AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene03618

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03618.t1 MTR_3g438050 92.405 395 30 0 1 395 1 395 0.0 746
MS.gene03618.t1 MTR_8g030930 60.401 399 153 4 1 395 1 398 1.73e-170 482
MS.gene03618.t1 MTR_5g063430 58.228 316 125 3 2 316 1 310 1.16e-132 383
MS.gene03618.t1 MTR_1g088160 40.741 405 218 9 7 395 8 406 2.08e-100 304
MS.gene03618.t1 MTR_2g058840 41.033 368 191 6 9 365 12 364 3.93e-94 288
MS.gene03618.t1 MTR_2g058670 41.033 368 193 7 9 365 12 366 1.56e-93 287
MS.gene03618.t1 MTR_2g435640 40.800 375 212 7 26 394 52 422 2.22e-92 284
MS.gene03618.t1 MTR_2g058640 39.510 367 200 6 9 365 12 366 3.79e-91 280
MS.gene03618.t1 MTR_4g083660 40.897 379 207 10 24 395 6 374 1.69e-90 278
MS.gene03618.t1 MTR_2g049400 39.846 389 224 7 9 395 11 391 3.16e-89 275
MS.gene03618.t1 MTR_5g097860 41.615 322 179 5 25 341 23 340 4.37e-85 263
MS.gene03618.t1 MTR_6g028080 40.947 359 186 9 27 372 30 375 1.96e-83 260
MS.gene03618.t1 MTR_8g009810 38.213 403 228 11 4 395 8 400 1.10e-82 258
MS.gene03618.t1 MTR_4g120320 38.298 376 220 7 26 394 51 421 9.40e-81 254
MS.gene03618.t1 MTR_8g065030 37.401 377 223 7 26 395 64 434 1.32e-80 255
MS.gene03618.t1 MTR_1g052315 40.642 374 209 8 27 394 64 430 1.54e-80 254
MS.gene03618.t1 MTR_4g084050 40.157 381 200 9 7 372 5 372 5.07e-80 251
MS.gene03618.t1 MTR_7g091850 38.501 387 225 8 13 391 60 441 9.81e-79 249
MS.gene03618.t1 MTR_3g030540 41.689 367 201 9 5 365 49 408 1.97e-78 248
MS.gene03618.t1 MTR_3g084380 39.096 376 204 9 11 372 1 365 2.75e-78 247
MS.gene03618.t1 MTR_2g103650 36.788 386 228 9 7 381 8 388 4.67e-78 246
MS.gene03618.t1 MTR_2g103410 35.904 376 229 6 27 395 59 429 1.20e-76 244
MS.gene03618.t1 MTR_3g054320 37.949 390 210 10 9 381 4 378 8.36e-76 241
MS.gene03618.t1 MTR_2g060350 40.771 363 195 10 17 370 68 419 2.36e-75 241
MS.gene03618.t1 MTR_2g029170 41.228 342 184 11 61 395 30 361 7.14e-75 237
MS.gene03618.t1 MTR_8g010070 37.240 384 218 10 5 381 7 374 5.57e-74 236
MS.gene03618.t1 MTR_1g086390 36.364 385 224 9 24 392 78 457 5.97e-74 238
MS.gene03618.t1 MTR_3g084360 38.873 355 198 7 27 372 24 368 5.98e-74 236
MS.gene03618.t1 MTR_8g010080 36.340 388 224 10 1 381 1 372 4.26e-73 233
MS.gene03618.t1 MTR_8g008765 36.944 360 198 8 1 349 1 342 1.11e-71 228
MS.gene03618.t1 MTR_8g010040 37.222 360 201 9 25 378 30 370 1.58e-71 229
MS.gene03618.t1 MTR_8g010110 36.915 363 203 9 26 381 30 373 4.64e-71 228
MS.gene03618.t1 MTR_7g110050 35.219 389 229 11 24 395 64 446 5.94e-71 230
MS.gene03618.t1 MTR_4g094715 35.476 389 223 9 26 395 64 443 6.37e-71 230
MS.gene03618.t1 MTR_2g100040 38.420 367 170 9 2 364 3 317 1.11e-70 226
MS.gene03618.t1 MTR_5g043350 38.674 362 200 8 7 355 5 357 1.33e-70 227
MS.gene03618.t1 MTR_6g005630 35.735 347 208 9 24 365 42 378 9.98e-70 226
MS.gene03618.t1 MTR_7g091840 39.375 320 182 7 25 336 63 378 3.23e-69 223
MS.gene03618.t1 MTR_8g010090 34.711 363 211 9 26 381 30 373 7.89e-68 219
MS.gene03618.t1 MTR_0157s0060 39.601 351 189 9 30 371 1 337 3.69e-66 214
MS.gene03618.t1 MTR_4g034020 39.601 351 189 9 30 371 1 337 3.69e-66 214
MS.gene03618.t1 MTR_7g035415 37.888 322 174 11 5 311 10 320 3.16e-64 209
MS.gene03618.t1 MTR_1g012390 35.250 400 239 14 7 395 5 395 5.84e-63 207
MS.gene03618.t1 MTR_5g033680 33.242 364 224 7 25 376 57 413 1.27e-61 206
MS.gene03618.t1 MTR_5g034090 31.937 382 224 8 24 374 118 494 1.98e-61 207
MS.gene03618.t1 MTR_8g065010 30.730 397 235 9 24 385 104 495 5.15e-60 203
MS.gene03618.t1 MTR_2g032710 33.419 389 231 9 26 395 66 445 5.86e-60 201
MS.gene03618.t1 MTR_8g077320 35.013 377 178 12 25 385 31 356 2.21e-59 197
MS.gene03618.t1 MTR_8g010050 33.073 384 200 10 5 381 7 340 9.87e-58 192
MS.gene03618.t1 MTR_7g113910 36.859 312 162 7 87 395 74 353 3.55e-56 189
MS.gene03618.t1 MTR_6g005630 32.853 347 195 10 24 365 42 355 1.23e-55 188
MS.gene03618.t1 MTR_1g013730 32.337 368 218 11 7 365 4 349 5.52e-55 186
MS.gene03618.t1 MTR_2g103410 36.496 274 166 4 27 293 59 331 6.79e-55 184
MS.gene03618.t1 MTR_1g013740 31.300 377 224 11 2 365 3 357 4.09e-54 184
MS.gene03618.t1 MTR_8g008700 36.101 277 153 6 86 356 14 272 1.15e-48 167
MS.gene03618.t1 MTR_5g034090 34.266 286 157 5 24 278 118 403 2.31e-48 169
MS.gene03618.t1 MTR_8g031180 32.542 295 162 7 87 373 15 280 2.10e-38 139
MS.gene03618.t1 MTR_7g072360 43.119 109 40 1 169 277 42 128 3.77e-22 91.7
MS.gene03618.t1 MTR_7g072370 57.377 61 26 0 222 282 1 61 4.60e-17 75.5
MS.gene03618.t1 MTR_2g042740 23.210 405 262 14 26 394 55 446 5.75e-17 82.8
MS.gene03618.t1 MTR_4g124980 23.833 407 257 14 26 394 73 464 2.47e-16 80.9
MS.gene03618.t1 MTR_4g120730 23.733 375 235 14 28 365 41 401 1.47e-12 69.3
MS.gene03618.t1 MTR_3g449590 25.113 442 256 20 6 392 12 433 1.79e-12 68.9
MS.gene03618.t1 MTR_7g088550 24.199 281 172 8 31 277 54 327 1.36e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03618.t1 AT2G15450 48.223 394 196 7 7 395 13 403 1.99e-118 350
MS.gene03618.t1 AT1G17150 45.918 392 209 3 5 395 12 401 3.36e-118 349
MS.gene03618.t1 AT2G15460 48.205 390 194 7 7 391 13 399 9.77e-117 345
MS.gene03618.t1 AT2G15470 47.970 394 197 7 7 395 13 403 9.87e-117 346
MS.gene03618.t1 AT2G33160 47.179 390 201 4 9 394 14 402 1.35e-116 354
MS.gene03618.t1 AT1G43080 46.684 392 200 7 10 395 15 403 6.40e-116 343
MS.gene03618.t1 AT2G40310 46.819 393 202 6 8 395 13 403 2.63e-115 342
MS.gene03618.t1 AT4G13760 45.547 393 207 6 8 395 13 403 5.21e-115 341
MS.gene03618.t1 AT1G43090 49.167 360 177 5 10 365 15 372 6.48e-115 342
MS.gene03618.t1 AT1G78400 46.092 371 198 2 26 395 34 403 5.68e-113 336
MS.gene03618.t1 AT2G26620 47.680 388 194 6 13 395 18 401 1.67e-112 335
MS.gene03618.t1 AT1G43100 48.333 360 180 5 10 365 15 372 1.06e-111 334
MS.gene03618.t1 AT3G07820 39.633 381 220 2 23 394 7 386 1.32e-100 304
MS.gene03618.t1 AT4G18180 40.674 386 210 7 26 394 38 421 1.79e-99 302
MS.gene03618.t1 AT3G07840 40.563 355 210 1 40 394 34 387 5.72e-99 300
MS.gene03618.t1 AT3G07830 37.150 393 241 2 2 394 1 387 3.29e-98 298
MS.gene03618.t1 AT1G05650 41.688 391 217 8 8 395 10 392 6.19e-98 297
MS.gene03618.t1 AT5G48140 41.127 355 207 2 40 394 34 386 7.59e-98 297
MS.gene03618.t1 AT1G05660 41.432 391 218 8 8 395 10 392 1.41e-97 296
MS.gene03618.t1 AT2G43880 41.129 372 208 7 27 395 31 394 2.25e-96 293
MS.gene03618.t1 AT2G43890 39.440 393 224 9 7 395 10 392 1.12e-94 289
MS.gene03618.t1 AT3G59850 43.733 375 196 10 27 395 23 388 3.71e-94 287
MS.gene03618.t1 AT3G59850 43.733 375 196 10 27 395 67 432 1.02e-93 288
MS.gene03618.t1 AT1G02790 39.947 378 210 6 26 394 51 420 2.14e-90 279
MS.gene03618.t1 AT2G43870 40.526 380 205 10 7 381 8 371 4.00e-90 277
MS.gene03618.t1 AT1G80170 38.095 399 229 8 10 395 31 424 1.59e-88 275
MS.gene03618.t1 AT3G14040 41.444 374 206 5 27 394 71 437 5.01e-87 271
MS.gene03618.t1 AT2G43880 44.966 298 156 5 27 321 31 323 6.17e-86 265
MS.gene03618.t1 AT3G57510 41.081 370 200 8 29 391 70 428 7.62e-86 268
MS.gene03618.t1 AT3G07850 41.471 340 188 4 61 394 102 436 1.01e-85 268
MS.gene03618.t1 AT3G07970 40.162 371 211 7 27 391 71 436 4.66e-81 255
MS.gene03618.t1 AT2G43860 40.730 356 198 8 16 365 22 370 8.56e-80 251
MS.gene03618.t1 AT2G41850 39.718 355 202 7 18 365 59 408 1.64e-79 251
MS.gene03618.t1 AT4G35670 39.237 367 203 9 27 385 24 378 3.60e-78 246
MS.gene03618.t1 AT1G65570 37.783 397 231 10 5 395 11 397 1.57e-77 245
MS.gene03618.t1 AT5G17200 37.440 414 217 8 7 389 13 415 7.74e-77 244
MS.gene03618.t1 AT1G70500 35.065 385 235 8 19 395 59 436 8.23e-76 243
MS.gene03618.t1 AT1G23460 35.714 378 230 7 25 395 41 412 4.90e-75 240
MS.gene03618.t1 AT1G23460 35.714 378 230 7 25 395 65 436 8.23e-75 240
MS.gene03618.t1 AT1G02460 35.467 375 218 9 26 381 88 457 3.13e-74 239
MS.gene03618.t1 AT3G15720 37.838 370 201 10 5 365 8 357 1.27e-73 237
MS.gene03618.t1 AT3G15720 37.838 370 201 10 5 365 7 356 1.29e-73 237
MS.gene03618.t1 AT3G15720 37.838 370 198 10 5 365 7 353 3.28e-72 233
MS.gene03618.t1 AT5G44840 36.702 376 215 11 7 374 9 369 9.99e-71 227
MS.gene03618.t1 AT1G48100 34.536 388 228 7 26 395 79 458 1.83e-69 226
MS.gene03618.t1 AT5G14650 36.579 380 224 12 24 395 48 418 6.24e-69 224
MS.gene03618.t1 AT3G26610 35.714 378 226 8 26 392 66 437 1.57e-68 224
MS.gene03618.t1 AT1G56710 35.233 386 229 8 26 395 47 427 2.91e-67 219
MS.gene03618.t1 AT5G27530 37.079 356 195 10 26 372 52 387 1.56e-65 216
MS.gene03618.t1 AT4G01890 33.067 375 227 9 26 381 64 433 2.02e-65 216
MS.gene03618.t1 AT1G10640 32.836 402 229 10 24 393 116 508 4.83e-64 214
MS.gene03618.t1 AT1G80170 37.710 297 170 6 10 294 31 324 6.14e-62 202
MS.gene03618.t1 AT1G80140 37.209 344 191 10 64 395 6 336 2.80e-61 201
MS.gene03618.t1 AT1G60590 32.267 375 215 8 27 367 123 492 1.93e-60 204
MS.gene03618.t1 AT3G15720 38.562 306 166 8 68 365 2 293 7.84e-60 199
MS.gene03618.t1 AT4G32380 33.609 363 223 7 41 394 41 394 1.02e-59 200
MS.gene03618.t1 AT5G44830 36.778 329 180 10 54 374 2 310 4.99e-59 195
MS.gene03618.t1 AT4G32375 35.764 288 173 4 100 385 8 285 5.45e-57 192
MS.gene03618.t1 AT5G39910 36.770 291 155 6 25 306 34 304 1.10e-56 190
MS.gene03618.t1 AT4G35670 38.710 279 158 5 105 382 2 268 3.38e-55 184
MS.gene03618.t1 AT4G32370 37.847 288 156 11 41 315 49 326 4.45e-52 177
MS.gene03618.t1 AT5G14650 39.080 261 149 7 24 278 48 304 1.45e-50 172
MS.gene03618.t1 AT4G32380 34.000 300 181 6 41 333 41 330 1.30e-46 165
MS.gene03618.t1 AT5G41870 23.448 435 273 14 7 394 11 432 2.50e-17 84.0
MS.gene03618.t1 AT3G42950 24.324 407 255 18 26 394 65 456 2.06e-15 78.2
MS.gene03618.t1 AT1G19170 23.358 411 255 14 26 394 86 478 3.53e-15 77.4
MS.gene03618.t1 AT3G48950 23.043 447 274 19 5 395 6 438 3.31e-13 71.2
MS.gene03618.t1 AT2G23900 25.352 284 166 11 31 277 60 334 3.69e-13 71.2
MS.gene03618.t1 AT4G23820 25.526 380 223 17 27 365 41 401 4.29e-13 70.9
MS.gene03618.t1 AT3G62110 24.054 370 240 13 28 365 44 404 4.36e-13 70.9
MS.gene03618.t1 AT3G62110 24.054 370 240 13 28 365 44 404 4.36e-13 70.9

Find 66 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGCTGATGTCTCTTCTTT+TGG 0.169444 3.4:-33732484 MS.gene03618:CDS
TTCACCATATATGTTCCTTT+TGG 0.225532 3.4:+33734622 None:intergenic
ATTGGAAGACGGGGACAATT+AGG 0.285173 3.4:+33734400 None:intergenic
CATTTATCAGTCGCAAAATA+TGG 0.292416 3.4:+33734523 None:intergenic
GATCCCAAAAGGAACATATA+TGG 0.319723 3.4:-33734626 MS.gene03618:CDS
TTATTGCTGCAACCACGATT+TGG 0.338462 3.4:+33733114 None:intergenic
GATTGTATCGCGATGGTATC+TGG 0.369935 3.4:-33733399 MS.gene03618:CDS
GGAAGTCTTGGAAAGTTTGA+TGG 0.376257 3.4:-33733318 MS.gene03618:CDS
TCCCCTCACTAAAACTTTGA+AGG 0.385919 3.4:-33733211 MS.gene03618:CDS
CAAAATTGAAGGGACCTTGA+AGG 0.390336 3.4:-33734557 MS.gene03618:CDS
TTGGAAGACGGGGACAATTA+GGG 0.408182 3.4:+33734401 None:intergenic
TATCTGGCACTAGGAATGTT+TGG 0.416521 3.4:-33733383 MS.gene03618:CDS
TGCTATTAAAAGATCTCAAA+TGG 0.416555 3.4:+33733574 None:intergenic
CATCATTAGGCCACAAATCA+AGG 0.424004 3.4:+33732536 None:intergenic
GTGAAGCAAGTAATCTTCAT+TGG 0.433473 3.4:-33734604 MS.gene03618:CDS
GGAGTTAGTATTGGAAGTCT+TGG 0.436140 3.4:-33733330 MS.gene03618:CDS
AAATCGTGGTTGCAGCAATA+AGG 0.436370 3.4:-33733112 MS.gene03618:intron
GTAAAAGAAAACTAAACAAC+TGG 0.440115 3.4:-33732513 MS.gene03618:CDS
TCTGTGTTTGGGCTATCACC+CGG 0.444875 3.4:+33733486 None:intergenic
TGGATCAGTTGGAGCCTTCA+AGG 0.450279 3.4:+33734543 None:intergenic
AATTTGATCGTGAGTGGACC+TGG 0.453283 3.4:-33734478 MS.gene03618:CDS
CTGTGTTTGGGCTATCACCC+GGG 0.456362 3.4:+33733487 None:intergenic
ATTGGATGGTCAAGGATCTT+CGG 0.463981 3.4:-33734452 MS.gene03618:CDS
TATTCTTGATCAATAAGAAT+GGG 0.473017 3.4:+33733141 None:intergenic
CGTGAGTGGACCTGGGACAT+TGG 0.479525 3.4:-33734470 MS.gene03618:CDS
TGGACAGCTTTCAAAATTGA+AGG 0.481535 3.4:-33734568 MS.gene03618:CDS
ACCTTTAGTGACACAACTAA+TGG 0.486200 3.4:-33733258 MS.gene03618:CDS
CCTGGACATGGAGTTAGTAT+TGG 0.492823 3.4:-33733339 MS.gene03618:CDS
AAAGCTGTCCATCCTTTGCA+TGG 0.498912 3.4:+33734580 None:intergenic
CAGTGGAAAACCTTGATTTG+TGG 0.503671 3.4:-33732546 MS.gene03618:CDS
AGTGATGCATGCAAATCAAA+TGG 0.505240 3.4:-33734664 MS.gene03618:CDS
TGAACTTACAATTGGAAGAC+GGG 0.511895 3.4:+33734390 None:intergenic
AAAAGATCTCAAATGGTGAA+TGG 0.512142 3.4:+33733581 None:intergenic
GTCGCAAAATATGGATCAGT+TGG 0.512826 3.4:+33734532 None:intergenic
ATATTCTTGATCAATAAGAA+TGG 0.516058 3.4:+33733140 None:intergenic
CTTGATTTGTGGCCTAATGA+TGG 0.522046 3.4:-33732535 MS.gene03618:CDS
ATGGTCTTAGAATTAAGACT+TGG 0.524419 3.4:-33733239 MS.gene03618:CDS
ACCATTAGTTGTGTCACTAA+AGG 0.526950 3.4:+33733257 None:intergenic
ATGGTCAAGGATCTTCGGCT+TGG 0.534419 3.4:-33734447 MS.gene03618:CDS
AACTACGGTGCAATCGCAGA+TGG 0.556221 3.4:-33734821 MS.gene03618:CDS
GTGAATAATTTGATCGTGAG+TGG 0.559550 3.4:-33734484 MS.gene03618:CDS
TGTGACAAAATCAAATGTCA+TGG 0.564891 3.4:+33733611 None:intergenic
TGGAGAAACTGATAATAAAG+AGG 0.567699 3.4:-33734801 MS.gene03618:intron
TCATTGGACCATGCAAAGGA+TGG 0.568404 3.4:-33734588 MS.gene03618:CDS
AAAATCAGAATTTCAGCCCC+GGG 0.568624 3.4:-33733504 MS.gene03618:CDS
TGGTCTTAGAATTAAGACTT+GGG 0.575173 3.4:-33733238 MS.gene03618:CDS
CCTTGACCATCCAATGTCCC+AGG 0.577038 3.4:+33734460 None:intergenic
TCAAGGATCTTCGGCTTGGT+CGG 0.577538 3.4:-33734443 MS.gene03618:CDS
GGTGATAGCCCAAACACAGA+TGG 0.580283 3.4:-33733483 MS.gene03618:CDS
ATCTTCATTGGACCATGCAA+AGG 0.586443 3.4:-33734592 MS.gene03618:CDS
TCGCGATGGTATCTGGCACT+AGG 0.592055 3.4:-33733392 MS.gene03618:CDS
AGTGGACCTGGGACATTGGA+TGG 0.596328 3.4:-33734466 MS.gene03618:CDS
ATGAACTTACAATTGGAAGA+CGG 0.597247 3.4:+33734389 None:intergenic
CCTGGGACATTGGATGGTCA+AGG 0.604515 3.4:-33734460 MS.gene03618:CDS
CCAATACTAACTCCATGTCC+AGG 0.607391 3.4:+33733339 None:intergenic
GGACAGCTTTCAAAATTGAA+GGG 0.612137 3.4:-33734567 MS.gene03618:CDS
CGGTGATGATTGTATCGCGA+TGG 0.625011 3.4:-33733406 MS.gene03618:CDS
CAAGAATATTGTCCAAATCG+TGG 0.629259 3.4:-33733126 MS.gene03618:CDS
TGTGTTTGGGCTATCACCCG+GGG 0.645369 3.4:+33733488 None:intergenic
ATTTGATCGTGAGTGGACCT+GGG 0.645399 3.4:-33734477 MS.gene03618:CDS
CATCGCGATACAATCATCAC+CGG 0.662603 3.4:+33733407 None:intergenic
TAACACTATAGGCACGATGG+AGG 0.665555 3.4:+33732451 None:intergenic
ATTTAACACTATAGGCACGA+TGG 0.672686 3.4:+33732448 None:intergenic
AGATATTGTGATGAATAACG+TGG 0.711571 3.4:-33733169 MS.gene03618:CDS
GAACTTACAATTGGAAGACG+GGG 0.712367 3.4:+33734391 None:intergenic
GCCATGCCAAAATATCACAG+TGG 0.766589 3.4:-33732563 MS.gene03618:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTTAATATTAAAATT+AGG + chr3.4:33734301-33734320 None:intergenic 0.0%
!!! AATATGATTTATCTTTTTAA+CGG - chr3.4:33733633-33733652 MS.gene03618:intron 10.0%
!!! GTAGAATATAATTTTTTTTA+AGG + chr3.4:33733029-33733048 None:intergenic 10.0%
!! AAATGTAACATTTAACATAT+TGG + chr3.4:33733666-33733685 None:intergenic 15.0%
!! GTTAAAAAGATAAATCATAT+TGG + chr3.4:33733634-33733653 None:intergenic 15.0%
!! TAGAAATAAAAACTAAAATG+TGG + chr3.4:33733471-33733490 None:intergenic 15.0%
!! TTTAAGAAATAAAAAAGAGA+AGG + chr3.4:33732616-33732635 None:intergenic 15.0%
!!! ATATGATTTATCTTTTTAAC+GGG - chr3.4:33733634-33733653 MS.gene03618:intron 15.0%
!!! ATTTTTAAGATACCTAATTT+TGG - chr3.4:33734577-33734596 MS.gene03618:CDS 15.0%
!!! ATTTTTTTTAAGGATATCTA+TGG + chr3.4:33733019-33733038 None:intergenic 15.0%
!!! TTCATGATTTATTTTCATAT+TGG + chr3.4:33733397-33733416 None:intergenic 15.0%
!!! TTTTTAAGATACCTAATTTT+GGG - chr3.4:33734578-33734597 MS.gene03618:CDS 15.0%
!! AAGAGAATAACAAATTAGTT+AGG + chr3.4:33732655-33732674 None:intergenic 20.0%
!! ATAAAATATCTCCCAAAATT+AGG + chr3.4:33734592-33734611 None:intergenic 20.0%
!! ATATTCTTGATCAATAAGAA+TGG + chr3.4:33734230-33734249 None:intergenic 20.0%
!! TAATTGAAGCATATATTCTA+TGG - chr3.4:33733583-33733602 MS.gene03618:CDS 20.0%
!! TATTCTTGATCAATAAGAAT+GGG + chr3.4:33734229-33734248 None:intergenic 20.0%
!! TGTTATTCTCTTGTAAATAT+AGG - chr3.4:33732663-33732682 MS.gene03618:CDS 20.0%
!! TTAACATGATATTAATGCAT+TGG - chr3.4:33733714-33733733 MS.gene03618:intron 20.0%
!! TTAATAGATGAACTTACAAT+TGG + chr3.4:33732988-33733007 None:intergenic 20.0%
!!! AAAGGTAAAATTTTGATTTG+AGG - chr3.4:33734545-33734564 MS.gene03618:CDS 20.0%
!!! GTTTTTAATGTGTTAAACTA+CGG - chr3.4:33732531-33732550 MS.gene03618:CDS 20.0%
!!! TAGTTTTAAATTCCTTTGTT+AGG - chr3.4:33733214-33733233 MS.gene03618:CDS 20.0%
!!! TTTAGCAAATTTGATTTAGT+CGG - chr3.4:33734390-33734409 MS.gene03618:intron 20.0%
!!! TTTTTGAAAGATAAAGTGAA+GGG - chr3.4:33733160-33733179 MS.gene03618:CDS 20.0%
!!! TTTTTTGAAAGATAAAGTGA+AGG - chr3.4:33733159-33733178 MS.gene03618:CDS 20.0%
!!! TTTTTTTGTGTACATTTAGT+CGG - chr3.4:33734424-33734443 MS.gene03618:CDS 20.0%
! AAAGAAATACAAACTCTCTA+CGG + chr3.4:33734496-33734515 None:intergenic 25.0%
! AAAGATAAATCATATTGGTC+TGG + chr3.4:33733629-33733648 None:intergenic 25.0%
! AACACAGATGGAATAAAAAT+AGG - chr3.4:33733896-33733915 MS.gene03618:intron 25.0%
! AATAATAATCCCTTCACTTT+AGG + chr3.4:33733132-33733151 None:intergenic 25.0%
! AATTGGCAGAAATTAGATTA+TGG + chr3.4:33734673-33734692 None:intergenic 25.0%
! ATAATAATCCCTTCACTTTA+GGG + chr3.4:33733131-33733150 None:intergenic 25.0%
! GTAAAAGAAAACTAAACAAC+TGG - chr3.4:33734854-33734873 MS.gene03618:CDS 25.0%
! TATTCCAATTTATTTCCTCT+TGG + chr3.4:33733094-33733113 None:intergenic 25.0%
! TCTAGTGATAAATTTGTCAT+AGG - chr3.4:33733343-33733362 MS.gene03618:CDS 25.0%
! TGCTATTAAAAGATCTCAAA+TGG + chr3.4:33733796-33733815 None:intergenic 25.0%
! TGTGTTGGATATAAGTTAAA+AGG - chr3.4:33734527-33734546 MS.gene03618:CDS 25.0%
! TTAAATTCCTTTGTTAGGAT+TGG - chr3.4:33733219-33733238 MS.gene03618:CDS 25.0%
! TTAACCAAGAGGAAATAAAT+TGG - chr3.4:33733087-33733106 MS.gene03618:intron 25.0%
!! AGTTTTCTTTTACCATCATT+AGG + chr3.4:33734847-33734866 None:intergenic 25.0%
!! ATTTTTATTCCATCTGTGTT+TGG + chr3.4:33733896-33733915 None:intergenic 25.0%
!! CTTTTTCTTCTTTATTGTGT+TGG - chr3.4:33734512-33734531 MS.gene03618:CDS 25.0%
!! TTGAGATCTTTTAATAGCAA+AGG - chr3.4:33733797-33733816 MS.gene03618:intron 25.0%
!! TTTTTATTCCATCTGTGTTT+GGG + chr3.4:33733895-33733914 None:intergenic 25.0%
!!! ACATTTGATTTTGTCACAAA+TGG - chr3.4:33733761-33733780 MS.gene03618:intron 25.0%
!!! GTATCATGATTTTGATTTGA+TGG + chr3.4:33733371-33733390 None:intergenic 25.0%
!!! TACATATGTTTTTAACCAAG+AGG - chr3.4:33733076-33733095 MS.gene03618:intron 25.0%
AAAAGATCTCAAATGGTGAA+TGG + chr3.4:33733789-33733808 None:intergenic 30.0%
AAACAAAAAAGAACAACCAC+GGG + chr3.4:33733448-33733467 None:intergenic 30.0%
ACTATTAAAAACGCGAACAT+TGG - chr3.4:33733935-33733954 MS.gene03618:intron 30.0%
AGATATTGTGATGAATAACG+TGG - chr3.4:33734198-33734217 MS.gene03618:intron 30.0%
ATATTCTACAGATCGATCAA+TGG - chr3.4:33733040-33733059 MS.gene03618:intron 30.0%
ATGAACTTACAATTGGAAGA+CGG + chr3.4:33732981-33733000 None:intergenic 30.0%
CATTTATCAGTCGCAAAATA+TGG + chr3.4:33732847-33732866 None:intergenic 30.0%
GAAACAAAAAAGAACAACCA+CGG + chr3.4:33733449-33733468 None:intergenic 30.0%
GAGGAAATAAATTGGAATAG+CGG - chr3.4:33733095-33733114 MS.gene03618:intron 30.0%
GTGTCTTACAAAAATATCAG+AGG - chr3.4:33734733-33734752 MS.gene03618:intron 30.0%
TGGAGAAACTGATAATAAAG+AGG - chr3.4:33732566-33732585 MS.gene03618:CDS 30.0%
TGTGACAAAATCAAATGTCA+TGG + chr3.4:33733759-33733778 None:intergenic 30.0%
TTAGGATTGGAGGAATAATT+GGG - chr3.4:33733232-33733251 MS.gene03618:CDS 30.0%
TTATAACAAAATGTGATCGC+TGG + chr3.4:33733320-33733339 None:intergenic 30.0%
! ATGGTCTTAGAATTAAGACT+TGG - chr3.4:33734128-33734147 MS.gene03618:intron 30.0%
! CATATTTTGCGACTGATAAA+TGG - chr3.4:33732845-33732864 MS.gene03618:intron 30.0%
! GCATATATTCTATGGATTGA+TGG - chr3.4:33733591-33733610 MS.gene03618:CDS 30.0%
! TCACCATATATGTTCCTTTT+GGG + chr3.4:33732747-33732766 None:intergenic 30.0%
! TCGCGTTTTTAATAGTTATC+TGG + chr3.4:33733931-33733950 None:intergenic 30.0%
! TGGTCTTAGAATTAAGACTT+GGG - chr3.4:33734129-33734148 MS.gene03618:intron 30.0%
! TTCACCATATATGTTCCTTT+TGG + chr3.4:33732748-33732767 None:intergenic 30.0%
AAAAAGAGAAGGAGTGAAGA+TGG + chr3.4:33732605-33732624 None:intergenic 35.0%
AAAAATCAGAATTTCAGCCC+CGG - chr3.4:33733862-33733881 MS.gene03618:intron 35.0%
AATTCCTTTGTTAGGATTGG+AGG - chr3.4:33733222-33733241 MS.gene03618:CDS 35.0%
ACCTTTAGTGACACAACTAA+TGG - chr3.4:33734109-33734128 MS.gene03618:intron 35.0%
AGTGATGCATGCAAATCAAA+TGG - chr3.4:33732703-33732722 MS.gene03618:intron 35.0%
CAAGAATATTGTCCAAATCG+TGG - chr3.4:33734241-33734260 MS.gene03618:intron 35.0%
CCACCTACAGCAAAATTAAT+TGG + chr3.4:33734690-33734709 None:intergenic 35.0%
GATCCCAAAAGGAACATATA+TGG - chr3.4:33732741-33732760 MS.gene03618:intron 35.0%
GGACAGCTTTCAAAATTGAA+GGG - chr3.4:33732800-33732819 MS.gene03618:intron 35.0%
GTGAAGCAAGTAATCTTCAT+TGG - chr3.4:33732763-33732782 MS.gene03618:intron 35.0%
GTGAATAATTTGATCGTGAG+TGG - chr3.4:33732883-33732902 MS.gene03618:intron 35.0%
GTTAGGATTGGAGGAATAAT+TGG - chr3.4:33733231-33733250 MS.gene03618:CDS 35.0%
TATTCCTCCAATCCTAACAA+AGG + chr3.4:33733229-33733248 None:intergenic 35.0%
TGAACTTACAATTGGAAGAC+GGG + chr3.4:33732980-33732999 None:intergenic 35.0%
TGGACAGCTTTCAAAATTGA+AGG - chr3.4:33732799-33732818 MS.gene03618:intron 35.0%
! ACCATTAGTTGTGTCACTAA+AGG + chr3.4:33734113-33734132 None:intergenic 35.0%
! GGTTTTCCACTGTGATATTT+TGG + chr3.4:33734813-33734832 None:intergenic 35.0%
! TATAGGCGTTTTTGAATGCA+TGG - chr3.4:33732680-33732699 MS.gene03618:intron 35.0%
!! AAGCCTTCAAAGTTTTAGTG+AGG + chr3.4:33734162-33734181 None:intergenic 35.0%
!! AGCCTTCAAAGTTTTAGTGA+GGG + chr3.4:33734161-33734180 None:intergenic 35.0%
!! CCAATTAATTTTGCTGTAGG+TGG - chr3.4:33734687-33734706 MS.gene03618:intron 35.0%
!! CTGCCAATTAATTTTGCTGT+AGG - chr3.4:33734684-33734703 MS.gene03618:CDS 35.0%
!!! GTCACTATTTTGATCCCAAA+AGG - chr3.4:33732730-33732749 MS.gene03618:intron 35.0%
!!! TGGATCTCTGATGTTTTTTG+TGG - chr3.4:33734004-33734023 MS.gene03618:intron 35.0%
AAAATCAGAATTTCAGCCCC+GGG - chr3.4:33733863-33733882 MS.gene03618:intron 40.0%
AAATCGTGGTTGCAGCAATA+AGG - chr3.4:33734255-33734274 MS.gene03618:intron 40.0%
AACAACCACCCTAAAGTGAA+GGG - chr3.4:33733120-33733139 MS.gene03618:CDS 40.0%
AGAAGGAGTGAAGATGGTTA+TGG + chr3.4:33732599-33732618 None:intergenic 40.0%
ATAGAAGAAAAACAGTCCCG+TGG - chr3.4:33733429-33733448 MS.gene03618:CDS 40.0%
ATCTTCATTGGACCATGCAA+AGG - chr3.4:33732775-33732794 MS.gene03618:intron 40.0%
ATTGGATGGTCAAGGATCTT+CGG - chr3.4:33732915-33732934 MS.gene03618:intron 40.0%
CAAAATTGAAGGGACCTTGA+AGG - chr3.4:33732810-33732829 MS.gene03618:intron 40.0%
CATCATTAGGCCACAAATCA+AGG + chr3.4:33734834-33734853 None:intergenic 40.0%
GAACTTACAATTGGAAGACG+GGG + chr3.4:33732979-33732998 None:intergenic 40.0%
GGAAGTCTTGGAAAGTTTGA+TGG - chr3.4:33734049-33734068 MS.gene03618:intron 40.0%
GTCGCAAAATATGGATCAGT+TGG + chr3.4:33732838-33732857 None:intergenic 40.0%
TAACAACCACCCTAAAGTGA+AGG - chr3.4:33733119-33733138 MS.gene03618:CDS 40.0%
TATCTGGCACTAGGAATGTT+TGG - chr3.4:33733984-33734003 MS.gene03618:intron 40.0%
TCCCCTCACTAAAACTTTGA+AGG - chr3.4:33734156-33734175 MS.gene03618:intron 40.0%
TTATTGCTGCAACCACGATT+TGG + chr3.4:33734256-33734275 None:intergenic 40.0%
! ATAATCCCTTCACTTTAGGG+TGG + chr3.4:33733128-33733147 None:intergenic 40.0%
! CAGTGGAAAACCTTGATTTG+TGG - chr3.4:33734821-33734840 MS.gene03618:CDS 40.0%
! CATGCTGATGTCTCTTCTTT+TGG - chr3.4:33734883-33734902 MS.gene03618:CDS 40.0%
! GGAGTTAGTATTGGAAGTCT+TGG - chr3.4:33734037-33734056 MS.gene03618:intron 40.0%
!! CTTGATTTGTGGCCTAATGA+TGG - chr3.4:33734832-33734851 MS.gene03618:CDS 40.0%
!! GCCTTCAAAGTTTTAGTGAG+GGG + chr3.4:33734160-33734179 None:intergenic 40.0%
!! GCTCTTTTTCACTGCATTTC+AGG + chr3.4:33734786-33734805 None:intergenic 40.0%
!!! TCCACTGTGATATTTTGGCA+TGG + chr3.4:33734808-33734827 None:intergenic 40.0%
!!! TCTGATGTTTTTTGTGGTCC+TGG - chr3.4:33734010-33734029 MS.gene03618:intron 40.0%
AAAAACGCGAACATTGGCAC+CGG - chr3.4:33733941-33733960 MS.gene03618:intron 45.0%
AAAGCTGTCCATCCTTTGCA+TGG + chr3.4:33732790-33732809 None:intergenic 45.0%
AATTTGATCGTGAGTGGACC+TGG - chr3.4:33732889-33732908 MS.gene03618:intron 45.0%
ATTGGAAGACGGGGACAATT+AGG + chr3.4:33732970-33732989 None:intergenic 45.0%
ATTTGATCGTGAGTGGACCT+GGG - chr3.4:33732890-33732909 MS.gene03618:intron 45.0%
CATCGCGATACAATCATCAC+CGG + chr3.4:33733963-33733982 None:intergenic 45.0%
CCAATACTAACTCCATGTCC+AGG + chr3.4:33734031-33734050 None:intergenic 45.0%
CCTGGACATGGAGTTAGTAT+TGG - chr3.4:33734028-33734047 MS.gene03618:intron 45.0%
GATTGTATCGCGATGGTATC+TGG - chr3.4:33733968-33733987 MS.gene03618:intron 45.0%
GCCATGCCAAAATATCACAG+TGG - chr3.4:33734804-33734823 MS.gene03618:CDS 45.0%
TCATTGGACCATGCAAAGGA+TGG - chr3.4:33732779-33732798 MS.gene03618:intron 45.0%
TTGGAAGACGGGGACAATTA+GGG + chr3.4:33732969-33732988 None:intergenic 45.0%
!!! GTTTTTTGTGGTCCTGGACA+TGG - chr3.4:33734016-33734035 MS.gene03618:intron 45.0%
AACTACGGTGCAATCGCAGA+TGG - chr3.4:33732546-33732565 MS.gene03618:CDS 50.0%
ATGGTCAAGGATCTTCGGCT+TGG - chr3.4:33732920-33732939 MS.gene03618:intron 50.0%
CGGTGATGATTGTATCGCGA+TGG - chr3.4:33733961-33733980 MS.gene03618:intron 50.0%
GGTGATAGCCCAAACACAGA+TGG - chr3.4:33733884-33733903 MS.gene03618:intron 50.0%
TCTGTGTTTGGGCTATCACC+CGG + chr3.4:33733884-33733903 None:intergenic 50.0%
TGGATCAGTTGGAGCCTTCA+AGG + chr3.4:33732827-33732846 None:intergenic 50.0%
!! TCAAGGATCTTCGGCTTGGT+CGG - chr3.4:33732924-33732943 MS.gene03618:intron 50.0%
AGTGGACCTGGGACATTGGA+TGG - chr3.4:33732901-33732920 MS.gene03618:intron 55.0%
CCTGGGACATTGGATGGTCA+AGG - chr3.4:33732907-33732926 MS.gene03618:intron 55.0%
CCTTGACCATCCAATGTCCC+AGG + chr3.4:33732910-33732929 None:intergenic 55.0%
CTGTGTTTGGGCTATCACCC+GGG + chr3.4:33733883-33733902 None:intergenic 55.0%
TCGCGATGGTATCTGGCACT+AGG - chr3.4:33733975-33733994 MS.gene03618:intron 55.0%
TGTGTTTGGGCTATCACCCG+GGG + chr3.4:33733882-33733901 None:intergenic 55.0%
CGTGAGTGGACCTGGGACAT+TGG - chr3.4:33732897-33732916 MS.gene03618:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 33732456 33734933 33732456 ID=MS.gene03618
chr3.4 mRNA 33732456 33734933 33732456 ID=MS.gene03618.t1;Parent=MS.gene03618
chr3.4 exon 33734802 33734933 33734802 ID=MS.gene03618.t1.exon1;Parent=MS.gene03618.t1
chr3.4 CDS 33734802 33734933 33734802 ID=cds.MS.gene03618.t1;Parent=MS.gene03618.t1
chr3.4 exon 33734399 33734704 33734399 ID=MS.gene03618.t1.exon2;Parent=MS.gene03618.t1
chr3.4 CDS 33734399 33734704 33734399 ID=cds.MS.gene03618.t1;Parent=MS.gene03618.t1
chr3.4 exon 33733113 33733634 33733113 ID=MS.gene03618.t1.exon3;Parent=MS.gene03618.t1
chr3.4 CDS 33733113 33733634 33733113 ID=cds.MS.gene03618.t1;Parent=MS.gene03618.t1
chr3.4 exon 33732456 33732683 33732456 ID=MS.gene03618.t1.exon4;Parent=MS.gene03618.t1
chr3.4 CDS 33732456 33732683 33732456 ID=cds.MS.gene03618.t1;Parent=MS.gene03618.t1
Gene Sequence

>MS.gene03618

ATGGTTGGAAATTTTTCGTTTGTGTTGTTCTTACTAGTAGTTTGTATTTCAAAATCACAATCACGAGTCACAAACGTTTTTAATGTGTTAAACTACGGTGCAATCGCAGATGGAGAAACTGATAATAAAGAGGTATATAACCATAACCATCTTCACTCCTTCTCTTTTTTATTTCTTAAAATTTTGATTTAGATTACCTAACTAATTTGTTATTCTCTTGTAAATATAGGCGTTTTTGAATGCATGGAGTGATGCATGCAAATCAAATGGAAAAGTCACTATTTTGATCCCAAAAGGAACATATATGGTGAAGCAAGTAATCTTCATTGGACCATGCAAAGGATGGACAGCTTTCAAAATTGAAGGGACCTTGAAGGCTCCAACTGATCCATATTTTGCGACTGATAAATGGATTAATTTTCAATACGTGAATAATTTGATCGTGAGTGGACCTGGGACATTGGATGGTCAAGGATCTTCGGCTTGGTCGGTAAATGATTGCAAAAACAACCCTAATTGTCCCCGTCTTCCAATTGTAAGTTCATCTATTAAATCATATTCCATAGATATCCTTAAAAAAAATTATATTCTACAGATCGATCAATGGATTTTAACAAAATTACATATGTTTTTAACCAAGAGGAAATAAATTGGAATAGCGGATAACAACCACCCTAAAGTGAAGGGATTATTATTATTTTTTTTTTTTGAAAGATAAAGTGAAGGGATTATGTTACTATTCATTAATAATAGAAAAGTAGTTTTAAATTCCTTTGTTAGGATTGGAGGAATAATTGGGAATTTAGTTTCTATACTATCGTGTCTTATATATGTTTCAATAGTATCATGTTTAAATAGTTTCCAGCGATCACATTTTGTTATAATGTTCTAGTGATAAATTTGTCATAGGTACCATCAAATCAAAATCATGATACATACCAATATGAAAATAAATCATGAAAAAAAATCATCAATAGAAGAAAAACAGTCCCGTGGTTGTTCTTTTTTGTTTCCACATTTTAGTTTTTATTTCTATTTATAGCGTGTGTGTATATATATAATATATGTTAAGAGTTTTTTTTTTTTTTTGAATAAATATATTTAAGAGTTATTAGTAGTTATTTTTTTAATTGAAGCATATATTCTATGGATTGATGGAACAAATTGTAGCCAGACCAATATGATTTATCTTTTTAACGGGAGTCTACCAATATGTTAAATGTTACATTTTTTTATTATACTAGTTATTAATTTTTGTTTAACATGATATTAATGCATTGGTGTTTTTTGTTTTCTCAGTCCATGACATTTGATTTTGTCACAAATGGTACCATTCACCATTTGAGATCTTTTAATAGCAAAGGAGCACATTTTAAGTTGTTTGCTTGCGAAAACATGATTTTCACAAAAATCAGAATTTCAGCCCCGGGTGATAGCCCAAACACAGATGGAATAAAAATAGGCAAGTCAAACCAGATAACTATTAAAAACGCGAACATTGGCACCGGTGATGATTGTATCGCGATGGTATCTGGCACTAGGAATGTTTGGATCTCTGATGTTTTTTGTGGTCCTGGACATGGAGTTAGTATTGGAAGTCTTGGAAAGTTTGATGGAGAAGAAGATGTTGACAACATAAATGTGAAGAACTGCACCTTTAGTGACACAACTAATGGTCTTAGAATTAAGACTTGGGCTTCTCCCCTCACTAAAACTTTGAAGGCTTCTCATATTGTGTATGAAGATATTGTGATGAATAACGTGGATAATCCCATTCTTATTGATCAAGAATATTGTCCAAATCGTGGTTGCAGCAATAAGGTTAATACTTTTAAACTACATCCTAATTTTAATATTAAATTTAAATCACTTTTTTTCTCACTTTATTTTTTGTTAATTTGAATTTTTAATATTCATGCAATTTGAGATGTATTTTTAGCAAATTTGATTTAGTCGGAATAATTTTTTTTTTTTTGTGTACATTTAGTCGGAATATGAGTCGTCAAATTTTCATTCAAACTATGATAAAAAAAATTACCGTAGAGAGTTTGTATTTCTTTTTCTTCTTTATTGTGTTGGATATAAGTTAAAAGGTAAAATTTTGATTTGAGGTTTACTAATATTTTTAAGATACCTAATTTTGGGAGATATTTTATAAGTATTGATTGAAAATATAGATTAAATACTGATAATTGATATGACTTTACACATTTACCATAATCTAATTTCTGCCAATTAATTTTGCTGTAGGTGGCATCAAACGTGCAAATAAATACTGTGTCTTACAAAAATATCAGAGGAAGTGTGAATTCTCAACTTGCAATGAACCTGAAATGCAGTGAAAAAGAGCCATGCCAAAATATCACAGTGGAAAACCTTGATTTGTGGCCTAATGATGGTAAAAGAAAACTAAACAACTGGTGTTCACATGCTGATGTCTCTTCTTTTGGAAAACAAAATCCTCCATCGTGCCTATAG
Protein sequence

>MS.gene03618.t1

MVGNFSFVLFLLVVCISKSQSRVTNVFNVLNYGAIADGETDNKEAFLNAWSDACKSNGKVTILIPKGTYMVKQVIFIGPCKGWTAFKIEGTLKAPTDPYFATDKWINFQYVNNLIVSGPGTLDGQGSSAWSVNDCKNNPNCPRLPISMTFDFVTNGTIHHLRSFNSKGAHFKLFACENMIFTKIRISAPGDSPNTDGIKIGKSNQITIKNANIGTGDDCIAMVSGTRNVWISDVFCGPGHGVSIGSLGKFDGEEDVDNINVKNCTFSDTTNGLRIKTWASPLTKTLKASHIVYEDIVMNNVDNPILIDQEYCPNRGCSNKVASNVQINTVSYKNIRGSVNSQLAMNLKCSEKEPCQNITVENLDLWPNDGKRKLNNWCSHADVSSFGKQNPPSCL