAACTT+TGG - chr8.4:66275144-66275163 MS.gene056777:CDS 30.0%
GTGGCTTACAAAAATATTAG+AGG - chr8.4:66275403-66275422 MS.gene056777:intron 30.0%
GTTAAGAATTGTACCTTTAG+TGG - chr8.4:66275088-66275107 MS.gene056777:CDS 30.0%
TACTAGGAATGTTCGAATTT+CGG - chr8.4:66274982-66275001 MS.gene056777:intron 30.0%
! AAATTTGACTTCATCACCAA+TGG - chr8.4:66274752-66274771 MS.gene056777:intron 30.0%
! CTGACAATAATTTTGTGTAG+GGG - chr8.4:66275356-66275375 MS.gene056777:intron 30.0%
! GAGATAGAGTGTTTGTATTT+CGG + chr8.4:66274353-66274372 None:intergenic 30.0%
! TGACAATAATTTTGTGTAGG+GGG - chr8.4:66275357-66275376 MS.gene056777:intron 30.0%
!! ATTTTGATACCATCTGTGTT+GGG + chr8.4:66274887-66274906 None:intergenic 30.0%
!!! TATTTTGATACCATCTGTGT+TGG + chr8.4:66274888-66274907 None:intergenic 30.0%
!!! TGAAGGCATTTTTAAAAGCA+TGG - chr8.4:66273752-66273771 MS.gene056777:intron 30.0%
ACCATGTCAAAACATTAGAG+TGG - chr8.4:66275474-66275493 MS.gene056777:intron 35.0%
AGAAGGGACATATATGCTTA+AGG - chr8.4:66273819-66273838 MS.gene056777:intron 35.0%
ATCTGCCACATCAATTTCAT+AGG + chr8.4:66274100-66274119 None:intergenic 35.0%
ATGTCAAAACATTAGAGTGG+AGG - chr8.4:66275477-66275496 MS.gene056777:intron 35.0%
CAGATGGTATCAAAATAGGA+AGG - chr8.4:66274891-66274910 MS.gene056777:intron 35.0%
GGTCAAAAGAACAAGCATTA+AGG + chr8.4:66274509-66274528 None:intergenic 35.0%
GTTAACAAATTGACTGTTGG+TGG - chr8.4:66273958-66273977 MS.gene056777:CDS 35.0%
TAAGCATATATGTCCCTTCT+GGG + chr8.4:66273819-66273838 None:intergenic 35.0%
TCTGCCACATCAATTTCATA+GGG + chr8.4:66274099-66274118 None:intergenic 35.0%
TTAAGCATATATGTCCCTTC+TGG + chr8.4:66273820-66273839 None:intergenic 35.0%
! ATGGTGTTAGAATCAAATCG+TGG - chr8.4:66275119-66275138 MS.gene056777:CDS 35.0%
! CTCATTTTGTTGGGAATTGA+GGG - chr8.4:66274278-66274297 MS.gene056777:CDS 35.0%
! GAGTGGAGGATATTAATTTG+CGG - chr8.4:66275491-66275510 MS.gene056777:CDS 35.0%
! TGGTGTTAGAATCAAATCGT+GGG - chr8.4:66275120-66275139 MS.gene056777:CDS 35.0%
! TGTTGATAGATTCCATGTGA+TGG + chr8.4:66274784-66274803 None:intergenic 35.0%
! TTTTGATACCATCTGTGTTG+GGG + chr8.4:66274886-66274905 None:intergenic 35.0%
!! TTTGTTAAGGGTGCTAGTTA+TGG - chr8.4:66275553-66275572 MS.gene056777:CDS 35.0%
!!! CGAATTTCGGATGTTTTTTG+TGG - chr8.4:66274995-66275014 MS.gene056777:intron 35.0%
!!! TCCACTCTAATGTTTTGACA+TGG + chr8.4:66275478-66275497 None:intergenic 35.0%
AATTATGGTGCACGTTCTGA+TGG - chr8.4:66273604-66273623 MS.gene056777:CDS 40.0%
ACCTTTAGTGGCACAAGTAA+TGG - chr8.4:66275100-66275119 MS.gene056777:CDS 40.0%
CAATGGTTATGTCCATCACA+TGG - chr8.4:66274769-66274788 MS.gene056777:intron 40.0%
CATAGCAATGCAATCATCAC+CGG + chr8.4:66274954-66274973 None:intergenic 40.0%
GGAAGTCTTGGTAAGAATGA+AGG - chr8.4:66275040-66275059 MS.gene056777:intron 40.0%
TCAAGTGTGAACATTGGAAC+CGG - chr8.4:66274932-66274951 MS.gene056777:intron 40.0%
TGATTGCATTGCTATGCTCT+CGG - chr8.4:66274958-66274977 MS.gene056777:intron 40.0%
TGGAAATACCGACAATAGTG+TGG - chr8.4:66273624-66273643 MS.gene056777:CDS 40.0%
! AACAAATTGACTGTTGGTGG+AGG - chr8.4:66273961-66273980 MS.gene056777:CDS 40.0%
! AAGTTCATCCAAGCATTGCA+TGG + chr8.4:66273862-66273881 None:intergenic 40.0%
! ACCATTACTTGTGCCACTAA+AGG + chr8.4:66275104-66275123 None:intergenic 40.0%
! AGTCTTTTACTACGACCAGA+AGG + chr8.4:66275517-66275536 None:intergenic 40.0%
! CAACTGTTTTCATCCCAGAA+GGG - chr8.4:66273803-66273822 MS.gene056777:intron 40.0%
! GCTCATTTTGTTGGGAATTG+AGG - chr8.4:66274277-66274296 MS.gene056777:CDS 40.0%
!! CATGTGATGGACATAACCAT+TGG + chr8.4:66274771-66274790 None:intergenic 40.0%
!! GATATTAATTTGCGGCCTTC+TGG - chr8.4:66275499-66275518 MS.gene056777:CDS 40.0%
!! GTACATACCTTTTTGGTGCA+CGG + chr8.4:66275256-66275275 None:intergenic 40.0%
AATGATAGCCCCAACACAGA+TGG - chr8.4:66274875-66274894 MS.gene056777:intron 45.0%
ACAATGTGATTGAGCTCGAG+CGG - chr8.4:66274223-66274242 MS.gene056777:CDS 45.0%
AGTGATGCATGCAGATGGAA+TGG - chr8.4:66273775-66273794 MS.gene056777:intron 45.0%
ATCTGTGTTGGGGCTATCAT+TGG + chr8.4:66274876-66274895 None:intergenic 45.0%
ATGGTCAAGGATCTTCTGCT+TGG - chr8.4:66273995-66274014 MS.gene056777:CDS 45.0%
CCTGGTCATGGAATTAGTGT+CGG - chr8.4:66275019-66275038 MS.gene056777:intron 45.0%
GATTGCATTGCTATGCTCTC+GGG - chr8.4:66274959-66274978 MS.gene056777:intron 45.0%
TCTGTGTTGGGGCTATCATT+GGG + chr8.4:66274875-66274894 None:intergenic 45.0%
TTAGTGGTCCATGCAATGCT+TGG - chr8.4:66273851-66273870 MS.gene056777:intron 45.0%
! AAATTGACTGTTGGTGGAGG+TGG - chr8.4:66273964-66273983 MS.gene056777:CDS 45.0%
! GCAACTGTTTTCATCCCAGA+AGG - chr8.4:66273802-66273821 MS.gene056777:intron 45.0%
! GGAATTAGTGTCGGAAGTCT+TGG - chr8.4:66275028-66275047 MS.gene056777:intron 45.0%
! TGCAAGAAGGGGGATTTTGT+TGG + chr8.4:66275579-66275598 None:intergenic 45.0%
!!! GTTTTTTGTGGTCCTGGTCA+TGG - chr8.4:66275007-66275026 MS.gene056777:intron 45.0%
!!! TCGGATGTTTTTTGTGGTCC+TGG - chr8.4:66275001-66275020 MS.gene056777:intron 45.0%
!!! TGTTTTTTTTTTTTAAATAA+TGG + chr8.4:66274634-66274653 None:intergenic 5.0%
CATGGAGTGATGCATGCAGA+TGG - chr8.4:66273770-66273789 MS.gene056777:intron 50.0%
CCGACACTAATTCCATGACC+AGG + chr8.4:66275022-66275041 None:intergenic 50.0%
CTGTGTTGGGGCTATCATTG+GGG + chr8.4:66274874-66274893 None:intergenic 50.0%
TGTGTTGGGGCTATCATTGG+GGG + chr8.4:66274873-66274892 None:intergenic 50.0%
TTGCTATGCTCTCGGGTACT+AGG - chr8.4:66274966-66274985 MS.gene056777:intron 50.0%
!! CATACCTTTTTGGTGCACGG+AGG + chr8.4:66275253-66275272 None:intergenic 50.0%
ACGTCCTCCGTGCACCAAAA+AGG - chr8.4:66275246-66275265 MS.gene056777:CDS 55.0%
GGTGGCAGATTGGATGGTCA+AGG - chr8.4:66273982-66274001 MS.gene056777:CDS 55.0%
! TGTTGGTGGAGGTGGCAGAT+TGG - chr8.4:66273972-66273991 MS.gene056777:CDS 55.0%
!! TTTTTGGTGCACGGAGGACG+TGG + chr8.4:66275247-66275266 None:intergenic 55.0%
GGTGGAGGTGGCAGATTGGA+TGG - chr8.4:66273976-66273995 MS.gene056777:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 66273520 66275606 66273520 ID=MS.gene056777
chr8.4 mRNA 66273520 66275606 66273520 ID=MS.gene056777.t1;Parent=MS.gene056777
chr8.4 exon 66275481 66275606 66275481 ID=MS.gene056777.t1.exon1;Parent=MS.gene056777.t1
chr8.4 CDS 66275481 66275606 66275481 ID=cds.MS.gene056777.t1;Parent=MS.gene056777.t1
chr8.4 exon 66275061 66275369 66275061 ID=MS.gene056777.t1.exon2;Parent=MS.gene056777.t1
chr8.4 CDS 66275061 66275369 66275061 ID=cds.MS.gene056777.t1;Parent=MS.gene056777.t1
chr8.4 exon 66273859 66274380 66273859 ID=MS.gene056777.t1.exon3;Parent=MS.gene056777.t1
chr8.4 CDS 66273859 66274380 66273859 ID=cds.MS.gene056777.t1;Parent=MS.gene056777.t1
chr8.4 exon 66273520 66273750 66273520 ID=MS.gene056777.t1.exon4;Parent=MS.gene056777.t1
chr8.4 CDS 66273520 66273750 66273520 ID=cds.MS.gene056777.t1;Parent=MS.gene056777.t1
Gene Sequence

>MS.gene056777

ATGGGTGCAAAATTTTTGTTTGTGTTGAGCTTACTAGTTTGTATTGCAGAAGCACGCCGTCAATACAAGCTTTTCAATGTGAAGAATTATGGTGCACGTTCTGATGGAAATACCGACAATAGTGTGGTAATTTTACATGATCATTCCTTCTCATTTTTCTTTTAATTTCTTTTATTTTCATACAATTGCAATTTTGATTTTGATGAGCTAACTATAGTTTGTTATTTTCTTATGAAGGCATTTTTAAAAGCATGGAGTGATGCATGCAGATGGAATGGAAAAGCAACTGTTTTCATCCCAGAAGGGACATATATGCTTAAGGAAGTTGTATTTAGTGGTCCATGCAATGCTTGGATGAACTTTAAAATTGAAGGACTATTGAAAGCTCCAAGTGATCCATATTCATTTAAGACTGATAATTGGATAAATTTTAGATATGTTAACAAATTGACTGTTGGTGGAGGTGGCAGATTGGATGGTCAAGGATCTTCTGCTTGGAAAATTAATGATTGCAAAAAGAATTCAAATTGTCGCTCTCTTCCTACTGTAAGTTCATTTTATATATACAAATCAATACCCTATGAAATTGATGTGGCAGATATATTTTTAATTTTTTTTTATCCCTTAATTTTTAATAAAAAAATAACTTATCGTTTTATTCAAAATGAGAGGAAAAAAAATATAAATGGTAAAATATACAATAACAATGTGATTGAGCTCGAGCGGCTAATGAGTATATGAATTTCAGATTACTAAAGCTCATTTTGTTGGGAATTGAGGGTTAACAAAAAAAAACGATAAGGTAGAGTATGGAAAAAGTAGAGATTGGACCGAAATACAAACACTCTATCTCTTAATGAAAGTATGTTTCATTATTGTCATGTTTAATTAGTTTCTAGGATCACTTTGCCTCACCATTAAAATCAAAATCATGATAAAATCGAAATTGAAATAAATCATCAAACATTGGTATAGAAGTAAAACAACCTTAATGCTTGTTCTTTTGACCACTATTTAGTTAAGAAATAAGAAATGTAGAGTTTTTAATAATTATTCTAATATACAAAAGGTTACATTTTTTAAAACTCTACTTCTTTATACTAGTAAATTACCATTATTTAAAAAAAAAAAACATTTTTTTGATACTATAATTATTTTAGCAAGATAATGTTCGTTTCAGATGTGTTAATTAAAATTTTATATATTTTATGGATATGTTTGGACAGACAATGAAATTTGACTTCATCACCAATGGTTATGTCCATCACATGGAATCTATCAACAGCAAACAAAGTCATTTCGTATTATTTGGGTGTGAAAACATGACACTTAGAAGACTGAAAATAACAGCCCCCAATGATAGCCCCAACACAGATGGTATCAAAATAGGAAGGTCAAATGGAATAAATATTTCAAGTGTGAACATTGGAACCGGTGATGATTGCATTGCTATGCTCTCGGGTACTAGGAATGTTCGAATTTCGGATGTTTTTTGTGGTCCTGGTCATGGAATTAGTGTCGGAAGTCTTGGTAAGAATGAAGGTGAAGAAGATATTGATGACATTGTTGTTAAGAATTGTACCTTTAGTGGCACAAGTAATGGTGTTAGAATCAAATCGTGGGAGTCTCAATTGAAGAAAACTTTGGTTGTTTCGAATTTTATGTATGAAGATATTGTGATGGATAATGTGCAATATCCCATTGTCATTGATCAAGATTATTGTCCACGTCCTCCGTGCACCAAAAAGGTATGTACATGTATTCTACGTTCGAATATTATTCTTTTATACCCAACTTAGTCTTTTATAAGTTTGAGTTCTCTATAGACAATAATTTCTGACAATAATTTTGTGTAGGGGGTTTCAAGTGTACAAATAAGCAATGTGGCTTACAAAAATATTAGAGGAAGTGGAAATACAAAAGTTGCAGCAAGCTTCGAATGCAGCTCAAACAAACCATGTCAAAACATTAGAGTGGAGGATATTAATTTGCGGCCTTCTGGTCGTAGTAAAAGACTAAACAATGTGTGTTTATTTGTTAAGGGTGCTAGTTATGGCCAACAAAATCCCCCTTCTTGCATAAAATAG
Protein sequence

>MS.gene056777.t1

MGAKFLFVLSLLVCIAEARRQYKLFNVKNYGARSDGNTDNSVAFLKAWSDACRWNGKATVFIPEGTYMLKEVVFSGPCNAWMNFKIEGLLKAPSDPYSFKTDNWINFRYVNKLTVGGGGRLDGQGSSAWKINDCKKNSNCRSLPTTMKFDFITNGYVHHMESINSKQSHFVLFGCENMTLRRLKITAPNDSPNTDGIKIGRSNGINISSVNIGTGDDCIAMLSGTRNVRISDVFCGPGHGISVGSLGKNEGEEDIDDIVVKNCTFSGTSNGVRIKSWESQLKKTLVVSNFMYEDIVMDNVQYPIVIDQDYCPRPPCTKKGVSSVQISNVAYKNIRGSGNTKVAASFECSSNKPCQNIRVEDINLRPSGRSKRLNNVCLFVKGASYGQQNPPSCIK