AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02144

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02144.t1 MTR_2g031370 98.734 237 3 0 1 237 13 249 1.31e-176 485
MS.gene02144.t1 MTR_2g031370 98.734 237 3 0 1 237 1 237 2.22e-176 484
MS.gene02144.t1 MTR_4g094525 69.068 236 72 1 1 235 1 236 1.78e-114 327
MS.gene02144.t1 MTR_4g094525 60.169 236 62 2 1 235 1 205 1.07e-89 263
MS.gene02144.t1 MTR_4g094522 69.565 115 35 0 1 115 1 115 1.74e-47 153
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02144.t1 AT2G34770 75.847 236 57 0 1 236 1 236 5.89e-135 379
MS.gene02144.t1 AT4G20870 71.610 236 67 0 1 236 1 236 2.29e-126 357
MS.gene02144.t1 AT4G20870 71.610 236 67 0 1 236 1 236 2.29e-126 357

Find 73 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTTGGCGGCGGCTTAC+TGG 0.129077 2.2:-57289710 MS.gene02144:CDS
TCAACTACTCCTGCTTTATT+TGG 0.215512 2.2:-57289724 MS.gene02144:CDS
AGGATCCAAGATAAAGGATT+TGG 0.271287 2.2:-57289127 MS.gene02144:CDS
TTTCGGGGATGTTCAGTCTT+TGG 0.281707 2.2:+57289640 None:intergenic
GGTGGGCAATACCAGTTATT+TGG 0.282521 2.2:-57291285 MS.gene02144:CDS
GACCATGGTGCAAGTAATAA+TGG 0.337316 2.2:+57289665 None:intergenic
TGGGATCACTTCATCACTTT+GGG 0.338931 2.2:-57289107 MS.gene02144:CDS
CACTTTAGGATCCAAGATAA+AGG 0.360540 2.2:-57289133 MS.gene02144:CDS
GTTCTATAGGTTGGACATCT+TGG 0.372108 2.2:-57291995 MS.gene02144:intron
ACCACTTGAACCATCACTTT+AGG 0.374306 2.2:-57289147 MS.gene02144:CDS
TGATCCCAAATCCTTTATCT+TGG 0.378096 2.2:+57289122 None:intergenic
ATAGCTCTGATGGTGGTTCT+TGG 0.379060 2.2:-57291202 MS.gene02144:CDS
TGTATATAACTCTATACAAA+TGG 0.396707 2.2:-57291242 MS.gene02144:CDS
GGATCCAAGATAAAGGATTT+GGG 0.400632 2.2:-57289126 MS.gene02144:CDS
AACAGCTATATTACTGTTCC+CGG 0.429435 2.2:-57290667 MS.gene02144:intron
TTATTTGGCGGCGGCTTACT+GGG 0.429866 2.2:-57289709 MS.gene02144:CDS
ACAACTGGAAGCCAAATAAC+TGG 0.431941 2.2:+57291274 None:intergenic
TATACAAACCAACATACAAC+TGG 0.433560 2.2:+57291259 None:intergenic
AGTCCTTGACCCTCACTGTC+TGG 0.439297 2.2:-57291306 MS.gene02144:CDS
TTGGGATCACTTCATCACTT+TGG 0.444052 2.2:-57289108 MS.gene02144:CDS
AACCATCCATGGGGTGTTTG+TGG 0.448233 2.2:+57290715 None:intergenic
ACTACTCCTGCTTTATTTGG+CGG 0.451521 2.2:-57289721 MS.gene02144:CDS
TCCCCGTTTCTTTCAAAATG+AGG 0.458220 2.2:-57291924 MS.gene02144:CDS
TCAAATCAACTACAAAGTCC+TGG 0.474615 2.2:+57292201 None:intergenic
GGACCTTCCTTGCTAACAAT+GGG 0.475219 2.2:+57291944 None:intergenic
TTTGGCTTCCAGTTGTATGT+TGG 0.477885 2.2:-57291267 MS.gene02144:CDS
AACAAGTCTCAAACCATCCA+TGG 0.483587 2.2:+57290704 None:intergenic
CTGTCCTCATATAGCTCTGA+TGG 0.487299 2.2:-57291212 MS.gene02144:CDS
CAGCACCAAGATGGTTGCCC+AGG 0.498002 2.2:-57292219 None:intergenic
ACATTAAAACAAAGACCTAC+TGG 0.502397 2.2:-57291129 MS.gene02144:intron
CAGCCCATTGTTAGCAAGGA+AGG 0.502882 2.2:-57291947 MS.gene02144:CDS
CCTTGACCCTCACTGTCTGG+TGG 0.505883 2.2:-57291303 MS.gene02144:CDS
AAGCCTGTACCTGGAAAACA+AGG 0.507542 2.2:+57292171 None:intergenic
AATATAGCTGTTGCAGTAGG+AGG 0.519735 2.2:+57290678 None:intergenic
GGGACCTTCCTTGCTAACAA+TGG 0.525396 2.2:+57291943 None:intergenic
GTATATAACTCTATACAAAT+GGG 0.525508 2.2:-57291241 MS.gene02144:CDS
GCCATGTAGAAGATAATGTA+TGG 0.525715 2.2:+57290743 None:intergenic
AGCAGGAGTAGTTGAAGGGG+TGG 0.532131 2.2:+57289732 None:intergenic
ACCATGGTGCAAGTAATAAT+GGG 0.532977 2.2:+57289666 None:intergenic
TATCTTGGATCCTAAAGTGA+TGG 0.534098 2.2:+57289137 None:intergenic
ACTCCTGCTTTATTTGGCGG+CGG 0.542089 2.2:-57289718 MS.gene02144:CDS
CTTGACCCTCACTGTCTGGT+GGG 0.548253 2.2:-57291302 MS.gene02144:CDS
AATAAAGCAGGAGTAGTTGA+AGG 0.548583 2.2:+57289727 None:intergenic
CAAATCAACTACAAAGTCCT+GGG 0.550444 2.2:+57292202 None:intergenic
CTTTCAAAATGAGGTTCTAG+AGG 0.555614 2.2:-57291915 MS.gene02144:intron
TTGGTGAAGCTTATGAAGAG+TGG 0.555652 2.2:-57291976 MS.gene02144:CDS
AAGCCGCCGCCAAATAAAGC+AGG 0.556526 2.2:+57289715 None:intergenic
AACACTTCCTTCCTCAAAAG+CGG 0.566327 2.2:-57289071 MS.gene02144:CDS
TCATCAGCCCATTGTTAGCA+AGG 0.566774 2.2:-57291951 MS.gene02144:CDS
GTTCAGTCTTTGGCTGACCA+TGG 0.566911 2.2:+57289650 None:intergenic
TGGTGAAGCTTATGAAGAGT+GGG 0.567564 2.2:-57291975 MS.gene02144:CDS
ACAAGTCTCAAACCATCCAT+GGG 0.570404 2.2:+57290705 None:intergenic
TCCTAAAGTGATGGTTCAAG+TGG 0.570815 2.2:+57289146 None:intergenic
CACCACAAACACCCCATGGA+TGG 0.571556 2.2:-57290717 MS.gene02144:CDS
ACCACCATCAGAGCTATATG+AGG 0.581427 2.2:+57291208 None:intergenic
CTGCCACCACAAACACCCCA+TGG 0.603098 2.2:-57290721 MS.gene02144:CDS
AGTAATATAGCTGTTGCAGT+AGG 0.614561 2.2:+57290675 None:intergenic
ATAAAGCAGGAGTAGTTGAA+GGG 0.615938 2.2:+57289728 None:intergenic
ATATAGCTGTTGCAGTAGGA+GGG 0.615997 2.2:+57290679 None:intergenic
ACCATACATTATCTTCTACA+TGG 0.618370 2.2:-57290744 MS.gene02144:CDS
GCCTGTACCTGGAAAACAAG+GGG 0.619950 2.2:+57292173 None:intergenic
ACCCATTATTACTTGCACCA+TGG 0.623569 2.2:-57289667 MS.gene02144:CDS
TGAACATCCCCGAAACCTGA+AGG 0.626671 2.2:-57289632 MS.gene02144:intron
CATCCATGGGGTGTTTGTGG+TGG 0.632413 2.2:+57290718 None:intergenic
CGGACTCCAGTTCTGGAACG+TGG 0.633072 2.2:-57289767 MS.gene02144:intron
TCCTCATATAGCTCTGATGG+TGG 0.637299 2.2:-57291209 MS.gene02144:CDS
CCACCAGACAGTGAGGGTCA+AGG 0.641225 2.2:+57291303 None:intergenic
GAGCTATATGAGGACAGCTG+AGG 0.663833 2.2:+57291218 None:intergenic
TATTGCCCACCAGACAGTGA+GGG 0.672832 2.2:+57291297 None:intergenic
TAAAGCAGGAGTAGTTGAAG+GGG 0.675171 2.2:+57289729 None:intergenic
GTATTGCCCACCAGACAGTG+AGG 0.692936 2.2:+57291296 None:intergenic
AGCCTGTACCTGGAAAACAA+GGG 0.707081 2.2:+57292172 None:intergenic
CAAGTCTCAAACCATCCATG+GGG 0.739606 2.2:+57290706 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAACAAATTTAAAAAAGAA+TGG + chr2.2:57290870-57290889 None:intergenic 10.0%
!! TACTAACATTATTTATTATT+GGG + chr2.2:57290354-57290373 None:intergenic 10.0%
!! TTACTAACATTATTTATTAT+TGG + chr2.2:57290355-57290374 None:intergenic 10.0%
!!! TTTATAAAAAAACAAGTTTT+TGG - chr2.2:57289477-57289496 MS.gene02144:intron 10.0%
!! GATTCTTCAATAATAATTTA+AGG - chr2.2:57291194-57291213 MS.gene02144:CDS 15.0%
!! TAACGTTTAAAAACATAATT+TGG - chr2.2:57291037-57291056 MS.gene02144:intron 15.0%
!! TAATATGGTAGATTATTAAA+AGG + chr2.2:57289858-57289877 None:intergenic 15.0%
!!! AAAAAAACTAATTACTCTTT+TGG + chr2.2:57290738-57290757 None:intergenic 15.0%
!!! ACAAATTTTTAATGTTCTAT+AGG - chr2.2:57289255-57289274 MS.gene02144:intron 15.0%
!!! ATTTTTCTTATTATGACAAA+TGG - chr2.2:57290981-57291000 MS.gene02144:intron 15.0%
!! AAAAAAAAATCGAACCTAAT+TGG + chr2.2:57290911-57290930 None:intergenic 20.0%
!! AATGTAAATAAGATACGTAA+TGG - chr2.2:57291932-57291951 MS.gene02144:CDS 20.0%
!! AATTTGTATTTAATCTCATG+TGG - chr2.2:57291162-57291181 MS.gene02144:CDS 20.0%
!! ACTTAAATAGTAAGATTAGA+TGG - chr2.2:57291006-57291025 MS.gene02144:intron 20.0%
!! ATATTGACATATCTATATTG+AGG + chr2.2:57291404-57291423 None:intergenic 20.0%
!! ATGTAAATAAGATACGTAAT+GGG - chr2.2:57291933-57291952 MS.gene02144:CDS 20.0%
!! ATTTGTATTTAATCTCATGT+GGG - chr2.2:57291163-57291182 MS.gene02144:CDS 20.0%
!! CAATATAGATATGTCAATAT+TGG - chr2.2:57291404-57291423 MS.gene02144:intron 20.0%
!! GTATATAACTCTATACAAAT+GGG - chr2.2:57290022-57290041 MS.gene02144:intron 20.0%
!! GTTTAAAAACATAATTTGGA+AGG - chr2.2:57291041-57291060 MS.gene02144:intron 20.0%
!! TCCAATAAAAATCAATGAAA+AGG - chr2.2:57291743-57291762 MS.gene02144:intron 20.0%
!! TGTATATAACTCTATACAAA+TGG - chr2.2:57290021-57290040 MS.gene02144:intron 20.0%
!! TTTATTTATTTGGTACCTTT+CGG - chr2.2:57292011-57292030 MS.gene02144:intron 20.0%
!!! AACAAATAAATTGTTGTTTC+TGG - chr2.2:57291253-57291272 MS.gene02144:CDS 20.0%
!!! AGATAGCGAAATAATTTTTT+TGG + chr2.2:57289569-57289588 None:intergenic 20.0%
!!! ATGAAATCATAAGTTGATTT+AGG - chr2.2:57290194-57290213 MS.gene02144:intron 20.0%
!!! ATTTGATGAAAATTTGTTGT+AGG + chr2.2:57290935-57290954 None:intergenic 20.0%
!!! ATTTTTAATGTTCTATAGGT+TGG - chr2.2:57289259-57289278 MS.gene02144:intron 20.0%
!!! CTGTTTTAAAAACAAATATC+CGG + chr2.2:57291102-57291121 None:intergenic 20.0%
!!! CTTCTATCACTTTATTTATT+TGG - chr2.2:57292001-57292020 MS.gene02144:intron 20.0%
!!! TCCTTTTCATTGATTTTTAT+TGG + chr2.2:57291747-57291766 None:intergenic 20.0%
!!! TTAATTTTATGTGTAGAGTT+AGG - chr2.2:57289176-57289195 MS.gene02144:intron 20.0%
!!! TTTTAATGTGGAAAAGAAAA+CGG + chr2.2:57290123-57290142 None:intergenic 20.0%
!!! TTTTTATCATGAATTCTTCT+TGG - chr2.2:57290695-57290714 MS.gene02144:CDS 20.0%
! AAAATTCCATGAAGTAATGT+TGG - chr2.2:57291459-57291478 MS.gene02144:intron 25.0%
! ATATGGCAAAAAATAGATAC+CGG + chr2.2:57290618-57290637 None:intergenic 25.0%
! ATCTTCATTTGCTTGTAATA+TGG + chr2.2:57289873-57289892 None:intergenic 25.0%
! TATCGTAATAAAATACATGC+TGG - chr2.2:57290251-57290270 MS.gene02144:intron 25.0%
! TGAAAATTCGGCATAAAATA+TGG + chr2.2:57290635-57290654 None:intergenic 25.0%
! TGAATTTCAGTAGATATTGA+AGG - chr2.2:57291833-57291852 MS.gene02144:intron 25.0%
! TTCATCAAATTACTCCTTTA+AGG - chr2.2:57290945-57290964 MS.gene02144:intron 25.0%
! TTGAAATCTAATACATGACA+TGG + chr2.2:57289142-57289161 None:intergenic 25.0%
! TTGAAGACAAAGTTAAAAGA+AGG + chr2.2:57291914-57291933 None:intergenic 25.0%
!! GATTTTGCTTAGCAAAATAT+TGG + chr2.2:57291716-57291735 None:intergenic 25.0%
!!! AGCGAAATAATTTTTTTGGA+GGG + chr2.2:57289565-57289584 None:intergenic 25.0%
!!! ATGTTTCCTTTGATTTTGAT+TGG - chr2.2:57289794-57289813 MS.gene02144:intron 25.0%
!!! CAAGAAGGTATTTGAAAATT+CGG + chr2.2:57290647-57290666 None:intergenic 25.0%
!!! CATTTAACCAAAAAGCATTA+CGG + chr2.2:57290483-57290502 None:intergenic 25.0%
!!! TAAGTTGATTTAGGAAATGT+TGG - chr2.2:57290203-57290222 MS.gene02144:intron 25.0%
!!! TAGCGAAATAATTTTTTTGG+AGG + chr2.2:57289566-57289585 None:intergenic 25.0%
!!! TTTGGTTAAATGTTGCTTTA+GGG - chr2.2:57290491-57290510 MS.gene02144:intron 25.0%
!!! TTTTGGTTAAATGTTGCTTT+AGG - chr2.2:57290490-57290509 MS.gene02144:intron 25.0%
AAAACACCAACATTACTTCA+TGG + chr2.2:57291468-57291487 None:intergenic 30.0%
AAAATAACAATTGTGCCAAC+TGG + chr2.2:57291886-57291905 None:intergenic 30.0%
AACATGCCAATCAAAATCAA+AGG + chr2.2:57289803-57289822 None:intergenic 30.0%
ACATTAAAACAAAGACCTAC+TGG - chr2.2:57290134-57290153 MS.gene02144:intron 30.0%
ACCATACATTATCTTCTACA+TGG - chr2.2:57290519-57290538 MS.gene02144:intron 30.0%
ACCGTAAAACTAGAAATACA+CGG + chr2.2:57289738-57289757 None:intergenic 30.0%
AGGATTAGAAGTGATTATTC+AGG + chr2.2:57289706-57289725 None:intergenic 30.0%
AGTTATTAGCTGATCAAAGT+CGG - chr2.2:57291790-57291809 MS.gene02144:intron 30.0%
AGTTTCATCATGTGAGAAAA+AGG + chr2.2:57289628-57289647 None:intergenic 30.0%
ATATTTCTTCATAGCCAAGA+AGG + chr2.2:57290662-57290681 None:intergenic 30.0%
CAAAATATTGGAGTAACGAA+TGG + chr2.2:57291704-57291723 None:intergenic 30.0%
CAACTTATGATTTCATGCTA+TGG + chr2.2:57290190-57290209 None:intergenic 30.0%
GAAATACACGGAAACAATAT+AGG + chr2.2:57289726-57289745 None:intergenic 30.0%
GGATTAGAAGTGATTATTCA+GGG + chr2.2:57289705-57289724 None:intergenic 30.0%
GTTTACAAGACGGAAATAAT+TGG + chr2.2:57292055-57292074 None:intergenic 30.0%
TATACAAACCAACATACAAC+TGG + chr2.2:57290007-57290026 None:intergenic 30.0%
TATGGCAAAAAATAGATACC+GGG + chr2.2:57290617-57290636 None:intergenic 30.0%
TGTCCCACTATATAAAAAGT+GGG - chr2.2:57290293-57290312 MS.gene02144:intron 30.0%
TTCAATGTGAAACTCCTTAA+AGG + chr2.2:57290962-57290981 None:intergenic 30.0%
TTCATGCTATGGAAACAAAA+GGG + chr2.2:57290179-57290198 None:intergenic 30.0%
TTTCATGCTATGGAAACAAA+AGG + chr2.2:57290180-57290199 None:intergenic 30.0%
! AAGTTTACCGTAATGCTTTT+TGG - chr2.2:57290473-57290492 MS.gene02144:intron 30.0%
! ACGGTAATGATAAAGTTTTC+TGG - chr2.2:57289753-57289772 MS.gene02144:CDS 30.0%
! ACTTGAATTTTCCCTTTTGA+GGG + chr2.2:57290763-57290782 None:intergenic 30.0%
! ATTTTATACCCTTCTTCGAT+AGG - chr2.2:57291219-57291238 MS.gene02144:CDS 30.0%
! CATGAATTCTTCTTGGTAAT+AGG - chr2.2:57290702-57290721 MS.gene02144:CDS 30.0%
! CGAATTTTCAAATACCTTCT+TGG - chr2.2:57290645-57290664 MS.gene02144:intron 30.0%
! TCTTCTTGGTAATAGGAATT+TGG - chr2.2:57290709-57290728 MS.gene02144:CDS 30.0%
!! AACCTCATTTTGAAAGAAAC+GGG + chr2.2:57289344-57289363 None:intergenic 30.0%
!! CATGGAGAAATTTTTGAAAG+AGG + chr2.2:57289124-57289143 None:intergenic 30.0%
!! GAAATAATTGGTTGGTAACT+TGG + chr2.2:57292043-57292062 None:intergenic 30.0%
!! GAACCTCATTTTGAAAGAAA+CGG + chr2.2:57289345-57289364 None:intergenic 30.0%
!! TTGGTTAAATGTTGCTTTAG+GGG - chr2.2:57290492-57290511 MS.gene02144:intron 30.0%
!! TTTAAAACCTCCGTTTTGTA+AGG - chr2.2:57290818-57290837 MS.gene02144:intron 30.0%
!!! AAGTCCCACTTTTTATATAG+TGG + chr2.2:57290300-57290319 None:intergenic 30.0%
!!! AGTAGGTCTTTGTTTTAATG+TGG + chr2.2:57290135-57290154 None:intergenic 30.0%
!!! AGTCCCACTTTTTATATAGT+GGG + chr2.2:57290299-57290318 None:intergenic 30.0%
!!! AGTTTTTTTTCCCCTCAAAA+GGG - chr2.2:57290749-57290768 MS.gene02144:CDS 30.0%
!!! TAATTTTTTTGGAGGGACTA+CGG + chr2.2:57289558-57289577 None:intergenic 30.0%
!!! TAGTTTTTTTTCCCCTCAAA+AGG - chr2.2:57290748-57290767 MS.gene02144:CDS 30.0%
!!! TCACTTTGGGATAAAGTTTT+TGG - chr2.2:57292169-57292188 MS.gene02144:intron 30.0%
!!! TCCGTGTATTTCTAGTTTTA+CGG - chr2.2:57289734-57289753 MS.gene02144:CDS 30.0%
AAAACCAGGGAAAGATGATT+AGG + chr2.2:57292086-57292105 None:intergenic 35.0%
AAAATCGAACCTAATTGGTG+AGG + chr2.2:57290906-57290925 None:intergenic 35.0%
AAATTAACAACCCTTAGCAG+AGG + chr2.2:57291975-57291994 None:intergenic 35.0%
AAATTGCCATCCACAGTTAA+AGG + chr2.2:57291676-57291695 None:intergenic 35.0%
AACAGCTATATTACTGTTCC+CGG - chr2.2:57290596-57290615 MS.gene02144:intron 35.0%
AATAAAGCAGGAGTAGTTGA+AGG + chr2.2:57291539-57291558 None:intergenic 35.0%
ACAAGACGGAAATAATTGGT+TGG + chr2.2:57292051-57292070 None:intergenic 35.0%
ACCATGGTGCAAGTAATAAT+GGG + chr2.2:57291600-57291619 None:intergenic 35.0%
AGACAAAGTTAAAAGAAGGC+AGG + chr2.2:57291910-57291929 None:intergenic 35.0%
AGGATCCAAGATAAAGGATT+TGG - chr2.2:57292136-57292155 MS.gene02144:intron 35.0%
AGTAATATAGCTGTTGCAGT+AGG + chr2.2:57290591-57290610 None:intergenic 35.0%
ATAAAGCAGGAGTAGTTGAA+GGG + chr2.2:57291538-57291557 None:intergenic 35.0%
ATTTGGAAGGAAAATCACCA+TGG - chr2.2:57291054-57291073 MS.gene02144:intron 35.0%
CAAATCAACTACAAAGTCCT+GGG + chr2.2:57289064-57289083 None:intergenic 35.0%
CAGAGTGAAGTATAAGAATG+TGG - chr2.2:57291119-57291138 MS.gene02144:intron 35.0%
CTGGTTACATACACATATCT+AGG + chr2.2:57291867-57291886 None:intergenic 35.0%
GACTTTGATCAGCTAATAAC+TGG + chr2.2:57291791-57291810 None:intergenic 35.0%
GATATGTGTATGTAACCAGT+TGG - chr2.2:57291868-57291887 MS.gene02144:intron 35.0%
GCCATGTAGAAGATAATGTA+TGG + chr2.2:57290523-57290542 None:intergenic 35.0%
GGAAAATTCAAGTCTCACAT+TGG - chr2.2:57290770-57290789 MS.gene02144:intron 35.0%
GGATCCAAGATAAAGGATTT+GGG - chr2.2:57292137-57292156 MS.gene02144:intron 35.0%
GGCAGATCAGTTAATGATAT+TGG - chr2.2:57289453-57289472 MS.gene02144:intron 35.0%
GGCTTGTATAGTTTACAAGA+CGG + chr2.2:57292065-57292084 None:intergenic 35.0%
GTGTCCCACTATATAAAAAG+TGG - chr2.2:57290292-57290311 MS.gene02144:intron 35.0%
GTGTTATAGTAGTACACAAC+TGG + chr2.2:57291315-57291334 None:intergenic 35.0%
TATCTTGGATCCTAAAGTGA+TGG + chr2.2:57292129-57292148 None:intergenic 35.0%
TATTTGAAAGCCTCTGCTAA+GGG - chr2.2:57291962-57291981 MS.gene02144:CDS 35.0%
TCAAATCAACTACAAAGTCC+TGG + chr2.2:57289065-57289084 None:intergenic 35.0%
TCACATGATGAAACTTCACT+GGG - chr2.2:57289633-57289652 MS.gene02144:CDS 35.0%
TCTAACTCAGCCTTACAAAA+CGG + chr2.2:57290831-57290850 None:intergenic 35.0%
TGATCCCAAATCCTTTATCT+TGG + chr2.2:57292144-57292163 None:intergenic 35.0%
TTATTTGAAAGCCTCTGCTA+AGG - chr2.2:57291961-57291980 MS.gene02144:CDS 35.0%
TTTGGAAGGAAAATCACCAT+GGG - chr2.2:57291055-57291074 MS.gene02144:intron 35.0%
! CTTGAATTTTCCCTTTTGAG+GGG + chr2.2:57290762-57290781 None:intergenic 35.0%
! CTTTCAAAATGAGGTTCTAG+AGG - chr2.2:57289348-57289367 MS.gene02144:intron 35.0%
! GACTTGAATTTTCCCTTTTG+AGG + chr2.2:57290764-57290783 None:intergenic 35.0%
! TCAACTACTCCTGCTTTATT+TGG - chr2.2:57291539-57291558 MS.gene02144:intron 35.0%
!! ACCTCATTTTGAAAGAAACG+GGG + chr2.2:57289343-57289362 None:intergenic 35.0%
!! CACTTTAGGATCCAAGATAA+AGG - chr2.2:57292130-57292149 MS.gene02144:intron 35.0%
!! GTACTTGACTTGTTACTGTT+TGG - chr2.2:57289931-57289950 MS.gene02144:intron 35.0%
!! TGTTGGTGTTTTGTATTTCG+CGG - chr2.2:57291476-57291495 MS.gene02144:intron 35.0%
!!! CACGTGTCTACTTTTTATCA+GGG - chr2.2:57289591-57289610 MS.gene02144:intron 35.0%
AACAAGTCTCAAACCATCCA+TGG + chr2.2:57290562-57290581 None:intergenic 40.0%
AACACTTCCTTCCTCAAAAG+CGG - chr2.2:57292192-57292211 MS.gene02144:CDS 40.0%
AATATAGCTGTTGCAGTAGG+AGG + chr2.2:57290588-57290607 None:intergenic 40.0%
ACAACTGGAAGCCAAATAAC+TGG + chr2.2:57289992-57290011 None:intergenic 40.0%
ACAAGTCTCAAACCATCCAT+GGG + chr2.2:57290561-57290580 None:intergenic 40.0%
ACCACTTGAACCATCACTTT+AGG - chr2.2:57292116-57292135 MS.gene02144:intron 40.0%
ACCCATTATTACTTGCACCA+TGG - chr2.2:57291596-57291615 MS.gene02144:intron 40.0%
ATATAGCTGTTGCAGTAGGA+GGG + chr2.2:57290587-57290606 None:intergenic 40.0%
ATCCACAGTTAAAGGAGGAA+GGG + chr2.2:57291668-57291687 None:intergenic 40.0%
CCTGATAAAAAGTAGACACG+TGG + chr2.2:57289593-57289612 None:intergenic 40.0%
CTCACATGATGAAACTTCAC+TGG - chr2.2:57289632-57289651 MS.gene02144:intron 40.0%
CTTAAGCTCCTATCGAAGAA+GGG + chr2.2:57291230-57291249 None:intergenic 40.0%
GACCATGGTGCAAGTAATAA+TGG + chr2.2:57291601-57291620 None:intergenic 40.0%
GTAACTTGGTATTCACCGAA+AGG + chr2.2:57292029-57292048 None:intergenic 40.0%
GTGGGACTTACATGATTTAG+TGG - chr2.2:57290311-57290330 MS.gene02144:intron 40.0%
TAAAGCAGGAGTAGTTGAAG+GGG + chr2.2:57291537-57291556 None:intergenic 40.0%
TCCCCGTTTCTTTCAAAATG+AGG - chr2.2:57289339-57289358 MS.gene02144:intron 40.0%
TCCTAAAGTGATGGTTCAAG+TGG + chr2.2:57292120-57292139 None:intergenic 40.0%
TCTTAAGCTCCTATCGAAGA+AGG + chr2.2:57291231-57291250 None:intergenic 40.0%
TGGGATCACTTCATCACTTT+GGG - chr2.2:57292156-57292175 MS.gene02144:intron 40.0%
TGGTGAAGCTTATGAAGAGT+GGG - chr2.2:57289288-57289307 MS.gene02144:intron 40.0%
TTGGGATCACTTCATCACTT+TGG - chr2.2:57292155-57292174 MS.gene02144:intron 40.0%
TTGGTGAAGCTTATGAAGAG+TGG - chr2.2:57289287-57289306 MS.gene02144:intron 40.0%
! CAGCTAAGTCTTGTATCACT+TGG - chr2.2:57289394-57289413 MS.gene02144:intron 40.0%
! GTTAGGTTGTGACAACAAGT+AGG - chr2.2:57289193-57289212 MS.gene02144:intron 40.0%
! GTTCTATAGGTTGGACATCT+TGG - chr2.2:57289268-57289287 MS.gene02144:intron 40.0%
! TGCTCTGAGTAACAGCTTAT+TGG - chr2.2:57290434-57290453 MS.gene02144:intron 40.0%
! TGGTTCTTGGCATTTTTGTC+TGG - chr2.2:57290074-57290093 MS.gene02144:intron 40.0%
! TTTGGCTTCCAGTTGTATGT+TGG - chr2.2:57289996-57290015 MS.gene02144:intron 40.0%
!! ACTACTCCTGCTTTATTTGG+CGG - chr2.2:57291542-57291561 MS.gene02144:intron 40.0%
!! AGTCTTGTATCACTTGGTGT+AGG - chr2.2:57289400-57289419 MS.gene02144:intron 40.0%
!! ATGCTGGTTTCCCTAAAAAC+AGG - chr2.2:57290267-57290286 MS.gene02144:intron 40.0%
!! GTCTTGTATCACTTGGTGTA+GGG - chr2.2:57289401-57289420 MS.gene02144:intron 40.0%
!! GTGCTTTTACCTTCAGGTTT+CGG + chr2.2:57291643-57291662 None:intergenic 40.0%
!! TCTTGTATCACTTGGTGTAG+GGG - chr2.2:57289402-57289421 MS.gene02144:intron 40.0%
!! TGCTGGTTTCCCTAAAAACA+GGG - chr2.2:57290268-57290287 MS.gene02144:intron 40.0%
!! TGCTTTTACCTTCAGGTTTC+GGG + chr2.2:57291642-57291661 None:intergenic 40.0%
!!! CCACGTGTCTACTTTTTATC+AGG - chr2.2:57289590-57289609 MS.gene02144:intron 40.0%
AAACTTCACTGGGCGAGTTT+CGG - chr2.2:57289643-57289662 MS.gene02144:CDS 45.0%
AAAGAATGGCTGACATCCAC+AGG + chr2.2:57290856-57290875 None:intergenic 45.0%
AACTCAGCCTTACAAAACGG+AGG + chr2.2:57290828-57290847 None:intergenic 45.0%
AAGCCTGTACCTGGAAAACA+AGG + chr2.2:57289095-57289114 None:intergenic 45.0%
AAGGCTGAGTTAGAAACCTG+TGG - chr2.2:57290837-57290856 MS.gene02144:intron 45.0%
AATCACCATGGGCACAACAT+TGG - chr2.2:57291066-57291085 MS.gene02144:intron 45.0%
ACCACCATCAGAGCTATATG+AGG + chr2.2:57290058-57290077 None:intergenic 45.0%
AGCCTGTACCTGGAAAACAA+GGG + chr2.2:57289094-57289113 None:intergenic 45.0%
AGTAGTACACAACTGGAGTC+AGG + chr2.2:57291308-57291327 None:intergenic 45.0%
CAAGCCTAATCATCTTTCCC+TGG - chr2.2:57292079-57292098 MS.gene02144:intron 45.0%
CAAGTCTCAAACCATCCATG+GGG + chr2.2:57290560-57290579 None:intergenic 45.0%
CAGAGAGAGATCTAACCAGT+AGG + chr2.2:57290152-57290171 None:intergenic 45.0%
CATCCACAGTTAAAGGAGGA+AGG + chr2.2:57291669-57291688 None:intergenic 45.0%
CTTCCTCCTTTAACTGTGGA+TGG - chr2.2:57291667-57291686 MS.gene02144:intron 45.0%
GATACAAGACTTAGCTGTCG+TGG + chr2.2:57289391-57289410 None:intergenic 45.0%
GCTGTTACTCAGAGCAGATA+TGG + chr2.2:57290430-57290449 None:intergenic 45.0%
GGACCTTCCTTGCTAACAAT+GGG + chr2.2:57289322-57289341 None:intergenic 45.0%
GGTACTTCTGCAGAAAACCA+GGG + chr2.2:57292099-57292118 None:intergenic 45.0%
GGTGGGCAATACCAGTTATT+TGG - chr2.2:57289978-57289997 MS.gene02144:intron 45.0%
GGTTTCTCACCTCACCAATT+AGG - chr2.2:57290894-57290913 MS.gene02144:intron 45.0%
TCATCAGCCCATTGTTAGCA+AGG - chr2.2:57289312-57289331 MS.gene02144:intron 45.0%
TGGTACTTCTGCAGAAAACC+AGG + chr2.2:57292100-57292119 None:intergenic 45.0%
TTCCCTTCCTCCTTTAACTG+TGG - chr2.2:57291663-57291682 MS.gene02144:intron 45.0%
TTGCCATCCACAGTTAAAGG+AGG + chr2.2:57291673-57291692 None:intergenic 45.0%
TTTCGGGGATGTTCAGTCTT+TGG + chr2.2:57291626-57291645 None:intergenic 45.0%
! AGGACTTTTAAGCCTGTACC+TGG + chr2.2:57289104-57289123 None:intergenic 45.0%
! CAACATTGGCTGTTTGAAGC+CGG - chr2.2:57291080-57291099 MS.gene02144:intron 45.0%
!! ATAGCTCTGATGGTGGTTCT+TGG - chr2.2:57290061-57290080 MS.gene02144:intron 45.0%
!! CTGTCCTCATATAGCTCTGA+TGG - chr2.2:57290051-57290070 MS.gene02144:intron 45.0%
!! GCTTTTACCTTCAGGTTTCG+GGG + chr2.2:57291641-57291660 None:intergenic 45.0%
!! GCTTTTAGCATCCGCTTTTG+AGG + chr2.2:57292206-57292225 None:intergenic 45.0%
!! TCCTCATATAGCTCTGATGG+TGG - chr2.2:57290054-57290073 MS.gene02144:intron 45.0%
!! TTAGCATCCGCTTTTGAGGA+AGG + chr2.2:57292202-57292221 None:intergenic 45.0%
!!! AGGGAAGTGCTTTTACCTTC+AGG + chr2.2:57291649-57291668 None:intergenic 45.0%
!!! GAATAAGCCCCTTGTTTTCC+AGG - chr2.2:57289083-57289102 MS.gene02144:CDS 45.0%
!!! TGGGACACACCCTGTTTTTA+GGG + chr2.2:57290280-57290299 None:intergenic 45.0%
!!! TTTTTTAAATTTGTTTAATT+TGG - chr2.2:57290873-57290892 MS.gene02144:intron 5.0%
AACAGCCAATGTTGTGCCCA+TGG + chr2.2:57291074-57291093 None:intergenic 50.0%
CAGCCCATTGTTAGCAAGGA+AGG - chr2.2:57289316-57289335 MS.gene02144:intron 50.0%
GAGCTATATGAGGACAGCTG+AGG + chr2.2:57290048-57290067 None:intergenic 50.0%
GCCTGTACCTGGAAAACAAG+GGG + chr2.2:57289093-57289112 None:intergenic 50.0%
GGGACCTTCCTTGCTAACAA+TGG + chr2.2:57289323-57289342 None:intergenic 50.0%
GTTCAGTCTTTGGCTGACCA+TGG + chr2.2:57291616-57291635 None:intergenic 50.0%
TATTGCCCACCAGACAGTGA+GGG + chr2.2:57289969-57289988 None:intergenic 50.0%
TATTTCGCGGACTCCAGTTC+TGG - chr2.2:57291489-57291508 MS.gene02144:intron 50.0%
TGAACATCCCCGAAACCTGA+AGG - chr2.2:57291631-57291650 MS.gene02144:intron 50.0%
TTATTTGGCGGCGGCTTACT+GGG - chr2.2:57291554-57291573 MS.gene02144:intron 50.0%
TTTATTTGGCGGCGGCTTAC+TGG - chr2.2:57291553-57291572 MS.gene02144:intron 50.0%
! AACCATCCATGGGGTGTTTG+TGG + chr2.2:57290551-57290570 None:intergenic 50.0%
!! ACTCCTGCTTTATTTGGCGG+CGG - chr2.2:57291545-57291564 MS.gene02144:intron 50.0%
!! GTATCACTTGGTGTAGGGGT+GGG - chr2.2:57289406-57289425 MS.gene02144:intron 50.0%
!! GTGGGACACACCCTGTTTTT+AGG + chr2.2:57290281-57290300 None:intergenic 50.0%
!! TATCACTTGGTGTAGGGGTG+GGG - chr2.2:57289407-57289426 MS.gene02144:intron 50.0%
!! TGTATCACTTGGTGTAGGGG+TGG - chr2.2:57289405-57289424 MS.gene02144:intron 50.0%
AAGCCGCCGCCAAATAAAGC+AGG + chr2.2:57291551-57291570 None:intergenic 55.0%
AGCAGGAGTAGTTGAAGGGG+TGG + chr2.2:57291534-57291553 None:intergenic 55.0%
AGTCCTTGACCCTCACTGTC+TGG - chr2.2:57289957-57289976 MS.gene02144:intron 55.0%
CACCACAAACACCCCATGGA+TGG - chr2.2:57290546-57290565 MS.gene02144:intron 55.0%
CTTGACCCTCACTGTCTGGT+GGG - chr2.2:57289961-57289980 MS.gene02144:intron 55.0%
GCTTCACCACGTTCCAGAAC+TGG + chr2.2:57291505-57291524 None:intergenic 55.0%
GGAGATTGCTAGGCTAGGCT+AGG - chr2.2:57289432-57289451 MS.gene02144:intron 55.0%
GTATTGCCCACCAGACAGTG+AGG + chr2.2:57289970-57289989 None:intergenic 55.0%
! CATCCATGGGGTGTTTGTGG+TGG + chr2.2:57290548-57290567 None:intergenic 55.0%
!! ATCACTTGGTGTAGGGGTGG+GGG - chr2.2:57289408-57289427 MS.gene02144:intron 55.0%
!!! GCCCCTTGTTTTCCAGGTAC+AGG - chr2.2:57289089-57289108 MS.gene02144:CDS 55.0%
CCACCAGACAGTGAGGGTCA+AGG + chr2.2:57289963-57289982 None:intergenic 60.0%
CCTTGACCCTCACTGTCTGG+TGG - chr2.2:57289960-57289979 MS.gene02144:intron 60.0%
CTGCCACCACAAACACCCCA+TGG - chr2.2:57290542-57290561 MS.gene02144:intron 60.0%
GGGGTGGAGATTGCTAGGCT+AGG - chr2.2:57289427-57289446 MS.gene02144:intron 60.0%
!! CGGACTCCAGTTCTGGAACG+TGG - chr2.2:57291496-57291515 MS.gene02144:intron 60.0%
GGGTGGGGGTGGAGATTGCT+AGG - chr2.2:57289422-57289441 MS.gene02144:intron 65.0%
!! ACTTGGTGTAGGGGTGGGGG+TGG - chr2.2:57289411-57289430 MS.gene02144:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 57289054 57292231 57289054 ID=MS.gene02144
chr2.2 mRNA 57289054 57292231 57289054 ID=MS.gene02144.t1;Parent=MS.gene02144
chr2.2 exon 57292181 57292231 57292181 ID=MS.gene02144.t1.exon1;Parent=MS.gene02144.t1
chr2.2 CDS 57292181 57292231 57292181 ID=cds.MS.gene02144.t1;Parent=MS.gene02144.t1
chr2.2 exon 57291916 57292008 57291916 ID=MS.gene02144.t1.exon2;Parent=MS.gene02144.t1
chr2.2 CDS 57291916 57292008 57291916 ID=cds.MS.gene02144.t1;Parent=MS.gene02144.t1
chr2.2 exon 57291130 57291326 57291130 ID=MS.gene02144.t1.exon3;Parent=MS.gene02144.t1
chr2.2 CDS 57291130 57291326 57291130 ID=cds.MS.gene02144.t1;Parent=MS.gene02144.t1
chr2.2 exon 57290668 57290773 57290668 ID=MS.gene02144.t1.exon4;Parent=MS.gene02144.t1
chr2.2 CDS 57290668 57290773 57290668 ID=cds.MS.gene02144.t1;Parent=MS.gene02144.t1
chr2.2 exon 57289633 57289779 57289633 ID=MS.gene02144.t1.exon5;Parent=MS.gene02144.t1
chr2.2 CDS 57289633 57289779 57289633 ID=cds.MS.gene02144.t1;Parent=MS.gene02144.t1
chr2.2 exon 57289054 57289173 57289054 ID=MS.gene02144.t1.exon6;Parent=MS.gene02144.t1
chr2.2 CDS 57289054 57289173 57289054 ID=cds.MS.gene02144.t1;Parent=MS.gene02144.t1
Gene Sequence

>MS.gene02144

ATGGTTGCCCAGGACTTTGTAGTTGATTTGAATAAGCCCCTTGTTTTCCAGGTACAGGCTTAAAAGTCCTCTTTCAAAAATTTCTCCATGTCATGTATTAGATTTCAAATGTTACGCATTCGTTAATTTTATGTGTAGAGTTAGGTTGTGACAACAAGTAGGTAGTTTGTACTTTGTATTAGTAATTTCAAAAGAACTTGAACAAATTTTTAATGTTCTATAGGTTGGACATCTTGGTGAAGCTTATGAAGAGTGGGTTCATCAGCCCATTGTTAGCAAGGAAGGTCCCCGTTTCTTTCAAAATGAGGTTCTAGAGGTATATCATTGAACATGTCCACGACAGCTAAGTCTTGTATCACTTGGTGTAGGGGTGGGGGTGGAGATTGCTAGGCTAGGCTAGGCAGATCAGTTAATGATATTGGATTTATAAAAAAACAAGTTTTTGGTAAAAATGTTTAGAGCAATGTCTTTTTCAAAGATTTCTTCACAATTTTTCTATGACCGTAGTCCCTCCAAAAAAATTATTTCGCTATCTTCCACGTGTCTACTTTTTATCAGGGATTTTTATTTGCCTTTTTCTCACATGATGAAACTTCACTGGGCGAGTTTCGGTTATCTTGTCATGAATAGTCTCTAATCTAAGTTTCTCCCTGAATAATCACTTCTAATCCTATATTGTTTCCGTGTATTTCTAGTTTTACGGTAATGATAAAGTTTTCTGGTTATATAGCTTTTGATAAATGTTTCCTTTGATTTTGATTGGCATGTTGCAACTAAAGCGAACACGTTGCTATCTTCAATCCTTTTAATAATCTACCATATTACAAGCAAATGAAGATTTTAATTTGTATGTTCTGTTATACATTTAATATCATGCGTACTTGACTTGTTACTGTTTGGAACAGTCCTTGACCCTCACTGTCTGGTGGGCAATACCAGTTATTTGGCTTCCAGTTGTATGTTGGTTTGTATATAACTCTATACAAATGGGCCTCAGCTGTCCTCATATAGCTCTGATGGTGGTTCTTGGCATTTTTGTCTGGACATTGCTTGAGTATACATTGCACCGTTTTCTTTTCCACATTAAAACAAAGACCTACTGGTTAGATCTCTCTCTGTGTTCCCTTTTGTTTCCATAGCATGAAATCATAAGTTGATTTAGGAAATGTTGGCAACACATTCTTTAACACACTCTATTATCGTAATAAAATACATGCTGGTTTCCCTAAAAACAGGGTGTGTCCCACTATATAAAAAGTGGGACTTACATGATTTAGTGGACTTGATAGAGATTTCACCCAATAATAAATAATGTTAGTAAAAGAATGTGTTGCTAGCGTTACTTATTAGTTTTTGCATGTAGTTGTTTTGAACCATATCTGCTCTGAGTAACAGCTTATTGGTATTGAATAACACTGAAAGTTTACCGTAATGCTTTTTGGTTAAATGTTGCTTTAGGGGAAATACCATACATTATCTTCTACATGGCTGCCACCACAAACACCCCATGGATGGTTTGAGACTTGTTTTCCCTCCTACTGCAACAGCTATATTACTGTTCCCGGTATCTATTTTTTGCCATATTTTATGCCGAATTTTCAAATACCTTCTTGGCTATGAAGAAATATGAGATTTCTTATGTTTTTATCATGAATTCTTCTTGGTAATAGGAATTTGGAGACCAAAAGAGTAATTAGTTTTTTTTCCCCTCAAAAGGGAAAATTCAAGTCTCACATTGGCTTGAGAAAAAGTCTAAGTACAGCTTTTAAAACCTCCGTTTTGTAAGGCTGAGTTAGAAACCTGTGGATGTCAGCCATTCTTTTTTAAATTTGTTTAATTTGGTTTCTCACCTCACCAATTAGGTTCGATTTTTTTTTTCCTACAACAAATTTTCATCAAATTACTCCTTTAAGGAGTTTCACATTGAATTTTTCTTATTATGACAAATGGTTACTTAAATAGTAAGATTAGATGGATTCTTTGTAACGTTTAAAAACATAATTTGGAAGGAAAATCACCATGGGCACAACATTGGCTGTTTGAAGCCGGATATTTGTTTTTAAAACAGAGTGAAGTATAAGAATGTGGATAAGATCTCACTAAAGTTGAATTTGTATTTAATCTCATGTGGGAAGACAATGATTCTTCAATAATAATTTAAGGATATTTTATACCCTTCTTCGATAGGAGCTTAAGAAAAACAAATAAATTGTTGTTTCTGGATTTATTGAGTGAAGTCACGACAGACTCTCCTGACTCCAGTTGTGTACTACTATAACACATTTTGTAGCTTTCTTTTCAAAATGCGAGAAGACTTTTTTACATTTTATAAAATTATTCGACTAATCCTCAATATAGATATGTCAATATTGGTAAGATTCAGATAATTTTTGCACACATAGTTTAAAATTCCATGAAGTAATGTTGGTGTTTTGTATTTCGCGGACTCCAGTTCTGGAACGTGGTGAAGCTCTTTTCCACCCCTTCAACTACTCCTGCTTTATTTGGCGGCGGCTTACTGGGTTATGTAATTTATGATTGCACCCATTATTACTTGCACCATGGTCAGCCAAAGACTGAACATCCCCGAAACCTGAAGGTAAAAGCACTTCCCTTCCTCCTTTAACTGTGGATGGCAATTTAATTACCATTCGTTACTCCAATATTTTGCTAAGCAAAATCATTTTGTTCCAATAAAAATCAATGAAAAGGATAAAAGCAGTTGTATAATTTACCAGTTATTAGCTGATCAAAGTCGGTTCTTCTAGCATTTAAGTATTGAATTTCAGTAGATATTGAAGGACATTAAACCTAGATATGTGTATGTAACCAGTTGGCACAATTGTTATTTTTCCTGCCTTCTTTTAACTTTGTCTTCAATGTAAATAAGATACGTAATGGGCAGACTTATTTGAAAGCCTCTGCTAAGGGTTGTTAATTTTCTGTTCTTCTATCACTTTATTTATTTGGTACCTTTCGGTGAATACCAAGTTACCAACCAATTATTTCCGTCTTGTAAACTATACAAGCCTAATCATCTTTCCCTGGTTTTCTGCAGAAGTACCACTTGAACCATCACTTTAGGATCCAAGATAAAGGATTTGGGATCACTTCATCACTTTGGGATAAAGTTTTTGGAACACTTCCTTCCTCAAAAGCGGATGCTAAAAGCATGTAA
Protein sequence

>MS.gene02144.t1

MVAQDFVVDLNKPLVFQVGHLGEAYEEWVHQPIVSKEGPRFFQNEVLESLTLTVWWAIPVIWLPVVCWFVYNSIQMGLSCPHIALMVVLGIFVWTLLEYTLHRFLFHIKTKTYWGNTIHYLLHGCHHKHPMDGLRLVFPPTATAILLFPFWNVVKLFSTPSTTPALFGGGLLGYVIYDCTHYYLHHGQPKTEHPRNLKKYHLNHHFRIQDKGFGITSSLWDKVFGTLPSSKADAKSM